Instruction manual Operation of the Single Measuring System

Measuring principle
BOD measurement with the OxiTop measuring system is based on pressure measurement (difference measurement). The measuring is made by pressure measurement via piezoresistive electronic pressure sensors. With the following functions, the OxiTop measuring system minimizes the measuring work and is especially suited to the courses of the respirometric BOD measurement: AUTO TEMP function: Control of the temperature adjustment and automatic start of the measurement (1 hour after starting the OxiTop measuring system at the earliest, after 3 hours at the latest). It is not necessary to temper the samples to exactly 20C before starting OxiTop. Samples between 15C and 20C can be started immediately as BOD measurement will not begin until a stable 20C is reached. This is controlled by the "AutoTemp" feature. Data logging Automatic measurement with daily storage of measured value for 5 days: facilitates measurement without supervising, e. g. over the weekend. Current value Indication of measured value (0 - 40 digits) corresponds to the digits of the previous WTW BOD instruments. Conversion of mbar to digits is stored in the instrument, i. e. evaluation of the measured values remains unchanged. Measuring range reserve from 40 to 50 digits - for overranging without reset by opening the bottle.

BOD5 determination
Normally municipal wastewater does not contain toxic or impeding substances. There are enough nutrient salts and suitable microorganisms. Under these conditions the BOD5 determination with the OxiTop measuring system is possible in the undiluted sample Required instruments and accessories OxiTop measuring system Inductive stirring system Incubator thermostatic box (temperature 20C 1K) Sample bottles brown (nominal volume 510 ml) Stirring rods Stirring rod remover suitable overflow measuring beakers Rubber quivers Sodium hydroxide tablets

BA31101e04/11.2004/As/Oxitop

Operation
Selection of sample volume Estimate the BOD5 value to be expected for the wastewater sample: Expected BOD5 value 80 % of the COD value Look for corresponding measuring range in the following chart and gather correct values for sample volume and factor. Sample volume (ml) 432 365 250 164 97 43.5 Measuring range (mg/l) 0 - 40 0 - 80 0 - 200 0 - 400 0 - 800 0 - 2000 Factor 1 2 5 10 20 50

Sample preparation
See following WTW application reports: WTW Application report 895230: "BOD measurement in household wastewater" WTW Application report 895231: "BOD measurement in organically heavily contaminated wastewater" WTW Application report 895232: "BOD measurement with presence of impeding or toxic substances"

Measurement
Information: To measure the sample volume, usually overflow measuring beakers or measuring cylinders are used. Chose the volume according to the measured value expected. Too large measuring ranges will lead to inaccurate results. To estimate the measured value you can reckon with approx. 80% of the CBS value. Sample preparation and filling of the measuring bottles compare following instruction: DIN 38409 part 52: "Measurement of the oxygen consumption" as well as WTW applicationsreports see chapter "Sample preparation and measurement". Rinse measuring bottle with sample. Empty thoroughly. Exactly measure the required oxygen-saturated (thoroughly homogenized) quantity of the sample according to information. Put the magnetic stirring rod into the bottle. Insert a rubber quiver in the neck of the bottle. Put 2 sodium hydroxide tablets into the rubber quiver with a tweezers. (Caution: The tablets must never come into the sample!) Screw OxiTop directly on sample bottle (tightly close). Start measurement: Press S and M simutaneously. (2 seconds) untill the display shows 00. Display: Stored values are deleted. Keep the measuring bottle with the OxiTop put on for 5 days at 20C (e.g. in an incubator). After the meas. temperature has been reached (after 1 hour at the earliest, after 3 hours at the latest; AutoTemp function), the OxiTop automatically starts the measurement of the oxygen consumption . During the 5 days the sample is continuously stirred. The OxiTop automatically stores one value every 24 hours for 5 days. To have the current value shown press the M key. Display current measured value: Press M until measured value is displayed (1 second).

Operation / Maintenance
Readout of the stored values after the 5 days have passed. Recall stored values: Press S until measured value is displayed (1 second). Scroll to next day by repressing the S key while the measured value is displayed (5 sec). Fast scrolling by repeatedly pressing the S key.

Convert the displayed measured value (digits) into the BOD value with the following table (Digits x Factor = BOD5 in mg/l):

Sample volume (ml) Measuring range (mg/l) 432 0 - 40 365 0 - 80 250 0 - 200 164 0 - 400 97 0 - 800 43.5 0 - 2000

Factor 1 2 5 10 20 50

Disturbances
Measured value remains below measuring range: the display shows zero or too low a value. The measuring equipment is not water-tight. Check rubber quiver, screw top and bottle. Insufficient sample pretreatment or preservation. The temperature of the sample had not sufficiently been adjusted (< 15C). Measuring range exceeded. The measuring range chosen is too small. With very high values (> 2000 mg/l) we recommend to predilute the sample. Nitrification inhibitor (allylthiourea) is missing or lacking. Errors due to procedure have not been mentioned.

System messages

Cleaning of sample bottles

Do not use disinfectants! (Disinfectants will kill the required microorganisms!) Remove gross contaminations mechanically, e.g. with a brush. Rinse the bottles with clear water or with water of the next sample.(After using detergents rinse thoroughly! Detergent residues may disturb the BOD5 determination!)

Maintenance
Cleaning of the OxiTop Single Measuring System
Do not use alcohol or acetone! Clean with a soft cloth and aqueous soap solution

Battery replacement (approx. every 3 years)

- Push in spring catch. - Open upper lid. - Remove batteries. - Put in new batteries, pay attention to polarity! (e.g. WTW-Modell Batt/Oxitop , Order No. 209 012). - Place upper lid with tap on locking pin (lower lid). Attention! Do not squeeze cable connection. - Close upper lid (make the hook click). Please dispose of the lithium batteries at a battery collection point.

Disposal note:

Information
Never use sealing grease or other lubricants for your OxiTop instruments. Some of these products contain solvents which cause severe damages on the plastic housings.The tightness of the BOD bottles is completely sufficient without grease. Rough contaminations and particles on the contact surfaces of the rubber quiver annd the OxiTop must always be wiped off. WTW grants no guarantee for damages caused by sealing grease. Labelling: To label the bottles clearly, WTW offers a marking set (6 marking rings with numbers, which can be put on the neck of the bottle): Model MARK-6 (6 pcs) Order No. 209 013 Check of the measuring system: On request WTW offer special test instruments for test control. Further literature: WTW BOD handbook DIN 38409T51 DIN 38409T52

Technical data
Measuring principle Display Measured parameter Measuring range Admissible measuring temperature Admissible sample temp. Power supply Respirometric (manometric) 2-digit 7-segment LED, 10 mm Digits 0 ... 40 digits (+10 digits for overranging) 20C 1 K 15-20C (when being filled)
Lithium batteries (260 mAh), 2 x type CR 2430 (3V) (during measurement) Protection class 3 IEC 1010 Protection system IP 54 IEC 529 EMC Emissions EN 50081-1, FCC Class A Immunity EN 50082-2, NAMUR recommend. Climate class 2, VDI/VDE 3540 Ambient Storage: -25C ... +65C temperature Operation: +20C 1K

Power consumption

Max. 25 mA

Test mark Dimensions Weight

CE H: 69 mm, 70 mm Approx. 85 g