Agilent InfinityLab LC Series

1260 Infinity II Binary Pump

User Manual

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual

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InfinityLab LC Series 1260 Infinity II Binary Pump User Manual

 In This Guide...

This manual covers the Agilent InfinityLab LC Series 1260 Infinity II Binary Pump
(G7112B).

1 Introduction
This chapter gives an introduction to the module and an instrument overview.

2 Site Requirements and Specifications

This chapter provides information about site requirements and specifications for
the binary pump.

3 Using the Pump

This chapter explains the operational parameters of the Binary Pump.

4 Optimizing Performance
This chapter gives information on how to optimize the performance of the Binary
Pump under special operational conditions.

5 Troubleshooting and Diagnostics

Overview of the troubleshooting and diagnostic features.

6 Error Information
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.

7 Test Functions and Calibration

This chapter explains all test functions that are available for the binary pump.

8 Maintenance
This chapter describes the maintenance of the module.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 3

 9 Parts and Materials for Maintenance
This chapter lists all parts and tools that are required for maintenance and simple
repairs.

10 Identifying Cables
This chapter provides information on cables used with the Agilent InfinityLab LC
Series modules.

11 Hardware Information
This chapter provides detailed technical information about your binary pump.

12 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.

13 Appendix
This chapter provides addition information on safety, legal and web.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 4

Contents

1 Introduction 10
Instrument and Operation 11
Overview of the Hydraulic Path 15
Leak and Waste Handling 20

2 Site Requirements and Specifications 25

Site Requirements 26
Physical Specifications 29
Performance Specifications 30

3 Using the Pump 32

Magnets 33
Turn on/off 34
Status Indicators 36
Best Practices 37
Leak and Waste Handling 39
Hints for Successful Use of the Binary Pump 40
Setting up the Pump with the G4208A Instant Pilot 41
Setting up the Pump with the Instrument Control Interface 42
Solvent Information 47
Algae Growth in HPLC Systems 54
Prevent Blocking of Solvent Filters 55
Normal Phase Applications 57

4 Optimizing Performance 59
When to Use a Vacuum Degasser 60
When to Use the Active Seal Wash Option 61
When to Use the Low Volume Mixer 62
When to Remove Damper and Mixer 63
How to Optimize the Compressibility Compensation Setting 65

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 5

 5 Troubleshooting and Diagnostics 68
Overview of the Module’s Indicators and Test Functions 69
User Interfaces 71
Agilent Lab Advisor Software 72

6 Error Information 73
What Are Error Messages 74
General Error Messages 75
Module Error Messages 81

7 Test Functions and Calibration 92

(System) Pressure Test 93
Valve Test 98
Binary Pump Solvent Compressibility Calibration 100
Pump Elasticity Calibration 102
Pump Leak Rate Test 104

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 6

 8 Maintenance 108
Introduction to Maintenance 109
Cautions and Warnings 110
Cleaning the Module 112
Overview of Maintenance and Simple Repairs 113
Maintenance Procedures 114
Remove and Install Doors 115
Exchange the Purge Valve Frit or the Purge Valve 117
Replace the O-Ring on the Purge Valve 120
Remove the Pump Head Assembly 122
Maintenance of a Pump Head without Seal Wash 124
Maintenance of a Pump Head with Seal Wash 127
Reinstall the Pump Head Assembly 131
Seal Wear-in Procedure 133
Exchange the Active Inlet Valve (AIV) or its Cartridge 134
Exchange the Seal Wash Cartridge 137
Replace Leak Handling System Parts 139
Exchange the Outlet Valve 141
Installation of the Solvent Selection Valve Upgrade Kit 143
Exchange the Solvent Selection Valve 145
Replacing Module Firmware 148

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 7

 9 Parts and Materials for Maintenance 149
Hydraulic Path with Solvent Selection Valve 150
Hydraulic Path without Solvent Selection Valve 152
Pump Head Assembly Without Seal Wash 154
Pump Head Assembly with Seal Wash Option 156
Outlet Valve 158
Purge Valve Assembly 159
Active Inlet Valve Assembly 160
HPLC System Tool Kit 161
Active Seal Wash Option 162
Solvent Cabinet 163
Bottle Head Assembly 164
Accessory Kit 165
Cover Parts 166

10 Identifying Cables 167

Cable Overview 168
Analog Cables 170
Remote Cables 172
CAN/LAN Cables 176
RS-232 Cable Kit 177
Agilent 1200 Module to Printer 178

11 Hardware Information 179

Firmware Description 180
Electrical Connections 183
Interfaces 185
Setting the 6-bit Configuration Switch 193
Early Maintenance Feedback 197
Instrument Layout 198

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 8

 12 LAN Configuration 199
What You Have to Do First 200
TCP/IP parameter configuration 201
Configuration Switches 202
Initialization Mode Selection 203
Dynamic Host Configuration Protocol (DHCP) 205
Manual Configuration 208

13 Appendix 212
General Safety Information 213
Waste Electrical and Electronic Equipment (WEEE) Directive 219
Radio Interference 220
Sound Emission 221
Agilent Technologies on the Internet 222

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 9

1 Introduction

Instrument and Operation 11

Introduction to the Pump 11
Principle of Operation 12
Product Description 14
Features 14
Overview of the Hydraulic Path 15
Leak and Waste Handling 20
Leak Sensor 23
Waste Concept 24

This chapter gives an introduction to the module and an instrument overview.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 10

1 Introduction
Instrument and Operation

Instrument and Operation

Introduction to the Pump

The binary pump comprises two identical pumps integrated into one housing.
Binary gradients are created by high-pressure mixing. A built-in degasser is
available for applications that require best flow stability, especially at low flow
rates, for maximum detector sensitivity. Pulse damper and mixer can be
bypassed for low flowrate applications or whenever a minimal transient volume
is desirable. Typical applications are high throughput methods with fast
gradients on high resolution 2.1 mm columns. The pump is capable of delivering
flow in the range of 0.1 – 5 mL/min against up to 600 bar. A solvent selection
valve (optional) allows to form binary mixtures (isocratic or gradient) from one of
two solvents per channel. Active seal wash (optional) is available for use with
concentrated buffer solutions.

Figure 1 Overview of the binary pump

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 11

1 Introduction
Instrument and Operation

Principle of Operation
The binary pump is based on a two-channel, dual-piston in-series design which
comprises all essential functions that a solvent delivery system has to fulfill.
Metering of solvent and delivery to the high-pressure side are performed by two
pump assemblies which can generate pressure up to 600 bar.
Each channel comprises a pump assembly including pump drive, pump head,
active inlet valve with replaceable cartridge, and outlet valve. The two channels
are fed into a low-volume mixing chamber which is connected via a restriction
capillary coil to a damping unit and a mixer. A pressure sensor monitors the
pump pressure. A purge valve with integrated PTFE frit is fitted to the pump
outlet for convenient priming of the pumping system.

to sampler

SSV
Purge valve
Seal wash pump to waste

Inlet valve Inlet valve

Damper

Outlet valve Pressure sensor Mixer Outlet valve

Pump head A Pump head B

Mixing chamber

Figure 2 The hydraulic path of the Binary Pump with damper and mixer

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 12

1 Introduction
Instrument and Operation

Damper and mixer can be bypassed for lowest delay volume of the binary pump.
This configuration is recommended for low flow rate applications with steep
gradients.
Figure 3 on page 13 illustrates the flow path in low delay volume mode. For
instructions on how to change between the two configurations, see “Convert the
Binary Pump to Low Delay Volume Mode” on page 64.

Bypassing the mixer while the damper remains in line is not a supported
NOTE configuration and may lead to undesired behavior of the binary pump.

to sampler

SSV
Purge valve
Seal wash pump to waste

Inlet valve Inlet valve

Damper

Outlet valve Pressure sensor Outlet valve

Pump head A Pump head B

Mixer

Mixing chamber

Figure 3 The hydraulic path of the Binary Pump with bypassed damper and mixer

For pump specifications, see “Performance Specifications” on page 30.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 13

1 Introduction
Instrument and Operation

Product Description
The Agilent 1260 Infinity II Binary Pump is suited for LC applications using
conventional or superficially porous particles columns, as well as STM column
technology. Gradient formation is based on a high-pressure mixing principle.
Standard or low delay volumes can be easily configured by the user. The Agilent
1260 Infinity II Binary Pump offers reproducible gradients and high-performance,
providing high-throughput and fast separations. The pump is suitable for routine
applications, with UV or MS detection, where high speed and resolution with
uncompromised data quality are required.

Status indicator

Active seal wash

Purge valve

Degasser

Pump head A

Pump head B
Leak drain
Power switch

Figure 4 Overview of the pump

Features
• Configurable delay volume - down to 120 µL together with a flow range up to
5 mL/min provides universal applicability.
• Change from standard to low delay volume configuration is enabled.
• High gradient performance - even at low % B and narrow-bore flow rates.
• Integrated 2-channel-degasser.
• Fast and precise gradients using LC/MS, as well as UV-only systems.
• Fully exploits the speed and separation potential of Poroshell.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 14

1 Introduction
Overview of the Hydraulic Path

Overview of the Hydraulic Path

The solvent from the bottle in the solvent cabinet enters the pump through an
active inlet valve. Each side of the binary pump comprises two substantially
identical pump units. Both pump units comprise a ball-screw drive and a pump
head with two sapphire pistons for reciprocating movement.

From solvent bottle

Inlet valve /degasser/SSV

Seal

Outlet valve

Piston

To mixing chamber

Figure 5 Pump head

A servo-controlled variable reluctance motor drives the two ball-screw drives in

opposite directions. The gears for the ball-screw drives have different
circumferences (ratio 2:1) allowing the first piston to move at double the speed of
the second piston. The solvent enters the pump heads close to the bottom limit
and leaves it at its top. The outer diameter of the piston is smaller than the inner
diameter of the pump-head chamber allowing the solvent to fill the gap in
between. The first piston has a stroke volume in the range of 20 µL to 100 µL
depending on the flow rate. The microprocessor controls all flow rates in a range

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 15

1 Introduction
Overview of the Hydraulic Path

of 1 µL/min to 5 mL/min. The inlet of the first pumping unit is connected to the
active inlet valve which is processor-controlled opened or closed allowing solvent
to be drawn into the first pump unit.
The outlet of the first pump chamber is connected by a 500 µL absorber capillary
to the second pump chamber. The outlets of the second chambers of both pump
channels joined via a small mixing chamber. A coiled restriction capillary
connects the mixing chamber via a pressure pulse damper, a mixer and a
pressure sensor to the purge valve assembly. The outlet of the purge valve
assembly is then connected to the attached chromatographic system.
When turned on, the pump runs through an initialization procedure to determine
the upper dead center of the first piston of both pump channels. The first piston
moves slowly upwards to the mechanical stop of the pump head and from there
it moves back a predetermined path length. The controller stores this piston
position in memory. After this initialization the pump starts operation with the set
parameters for the two pump channels.
The active inlet valve is opened and the down moving piston draws solvent into
the first pump head. At the same time the second piston is moving upwards
delivering into the system. After a controller defined stroke length (depending on
the flow rate) the drive motors are stopped and the active inlet valve is closed.
The motor direction is reversed and moves the first piston up until it reaches the
stored upper limit and at the same time moving the second piston downwards.
Then the sequence starts again moving the pistons up and down between the
two limits. During the delivery stroke of the first piston the solvent in the pump
head is pressed through the outlet valve into the second pumping unit. The
second piston draws in half of the volume displaced by the first piston and the
remaining half volume is directly delivered into the system. During the drawing
stroke of the first piston, the second piston delivers the drawn volume into the
system.
For pump specifications, see “Performance Specifications” on page 30.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 16

1 Introduction
Overview of the Hydraulic Path

What is Pump Elasticity Compensation?

The flow path of the pump consists of pump chambers, sapphire pistons,
polymer seals, stainless steel tubing of different dimension, pressure sensor, and
so forth. All of these parts deform when pressurized. The sum of this
deformation is called pump elasticity.
Let us look at a practical example: Piston 1 draws solvent at ambient pressure.
The movement direction is reversed and the piston 1 now compresses the
solvent until the operating pressure of the HPLC system is reached. The outlet
valve opens, and solvent is pumped by piston 1 into pump chamber 2. Due to two
factors, the solvent volume that is delivered into the system at high pressure is
smaller than it is supposed to be:
1 The solvent is compressible
2 The pump has a certain elasticity which causes its internal volume to increase
with pressure.
In order to compensate for these two influences, their contributions must be
known. An elasticity calibration allows separating pump properties from solvent
properties and therefore allows transferring solvent properties, which have been
obtained from one pump to another pump with different elasticity.
Elasticity calibration is done with a solvent, which properties (compressibility,
thermal expansion) are well-known and documented: pure water. When pumping
water and using its property data for controlling the pump, any deviations from
the theoretical pressure profile during solvent recompression are caused by the
elasticity of the pump.
The Pump Elasticity Calibration calculates correction factors to compensate for
the individual elasticity of the pump that is being calibrated. The elasticity is
different for every pump and may change with the replacement of parts in the
flow path, e.g. pump seals.
All binary pumps are elasticity calibrated at the factory and require recalibration
only after preventive maintenance or major repairs to the flow path. Replacement
of capillaries or PTFE frits are not considered as a major repair.

Incorrect pump elasticity calibration.

C AU T I O N
Solvent compressibility calibrations acquired with a miscalibrated pump will
work, but they are not transferable to other pumps. A correct pump elasticity
calibration is an essential prerequisite for successful solvent compressibility
calibrations.
 Calibrate the pump elasticity correctly.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 17

1 Introduction
Overview of the Hydraulic Path

What is Solvent Compressibility Compensation?

Although the compressibility of liquids is orders of magnitude lower than the
compressibility of gases, without correction a noticeable volume error would be
seen if typical chromatographic solvents are compressed to operating pressures
as high as 600 bar. In addition, the compressibility depends on pressure,
temperature and the amount of dissolved gas. In order to minimize the influence
of the latter, the use of a vacuum degasser is mandatory for a high flow and
composition precision. Unfortunately, the influence of the temperature on
compressibility is non-linear and cannot be calculated.
The Agilent 1260 Infinity II Binary Pump features a multi point compressibility
calibration. The compressibility of a solvent is determined at different pressures
from 0 – 600 bar and stored in an XML file. This file can be distributed to other
pumps because the solvent compressibility is independent from the pump.
The binary pump and ChemStation come with predetermined solvent
compressibility data for the most common HPLC solvents like water, acetonitrile,
methanol, etc. Users can calibrate their own solvent mixtures with the help of an
easy to use calibration procedure in the Agilent Lab Advisor software.
Let us use the practical example from the last section once again to understand
how compressibility compensation works:
Piston 1 draws solvent at ambient pressure. The movement direction is reversed
and piston 1 now compresses the solvent until the operating pressure of the
HPLC system is reached. The outlet valve opens, and solvent is pumped by
piston 1 into pump chamber 2.
Without any compensation, the delivered volume at operating pressure would be
too low. In addition, it would take a noticeable amount of time to recompress the
solvent to operating pressure. During this time frame, no solvent would be
delivered into the system and as a result a high pressure fluctuation (known as
pressure ripple) would be observed.
When both solvent compressibility at the current operating pressure and pump
elasticity are known, the pump can automatically correct for the missing volume
by drawing the appropriate larger solvent volume at ambient pressure and speed
up the piston during the recompression phase in the first pump chamber. As a
result, the pump delivers the accurate volume with any (calibrated) solvent at any
pressure at a greatly reduced pressure ripple. For applications that require lowest
transition volume of the pump, damper and mixer can be bypassed.
For compatibility with older methods from G1312A Binary Pumps, the previous
one-point compressibility compensation is available, too. However, since the
compressibility is a non-linear function, one single compressibility value per
solvent will only give good results at one particular pressure.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 18

1 Introduction
Overview of the Hydraulic Path

How Does Variable Stroke Volume Work?

The smaller the solvent volume in the pump chamber is, the faster it can be
recompressed to operating pressure. The binary pump allows to manually or
automatically adjust the pump stroke volume of the first piston in the range of
20 – 100 µL. Due to the compression of the solvent volume in the first pump
chamber, each piston stroke of the pump will generate a small pressure
pulsation, influencing the flow ripple of the pump. The amplitude of the pressure
pulsation mainly depends on the stroke volume and the compressibility
compensation for the solvent in use. Small stroke volumes generate less
pressure pulsation than larger stroke volumes at the same flow rate. In addition,
the frequency of the pressure pulsation will be higher. This will decrease the
influence of flow pulsations on retention times.
In gradient mode, a smaller stroke volume results in less flow ripple and reduces
the composition ripple.
The binary pump uses a processor-controlled ball screw system for driving its
pistons. The normal stroke volume is optimized for the selected flow rate. Small
flow rates use a small stroke volume while higher flow rates use a higher stroke
volume.
The stroke volume for the pump is by default set to AUTO mode. This means that
the stroke is optimized for the flow rate in use. A change to larger stroke volumes
is possible but not recommended.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 19

1 Introduction
Leak and Waste Handling

Leak and Waste Handling

The Agilent InfinityLab LC Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
The solvent cabinet is designed to store a maximum volume of 8 L solvent. The
maximum volume for an individual bottle stored in the solvent cabinet should not
exceed 2 L. For details, see the usage guideline for the Agilent Infinity II Solvent
Cabinets (a printed copy of the guideline has been shipped with the solvent
cabinet, electronic copies are available on the Internet).
All leak plane outlets are situated in a consistent position so that all Infinity and
Infinity II modules can be stacked on top of each other. Waste tubes are guided
through a channel on the right hand side of the instrument, keeping the front
access clear from tubes.
The leak plane provides leak management by catching all internal liquid leaks,
guiding them to the leak sensor for leak detection, and passing them on to the
next module below, if the leak sensor fails. The leak sensor in the leak plane
stops the running system as soon as the leak detection level is reached.
Solvent and condensate is guided through the waste channel into the waste
container:
• from the detector's flow cell outlet
• from the Multisampler needle wash port
• from the Sample Cooler or Sample Thermostat (condensate)
• from the pump's Seal Wash Sensor (if applicable)
• from the pump's Purge Valve or Multipurpose Valve

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 20

1 Introduction
Leak and Waste Handling

Figure 6 Infinity II Leak Waste Concept (Flex Bench installation)

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 21

1 Introduction
Leak and Waste Handling

Figure 7 Infinity II Single Stack Leak Waste Concept (bench installation)

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 22

1 Introduction
Leak and Waste Handling

Figure 8 Infinity II Two Stack Leak Waste Concept (bench installation)

The waste tube connected to the leak plane outlet on each of the bottom
instruments guides the solvent to a suitable waste container.

Leak Sensor
Solvent incompatibility
C AU T I O N
The solvent DMF (dimethylformamide) leads to corrosion of the leak sensor.
The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible
with DMF.
 Do not use DMF as mobile phase.
 Check the leak sensor regularly for corrosion.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 23

1 Introduction
Leak and Waste Handling

Waste Concept
1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with
4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use
your own waste solution, make sure that the tubes don't immerse in the liquid.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 24

2 Site Requirements and Specifications

Site Requirements 26
Physical Specifications 29
Performance Specifications 30

This chapter provides information about site requirements and specifications for
the binary pump.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 25

2 Site Requirements and Specifications
Site Requirements

Site Requirements

A suitable environment is important to ensure optimal performance of the

instrument.

Power Considerations
The module power supply has wide ranging capability. It accepts any line voltage
in the range described in Table 1 on page 29. Consequently there is no voltage
selector in the rear of the module. There are also no externally accessible fuses,
because automatic electronic fuses are implemented in the power supply.

Incorrect line voltage at the module

WAR N IN G
Shock hazard or damage of your instrument can result if the devices are
connected to line voltage higher than specified.
 Connect your module to the specified line voltage.

Module is partially energized when switched off, as long as the power cord
WAR N IN G
is plugged in.
Repair work at the module can lead to personal injuries, e.g. shock hazard,
when the cover is opened and the module is connected to power.
 Make sure that it is always possible to access the power plug.
 Remove the power cable from the instrument before opening the cover.
 Do not connect the power cable to the Instrument while the covers are
removed.

Inaccessible power plug.

WAR N IN G
In case of emergency it must be possible to disconnect the instrument
from the power line at any time.
 Make sure the power connector of the instrument can be easily reached
and unplugged.
 Provide sufficient space behind the power socket of the instrument to
unplug the cable.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 26

2 Site Requirements and Specifications
Site Requirements

Power Cords
Country-specific power cords are available for the module. The female end of all
power cords is identical. It plugs into the power-input socket at the rear. The male
end of each power cord is different and designed to match the wall socket of a
particular country or region.
Agilent makes sure that your instrument is shipped with the power cord that is
suitable for your particular country or region.

Unintended use of power cords

WAR N IN G
Using power cords for unintended purposes can lead to personal injury or
damage of electronic equipment.
 Never use a power cord other than the one that Agilent shipped with this
instrument.
 Never use the power cords that Agilent Technologies supplies with this
instrument for any other equipment.
 Never use cables other than the ones supplied by Agilent Technologies
to ensure proper functionality and compliance with safety or EMC
regulations.

Absence of ground connection

WAR N IN G
The absence of ground connection can lead to electric shock or short
circuit.
 Never operate your instrumentation from a power outlet that has no
ground connection.

Electrical shock hazard

WAR N IN G
Solvents may damage electrical cables.
 Prevent electrical cables from getting in contact with solvents.
 Exchange electrical cables after contact with solvents.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 27

2 Site Requirements and Specifications
Site Requirements

Bench Space
The module dimensions and weight (see Table 1 on page 29) allow you to place
the module on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches)
in the rear for air circulation and electric connections.
If the bench shall carry a complete HPLC system, make sure that the bench is
designed to bear the weight of all modules.
The module should be operated in a horizontal position.

Agilent recommends that you install the HPLC instrument in the InfinityLab Flex
NOTE Bench rack. This option helps to save bench space as all modules can be placed
into one single stack. It also allows to easily relocate the instrument to another
lab.

Condensation
Condensation within the module
C AU T I O N
Condensation can damage the system electronics.
 Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
 If your module was shipped in cold weather, leave it in its box and allow it to
warm slowly to room temperature to avoid condensation.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 28

2 Site Requirements and Specifications
Physical Specifications

Physical Specifications

Table 1 Physical Specifications G7112B

Type Specification Comments

Weight 17.6 kg (38.8 lbs)

Dimensions 180 x 396 x 436 mm

(height × width × depth) (7.1 x 15.6 x 17.2 inches)

Line voltage 100 – 240 V~, ± 10 % Wide-ranging capability

Line frequency 50 or 60 Hz, ± 5 %

Power consumption 90 VA, 74 W

Ambient operating 4–55 °C (39–131 °F)

temperature

Ambient non-operating -40 – 70 °C (-40 – 158 °F)

temperature

Humidity < 95 % r.h. at 40 °C (104 °F) Non-condensing

Operating altitude Up to 3000 m (9842 ft)

Safety standards: Overvoltage category II, Pollution degree 2 For indoor use only
IEC, EN, CSA, UL

ISM Classification ISM Group 1 Class B According to CISPR 11

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 29

2 Site Requirements and Specifications
Performance Specifications

Performance Specifications

Table 2 Performance Specifications G7112B

Type Specification Comments

Hydraulic system Two dual piston in series pumps with

servo-controlled variable stroke drive,
power transmission by gears and ball
screws, floating pistons

Flow range settable: 0.001 – 5 mL/min Set points in 0.001 mL/min

recommended: 0.05 – 5.0 mL/min increments

Flow precision ≤0.07 % RSD or < 0.02 min SD, whichever is Based on retention time at
greater constant temperature

Flow accuracy ± 1 % or 10 µL/min, whichever is greater Pumping degassed H2O at

10 MPa (100 bar, 1450 psi)

Pressure operating Up to 60 MPa (600 bar, 8702 psi) up to

range 5 mL/min

Pressure pulsation < 2 % amplitude (typically < 1.3 %), or

< 0.3 MPa (3 bar, 44 psi), whichever is
greater
Low delay volume configuration:
< 5 % amplitude (typically < 2 %)

Compressibility Pre-defined, based on mobile phase

compensation compressibility

Recommended pH 1.0 – 12.5 Solvents with pH < 2.3 should

range not contain acids that attack
stainless steel

Gradient formation High-pressure binary mixing

Delay volume Standard delay volume Measured with water at

configuration:600 – 900 µL, (includes 1 mL/min (water/water with
400 µL mixer), dependent on back pressure tracer)

Low delay volume configuration:120 µL

Composition range Settable: 0 – 100 %

Recommended: 1 – 99 % or 5 µL/min per
channel, whichever is greater

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2 Site Requirements and Specifications
Performance Specifications

Table 2 Performance Specifications G7112B

Type Specification Comments

Composition precision < 0.15 % RSD or < 0.04 min SD, whichever At 0.2 and 1 mL/min; based on
is greater retention time at constant
temperature

Composition accuracy ± 0.35 % absolute At 2 mL/min, at 10 MPa

(100 bar, 1450 psi)
(water/water with tracer)

Integrated degassing Number of channels: 2

unit Internal volume per channel: 0.45 mL

Instrument control LC & CE Drivers A.02.14 or above For details about supported
Instrument Control Framework (ICF) software versions refer to the
A.02.04 or above compatibility matrix of your
InfinityLab LC Companion (G7108AA) with version of the LC and CE
firmware D.07.25 or above Drivers
Instant Pilot (G4208A) with firmware
B.02.20 or above
Lab Advisor software B.02.08 or above

Communication Controller Area Network (CAN)

Local Area Network (LAN)
Extended Remote Interface (ERI)
Universal Serial Bus (USB)

Safety and Extensive diagnostics, error detection and

maintenance display through Agilent Lab Advisor, leak
detection, safe leak handling, leak output
signal for shutdown of the pumping
system. Low voltage in major maintenance
areas.

GLP features Early maintenance feedback (EMF) for

continuous tracking of instrument usage in
terms of seal wear and volume of pumped
mobile phase with pre-defined and user
settable limits and feedback messages.
Electronic records of maintenance and
errors.

Housing All materials are recyclable

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3 Using the Pump

Magnets 33
Turn on/off 34
Status Indicators 36
Best Practices 37
Daily / Weekly tasks 37
Power up / Shut-down the pump 37
Prepare the pump 38
How to deal with solvents 38
Leak and Waste Handling 39
Hints for Successful Use of the Binary Pump 40
Setting up the Pump with the G4208A Instant Pilot 41
Setting up the Pump with the Instrument Control Interface 42
Overview 42
Setup of Basic Pump Parameters 43
Pump Control 44
Auxiliary Pump Parameters 44
Data Curves 45
Bottle Filling 46
Solvent Information 47
Algae Growth in HPLC Systems 54
How to Prevent and/or Reduce the Algae Problem 54
Prevent Blocking of Solvent Filters 55
Checking the Solvent Filters 56
Cleaning the Solvent Filters 56
Normal Phase Applications 57

This chapter explains the operational parameters of the Binary Pump.

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3 Using the Pump
Magnets

Magnets

1 Magnets in doors of pumps, autosamplers, detectors, and fraction collectors.

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3 Using the Pump
Turn on/off

Turn on/off

This procedure exemplarily shows an arbitrary LC stack configuration.

1 2

Power switch: On

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3 Using the Pump
Turn on/off

3 Turn instrument On/Off with the control software. 4

Power switch: Off

5

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3 Using the Pump
Status Indicators

Status Indicators

This procedure exemplarily shows an arbitrary LC stack configuration.

1 The module status indicator indicates one of six possible module conditions:

Status indicators
1. Idle
2. Run mode
3. Not-ready. Waiting for a specific pre-run condition to be reached or
completed.
4. Error mode - interrupts the analysis and requires attention (for example, a
leak or defective internal components).
5. Resident mode (blinking) - for example, during update of main firmware.
6. Bootloader mode (fast blinking). Try to re-boot the module or try a
cold-start. Then try a firmware update.

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3 Using the Pump
Best Practices

Best Practices

Daily / Weekly tasks

Daily tasks
• Replace mobile phase based on water/buffer.
• Replace organic mobile phase latest every second day.
• Check seal wash solvent.

Weekly Tasks
• Change seal wash solvent (10 % isopropanol in water) and bottle.
• If applications with salts were used, flush all channels with water and remove
possible salt deposits manually.
• Inspect solvent filters for dirt or blockages. Exchange if no flow is coming out
of the solvent line when removed from the degasser inlet.

Power up / Shut-down the pump

Power up the pump
• Use new or different mobile phase (as required).
• Purge pump heads with 2.5 – 3 mL/min for 5 min.
• Stabilize the system by running for 10 – 20 min.

Long-term shut-down of the system

• Flush system with water to remove buffer.
• Remove all samples from the sampler and store according to good laboratory
practice.
• Use recommended solvents to store the system.
• Power off the system.

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3 Using the Pump
Best Practices

Prepare the pump

Purge
Use the Purge function to:
• fill the pump,
• exchange a solvent,
• remove air bubbles in tubes and pump heads.

Seal wash
Seal Wash guarantees a maximum seal life time. Use Seal Wash:
• When using buffers with elevated salt concentrations
• When using volatile solvents with non-volatile additives

Contaminated seal wash solvent

C AU T I O N
 Do not recycle seal wash solvent to avoid contamination.
 Weekly exchange seal wash solvent.

How to deal with solvents

• Use clean bottles only.
• Exchange water-based solvents daily.
• Select solvent volume to be used up within 1 – 2 days.
• Use only HPLC-grade solvents and water filtered through 0.2 µm filters.
• Label bottles correctly with bottle content, and filling date / expiry date.
• Use solvent inlet filters.
• Reduce risk of algae growth: use brown bottles for aqueous solvents, avoid
direct sunlight.

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Leak and Waste Handling

Leak and Waste Handling

Toxic, flammable and hazardous solvents, samples and reagents
WAR N IN G
The handling of solvents, samples and reagents can hold health and safety
risks.
 When working with these substances observe appropriate safety
procedures (for example by wearing goggles, safety gloves and
protective clothing) as described in the material handling and safety
data sheet supplied by the vendor, and follow good laboratory practice.
 Do not use solvents with an auto-ignition temperature below 200 °C
(392 °F). Do not use solvents with a boiling point below 56 °C (133 °F).
 Avoid high vapor concentrations. Keep the solvent temperature at least
40 °C (72 °F) below the boiling point of the solvent used. This includes
the solvent temperature in the sample compartment. For the solvents
methanol and ethanol keep the solvent temperature at least 25 °C
(45 °F) below the boiling point.
 Do not operate the instrument in an explosive atmosphere.
 Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for
example, carbon disulfide).
 Reduce the volume of substances to the minimum required for the
analysis.
 Never exceed the maximum permissible volume of solvents (8 L) in the
solvent cabinet. Do not use bottles that exceed the maximum
permissible volume as specified in the usage guideline for solvent
cabinet.
 Ground the waste container.
 Regularly check the filling level of the waste container. The residual free
volume in the waste container must be large enough to collect the waste
liquid.
 To achieve maximal safety, regularly check the tubing for correct
installation.

For details, see the usage guideline for the solvent cabinet. A printed copy of the
NOTE guideline has been shipped with the solvent cabinet, electronic copies are
available in the Agilent Information Center or via the Internet.

For details on correct installation, see separate installation documentation.

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3 Using the Pump
Hints for Successful Use of the Binary Pump

Hints for Successful Use of the Binary Pump

• Place solvent cabinet with the solvent bottles always on top (or at a higher
level) of the pump.
• Flush the degasser with at least 5 mL per channel before operating the pump,
especially when the pumping system had been turned off for a certain length
of time (for example, overnight) and volatile solvent mixtures are used in the
channels.
• Prevent blocking of solvent inlet filters (never use the pump without solvent
inlet filters). Growth of algae should be avoided (see “Prevent Blocking of
Solvent Filters” on page 55).
• Check purge valve frit and column frit in regular time intervals. A blocked
purge valve frit can be identified by black, yellow or greenish layers on its
surface or by a pressure greater than 10 bar in low delay volume configuration
and 20 bar in standard configuration when pumping distilled water at a rate of
5 mL/min with an open purge valve.
• Whenever possible use a minimum flow rate of 5 µL/min per solvent channel
to avoid crossflow of solvent into the unused pump channel.
• Whenever exchanging the pump seals, the purge valve frit should be
exchanged, too.
• When using buffer solutions, flush the system with water before switching it
off. The seal wash option should be used when installed, especially when
buffer solutions with concentrations of 0.1 M or higher are being pumped for
long periods of time.
• Check the pump pistons for scratches, grooves and dents when changing the
piston seals. Damaged pistons cause micro leaks and will decrease the
lifetime of the seals.
• After changing the piston seals, apply the seal wear-in procedure (see “Seal
Wear-in Procedure” on page 133).
• Place the aqueous solvent on channel A and the organic solvent on channel B.
The default compressibility settings are set accordingly.

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3 Using the Pump
Setting up the Pump with the G4208A Instant Pilot

Setting up the Pump with the G4208A Instant

Pilot

Generic operation of the G4208A Instant Pilot is covered in the Agilent Instant
Pilot G4208A User's Guide (G4208-90006). Details about setting up module
specific parameters can be found in the Instant Pilot online help.
The pump parameters are described in detail in “Overview” on page 42.

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3 Using the Pump
Setting up the Pump with the Instrument Control Interface

Setting up the Pump with the Instrument Control

Interface

Overview
Parameters described in following sections are offered by the instrument control
interface and can usually be accessed through Agilent instrument control
software. For details, please refer to manuals and online help of respective user
interfaces.

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3 Using the Pump
Setting up the Pump with the Instrument Control Interface

Setup of Basic Pump Parameters

The most important parameters of the pump are listed in Table 3 on page 43.
Table 3 Basic pump parameters

Parameter Limits Description

• Flow 0.001 – 5 mL/min Total flow rate of the pump. See “When to Remove Damper and Mixer” on page 63
for pump hardware modifications to achieve lowest delay volume.

• Stop Time 0.01 min - no limit The stop time of the pump usually controls the run time of the whole LC system.
Use no limit to stop the run manually (useful for method development).

• Post Time off - 99999 min Time between the end of a run and the start of the next. Used for column
equilibration after a gradient.

• Pressure Max: 0 – 600 bar Max must be bigger than Min! Set max pressure to the maximum operating
Limits Min: 0 – 600 bar pressure of your column. A min pressure setting of e.g. 10 bar will turn off your
pump automatically when running out of solvent. A smarter way, however, is to use
the bottle fillings function (see “Bottle Filling” on page 46).

• Solvent A 0 – 100 % Although channel A can be set to 0 %, it cannot be turned off. This channel should
be used for the aqueous phase (water).

• Solvent B off - 100 % The percentage of channel B is automatically complemented by channel A to give
100 %.

• Solvent H2O, ACN, MeOH, IPA Select the solvent you are using in the respective solvent channel from the
type drop-down list. In case your solvent is not listed, perform a solvent compressibility
calibration (see “Running the Solvent Compressibility Calibration” on page 101).
For details on solvent compressibility see “Binary Pump Solvent Compressibility
Calibration” on page 100.

• Solvent Free text field for a description of the solvent. This description will show up in
Comment method printouts, etc.

• Timetable max. number of lines Use the timetable to build solvent gradients, flow gradients, or combinations of
depends on free space both. Gradients are always linear. Use multiple timetable entries to mimic
in pump memory exponential or parabolic gradients.

• Display There are three ways to display the timetable:

• in tabular form
• as flow/pressure graph
• as solvent percentage plot
Values can only be changed in tabular view.

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Setting up the Pump with the Instrument Control Interface

Pump Control
The pump can be switched between following states: On, Off or to Standby. In
Standby, the pump motor is still controlled. When the pump is switched on from
standby, it does not re-initialize.

Upon initialization, the pump ignores the Maximum Flow Gradient value.
C AU T I O N
This can result in a rapid and uncontrolled pressure increase.
 To prevent harm to the column, open the purge valve until the initialization
is finished.

The optional seal wash pump can be controlled by either switching it off, using it
for a single time or specifying frequency and duration of periodic wash intervals.

Auxiliary Pump Parameters

The auxiliary pump parameters are pre-set to fit most applications. Adjustments
should only be made when required. Table 4 on page 45 shows the available
auxiliary parameters with their default values.

Upon initialization, the pump ignores the Maximum Flow Gradient value.
C AU T I O N
This can result in a rapid and uncontrolled pressure increase.
 To prevent harm to the column, open the purge valve until the initialization
is finished.

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Setting up the Pump with the Instrument Control Interface

Table 4 Auxiliary pump parameters

Parameter Limits Description

• Maximum Flow 0.1 – 100 mL/min² With this parameter flow rate changes can be ramped up and down slowly
Gradient default: 100 mL/min² to avoid pressure shocks to the column. The default value is 100 mL/min²
which in fact turns the function off.

• Minimum Stroke 20 – 100 µL The volume one pump piston delivers per stroke. In general, a smaller stroke
default: Auto volume results in lower pump ripple. The Auto setting adjusts the strokes
dynamically to the lowest possible value.
The strokes can be set individually for pump heads A and B.

• Compressibility 0 - 150·10-6 /bar or For best performance, check option Use enhanced compressibility
enhanced calibration. With this option, the pump will use solvent data libraries provided
compressibility by Agilent or data generated by using solvent compressibility calibrations
calibration (see “Running the Solvent Compressibility Calibration” on page 101).
default: use enhanced For details on solvent compressibility see “Binary Pump Solvent
comp. calibration Compressibility Calibration” on page 100.
For backward compatibility to 400 bar pumps, the solvent compressibility
can still be set manually for each channel when the box is unticked.

Data Curves
The binary pump provides the possibility to store the following operational data in
the data file of the Agilent data system:
• Solvent percentage for each channel,
• pump flow,
• pressure

The pressure data curve is generated from the pressure sensor readings, while
NOTE %A, %B and flow are calculated from the method settings of the pump.

For details, please refer to the online help or manual of your instrument control
software.

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Setting up the Pump with the Instrument Control Interface

Bottle Filling
The pump offers a powerful feature to monitor the liquid level in the solvent
bottles. With total bottle volume and initial filling volume set correctly, the pump
subtracts the consumed volume continuously from the initial value and stops the
pump and method/sequence execution before the system runs dry or an analysis
is corrupted.

The bottle filling feature fails if multiple solvent inlets are put into one solvent
C AU T I O N bottle!
 In that case implement a minimum pressure limit to avoid that the pump
runs dry when solvents are empty.

Table 5 on page 46 lists the available bottle filling parameters.

Table 5 Bottle Filling Parameters

Parameter Limits Description

• Total Volume 0 – 1000 L This is the capacity (maximum possible volume) in liter of the solvent bottle. In
default: 0 L combination with the actual volume, this parameter is used for calculating and
displaying the relative liquid level.

• Actual 0 – 1000 L After filling the solvent bottles, enter the actual volumes into these boxes. The
Volume default: 0 L Actual Volume must not be larger than the Total Volume of the bottle.

• Prevent default: unchecked If this option is checked, the pump won’t start a new run if the solvent level in
analysis....... one or more bottles is below the minimum volume. Enter a minimum volume in
liter, which considers the position of the solvent inlet and size/shape of the
solvent bottle such that no air is drawn if the actual volume gets close to this
limit.

• Turn pump default: unchecked If this option is checked, the pump will turn off before air is aspirated. However,
off... the residual solvent volume has been calculated for 1 L solvent bottles and may
be too small for large bottles or other vessels.

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3 Using the Pump
Solvent Information

Solvent Information

Observe the following recommendations on the use of solvents.

• Follow the recommendations for avoiding the growth of algae, see “Algae
Growth in HPLC Systems” on page 54
• Small particles can permanently block capillaries and valves. Therefore,
always filter solvents through 0.22 µm filters.
• Avoid or minimize the use of solvents that may corrode parts in the flow path.
Consider specifications for the pH range given for different materials such as
flow cells, valve materials etc. and recommendations in subsequent sections.

Materials in Flow Path

Following materials are used in the flow path of this module:
Table 6 Materials in flow path

Part Materials

Degasser chamber TFE/PDD Copolymer, PFA (internal tubings), PEEK (inlets), FEP
(tubings), ETFE (fittings)

SSV PEEK, FFKM

Active inlet valve SST, sapphire, ruby, ceramic, PTFE

Outlet valve SST, gold, ruby, ZrO2-based ceramic, tantalum

Adapter SST, gold

Pump head (body) SST

Pistons Sapphire

Piston seals/wash seals PTFE, SST (reversed phase) or UHMW-PE, SST (normal phase)

Pressure sensor SST

Purge valve SST, gold, PTFE, ceramic

Damping unit SST, gold

Capillaries/fittings SST

Tubings PTFE

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Solvent Information

General Information about Solvent/Material

Compatibility
Materials in the flow path are carefully selected based on Agilent’s experiences in
developing highest-quality instruments for HPLC analysis over several decades.
These materials exhibit excellent robustness under typical HPLC conditions. For
any special condition, please consult the material information section or contact
Agilent.

Disclaimer
Subsequent data was collected from external resources and is meant as a
reference. Agilent cannot guarantee the correctness and completeness of such
information. Data is based on compatibility libraries, which are not specific for
estimating the long-term life time under specific but highly variable conditions of
UHPLC systems, solvents, solvent mixtures and samples. Information can also
not be generalized due to catalytic effects of impurities like metal ions,
complexing agents, oxygen etc. Apart from pure chemical corrosion, other
effects like electro corrosion, electrostatic charging (especially for
non-conductive organic solvents), swelling of polymer parts etc. need to be
considered. Most data available refers to room temperature (typically 20 – 25 °C,
68 – 77 °F). If corrosion is possible, it usually accelerates at higher temperatures.
If in doubt, please consult technical literature on chemical compatibility of
materials.

MP35N
MP35N is a nonmagnetic, nickel-cobalt-chromium-molybdenum alloy
demonstrating excellent corrosion resistance (for example, against nitric and
sulfuric acids, sodium hydroxide, and seawater) over a wide range of
concentrations and temperatures. In addition, this alloy shows exceptional
resistance to high-temperature oxidation. Due to excellent chemical resistance
and toughness, the alloy is used in diverse applications: dental products, medical
devices, nonmagnetic electrical components, chemical and food processing
equipment, marine equipment. Treatment of MP35N alloy samples with 10 %
NaCl in HCl (pH 2.0) does not reveal any detectable corrosion. MP35N also
demonstrates excellent corrosion resistance in a humid environment. Although
the influence of a broad variety of solvents and conditions has been tested, users
should keep in mind that multiple factors can affect corrosion rates, such as
temperature, concentration, pH, impurities, stress, surface finish, and dissimilar
metal contacts.

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Solvent Information

Polyphenylene Sulfide (PPS)

Polyphenylene sulfide has outstanding stability even at elevated temperatures. It
is resistant to dilute solutions of most inorganic acids, but it can be attacked by
some organic compounds and oxidizing reagents. Nonoxidizing inorganic acids,
such as sulfuric acid and phosphoric acid, have little effect on polyphenylene
sulfide, but at high concentrations and temperatures, they can still cause material
damage. Nonoxidizing organic chemicals generally have little effect on
polyphenylene sulfide stability, but amines, aromatic compounds, and
halogenated compounds may cause some swelling and softening over extended
periods of time at elevated temperatures. Strong oxidizing acids, such as nitric
acid (> 0.1 %), hydrogen halides (> 0.1 %), peroxy acids (> 1 %), or chlorosulfuric
acid degrade polyphenylene sulfide. It is not recommended to use polyphenylene
sulfide with oxidizing material, such as sodium hypochlorite and hydrogen
peroxide. However, under mild environmental conditions, at low concentrations
and for short exposure times, polyphenylene sulfide can withstand these
chemicals, for example, as ingredients of common disinfectant solutions.

PEEK
PEEK (Polyether-Ether Ketones) combines excellent properties regarding
biocompatibility, chemical resistance, mechanical and thermal stability. PEEK is
therefore the material of choice for UHPLC and biochemical instrumentation.
It is stable in the specified pH range (for the Bio-Inert LC system: pH 1 – 13, see
bio-inert module manuals for details), and inert to many common solvents.
There is still a number of known incompatibilities with chemicals such as
chloroform, methylene chloride, THF, DMSO, strong acids (nitric acid > 10 %,
sulfuric acid > 10 %, sulfonic acids, trichloroacetic acid), halogens or aqueous
halogen solutions, phenol and derivatives (cresols, salicylic acid, and so on).
When used above room temperature, PEEK is sensitive to bases and various
organic solvents, which can cause it to swell. Under such conditions, normal
PEEK capillaries are sensitive to high pressure. Therefore, Agilent uses stainless
steel cladded PEEK capillaries in bio-inert systems. The use of stainless steel
cladded PEEK capillaries keeps the flow path free of steel and ensures pressure
stability up to 600 bar. If in doubt, consult the available literature about the
chemical compatibility of PEEK.

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Solvent Information

Polyimide
Agilent uses semi-crystalline polyimide for rotor seals in valves and needle seats
in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as
Vespel, which is also used by Agilent.
Polyimide is stable in a pH range between 1 and 10 and in most organic solvents.
It is incompatible with concentrated mineral acids (e.g. sulphuric acid), glacial
acetic acid, DMSO and THF. It is also degraded by nucleophilic substances like
ammonia (e.g. ammonium salts in basic conditions) or acetates.

Polyethylene (PE)
Agilent uses UHMW (ultra-high molecular weight)-PE/PTFE blends for yellow
piston and wash seals, which are used in 1290 Infinity pumps, 1290 Infinity II
pumps, the G7104C and for normal phase applications in 1260 Infinity pumps.
Polyethylene has a good stability for most common inorganic solvents including
acids and bases in a pH range of 1 to 12.5. It is compatible with many organic
solvents used in chromatographic systems like methanol, acetonitrile and
isopropanol. It has limited stability with aliphatic, aromatic and halogenated
hydrocarbons, THF, phenol and derivatives, concentrated acids and bases. For
normal phase applications, the maximum pressure should be limited to 200 bar.

Tantalum (Ta)
Tantalum is inert to most common HPLC solvents and almost all acids except
fluoric acid and acids with free sulfur trioxide. It can be corroded by strong bases
(e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use
with fluoric acid and fluorides.

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Solvent Information

Stainless Steel (SST)

Stainless steel is inert against many common solvents. It is stable in the
presence of acids and bases in a pH range of 1 to 12.5. It can be corroded by
acids below pH 2.3. It can also corrode in following solvents:
• Solutions of alkali halides, their respective acids (for example, lithium iodide,
potassium chloride, and so on) and aqueous solutions of halogens.
• High concentrations of inorganic acids like nitric acid, sulfuric acid and
organic solvents especially at higher temperatures (replace, if your
chromatography method allows, by phosphoric acid or phosphate buffer
which are less corrosive against stainless steel).
• Halogenated solvents or mixtures which form radicals and/or acids, for
example:
2 CHCl3 + O2→ 2 COCl2 + 2 HCl
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the stabilizing
alcohol.
• Chromatographic grade ethers, which can contain peroxides (for example,
THF, dioxane, diisopropylether). Such ethers should be filtered through dry
aluminium oxide which adsorbs the peroxides.
• Solutions of organic acids (acetic acid, formic acid, and so on) in organic
solvents. For example, a 1 % solution of acetic acid in methanol will attack
steel.
• Solutions containing strong complexing agents (for example, EDTA, ethylene
diamine tetra-acetic acid).
• Mixtures of carbon tetrachloride with isopropanol or THF.

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Solvent Information

Titanium (Ti)
Titanium is highly resistant to oxidizing acids (for example, nitric, perchloric and
hypochlorous acid) over a wide range of concentrations and temperatures. This
is due to a thin oxide layer on the surface, which is stabilized by oxidizing
compounds. Non-oxidizing acids (for example, hydrochloric, sulfuric and
phosphoric acid) can cause slight corrosion, which increases with acid
concentration and temperature. For example, the corrosion rate with 3 % HCl
(about pH 0.1) at room temperature is about 13 μm/year. At room temperature,
titanium is resistant to concentrations of about 5 % sulfuric acid (about pH 0.3).
Addition of nitric acid to hydrochloric or sulfuric acids significantly reduces
corrosion rates. Titanium is sensitive to acidic metal chlorides like FeCl3 or CuCl2.
Titanium is subject to corrosion in anhydrous methanol, which can be avoided by
adding a small amount of water (about 3 %). Slight corrosion is possible with
ammonia > 10 %.

Diamond-Like Carbon (DLC)

Diamond-Like Carbon is inert to almost all common acids, bases and solvents.
There are no documented incompatibilities for HPLC applications.

Fused silica and Quartz (SiO2)

Fused silica is used in Max Light Cartridges. Quartz is used for classical flow cell
windows. It is inert against all common solvents and acids except hydrofluoric
acid and acidic solvents containing fluorides. It is corroded by strong bases and
should not be used above pH 12 at room temperature. The corrosion of flow cell
windows can negatively affect measurement results. For a pH greater than 12,
the use of flow cells with sapphire windows is recommended.

Gold
Gold is inert to all common HPLC solvents, acids and bases within the specified
pH range. It can be corroded by complexing cyanides and concentrated acids like
aqua regia.

Zirconium Oxide (ZrO2)

Zirconium Oxide is inert to almost all common acids, bases and solvents. There
are no documented incompatibilities for HPLC applications.

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Solvent Information

Platinum/Iridium
Platinum/Iridium is inert to almost all common acids, bases and solvents. There
are no documented incompatibilities for HPLC applications.

Fluorinated polymers (PTFE, PFA, FEP, FFKM, PVDF)

Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy),
and FEP (fluorinated ethylene propylene) are inert to almost all common acids,
bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to
most chemicals. As an elastomer, it may swell in some organic solvents like
halogenated hydrocarbons.
TFE/PDD copolymer tubings, which are used in all Agilent degassers except
G1322A/G7122A, are not compatible with fluorinated solvents like Freon,
Fluorinert, or Vertrel. They have limited life time in the presence of
hexafluoroisopropanol (HFIP). To ensure the longest possible life with HFIP, it is
best to dedicate a particular chamber to this solvent, not to switch solvents, and
not to let dry out the chamber. For optimizing the life of the pressure sensor, do
not leave HFIP in the chamber when the unit is off.
The tubing of the leak sensor is made of PVDF (polyvinylidene fluoride), which is
incompatible with the solvent DMF (dimethyl formamide).

Sapphire, Ruby and Al2O3-based ceramics

Sapphire, ruby and ceramics based on aluminum oxide Al2O3 are inert to almost
all common acids, bases and solvents. There are no documented
incompatibilities for HPLC applications.

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Algae Growth in HPLC Systems

Algae Growth in HPLC Systems

The presence of algae in HPLC systems can cause a variety of problems that
may be incorrectly diagnosed as instrument or application problems. Algae grow
in aqueous media, preferably in a pH range of 4-8. Their growth is accelerated by
buffers, for example phosphate or acetate. Since algae grow through
photosynthesis, light will also stimulate their growth. Even in distilled water
small-sized algae grow after some time.

Instrumental Problems Associated With Algae

Algae deposit and grow everywhere within the HPLC system, causing the
followng problems:
• Blocked solvent filters, or deposits on inlet or outlet valves, resulting in
unstable flow, composition or gradient problems, or a complete failure of the
pump.
• Plugging of small-pore, high-pressure solvent filters, usually placed before the
injector, resulting in high system pressure.
• Blockage of PTFE frits, leading to increased system pressure.
• Plugging of column filters, giving high system pressure.
• Dirty flow cell windows of detectors, resulting in higher noise levels (since the
detector is the last module in the flow path, this problem is less common).

How to Prevent and/or Reduce the Algae Problem

• Always use freshly prepared solvents, especially use demineralized water
which was filtered through 0.2 µm filters.
• Never leave mobile phase in the instrument for several days without flow.
• Always discard old mobile phase.
• Use the amber solvent bottle (Solvent bottle, amber, 1000 mL (9301-6526))
supplied with the instrument for your aqueous mobile phase.
• If possible add a few mg/L sodium azide or a few percent organic solvent to
the aqueous mobile phase.

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3 Using the Pump
Prevent Blocking of Solvent Filters

Prevent Blocking of Solvent Filters

Contaminated solvents or algae growth in the solvent bottle will reduce the
lifetime of the solvent filter and will influence the performance of the module.
This is especially true for aqueous solvents or phosphate buffers (pH 4 to 7). The
following suggestions will prolong lifetime of the solvent filter and will maintain
the performance of the module.
• Use a sterile, if possible amber, solvent bottle to slow down algae growth.
• Filter solvents through filters or membranes that remove algae.
• Exchange solvents every two days or refilter.
• If the application permits add 0.0001 – 0.001 M sodium azide to the solvent.
• Place a layer of argon on top of your solvent.
• Avoid exposure of the solvent bottle to direct sunlight.

Never use the system without solvent filter installed.

NOTE

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3 Using the Pump
Prevent Blocking of Solvent Filters

Checking the Solvent Filters

The solvent filters are located on the low-pressure side of the binary pump. A
blocked filter therefore does not necessarily affect the high pressure readings of
the pump. The pressure readings cannot be used to check whether the filters are
blocked or not. If the solvent cabinet is placed on top of the binary pump, the filter
condition can be checked in the following way:
Remove the solvent inlet tube from the inlet port of the solvent selection valve or
the degasser. If the filter is in good condition, the solvent will freely drip out of the
solvent tube (due to hydrostatic pressure). If the solvent filter is partly blocked
only very little solvent will drip out of the solvent tube.

When opening capillary or tube fittings, solvents may leak out.

WAR N IN G
The handling of toxic and hazardous solvents and reagents can carry
health risks.
 Observe appropriate safety procedures (for example, wear goggles,
safety gloves and protective clothing) as described in the material
handling and safety data sheet supplied by the solvent vendor,
especially when toxic or hazardous solvents are used.

Cleaning the Solvent Filters

• Remove the blocked solvent filter from the bottle-head assembly and place it
in a beaker with concentrated nitric acid (35%) for one hour.
• Thoroughly flush the filter with HPLC-grade water (remove all nitric acid,
some capillary columns can be damaged by nitric acid).
• Replace the filter.

Never use the system without solvent filter installed.

NOTE

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3 Using the Pump
Normal Phase Applications

Normal Phase Applications

Current passive inlet valves and outlet ball valves used with 1260 and 1290
Infinity pumps do not work well with applications using non-polar solvents as for
normal phase applications (e.g. hexane, heptane and CO2). With such
applications, pressure drops could be observed. They are a result of particles
electrostatically charging up in insulating solvents and sticking to the balls inside
the valves, such that the valves do not close properly any more after some time
of use (can be hours).
For normal phase applications, a second type of valves is available, which has a
design based on the existing one for 1260 and 1290 Infinity valves. These valves
use a new material for valve balls, which is a conductive ceramic and replaces
non-conductive ruby balls. The balls do not charge up electrostatically and show
good performance in normal phase.
The valves are marked with N for non-polar or normal phase.
Agilent recommends using these valves for (and only for) normal phase
applications.
No design change has been done for active inlet valves, which have already been
used successfully in the past for normal phase applications in 1200 Series and
1260 Infinity binary pumps.

Corrosion of valves
C AU T I O N
Normal phase balls/valves corrode quickly in aqueous solutions and acids (at
or below pH 7).
 Do not use normal phase valves in applications running with aqueous
solutions.

The N-Valves have been tested successfully in using hexane at pressures below
100 bar; heptane can be used as a substitute for neurotoxic hexane.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 57

3 Using the Pump
Normal Phase Applications

Seals for Normal Phase Applications

For running normal phase applications on 1200 Infinity Series pumps, yellow PE
seals are required, which exist as piston seals and wash seals. Seal wash is very
uncommon for normal phase applications (no buffers needed), but wash seals
are needed for seal wash pump heads.
1290 Infinity pumps use PE seals by default. In combination with ceramic
pistons, PE seals are used for both reversed phase (1200 bar) and normal phase
applications.
1260 Infinity pumps use sapphire pistons and black PTFE piston and wash seals
by default (600 bar). Such PTFE seals create small wear particles in normal
phase applications, which can clog valves and other parts in the flow path.
PE seals have a limited life time when used with normal phase solvents and
sapphire pistons. Agilent recommends a maximum pressure of 200 bar for this
combination, which shall also be applied for pressure tests.

Choice of Normal Phase Valves and Seals

Table 7 Recommended valves and seals for normal phase applications

1260 Infinity 1290 Infinity

Inlet valves 1260 Infinity Inlet Valve Type N 1290 Infinity Inlet Valve Type N (G4220-60122)
(G1312-60166) 1290 Infinity Quat Inlet Valve Type N
(G4204-60122)

Outlet valves Outlet Valve Type N/SFC 1290 Infinity Outlet Valve Type N (G4220-60128)
(G1312-60167)

Seals PE seals (pack of 2) (0905-1420)

Wash Seal PE (0905-1718)

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 58

4 Optimizing Performance

When to Use a Vacuum Degasser 60

Operational Hints for the Vacuum Degasser 60
When to Use the Active Seal Wash Option 61
When to Use the Low Volume Mixer 62
When to Remove Damper and Mixer 63
Convert the Binary Pump to Low Delay Volume Mode 64
How to Optimize the Compressibility Compensation Setting 65
Solvent Compressibility Calibration 65
Optimization of Legacy Compressibility Settings 66

This chapter gives information on how to optimize the performance of the Binary
Pump under special operational conditions.

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4 Optimizing Performance
When to Use a Vacuum Degasser

When to Use a Vacuum Degasser

A degasser removes air, which is dissolved in any solvent. When solvents are
heated or mixed with other solvents, air can leave the solvent and form small
bubbles. Over time, these bubbles accumulate and can cause pressure
fluctuations which may finally result in retention time shifts.
All Agilent 1200 Infinity II Series Pumps have a built-in degasser. While a
degasser is needed for low pressure mixing pumps like Agilent quaternary
pumps, high pressure mixing pumps like Agilent binary pumps are more robust
with respect to bubble formation. However, a degasser is recommended for best
performance.
Additionally, a degasser is highly recommended for the following applications:
• Your detector is used with maximum sensitivity in the low UV wavelength
range.
• Your application requires highest injection precision.
• Your application requires highest retention-time reproducibility (flow rates
below 0.5 mL/min).
• The binary pump is used with bypassed damper and mixer.
The external 1260 Infinity II Degasser G7122A is recommended for use with
applications using highly volatile solvents like Hexane or DCM, solvents with
special characteristics like THF, or applications using refractive index detection.

Operational Hints for the Vacuum Degasser

If you are using the vacuum degasser for the first time, if the vacuum degasser
was switched off for any length of time (for example, overnight), or if the vacuum
degasser chambers are empty, you have to prime the vacuum degasser before
running an analysis. Priming is usually done by pumping at a high flow rate
(3 – 5 mL/min). Alternatively, a syringe can be used to draw the solvent through
the (empty) degasser if the pump does not aspirate the solvent by itself.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 60

4 Optimizing Performance
When to Use the Active Seal Wash Option

When to Use the Active Seal Wash Option

Concentrated buffer solutions will reduce the lifetime of the seals and pistons in
your binary pump. The active seal wash option allows to maintain the seal
lifetime by flushing the low pressure side of the seals with a wash solvent.
The seal wash option is strongly recommended if buffer concentrations of 0.1 M
or higher are used regularly with the pump.
The active seal wash option kit can be ordered by quoting Active Seal Wash
Upgrade Product including Service (G1399A).
The seal wash option comprises a peristaltic pump, secondary seals, gaskets,
seal holders and tubing for both pump heads. A bottle of premixed
water/isopropanol (90/10 vol%) is placed in the solvent cabinet and connected to
the peristaltic pump.
Always use a mixture of HPLC-grade water (90 %) and isopropanol (10 %) as
wash solvent. This mixture prevents bacteria growth in the wash bottle and
reduces the surface tension of the water.

In order to avoid accumulation of buffer salts or impurities, regularly replace the

NOTE washing solution using fresh solvents.

The operation of the peristaltic pump can be controlled from the data system or
the Instant Pilot.
For adding a seal-wash option, please contact your local Agilent Technologies
service representative.

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4 Optimizing Performance
When to Use the Low Volume Mixer

When to Use the Low Volume Mixer

The Low volume mixer ( 200 µL) (5067-1565) is designed for use with the Agilent
InfinityLab LC Series 1260 Infinity II Binary LC System in low delay volume mode.
This configuration is typically used for 2.1 mm i.d., 1.8 µm particle size columns,
where emphasis is put on S/N ratio. The low volume mixer helps mixing
gradients starting with a low concentration of organic solvents, which can cause
noise on the baseline.

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4 Optimizing Performance
When to Remove Damper and Mixer

When to Remove Damper and Mixer

The binary pump is equipped with a pressure pulsation damper and a static
mixer. The total delay volume of the pump is 600 – 800 µL (depending on system
pressure). The mixer has a volume of 400 µL.
For applications that require lowest delay volume (for example, fast gradient
methods or gradient applications with low flow rates), damper and mixer can be
bypassed.

Standard configuration Low delay volume configuration

Figure 9 Flow path modifications of the Binary Pump

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4 Optimizing Performance
When to Remove Damper and Mixer

Convert the Binary Pump to Low Delay Volume Mode

The binary pump is delivered in standard configuration (damper and mixer
connected). This paragraph shows how to bypass damper and mixer and convert
the pump to low delay volume mode.
Configurations where only damper or mixer are disconnected while the other part
is still in line are not supported by Agilent Technologies.

Tools required p/n Description

8710-0510 A Wrench, 1/4 - 5/16 inch, open

Wrench, 14 mm
Hex driver open, 1/4 inch

Preparations Flush the system (water if buffers were used, otherwise isopropanol).
Turn the flow off.

1 Open the doors. 2 Use the 1/4 inch hex driver to remove fitting B from port
2 of the pressure sensor. Fold capillary end B away. It
remains unconnected.

2
B

3 Disconnect fitting A from outlet 1 of the mixer. 4 Connect fitting A to port 2 of the pressure sensor. Seal
port 1 of the mixer with a plastic blank nut.

A
1 A 1
2

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 64

4 Optimizing Performance
How to Optimize the Compressibility Compensation Setting

How to Optimize the Compressibility

Compensation Setting

When a solvent is metered at ambient pressure and compressed to a higher

pressure, the volume decreases depending on its compressibility. Solvent
compressibility is a non-linear function of pressure and temperature. It is specific
for each solvent.
In order to deliver the desired flow accurately at all pressures, Agilent pumps use
a compressibility compensation. For standard LC applications, e.g. using a
400 bar binary pump, an average compressibility value for the solvent is
sufficient.
For the 600 bar 1260 Infinity II Binary Pump , the pressure-dependency of a
solvent compressibility needs to be considered. It is determined at different
pressures between 0 – 600 bar. The pump uses the obtained non-linear function
to select the correct compressibility value for the actual pump pressure.
Compressibility data for the most common solvents is readily available in the
pump firmware.
The compensation algorithm is so powerful that the damper and mixer can be
removed from the pump flow path at low flow rate while the pressure ripple and
composition ripple remain at low levels.
For method compatibility reasons, the legacy compressibility compensation is
still available.

Solvent Compressibility Calibration

Unlisted or premixed solvents can be calibrated with the Solvent Compressibility
Calibration function. For a detailed description, see “Binary Pump Solvent
Compressibility Calibration” on page 100.

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4 Optimizing Performance
How to Optimize the Compressibility Compensation Setting

Optimization of Legacy Compressibility Settings

The compressibility compensation default settings are 50 × 10-6 /bar (best for
most aqueous solutions) for pump head A and 115 × 10-6 /bar (to suit organic
solvents) for pump head B. The settings represent average values for aqueous
solvents (A side) and organic solvents (B side). Therefore it is always
recommended to use the aqueous solvent on the A side of the pump and the
organic solvent on the B side. Under normal conditions, the default settings
reduce the pressure pulsation to below 2 % of system pressure, which is
sufficient for most applications. If the compressibility values for the solvents
used differ from the default settings, it is recommended to change the
compressibility values accordingly. Compressibility settings can be optimized by
using the values for various solvents described in Table 8 on page 67. If the
solvent in use is not listed in the compressibility table, when using premixed
solvents and if the default settings are not sufficient for your application, the
following procedure can be used to optimize the compressibility settings:
1 Start channel A of the binary pump with the required flow rate.
2 Before starting the optimization procedure, the flow must be stable. Use
degassed solvent only. Check the tightness of the system with the pressure
test (see “(System) Pressure Test” on page 93).
3 Your pump must be connected to an Agilent data system or Instant Pilot, the
pressure- and %-ripple can be monitored with one of these instruments.
4 Start the recording device in plot mode.
5 Starting with a compressibility setting of 40 × 10-6 /bar, increase the value in
steps of 10. The compressibility compensation setting that generates the
smallest pressure ripple is the optimum value for your solvent composition.
6 Repeat step 1 through step 5 for the B channel of your binary pump.

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4 Optimizing Performance
How to Optimize the Compressibility Compensation Setting

Table 8 Solvent Compressibility

Solvent (pure) Compressibility (10-6/bar)

Acetone 126

Acetonitrile 115

Benzene 95

Carbon tetrachloride 110

Chloroform 100

Cyclohexane 118

Ethanol 114

Ethyl acetate 104

Heptane 120

Hexane 150

Isobutanol 100

Isopropanol 100

Methanol 120

1-Propanol 100

Toluene 87

Water 46

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 67

5 Troubleshooting and Diagnostics

Overview of the Module’s Indicators and Test Functions 69

User Interfaces 71
Agilent Lab Advisor Software 72

Overview of the troubleshooting and diagnostic features.

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5 Troubleshooting and Diagnostics
Overview of the Module’s Indicators and Test Functions

Overview of the Module’s Indicators and Test

Functions

Status Indicators
The module is provided with two status indicators which indicate the operational
state of the module. The status indicators provide a quick visual check of the
operation of the module.

Error Messages
In the event of an electronic, mechanical or hydraulic failure, the module
generates an error message in the user interface. For each message, a short
description of the failure, a list of probable causes of the problem, and a list of
suggested actions to fix the problem are provided (see chapter Error
Information).

Test Functions
A series of test functions are available for troubleshooting and operational
verification after exchanging internal components (see Tests and Calibrations).

Pressure Test
The Pressure Test is a quick test designed to determine the pressure tightness of
the system (i.e. the high pressure flow path between pump and column). After
exchanging flow path components (e.g. pump seals or injection seal), use this
test to verify the system is pressure tight, see “(System) Pressure Test” on
page 93.

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5 Troubleshooting and Diagnostics
Overview of the Module’s Indicators and Test Functions

Solvent Compressibility Calibration

Solvent compressibility is a function of solvent type and pressure. In order to
optimize flow accuracy and pressure ripple, the compressibility of the solvent
must be considered. The binary pump firmware contains compressibility
parameters for most commonly used solvents. A compressibility calibration
function is available to generate compressibility data for unlisted solvents (see
“Binary Pump Solvent Compressibility Calibration” on page 100). The
compressibility data are stored in an XML file and can be transferred to other
InfinityLab LC Series binary pumps.

Pump Elasticity Calibration

Various parts in the flow path of the binary pump have a certain elasticity which
needs to be compensated to obtain the lowest pressure-, flow- and composition
ripple possible. This is done by running an elasticity calibration after maintenance
and major repairs. For details see “Pump Elasticity Calibration” on page 102.

Pump Leak Rate Test

The Pump Leak Rate Test is a diagnostic test designed to determine the pressure
tightness of the pump components. When a problem with the pump is
suspected, use this test to help troubleshoot the pump and its pumping
performance, see “Pump Leak Rate Test” on page 104.

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5 Troubleshooting and Diagnostics
User Interfaces

User Interfaces

Depending on the user interface, the available tests vary. Some descriptions are
only available in the Service Manual.

Test Instant Pilot G4208A Agilent Lab Advisor

Pressure Test Yes Yes

Valve Test No Yes

Solvent compressibility No Yes

calibration

Pump elasticity calibration No Yes

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 71

5 Troubleshooting and Diagnostics
Agilent Lab Advisor Software

Agilent Lab Advisor Software

The Agilent Lab Advisor Software (basic license, shipped with an Agilent LC
pump) is a standalone product that can be used with or without a
chromatographic data system. Agilent Lab Advisor helps to manage the lab for
high-quality chromatographic results by providing a detailed system overview of
all connected analytical instruments with instrument status, Early Maintenance
Feedback counters (EMF), instrument configuration information, and diagnostic
tests. With the push of a button, a detailed diagnostic report can be generated.
Upon request, the user can send this report to Agilent for a significantly improved
troubleshooting and repair process.
The Agilent Lab Advisor software is available in two versions:
• Lab Advisor Basic
• Lab Advisor Advanced
Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Agilent
InfinityLab LC Series instrument.
The Lab Advisor Advanced features can be unlocked by purchasing a license key,
and include real-time monitoring of instrument actuals, all various instrument
signals, and state machines. In addition, all diagnostic test results, calibration
results, and acquired signal data can be uploaded to a shared network folder. The
Review Client included in Lab Advisor Advanced allows to load and examine the
uploaded data no matter on which instrument it was generated. This makes Data
Sharing an ideal tool for internal support groups and users who want to track the
instrument history of their analytical systems.
The optional Agilent Maintenance Wizard Add-on provides an easy-to-use,
step-by-step multimedia guide for performing preventive maintenance on Agilent
1200 Infinity LC Series instrument.
The tests and diagnostic features that are provided by the Agilent Lab Advisor
software may differ from the descriptions in this manual. For details, refer to the
Agilent Lab Advisor software help files.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 72

6 Error Information

What Are Error Messages 74

General Error Messages 75
Timeout 75
Shutdown 75
Remote Timeout 76
Lost CAN Partner 76
Leak 77
Leak Sensor Open 78
Leak Sensor Short 78
Compensation Sensor Open 79
Compensation Sensor Short 79
Fan Failed 80
Module Error Messages 81
Solvent Zero Counter 81
Pressure Above Upper Limit 81
Pressure Below Lower Limit 82
Pressure Signal Missing 82
Valve Failed 83
Electronic Fuse of SSV Open 84
AIV Fuse 84
Motor-Drive Power 85
Encoder Missing 86
Servo Restart Failed 87
Pump Head Missing 88
Index Limit 88
Index Adjustment 89
Index Missing 89
Initialization Failed 90
Degasser: signal fail 90
Degasser: Vacuum cannot be maintained 91
Degasser: limit not reached 91

This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.

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6 Error Information
What Are Error Messages

What Are Error Messages

Error messages are displayed in the user interface when an electronic,

mechanical, or hydraulic (flow path) failure occurs which requires attention
before the analysis can be continued (for example, repair, or exchange of
consumables is necessary). In the event of such a failure, the red status indicator
at the front of the module is switched on, and an entry is written into the module
logbook.
If an error occurs outside a method run, other modules will not be informed about
this error. If it occurs within a method run, all connected modules will get a
notification, all LEDs get red and the run will be stopped. Depending on the
module type, this stop is implemented differently. For example, for a pump the
flow will be stopped for safety reasons. For a detector, the lamp will stay on in
order to avoid equilibration time. Depending on the error type, the next run can
only be started, if the error has been resolved, for example liquid from a leak has
been dried. Errors for presumably single time events can be recovered by
switching on the system in the user interface.
Special handling is done in case of a leak. As a leak is a potential safety issue and
may have occurred at a different module from where it has been observed, a leak
always causes a shutdown of all modules, even outside a method run.
In all cases, error propagation is done via the CAN bus or via an APG/ERI remote
cable (see documentation for the APG/ERI interface).

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6 Error Information
General Error Messages

General Error Messages

Timeout
Error ID: 0062
The timeout threshold was exceeded.

Probable cause Suggested actions

1 The analysis was completed successfully, Check the logbook for the occurrence and source
and the timeout function switched off the of a not-ready condition. Restart the analysis
module as requested. where required.

2 A not-ready condition was present during a Check the logbook for the occurrence and source
sequence or multiple-injection run for a of a not-ready condition. Restart the analysis
period longer than the timeout threshold. where required.

Shutdown
Error ID: 0063
An external instrument has generated a shutdown signal on the remote line.
The module continually monitors the remote input connectors for status signals.
A LOW signal input on pin 6 of the Enhanced Remote Interface (ERI) connector
generates the error message.

Probable cause Suggested actions

1 Leak detected in another module with a CAN Fix the leak in the external instrument before
connection to the system. restarting the module.

2 Leak detected in an external instrument with Fix the leak in the external instrument before
a remote connection to the system. restarting the module.

3 Shut-down in an external instrument with a Check external instruments for a shut-down

remote connection to the system. condition.

4 The degasser failed to generate sufficient Check the vacuum degasser for an error
vacuum for solvent degassing. condition. Refer to the Service Manual for the
degasser or the pump that has the degasser
built-in.

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6 Error Information
General Error Messages

Remote Timeout
Error ID: 0070
A not-ready condition is still present on the remote input. When an analysis is
started, the system expects all not-ready conditions (for example, a not-ready
condition during detector balance) to switch to run conditions within one minute
of starting the analysis. If a not-ready condition is still present on the remote line
after one minute the error message is generated.

Probable cause Suggested actions

1 Not-ready condition in one of the Ensure the instrument showing the not-ready
instruments connected to the remote line. condition is installed correctly, and is set up
correctly for analysis.

2 Defective remote cable. Exchange the remote cable.

3 Defective components in the instrument Check the instrument for defects (refer to the
showing the not-ready condition. instrument’s documentation).

Lost CAN Partner

Error ID: 0071
During an analysis, the internal synchronization or communication between one
or more of the modules in the system has failed.
The system processors continually monitor the system configuration. If one or
more of the modules is no longer recognized as being connected to the system,
the error message is generated.

Probable cause Suggested actions

1 CAN cable disconnected. • Ensure all the CAN cables are connected
correctly.
• Ensure all CAN cables are installed correctly.

2 Defective CAN cable. Exchange the CAN cable.

3 Defective mainboard in another module. Switch off the system. Restart the system, and
determine which module or modules are not
recognized by the system.

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6 Error Information
General Error Messages

Leak
Error ID: 0064
A leak was detected in the module.
The signals from the two temperature sensors (leak sensor and board-mounted
temperature-compensation sensor) are used by the leak algorithm to determine
whether a leak is present. When a leak occurs, the leak sensor is cooled by the
solvent. This changes the resistance of the leak sensor which is sensed by the
leak sensor circuit on the main board.

Probable cause Suggested actions

1 Loose fittings. Ensure all fittings are tight.

2 Broken capillary. Exchange defective capillaries.

3 Loose or leaking purge valve, inlet valve, or Ensure pump components are seated correctly.
outlet valve. If there are still signs of a leak, exchange the
appropriate seal (purge valve, inlet valve, outlet
valve).

4 Loose or leaking purge valve, active inlet Ensure pump components are seated correctly.
valve, or outlet valve. If there are still signs of a leak, exchange the
appropriate seal (purge valve, active inlet valve,
outlet valve).

5 Defective pump seals. Exchange the pump seals.

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6 Error Information
General Error Messages

Leak Sensor Open

Error ID: 0083
The leak sensor in the module has failed (open circuit).
The current through the leak sensor is dependent on temperature. A leak is
detected when solvent cools the leak sensor, causing the leak sensor current to
change within defined limits. If the current falls outside the lower limit, the error
message is generated.

Probable cause Suggested actions

1 Leak sensor not connected to the power Please contact your Agilent service
switch board. representative.

2 Defective leak sensor. Please contact your Agilent service

representative.

3 Leak sensor incorrectly routed, being Please contact your Agilent service
pinched by a metal component. representative.

4 Power switch assembly defective Please contact your Agilent service

representative.

Leak Sensor Short

Error ID: 0082
The leak sensor in the module has failed (short circuit).
The current through the leak sensor is dependent on temperature. A leak is
detected when solvent cools the leak sensor, causing the leak sensor current to
change within defined limits. If the current increases above the upper limit, the
error message is generated.

Probable cause Suggested actions

1 Defective leak sensor. Please contact your Agilent service

representative.

2 Leak sensor incorrectly routed, being Please contact your Agilent service
pinched by a metal component. representative.

3 Power switch assembly defective Please contact your Agilent service

representative.

4 Cable or contact problem. Please contact your Agilent service

representative.

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6 Error Information
General Error Messages

Compensation Sensor Open

Error ID: 0081
The ambient-compensation sensor (NTC) on the power switch board in the
module has failed (open circuit).
The resistance across the temperature compensation sensor (NTC) on the
power switch board is dependent on ambient temperature. The change in
resistance is used by the leak circuit to compensate for ambient temperature
changes. If the resistance across the sensor increases above the upper limit, the
error message is generated.

Probable cause Suggested actions

1 Loose connection between the power switch Please contact your Agilent service
board and the mainboard representative.

2 Defective power switch assembly Please contact your Agilent service

representative.

Compensation Sensor Short

Error ID: 0080
The ambient-compensation sensor (NTC) on the power switch board in the
module has failed (open circuit).
The resistance across the temperature compensation sensor (NTC) on the
power switch board is dependent on ambient temperature. The change in
resistance is used by the leak circuit to compensate for ambient temperature
changes. If the resistance across the sensor falls below the lower limit, the error
message is generated.

Probable cause Suggested actions

1 Defective power switch assembly Please contact your Agilent service

representative.

2 Loose connection between the power switch Please contact your Agilent service
board and the mainboard representative.

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6 Error Information
General Error Messages

Fan Failed
Error ID: 0068
The cooling fan in the module has failed.
The hall sensor on the fan shaft is used by the mainboard to monitor the fan
speed. If the fan speed falls below a certain limit for a certain length of time, the
error message is generated.
This limit is given by 2 revolutions/second for longer than 5 seconds.
Depending on the module, assemblies (e.g. the lamp in the detector) are turned
off to assure that the module does not overheat inside.

Probable cause Suggested actions

1 Fan cable disconnected. Please contact your Agilent service

representative.

2 Defective fan. Please contact your Agilent service

representative.

3 Defective mainboard. Please contact your Agilent service

representative.

4 Improperly positioned cables or wires Please contact your Agilent service

obstructing fan blades. representative.

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6 Error Information
Module Error Messages

Module Error Messages

Solvent Zero Counter

Error ID: 2055
The error message is triggered if the remaining volume in a solvent bottle falls
below the set limit.

Probable cause Suggested actions

1 Volume in bottle below specified volume. Refill bottles and reset solvent counters.

2 Incorrect setting. Make sure the set solvent volume matches the
actual bottle filling and set the shutoff limit to a
reasonable value (e.g. 100 mL for 1 L bottles)

Pressure Above Upper Limit

Error ID: 2014, 2500
The system pressure has exceeded the upper pressure limit.

Probable cause Suggested actions

1 Upper pressure limit set too low. Ensure the upper pressure limit is set to a value
suitable for the analysis.

2 Blockage in the flowpath (after the pressure Check for blockage in the flow path. The
sensor). following components are particularly subject to
blockage: inline filter frit, needle (autosampler),
seat capillary (autosampler), sample loop
(autosampler), column frits and capillaries with
small internal diameters (e.g. 50 µm ID).

3 Defective pressure sensor. Please contact your Agilent service

representative.

4 Defective mainboard. Please contact your Agilent service

representative.

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6 Error Information
Module Error Messages

Pressure Below Lower Limit

Error ID: 2015, 2501
The system pressure has fallen below the lower pressure limit.

Probable cause Suggested actions

1 Lower pressure limit set too high. Ensure the lower pressure limit is set to a value
suitable for the analysis.

2 Air bubbles in the mobile phase. • Make sure that the degasser is in flow path
and works correctly. Purge the module.
• Ensure solvent inlet filters are not blocked.

3 Leak. • Inspect the pump head, capillaries and

fittings for signs of a leak.
• Purge the module. Run a pressure test to
determine whether the seals or other module
components are defective.

4 Defective pressure sensor. Please contact your Agilent service

representative.

5 Defective mainboard. Please contact your Agilent service

representative.

Pressure Signal Missing

Error ID: 2016
The pressure signal is missing.
The pressure signal must be within a specific voltage range. If the pressure signal
is missing, the processor detects a voltage of approximately -120 mV across the
pressure sensor.

Probable cause Suggested actions

1 Pressure sensor disconnected Please contact your Agilent service

representative.

2 Defective pressure sensor Please contact your Agilent service

representative.

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6 Error Information
Module Error Messages

Valve Failed
Error ID: 2040
Valve 0 Failed: valve A1
Valve 1 Failed: valve A2
Valve 2 Failed: valve B2
Valve 3 Failed: valve B1
One of the solvent selection valves in the module failed to switch correctly.
The processor monitors the valve voltage before and after each switching cycle.
If the voltages are outside expected limits, the error message is generated.

Probable cause Suggested actions

1 Solvent selection valve disconnected. Please contact your Agilent service

representative.

2 Connection cable (inside instrument) not Please contact your Agilent service
connected. representative.

3 Connection cable (inside instrument) Please contact your Agilent service

defective. representative.

4 Solvent selection valve defective. Exchange the solvent selection valve.

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6 Error Information
Module Error Messages

Electronic Fuse of SSV Open

Error ID: 2049
Valve Fuse 0: Channels A1 and A2
Valve Fuse 1: Channels B1 and B2
One of the solvent-selection valves in the module has drawn excessive current
causing the selection-valve electronic fuse to open.

Probable cause Suggested actions

1 Defective solvent selection valve. Restart the pump. If the error message appears
again, exchange the solvent selection valve.

2 Defective connection cable (front panel to Please contact your Agilent service
main board). representative.

3 Defective mainboard. Please contact your Agilent service

representative.

AIV Fuse
Error ID: 2044
Inlet-Valve Fuse 0: Pump channel A
Inlet-Valve Fuse 1: Pump channel B
One of the active-inlet valves in the module has drawn excessive current causing
the inlet-valve electronic fuse to open.

Probable cause Suggested actions

1 Defective active inlet valve. Restart the module. If the error message appears
again, exchange the active inlet valve.

2 Defective connection cable (front panel to Please contact your Agilent service
main board). representative.

3 Defective mainboard. Please contact your Agilent service

representative.

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6 Error Information
Module Error Messages

Motor-Drive Power
Error ID: 2041, 2042
Motor-Drive Power: Pump channel A
B: Motor-Drive Power: Pump channel B
The current drawn by the pump motor exceeded the maximum limit.
Blockages in the flow path are usually detected by the pressure sensor, which
result in the pump switching off when the upper pressure limit is exceeded. If a
blockage occurs before the pressure sensor, the pressure increase cannot be
detected and the module will continue to pump. As pressure increases, the pump
drive draws more current. When the current reaches the maximum limit, the
module is switched off, and the error message is generated.

Probable cause Suggested actions

1 Flow path blockage in front of the pressure Ensure the capillaries and frits between the
sensor. pump head and pressure sensor inlet are free
from blockage.

2 Blocked (passive or active) inlet valve. Exchange the (passive or active) inlet valve.

3 Blocked outlet valve. Exchange the outlet valve.

4 High friction (partial mechanical blockage) in Remove the pump-head assembly. Ensure there
the pump drive assembly. is no mechanical blockage of the pump-head
assembly or pump drive assembly.

5 Defective pump drive assembly. Please contact your Agilent service

representative.

6 Defective mainboard. Please contact your Agilent service

representative.

7 Restriction capillary blocked at pre-mixing Exchange restriction capillary.

union.

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6 Error Information
Module Error Messages

Encoder Missing
Error ID: 2046, 2050, 2510
Encoder Missing: Pump channel A
B: Encoder Missing: Pump channel B
The optical encoder on the pump motor in the module is missing or defective.
The processor checks the presence of the pump encoder connector every 2 s. If
the connector is not detected by the processor, the error message is generated.

Probable cause Suggested actions

1 Defective or disconnected pump encoder Please contact your Agilent service

connector. representative.

2 Defective pump drive assembly. Please contact your Agilent service

representative.

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6 Error Information
Module Error Messages

Servo Restart Failed

Error ID: 2201, 2211
Servo Restart Failed: Pump channel A
B: Servo Restart Failed: Pump channel B
The pump motor in the module was unable to move into the correct position for
restarting.
When the module is switched on, the first step is to switch on the C phase of the
variable reluctance motor. The rotor should move to one of the C positions. The C
position is required for the servo to be able to take control of the phase
sequencing with the commutator. If the rotor is unable to move, or if the C
position cannot be reached, the error message is generated.

Probable cause Suggested actions

1 Mechanical blockage of the module. Remove the pump-head assembly. Ensure there
is no mechanical blockage of the pump-head
assembly or pump drive assembly.

2 Disconnected or defective cable. Please contact your Agilent service

representative.

3 Blocked (passive or active) inlet valve. Exchange the (passive or active) inlet valve.

4 Defective pump drive assembly. Please contact your Agilent service

representative.

5 Defective mainboard. Please contact your Agilent service

representative.

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6 Error Information
Module Error Messages

Pump Head Missing

Error ID: 2202, 2212
Pump Head Missing: Pump channel A
B: Pump Head Missing: Pump channel B
The pump-head end stop in the pump was not found.
When the pump restarts, the metering drive moves forward to the mechanical
end stop. Normally, the end stop is reached within 20 s, indicated by an increase
in motor current. If the end point is not found within 20 s, the error message is
generated.

Probable cause Suggested actions

1 Pump head not installed correctly (screws Install the pump head correctly. Ensure nothing
not secured, or pump head not seated (e.g. capillary) is trapped between the pump head
correctly). and body.

2 Broken piston. Exchange the piston.

Index Limit
Error ID: 2203, 2213
Index Limit: Pump channel A
B: Index Limit: Pump channel B
The time required by the piston to reach the encoder index position was too short
(pump).
During initialization, the first piston is moved to the mechanical stop. After
reaching the mechanical stop, the piston reverses direction until the encoder
index position is reached. If the index position is reached too fast, the error
message is generated.

Probable cause Suggested actions

1 Irregular or sticking drive movement. Remove the pump head, and examine the seals,
pistons, and internal components for signs of
wear, contamination or damage. Exchange
components as required.

2 Defective pump drive assembly. Please contact your Agilent service

representative.

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6 Error Information
Module Error Messages

Index Adjustment
Error ID: 2204, 2214
Index Adjustment: Pump channel A
B: Index Adjustment: Pump channel B
The encoder index position in the module is out of adjustment.
During initialization, the first piston is moved to the mechanical stop. After
reaching the mechanical stop, the piston reverses direction until the encoder
index position is reached. If the time to reach the index position is too long, the
error message is generated.

Probable cause Suggested actions

1 Irregular or sticking drive movement. Remove the pump head, and examine the seals,
pistons, and internal components for signs of
wear, contamination or damage. Exchange
components as required.

2 Defective pump drive assembly. Please contact your Agilent service

representative.

Index Missing
Error ID: 2205, 2215, 2505
Index Missing: Pump channel A
B: Index Missing: Pump channel B
The encoder index position in the module was not found during initialization.
During initialization, the first piston is moved to the mechanical stop. After
reaching the mechanical stop, the piston reverses direction until the encoder
index position is reached. If the index position is not recognized within a defined
time, the error message is generated.

Probable cause Suggested actions

1 Disconnected or defective encoder cable. Please contact your Agilent service

representative.

2 Defective pump drive assembly. Please contact your Agilent service

representative.

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6 Error Information
Module Error Messages

Initialization Failed
Error ID: 2207, 2217
Initialization Failed: Pump channel A
B: Initialization Failed: Pump channel B
The module failed to initialize successfully within the maximum time window.
A maximum time is assigned for the complete pump-initialization cycle. If the
time is exceeded before initialization is complete, the error message is
generated.

Probable cause Suggested actions

1 Blocked (passive or active) inlet valve. Exchange the (passive or active) inlet valve.

2 Defective pump drive assembly. Please contact your Agilent service

representative.

3 Defective mainboard. Please contact your Agilent service

representative.

Degasser: signal fail

Error ID: 2243, 8016
The pump board gets no or wrong pressure signals from the built-in degasser:
• No valid pressure signal is shown during startup of the degasser
• Measured pressure is higher than 32000 hPa

Probable cause Suggested actions

1 Degasser sensor defect Please contact your Agilent service

representative.

2 Degasser sensor not connected to Please contact your Agilent service

mainboard. representative.

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6 Error Information
Module Error Messages

Degasser: Vacuum cannot be maintained

Error ID: 8014
Pressure in degasser vacuum chamber exceeds 180 mbar limit during normal
operation.

Probable cause Suggested actions

1 Liquid in degasser tubing. Please contact your Agilent service

representative.

2 Leak in degasser tubing or chamber. Please contact your Agilent service

representative.

3 Degasser vacuum pump defective. Please contact your Agilent service

representative.

Degasser: limit not reached

Error ID: 2244, 8053
Degasser is not ready within 8 min after startup, because the pressure inside the
vacuum chamber exceeds 120 mbar.

Probable cause Suggested actions

1 Liquid in degasser tubing. Please contact your Agilent service

representative.

2 Leak in degasser tubing or chamber. Please contact your Agilent service

representative.

3 Degasser vacuum pump defect. Please contact your Agilent service

representative.

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7 Test Functions and Calibration

(System) Pressure Test 93

Positioning the Blank Nut 94
Running the System Pressure Test 95
Evaluating the Results 95
Valve Test 98
Running the Valve Test 99
Evaluating the Results 99
Binary Pump Solvent Compressibility Calibration 100
Running the Solvent Compressibility Calibration 101
Pump Elasticity Calibration 102
Running the Pump Elasticity Calibration 103
Pump Leak Rate Test 104
Running the Test 104
Evaluating the Results 105
Potential Causes of Leak Rate Test Failure 106

This chapter explains all test functions that are available for the binary pump.

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7 Test Functions and Calibration
(System) Pressure Test

(System) Pressure Test

This Lab Advisor test uses different names depending on the firmware revision
NOTE used: FW revision > A.06.50: System Pressure Test

Description
The system pressure test is a quick built-in test designed to demonstrate the leak
tightness of the system. The test involves monitoring the flow profile while the
pump delivers against a blank nut. The result is presented as the leak rate of the
module and provides information about the leak tightness of the system between
the outlet valves of the pump and the blank nut.

The blank nut can be positioned anywhere between the purge valve of the pump
NOTE and the detector inlet to pressure test the desired part of the system.

Blank nut placed at the outlet of flow cell

C AU T I O N
The applied pressure may cause permanent leaks or bursting of the flow cell.
 Never include the flow cell in the pressure test.

Step 1
The test begins with the initialization of both pump heads. After initialization, the
pump is starting the compression phase and the required flow rate is constantly
monitored and adjusted. The pump continues to pump until a system pressure of
around 600 bar is reached.

Step 2
When the system pressure reaches 600 bar, the pump continues to pump at a
flow rate that keeps the pressure constant. The flow that is needed to keep the
pressure constant is directly translated into a leak rate.

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7 Test Functions and Calibration
(System) Pressure Test

Positioning the Blank Nut

To test the complete system’s pressure tightness, the blank nut should be
positioned at the column compartment outlet (or the outlet of the last module
before the detector).
If a specific component is suspected of causing a system leak, place the blank
nut immediately before the suspected component, and then run the System
Pressure Test again. If the test passes, the defective component is located after
the blank nut. Confirm the diagnosis by placing the blank nut immediately after
the suspected component. The diagnosis is confirmed if the test fails.

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7 Test Functions and Calibration
(System) Pressure Test

Running the System Pressure Test

Running the test from the Agilent Lab Advisor

When The test should be used when problems with small leaks are suspected, or after maintenance of flow
path components (e.g., pump seals, injection seal) to prove pressure tightness up to 600 bar

Parts required p/n Description

5043-0277
A Blank nut long 10-32, PEEK with stainless steel core

Preparations Place two bottles of HPLC-grade water in channels A and B (A1 and B1 if the pump is equipped with
a solvent selection valve)

Make absolutely sure that all parts of the flow path that are part of the test are
NOTE very thoroughly flushed with water before starting to pressurize the system! Any
trace of other solvents or the smallest air bubble inside the flow path definitely
will cause the test to fail!

1 Select the system pressure test from the test selection menu.
2 Start the test and follow the instructions.

Make sure to release the pressure by opening the purge valve when the test has
NOTE completed. Otherwise the pump may generate an overpressure error.

Evaluating the Results

The sum of all leaks between the pump and the blank nut will add up to the total
leak rate. Note that small leaks may cause the test to fail, but solvent may not be
seen leaking from a module.

Please notice the difference between an error in the test and a failure of the test!
NOTE An error is caused by the abnormal termination during the operation of the test
whereas a failure of a test indicates that the test results were not within the
specified limits.

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7 Test Functions and Calibration
(System) Pressure Test

If the pressure test fails:

• Ensure all fittings between the pump and the blank nut are tight. Repeat the
pressure test.

Often it is only a damaged blank nut itself (poorly shaped from overtightening)
NOTE that causes the test to fail. Before investigating on any other possible sources of
failure make sure that the blank nut you are using is in good condition and
properly tightened!

• If the test fails again, insert the blank nut at the outlet of the previous module
in the stack (e.g. autosampler, port 6 of the injection valve), and repeat the
pressure test. Exclude each module one by one to determine which module is
leaking.
• If the pump is determined to be the source of the leak, run the valve test to
identify the defective pump component.

Potential Causes of Pressure Test Failure

After isolating and fixing the cause of the leak, repeat the pressure test to confirm
the system is pressure tight.
Table 9 Potential Cause (Pump)

Potential Cause (Pump) Corrective Action

Purge valve open. Close the purge valve.

Loose or leaky fitting. Tighten the fitting or exchange the capillary.

Damaged pump seals or pistons. Run the valve test to identify the defective
component.

Loose purge valve. Tighten the purge valve nut (14 mm wrench).

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7 Test Functions and Calibration
(System) Pressure Test

Table 10 Potential Cause (Autosampler)

Potential Cause (Autosampler) Corrective Action

Loose or leaky fitting. Tighten or exchange the fitting or capillary.

Rotor seal (injection valve). Exchange the rotor seal.

Damaged metering seal or piston. Exchange the metering seal. Check the piston for
scratches. Exchange the piston if required.

Needle seat. Exchange the needle seat.

Table 11 Potential Cause (Column Compartment)

Potential Cause (Column Compartment) Corrective Action

Loose or leaky fitting. Tighten or exchange the fitting or capillary.

Rotor seal (column switching valve). Exchange the rotor seal.

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7 Test Functions and Calibration
Valve Test

Valve Test

Description
The Valve Test provides a fast and accurate way to verify proper hydraulic
operation of the binary pump. Problems that are related to defective valves, seals
or pistons can be diagnosed and usually the defective part is identified.

Step 1
The system is setup with water on both channels and a restriction capillary is
attached to the outlet of the pump. Pump head A is delivering at 1 mL/min. The
pressure signal is monitored and overlaid with the piston movement plot. The
pressure pattern and the slope of the pressure signal are evaluated for the
delivery strokes of both pistons.

Step 2
The procedure from step 1 is repeated on pump head B.

Step 3
The data from step 1 and 2 are evaluated. In case test failed, a conclusion about
the defective part is made.

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7 Test Functions and Calibration
Valve Test

Running the Valve Test

Running the test from the Agilent Lab Advisor

When The test should be used to prove proper operation of the binary pump after repairs or when the
pressure test (see “(System) Pressure Test” on page 93) determined a problem with the pump.

Tools required p/n Description

8710-0510
A Wrench, 1/4 - 5/16 inch, open

Parts required p/n Description

G1312-67500
A Calibration capillary assembly

Preparations Place two bottles of HPLC-grade water in channels A and B (A1 and B1 if the pump is equipped with
a solvent selection valve)

The Valve Test should only be performed when firmware revisions A.06.50 or
NOTE below are installed.
For firmware revisions above A.06.50, perform the Leak Rate Test instead.

Make absolutely sure that the pump is very thoroughly flushed with water before
NOTE starting the test! Any trace of other solvents or the smallest air bubble inside the
flow path definitely will cause the test to generate misleading results!

1 Select the valve test from the test selection menu.

2 Start the test and follow the instructions.

Make sure to release the pressure by opening the purge valve when the test has
NOTE completed. Otherwise the pump may generate an overpressure error.

Evaluating the Results

Refer to the help file of the Agilent Lab Advisor for further details.

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7 Test Functions and Calibration
Binary Pump Solvent Compressibility Calibration

Binary Pump Solvent Compressibility Calibration

Description
Each solvent or solvent mixture has unique compressibility at different
pressures. In order to deliver accurate flow with minimal pressure- and
composition ripple over the full operational pressure range, it is necessary that
the pump compensates precisely for the compressibility of the solvents in use.
The binary pump comes with compressibility parameters for the most common
HPLC solvents and solvent mixtures. If a solvent is not available in the list of
pre-calibrated solvents, the solvent compressibility calibration allows the
appropriate compressibility data to be generated.

Technical background
The solvent compressibility calibration relies on an accurate elasticity calibration
of the pump. With a proper elasticity calibration in place, the pump is switched
into pressure control mode. A restriction capillary is connected to the purge valve
outlet. By varying the flow rate, the pump maintains a certain pressure. The pump
optimizes the compressibility value of the solvent until the lowest possible pump
ripple is reached. The pump increases the flow rate and adjusts the pressure to
the next calibration step where the pump ripple is minimized again. This process
is repeated until solvent compressibility data for the whole operating pressure
range of the pump are available.
The compressibility data set for this solvent is stored in an XML-file on the
desktop. It can be shared with other InfinityLab LC Series binary pumps via the
controlling data system.

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7 Test Functions and Calibration
Binary Pump Solvent Compressibility Calibration

Running the Solvent Compressibility Calibration

Running the Solvent Compressibility Calibration from the Agilent Lab Advisor

When If a solvent is not available in the list of pre-calibrated solvents, the solvent compressibility calibration
allows to generate appropriate compressibility data.

Tools required p/n Description

8710-0510
A Wrench, 1/4 - 5/16 inch, open

Parts required p/n Description

G1312-67500
A Calibration capillary assembly

Preparations Place a bottles with solvent to be calibrated in channel A (resp. A1 if a solvent selection valve is
installed).

Avoid inaccurate pump elasticity calibration.

C AU T I O N
This would lead into invalid and not-portable solvent compressibility data.
 Make sure to perform accurate pump elasticity calibration.

Make absolutely sure that the pump is very thoroughly flushed with the solvent to
NOTE be calibrated before starting the procedure! Any trace of other solvents or the
smallest air bubble inside the flow path definitely will cause the calibration to fail!

1 Select the solvent from the test selection menu.

2 Start the test and follow the instructions.

Make sure to release the pressure by opening the purge valve when the test has
NOTE completed. Otherwise the pump may generate an overpressure error.

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7 Test Functions and Calibration
Pump Elasticity Calibration

Pump Elasticity Calibration

Description
The flow path components of the binary pump have an inherent and pressure
dependent elasticity which differs from pump to pump. When this
elasticity/pressure function is known, a correction algorithm can be applied. This
results in significantly improved pump performance in low delay volume mode
(damper and mixer bypassed).
The pump elasticity calibration uses a solvent with well known properties
(HPLC-grade water) to determine the pump elasticity over the entire operating
pressure range and stores the calibration values in the non-volatile RAM of the
pump mainboard.
The initial calibration is done at the factory. It only needs to be repeated after
replacement of major pump parts (mainboard, pump drive). The test allows to
define which pump head will be calibrated.

Results of the pump elasticity calibration rely on known compressibility

NOTE parameters for pure water. If the water is not HPLC-grade, not well degassed or
degasser and pump are not flushed properly, the pump elasticity calibration will
fail. The pump elasticity calibration has to be performed for each pump head
individually.

Incorrect pump elasticity calibration.

C AU T I O N
Solvent compressibility calibrations acquired with a miscalibrated pump will
work, but they are not transferable to other pumps. A correct pump elasticity
calibration is an essential prerequisite for successful solvent compressibility
calibrations.
 Calibrate the pump elasticity correctly.

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7 Test Functions and Calibration
Pump Elasticity Calibration

Running the Pump Elasticity Calibration

Running the Pump Elasticity Calibration from the Agilent Lab Advisor Software

When The initial calibration is done at the factory. It only needs to be repeated after replacement of major
pump parts (mainboard, pump drive).

Tools required p/n Description

8710-0510
A Wrench, 1/4 - 5/16 inch, open

Parts required p/n Description

G1312-67500
A Calibration capillary assembly

Preparations Place all bottle heads in to a bottle of HPLC-grade water.

Make absolutely sure that the pump is very thoroughly flushed with the solvent to
NOTE be calibrated before starting the procedure! Any trace of other solvents or the
smallest air bubble inside the flow path definitely will cause the calibration to fail!

If a solvent selection valve is installed flush all four solvent channels to avoid that
NOTE air from a dry solvent intake tube is drawn into the flow path upon initialization.

1 Select the pump elasticity calibration from the test selection menu.
2 Start the test and follow the instructions.

Make sure to release the pressure by opening the purge valve when the test has
NOTE completed. Otherwise the pump may generate an overpressure error.

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7 Test Functions and Calibration
Pump Leak Rate Test

Pump Leak Rate Test

Introduction
The Pump Leak Rate Test is used for verifying the internal tightness of the pump
and helps identifying parts which may have caused a leak.
Minimum firmware revisions:
• D.07.01

Running the Test

Parts required p/n Description

5043-0277
A Blank nut long 10-32, PEEK with stainless steel core

1 Select the Pump Leak Rate Test from the Test Selection menu.
2 Start the test and follow the instructions.

Make sure to release the pressure by slowly opening the purge valve when the
NOTE test has finished.

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7 Test Functions and Calibration
Pump Leak Rate Test

Evaluating the Results

Results of the leak rate test are the leak rates measured for pistons 1 and 2 as
described for the test principle. If any of the leak rates exceeds 3 µL/min, the test
will fail.

to column

Damper

Outlet valve

Purge valve

Inlet valve to waste

Seals

Pump chamber 1 Pump chamber 2

Piston 1

Piston 2

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7 Test Functions and Calibration
Pump Leak Rate Test

Potential Causes of Leak Rate Test Failure

For binary pumps, secondary pump chambers are connected. A leak observed in
NOTE any of these chambers may be caused by the other secondary pump chamber.

Secondary Leak
If a leak is found for movement of piston 2 (secondary leak), the following
reasons are possible:

Probable cause Suggested actions

1 System not flushed properly Flush system for several minutes

2 Degassing efficiency is low Check degasser performance

3 Purge valve not closed or defect Check purge valve

4 Blank nut not installed tightly Tighten or replace blank nut

5 Outlet valve leaking (read below) Replace outlet valve

6 Leak at piston 2 or seal in chamber 2 Inspect piston, replace piston and/or seal

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7 Test Functions and Calibration
Pump Leak Rate Test

Primary Leak
If a leak is found for movement of piston 1 (primary leak), any leak described for
piston movement 2 will cause a failure for piston 1 as well, as the liquid can move
through the outlet valve to chamber 2. Such cases need to be identified as
described before. Additionally, following causes are possible:

Probable cause Suggested actions

1 Leak at piston 1 or seal in chamber 1 Inspect piston, replace piston and/or seal

2 Leak at inlet valve Replace inlet valve or inlet valve cartridge (AIV
only)

Internal Outlet Valve Leak

A leak of the outlet valve will be identified separately (internal outlet valve leak) by
calculating the difference between leak rate 1 and leak rate 2. If the second leak
rate is higher than the first one, this is due to a flow back through the outlet valve.

Probable cause Suggested actions

1 Leak at outlet valve Replace the part which has failed and re-run the
test.

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8 Maintenance

Introduction to Maintenance 109

Cautions and Warnings 110
Cleaning the Module 112
Overview of Maintenance and Simple Repairs 113
Maintenance Procedures 114
Remove and Install Doors 115
Exchange the Purge Valve Frit or the Purge Valve 117
Replace the O-Ring on the Purge Valve 120
Remove the Pump Head Assembly 122
Maintenance of a Pump Head without Seal Wash 124
Maintenance of a Pump Head with Seal Wash 127
Reinstall the Pump Head Assembly 131
Seal Wear-in Procedure 133
Exchange the Active Inlet Valve (AIV) or its Cartridge 134
Exchange the Seal Wash Cartridge 137
Replace Leak Handling System Parts 139
Exchange the Outlet Valve 141
Installation of the Solvent Selection Valve Upgrade Kit 143
Exchange the Solvent Selection Valve 145
Replacing Module Firmware 148

This chapter describes the maintenance of the module.

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8 Maintenance
Introduction to Maintenance

Introduction to Maintenance

The pump is designed for easy maintenance. The most frequent maintenance
procedures such as piston seal replacement and purge valve frit exchange can
be done from the front side without removing the pump from the system stack.
These procedures are described in “Overview of Maintenance and Simple
Repairs” on page 113.

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8 Maintenance
Cautions and Warnings

Cautions and Warnings

The module is partially energized when switched off, as long as the power
WAR N IN G
cord is plugged in.
Repair work at the module can lead to personal injuries, e.g. electrical
shock, when the cover is opened and the module is connected to power.
 Always unplug the power cable before opening the cover.
 Do not connect the power cable to the instrument while the covers are
removed.

Sharp metal edges

WAR N IN G
Sharp-edged parts of the equipment may cause injuries.
 To prevent personal injury, be careful when getting in contact with sharp
metal areas.

Toxic, flammable and hazardous solvents, samples and reagents

WAR N IN G
The handling of solvents, samples and reagents can hold health and safety
risks.
 When working with these substances observe appropriate safety
procedures (for example by wearing goggles, safety gloves and
protective clothing) as described in the material handling and safety
data sheet supplied by the vendor, and follow good laboratory practice.
 The volume of substances should be reduced to the minimum required
for the analysis.
 Do not operate the instrument in an explosive atmosphere.

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8 Maintenance
Cautions and Warnings

Electronic boards and components are sensitive to electrostatic discharge

C AU T I O N (ESD).
ESD can damage electronic boards and components.
 Be sure to hold the board by the edges, and do not touch the electrical
components. Always use ESD protection (for example, an ESD wrist strap)
when handling electronic boards and components.

Safety standards for external equipment

C AU T I O N
 If you connect external equipment to the instrument, make sure that you
only use accessory units tested and approved according to the safety
standards appropriate for the type of external equipment.

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8 Maintenance
Cleaning the Module

Cleaning the Module

To keep the module case clean, use a soft cloth slightly dampened with water, or
a solution of water and mild detergent. Avoid using organic solvents for cleaning
purposes. They can cause damage to plastic parts.

Liquid dripping into the electronic compartment of your module can cause
WAR N IN G
shock hazard and damage the module
 Do not use an excessively damp cloth during cleaning.
 Drain all solvent lines before opening any connections in the flow path.

A solution of 70 % isopropanol and 30 % water might be used if the surface of the

NOTE module needs to be disinfected.

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8 Maintenance
Overview of Maintenance and Simple Repairs

Overview of Maintenance and Simple Repairs

Figure 10 on page 113 shows the main user accessible assemblies of the binary
pump. The pump heads and its parts require normal maintenance (for example,
seal exchange) and can be accessed from the front (simple repairs).
Replacement of valve cartridges or filters don’t require to remove the pump from
the system stack.

Active seal wash cartridge

Solvent selection valve

Pressure sensor
Purge valve
Outlet valve

Active inlet valve

Pump head

Figure 10 Overview of Maintenance and Simple Repairs

1 Purge valve, see “Exchange the Purge Valve Frit or the Purge Valve” on page 117

2 Outlet valve, see “Exchange the Outlet Valve” on page 141

3 Active inlet valve, see “Exchange the Active Inlet Valve (AIV) or its Cartridge” on page 134

4 Pump head, see “Maintenance of a Pump Head with Seal Wash” on page 127

5 Solvent selection valve, see “Exchange the Solvent Selection Valve” on page 145

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8 Maintenance
Maintenance Procedures

Maintenance Procedures

The procedures described in this section can be done with the binary pump in
place in the system stack.
Table 12 Maintenance procedures

Procedure Typical Frequency Notes

“Exchange the Purge Valve Frit or the Purge Yearly, or if the frit shows A pressure drop of > 10 bar in low delay volume
Valve” on page 117 indication of configuration and > 20 bar in standard
contamination or configuration across the frit (5 mL/min H 2O with
blockage purge valve open) indicates blockage
If internally leaking Solvent dripping out of waste outlet when valve is
closed

“Remove the Pump Head Assembly” on During yearly Necessary to get access to pump seals and
page 122 maintenance pistons

“Maintenance of a Pump Head without Seal Yearly, or if pump Leaks at lower pump head side, unstable
Wash” on page 124 performance indicates retention times, pressure ripple unstable — run
seal wear Pump Leak Rate Test for verification
Seal life time shorter than normally expected —
check pistons while changing the seals

“Maintenance of a Pump Head with Seal Yearly, or if pump Only necessary when Seal Wash Option is
Wash” on page 127 performance indicates installed. Leaks at lower pump head side, loss of
seal wear wash solvent

“Exchange the Active Inlet Valve (AIV) or its If leaking externally Error messages “Inlet Valve Fuse” or “Inlet Valve
Cartridge” on page 134 If solenoid is defective Missing”

“Exchange the Outlet Valve” on page 141 If internally leaking Pressure ripple unstable, run Pump Leak Rate
Test for verification

“Exchange the Solvent Selection Valve” on If internally leaking Cross port flow
page 145 If solenoid is defective Error message “Valve Failed”

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8 Maintenance
Remove and Install Doors

Remove and Install Doors

Parts required p/n Description

5067-5746A Door Assembly Infinity 180 Right

5067-5745A Door Assembly Infinity 180 Left

The figures shown in this procedure exemplarily show the Infinity II Multisampler
NOTE module.
The principle of how to remove and/or install doors works in the same way for all
Infinity II modules.

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8 Maintenance
Remove and Install Doors

1 Press the release buttons and pull the front door out. 2 For the Installation of the front door. Insert the hinges
into their guides and move the door in until the release
buttons click into their final position.

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8 Maintenance
Exchange the Purge Valve Frit or the Purge Valve

Exchange the Purge Valve Frit or the Purge Valve

When • Frit – when piston seals are exchanged or when contaminated or blocked (pressure drop of
> 10 bar in low delay volume configuration and > 20 bar in standard configuration across the frit
at a flow rate of 5 mL/min of water with purge valve opened)
• Purge valve – if internally leaking

Tools required p/n Description

8710-0510A Open-end wrench 1/4 — 5/16 inch

8710-1924A Open-end wrench 14 mm

Pair of tweezers
OR Toothpick

Parts required p/n Description

01018-22707
A PTFE frits (pack of 5)

G7111-60061
A Purge valve

5067-4728
A Seal cap

Preparations • Switch off pump at the main power switch

• Open the doors
• Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding
leakages

1 Using a 1/4 inch wrench disconnect the pump outlet 2 Using the 14 mm wrench, unscrew the purge valve and
capillary from the purge valve. Disconnect the waste remove it from the purge valve holder.
tube. Beware of leaking solvents due to hydrostatic
pressure.

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8 Maintenance
Exchange the Purge Valve Frit or the Purge Valve

3 Remove the seal cap from the purge valve. 4 Using a pair of tweezers or a toothpick remove the frit.

Seal cap

Frit

5 Place a new frit into the purge valve with the orientation 6 Reinstall the seal cap including the gold seal.
of the frit as shown below (slit in frit points to the front).

Seal cap NOTE

Before reinstallation always check the gold seal in the seal
Frit cap. A deformed seal cap should be exchanged.

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8 Maintenance
Exchange the Purge Valve Frit or the Purge Valve

7 Insert the purge valve into the purge valve holder.

C AU T I O N
Damage to the purge valve

 Do not lift the pump using the purge valve as a handle,

it might get leaky.

 Do not try to turn the purge valve into the correct

position when already fixed to the pump. The rubber
o-ring might break.

 Anticipate the correct position of the connections

before tightening the valve.
8 Tighten the purge valve and reconnect outlet capillary
and waste tubing.

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8 Maintenance
Replace the O-Ring on the Purge Valve

Replace the O-Ring on the Purge Valve

When If the original o-ring is damaged and needs to be replaced

Tools required p/n Description

8710-0510
A Open-end wrench 1/4 — 5/16 inch

8710-1924
A Open-end wrench 14 mm

Pair of tweezers
OR Toothpick

Parts required # p/n Description

1 5067-6595A 1260 PV O-ring FKM 5/pack

1 01018-22707A PTFE frits (pack of 5) (OPTIONAL)

1 5067-4728A Seal cap (OPTIONAL)

Preparations • Switch off pump at the main power switch.

• Open the doors of the module.
• Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding
leakages.
• Remove the purge valve from the pump head.

1 Disassemble the purge valve. 2 Remove the old o-ring from the purge valve.
3 Clean the purge valve parts.

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8 Maintenance
Replace the O-Ring on the Purge Valve

4 Place the new o-ring on the Screw Purge Valve. 5 Place the screw with o-ring on the Purge Valve Body.

6 Place the mounting ring on the screw and push down 7 Push the screw up and guide the o-ring into the gap.
the o-ring.

8 Place a new frit into the purge valve with the orientation 9 Install the purge valve to the pump. Make sure not to
of the frit as shown below (slit in frit points to the front). turn the purge valve body when the screw is fixed to the
Reinstall the seal cap including the gold seal. pump. The o-ring will take damage.

NOTE
Before reinstallation always check the gold seal in the seal
cap. A deformed seal cap should be exchanged.

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8 Maintenance
Remove the Pump Head Assembly

Remove the Pump Head Assembly

When • Exchanging pump seals

• Exchanging pistons
• Exchanging seals of the seal wash option

Tools required p/n Description

8710-0510
A Open-end wrench 1/4 — 5/16 inch

Hexagonal key, 4 mm
5023-0240
A Hex driver, ¼", slitted

Preparations Switch off the pump at the main power switch.

Damage of the pump drive

C AU T I O N
Starting the pump when the pump head is removed may damage the pump
drive.
 Never start the pump when the pump head is removed.

Both pump head assemblies use the same internal components. In addition,
NOTE pump head A is fitted with the purge valve. The following procedure describes
the removal and disassembly of pump head A (left). For pump head B (right)
proceed in the same way and skip steps that deal with the purge valve.

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8 Maintenance
Remove the Pump Head Assembly

1 Open the doors. 2 Disconnect the capillaries at the back of the purge valve
holder, the pump head adapter and the tube at the
active inlet valve. Beware of leaking solvents.

3 Using a 4 mm hexagonal key stepwise loosen and 4 Unplug the active inlet valve cable from the connector.
remove the two pump head screws. Remove the pump head assembly from the module.

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8 Maintenance
Maintenance of a Pump Head without Seal Wash

Maintenance of a Pump Head without Seal

Wash

When In case of maintenance or pump head internal leaks

Tools required p/n Description

8710-0510 A Open-end wrench 1/4 — 5/16 inch

Hexagonal key, 4 mm
01018-23702
A Insert tool

Parts required # p/n Description

1 5063-6589
A Piston seal PTFE, carbon filled, black (pack of 2), default

OR 1 0905-1420
A PE seals (pack of 2)

1 5063-6586
A Sapphire piston

Preparations • Switch off the pump at the main power switch.

• Open the doors of the module.
• Use an optional solvent shutoff valve or lift up solvent filters for avoiding leakages.
• Remove the Pump Head Assembly.

Both pump head assemblies use the same internal components. In addition,
NOTE pump head A is fitted with the purge valve. The following procedure describes
the removal and disassembly of pump head A (left). For pump head B (right)
proceed in the same way and skip steps that deal with the purge valve.

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8 Maintenance
Maintenance of a Pump Head without Seal Wash

1 Place the pump head on a flat surface. Loosen the lock 2 While holding the lower half of the assembly (piston
screw (two turns). housing), carefully pull the pump chamber housing
away from the piston housing.

3 Remove the support rings from the piston housing. 4 Lift the housing away from the pistons.

5 Check the piston surface and remove any deposits or 6 Using the steel side of the insert tool, carefully remove
layers: clean the piston surface with abrasive paper and the seal from the pump housing.
rinse with 2-propanol. Replace piston if scratched.

Piston Surface

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8 Maintenance
Maintenance of a Pump Head without Seal Wash

7 Using the plastic side of the insert tool, insert new seals 8 Place the support rings on the piston housing. Note the
into the pump head. correct position of the pins.

9 Place the pump housing onto the piston housing. 10 Insert the pistons and carefully press them into the
seals.

11 Tighten the lock screw. 12 Install the pump head, see “Reinstall the Pump Head
Assembly” on page 131.

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8 Maintenance
Maintenance of a Pump Head with Seal Wash

Maintenance of a Pump Head with Seal Wash

When In case of maintenance or pump head internal leaks

Tools required p/n Description

Hexagonal key, 4 mm
01018-23702
A Insert tool

Screwdriver, small flat head

Parts required # p/n Description

1 5063-6589A Piston seal PTFE, carbon filled, black (pack of 2), default

OR 1 0905-1420A PE seals (pack of 2)

1 5062-2484A Gasket, seal wash (pack of 6)

1 0905-1175A Wash seal (PTFE)

OR 1 0905-1718A Wash Seal PE

1 5063-6586A Sapphire piston

Preparations • Switch off the pump at the main power switch.

• Open the doors of the module.
• Use an optional solvent shutoff valve or lift up solvent filters for avoiding leakages.
• Remove the Pump Head Assembly.
• Remove the wash solvent tubings from the support ring inlet and outlet.

Both pump head assemblies use the same internal components. In addition,
NOTE pump head A is fitted with the purge valve. The following procedure describes
the removal and disassembly of pump head A (left). For pump head B (right)
proceed in the same way and skip steps that deal with the purge valve.

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8 Maintenance
Maintenance of a Pump Head with Seal Wash

1 Place the pump head on a flat surface. Loosen the lock 2 While holding the lower half of the assembly (piston
screw (two turns). housing), carefully pull the pump housing away from the
piston housing.

3 Remove the seal holder and the seal wash support rings 4 Remove the seal holder from the support ring
from the piston housing. assembly.

5 Lift the housing away from the pistons. 6 Check the piston surface and remove any deposits or
layers: clean the piston surface with abrasive paper and
rinse with 2-propanol. Replace piston if scratched.

Piston Surface

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8 Maintenance
Maintenance of a Pump Head with Seal Wash

7 Using the steel side of the insert tool, carefully remove 8 Using the plastic side of the insert tool, insert new seals
the seal from the pump housing. into the pump head.

9 Using the steel side of the insert tool, remove the seal 10 Using the plastic side of the insert tool, press the new
wash gasket and the wash seal from the support ring. wash seal (spring pointing upwards) into the recess of
the support ring.

11 Place a seal wash gasket in the recess of the support 12 Place the support rings on the piston housing. Note the
ring. Use a matching orientation of gasket and support correct position of the pins on the support ring.
ring. Put the seal holder on top of the gasket.

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8 Maintenance
Maintenance of a Pump Head with Seal Wash

13 Place the pump chamber housing onto the piston 14 Insert the pistons and carefully press them into the
housing. seals.

15 Tighten the lock screw. 16 Install the pump head, see “Reinstall the Pump Head
Assembly” on page 131.

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8 Maintenance
Reinstall the Pump Head Assembly

Reinstall the Pump Head Assembly

When When reassembling the pump

Tools required p/n Description

8710-0510
A Open-end wrench 1/4 — 5/16 inch

Hexagonal key, 4 mm
5023-0240
A Hex driver, ¼", slitted

Parts required # p/n Description

1 79846-65501
A Pump head grease

Damage of the pump drive

C AU T I O N
Starting the pump when the pump head is removed may damage the pump
drive.
 Never start the pump when the pump head is removed.

1 Slide the pump head assembly onto the pump drive. 2 Using a 4 mm hexagonal key, tighten the pump head
Reconnect the active inlet valve cable to the connector. screws stepwise with increasing torque.

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8 Maintenance
Reinstall the Pump Head Assembly

3 Reconnect all tubings and capillaries. 4 Close the doors.

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8 Maintenance
Seal Wear-in Procedure

Seal Wear-in Procedure

1 Put a bottle with 100 ml of isopropanol in the solvent cabinet and place the
solvent intake filter of the pump head you want to wear in into this bottle.
2 Screw the PEEK adapter 1/4-28 to 10-32 (0100-1847) onto the active inlet
valve and connect the inlet tube from the bottle head directly to it.
3 Connect the Restriction capillary (5022-2159) to the purge valve. Connect its
other end to a waste container.
4 Open the purge valve and purge the system for 5 min with isopropanol at a
flow rate of 2 mL/min.
5 Close the purge valve, set the flow to a value that gives a pressure of 580 bar.
Pump 15 min at this pressure to wear the seals in. The pressure can be
monitored with the Instant Pilot, chromatographic data system or any other
controlling device connected to your pump.
6 Turn OFF the pump, slowly open the purge valve to release the pressure from
the system, disconnect the restriction capillary and reconnect the outlet
capillary to the purge valve. Reconnect the intake tubing to the solvent
selection valve and the connecting tube from the solvent selection valve
(if installed) to the AIV.
7 Purge your system with the solvent used for your next application.

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8 Maintenance
Exchange the Active Inlet Valve (AIV) or its Cartridge

Exchange the Active Inlet Valve (AIV) or its

Cartridge

When If internally leaking (backflow)

Tools required p/n Description

8710-1924 A Open-end wrench 14 mm

Parts required p/n Description

G1312-60025
A Active inlet valve body, without cartridge

G1312-60020A Cartridge for active inlet valve 600 bar

Preparations Switch off the pump at the main power switch

Ensure correct fit of the active inlet valve

C AU T I O N
Overtightening will destroy the active inlet valve cartridge.
 Tighten the active inlet valve properly.

1 Open the doors. 2 Unplug the active inlet valve cable from the connector.

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8 Maintenance
Exchange the Active Inlet Valve (AIV) or its Cartridge

3 Disconnect the solvent inlet tube at the active inlet valve 4 Using a 14 mm wrench, loosen the active inlet valve and
(beware of leaking solvents). remove the valve from the pump head.

5 Using a pair of tweezers, remove the valve cartridge 6 Push the cartridge into the new active inlet valve.
from the defective active inlet valve.

7 Screw the new valve into the pump head. With the 8 Position the valve so that the solvent inlet tube
14 mm wrench, turn the nut until it is hand tight. connection points towards the front.

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8 Maintenance
Exchange the Active Inlet Valve (AIV) or its Cartridge

9 Using the 14 mm wrench, tighten the nut by turning the 10 Reconnect the inlet tube to the valve.
valve in its final position (not more than a quarter turn).
Do not overtighten the valve.

11 Reconnect the Active Inlet Valve cable to the connector 12 Close the doors.
in the Z-panel.

After an exchange of the valve it may be required to pump several mL of the

NOTE solvent used in the current application before the flow stabilizes at a pressure
ripple as low as it used to be when the system was still working properly.

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8 Maintenance
Exchange the Seal Wash Cartridge

Exchange the Seal Wash Cartridge

Parts required p/n Description

5065-4445 A Peristaltic pump with PharMed tubing

Preparations • Switch off pump at the main power switch.

• Open the doors.

1 Remove the wash solvent tubings from the support ring 2 Unclip the peristaltic pump cartridge from the module
outlet and from the adapter leading to the waste bottle. housing and remove it.

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8 Maintenance
Exchange the Seal Wash Cartridge

3 Put the new peristaltic pump cartridge onto the rod of 4 Connect the peristaltic pump tubes to the support rings
the pump motor and push the plastic clips into the outlet and to the adapter leading to the waste bottle.
module housing.

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8 Maintenance
Replace Leak Handling System Parts

Replace Leak Handling System Parts

Parts required p/n Description

5063-6527 A Tubing, Silicon Rubber, 1.2 m, ID/OD 6/9 mm
approximately 85 mm required

1 Open the doors. 2 Press the Leak Adapter down (1.) and remove it
together with the tubing (2.).

1.
2.

3 Install the Leak Adapter by pressing it into the Main 4 Insert the Tubing (approximately 85 mm required for
Cover. replacement) between Leak Adapter outlet and Leak
Panel.

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8 Maintenance
Replace Leak Handling System Parts

5 Close the doors.

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8 Maintenance
Exchange the Outlet Valve

Exchange the Outlet Valve

When if leaking internally

Tools required p/n Description

8710-1924
A Open-end wrench 14 mm

8710-0510
A Open-end wrench 1/4 — 5/16 inch

5067-5688
A Torque wrench 1 – 25 Nm with 14 mm wrench

Parts required # p/n Description

1 G1312-60067
A Outlet valve (standard)

OR 1 G1312-60167
A Outlet valve (type N/SFC)

Preparations Switch off the pump at the main power switch

1 Using a ¼ inch wrench disconnect the absorber 2 Unscrew the valve with the 14 mm wrench and remove
capillary from the outlet valve. it from the pump body.

NOTE
Do not disassemble the outlet valve, as this can damage the
valve.

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8 Maintenance
Exchange the Outlet Valve

3 Reinstall the outlet valve and tighten it using a torque 4 Reconnect the capillary.
wrench (approx.12 Nm).

approx.
12 Nm

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8 Maintenance
Installation of the Solvent Selection Valve Upgrade Kit

Installation of the Solvent Selection Valve

Upgrade Kit

A solvent selection valve allows you to choose between four different solvents
that can be used with a binary pump. The valve switches between two solvents
A1 and A2 for channel A of the left pump head and two solvents B1 and B2 for
channel B of the right pump head.

Tools required p/n Description

8710-0899
A Screwdriver Pozidrive Shaft

Parts required # p/n Description

1 G1381-60001
A Solvent Selection Valve Upgrade Kit
includes:
1 Valve Assembly (1 Valve Holder, 1 Cable, 1 Cable Holder, 4 Screws,
4 Plugs)
2 Bottles
2 Bottle Heads
2 Tubings
1 Distance sheet SSV

Preparations Remove the solvent tubes from the Degasser

1 Break out the metal sheet piece to get access to the 2 Guide the SSV cable through the slots in the distance
connection socket inside (behind the pump front metal sheet and route it through the slot in the pump front
panel). metal panel to the internal connection socket.

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8 Maintenance
Installation of the Solvent Selection Valve Upgrade Kit

3 Carefully plug in the connector of the solvent selection 4 Install the solvent selection assembly by fixing the
valve into the internal connection socket on the left side indicated screws with distance sheet under the SSV
of the slot behind the pump front metal panel. body.

5 Connect the outlet of solvent A (upper row) to the top

degasser inlet and the outlet of solvent B (lower row) to
the bottom degasser inlet. Put the solvent bottles into
the solvent cabinet. Connect the bottle heads of
solvents A1 and A2 to the inlets in the upper row, see
labels on valve assembly. Connect the bottle heads of
solvents B1 and B2 to the inlets in the lower row, see
labels on valve assembly.

Block unused channels of the SSV using a plug (Blank plug (5041-8365)) to avoid
NOTE leaks or air entering the solvent channels.

Before using of the system with a new installed valve it may be required to pump
NOTE several mL of solvent to get the flow stabilized at a pressure ripple as low as it
used to be when the system was still working properly.

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8 Maintenance
Exchange the Solvent Selection Valve

Exchange the Solvent Selection Valve

When If leaking internally (crossflow between the ports), or if one of the channels is blocked

Tools required p/n Description

8710-0899
A Screwdriver Pozidrive Shaft

Parts required p/n Description

5067-5895
A Solvent selection valve

Preparations Switch off the pump at the main power switch

1 Lift solvent bottles out of the solvent cabinet and place 2 Disconnect all tubings from the solvent selection valve.
them on the table. Disconnect the solvent tubes from
the solvent selection valve and empty the tubes into the
bottles. Place the bottles back into the solvent cabinet.

3 Using a screwdriver, loosen the holding screws of the 4 Carefully pull the valve holder out.
valve holder.

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8 Maintenance
Exchange the Solvent Selection Valve

5 Disconnect the valve cable from the internal connection 6 Completely remove the old valve.
socket by pressing the fixing clip through the left side
middle hole with the screwdriver.

7 Bend the cable at the connector of the new valve. 8 Guide the cable and connector into the hole and push
the connector into the socket.

9 Exchange the defective solvent selection valve. 10 Tighten the screws of the valve holder.

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8 Maintenance
Exchange the Solvent Selection Valve

11 Reconnect all tubings to the solvent selection valve.

After an exchange of the valve it may be required to pump several mL of solvent

NOTE before the flow stabilizes at a pressure ripple as low as it used to be when the
system was still working properly.

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8 Maintenance
Replacing Module Firmware

Replacing Module Firmware

When The installation of newer firmware might be necessary

• if a newer version solves problems of older versions or
• to keep all systems on the same (validated) revision.
The installation of older firmware might be necessary
• to keep all systems on the same (validated) revision or
• if a new module with newer firmware is added to a system or
• if third party control software requires a special version.

Tools required Description

Agilent Lab Advisor software

Parts required # Description

1 Firmware, tools and documentation from Agilent web site

Preparations Read update documentation provided with the Firmware Update Tool.

To upgrade/downgrade the module’s firmware carry out the following steps:

1 Download the required module firmware, the latest FW Update Tool and the
documentation from the Agilent web.
http://www.agilent.com/en-us/firmwareDownload?whid=69761
2 For loading the firmware into the module follow the instructions in the
documentation.
Module Specific Information
There is no specific information for this module.

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9 Parts and Materials for Maintenance

Hydraulic Path with Solvent Selection Valve 150

Hydraulic Path without Solvent Selection Valve 152
Pump Head Assembly Without Seal Wash 154
Pump Head Assembly with Seal Wash Option 156
Outlet Valve 158
Purge Valve Assembly 159
Active Inlet Valve Assembly 160
HPLC System Tool Kit 161
Active Seal Wash Option 162
Solvent Cabinet 163
Bottle Head Assembly 164
Accessory Kit 165
Cover Parts 166

This chapter lists all parts and tools that are required for maintenance and simple
repairs.

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9 Parts and Materials for Maintenance
Hydraulic Path with Solvent Selection Valve

Hydraulic Path with Solvent Selection Valve

Item # p/n Description

1 1 5067-5895 Solvent selection valve

1 5041-8365 Blank plug

for unused SSV channels

2 2 G7111-60100 Solvent Tubes including labels

Solvent selection valve to degasser
3 1 G7112-60070 Degasser Unit 2 Channels

4 1 G1311-67304 Connecting tube

Degasser to Channel A

5 1 G7112-67300 Connecting Tube

Degasser to Channel B

6 1 G1312-60025 Active inlet valve body, without cartridge

7 1 G1312-60045 Pump head assembly with seal wash

8 1 G1312-60067 Outlet valve

(standard)

OR 1 G1312-60167 Outlet valve (type N/SFC)

9 1 G1312-87300 Absorber capillary

10 1 G1312-67302 Capillary, channel A and B pump head outlet to mixing

chamber (included)

11 1 G1312-87301 Restriction capillary (mixing capillary to pressure sensor)

13 1 G1312-87305 Capillary SSL, 0.17 x 150 mm (pressure sensor to damper)

15 1 G1312-87330 Mixer

16 1 G1312-87306 Capillary SSL, 0.17 x 105 mm (connections to solvent

mixer)

1 G1312-04100 Bracket for solvent mixer

17 1 G7111-60061 Purge valve

18 1 5500-1246 Capillary ST 0.17 mm x 500 mm SI/SI

1 5500-1217 Capillary, ST, 0.17 mm x 900 mm SI/SX

19 1 5064-5444 Peristaltic pump cartridge, silicone tubing

1 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m

for seal wash option

20 1 5062-2461 Waste tube, 5 m (reorder pack)

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9 Parts and Materials for Maintenance
Hydraulic Path with Solvent Selection Valve

12 11 13 15 16

18

1
19

8 14
7 20

5 6 17 10 9
Figure 11 Hydraulic Path with Solvent Selection Valve

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9 Parts and Materials for Maintenance
Hydraulic Path without Solvent Selection Valve

Hydraulic Path without Solvent Selection Valve

Item p/n Description

1 G7112-60070 Degasser Unit 2 Channels

2 G1311-67304 Connecting tube

Degasser to Channel A

3 G7112-67300 Connecting Tube

Degasser to Channel B

4 G1312-60025 Active inlet valve body, without cartridge

5 G1312-60056 Pump Head 1200 SL without Seal Wash

6 G1312-60067 Outlet valve

(standard)

OR G1312-60167 Outlet valve (type N/SFC)

7 G1312-87300 Absorber capillary

8 G1312-67302 Capillary, channel A and B pump head outlet to mixing chamber

(included)

9 G1312-87301 Restriction capillary (mixing capillary to pressure sensor)

11 G1312-87305 Capillary SSL, 0.17 x 150 mm (pressure sensor to damper)

13 G1312-87330 Mixer

14 G1312-87306 Capillary SSL, 0.17 x 105 mm (connections to solvent mixer)

G1312-04100 Bracket for solvent mixer

15 G7111-60061 Purge valve

16 5500-1246 Capillary ST 0.17 mm x 500 mm SI/SI

5500-1217 Capillary, ST, 0.17 mm x 900 mm SI/SX

17 5065-4445 Peristaltic pump with PharMed tubing

18 5062-2461 Waste tube, 5 m (reorder pack)

5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m

for seal wash option

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9 Parts and Materials for Maintenance
Hydraulic Path without Solvent Selection Valve

10 9 11 13 14

17
16
12
6
18
5

4 15 8 7
Figure 12 Hydraulic Path without Solvent Selection Valve, with Active Seal Wash

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 153

9 Parts and Materials for Maintenance
Pump Head Assembly Without Seal Wash

Pump Head Assembly Without Seal Wash

Item p/n Description

G1312-60056 Pump Head 1200 SL without Seal Wash

1 5063-6586 Sapphire piston

2 G1311-60002 Piston housing

3a 5067-1560 Support Ring SL, no seal wash

(shown as one piece with 3b and 3c)

3b 5062-2484 Gasket, seal wash (pack of 6)

3c 5042-8952 Seal holder

Ceramic

4 G1312-87300 Absorber capillary

5 5063-6589 Piston seal PTFE, carbon filled, black (pack of 2), default

6 G1311-25200 Pump chamber housing

7 0515-0175 Mounting screw for manual purge valve holder, M4, 20 mm long

8 G1312-23200 Holder for manual purge valve

9 G7111-60061 Purge valve

10 G1312-60067 Outlet valve

(standard)

OR G1312-60167 Outlet valve (type N/SFC)

11 5042-1303 Lock screw

12 a G1312-60025 Active inlet valve body, without cartridge

12 b G1312-60020 Cartridge for active inlet valve 600 bar

13 G1312-23201 Adapter

14 0515-2118 Pump head screw (M5, 60 mm)

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9 Parts and Materials for Maintenance
Pump Head Assembly Without Seal Wash

7
8 1

9
2
10 3a
3b
11 3c

4
5
14

13 6

12 a,b

Figure 13 Pump Head Assembly Without Seal Wash

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 155

9 Parts and Materials for Maintenance
Pump Head Assembly with Seal Wash Option

Pump Head Assembly with Seal Wash Option

Item p/n Description

G1312-60045 Pump head assembly with seal wash

1 5065-9953 Seal wash pump assembly

5067-5744 Cabinet Kit 180 Infinity II

(includes sides, bottom, top, leak adapter top and Status
Indicator Insert)

5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m

for seal wash option

2 5063-6586 Sapphire piston

3 G1311-60002 Piston housing

4 01018-60027 Support ring seal wash

5 0905-1175 Wash seal (PTFE)

OR 0905-1718 Wash Seal PE

6 5062-2484 Gasket, seal wash (pack of 6)

7 5042-8952 Seal holder

8 G1312-87300 Absorber capillary

9 5063-6589 Piston seal PTFE, carbon filled, black (pack of 2), default
OR 0905-1420 PE seals (pack of 2)

10 0515-0175 Mounting screw for manual purge valve holder, M4, 20 mm long

11 G1312-23200 Holder for manual purge valve

12 G7111-60061 Purge valve

13 G1312-60067 Outlet valve

(standard)

OR G1312-60167 Outlet valve (type N/SFC)

14 5042-1303 Lock screw

15 G1311-25200 Pump chamber housing

16a G1312-60025 Active inlet valve body, without cartridge

16b G1312-60020 Cartridge for active inlet valve 600 bar

17 G1312-23201 Adapter

18 0515-2118 Pump head screw (M5, 60 mm)

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9 Parts and Materials for Maintenance
Pump Head Assembly with Seal Wash Option

10 2
11

12 3

5 4
13 6
14 7
8
9
18
17
15

16 a,b

Figure 14 Pump Head Assembly with Seal Wash Option

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 157

9 Parts and Materials for Maintenance
Outlet Valve

Outlet Valve

Item p/n Description

1 G1312-60067 Outlet valve
(standard)

OR 2 G1312-60167 Outlet valve (type N/SFC)

Figure 15 Outlet valve

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 158

9 Parts and Materials for Maintenance
Purge Valve Assembly

Purge Valve Assembly

Item p/n Description

1 G7111-60061 Purge valve

2 01018-22707 PTFE frits (pack of 5)

3 5067-4728 Seal cap

5067-6595 1260 PV O-ring FKM 5/pack

Figure 16 Purge Valve Assembly

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 159

9 Parts and Materials for Maintenance
Active Inlet Valve Assembly

Active Inlet Valve Assembly

Item p/n Description

1 G1312-60025 Active inlet valve body, without cartridge

2 G1312-60020 Cartridge for active inlet valve 600 bar

Figure 17 Active Inlet Valve Assembly

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 160

9 Parts and Materials for Maintenance
HPLC System Tool Kit

HPLC System Tool Kit

5023-2504
(Hex Driver SW-4 slitted)
5023-3138 5023-2503
(Reversible Screwdriver) (Hex Driver
SW-5 slitted)
5023-2502
(Hex Driver SW-6,
35/ ¼” slitted)

5023-3088
8710-1924
8710-2409
8710-0510
8710-0510
5023-2500
8710-1534

8710-2394
(Hex Key 9/64“, 15cm)

Box with:
9301-0411 (Syringe, Plastic) 5023-3089
9301-1337 (Syringe Adapter) Torx Key Set (T8,T9,T10,T15,T20,T25)
0100-1710 (Mounting tool for flangeless nut)
0100-1681 (Adapter luer/barb) 5023-2499
01018-23702 (Seal Insert tool) (Hex Key Set)
5067-6127 (Blank Nut V) 5043-1361
5023-2653 (Hex Key 3/32“) (Hex Key Set Driver)

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 161

9 Parts and Materials for Maintenance
Active Seal Wash Option

Active Seal Wash Option

The Active Seal Wash Option kit (G1399B) contains the following parts:

# p/n Description
1 5062-2484 Gasket, seal wash (pack of 6)

1 01018-23702 Insert tool

4 01018-60027 Support ring seal wash

2 0515-1508 Screws for Seal Wash Pump Motor

1 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m

4 0905-1175 Wash seal (PTFE)

1 5063-6589 Piston seal PTFE, carbon filled, black (pack of 2), default

1 1460-2763 Compression Spring ST

2 1520-0260 Shock mount

1 1540-0455 Edge protector

1 5041-2120 Folding box

1 5065-4445 Peristaltic pump with PharMed tubing

1 5042-6422 Tubing Connector, PP

1 5065-9943 Stepper Motor for the Peristaltic Pump

1 G3010-01203 RFI Strip 10×30

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 162

9 Parts and Materials for Maintenance
Solvent Cabinet

Solvent Cabinet

p/n Description
5067-6871 Solvent Cabinet Kit

For details refer to: Usage Guideline for the Solvent Cabinet (01200-90150)

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 163

9 Parts and Materials for Maintenance
Bottle Head Assembly

Bottle Head Assembly

The Bottle Head Assembly (G7120-60007) contains:

p/n Description
5063-6598 Ferrules with lock ring (10/Pk)

5063-6599 Tube screw (10/Pk)

Wire marker

5062-2483 Tube PTFE 1.5 mm x 5 m, 3 mm od

5062-8517 Inlet filter adapter (4/Pk)

5041-2168 Solvent inlet filter, 20 µm pore size

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 164

9 Parts and Materials for Maintenance
Accessory Kit

Accessory Kit

The Accessory Kit (G7111-68755) contains the following items:

Item # p/n Description

1 2 5043-1013 Tubing Clip

2 1 5181-1519 CAN cable, Agilent module to module, 1 m

3 1 5500-1246 Capillary ST 0.17 mm x 500 mm SI/SI

4 1 5500-1217 Capillary, ST, 0.17 mm x 900 mm SI/SX

5 3 5063-6527 Tubing, Silicon Rubber, 1.2 m, ID/OD 6/9 mm

6 1 G1311-90107 Algae note

7 3 5500-1169 Y Tube Connector ID 6.4

8 3 5500-1155 Tube Connector, 90 degree, ID 6.4

9 1 5043-1372 Tubing Connector Leak 3-1

10 2 5043-1373 Tubing Connector Leak Cap

11 2 0890-1195 Flexible sleeving 1.45 mm/2.5 mm, PTFE

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 165

9 Parts and Materials for Maintenance
Cover Parts

Cover Parts

Item p/n Description

1 5067-5746 Door Assembly Infinity 180 Right

2 5067-5745 Door Assembly Infinity 180 Left

3 5043-1354 Name Plate 1290 Infinity 2

4 G7104-68713 Cabinet Kit 180 Infinity II

(includes sides, bottom, top, leak adapter top and status indicator
insert)

Figure 18 Cover Parts

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 166

10 Identifying Cables

Cable Overview 168

Analog Cables 170
Remote Cables 172
CAN/LAN Cables 176
RS-232 Cable Kit 177
Agilent 1200 Module to Printer 178

This chapter provides information on cables used with the Agilent InfinityLab LC
Series modules.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 167

10 Identifying Cables
Cable Overview

Cable Overview

Never use cables other than the ones supplied by Agilent Technologies to ensure
NOTE proper functionality and compliance with safety or EMC regulations.

Analog cables
p/n Description
35900-60750 Agilent 35900A A/D converter

01046-60105 Analog cable (BNC to general purpose, spade lugs)

Remote cables
p/n Description
5188-8029 ERI
to general purpose

5188-8044 Remote Cable ERI – ERI

5188-8045 Remote Cable APG – ERI

5188-8059 ERI-Extension-Cable 1.2 m

5061-3378 Remote Cable

to 35900 A/D converter

01046-60201 Agilent module to general purpose

5188-8057 Fraction Collection ERI remote Y-cable

CAN cables
p/n Description
5181-1516 CAN cable, Agilent module to module, 0.5 m

5181-1519 CAN cable, Agilent module to module, 1 m

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 168

10 Identifying Cables
Cable Overview

LAN cables
p/n Description
5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection)

5023-0202 Twisted pair network cable, shielded, 7 m (for point to point

connection)

RS-232 cables
(not for p/n Description
FUSION board)
RS232-61601 RS-232 cable, 2.5 m
Instrument to PC, 9-to-9 pin (female). This cable has special pin-out,
and is not compatible with connecting printers and plotters. It is also
called "Null Modem Cable" with full handshaking where the wiring is
made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

5181-1561 RS-232 cable, 8 m

USB cables
p/n Description
5188-8050 USB A M-USB Mini B 3 m (PC-Module)

5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive)

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 169

10 Identifying Cables
Analog Cables

Analog Cables

One end of these cables provides a BNC connector to be connected to Agilent

modules. The other end depends on the instrument to which connection is being
made.

Agilent Module to 35900 A/D converters

p/n 35900-60750 35900 Pin Agilent Signal Name

module

1 Not connected

2 Shield Analog -

3 Center Analog +

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 170

10 Identifying Cables
Analog Cables

Agilent Module to BNC Connector

p/n 8120-1840 Pin BNC Pin Agilent Signal Name

module

Shield Shield Analog -

Center Center Analog +

Agilent Module to General Purpose

p/n 01046-60105 Pin Pin Agilent Signal Name

module

1 Not connected

2 Black Analog -

3 Red Analog +

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 171

10 Identifying Cables
Remote Cables

Remote Cables

ERI (Enhanced Remote Interface)

• 5188-8029 ERI to general purpose (D-Sub 15 pin male - open end)
• 5188-8044 ERI to ERI (D_Sub 15 pin male - male)
• 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female)

p/n 5188-8029 pin Color code Enhanced Classic Active (TTL)

Remote Remote

1 white IO1 START Low

REQUEST

2 brown IO2 STOP Low

3 green IO3 READY High

4 yellow IO4 POWER ON High

5 grey IO5 NOT USED

6 pink IO6 SHUT DOWN Low

7 blue IO7 START Low

8 red IO8 PREPARE Low

9 black 1wire DATA

10 violet DGND

11 grey-pink +5V ERI out

12 red-blue PGND

13 white-green PGND

14 brown-green +24V ERI out

15 white-yellow +24V ERI out

NC yellow-brown

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10 Identifying Cables
Remote Cables

• 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector

D_Subminiature 9 pin (APG))

p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL)

10 GND 1

1 Start Request 9 Low

2 Stop 8 Low

3 Ready 7 High

5 Power on 6 High

4 Future 5

6 Shut Down 4 Low

7 Start 3 Low

8 Prepare 2 Low

Ground Cable Shielding NC

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10 Identifying Cables
Remote Cables

• 5188-8057 ERI to APG and RJ45 (Connector D_Subminiature 15 pin (ERI),

Connector D_Subminiature 9 pin (APG), Connector plug Cat5e (RJ45))
Table 13 5188-8057 ERI to APG and RJ45

p/n 5188-8057 Pin (ERI) Signal Pin (APG) Active (TTL) Pin (RJ45)

10 GND 1 5

1 Start 9 High
Request

2 Stop 8 High

3 Ready 7 High

4 Fraction 5 High 4
Trigger

5 Power on 6 High

6 Shut Down 4 High

7 Start 3 High

8 Prepare 2 High

Ground Cable NC
Shielding

One end of these cables provides a Agilent Technologies APG (Analytical

Products Group) remote connector to be connected to Agilent modules. The
other end depends on the instrument to be connected to.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 174

10 Identifying Cables
Remote Cables

Agilent Module to Agilent 35900 A/D Converters

p/n 5061-3378 Pin 35900 A/D Pin Agilent Signal Name Active
module (TTL)

1 - White 1 - White Digital ground

2 - Brown 2 - Brown Prepare run Low

3 - Gray 3 - Gray Start Low

4 - Blue 4 - Blue Shut down Low

5 - Pink 5 - Pink Not connected

6 - Yellow 6 - Yellow Power on High

7 - Red 7 - Red Ready High

8 - Green 8 - Green Stop Low

9 - Black 9 - Black Start request Low

Agilent Module to General Purpose

p/n 01046-60201 Wire Color Pin Agilent Signal Name Active

module (TTL)

White 1 Digital ground

Brown 2 Prepare run Low

Gray 3 Start Low

Blue 4 Shut down Low

Pink 5 Not connected

Yellow 6 Power on High

Red 7 Ready High

Green 8 Stop Low

Black 9 Start request Low

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 175

10 Identifying Cables
CAN/LAN Cables

CAN/LAN Cables

Both ends of this cable provide a modular plug to be connected to

Agilent modules CAN or LAN connectors.
CAN Cables

p/n Description
5181-1516 CAN cable, Agilent module to module, 0.5 m

5181-1519 CAN cable, Agilent module to module, 1 m

LAN Cables

p/n Description
5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection)

5023-0202 Twisted pair network cable, shielded, 7 m (for point to point

connection)

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 176

10 Identifying Cables
RS-232 Cable Kit

RS-232 Cable Kit

p/n Description
RS232-61601 RS-232 cable, 2.5 m
Instrument to PC, 9-to-9 pin (female). This cable has special pin-out,
and is not compatible with connecting printers and plotters. It is also
called "Null Modem Cable" with full handshaking where the wiring is
made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

5181-1561 RS-232 cable, 8 m

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 177

10 Identifying Cables
Agilent 1200 Module to Printer

Agilent 1200 Module to Printer

To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A
socket.

p/n Description
5188-8050 USB A M-USB Mini B 3 m (PC-Module)

5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive)

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 178

11 Hardware Information

Firmware Description 180

Electrical Connections 183
Serial Number Information (ALL) 184
Rear view of the module 184
Interfaces 185
Overview Interfaces 187
ERI (Enhanced Remote Interface) 190
USB (Universal Serial Bus) 192
Setting the 6-bit Configuration Switch 193
Special Settings 195
Early Maintenance Feedback 197
Instrument Layout 198

This chapter provides detailed technical information about your binary pump.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 179

11 Hardware Information
Firmware Description

Firmware Description

The firmware of the instrument consists of two independent sections:

• a non-instrument specific section, called resident system
• an instrument specific section, called main system

Resident System
This resident section of the firmware is identical for all Agilent
1100/1200/1220/1260/1290 series modules. Its properties are:
• the complete communication capabilities (CAN, LAN, USB and RS- 232)
• memory management
• ability to update the firmware of the 'main system'

Main System
Its properties are:
• the complete communication capabilities (CAN, LAN, USB and RS- 232)
• memory management
• ability to update the firmware of the 'resident system'
In addition the main system comprises the instrument functions that are divided
into common functions like
• run synchronization through APG/ERI remote,
• error handling,
• diagnostic functions,
• or module specific functions like
• internal events such as lamp control, filter movements,
• raw data collection and conversion to absorbance.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 180

11 Hardware Information
Firmware Description

Firmware Updates
Firmware updates can be done with the Agilent Lab Advisor software with files
on the hard disk (latest version should be used).
Required tools, firmware and documentation are available from the Agilent web:
http://www.agilent.com/en-us/firmwareDownload?whid=69761
The file naming conventions are:
PPPP_RVVV_XXX.dlb, where
• PPPP is the product number, for example, 1315B for the G1315B DAD,
• R the firmware revision, for example, A for G1315B or B for the G1315C DAD,
• VVV is the revision number, for example 650 is revision 6.50,
• XXX is the build number of the firmware.
For instructions on firmware updates refer to section Replacing Firmware in
chapter "Maintenance" or use the documentation provided with the Firmware
Update Tools.

Update of main system can be done in the resident system only. Update of the
NOTE resident system can be done in the main system only.
Main and resident firmware must be from the same set.

Main FW update
Resident System Main System

Resident FW Update

Figure 19 Firmware Update Mechanism

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 181

11 Hardware Information
Firmware Description

Some modules are limited in downgrading due to their mainboard version or

NOTE their initial firmware revision. For example, a G1315C DAD SL cannot be
downgraded below firmware revision B.01.02 or to a A.xx.xx.
Some modules can be re-branded (e.g. G1314C to G1314B) to allow operation in
specific control software environments. In this case, the feature set of the target
type is used and the feature set of the original one is lost. After re-branding
(e.g. from G1314B to G1314C), the original feature set is available again.
All this specific information is described in the documentation provided with the
firmware update tools.

The firmware update tools, firmware and documentation are available from the
Agilent web.
• http://www.agilent.com/en-us/firmwareDownload?whid=69761

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 182

11 Hardware Information
Electrical Connections

Electrical Connections

• The CAN bus is a serial bus with high-speed data transfer. The two
connectors for the CAN bus are used for internal module data transfer and
synchronization.
• The ERI/REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shutdown, prepare, and so on.
• With the appropriate software, the LAN connector may be used to control the
module from a computer through a LAN connection. This connector is
activated and can be configured with the configuration switch.
• With the appropriate software, the USB connector may be used to control the
module from a computer through a USB connection.
• The power input socket accepts a line voltage of 100 – 240 VAC ± 10 % with a
line frequency of 50 or 60 Hz. Maximum power consumption varies by
module. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible fuses
because automatic electronic fuses are implemented in the power supply.

Never use cables other than the ones supplied by Agilent Technologies to ensure
NOTE proper functionality and compliance with safety or EMC regulations.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 183

11 Hardware Information
Electrical Connections

Serial Number Information (ALL)

The serial number information on the instrument labels provide the following
information:

CCXZZ00000 Format

CC Country of manufacturing
• DE = Germany
• JP = Japan
• CN = China

X Alphabetic character A-Z (used by manufacturing)

ZZ Alpha-numeric code 0-9, A-Z, where each combination

unambiguously denotes a module (there can be more than one
code for the same module)

00000 Serial number

Rear view of the module

Configuration switch

USB-Mini-Port

LAN

ERI

CAN

Power socket

Figure 20 Rear view of the pump – electrical connections and label

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 184

11 Hardware Information
Interfaces

Interfaces

The Agilent InfinityLab LC Series modules provide the following interfaces:

Table 14 Agilent InfinityLab LC Series Interfaces

Module CAN USB LAN RS-232 Analog APG (A) Special

(on-board) / ERI (E)

Pumps

G7104A/C 2 No Yes Yes 1 A

G7110B 2 Yes Yes No No E

G7111A/B, G5654A 2 Yes Yes No No E

G7112B 2 Yes Yes No No E

G7120A, G7132A 2 No Yes Yes 1 A

G7161A/B 2 Yes Yes No No E

Samplers

G7129A/B/C 2 Yes Yes No No E

G7167A/B, G7137A, G5668A, 2 Yes Yes No No E

G3167A

G7157A 2 Yes Yes No No E

Detectors

G7114A/B 2 Yes Yes No 1 E

G7115A 2 Yes Yes No 1 E

G7117A/B/C 2 Yes Yes No 1 E

G7121A/B 2 Yes Yes No 1 E

G7162A/B 2 Yes Yes No 1 E

G7165A 2 Yes Yes No 1 E

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 185

11 Hardware Information
Interfaces

Table 14 Agilent InfinityLab LC Series Interfaces

Module CAN USB LAN RS-232 Analog APG (A) Special

(on-board) / ERI (E)

Fraction Collectors

G7158B 2 Yes Yes No No E

G7159B 2 Yes Yes No No E

G7166A 2 No No No No No Requires a host module

with on-board LAN with
minimum FW B.06.40 or
C.06.40, or with additional
G1369C LAN Card

G1364E/F, G5664B 2 Yes Yes No No E THERMOSTAT for

G1330B

Others

G1170A 2 No No No No No

G7116A/B 2 No No No No No Requires a host module

with on-board LAN or with
additional G1369C LAN
Card.

G7122A No No No Yes No A

G7170B 2 No No No No No Requires a host module

with on-board LAN with
minimum FW B.06.40 or
C.06.40, or with additional
G1369C LAN Card

The detector (DAD/MWD/FLD/VWD/RID) is the preferred access point for

NOTE control via LAN. The inter-module communication is done via CAN.

• CAN connectors as interface to other modules

• LAN connector as interface to the control software
• RS-232C as interface to a computer
• USB (Universal Series Bus) as interface to a computer
• REMOTE connector as interface to other Agilent products
• Analog output connector(s) for signal output

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11 Hardware Information
Interfaces

Overview Interfaces
CAN
The CAN is inter-module communication interface. It is a 2-wire serial bus
system supporting high speed data communication and real-time requirement.

LAN
The modules have either an interface slot for a LAN card (e.g. Agilent G1369B/C
LAN Interface) or they have an on-board LAN interface (e.g. detectors G1315C/D
DAD and G1365C/D MWD). This interface allows the control of the
module/system via a PC with the appropriate control software. Some modules
have neither on-board LAN nor an interface slot for a LAN card (e.g. G1170A
Valve Drive or G4227A Flexible Cube). These are hosted modules and require a
Host module with firmware B.06.40 or later or with additional G1369C LAN Card.

If an Agilent detector (DAD/MWD/FLD/VWD/RID) is in the system, the LAN

NOTE should be connected to the DAD/MWD/FLD/VWD/RID (due to higher data load).
If no Agilent detector is part of the system, the LAN interface should be installed
in the pump or autosampler.

USB
The USB interface replaces the RS-232 Serial interface in new FUSION generation
modules. For details on USB refer to “USB (Universal Serial Bus)” on page 192.

Analog Signal Output

The analog signal output can be distributed to a recording device. For details
refer to the description of the module’s mainboard.

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11 Hardware Information
Interfaces

Remote (ERI)
The ERI (Enhanced Remote Interface) connector may be used in combination
with other analytical instruments from Agilent Technologies if you want to use
features as common shut down, prepare, and so on.
It allows easy connection between single instruments or systems to ensure
coordinated analysis with simple coupling requirements.
The subminiature D connector is used. The module provides one remote
connector which is inputs/outputs (wired- or technique).
To provide maximum safety within a distributed analysis system, one line is
dedicated to SHUT DOWN the system’s critical parts in case any module detects
a serious problem. To detect whether all participating modules are switched on
or properly powered, one line is defined to summarize the POWER ON state of all
connected modules. Control of analysis is maintained by signal readiness READY
for next analysis, followed by START of run and optional STOP of run triggered on
the respective lines. In addition PREPARE and START REQUEST may be issued.
The signal levels are defined as:
• standard TTL levels (0 V is logic true, + 5.0 V is false),
• fan-out is 10,
• input load is 2.2 kOhm against + 5.0 V, and
• output are open collector type, inputs/outputs (wired- or technique).

All common TTL circuits operate with a 5 V power supply. A TTL signal is defined
NOTE as "low" or L when between 0 V and 0.8 V and "high" or H when between 2.0 V
and 5.0 V (with respect to the ground terminal).

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11 Hardware Information
Interfaces

Table 15 ERI signal distribution

Pin Signal Description

1 START REQUEST (L) Request to start injection cycle (for example, by start key on any
module). Receiver is the autosampler.

2 STOP (L) Request to reach system ready state as soon as possible (for
example, stop run, abort or finish and stop injection). Receiver is any
module performing run-time controlled activities.

3 READY (H) System is ready for next analysis. Receiver is any sequence
controller.

4 POWER ON (H) All modules connected to system are switched on. Receiver is
any module relying on operation of others.

5 Not used

6 SHUT DOWN (L) System has serious problem (for example, leak: stops pump).
Receiver is any module capable to reduce safety risk.

7 START (L) Request to start run / timetable. Receiver is any module

performing run-time controlled activities.

8 PREPARE (L) Request to prepare for analysis (for example, calibration,

detector lamp on). Receiver is any module performing pre-analysis
activities.

Special Interfaces
There is no special interface for this module.

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11 Hardware Information
Interfaces

ERI (Enhanced Remote Interface)

ERI replaces the AGP Remote Interface that is used in the HP
1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity
HPLC modules. All new InfinityLab LC Series products using the FUSION core
electronics use ERI. This interface is already used in the Agilent Universal
Interface Box 2 (UIB2)

ERI Description
The ERI interface contains eight individual programmable input/output pins. In
addition, it provides 24 V power and 5 V power and a serial data line to detect and
recognize further add-ons that could be connected to this interface. This way the
interface can support various additional devices like sensors, triggers (in and out)
and small controllers, etc.

ERI

Figure 21 Location of the ERI interface (example shows a G7114A/B VWD)

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11 Hardware Information
Interfaces

Pin Enhanced Remote

1 IO 1 (START
REQUEST)

2 IO 2 (STOP)

3 IO 3 (READY)

4 IO 4 (POWER ON)

5 IO 5 (NOT USED)

6 IO 6 (SHUT DOWN)

7 IO 7 (START)

8 IO 8 (PREPARE)

9 1 wire DATA

10 DGND

11 +5 V ERI out

12 PGND

13 PGND

14 +24 V ERI out

15 +24 V ERI out

IO (Input/Output) Lines
• Eight generic bi-directional channels (input or output).
• Same as the APG Remote.
• Devices like valves, relays, ADCs, DACs, controllers can be
supported/controlled.

1-Wire Data (Future Use)

This serial line can be used to read out an EPROM or write into an EPROM of a
connected ERI-device. The firmware can detect the connected type of device
automatically and update information in the device (if required).

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11 Hardware Information
Interfaces

5V Distribution (Future Use)

• Available directly after turning on the hosting module (assures that the
firmware can detect certain basic functionality of the device).
• For digital circuits or similar.
• Provides 500 mA maximum.
• Short-circuit proof with automatic switch off (by firmware).

24V Distribution (Future Use)

• Available by firmware command (defined turn on/off).
• For devices that need higher power
• Class 0: 0.5 A maximum (12 W)
• Class 1: 1.0 A maximum (24 W)
• Class 2: 2.0 A maximum (48 W)
• Class depends on hosting module’s internal power overhead.
• If a connected device requires more power the firmware detects this
(overcurrent detection) and provides the information to the user interface.
• Fuse used for safety protection (on board).
• Short circuit will be detected through hardware.

USB (Universal Serial Bus)

USB (Universal Serial Bus) - replaces RS232, supports:
• a PC with control software (for example Agilent Lab Advisor)
• USB Flash Disk

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11 Hardware Information
Setting the 6-bit Configuration Switch

Setting the 6-bit Configuration Switch

The 6-bit configuration switch is located at the rear of the module with FUSION
electronics. Switch settings provide configuration parameters for LAN and
instrument specific initialization procedures.
All modules with FUSION electronics:
• Default is ALL switches DOWN (best settings).
• Default IP address for LAN 192.168.254.11
• For specific LAN modes switches 4-5 must be set as required.
• For boot resident/cold start modes switches 1+2 or 6 must be UP.

Configuration switch

Figure 22 Location of Configuration switch (example shows a G7114A/B VWD)

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11 Hardware Information
Setting the 6-bit Configuration Switch

Table 16 6-bit Configuration Switch

Mode Function/Setting

Switch 1 Switch 2 Switch 3 Switch 4 Switch 5 Switch 6

COM1 0 n.a.2 n.a. LAN Init Mode n.a.

Use Default IP Address3 0 0 0 0 0

Use Stored IP Address 0 0 0 1 0

Use DHCP to request IP Address4 0 0 1 0 0

Test 1 System n.a. n.a. n.a. ColdStart

Boot Main System / Keep Data 0 0 0 0 0

Boot Resident System / Keep Data 1 0 0 0 0

Boot Main System / Revert to

0 0 0 0 1
Default Data

Boot Resident System / Revert to

1 0 0 0 1
Default Data

1 When selecting mode COM, settings are stored to non-volatile memory. When selecting mode TEST, COM settings are taken from
non-volatile memory.

2 not assigned - Always keep these switches on position ‘0’ (off)

3 Default IP Address is 192.168.254.11

4 Host Name will be the MAC address.

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11 Hardware Information
Setting the 6-bit Configuration Switch

Special Settings
Boot-Resident/Main
Firmware update procedures may require this mode in case of firmware loading
errors (main/resident firmware part).
If you use the following switch settings and power the instrument up again, the
instrument firmware stays in the resident/main mode. In resident mode, it is not
operable as a module. It only uses basic functions of the operating system for
example, for communication. In this mode the main firmware can be loaded
(using update utilities).

Forced Cold Start

A forced cold start can be used to bring the module into a defined mode with
default parameter settings.
• Boot Main System / Revert to Default Data
The instrument will boot to main mode and changes to the module’s default
parameter. May be also required to load resident firmware into the module.
• Boot Resident System / Revert to Default Data
The instrument will boot to resident mode and changes to the module’s
default parameter. May be also required to load main firmware into the
module.

Loss of data
C AU T I O N
Forced cold start erases all methods and data stored in the non-volatile
memory. Exceptions are calibration settings, diagnosis and repair log books
which will not be erased.
 Save your methods and data before executing a forced cold start.

If you use the following switch settings and power the instrument up again, it will
start as described above.

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11 Hardware Information
Setting the 6-bit Configuration Switch

Table 17 Boot Resident / Forced Coldstart

SW1 SW2 SW3 SW4 SW5 SW6 Init Mode

1 0 0 0 0 0 Boot Main System / Keep Data

1 1 0 0 0 0 Boot Resident System / Keep Data

1 0 0 0 0 1 Boot Main System / Revert to Default Data

1 1 0 0 0 1 Boot Resident System / Revert to Default Data

Note: The setting '0' (down) is essential.

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11 Hardware Information
Early Maintenance Feedback

Early Maintenance Feedback

Maintenance requires the exchange of components that are subject to wear or

stress. Ideally, the frequency at which components are exchanged should be
based on the intensity of use of the module and the analytical conditions, and not
on a predefined time interval. The early maintenance feedback (EMF) feature
monitors the use of specific components in the instrument, and provides
feedback when the user-selectable limits have been exceeded. The visual
feedback in the user interface provides an indication that maintenance
procedures should be scheduled.

EMF Counters
EMF counters increment with use and can be assigned a maximum limit which
provides visual feedback in the user interface when the limit is exceeded. Some
counters can be reset to zero after the required maintenance procedure.

Using the EMF Counters

The user-settable EMF limits for the EMF Counters enable the early maintenance
feedback to be adapted to specific user requirements. The useful maintenance
cycle is dependent on the requirements for use. Therefore, the definition of the
maximum limits need to be determined based on the specific operating
conditions of the instrument.

Setting the EMF Limits

The setting of the EMF limits must be optimized over one or two maintenance
cycles. Initially the default EMF limits should be set. When instrument
performance indicates maintenance is necessary, take note of the values
displayed by the EMF counters. Enter these values (or values slightly less than the
displayed values) as EMF limits, and then reset the EMF counters to zero. The
next time the EMF counters exceed the new EMF limits, the EMF flag will be
displayed, providing a reminder that maintenance needs to be scheduled.

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11 Hardware Information
Instrument Layout

Instrument Layout

The industrial design of the module incorporates several innovative features. It

uses Agilent’s E-PAC concept for the packaging of electronics and mechanical
assemblies. This concept is based upon the use of expanded polypropylene
(EPP) layers of foam plastic spacers in which the mechanical and electronic
boards components of the module are placed. This pack is then housed in a
metal inner cabinet which is enclosed by a plastic external cabinet. The
advantages of this packaging technology are:
• virtual elimination of fixing screws, bolts or ties, reducing the number of
components and increasing the speed of assembly/disassembly,
• the plastic layers have air channels molded into them so that cooling air can
be guided exactly to the required locations,
• the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
• the metal inner cabinet shields the internal electronics from electromagnetic
interference and also helps to reduce or eliminate radio frequency emissions
from the instrument itself.

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12 LAN Configuration

What You Have to Do First 200

TCP/IP parameter configuration 201
Configuration Switches 202
Initialization Mode Selection 203
Dynamic Host Configuration Protocol (DHCP) 205
General Information (DHCP) 205
Setup (DHCP) 206
Manual Configuration 208
With Telnet 209

This chapter provides information on connecting the module to the Agilent

ChemStation PC.

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12 LAN Configuration
What You Have to Do First

What You Have to Do First

The module has an on- board LAN communication interface.

This chapter is generic and may show figures that differ from your module. The
NOTE functionality is the same.

1 Note the MAC (Media Access Control) address for further reference. The MAC
or hardware address of the LAN interfaces is a world wide unique identifier.
No other network device will have the same hardware address. The MAC
address can be found on a label at the rear of the module underneath the
configuration switch (see Figure 24 on page 200).

Part number of the detector main board

Revision Code, Vendor, Year and Week of assembly
MAC address
Country of Origin

Figure 23 MAC-Label

2 Connect the instrument's LAN interface (see Figure 24 on page 200) to

• the PC network card using a crossover network cable (point-to-point) or
• a hub or switch using a standard LAN cable.

LAN interface

MAC label

Figure 24 Location of LAN interfaces and MAC label

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12 LAN Configuration
TCP/IP parameter configuration

TCP/IP parameter configuration

To operate properly in a network environment, the LAN interface must be

configured with valid TCP/IP network parameters. These parameters are:
• IP address
• Subnet Mask
• Default Gateway
The TCP/IP parameters can be configured by the following methods:
• by automatically requesting the parameters from a network-based DHCP
Server (using the so-called Dynamic Host Configuration Protocol). This mode
requires a LAN-onboard Module or a G1369C LAN Interface card, see “Setup
(DHCP)” on page 206
• by manually setting the parameters using Telnet
• by manually setting the parameters using the Local Controller
The LAN interface differentiates between several initialization modes. The
initialization mode (short form ‘init mode’) defines how to determine the active
TCP/IP parameters after power-on. The parameters may be derived non-volatile
memory or initialized with known default values. The initialization mode is
selected by the configuration switch, see Table 18 on page 203.

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12 LAN Configuration
Configuration Switches

Configuration Switches

The configuration switch can be accessed at the rear of the module.

Configuration switch

Figure 25 Location of Configuration switch (example shows a G7114A/B VWD)

The module is shipped with all switches set to OFF, as shown above.

To perform any LAN configuration, SW1 and SW2 must be set to OFF.
NOTE

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12 LAN Configuration
Initialization Mode Selection

Initialization Mode Selection

The following initialization (init) modes are selectable:

Table 18 Initialization Mode Switches

SW1 SW2 SW3 SW4 SW5 SW6 Init Mode

0 0 0 0 0 0 Use Default IP Address

0 0 0 0 1 0 Use Stored IP Address

0 0 0 1 0 0 Use DHCP

Note: The setting ‘0’ (down) is essential.

Default IP address for LAN is 192.168.254.11.

DHCP address is the module’s LAN MAC address.

Using Stored
When initialization mode Using Stored is selected, the parameters are taken from
the non-volatile memory of the module. The TCP/IP connection will be
established using these parameters. The parameters were configured previously
by one of the described methods.

Non-Volatile
RAM
Active
Parameter
Stored
Parameter

Figure 26 Using Stored (Principle)

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12 LAN Configuration
Initialization Mode Selection

Using Default
When Using Default is selected, the factory default parameters are taken instead.
These parameters enable a TCP/IP connection to the LAN interface without
further configuration, see Table 19 on page 204.

Default Active
Parameter Parameter

Figure 27 Using Default (Principle)

Using the default address in your local area network may result in network
NOTE problems. Take care and change it to a valid address immediately.

Table 19 Using Default Parameters

IP address: 192.168.254.11

Subnet Mask: 255.255.255.0

Default Gateway not specified

Since the default IP address is a so-called local address, it will not be routed by
any network device. Thus, the PC and the module must reside in the same
subnet.
The user may open a Telnet session using the default IP address and change the
parameters stored in the non-volatile memory of the module. He may then close
the session, select the initialization mode Using Stored, power-on again and
establish the TCP/IP connection using the new parameters.
When the module is wired to the PC directly (e.g. using a cross-over cable or a
local hub), separated from the local area network, the user may simply keep the
default parameters to establish the TCP/IP connection.

In the Using Default mode, the parameters stored in the memory of the module
NOTE are not cleared automatically. If not changed by the user, they are still available,
when switching back to the mode Using Stored.

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12 LAN Configuration
Dynamic Host Configuration Protocol (DHCP)

Dynamic Host Configuration Protocol (DHCP)

General Information (DHCP)

The Dynamic Host Configuration Protocol (DHCP) is an auto configuration
protocol used on IP networks. The DHCP functionality is available on all Agilent
HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and
“B”-firmware (B.06.40 or above) or modules with "D"-firmware. All modules
should use latest firmware from the same set.
When the initialization mode “DHCP” is selected, the card tries to download the
parameters from a DHCP Server. The parameters obtained become the active
parameters immediately. They are not stored to the non-volatile memory of the
card.
Besides requesting the network parameters, the card also submits its hostname
to the DHCP Server. The hostname equals the MAC address of the card, e.g.
0030d3177321. It is the DHCP server's responsibility to forward the
hostname/address information to the Domain Name Server. The card does not
offer any services for hostname resolution (e.g. NetBIOS).

DHCP Active
Server Parameter

Figure 28 DHCP (Principle)

1 It may take some time until the DHCP server has updated the DNS server with
NOTE the hostname information.
2 It may be necessary to fully qualify the hostname with the DNS suffix, e.g.
0030d3177321.country.company.com.
3 The DHCP server may reject the hostname proposed by the card and assign a
name following local naming conventions.

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12 LAN Configuration
Dynamic Host Configuration Protocol (DHCP)

Setup (DHCP)

The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN
Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware. All
modules should use latest firmware from the same set.

1 Note the MAC address of the LAN interface (provided with G1369C LAN
Interface Card or mainboard). This MAC address is on a label on the card or at
the rear of the mainboard, for example, 0030d3177321.
On the Local Controller the MAC address can be found under Details in the
LAN section.

Figure 29 LAN Setting on Instant Pilot

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12 LAN Configuration
Dynamic Host Configuration Protocol (DHCP)

2 Set the configuration switch to DHCP either on the G1369C LAN Interface
Card or the mainboard of above mentioned modules.
Table 20 G1369C LAN Interface Card (configuration switch on the card)

SW 4 SW 5 SW 6 SW 7 SW 8 Initialization Mode

ON OFF OFF OFF OFF DHCP

Table 21 LC Modules with 8-bit configuration switch (B-firmware) (configuration switch at rear
of the instrument)

SW 6 SW 7 SW 8 Initialization Mode

ON OFF OFF DHCP

3 Turn on the module that hosts the LAN interface.

4 Configure your Control Software (e.g. OpenLAB CDS ChemStation Edition, Lab
Advisor, Firmware Update Tool) and use MAC address as host name, e.g.
0030d3177321.
The LC system should become visible in the control software (see Note in
section “General Information (DHCP)” on page 205).

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12 LAN Configuration
Manual Configuration

Manual Configuration

Manual configuration only alters the set of parameters stored in the non-volatile
memory of the module. It never affects the currently active parameters.
Therefore, manual configuration can be done at any time. A power cycle is
mandatory to make the stored parameters become the active parameters, given
that the initialization mode selection switches are allowing it.

TELNET
Session
Non-Volatile
RAM

Stored
Parameter

Control
Module

Figure 30 Manual Configuration (Principle)

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12 LAN Configuration
Manual Configuration

With Telnet
Whenever a TCP/IP connection to the module is possible (TCP/IP parameters set
by any method), the parameters may be altered by opening a Telnet session.
1 Open the system (DOS) prompt window by clicking on Windows START
button and select “Run...”. Type “cmd” and press OK.
2 Type the following at the system (DOS) prompt:
• c:\>telnet <IP address> or
• c:\>telnet <host name>

Figure 31 Telnet - Starting a session

where <IP address> may be the assigned address from a Bootp cycle, a
configuration session with the Handheld Controller, or the default IP address
(see “Configuration Switches” on page 202).
When the connection was established successfully, the module responds
with the following:

Figure 32 A connection to the module is made

3 Type
? and press enter to see the available commands.

Figure 33 Telnet Commands

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12 LAN Configuration
Manual Configuration

Table 22 Telnet Commands

Value Description

? displays syntax and descriptions of commands

/ displays current LAN settings

ip <x.x.x.x> sets new ip address

sm <x.x.x.x> sets new subnet mask

gw <x.x.x.x> sets new default gateway

exit exits shell and saves all changes

4 To change a parameter follows the style:

• parameter value, for example:
ip 134.40.28.56
Then press [Enter], where parameter refers to the configuration parameter
you are defining, and value refers to the definitions you are assigning to that
parameter. Each parameter entry is followed by a carriage return.
5 Use the “/” and press Enter to list the current settings.

information about the LAN interface

MAC address, initialization mode
Initialization mode is Using Stored
active TCP/IP settings

TCP/IP status - here ready

Figure 34 Telnet - Current settings in "Using Stored" mode connected to PC with controller software (e.g. Agilent
ChemStation), here not connected

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12 LAN Configuration
Manual Configuration

6 Change the IP address (in this example 192.168.254.12) and type “/” to list
current settings.

change of IP setting to
Initialization mode is Using Stored

active TCP/IP settings

stored TCP/IP settings in non-volatile memory

connected to PC with controller software (e.g. Agilent

ChemStation), here not connected

Figure 35 Telnet - Change IP settings

7 When you have finished typing the configuration parameters, type

exit and press Enter to exit with storing parameters.

Figure 36 Closing the Telnet Session

If the Initialization Mode Switch is changed now to “Using Stored” mode, the
NOTE instrument will take the stored settings when the module is re-booted. In the
example above it would be 192.168.254.12.

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13 Appendix

General Safety Information 213

General Safety Information 213
Safety Standards 213
General 213
Before Applying Power 214
Ground the Instrument 214
Do Not Operate in an Explosive Atmosphere 215
Do Not Remove the Instrument Cover 215
Do Not Modify the Instrument 215
In Case of Damage 215
Solvents 216
Safety Symbols 217
Waste Electrical and Electronic Equipment (WEEE) Directive 219
Radio Interference 220
Sound Emission 221
Agilent Technologies on the Internet 222

This chapter provides addition information on safety, legal and web.

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13 Appendix
General Safety Information

General Safety Information

General Safety Information

The following general safety precautions must be observed during all phases of
operation, service, and repair of this instrument. Failure to comply with these
precautions or with specific warnings elsewhere in this manual violates safety
standards of design, manufacture, and intended use of the instrument. Agilent
Technologies assumes no liability for the customer’s failure to comply with these
requirements.

Ensure the proper usage of the equipment.

WAR N IN G
The protection provided by the equipment may be impaired.
 The operator of this instrument is advised to use the equipment in a
manner as specified in this manual.

Safety Standards
This is a Safety Class I instrument (provided with terminal for protective earthing)
and has been manufactured and tested according to international safety
standards.

General
Do not use this product in any manner not specified by the manufacturer. The
protective features of this product may be impaired if it is used in a manner not
specified in the operation instructions.

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13 Appendix
General Safety Information

Before Applying Power

Wrong voltage range, frequency or cabling
WAR N IN G
Personal injury or damage to the instrument
 Verify that the voltage range and frequency of your power distribution
matches to the power specification of the individual instrument.
 Never use cables other than the ones supplied by Agilent Technologies
to ensure proper functionality and compliance with safety or EMC
regulations.
 Make all connections to the unit before applying power.

Note the instrument's external markings described under “Safety Symbols” on

NOTE page 217.

Ground the Instrument

Missing electrical ground
WAR N IN G
Electrical shock
 If your product is provided with a grounding type power plug, the
instrument chassis and cover must be connected to an electrical ground
to minimize shock hazard.
 The ground pin must be firmly connected to an electrical ground (safety
ground) terminal at the power outlet. Any interruption of the protective
(grounding) conductor or disconnection of the protective earth terminal
will cause a potential shock hazard that could result in personal injury.

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13 Appendix
General Safety Information

Do Not Operate in an Explosive Atmosphere

Presence of flammable gases or fumes
WAR N IN G
Explosion hazard
 Do not operate the instrument in the presence of flammable gases or
fumes.

Do Not Remove the Instrument Cover

Instrument covers removed
WAR N IN G
Electrical shock
 Do Not Remove the Instrument Cover
 Only Agilent authorized personnel are allowed to remove instrument
covers. Always disconnect the power cables and any external circuits
before removing the instrument cover.

Do Not Modify the Instrument

Do not install substitute parts or perform any unauthorized modification to the
product. Return the product to an Agilent Sales and Service Office for service and
repair to ensure that safety features are maintained.

In Case of Damage
Damage to the module
WAR N IN G
Personal injury (for example electrical shock, intoxication)
 Instruments that appear damaged or defective should be made
inoperative and secured against unintended operation until they can be
repaired by qualified service personnel.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 215

13 Appendix
General Safety Information

Solvents
Toxic, flammable and hazardous solvents, samples and reagents
WAR N IN G
The handling of solvents, samples and reagents can hold health and safety
risks.
 When working with these substances observe appropriate safety
procedures (for example by wearing goggles, safety gloves and
protective clothing) as described in the material handling and safety
data sheet supplied by the vendor, and follow good laboratory practice.
 Do not use solvents with an auto-ignition temperature below 200 °C
(392 °F). Do not use solvents with a boiling point below 56 °C (133 °F).
 Avoid high vapor concentrations. Keep the solvent temperature at least
40 °C (72 °F) below the boiling point of the solvent used. This includes
the solvent temperature in the sample compartment. For the solvents
methanol and ethanol keep the solvent temperature at least 25 °C
(45 °F) below the boiling point.
 Do not operate the instrument in an explosive atmosphere.
 Do not use solvents of ignition Class IIC according IEC 60079-20-1
(for example, carbon disulfide).
 Reduce the volume of substances to the minimum required for the
analysis.
 Never exceed the maximum permissible volume of solvents (8 L) in the
solvent cabinet. Do not use bottles that exceed the maximum
permissible volume as specified in the usage guideline for solvent
cabinet.
 Ground the waste container.
 Regularly check the filling level of the waste container. The residual free
volume in the waste container must be large enough to collect the waste
liquid.
 To achieve maximal safety, regularly check the tubing for correct
installation.

For details, see the usage guideline for the solvent cabinet. A printed copy of the
NOTE guideline has been shipped with the solvent cabinet, electronic copies are
available in the Agilent Information Center or via the Internet.

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13 Appendix
General Safety Information

Safety Symbols
Table 23 Symbols

The apparatus is marked with this symbol when the user shall refer to the
instruction manual in order to protect risk of harm to the operator and to
protect the apparatus against damage.

Indicates dangerous voltages.

Indicates a protected ground terminal.

The apparatus is marked with this symbol when hot surfaces are available
and the user should not touch it when heated up.

Sample Cooler unit is designed as vapor-compression refrigeration system.

Contains fluorinated greenhouse gas (refrigerant) according to the Kyoto
protocol.
For specifications of refrigerant, charge capacity, carbon dioxide equivalent
(CDE), and global warming potential (GWP) see instrument label.

Flammable Material
For Sample Thermostat which uses flammable refrigerant consult Agilent
Information Center / User Manual before attempting to install or service this
equipment. All safety precautions must be followed.

Confirms that a manufactured product complies with all applicable

European Community directives. The European Declaration of Conformity is
available at:
http://regulations.corporate.agilent.com/DoC/search.htm

Manufacturing date.

Power symbol indicates On/Off.

The apparatus is not completely disconnected from the mains supply when
the power switch is in the Off position

Pacemaker
Magnets could affect the functioning of pacemakers and implanted heart
defibrillators.
A pacemaker could switch into test mode and cause illness. A heart
defibrillator may stop working. If you wear these devices keep at least
55 mm distance to magnets. Warn others who wear these devices from
getting too close to magnets.

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13 Appendix
General Safety Information

Table 23 Symbols

Magnetic field
Magnets produce a far-reaching, strong magnetic field. They could damage
TVs and laptops, computer hard drives, credit and ATM cards, data storage
media, mechanical watches, hearing aids and speakers. Keep magnets at
least 25 mm away from devices and objects that could be damaged by
strong magnetic fields.

Indicates a pinching or crushing hazard

Indicates a piercing or cutting hazard.

A WARNING
WAR N IN G
alerts you to situations that could cause physical injury or death.
 Do not proceed beyond a warning until you have fully understood and
met the indicated conditions.

A CAUTION
C AU T I O N
alerts you to situations that could cause loss of data, or damage of equipment.
 Do not proceed beyond a caution until you have fully understood and met
the indicated conditions.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 218

13 Appendix
Waste Electrical and Electronic Equipment (WEEE) Directive

Waste Electrical and Electronic Equipment

(WEEE) Directive

This product complies with the European WEEE Directive marking requirements.
The affixed label indicates that you must not discard this electrical/electronic
product in domestic household waste.

Do not dispose of in domestic household waste

NOTE
To return unwanted products, contact your local Agilent office, or see
http://www.agilent.com for more information.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 219

13 Appendix
Radio Interference

Radio Interference

Cables supplied by Agilent Technologies are screened to provide optimized

protection against radio interference. All cables are in compliance with safety or
EMC regulations.

Test and Measurement

If test and measurement equipment is operated with unscreened cables, or used
for measurements on open set-ups, the user has to assure that under operating
conditions the radio interference limits are still met within the premises.

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 220

13 Appendix
Sound Emission

Sound Emission

Sound pressure
Sound pressure Lp <70 db(A) according to DIN EN ISO 7779

Schalldruckpegel
Schalldruckpegel Lp <70 db(A) nach DIN EN ISO 7779

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 221

13 Appendix
Agilent Technologies on the Internet

Agilent Technologies on the Internet

For the latest information on products and services visit our worldwide web site
on the Internet at:
http://www.agilent.com

InfinityLab LC Series 1260 Infinity II Binary Pump User Manual 222

In This Book

This manual contains technical reference

information about the Agilent 1260 Infinity II
Binary Pump G7112B. The manual describes
the following:
• introduction,
• site requirements and specifications,
• using the binary pump,
• optimizing performance,
• troubleshooting and diagnostics,
• maintenance,
• parts and materials for maintenance,
• identifying cables,
• hardware information,
• appendix.

www.agilent.com
Agilent Technologies Inc. 2016-2021
Edition: 10/2021
Document No: SD-29000214 Rev. C