SAFETY DATA SHEET

according to Regulation UK SI 2019/758 and UK SI 2020/1577 as amended

Creation Date 05-May-2010 Revision Date 09-Feb-2024 Revision Number 7

SECTION 1: IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE

COMPANY/UNDERTAKING

1.1. Product identifier

Product Description: Benedict's reagent (qualitative)

Cat No. : J/2100/17, J/2100/15

1.2. Relevant identified uses of the substance or mixture and uses advised against

Recommended Use Laboratory chemicals.

Uses advised against No Information available

1.3. Details of the supplier of the safety data sheet

Company .
UK entity/business name
Fisher Scientific UK
Bishop Meadow Road, Loughborough,
Leicestershire LE11 5RG, United Kingdom

EU entity/business name
Thermo Fisher Scientific
Janssen Pharmaceuticalaan 3a
2440 Geel, Belgium

E-mail address [email protected]

1.4. Emergency telephone number

Tel: 01509 231166
Chemtrec US: (800) 424-9300
Chemtrec EU: 001-703-527-3887

SECTION 2: HAZARDS IDENTIFICATION

2.1. Classification of the substance or mixture

CLP Classification - According to GB-CLP Regulations UK SI 2019/720 and UK SI 2020/1567

Physical hazards

Based on available data, the classification criteria are not met

Health hazards

Serious Eye Damage/Eye Irritation Category 2 (H319)

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FSUJ2100
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Environmental hazards

Chronic aquatic toxicity Category 3 (H412)

Full text of Hazard Statements: see section 16

2.2. Label elements

Signal Word Warning

Hazard Statements
H319 - Causes serious eye irritation
H412 - Harmful to aquatic life with long lasting effects

Precautionary Statements
P273 - Avoid release to the environment
P280 - Wear protective gloves/protective clothing/eye protection/face protection
P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and
easy to do. Continue rinsing

2.3. Other hazards

This product does not contain any known or suspected endocrine disruptors

SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTS

3.2. Mixtures

Component CAS No EC No Weight % CLP Classification - According to

GB-CLP Regulations UK SI 2019/720 and
UK SI 2020/1567
Sodium citrate 68-04-2 200-675-3 15 -
Sodium carbonate 497-19-8 207-838-8 13 Eye Irrit. 2 (H319)
Copper (II) sulfate pentahydrate (1:1:5) 7758-99-8 1.6 Acute Tox. 4 (H302)
Eye Dam. 1 (H318)
Aquatic Acute 1 (H400)
Aquatic Chronic 1 (H410)
Sodium bicarbonate 144-55-8 205-633-8 1 -
Water 7732-18-5 231-791-2 69.4 -

Component Specific concentration limits M-Factor Component notes

(SCL's)
Copper (II) sulfate pentahydrate (1:1:5) - 10 (Acute) -
1 (Chronic)

Component ECHA (RAC) ATE (Oral) ECHA (RAC) ATE (Dermal) ECHA (RAC) ATE (Inhalation)
Copper (II) sulfate pentahydrate (1:1:5) ATE = 481 mg/kg bw - -

Components Reach Registration Number

Sodium citrate 01-2119457027-40
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Copper (II) sulfate pentahydrate 01-2119520566-40 (for the anhydrous form)
Sodium bicarbonate 01-2119457606-32
Sodium carbonate 01-2119485498-19

Full text of Hazard Statements: see section 16

SECTION 4: FIRST AID MEASURES

4.1. Description of first aid measures

Eye Contact Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Get
medical attention.

Skin Contact Wash off immediately with plenty of water for at least 15 minutes. Get medical attention
immediately if symptoms occur.

Ingestion Do NOT induce vomiting. Get medical attention.

Inhalation Remove to fresh air. Get medical attention immediately if symptoms occur. If not breathing,
give artificial respiration.

Self-Protection of the First Aider Ensure that medical personnel are aware of the material(s) involved, take precautions to
protect themselves and prevent spread of contamination.

4.2. Most important symptoms and effects, both acute and delayed

No information available.

4.3. Indication of any immediate medical attention and special treatment needed

Notes to Physician Treat symptomatically.

SECTION 5: FIREFIGHTING MEASURES

5.1. Extinguishing media

Suitable Extinguishing Media

Substance is nonflammable; use agent most appropriate to extinguish surrounding fire.

Extinguishing media which must not be used for safety reasons

No information available.

5.2. Special hazards arising from the substance or mixture

Thermal decomposition can lead to release of irritating gases and vapors.

Hazardous Combustion Products

Thermal decomposition can lead to release of irritating gases and vapors.

5.3. Advice for firefighters

As in any fire, wear self-contained breathing apparatus pressure-demand, MSHA/NIOSH (approved or equivalent) and full
protective gear.

SECTION 6: ACCIDENTAL RELEASE MEASURES

6.1. Personal precautions, protective equipment and emergency procedures

Ensure adequate ventilation. Use personal protective equipment as required.

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6.2. Environmental precautions

Do not flush into surface water or sanitary sewer system.

6.3. Methods and material for containment and cleaning up

Soak up with inert absorbent material. Keep in suitable, closed containers for disposal.

6.4. Reference to other sections

Refer to protective measures listed in Sections 8 and 13.

SECTION 7: HANDLING AND STORAGE

7.1. Precautions for safe handling

Wear personal protective equipment/face protection. Ensure adequate ventilation. Avoid contact with skin, eyes or clothing. Avoid
ingestion and inhalation. Wash hands before breaks and immediately after handling the product.

Hygiene Measures
Handle in accordance with good industrial hygiene and safety practice. Keep away from food, drink and animal feeding stuffs. Do
not eat, drink or smoke when using this product. Remove and wash contaminated clothing and gloves, including the inside, before
re-use. Wash hands before breaks and after work.

7.2. Conditions for safe storage, including any incompatibilities

Keep containers tightly closed in a dry, cool and well-ventilated place.

Technical Rules for Hazardous Substances (TRGS) 510 Class 12

Storage Class (LGK) (Germany)

7.3. Specific end use(s)

Use in laboratories

SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION

8.1. Control parameters

Exposure limits
List source(s):

Component The United Kingdom European Union Ireland

Copper (II) sulfate pentahydrate (1:1:5) STEL: 2 mg/m3 15 min
TWA: 1 mg/m3 8 hr

Biological limit values

This product, as supplied, does not contain any hazardous materials with biological limits established by the region specific
regulatory bodies

Derived No Effect Level (DNEL) / Derived Minimum Effect Level (DMEL)

No information available
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Predicted No Effect Concentration (PNEC)
No information available.

8.2. Exposure controls

Engineering Measures
Ensure that eyewash stations and safety showers are close to the workstation location. Ensure adequate ventilation, especially in
confined areas.
Wherever possible, engineering control measures such as the isolation or enclosure of the process, the introduction of process or
equipment changes to minimise release or contact, and the use of properly designed ventilation systems, should be adopted to
control hazardous materials at source

Personal protective equipment

Eye Protection Goggles (European standard - EN 166)

Hand Protection Protective gloves

Glove material Breakthrough time Glove thickness EU standard Glove comments

Natural rubber See manufacturers - EN 374 (minimum requirement)
Nitrile rubber recommendations
Neoprene
PVC
Skin and body protection Wear appropriate protective gloves and clothing to prevent skin exposure.

Inspect gloves before use.

Please observe the instructions regarding permeability and breakthrough time which are provided by the supplier of the gloves.
(Refer to manufacturer/supplier for information)
Ensure gloves are suitable for the task: Chemical compatability, Dexterity, Operational conditions, User susceptibility, e.g.
sensitisation effects, also take into consideration the specific local conditions under which the product is used, such as the danger
of cuts, abrasion.
Remove gloves with care avoiding skin contamination.

Respiratory Protection No protective equipment is needed under normal use conditions.

Large scale/emergency use Use a NIOSH/MSHA or European Standard EN 136 approved respirator if exposure limits
are exceeded or if irritation or other symptoms are experienced

Small scale/Laboratory use Maintain adequate ventilation

Environmental exposure controls Prevent product from entering drains. Do not allow material to contaminate ground water
system. Local authorities should be advised if significant spillages cannot be contained.

SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES

9.1. Information on basic physical and chemical properties

Physical State Liquid

Appearance Clear, Blue

Odor Odorless
Odor Threshold No data available
Melting Point/Range No data available
Softening Point No data available
Boiling Point/Range No information available
Flammability (liquid) No data available
Flammability (solid,gas) Not applicable Liquid
Explosion Limits No data available
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Flash Point No information available Method - No information available
Autoignition Temperature No data available
Decomposition Temperature No data available
pH No information available
Viscosity No data available
Water Solubility Soluble
Solubility in other solvents No information available
Partition Coefficient (n-octanol/water)
Vapor Pressure No data available
Density / Specific Gravity 1.21
Bulk Density Not applicable Liquid
Vapor Density No data available (Air = 1.0)
Particle characteristics Not applicable (liquid)

9.2. Other information

SECTION 10: STABILITY AND REACTIVITY

10.1. Reactivity
None known, based on information available

10.2. Chemical stability

Stable under normal conditions.

10.3. Possibility of hazardous reactions

Hazardous Polymerization Hazardous polymerization does not occur.

Hazardous Reactions None under normal processing.

10.4. Conditions to avoid

Excess heat.

10.5. Incompatible materials

No information available.

10.6. Hazardous decomposition products

Thermal decomposition can lead to release of irritating gases and vapors.

SECTION 11: TOXICOLOGICAL INFORMATION

11.1. Information on hazard classes as defined in Regulation (EC) No 1272/2008

Product Information No acute toxicity information is available for this product

(a) acute toxicity;

Oral Based on ATE data, the classification criteria are not met
ATE = 30,062 mg/kg
Dermal Based on available data, the classification criteria are not met
Inhalation Based on available data, the classification criteria are not met

Toxicology data for the components

Component LD50 Oral LD50 Dermal LC50 Inhalation

Sodium citrate 5400 mg/kg (Mouse) - -
Sodium carbonate 2800 mg/kg ( Rat ) > 2000 mg/kg (rabbit) 2.3 mg/l 2h (Rat)
Copper (II) sulfate pentahydrate (1:1:5) LD50 = 960 mg/kg ( Rat ) LD50 > 8 g/kg ( Rabbit ) -

Sodium bicarbonate LD50 = 4220 mg/kg ( Rat ) - -

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Water - - -

Component ECHA (RAC) ATE (Oral) ECHA (RAC) ATE (Dermal) ECHA (RAC) ATE (Inhalation)
Copper (II) sulfate pentahydrate (1:1:5) ATE = 481 mg/kg bw - -

(b) skin corrosion/irritation; No data available

(c) serious eye damage/irritation; Category 2

(d) respiratory or skin sensitization;

Respiratory No data available
Skin No data available

(e) germ cell mutagenicity; No data available

(f) carcinogenicity; No data available

There are no known carcinogenic chemicals in this product

(g) reproductive toxicity; No data available

(h) STOT-single exposure; No data available

(i) STOT-repeated exposure; No data available

Target Organs No information available.

(j) aspiration hazard; No data available

Symptoms / effects,both acute and No information available.

delayed

11.2. Information on other hazards

Endocrine Disrupting Properties Assess endocrine disrupting properties for human health. This product does not contain any
known or suspected endocrine disruptors.

SECTION 12: ECOLOGICAL INFORMATION

12.1. Toxicity
Ecotoxicity effects Contains a substance which is:. The product contains following substances which are
hazardous for the environment. Very toxic to aquatic organisms.

Component Freshwater Fish Water Flea Freshwater Algae

Sodium citrate LC50: 18000 - 32000 mg/L, 96h EC50: 5600 - 10000 mg/L, 48h
(Poecilia reticulata) (Daphnia magna)

Sodium carbonate Lepomis macrochirus: LC50: 300 EC50: = 265 mg/L, 48h
mg/L/96h (Daphnia magna)
Gambusia affinis: LC50: 740
mg/L/96h
Copper (II) sulfate pentahydrate (1:1:5) Onchorhynchus mykiss: LC50 = EC50 = 0.24 mg/L/48h
0.1-2.5 mg/L/96h
Sodium bicarbonate LC50: 8250 - 9000 mg/L, 96h EC50: 2350 mg/L/48h EC50: 650 mg/L/120h
static (Lepomis macrochirus)
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Component Microtox M-Factor

Sodium citrate EC50 1800 - 3200 mg/L 8 h
Sodium carbonate -
Copper (II) sulfate pentahydrate (1:1:5) Photobacterium phosphoreum: EC50 = 0.25 10 (Acute)
mg/L/30min as Cu++ 1 (Chronic)
Photobacterium phosphoreum EC50= 1.3 mg/L/5
min as Cu++
Sodium bicarbonate -

12.2. Persistence and degradability

Persistence Soluble in water, Persistence is unlikely, based on information available.
Degradation in sewage Contains substances known to be hazardous to the environment or not degradable in waste
treatment plant water treatment plants.

12.3. Bioaccumulative potential Bioaccumulation is unlikely

12.4. Mobility in soil The product is water soluble, and may spread in water systems . Will likely be mobile in the
environment due to its water solubility. Highly mobile in soils

12.5. Results of PBT and vPvB No data available for assessment.

assessment

12.6. Endocrine disrupting

properties
Endocrine Disruptor Information This product does not contain any known or suspected endocrine disruptors

12.7. Other adverse effects

Persistent Organic Pollutant This product does not contain any known or suspected substance
Ozone Depletion Potential This product does not contain any known or suspected substance

SECTION 13: DISPOSAL CONSIDERATIONS

13.1. Waste treatment methods

Waste from Residues/Unused Waste is classified as hazardous. Dispose of in accordance with the European Directives
Products on waste and hazardous waste. Dispose of in accordance with local regulations.

Contaminated Packaging Dispose of this container to hazardous or special waste collection point.

European Waste Catalogue (EWC) According to the European Waste Catalog, Waste Codes are not product specific, but
application specific.

Other Information Do not flush to sewer. Waste codes should be assigned by the user based on the
application for which the product was used. Do not empty into drains. Do not let this
chemical enter the environment.

SECTION 14: TRANSPORT INFORMATION

IMDG/IMO Not regulated

14.1. UN number
14.2. UN proper shipping name
14.3. Transport hazard class(es)
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14.4. Packing group

ADR Not regulated

14.1. UN number
14.2. UN proper shipping name
14.3. Transport hazard class(es)
14.4. Packing group

IATA Not regulated

14.1. UN number
14.2. UN proper shipping name
14.3. Transport hazard class(es)
14.4. Packing group

14.5. Environmental hazards No hazards identified

14.6. Special precautions for user No special precautions required.

14.7. Maritime transport in bulk Not applicable, packaged goods

according to IMO instruments

SECTION 15: REGULATORY INFORMATION

15.1. Safety, health and environmental regulations/legislation specific for the substance or mixture

International Inventories
Europe (EINECS/ELINCS/NLP), China (IECSC), Taiwan (TCSI), Korea (KECL), Japan (ENCS), Japan (ISHL), Canada (DSL/NDSL), Australia
(AICS), New Zealand (NZIoC), Philippines (PICCS). US EPA (TSCA) - Toxic Substances Control Act, (40 CFR Part 710)

Component CAS No EINECS ELINCS NLP IECSC TCSI KECL ENCS ISHL
Sodium citrate 68-04-2 200-675-3 - - X X KE-20843 X X
Sodium carbonate 497-19-8 207-838-8 - - X X KE-31380 X X
Copper (II) sulfate pentahydrate 7758-99-8 - - - X X - X -
(1:1:5)
Sodium bicarbonate 144-55-8 205-633-8 - - X X KE-31360 X X
Water 7732-18-5 231-791-2 - - X X KE-35400 X -

Component CAS No TSCA TSCA Inventory DSL NDSL AICS NZIoC PICCS
notification -
Active-Inactive
Sodium citrate 68-04-2 X ACTIVE X - X X X
Sodium carbonate 497-19-8 X ACTIVE X - X X X
Copper (II) sulfate pentahydrate 7758-99-8 - - - - X X X
(1:1:5)
Sodium bicarbonate 144-55-8 X ACTIVE X - X X X
Water 7732-18-5 X ACTIVE X - X X X

Legend: X - Listed '-' - Not Listed KECL - NIER number or KE number (http://ncis.nier.go.kr/en/main.do)

Authorisation/Restrictions according to EU REACH

Component CAS No REACH (1907/2006) - REACH (1907/2006) - REACH Regulation (EC

Annex XIV - Substances Annex XVII - Restrictions 1907/2006) article 59 -
Subject to Authorization on Certain Dangerous Candidate List of
Substances Substances of Very High
Concern (SVHC)
Sodium citrate 68-04-2 - - -
Sodium carbonate 497-19-8 - Use restricted. See item -
75.
(see link for restriction
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details)
Copper (II) sulfate pentahydrate 7758-99-8 - Use restricted. See item -
(1:1:5) 75.
(see link for restriction
details)
Sodium bicarbonate 144-55-8 - - -
Water 7732-18-5 - - -

REACH links
https://echa.europa.eu/substances-restricted-under-reach

Seveso III Directive (2012/18/EC)

Component CAS No Seveso III Directive (2012/18/EC) - Seveso III Directive (2012/18/EC) -
Qualifying Quantities for Major Accident Qualifying Quantities for Safety Report
Notification Requirements
Sodium citrate 68-04-2 Not applicable Not applicable
Sodium carbonate 497-19-8 Not applicable Not applicable
Copper (II) sulfate 7758-99-8 Not applicable Not applicable
pentahydrate (1:1:5)
Sodium bicarbonate 144-55-8 Not applicable Not applicable
Water 7732-18-5 Not applicable Not applicable

Regulation (EC) No 649/2012 of the European Parliament and of the Council of 4 July 2012 concerning the export and
import of dangerous chemicals
Not applicable

Contains component(s) that meet a 'definition' of per & poly fluoroalkyl substance (PFAS)?
Not applicable

Take note of Directive 98/24/EC on the protection of the health and safety of workers from the risks related to chemical agents at
work .

National Regulations

UK - Take note of Control of Substances Hazardous to Health Regulations (COSHH) 2002 and 2005 Amendment

WGK Classification Water endangering class = 1 (self classification)

Component Germany - Water Classification (AwSV) Germany - TA-Luft Class

Sodium citrate WGK1
Sodium carbonate WGK1
Sodium bicarbonate WGK1

15.2. Chemical safety assessment

Chemical Safety Assessment/Reports (CSA/CSR) are not required for mixtures

SECTION 16: OTHER INFORMATION

Full text of H-Statements referred to under sections 2 and 3

H302 - Harmful if swallowed
H315 - Causes skin irritation
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H319 - Causes serious eye irritation
H400 - Very toxic to aquatic life
H410 - Very toxic to aquatic life with long lasting effects

Legend

CAS - Chemical Abstracts Service TSCA - United States Toxic Substances Control Act Section 8(b)
Inventory
EINECS/ELINCS - European Inventory of Existing Commercial Chemical DSL/NDSL - Canadian Domestic Substances List/Non-Domestic
Substances/EU List of Notified Chemical Substances Substances List
PICCS - Philippines Inventory of Chemicals and Chemical Substances ENCS - Japanese Existing and New Chemical Substances
IECSC - Chinese Inventory of Existing Chemical Substances AICS - Australian Inventory of Chemical Substances
KECL - Korean Existing and Evaluated Chemical Substances NZIoC - New Zealand Inventory of Chemicals

WEL - Workplace Exposure Limit TWA - Time Weighted Average

ACGIH - American Conference of Governmental Industrial Hygienists IARC - International Agency for Research on Cancer
DNEL - Derived No Effect Level Predicted No Effect Concentration (PNEC)
RPE - Respiratory Protective Equipment LD50 - Lethal Dose 50%
LC50 - Lethal Concentration 50% EC50 - Effective Concentration 50%
NOEC - No Observed Effect Concentration POW - Partition coefficient Octanol:Water
PBT - Persistent, Bioaccumulative, Toxic vPvB - very Persistent, very Bioaccumulative

ADR - European Agreement Concerning the International Carriage of ICAO/IATA - International Civil Aviation Organization/International Air
Dangerous Goods by Road Transport Association
IMO/IMDG - International Maritime Organization/International Maritime MARPOL - International Convention for the Prevention of Pollution from
Dangerous Goods Code Ships
OECD - Organisation for Economic Co-operation and Development ATE - Acute Toxicity Estimate
BCF - Bioconcentration factor VOC - (Volatile Organic Compound)
Key literature references and sources for data
https://echa.europa.eu/information-on-chemicals
Suppliers safety data sheet, Chemadvisor - LOLI, Merck index, RTECS

Classification and procedure used to derive the classification for mixtures according to Regulation (EC) 1272/2008 [CLP]:
Physical hazards On basis of test data
Health Hazards Calculation method
Environmental hazards Calculation method

Training Advice
Chemical hazard awareness training, incorporating labelling, Safety Data Sheets (SDS), Personal Protective Equipment (PPE) and
hygiene.
Use of personal protective equipment, covering appropriate selection, compatibility, breakthrough thresholds, care, maintenance, fit
and standards.
First aid for chemical exposure, including the use of eye wash and safety showers.

Creation Date 05-May-2010

Revision Date 09-Feb-2024
Revision Summary Not applicable.

This safety data sheet complies with Regulation UK SI 2019/758 and UK SI 2020/1577 as
amended. .
.
Disclaimer
The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the
date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage,
transportation, disposal and release and is not to be considered a warranty or quality specification. The information
relates only to the specific material designated and may not be valid for such material used in combination with any other
materials or in any process, unless specified in the text

End of Safety Data Sheet