About PermaSure+ and the world’s first
Ph. Eur. 5.24 compliant FT-IR imaging
Dr. Oliver Maute, Validation Manager, Bruker Optics GmbH & Co. KG, Ettlingen, Germany
Dr. Simon Hugo Schlindwein, Validation Support, Bruker Optics GmbH & Co. KG, Ettlingen, Germany
What is chemical imaging?
Chemical imaging is an exceptionally effective tool to perform a very detailed and spatially
resolved chemical analysis based on a sample’s spectroscopic properties.
If you’re unfamiliar with chemical imaging please take a look at our short introduction to
FT-IR imaging.
What is the intention of Ph. Eur. 5.24?
„It offers specific recommendations to assess the
performance of chemical imaging systems for the
qualitative and quantitative exploitation of images.“
(Ph. Eur. Chapter 5.24; 1. Scope)
The value of chemical imaging is tremendous.
For manufacturing, however, its use was limited
due to missing guidelines. In 2016, the European
Pharmacopoeia introduced the chapter 5.24 to
address chemical imaging in its many forms, such
as infrared, hyperspectral and raman imaging. without PermaSure+
Why was compliance to Ph. Eur. 5.24 so hard
to achieve? Figure 1: Variance of the
position of the waterva-
„Optimal wavenumber accuracy is critical and should be achieved at the same level as for a por band at 1576.13 cm -1
standard spectrometer.“ when measured without
(Ph. Eur. 5.24; 3-2-2-1. Spectral Axis) PermaSure+ calibration.
This may sound trivial but is quite hard to do. In an FT-IR microscope the IR rays that reach
different pixels on the array detector take slightly different ways within the interferometer.
This must be considered when calculating the scale of the wavenumber axis. As a result,
every pixel of the chemical image needs to have an individual calibration of the waven-
umber axis. Figure 1 shows the wavenumber accuracy for an FT-IR microscope without
Bruker’s PermaSure+ calibration. The wavenumber accuracy strongly differs from pixel to
pixel.
How did Bruker realize compliance to Ph.
Eur. 5.24?
„Spectral axis calibration assigns wavelength and
intensity values to every mapped pixel of the sample
surface.“
(Ph. Eur. 5.24; 3-2-2-1. Spectral Axis)
PermaSure+ introduces a revolutionary pixel-
by-pixel laser wavenumber calibration that takes with PermaSure+
the aforementioned problems into account.
without PermaSure+
This brings the achievable x-axis accuracy to an
unprecedented level on par with single-element
detectors or, to be exact, “the same level” as
standard spectrometers. Figure 2 elucidates the
effect of PermaSure+ on the wavenumber accuracy of an FPA imaging detector. Through
this calibration, each pixel now has a better and above all comparable wavenumber Figure 2: The same
accuracy. detector pixels as Figure
1 but with PermaSure+
enabled.
Better instrument - better results.
With the introduction of the LUMOS II Bruker is trying to make FT-IR imaging available
to all applications and industries. By introducing new and exciting technologies, it is our
goal to provide customers with the performance and usability they expect from a state-of-
the-art scientific instrument. If you have further questions please contact your local sales
representative or use our webform to submit your request.
Bruker Optics is continually improving its products and reserves the right to change specifications without notice.
Bruker’s newest addition INVENIO is Bruker’s
to its FT-IR microscopy platform solution to
line-up: the LUMOS II. FT-IR. It offers research
Usability, utility and grade multispectral
© 2022 Bruker Optics BOPT-01
performance are its performance from FIR
main focus, providing to VIS/UV. Coupled to
>900 spectra per the HYPERION II FT-IR
second, PermaSure+, full imaging microscope
automation and 100% it gives access to FPA
reliability. Watch our imaging technology.
product movie here.
Bruker Optics GmbH & Co. KG Bruker Optics
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