Lista De Primers – EPPO QBANK

Primer Directio
Locus Sequence (5'-3') Reference
name n

ITS-4 Reverse TCC TCC GCT TAT TGA TAT GC White et al. (1990)

GGA AGT AAA AGT CGT AAC

ITS-5 Forward White et al. (1990)
AAG G

5.8S nrRNA gene with the CTT GGT CAT TTA GAG GAA
ITS1F Forward Gardes & Bruns (1993)
two flanking internal GTA A
transcribed spacers (ITS) Vilgalys & Hester
LR5 Reverse TCC TGA GGG AAA CTT CG
(1990)

de Hoog and Gerrits

V9G Forward TTA CGT CCC TGC CCT TTG TA
van den Ende (1998)

CTG AAC GCC TCT AAG TCA Vilgalys lab, Duke

Intergenic Spacer 1 of LR12R Forward
GAA University
nRNA operon (IGS1)
Seq5S Reverse CAG ATC AGA CGG GAT GCG GT Woodhall et al. (2007)

Partial 18S ribosomal NS1 Forward GTA GTC ATA TGC TTG TCT C White et al. (1990)
RNA gene (SSU) NS4 Reverse CTT CCG TCA ATT CCT TTA AG White et al. (1990)

Rehner & Samuels

LR0R Forward GTA CCC GCT GAA CTT AAG C
(1994)

Vilgalys & Hester

Partial 28S ribosomal LR5 Reverse TCC TGA GGG AAA CTT CG
(1990)
RNA gene (LSU)
Vilgalys & Hester
LR7 Reverse TAC TAC CAC CAA GAT CT
(1990)

LSU1Fd Forward GRA TCA GGT AGG RAT ACC CG Crous et al. (2009)

ACT-512F Forward ATG TGC AAG GCC GGT TTC GC Carbone & Kohn (1999)

ACT-783R Reverse TAC GAG TCC TTC TGG CCC AT Carbone & Kohn (1999)
Partial actin gene (ACT) Groenewald et al.
ACT2Rd Reverse ARR TCR CGD CCR GCC ATG TC (2013) & Quaedvlieg et
al. (2011)

Apn1W1F Forward ATG GAG CAC AAA AAC GAA CA Crouch et al. (2009)
Partial apurinic DNA lyase
GCG GAG CAG AGG ATG TAG
gene (APN2) Apn1W1R Reverse Crouch et al. (2009)
TC

Partial beta-tubulin gene ACC CTC AGT GTA GTG ACC Glass & Donaldson
Bt-2b Reverse
(Tub2) CTT GGC (1995)

GCC AAG TTC TGG GAG GTC

Btub_F1 Forward Blair et al. (2008)
ATC

AAC ATG CGT GAG ATT GTA O’Donnell & Cigelnik

T1 Forward
AGT (1997)

T2 Reverse TAG TGA CCC TTG GCC CAG O’Donnell & Cigelnik
TTG (1997)
 Lista De Primers – EPPO QBANK

Primer Directio
Locus Sequence (5'-3') Reference
name n

Groenewald et al.
TUB2Fd GTB CAC CTY CAR ACC GGY
Forward (2013) & Woudenberg
(=BT2Fd) CAR TG
et al. (2009)

Groenewald et al.
TUB4Rd CCR GAY TGR CCR AAR ACR
Reverse (2013) & Woudenberg
(=BT4R) AAG TTG TC
et al. (2009)

TUBUR1
CCT GGT ACT GCT GGT ACT
(= Reverse Kroon et al. (2004)
CAG
BTubR1)

GAG TTC AAG GAG GCC TTC

CAL-228F Forward Carbone & Kohn (1999)
TCC C

TTC AAG GAG GCC TTC TCC

CAL-235F Forward Quaedvlieg et al. (2012)
CTC TT

CAL-737R Reverse CAT CTT TCT GGC CAT CAT GG Carbone & Kohn (1999)

Groenewald et al.
Partial calmodulin gene TGR TCN GCC TCD CGG ATC
CAL2Rd Reverse (2013) & Quaedvlieg et
(CAL) ATC TC
al. (2011)

CL1 Forward GAR TWC AAG GAG GCC TTC TC O’Donnell et al. (2000)

CL1C Forward GAA TTC AAG GAG GCC TTC TC Weir et al. (2012)

CL2A Reverse TTT TTG CAT CAT GAG TTG GAC O’Donnell et al. (2000)

CTT CTG CAT CAT GAG CTG

CL2C Reverse Weir et al. (2012)
GAC

CHS- TGG AAG AAC CAT CTG TGA

Reverse Carbone & Kohn (1999)
Partial chitin synthase 1 354R GAG TTG
gene (CHS-1) TGG GGC AAG GAT GCT TGG
CHS-79F Forward Carbone & Kohn (1999)
AAG AAG

Cox1MlpA TAA GAT GAC TTT ATA GTA CCA

Forward Feau et al. (2009)
F A

Cox1MlpA
Reverse GCT CCT ACC ATT ACM G Feau et al. (2009)
R

OomCoxI- CYT CHG GRT GWC CRA AAA

Reverse Robideau et al. (2011)
Partial cytochrome Levlo ACC AAA

oxidase 1 (CO1) OomCoxI- TCA WCW MGA TGG CTT TTT

Forward Robideau et al. (2011)
Levup TCA AC

TCA GGR TTA YTA GGW ACA Nguyen & Seifert

PezizF Forward
GCA TTT (2008)

ACC TCA GGR TGY CCG AAG Nguyen & Seifert

PezizR Reverse
AAT (2008)
 Lista De Primers – EPPO QBANK

Primer Directio
Locus Sequence (5'-3') Reference
name n

CCT TGG CAA TTA GGA TTT CAA

Partial cytochrome FM75 Forward Martin & Tooley (2003)
GAT
oxidase 2 (COX2)
FM78 Reverse ACA AAT TTC ACT ACA TTG TCC Martin & Tooley (2003)

Stephenson et al.
GSF1 Forward ATG GCC GAG TAC ATC TGG
(1997)

Partial glutamine GSF3 Forward GCC GGT GGA GGA ACC GTC G Weir et al. (2012)

synthetase gene (GS) Stephenson et al.

GSR1 Reverse GAA CCG TCG AAG TTC CAG
(1997)

GSR2 Reverse GAA CCG TCG AAG TTC CAC Weir et al. (2012)

GCC GTC AAC GAC CCC TTC

GDF Forward Templeton et al. (1992)
ATT GA

GCC GTC AAC GAC CCC TTC

GDF1 Forward Guerber et al. (2003)
ATT GA

GGG TGG AGT CGT ACT TGA

GDR Reverse Templeton et al. (1992)
GCA TGT

GGG TGG AGT CGT ACT TGA

Partial glyceraldehyde-3- GDR1 Reverse Guerber et al. (2003)
GCA TGT
phosphate
dehydrogenase gene
(GAPDH) ATT GGC CGC ATC GTC TTC
Gpd1-LM Forward Myllys et al. (2002)
CGC AA

Gpd2-LM Reverse CCC ACT CGT TGT CGT ACC A Myllys et al. (2002)

CTC RGM RGC RGC CTT GAT

GPDHR2 Reverse Glienke et al. (2011)
GG

Partial histone H3 gene CYLH3F Forward AGG TCC ACT GGT GGC AAG Crous et al. (2004)
(HIS3) CYLH3R Reverse AGC TGG ATG TCC TTG GAC TG Crous et al. (2004)

Partial manganese ATG GCA GCC TTT CCG TTG

SOD507F Forward Crouch et al. (2006)
superoxide dismutase AGA TAC
gene (SOD2) AGT TGA CAT GAA GCC ACC
SOD507R Reverse Crouch et al. (2006)
TAC AGC

GCC CAC AGT ACA TAT TGC

SOD625F Forward Crouch et al. (2006)
CTA AGC

TCA TCC CGG GAG CCA GAA

SOD625R Reverse Crouch et al. (2006)
AAC CT

SODglo2- Forward CAG ATC ATG GAG CTG CAC CA Moriwaki & Tsukiboshi
F (2009)
 Lista De Primers – EPPO QBANK

Primer Directio
Locus Sequence (5'-3') Reference
name n

SODglo2- Moriwaki & Tsukiboshi

Reverse TAG TAC GCG TGC TCG GAC AT
R (2009)

ACG GCA AAC GGC TCA GGG

Mat1M72F Forward Crouch et al. (2009)
Partial mating type locus AGT G
gene (MAT1-2) Mat1M72 AAT GCC GAG TCC CAC GAG
Reverse Crouch et al. (2009)
R GTT CG

Partial Ras-related protein Ypt1F Forward CGA CCA TYG GYG TKG ACT TT Chen & Roxby (1996)
gene (Ypt1) Ypt5R Reverse GCA GCT TGT TSA CGT TCT CR Schena & Cooke (2006)

RPB1-Af Stiller & Hall (1997),

(=RPB1- Forward GAR TGY CCD GGD CAY TTY GG modification by Giho
Partial RNA polymerase II Ac) Sung
largest subunit (RPB1) Matheny et al (2002),
CCN GCD ATN TCR TTR TCC
RPB1-Cr Reverse modification by Giho
ATR TA
Sung

fRPB2- ACM ANN CCC CAR TGN GWR

Reverse Quaedvlieg et al. (2011)
Partial RNA polymerase II 414R TTR TG

second largest subunit GAY GAY MGW GAT CAY TTY

fRPB2-5F Forward Liu et al. (1999)
(RPB2) GG

fRPB2-7R Reverse CCC ATW GCY TGC TTM CCC AT Liu et al. (1999)

Partial translation GGA RGT ACC AGT SAT CAT

EF-2 Reverse O'Donnell et al. (1998)
elongation factor 1-alpha GTT
gene (TEF) EF1-728F Forward CAT CGA GAA GTT CGA GAA GG Carbone & Kohn (1999)

EF1-986R Reverse TAC TTG AAG GAA CCC TTA CC Carbone & Kohn (1999)

EFCF1 Forward AGT GCG GTG GTA TCG ACA AG Jones et al. (2011)

EFCF2 Reverse TGC TCA CGG GTC TGG CCA T Oliveira et al. (2015)

EFCF6 Reverse CAT GTC ACG GAC GGC GAA AC Jones et al. (2011)

ELONGF1
Forward TCA CGA TCG ACA TTG CCC TG Kroon et al. (2004)
(=EF1AF)

ELONGR1
Reverse ACG GCT CGA GGA TGA CCA TG Kroon et al. (2004)
(=EF1AR)

TEF1-
983F GCY CCY GGH CAY CGT GAY The Hibbett lab primer
Forward
(=EF1- TTY AT protocols
983F)

TEF1- Reverse AT GAC ACC RAC RGC RAC RGT The Hibbett lab primer
2218R YTG protocols
(=EF1-
 Lista De Primers – EPPO QBANK

Primer Directio
Locus Sequence (5'-3') Reference
name n

2218R)