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Athens - Microbe - Twin Tiers

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Twin Tiers Invitational

Athens, PA

MICROBE MISSION TEST


Use the following information to answer #1-5.

A student was observing a microbial sample in a Petri dish. This is what it looks like under a
microscope (Fig. 1).

Figure 1.

The student also created a table showing the growth of the microbe over time.

Time Number of microbes


0 minutes 30
5 minutes 150
10 minutes 750
15 minutes 3750

1. The microscope above shows a sample of what?


a. diatoms
b. yeast
c. lichens
d. algae
e. ciliates

2. If the student constructed a graph of the information in the table, what would be the
manipulated variable?
a. sample used
b. time
c. number of microbes
d. concentration
e. size

3. Is the data in the table an example of an arithmetic sequence or geometric sequence?


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4. If the trend continues, how many microbes will there be in the Petri dish in 25 minutes?

5. What type of microbe is the sample in the microscope? ___________

*Use figure 2 to answer questions #6-10.

Figure 2.

6. What type of microbe growth is shown above?


a. continuous culture c. semi-continuous culture
b. open culture d. closed culture

7. What is “A” called in the microbial growth shown in figure 2?

8. What is “B” called in the microbial growth shown in figure 2?

9. What is “C” called in the microbial growth shown in figure 2?

10. What is “D” called in the microbial growth shown in figure 2?

Matching: Match the disease with the type of pathogen that produces it.

A. Bacterial B.Viral C. Fungal D. Protozoan E. Prion

11. Mumps 18. Strep Throat


12. Botulism 19. Ebola
13. Tetanus 20. Ebola
14. Thrush 21. Peptic Ulcer
15. Yellow Fever 22. Mad Cow Disease
16. Malaria 23. Ringworm
17. Dental Caries 24. Chlamydiasis

*Use figure 3 to answer questions #25-28.

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25. What kind of microscope is this?

26. Identify the letter that represents the diaphragm.

27. Identify which letter represents the fine adjustment.

28. Which letter(s) is/are involved in determining total magnification?

29. What are the 2 cycles a virus could follow in a host?

30. Choose which of the following are plausible symptoms of legionellosis. You may circle
more than one answer.
a. dry cough
b. intestinal disorders
c. anemia
d. vomiting

31. When centrioles are destroyed by hypothetical microbes in an animal cell, what function is
essentially terminated?
a. ATP production

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b. metabolism of sugars
c. endocytosis
d. mitosis

32. Which of the following statements BEST describes the Endosymbiotic Theory?
a. prokaryotic cells evolved from specialized eukaryotes living inside one another.
b. viruses evolved from protein-infectious particles.
c. eukaryotic cells evolved from specialized prokaryotes living inside one another.
d. eukaryotic cells evolved when infoldings of the plasma membrane engulfed bacteria.

33. A group of spherical bacteria which occurs in a chain is called


a. sarcina
b. streptococci
c. staphylococci
d. diplococci

34. Lichens are a symbiotic association between a photosynthetic organism and a


a. fungus
b. virus
c. protozoan
d. bacterium

* Put these in order from largest to smallest:

35. ___ e. red blood cell


36. ___ f. amoeba
37. ___ g. E. coli
38. ___ h. carbon atom
39. ___ i. rhinovirus
40. ___ j. paramecium
41. ___ k. yeast
42. ___ l. phage

43. Genetic variation among bacterial populations may result from all of the following
processes except
a. transduction
b. mutation
c. meiosis
d. conjugation

44. You discover something green and fuzzy on some food that has been in the refrigerator
for a long time. This organism is most likely a(n)
a. Archeae
b. bacterium
c. alga
d. fungus

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45. A scientist discovers some cells underneath the surface of Antarctica. This organism is
most likely a(n)
a. Archeae
b. virus
c. alga
d. fungus

46. Which of the following groups of organisms represent the main decomposers in the soil?
a. bacteria and viruses c. fungi and viruses
b. bacteria and fungi d. algae and fungi

47. NASA scientist have recently reported that they have isolated an organism from a lake in
California. The lake has a 10% salt concentration (the oceans are about 3% salt) and a pH of
10. What is the most likely type of organism isolated?

a. Fungus b. Archeae c. Eubacteria d. Plant like protist(alga)

*Match the human body system that is typically affected by the following diseases.
Some answers may be used more than once; others not all. Each disease will have
only 1 answer.

Disease Body System

48. Polio a. Circulatory

49. MRSA b. Digestive

50.Tuberculosis c. Respiratory

51. Pertussis d. Integumentary

52. Cholera e. Nervous

53. Which of the following diseases are the result of arthropod bites?

a. Botulism and mononucleosis b. Anthrax and tetanus


c.West Nile Fever and Rocky Mountain Spotted fever d. Herpes and Legionaire’s Disease

54. What structure is responsible for the properties of Eubacteria?

a. spore b. cell wall c. flagellum d. answer not given


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55. You have found a single cell that is mobile, and when you examine it in the microscope
you discover a small and a large nucleus. What is the most likely type of microbe that you
have found?

a. virus b. fungus c. protozoan d. bacterium

56. Which group of microbes does not have a cell wall?

a. Fungi b. Archae c. Eubacteria d. Animal-like protists

57. All of the following statements about the causative agent of AIDS are true except

a. It contains RNA instead of DNA

b. It infects cells of the immune system

c. It produces double stranded nucleic acids that replicate in the cytoplasm

d. It mutates while in the body of a victim.

* (#58-63) Using letters, indicate which type of microscope is MOST suitable for the
situation.

58. Determining the shape of bacterial cells


59. Observing various microbes in pond water a. Dissecting
60. Examining a tissue for viruses b. Compound Light
61. Examining mobile protozoans c. Transmission Electron
62. Examining the surface of pollen grains d. Scanning Electron
63. Examining cells for the detailed structure of mitochondria

64. Because penicillin prevents peptidoglycan synthesis, it is more effective on


_______________ cells.
a. Gram positive bacteria b. Gram negative bacteria

65. When flagella are located around the entire bacterial cell, the arrangement is called
a. polar b. random c. bipolar d. peritrichous

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School: Score:
Team #:

MICROBE MISSION ANSWER SHEET

1. 34.
2. 35.
3. 36.
4. 37.
5. 38.
6. 39.
7. 40.
8. 41.
9. 42.
10. 43.
11. 44.
12. 45.
13. 46.
14. 47.
15. 48.
16. 49.
17. 50.
18. 51.
19. 52.
20. 53.
21. 54.
22. 55.
23. 56.
24. 57.
25. 58.
26. 59.
27. 60.
28. 61.
29. 62.
30. 63.
31. 64.
32. 65.
33.

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IMAGES

66.

67.

68.

69.

70.

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IMAGES

66. Discuss what is going on in this picture. What causes this to happen? What are the
ecological impacts?

67. Name the lab technique used in this picture and discuss its purpose.

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68. Which letter depicts Gram negative bacteria?

69. State the morphology and arrangement.

70. State the name of this microbe. Describe its defining features.

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School: Score: /83
Team #:

MICROBE MISSION ANSWER (KEY)

1. B 34. A
2. B 35. F
3. Geometric Sequence 36. J
4. 93,750 37. E
5. Fungi 38. K
6. Closed Culture 39. G
7. Lag Phase 40. L
8. Log (exponential) Phase 41. I
9. Stationary Phase 42. H
10. Death (decline) phase 43. C
11. B 44. D
12. A 45. A
13. A 46. B
14. C 47. B
15. B 48. E
16. D 49. D
17. A 50. C
18. A 51. C
19. B 52. B
20. B 53. C
21. A 54. B
22. E 55. C
23. C 56. D
24. H 57. C
25. Compound Light Microscope 58. B
26. E 59. B
27. K 60. D
28. G & C –must have both (2pts) 61. B
29. Lytic & Lysogenic – must have both (2pts) 62. D
30. A, B, D – must have all (3pts) 63. C
31. D 64. A
32. C 65. D
33. B

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IMAGES

(5 pts) 66. This picture is showing an Algal Bloom. An algal bloom is a result of an
abundance of nutrients, especially phosphorus. Because of the excess nutrients the algae
increase their population dramatically. Each alga is short-lived, and the result is a high
concentration of dead organic matter which starts to decay. The decay process consumes
dissolved oxygen in the water, resulting in hypoxic conditions. Without sufficient dissolved
oxygen in the water, animals and plants may die off in large numbers.

(3 pts) 67. This is an example of the Streak Technique. In microbiology, Streaking is a


technique used to isolate a pure strain from a single species of microorganism, often
bacteria. Samples can then be taken from the resulting colonies and a microbiological culture
can be grown on a new plate so that the organism can be identified, studied, or tested.

68. B

69. Staphylococcus

(4 pts) 70. Euglena. Euglena is a unicellular flagellate protist. They are autotrophic microbes
containing chloroplast however they can also take in nutrition heterotrophically. The Euglena
has a red eyespot that detects light, permitting it to move in the direction of the light source.

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