NanoPhotometer®

Microvolume and Cuvette Spectroscopy

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 IMPLEN — A Global Acting Company

History
Founded in 2003, Implen has become a leading supplier for innovative spectroscopy instruments and consumables for
the non-destructive analysis of ultra-low volume liquid samples. The success story began with the introduction of the first
NanoPhotometer ® generation in 2006. Since then Implen has been providing best in class products offering unmatched
performance to its users supporting the work flows in modern research. Today thousands of biological, chemical,
pharmaceutical and forensic research organizations around the globe rely on Implen products.

Products & Services

Using patented technologies our products serve the demand for accurate and cost effective analysis for a wide range of
liquid samples and mobile applications. The compact designed products captivate by ease of use and proven reliability.
Implen’s NanoPhotometer® products are a forerunner in modern data communication and instrument control and provide
flexibility to its user which has never been available before. From our locations in Munich, Germany and Westlake Village,
California, we provide outstanding service to customers to answer technical questions and provide application support.

Core Values
We listen to our customers and design innovative products that provide
the highest benefit to our users. Implen’s highly qualified associates are
our most valued asset. As a team, we continuously apply our creativity
to the technologies of products, services, and processes. Continuous
improvement is our way of life. We constantly measure how well we
execute and define appropriate measures to do even better to achieve
outstanding customer satisfaction.
Martin Sahiri & Dr. Thomas Sahiri

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 Technology Made in Germany

Designed by German engineers, our NanoPhotometer® products will

exceed your expectations with regards to ease of use, functionality,
robustness and reliability over product life time.

Quality First. Always.

We are highly demanding towards the quality of our products and
services. Quality targets are implemented during the early product
design phase. Achieving them is verified throughout the entire
development process. Every NanoPhotometer® is fully tested before
being sent out to customers.

Implen’s Quality Management System is ISO 9001:2008 certified.

The NanoPhotometer® complies with IEC 61010-1, safety
requirements for electrical equipment for measurement, control, and
laboratory use.

Table of Contents

Technologies & Applications..............................4 - 5

NP80, N60/N50, C40......................................6 - 11

Feature Comparison / Accessories....................12 - 13

NP Operating System / Control Options.............14 - 15

Specifications..................................................16

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 ®
Unmatched NanoPhotometer Features

Battery Powered Sample Compression

Up to 8 hours battery TechnologyTM
operation Unique contained sample
environment

Flexible Unit Control

True Path TechnologyTM
Computer (Windows & Mac)
Built-in Touchscreen Optics providing exact path
Smartphone/Tablet (Android OS & iOS) lengths with two fixed anchor
points. No drift over lifetime

Windows is a trademark of Microsoft. Mac OS, OS X & iOS are trademarks of Apple. Android OS is a trademark of Google.

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 Sample Compression TechnologyTM- Accuracy & Precision

Reliable Protein Measurements

Reliable microvolume protein measurements are a challenge for most researchers due to a lack of surface tension
and a complex matrix of components in the samples. Implen’s Sample Compression TechnologyTM provides a reliable
measurement geometry for such challenging samples. A capillary film is formed between two quartz surfaces with each
sample eliminating the need for surface tension.

No Evaporation
Samples are completely enclosed in a stable micro-environment during the entire measurement process. Evaporation and
contamination of samples are reduced to a minimum. Even stable kinetic measurements in small volume samples are
possible as well as the determination of samples in volatile organic solvents.

Smallest Volume
®
Microvolume samples are measured in an advanced reflection mode on the NanoPhotometer . With 0.3 μl minimum
sample volume the NanoPhotometer is the instrument with the lowest volume requirement available!

True Path TechnologyTM

Simplicity is key for reliability. Implen’s True Path TechnologyTM covers the extensive dynamic range with only two precisely
defined path lengths. The sealed mechanical setup is providing two fixed anchor points that are not changing over the
lifetime of the instrument. No recalibration is necessary, Implen guarantees lifetime accuracy.

The unique Quartz based and metal free measurement environment is scratch resistant, inert, and does not require
surface reconditioning. It allows the use of a wide range of solvents (aqueous and organic) as well as buffers with higher
or lower pH values (an updated list of compatible solvents can be downloaded from www.implen.de).

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 ®
NanoPhotometer NP80
Microvolume & Cuvette

Accurate Mobile
Patented Sample Compression TechnologyTM with Spectroscopy can now be taken anywhere. The optional
guaranteed fixed path lengths allows for unmatched integrated battery pack provides up to 8 hours of stand-
accuracy and precision. Independent from surface alone battery powered operation.
tension and free from evaporation. Lifetime accuracy
guaranteed. Powerful
Built-in computer with a high performance Q7 quad core
Recalibration-free 1 GHz processor and 8 GB of onboard memory provides
Sealed optics without path length drift eliminates the rapid analysis and easy data storage.
need for costly and time consuming recalibrations.
Durable, inert surfaces provide easy to clean, scratch Fast
resistant surfaces that do not require reconditioning. Power on and instantly measure without lamp warm-up
(See also page 5.) time. Full scan capability from 200 - 900 nm provides
rapid and complete sample analysis in only 3.5 - 6 sec.
Easy
NPOS Operating System: intuitive graphical user
interfaces (GUI) providing one-step method access, pre-
programmed and customizable applications based on
the ultimate level of data security.

Flexible
Control your NanoPhotometer® via Touch Screen / Smart
Phone / Tablet / PC. (see page 15). Equipped with Wi-Fi,
USB A/B, HDMI, and LAN interface connections. Define
and store your own methods.

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 Exceeding Expectations

0.3μl Sample Volume

7” Color Touch Screen

Illuminated Sample Window

Temperature Controlled
Cuvette Holder

Power Indicator

Low Vibration Vortexer Easy to Clean Surfaces

Detection Limit Min Max

dsDNA 1 ng/μl 16,500 ng/μl

BSA 0.03 mg/ml 478 mg/ml

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 NanoPhotometer ® N60 / N50
Microvolume

Accurate Mobile
Patented Sample Compression Technology withTM
Spectroscopy can now be taken anywhere. The optional
guaranteed fixed path lengths allows for unmatched integrated battery pack provides up to 8 hours of stand-
accuracy and precision. Independent from surface tension alone battery powered operation.
and free from evaporation. Lifetime accuracy guaranteed.

Powerful
Recalibration-free Built-in computer with a high performance Q7 quad core
Sealed optics without path length drift eliminates the need 1 GHz processor and 8 GB of onboard memory provides
for costly and time consuming recalibrations. Durable, rapid analysis and easy data storage.
inert surfaces provide easy to clean, scratch resistant
surfaces that do not require reconditioning. (See page 5)
Fast
Power on and instantly measure without lamp warm-up
Easy time. Full scan capability from 200 - 900 nm (N50: 200
NPOS Operating System: intuitive graphical user interfaces - 650 nm) provides rapid and complete sample analysis
(GUI) providing one-step method access, pre-programmed in only 3.5 - 6 sec.
and customizable applications based on the ultimate level
of data security.

Flexible
Control your NanoPhotometer® via Touch Screen / Smart
Phone / Tablet / PC. (See page 15) Equipped with Wi-Fi,
USB A/B, HDMI, and LAN interface connections. Define
and store your own methods.

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 Best in its Class

Power Indicator

Illuminated Sample
Window

7” Color Touch Screen

0.3μl Sample Volume

Easy to Clean Surface

Low Vibration Vortexer

NanoPhotometer® N60 N50

Detection Limit Min Max Min Max

dsDNA 1 ng/μl 16,500 ng/μl 5 ng/μl 7,000 ng/μl

BSA 0.03 mg/ml 478 mg/ml 0.15 mg/ml 217 mg/ml

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 ®
NanoPhotometer C40
Cuvette

Powerful Flexible
Built-in computer with a high performance Q7 quad core Control your NanoPhotometer® via Touch Screen /
1 GHz processor and 8 GB of onboard memory provides Smartphone / Tablet / PC. (See page 15)
rapid analysis and easy data storage. Equipped with Wi-Fi, USB A/B, HDMI, and LAN interface
connections. Define and store your own methods.

Mobile
Spectroscopy can now be taken anywhere. The optional Fast
integrated battery pack provides up to 8 hours of stand- Turn on and instantly measure without lamp warm-up time.
alone battery powered operation. Full scan capability from 200 - 900 nm provides rapid and
complete sample analysis in only 3.5 sec.

Easy
NPOS Operating System: intuitive graphical user interfaces
(GUI) providing one-step method access, pre-programmed
and customizable applications based on the ultimate level
of data security.

Upgradable
The C40 NanoPhotometer® can be upgraded to a full
microvolume Spectrophotometer using Implen’s proven
Submicroliter cell which utilizes its patented Sample
Compression TechnologyTM. Being able to measure 0.3 μl
samples you will have access to comprehensive life
science methods and application detecting concentrations
from 2 ng/μl up to 18.750 ng/μl.

10

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 More than just a Cuvette Spectrophometer

7” Color Touch Screen

Temperature Controlled
Cuvette Holder

Detection Limit Min Max

dsDNA 0.100 ng/μl 130 ng/μl

BSA 0.003 mg/ml 3.7 mg/ml

11

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 NanoPhotometer® Model Comparison

NP80 N60 N50 C40

Model

Nanovolume + Nanovolume Nanovolume Cuvette

Cuvette

Full Spectrum Scan 200 – 900 nm 200 – 900 nm 200 – 650 nm 200 – 900 nm

Detection Range dsDNA: 1-16,500 ng/μl dsDNA: 1-16,500 ng/μl dsDNA: 5-7,500 ng/μl
N/A
Nanovolume BSA: 0.03-478 mg/ml BSA: 0.03-478 mg/ml BSA: 0.15-217 mg/ml

Detection Range dsDNA: 0.1-130 ng/μl dsDNA: 0.1-130 ng / μl

N/A N/A
Cuvette BSA: 0.003-3.7 mg /ml BSA: 0.003-3.7 mg /ml

Path lengths 0.67 and 0.08 mm 0.67 and 0.08 mm 0.67 and 0.08 mm 10 mm

Touch Screen Optional Optional Optional Optional

Customizable Methods

Built-in Cuvette

Built-in Vortex

Built-in Battery Pack Optional Optional Optional

12

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 Accessories

Field Kit
Leave the lab bench behind ... start exploring!
The NanoPhotometer® field kit is your mobile screening lab. With
space for pipette, sample and buffer containers, accessories and
documentation the limitations of a lab are eliminated. With up to 8
hours of battery power there is no need to worry about a power outlet.

The convenient NanoPhotometer® roller case is specially designed to

fit into the overhead bins on most major airlines. To comply with TSA
requirements the case can be equipped with a TSA lock for safe airline
transportation.

TM
DiluCell
DiluCellTM is especially designed for use with the NanoPhotometer® and
OD600 DiluPhotometerTM for the analysis of bacterial and yeast cultures and
Bradford protein assays. Due to the reduced path length DiluCellTM provides an
automatic dilution without the need of a physical dilution of higher concentrated
samples. The two different available versions DiluCellTM 10 and DiluCellTM 20
allow an automatic 1/10 and 1/20 dilution of the sample. Bypassing manual
sample dilutions reduces dilution errors and cross contamination making
DiluCellTM ideal for GLP. Combined with small sample volume requirements
and bubble free filling, the DilucellTM allows for convenient spectrophotometric
analysis from 340 - 900 nm.

13

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 NPOS

The most powerful operating system in UV/Vis spectrophotometry provides flexibility and mobility with its web based
interface running on multiple devices (computer, tables and smartphones) and systems (Windows and Mac). Pre-
programmed and customizable applications with intuitive interfaces and one-step method access for easy data analysis
and data/method saving. The Linux based NPOS (NanoPhotometer® Operating System) also provides the ultimate level of
data security.

The innovative graphical user interface (NPOS GUI) allows

easy touch and/or conventional access to all parameters.
Results can be edited, exported and graphically overlaid.
The most advanced GUI in history of lab equipment –
combined with ease of use at its best. The software puts
the focus on what is the most important:
The measurement itself.

14

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 Flexible Unit Control

Smart Phone LCD Touchscreen

Tablet PC/Mac

Mobile and easy control of the NanoPhotometer® via Wi-Fi from any tablet or smartphone (Android OS & iOS). Easy control
from any PC (Windows & Mac) with fast and flexible connection options including Wi-Fi, USB, HDMI and LAN. Optional 7”
built-in color LCD touchscreen with glove compatible touch technology.

15

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 Technical Specifications
NanoVolume Performance Zero Stability ±0.003 A/hour after 20min warm up @ 260 nm

Detection Range dsDNA 1 ng/μl to 16,500 ng/μl Noise 0.002 A rms at 0 A @ 260 nm
(N50: 5 ng/μl to 7,500 ng/μl) 0.002 A (pk to pk) at 0 A @ 260 nm

Detection Range BSA 0.03 mg/ml to 478 mg/ml Optical Arrangement 1 x 3648 CCD Array
(N50: 0.15 mg/ml to 217 mg/ml) (N50: 1 x 1024 CCD Array)

Minimum Sample Size 0.3 μl Lamp Xenon flash lamp

Photometric Range 0.02 - 330 A Lifetime 109 flashes, up to 10 years

(10 mm equivalent) (N50: 0.1 - 150 A)
Processing Power & Compatibility
Path Length 0.67 and 0.07 mm
Operating System Linux based NPOS
Dilution Factor 15 and 140
Onboard Processor Quad Core 1 GHz
Vortexer 2,800 rpm; tube size up to 2.0 ml
Internal Storage 8 GB
Cuvette Performance
Control Options Onboard with built-in touch screen, computer,
Detection Range dsDNA 0.1 ng / μl to 130 ng / μl smart phone and tablet

Detection Range BSA 0.003 mg / ml to 3.7 mg / ml Software Compatibility Windows 7 (32 & 64 bit), Windows 8 (32 & 64
bit), OS X, iOS & Android OS
Photometric Range 0 - 2.6 A
Min. Requirement 4 inch screen; Apple: iPad 2, iPhone5 &
Center Height (Z-Height) 8.5 mm Smart Phone/Tablet iOS 6; Android OS version 2.2, Quadcore 1.2
GHz with 1 GB RAM
Cell Types Outside dimension 12.5 x 12.5 mm
General Specifications
Heating 37°C ± 0.5°C
Main Body Size 200 mm x 200 mm x 120 mm
Optical Specifications

Features and specifications are subject to change without notice.

Weight 3.8 - 5.2 kg depending on configuration
Wavelength Scan Range 200 – 900 nm (N50: 200 - 650 nm)
Operating Voltage 90-250 V, 50/60 Hz, 60 W (90 W with
Measure Time For Full 3.5 - 6.0 seconds
battery pack), 18/19 VDC
Scan Range
Display 1024 x 600 pixels; Touch-Screen glove
Wavelength ± 0.2 nm (N50: ± 1nm)
compatible
Reproducibility
Built-in Battery Pack Optional rechargeable lithium ion battery;
Wavelength Accuracy ± 0.75 nm (N50: 1.5 nm)
95Wh, 6,6Ah;
Bandwidth better than 1.8 nm (N50: 5 nm) Operation time: up to 8 h; min. charging
cycles: 800
Stray Light < 0.5 % at 220 nm using NaI and < 1% at
280 nm using Acetone Battery Certification IEC 62133 and UN38.3 transport test

Absorbance < 0.002 A (0.67 mm path) @ 260 nm In & Output Ports 2x USB A, USB B, HDMI, Ethernet, WLAN
Reproducibility (N50: 0.004 A (< 0.67 mm path) @ 260 nm)
Additional Data Input Mouse & keyboard options
Absorbance Accuracy < 1.75 % @ 0.7 A (0.67 mm path) @ 260 nm
of the reading Security Slot for Kensington lock

www.implen.de | www.implen.com
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