PREBOARD 1 EXAMINATION SAMPLE PAPER (2024-25)

Subject: BIOLOGY Max. Marks:70

Grade: XII Time: 3 Hrs
Name: Section: Roll No:
General Instructions:

i) All questions are compulsory

ii) The question paper has 5 sections and 33 questions. All questions are compulsory
iii) Section A has 16 questions of 1 mark each. Section B has 5 questions of 2 marks each; section
C has 7 questions of 3 mark each ; section D has 2 case based questions of 4 marks
each ;section E has 3 questions of 5 marks each
iv) There is over all choice . However , internal choices have been provided in some questions. A
student has to attempt only one of the alternatives in such questions
v) Wherever necessary ,neat and properly labeled diagrams should be drawn
.
SECTION A
1. If Meselson and Stahl's experiment is continued for sixth generations in bacteria, the ratio of Heavy
strands 15N/15N :Hybrid15N/14N : light 14N/14N containing DNA in the sixth generation would
be
a. 1:1:1 b. 0:1:7

c. 0:1:15 d. 0:1:31

2. What are minisatellites ?

a. 10-40 bp sized small sequences within the b. Short coding repetitive region on the
genes eukaryotic genome
c.
Short non-coding repetitive sequence d. Regions of coding strands of the DN
forming large portion of eukaryotic genome
3. The human chromosome with the highest and least number of genes in them are respectively
a. Chromosome 21 and Y b. b) Chromosome 1 and X

c. (c) Chromosome 1 and Y d. Chromosome X and Y

Page 1 of 8
4 In a cereal grain, the single cotyledon of embryo is represented by

a. coleoptile b. coleorhiza

c. scutellum d. hypocotyl

5 There is a restriction endonuclease called BamHI . What does “am” part in it stand for?
a. amyloliquefaciens b. amylose
c. amide d. ampicillin

6 A cross is made between tall pea plants having green pods and dwarf pea plants having yellow
pods. In the F2 generation, out of 80 plants how many are likely to be tall plants?

a. 15 b. 20

c. 45 d. 60

7 Peptide A and peptide B is linked by how many disulphide linkages in insulin . ,

a. 1 b. 2

c. 3 d. 4

8 At a particular locus, the frequency of allele A is 0.8 and that of allele a is 0.2. What would be
the frequency of heterozygotes in a random mating population at equilibrium?

a. 0.32 b. 0.16
c. 0.24 d. 0.48

9 A patient was advised to have a kidney transplant. To suppress the immune reaction, the
doctor would administer him:

a. statins produced from Monascus purpureus b. statins produced from Streptococcus

thermophilus
c. cyclosporin A produced from Trichoderma d. cyclosporin A produced from Clostridium
polysporum butylicum
10 .
Given below is a flow chart showing ovarian changes during menstrual cycle. Fill in the space
a with the hormonal factor(s) responsible for the events shown.

Page 2 of 8
 a. FSH and estrogen b. LH and FSH

c. ACTH and LH d. Progesterone and LH

11 From the sexually transmitted diseases mentioned below, identify the one which does not
specifically affect the sex organs ,

a. Syphilis b. AIDS

c. Gonorrhea d. Genital warts

12 Study the pedigree chart given below. What does it show?

a. Inheritance of a condition like b. The pedigree chart is wrong as this is not

phenylketonuria as an autosomal recessive possible.
trait.
c. Inheritance of a recessive sex-linked disease d. Inheritance of a sex-linked inborn error of
like haemophilia metabolism like phenylketonuria.

Question No. 13 to 16 consists of two statement Assertion (A) and reason (R) .Answer these
questions selecting the appropriate option given below

(a) both A and R are true and R is the correct explanation of A.

(b) both A and R are true but R is not the correct explanation of A.
(c) A is true but R is false.
(d) A is false but R is true
13 Assertion : Hypocotyl develops into radicle on root tip.
Reason : A dicot embryo consist of two cotyledons.

14 Assertion:Ribosomal RNA is synthesized in the nucleus of the cell.

Reason: It is translated with the enzyme RNA polymerase III.

Page 3 of 8
15 Assertion: PCR is a powerful technique to identify genetic disorders.
Reason: PCR can detect mutations in low amounts of DNA
16 Assertion : Stem cells are undifferentiated biological cells found in multicellular organisms.
Reason : They are obtained from only umbilical cord blood just after birth.

SECTION B

17 In an effort to mass produce a useful protein, scientists extracted a precise section of mRNA from
the cytoplasm of cells which naturally produced the protein. The mRNA strand was then used as a
template to enzymatically synthesise a strand of complementary DNA (cDNA).

18 Observe the graph given below. The graph represents inter-specific interaction between two species
of Paramecia competing for the same resource in a culture medium. Paramecium caudatum and
Paramecium aurelia were grown in separate cultures as well as in mixed cultures. It was found that
each species grew in numbers according to the logistic equation.
a) Which species is competitively superior? Support it with the data provided in the graph. b)
State the underlying principle for the above result and name the scientist associated with this
principle. c) Explain the mechanism in which two or more species competing with each other can
co exist

Page 4 of 8
19 Kavya says that the placenta produces relaxin which plays a crucial role during pregnancy. (a) Is
she correct? Justify. (b) Name TWO other hormones secreted by the placenta during pregnancy
20 Explain any TWO reasons why the treatment of AIDS is only partially effective
21 The following photograph shows the result of a technique showing the separation of DNA.

a) Name the technique.

b) DNA fragments of size 500 bp, 1600 bp and 2000 bp are separated by this process. Which
fragment will migrate fast? Why?
OR

Do eukaryotic cells have restriction endonucleases? Justify your answer.

SECTION C
22 DNA separated from one cell, when introduced into another cell is able to bestow some of the
properties of former to the latter. What is this change called in technical terms? Describe the
experimental evidences which led to the discovery of the above phenomenon
23 Evaluate the suitability of DNA and RNA as genetic material and justify the suitability of the one
that is preferred as an ideal genetic material

24 What is Ti plasmid? Name the organism where it is found. How does it help in genetic
engineering?

25 a) List any two characteristic features of wheat flowers that make it a good example of wind
pollination.
(b) It is observed that plant breeders carrying out wheat hybridisation often take pollen grains
from the ‘pollen banks’. Do you agree ? Give one reason in support of your answer.

26 The figure below shows the sequence of changes within the ovary that occur during the menstrual
cycle.

Page 5 of 8
 a) Name the process A. Name the hormone that plays an important role during this event. b)
Identify B and name the hormone that regulates the maturation of B. c) Identify and write the
function of C
27 In a huge culture flask with unlimited supply of nutrient medium, bacteria were grown. Their
population kept on increasing as they were dividing by binary fission. a) What type of growth
pattern will be seen in this population? b) Write the equation which can be used to calculate the
population size after time t, when the initial population size of the bacteria is represented by N and
population size after time t is represented by Nt. c) Draw a growth curve to depict the growth in the
population size when growth in the population size is plotted over time. What will be the shape of
this growth curve?
28 (i) Why is it important to measure biochemical oxygen demand (BOD) of the effluent? At what
stage of sewage treatment is this testing done? (ii) BOD level of three samples of water labelled as
A, B and C are 60 mg/L, 20 mg/L and 500 mg/L respectively. Which sample of water is most
polluted?
SECTION D
29 The data below shows the concentration of nicotine smoked by a smoker taking 10 puffs/ minute.

a) With reference to the above graph explain the concentration of nicotine in blood at 10 minutes.
(b) How will this affect the concentration of carbon monoxide and haembound oxygen at 10
minutes? (c) How does cigarette smoking result in high blood pressure and increase in heart rate?
OR (c) How does cigarette smoking result in lung cancer and emphysema?

.30 Write the characteristics of Ramapithecus, Dryopithecus, Australopithecus and Neanderthal man.

SECTION E
Page 6 of 8
31 A woman was unable to conceive after many years of regular unprotected coitus went to
specialised infertility clinic . On complete examination woman was found to be normal while male
partner was diagnosed with infertility. . the male partner can inseminate normally but the mobility
of sperms is below 40 percent. Couple was advised to opt for assited reproductive technology.
i) Which ART could have been useful for this couple ?
a) ZIFT b) IUT c )ICSI d) GIFT
ii) which among the folloing techniques is suggested for correcting infertility caused due
to very low sperm counts of a male partner?
a) GIFT b) Test tube baby c) IUT d) AI
iii) A large number of married couples the world over are childless. It is shocking to know
that in India the female partner is often blamed for the couple being childless.
(a) Why in your opinion the female partner is often blamed for such situations in India?
(b) State any one reasons responsible for the cause of infertility.

32 Study the schematic representation of the genes involved in the lac operon given below and
answer the questions that follow:

p i p o z y a

(i) Identify and name the regulatory gene in this operon. Explain its role in ‘switching off’ the
operon.
(ii) Why is lac operon’s regulation referred to as negative regulation?
(iii) Name the inducer molecule and the products of the genes ‘z’ and ‘y’ of the operon. Write
the functions of these gene products

OR
Answer the following questions based on Hershey and Chases’s experiments:
(a) Name the kind of virus they worked with and why?
(b) Why did they use two types of culture media to grow viruses in? Explain.
(c) What was the need for using a blender and later a centrifuge during their experiments?
(d) State the conclusion drawn by them after the experiments.

Read the following text and answer questions from Q.1. to Q.5. given below :
33 Plants having foreign genes in their genome through genetic engineering are called transgenic
plants. Genes can be incorporated either through a vector or through direct introduction of DNA. Bt
cotton is a genetically modified organism which is pest resistant. It contains gene cry I Ac and cry
II Ab of Bacillus thuringiensis. It is used to control lepidopterans, coleopterans and dipterans. Bt
cotton can resist cotton bollworm and produce higher yields. Cry gene produces cry protein or Bt
toxin. It is an endotoxin which remains as protoxin in plants and converted to active toxin after
getting ingested by the insects. Alkaline pH of the insect gut solubilizes the protein crystals, the
activated toxin creates pores to the mid gut wall of the insects which cause them to death
a) What is special about Bt cotton crops?
Page 7 of 8
 b) Write about the genes present in Bt cotton and its function
c) What are the advantages of GM plants
d) What is Bacillus thuringiensis?
e) What are lepidopterans?
OR
Read the following text and answer the following questions on the basis of the same :
Transgenic animals have their DNA manipulated to possess and express an extra gene. The genome
of these animals has been changed and they can carry genes from other species. The first transgenic
sheep was Tracy. The reason behind producing these transgenic animals are for specific economic
trait, some are produced as disease models (animals genetically manipulated to exhibit disease
symptoms so that effective treatment can be studied)
(a) Who developed the first transgenic animal?
(b) 95% transgenic animals are ______
(c) Name the first cloned animal and the cell from which it was cloned?
Name the first transgenic cow,the year in which it was produced and its significance.

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

Page 8 of 8