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Lab 3 - Assignment - Membranes. - F2024

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11 views7 pages

Lab 3 - Assignment - Membranes. - F2024

Uploaded by

emmanuella.akhigbe
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Biol 1010U – FALL 2024

EXPERIMENT # 3:
Membrane structure and function

Laboratory assignment

Name: ________________________________

ID #: ________________________________

TA’s name: ________________________________

Lab CRN #: ________________________________

Your lab report is due at the end of your lab session.

Please note that some parts of the assignment (highlighted in red


on pages 2 and 4) have to be completed before the lab as part of
the lab assignment following instructions found in the protocol
section.
If you haven’t completed these questions before the lab, you will
not be allowed in the lab and you will receive zero on all
assignments associated with the lab.

Please write your answers in the space provided. Anything written outside the
lines will not be corrected.

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Exercise 2: Preparation of the standard curve for betacyanin

Table 1: Dilution of betacyanin stock solution (9 µM) for preparation of the


standard curve. Fill in columns 4 and 5 before the lab as part of the
lab assignment.

Column 1 Column 2 Column 3 Column 4 Column 5 Column 6


Solution Volume of Volume of Dilution Concentration
Number betacyanin distilled factor of diluted A525
(mL) water sample (µM)
(mL)
1 5.0 0
2 4.0 1.0
3 3.0 2.0
4 2.5 2.5
5 2.0 3.0
6 1.0 4.0
7 0 5
Stock concentration of betacyanin: 9 µM
Show your calculations and the formulas used below for the calculation of
the dilution factors and concentrations of diluted samples. This must
be completed before the lab as part of your lab assignment.

My blank solution is in Tube #: ______________________________

2|Page
Once you have obtained the absorbance at 525 nm (A 525; column 6 of
Table 1), draw the standard curve on the graph paper provided. Make sure
you properly label the axes and include the figure number and a figure title.

Exercise 3: Effect of temperature on membrane integrity

Table 2: Absorbance of betacyanin leaked from beet root discs exposed to


different temperatures. Column 3 to 5 are suggested times for the
experiment.

1 2 3 4 5 6 7
Tub Treatme Treatme Treatmen Water A525 Concentratio
e# nt nt IN t OUT beaker n (µM)**
(oC) (min)* (min)* OUT
(min)*
A -20 0 3 8
B 22oC 1 4 9
C 60oC 2 5 10
*From timer counting up.
**Using the standard curve and the absorbance values obtained (column 6),
determine the concentration of betacyanin in the water.

Using the graph paper provided, draw a bar graph showing the relationship
between the concentration of betacyanin released and temperature.

3|Page
Answer the following questions in the space provide (anything written
outside the space will not be corrected):

1. According to your experiment, which temperature(s) were the most


damaging to the membrane?

2. According to your experiment, which temperature(s) had minimal or no


effect on membranes?

3. How do high temperatures damage membranes? Briefly explain your


answer, incorporating theory of the Fluid Mosaic Model. (Answer
before the lab as part of the lab assignment)

4. How does freezing damage membranes? Briefly explain your answer,


incorporating theory of the Fluid Mosaic Model. (Answer before the
lab as part of the lab assignment)

5. Are your experimental results in accordance with your answers in


questions 3 and 4? Please comment.
4|Page
6. Throughout the experiment, the spectrophotometer was set at 525
nm. Explain why this wavelength was used.

7. Your TA gives you a solution of chlorophyll, the green pigment found in


plant cells. Can you use the standard curve for betacyanin to
determine the concentration of chlorophyll in this solution? Briefly
explain why or why not.

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