Vivaspin Turbo 4 15 Pes RC Datasheet en L Sartorius PDF Data

Product Datasheet
Vivaspin ®
Turbo 4 and 15
PES and RC
Enabling the Fastest
Sample Concentration with
Highest Recoveries
---
Benefits Product Information
--
Complete Recoveries Vivaspin® Turbo 4 and 15 PES and RC centrifugal
Fastest Concentrations concentrators offer the optimal solution to any
Highest Chemical Resistance concentration or buffer exchange application with their
Unique PES and RC Device Range broad range of MWCOs and unique membrane choice.
Optimal Sample to Membrane Compatability
Highest flow rates are achieved due to their twin vertical
membranes which minimize protein polarization and
subsequent fouling of the membrane. Additionally, their
sleek internal profile ensures maximum process speeds
right down to the last 100 µL.
Applications
--
Working Principle
Centrifugation provides the vector to clear solvent and
microsolutes through an ultrafiltration membrane, enabling Sample preparation
-
separation of macromolecular species from solvents Sample (protein, lipid, exosomes, virus, nanoparticle,
primarily on the basis of size. It is particularly appropriate for macromolecule) concentration
the concentration of macromolecules and can also be used Desalting | dialysis
to purify molecular species or for solvent exchange. Buffer exchange
Ultrafiltration is a gentle, non-denaturing method that is
more efficient and flexible than alternative processes. Your sample is often the result of several months of
research. It is valuable, and the Vivaspin® Turbo range
High Performance provides the highest recoveries.
In a single spin, 4 or 15 mL solutions can be concentrated up
to 150×. Samples can be typically concentrated in 10–30 Summary
minutes with macromolecular recoveries in excess of 90%. For scientists and lab technicians who need to quickly and
safely concentrate a wide range of biological samples of
Optimized Design for Fastest Concentration Results 2 mL to 15 mL initial volume up to 150-fold, Sartorius offers
The Vivaspin® Turbo optimized design with sleek internal the Vivaspin® Turbo 4 and 15 PES and RC ultrafilters.
profile ensures maximum process speeds all the way down
to the last few microlitres. UV and ultrasonic joining Unlike alternative ultrafiltration units, Vivaspin® Turbo is
technologies provide a smooth transition between equipped with an angular dead-stop pocket, that enables
membrane and plastic housing, allowing all of your valuable reproducible and complete recoveries, while being the
sample to be collected from the unique, pipette friendly fastest in the market.
angular dead-stop pocket.
Now with the choice between both PES and RC membrane
variants, the Vivaspin® Turbo range enables users to
The ultimate in centrifugal ultrafiltration
--
choose the best membrane for their target, in the same
technology super-fast device.
Sleek internal design: Ensures maximum process speed
-
for complete ultrafiltration
Large twin vertical membranes: Avoids fouling of the
membrane thanks to minimized protein polarization.
Unique angular dead stop pocket: Eliminates the risk of
the sample concentrating to dryness, whilst being easily
accessible to standard 200 μL pipette tips for convenient
and complete recovery of the concentrate.
Optimized Choice of Materials for

High Chemical Compatibility
The combination of polyethersulfone (PES) | regenerated
cellulose (RC), polystyrene and polypropylene (PP) allows
sterilization and depyrogenization of the Vivaspin® Turbo units.
PES membranes are preferred for their low fouling

characteristics, exceptional flux and broad pH range
compatibility.
RC membranes offer a neutral membrane charge and broad

chemical compatibility well suited to general sample types.
2
Technical specifications
Vivaspin® Turbo 4 PES Vivaspin® Turbo 15 PES Vivaspin® Turbo 15 RC
Materials Body Styrene butadiene Styrene butadiene Styrene butadiene

copolymer copolymer copolymer
Filtrate vessel Polypropylene Polypropylene Polypropylene
Concentrator cap Polypropylene Polypropylene Polypropylene
Membrane Polyethersulfone Polyethersulfone Regenerated Celloluse
Dimensions Total length, concentrator insert 75.5 mm 77 mm 77 mm
Total length, assembled 122.5 mm 118 mm 118 mm
Diameter, concentrator insert 14.6 mm 27 mm 27 mm
Active membrane area 3.2 cm2 7.2 cm2 8.1 cm2
Hold up volume, membrane <10 µL <10 µL <10 µL
Dead-stop volume. swing out 40 µL 100 µL 100 µL
Dead-stop volume, fixed angle 30 µL 60 µL 60 µL
Concentrator capacity Swing bucket rotor 4 mL 15 mL 15 mL
Fixed angle rotor (25°) 4 mL 9 mL 9 mL
Maximum speed 3 kDa – 50 kDa Swing bucket rotor 4,000 g 4,000 g 4,000 g
Fixed Angle rotor (25°) 7,500 g 4,000 g 6,000 g
Maximum speed 100 kDa Swing bucket rotor 3,000 g 2,000 g 3,000 g
Fixed Angle rotor (25°) 5,000 g 2,000g 6,000 g
Sterilization EtO or 70% EtOH
Removal of endotoxins 1N NaOH*

[Depyrogenization]
* PES variants only
3
Performance Characteristics
Vivaspin® Turbo 4 PES
Time to concentrate up to 30× at 20°C and solute recovery
Rotor Swing bucket Fixed angle (25°)
Centrifugal force 4,000 g 7,500 g
Start volume 4 mL 4 mL
Time Recovery Time Recovery
Cytochrome c* (12.4 kDa)

3 kDa MWCO PES 60 min. 98% 80 min. 96%
Lysozyme* (14.3 kDa)

α-Chymotrypsin** (25 kDa)

BSA** (66 kDa)

IgG** (160 kDa)

100 kDa MWCO PES**** 17 min. 94% 13 min. 92%
Vivaspin® Turbo 15 PES

Cytochrome c* (12.4 kDa)



BSA** (66 kDa)

IgG** (160,000 MW)

100 kDa MWCO PES*** 30 min. 92% 16 min. 92%
4
Vivaspin® Turbo 15 RC

5 kDa MWCO RC 23 min. 94% 25 min. 92%

BSA** (66 kDa)

IgG** (160 kDa)

100 kDa MWCO RC**** 18 min. 89% 11 min. 89%
* 0.25 mg/mL
** 1 mg/mL
*** 3,000 g centrifugal speed
**** 3,000 g swing-out | 5,000 g fixed angle
***** 3,000 g swing-out | 4,000 g fixed angle
Ordering Information
Vivaspin® Turbo 4 and 15 centrifugal concentrators, disposable ultrafiltration units, for processing sample volumes
of 2 – 4 mL and 15 mL, Polyethersulfone UF membrane.
Vivaspin® Turbo 4 PES Vivaspin® Turbo 15 PES Vivaspin® Turbo 15 RC

Cut off Quantity Order no. Cut off Quantity Order no. Cut off Quantity Order no.
3 kDa MWCO 25 VS04T91 3 kDa MWCO 12 VS15T91
3 kDa MWCO 100 VS04T92 3 kDa MWCO 48 VS15T92
5 kDa MWCO 25 VS04T11 5 kDa MWCO 12 VS15T11 5 kDa MWCO 12 VS15TR11
5 kDaMWCO 100 VS04T12 5 kDa MWCO 48 VS15T12 5 kDa MWCO 48 VS15TR12
5
Sales and Service
Contacts
For further contacts, visit
www.sartorius.com
Germany USA
Sartorius Lab Instruments Sartorius Corporation
GmbH & Co. KG 565 Johnson Avenue
Otto-Brenner-Strasse 20 Bohemia, NY 11716
37079 Goettingen Phone +1 631 254 4249
Phone +49 551 308 0 Toll-free +1 800 635 2906
Specifications subject to change without notice.

Printed and copyrighted by Sartorius Stedim Biotech GmbH.
Status: 08 | 2021

Vivaspin Turbo 4 15 Pes RC Datasheet en L Sartorius PDF Data

Uploaded by

Copyright:

Available Formats

Vivaspin Turbo 4 15 Pes RC Datasheet en L Sartorius PDF Data

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Vivaspin Turbo 4 15 Pes RC Datasheet en L Sartorius PDF Data

Uploaded by

Copyright:

Available Formats

Product Datasheet

Sleek internal design: Ensures maximum process speed

Optimized Choice of Materials for

PES membranes are preferred for their low fouling

RC membranes offer a neutral membrane charge and broad

Materials Body Styrene butadiene Styrene butadiene Styrene butadiene

Filtrate vessel Polypropylene Polypropylene Polypropylene

Concentrator cap Polypropylene Polypropylene Polypropylene

Membrane Polyethersulfone Polyethersulfone Regenerated Celloluse

Dimensions Total length, concentrator insert 75.5 mm 77 mm 77 mm

Total length, assembled 122.5 mm 118 mm 118 mm

Diameter, concentrator insert 14.6 mm 27 mm 27 mm

Active membrane area 3.2 cm2 7.2 cm2 8.1 cm2

Hold up volume, membrane <10 µL <10 µL <10 µL

Dead-stop volume. swing out 40 µL 100 µL 100 µL

Dead-stop volume, fixed angle 30 µL 60 µL 60 µL

Concentrator capacity Swing bucket rotor 4 mL 15 mL 15 mL

Fixed angle rotor (25°) 4 mL 9 mL 9 mL

Fixed Angle rotor (25°) 7,500 g 4,000 g 6,000 g

Fixed Angle rotor (25°) 5,000 g 2,000g 6,000 g

Sterilization EtO or 70% EtOH

Removal of endotoxins 1N NaOH*

* PES variants only

Rotor Swing bucket Fixed angle (25°)

Centrifugal force 4,000 g 7,500 g

Time Recovery Time Recovery

Cytochrome c* (12.4 kDa)

Lysozyme* (14.3 kDa)

α-Chymotrypsin** (25 kDa)

BSA** (66 kDa)

IgG** (160 kDa)

Vivaspin® Turbo 15 PES

Rotor Swing bucket Fixed angle (25°)

Centrifugal force 4,000 g 4,000 g

Time Recovery Time Recovery

Cytochrome c* (12.4 kDa)

Lysozyme* (14.3 kDa)

α-Chymotrypsin** (25 kDa)

BSA** (66 kDa)

IgG** (160,000 MW)

Rotor Swing bucket Fixed angle (25°)

Centrifugal force 4,000 g 7,500 g

Time Recovery Time Recovery

Lysozyme* (14.3 kDa)

α-Chymotrypsin** (25 kDa)

BSA** (66 kDa)

IgG** (160 kDa)

Vivaspin® Turbo 4 PES Vivaspin® Turbo 15 PES Vivaspin® Turbo 15 RC

3 kDa MWCO 25 VS04T91 3 kDa MWCO 12 VS15T91

3 kDa MWCO 100 VS04T92 3 kDa MWCO 48 VS15T92

5 kDa MWCO 25 VS04T11 5 kDa MWCO 12 VS15T11 5 kDa MWCO 12 VS15TR11

5 kDaMWCO 100 VS04T12 5 kDa MWCO 48 VS15T12 5 kDa MWCO 48 VS15TR12

10 kDa MWCO 25 VS04T01 10 kDa MWCO 12 VS15T01 10 kDa MWCO 12 VS15TR01

30 kDa MWCO 25 VS04T21 30 kDa MWCO 12 VS15T21 30 kDa MWCO 12 VS15TR21

50 kDa MWCO 25 VS04T31 50 kDa MWCO 12 VS15T31 50 kDa MWCO 12 VS15TR31

Specifications subject to change without notice.

You might also like