Gen Bio - Q2 - M2 Final
Gen Bio - Q2 - M2 Final
Department of Education
Regional Office IX, Zamboanga Peninsula
11
Zest for Progress
Z eal of Partnership
GENERAL BIOLOGY 1
Quarter 2 – Module 2
Electron Flow in Light
Dependent Reaction and
Calvin Cycle
What makes plants green? A small molecule with a big job called chlorophyll is responsible
for this, and it's also what helps plants get energy from the sun. Chlorophyll is a light-absorbing
pigment, and it actually gets its green color because it absorbs blue and red wavelengths of light.
The green wavelengths are reflected, giving that unmistakable color to plants. Since it is a light-
absorbing pigment, chlorophyll is called a photoreceptor. Chlorophyll molecules are contained inside
chloroplasts, which are the food producers of the cell found in all green parts of a plant. Inside the
chloroplasts, we also find thylakoid membranes, which contain photosystems. Photosystems are
made of a group of light-harvesting complexes, which is just a fancy term for pigment molecules and
proteins. Two photosystems have been identified: photosystem I and photosystem II. The
chlorophyll molecules are arranged in and around the photosystems, and this allows them to transfer
the light energy into the center of the photosystem. This light energy comes from photons. Photons
are one way that light travels, as discrete packets of energy. Thus, there must be a significant
process in plants that used up photons significantly.
What’s In
Pigments are molecules that absorb specific wavelengths (energies) of light and reflect all others.
Pigments are colored: the color we see is the net effect of all the light reflecting back at us. Now, what is
meant by the absorption?
Electrons in molecules can exist at specific energy levels. However, if enough energy comes along
to boost them into the next level, they can "absorb" that energy and occupy that higher level. This is what
pigments do. The light they absorb contains' just the right amount' of energy necessary to push them into
the next level. Any light that does not have enough or has too much energy cannot be absorbed and is
reflected.
ACTIVITY 1: RECALL:
Score:
Parts of the Electromagnetic spectrum _____
Directions: Label the different parts of the Electromagnetic Spectrum(Figure1) below. Choose your
answer from the terms provided on the table before figure 1
ü Radio wave
ü Ultraviolet
ü Visible light
ü Infrared
Radiation
ü Microwave
ü X-Rays
ü Gamma Rays
Inside the leaf is a site of food production in plants where it was able to create glucose for
plants to grow and survive called the chloroplast.
Knowing that light has pigments and some of it is harnessed by plants for photosynthesis, what
should be the ideal pigment for chloroplasts?
A collection of pigments that would absorb all light and thus appear Black seems a logical choice...
but in fact we know this is not true. Plants except for some of the red algae appear green or brown,
not black. Why? a number of possible explanation occur...
If plants had pigments that absorbed UV and x-rays this would mean that so much energy could be
absorbed in light areas that electrons could be knocked off their orbitals and the molecule destroyed.
If plants absorbed IR and radio waves, there would not be enough energy for electron transfer, just
enough to warm up the molecule
Pigments that absorb in the visible region gain just enough energy to boost an electron to the next
level.
Directions: Match each pigment involved in photosynthesis below to its specified description. Write
the letter of your chosen answer in the space provided before each number.
COLUMN A COLUMN B
____ 1. Most abundant pigment in plants, reflects green A. Phycocyanin
light strongly so it appears green to us
____ 2. It absorbs light of 453nm and 642 nm maximally B. Xanthophylls
and helps increase the range of light a plant can use
for energy.
____ 3. Absorbs light maximally between 460 nm and 550 C. Carotenoids
nm and appear red, orange, or yellow to us.
____ 4. They are usually red and yellow and do not absorb D. Anthocyanins
energy as well as carotenoids. They are also fat
soluble.
____ 5. These pigments contain Cu and are stored in the E. Chlorophyll b
vacuole of a plant because they unlike the other fat
soluble pigments are water soluble.
6. Present in red and blue algae. F. Chlorophyll a
What’s New
2. Which photosystem is first hit by photons allowing reaction flow? Why does it start there?
_______________________________________________________________________________________
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What Can I Do
3. Of what importance does the electron transport chain plays in the light dependent reaction?
_______________________________________________________________________________________
_______________________________________________________________________________________
_______________________________________________________________________________________
4. What does water molecule pays in the light dependent reaction specifically in the production ATP and
NADPH?
_______________________________________________________________________________________
_______________________________________________________________________________________
_______________________________________________________________________________________
What Is It
In this learning module, we’ll evaluate how photons were able to energized electrons in light
dependent reactions causing them to escape and result to the production of six-carbon molecule, glucose as
a product of the Calvin Cycle.
What happens in the electrons in the light independent reaction?
The light dependent reactions use photosynthetic pigments (organized into photo systems) to convert light
energy into chemical energy (specifically ATP and NADPH).
These reactions occur within specialized membrane disc within the chloroplast called thylakoids and involve
three steps:
ü Excitation of photosystems by light energy
ü Production of ATP via an electron transport chain
ü Reduction of NADP+ and photolysis of water.
ü
Understanding:
ü Absorption of light by photosynthesis generates excited electrons.
ü Transfer of excited electrons occurs between carriers in thylakoid membranes.
(Refer to Figure 3)
(Refer to Figure 5)
__________________________________________________________
ü Excited electrons from Photo System I may be transferred to a carrier molecule and used to reduce
_____________
NADP+
ü This forms NADP- which is needed in (conjunction with ATP) for the light independent reactions
_____________________________________________________________
ü The electrons lost from Photosystem I are replaced by de-energized electrons from Photosystem
__________
II
_____________________________________________________________
ü The electrons lost from Photosystem II are replaced by electrons released from water via
__________
photolysis
ü Water is split by light energy into H+ ions (used in chemiosmosis) and oxygen (released as a by-
product)
The products of the light dependent reactions (ATP and NADPH) are used in the light independent reaction
(CALVIN CYCLE).
Figure7: Light Reactions: Light-dependent reactions harness energy from the sun to produce chemical bonds, ATP, and
NADPH. These energy-carrying molecules are made in the stroma where the Calvin cycle takes place. The Calvin cycle
is not totally independent of light since it relies on ATP and NADH, which are products of the light-dependent reactions.
Other names for light-independent reactions include the Calvin cycle, the Calvin-Benson cycle, and dark reactions. The
most outdated name is dark reactions, which can be misleading because it implies incorrectly that the reaction only
occurs at night or is independent of light, which is why most scientists and instructors no longer use it.
The light-independent reactions of the Calvin cycle can be organized into three basic stages: fixation, reduction, and
regeneration.
Stage 1: Fixation
In the stroma, in addition to CO2, two other components are present to initiate the light-independent reactions:
an enzyme called ribulose bisphosphate carboxylase (RuBisCO) and three molecules of ribulose
bisphosphate (RuBP). RuBP has five atoms of carbon, flanked by two phosphates. RuBisCO catalyzes a
reaction between CO2 and RuBP. For each CO2 molecule that reacts with one RuBP, two molecules of 3-
phosphoglyceric acid (3-PGA) form. 3-PGA has three carbons and one phosphate. Each turn of the cycle
involves only one RuBP and one carbon dioxide and forms two molecules of 3-PGA. The number of carbon
atoms remains the same, as the atoms move to form new bonds during the reactions (3 atoms from 3CO2 +
15 atoms from 3RuBP = 18 atoms in 3 atoms of 3-PGA). This process is called carbon fixation because CO2
is “fixed” from an inorganic form into organic molecules.
Stage 2: Reduction
ATP and NADPH are used to convert the six molecules of 3-PGA into six molecules of a chemical called
glyceraldehyde 3-phosphate (G3P). This is a reduction reaction because it involves the gain of electrons by
3-PGA. Recall that a reduction is the gain of an electron by an atom or molecule. Six molecules of both ATP
and NADPH are used. For ATP, energy is released with the loss of the terminal phosphate atom, converting
it to ADP; for NADPH, both energy and a hydrogen atom are lost, converting it into NADP+. Both of these
molecules return to the nearby light-dependent reactions to be reused and reenergized.
Stage 3: Regeneration
At this point, only one of the G3P molecules leaves the Calvin cycle and is sent to the cytoplasm to contribute
to the formation of other compounds needed by the plant. Because the G3P exported from the chloroplast
has three carbon atoms, it takes three “turns” of the Calvin cycle to fix enough net carbon to export one G3P.
But each turn makes two G3Ps, thus three turns make six G3Ps. One is exported while the remaining five
G3P molecules remain in the cycle and are used to regenerate RuBP, which enables the system to prepare
for more CO2 to be fixed. Three more molecules of ATP are used in these regeneration reactions.
Score:
ACTIVITY 4: it pays to have a label.
_____
Label the illustrations below with its descriptive terms. Choose from the boxes (WORD
BANK) provided before each illustration.
Score:
ACTIVITY 5: THINKING ABOUT YOUR OWN THINKING
_____
Directions: Understand the illustration below and supply answers to the questions indicated.
Go through the details of the illustration to suffice a concise understanding of the matter.
Come on! Think about your own thinking!
5. As shown in the figure above, what raw materials in photosynthesis were used in the light
dependent reaction and Calvin cycle?
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______________________________________________________________________________
______________________________________________________________________________
6. What is the relationship between the two specified reactions in the production of ATP’s and
NADPH?
______________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________
7.If light dependent reaction will not occur, do you think there’ll be Calvin Cycle? Justify.
______________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________
Score:
ACTIVITY 6: two in one _____
WORD BANK
1 2
3
4
5
6
9
10
11 12
14 15
16
17 18
13 7 19
20
ACROSS DOWN
1. The opening in a leaf that allow the passing 3. A living organism able to manufacture its
of gases into and out of the leaf. own food
2. An important gas produced in 5. Process in which the plants use the sun’s
photosynthesis energy to make food
4.A small membranous sac within a chloroplast 7. Organism that makes its own food from the
of a plant energy coming from the sun
6. Singular form of grana 8. Site of photosynthesis in plants
10.Needed for photosynthesis, along with 9. Sugar as end product of photosynthesis
carbon dioxide and sun
14.A nucleotide produced by the light 11. Water-based fluid filled area of the
dependent reaction chloroplast
16. Green pigment in plants 12.Protein molecules that aid in the
photosynthetic process
17.Stacks of thylakoid embedded in the stroma 13. Sugar formed from carbon dioxide and
of the chloroplast water
18. Original source of energy in the form of 15.Dark or light independent reaction
photons
19. Decomposition of molecules by the action
of light
20. A molecule needed as raw material for
photosynthesis
What Can I Do
Directions: Evaluate the analogy presented and answer the questions on the next page relative to
the concepts of light dependent reaction, and align it to how work in the analogy is done.
1 2 3
4 5 6
7 8
10 11
9
12
13
1. If given a chance to be assigned as the supervision head of the work indicated by the illustrations
in the previous page, what work ethics will you implement to motivate workers/employees to be
productive at work such that light independent reaction was also able to produce ATP and NADPH?
Specify at least 3 work ethics.
a. ____________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________
b. ____________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________
c. ____________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________
2. Indicate what term/step in the light dependent reaction is illustrated by the parts of the work
analogy. Choose from the terms and phrases provided.
Assessment
Directions: Encircle the letter of your chosen answer.
2. During the Calvin cycle, carbon is reduced, transforming carbon dioxide into simple sugars.
What is the main purpose of the light reactions?
A. To provide the energy and electrons for the Calvin Cycle
B. To capture energy and make sugar
C. To reflect green light
D. To make sugars
14. A mutation occurs in the chloroplasts of a lettuce plant, causing its thylakoid membranes to become
more permeable to charged ions. How might this mutation affect the Calvin cycle?
A. Fewer carbohydrates would be produced by the Calvin cycle.
B. There would be no change to the Calvin cycle.
C. More carbohydrates would be produced by the Calvin cycle.
D. Production of carbohydrates would be increased.
15. Which of the following correctly describes the overall process of the Calvin cycle?
A. The Calvin cycle occurs in the thylakoid membrane of chloroplasts.
B.ATP and NADPH produced by the light reactions are used by the Calvin cycle to make carbohydrates.
C. The Calvin cycle breaks down carbohydrates into simpler molecules.
D. During the Calvin cycle, carbon is oxidized.
Answer Key
ACTIVITY 1: RECALL:
1. F 4. B
2. E 5. D
3. C 6. A
ACTIVITY 3: Looking Through the PICS.
1. What enables electron to get excited and what happens when they are energized?
Photons from sunlight causes electron to become excited. The energy given to the electron allows it to break free
from an atom of the chlorophyll molecule and is transferred to a nearby primary electron acceptor.
2. Which photosystem is first hit by photons allowing reaction flow? Why does it start there?
Photosystem II (P680) is hit first because it is a good electron donor and can transfer its excited electron to a
primary electron acceptor, pheophytin. The electron will be passed on through the first leg of the photosynthetic
electron transport chain in a series of redox, or electron transfer reactions.
3. Of what importance does the electron transport chain plays in the light dependent reaction?
ETC helps establish a proton gradient that powers ATP production and also stores energy in the reduced
coenzyme NADPH which is used to power Calvin Cycle to produce sugar and carbohydrates.
4. What does water molecule pays in the light dependent reaction specifically in the production ATP and
NADPH?
Photolysis-splitting of water molecule gives way for a release of oxygen gas in plants and creation of a proton
gradient that helps in the production of ATP’s and NADPH.
ACTIVITY 6: puzzled!
ACROSS DOWN Analogy Relation to Light
1. stomata 3. plants Illustration Dependent
2. oxygen 5. photosynthesis Reaction
4. thylakoid 7. autotroph Sunlight photon
6. granum 8. chloroplast
Bridge with ship Electron transport
10. water 9. carbohydrate
wheel chain
14. ATP 11. stroma
16. chlorophyll 12. enzyme Trusses/Tower photosystems
17. grana 13.glucose Pail Electron acceptor
18. sun 15. Calvin Cycle Encircled letter E electron
19. photolysis
20. carbon dioxide
Multiple Choice:
1. C. To actively transport hydrogen ions from the stroma into the interior of the thylakoid
2. A. To provide the energy and electrons for the Calvin Cycle
3. A. Chemiosmosis
4. A. primary electron acceptor molecule
5. D. water split, oxygen and hydrogen ions released ATP formed
6. B. Reverse
7. A. Carbon Dioxide
8. B. Thylakoids
9. A. PGA
10. C. Chemiosmosis
11. C. The Calvin cycle requires ATP and NADPH, which require sunlight to be produced.
12. A. Carbon dioxide release
13. Molecule Reactant Product Both
CO2 x
ATP x
NADPH x
RuBP x
14. A. Fewer carbohydrates would be produced by the Calvin cycle.
15. B. ATP and NADPH produced by the light reactions are used by the Calvin cycle to make carbo
. REFERENCES:
https://www.gulfcoast.edu/current-students/academic-divisions/natural-sciences/biology-project/energy-
1/documents/fermentation-lesson-pre-post-test.pdf
https://www.ck12.org/book/ck-12-biology-concepts/section/2.31/
https://www.justledus.com
https://www. Hyperphysics
https://bit.ly/3itYrax.
https://bit.ly/3ixydEb
https://bit.ly/3jwBBzo
https://bit.ly/31K29Hj
https://www2.mcdaniel.edu/Biology/botf99/photo/p3igments.html
https://bit.ly/3gIcU0V
Editors: Margie Lou C. Jacob Adios, Patria Adorada, region del sol querida, Deja que el sol ardiento las lluvas evapore
Perla del Mar del Oriente, nuestro perdido Eden! Y al cielo tornen puras con mi clamor en pos,
Laarni A. Adonis Deja que un sér amigo mi fin temprano llore
A darte voy alegre la triste mustia vida,
Kathleen Joy B. Padilla Y fuera más fresca, más florida, Y en las serenas tardes cuando por mi alguien ore
Joly C. Baradero Tambien por tí la diera, la diera por tu bien. Ora Tambien, Oh Patria, por mi descanso á Dios!
En campos del batalla, luchando con delirio Ora por todos cuantos murieron sin ventura,
Reviewer: Sandy R. Albarico Por cuantos padecieron tormentos sin igual,
Otros te dan sus vidas sin dudas, sin pesar;
Illustrator: El sitio nada importa, ciprés, laurel ó lirio, Por nuestras pobres madres que gimen su amargura;
Layout Artist: Cadalso ó campo abierto, combate ó cruel martirio, Por huérfanos y viudas, por presos en tortura
Lo mismo es si lo piden la patria y el hogar. Y ora por tí que veas tu redencion final.
Management Team: Y cuando ya mi tumba de todos olvidada
Yo muero cuando veo que el cielo se colora
Y al fin anuncia el día trás lóbrego capuz; No tenga cruz ni Piedra que marquen su lugar,
Majarani M. Jacinto, Ed.D., CESO VI Si grana necesitas para teñir tu aurora, Deja que la are el hombre, la esparza con la azada,
SDS Vierte la sangre mía, derrámala en buen hora Y mis cenizas antes que vuelvan á la nada,
Y dórela un reflejo de su naciente luz. El polvo de tu alfombra que vayan á formar.
Visminda Q. Valde, Ed.D. Mis sueños cuando apenas muchacho adolescente, Entonces nada importa me pongas en olvido,
ASDS Mis sueños cuando joven ya IIeno de vigor, Tu atmósfera, tu espacio, tus valles cruzaré,
Feuron el verte un día, joya del mar de oriente Vibrante y limpia nota seré para tu oido,
Secos los negros ojos, alta la tersa frente, Aroma, luz, colores, rumor, canto, gemido
Raymond M. Salvador, Ed.D. Sin ceño, sin arrugas, sin manchas de rubor. Constante repitiendo la esencia de mi fé.
ASDS
Ensueño de mi vida, mi ardiente vivo anhelo, Mi Patria idolatrada, dolor de mis dolores,
Juliet A. Magallanes, Ed.D. Salud te grita el alma que pronto va á partir! Querida Filipinas, oye el postrer adios.
Salud! ah que es hermoso caer por darte vuelo, Ahi te dejo todo, mis padres, mis amores.
CID Chief Voy donde no hay esclavos, verdugos ni opresores.
Morir por darte vida, morir bajo tu cielo,
Y en tu encantada tierra la eternidad dormir. Donde la fé no mata, donde el que reyna es Dios
Florencio R. Caballero, DTE Si sobre mi sepulcro vieres brotar un dia Adios, padres y hermanos, trozos del alma mía,
Entre la espesa yerba sencilla, humilde flor, Amigos de la infancia en el perdido hogar,
EPS - LRMDS Dad gracias que descanso del fatigoso día;
Acércala a tus labios y besa al alma mía,
Y sienta yo en mi frente bajo la tumba fría Adios, dulce extrangera, mi amiga, mi alegria,
Sandy R. Albarico De tu ternura el soplo, de tu hálito el calor. Adios, queridos séres morir es descansar.
EPS - Science
Deja á la luna verme con luz tranquila y suave;
Deja que el alba envíe su resplandor fugaz,
Deja gemir al viento con su murmullo grave,
Y si desciende y posa sobre mi cruz un ave
Deja que el ave entone su cantino de paz.
ACTIVIT ACTIVITY 2 ACTIVITY 3
Y1 Photosynthesis Cellular 1. A
1. A Respiration 2. C
2. C • Chlorophyll • Mitochondria 3. B
3. B • Chloroplast • Animal cells
4. F • Plant cell • Produces
5. E • Produces O2 CO2
6. D • 6CO2+ • Glucose +
oxygen
Guide Question: