Optika ISview

Image acquisition and processing software

Instruction Manual
 Key to the Instruction Manual
IS is shortened name used for OptikaISview

Square brackets are used to indicate items such as menu

names, button names and window names that appear on the

computer screen.

>> indicates the selection procedure from the menu.

Example: [Capture] >> [Main Control] >> [FlatFielding]

: Marks tips for using the software.

: Marks information that should be read before use.

: Marks additional useful information.

 Help
Refer to [Help] >> [About OptikaISview] menu for software

information and technical support.

Provide the following information when it is required to obtain the

technical support:

1 Camera model and S/N (serial number);

2 Software version number;

3 Description of the problem. Screenshots of the problem would

be useful.
MAIN FEATURES OF IS........................................................................................................................4

SYSTEM REQUIREMENTS....................................................................................................................4

CHAPTER1: GETTING STARTED...........................................................................................................5

CHAPTER2: IMAGE ACQUISITION.....................................................................................................11

CHAPTER3: IMAGE MANAGEMENT..................................................................................................56

CHAPTER4: IMAGE PROCESSING.......................................................................................................58

Main Features of IS

Advanced camera control

Acquisition of still images and video. Available image types: JPEG,

BMP, TIFF and RAW.

Fluorescence imaging settings

Live image measurements

Image management

Extend depth of focus (Focus Stacking)

Image Stitching

Fluorescence combination function for still images

High dynamic range (HDR) function

Still image measurements

System requirements

OS Windows XP/ Vista/ 7/ 8 (32 & 64bit)

CPU Intel processor (Core2 Duo or higher is recommended)
Memory 2GB or More is recommended
USB ports USB2.0 Hi-Speed port
Chapter1: Getting Started

This chapter explains preparatory steps and basic OptikaISview

(hereinafter, “IS”) operations.

What You Need

To use the OPTIKA camera, you need to install the camera driver and

OptikaISview application software:

1. Find the OptikaISview Setup.exe file from the CD, double-click on it to

start the installation and hit “Next”.

2. The installer package selects the “C:\Program Files\” as the default

file destination.

3. After the installation is finished, the IS shortcut will be created on

the desktop.

Driver installation:

1. Double-click on the driver “Optika Camera Driver Setup.exe” to start

 the installation.

2. Follow the steps to finish the installation.

4. After the installation is finished, please go to the Device Manager to

check if the driver was installed properly. When the driver is installed

correctly, there is no yellow mark with the camera under the Imaging

Device in Device Manager. Please see the picture below:

[Capture] window-typical appearance
[Browse] window-typical appearance
[Image] window-typical appearance
Chapter2: Image Acquisition

Adjust camera parameter settings to get proper live image; live image

measurement and save still pictures and videos.

.

Start IS with the camera attached to a PC, the live image begins

automatically. If IS is run first before the camera is connected, click to

start the preview.

IS supports multiple camera functions (Only for H series or later). Stopping the

current preview enables the selection of cameras connected to the

system via the dropdown menu.

Basic Control

Provide basic camera settings:

After setting the brightness live images, it is recommended to apply

White Balance to correct the live image color. To get better white

balance effects, please follow the following steps:

1. Move the sample to the blank area;

2. Unselect [Color Enhancement]

(It is unselected by default in [Color Control] panel);

3. Push [White Balance];

4. Move the sample back.

Or you can follow the [WB Wizard] to get better white balance result.
Taking Still Images and Videos

In the [File format] dropdown menu , 4 file formats are available:

JPEG, BMP, TIFF and RAW.

Raw image file contains minimally processed data from the camera. It

needs to be read in imaging software, for example, Photoshop, ImagJ

etc. If the image is in the color camera raw file format, color information

only can be seen after decoding the Bayer matrix

In [Use File Save Dialog] mode , a pop up file save configure window

will appear when the [Capture] or [Video] button is pushed. Enter

the desired file name and directory path at this pop-up window.
 [Use File Save Config] , allows the file save name to be pre-set,

format, image quantity, capturing interval time and the file save

directory. Upon executing [Capture] or [Video] , IS will save files as

set.

Capturing and Saving Individual Images

Enter preferred name in the [File Name] field . If do not key in

anything, “IS” is used by default.

Select [Use Time-stamped] to name the image by the capture time

automatically. The time-stamp file name will be in the form of

“MMDDHHmmSS”. Here “MM” indicates the month; “DD” indicates

the day; “HH” indicates the hour; “mm” indicates the minutes; and

“SS” indicates the seconds.

Click [Capture] to take one image with the pre-set file name.
Capturing and Saving a set of Images

Click [Continuous Shooting] checkbox , the software will

automatically save a set of images after a single [Capture] is

executed.

Click [Config] to set continuous capturing image numbers and the

interval time .

This set of image file names follows the same format as set for

individual image capturing. If [File Name] is used, image names will

be in the form “X”, “X-1”, “X-2” (where X is the character/s you

entered or “IS” by default).

Video recording

Click [Video] / , start/ stop video recording.

Click [Rec Config] to get video recording configure window.

It provides [Manual] and [Auto] modes to stop the recording.

In [Manual] mode, you need to click on [Video] button to start and stop

the recording.

In [Auto] mode, pre-set the number of frames or the time for videos and

click on [Video], IS will stop the recording automatically after pre-set

 number of frames are saved or pre-set time is up.

[Rec Config]>>[Codec] will also list all the available video

compressors in PC.

The video taken without compression will be very large size. IS

will automatically search the installed video compressors on the PC.

File Save Destination

Click [Browse...] to change file save destination. The default path is the

software installation folder. Usually it is “C:\Program

Files\OptikaISview\”.
This default destination might NOT be allowed storing files if the user is

not logged in as Administrator in Windows Vista, 7 or later. Image may not save

if the default path is used. We recommend either to change the file

saving path or release this folder “Write” authority for other user

accounts.

Exposure Control

Change the Exposure time and Gain to adjust the image brightness.
Select frame speed to get different live image frame rate. Set 8-bit or 16-

bit data width for captured images.

Auto Exposure

Check [Auto Exposure] checkbox, software start to adjust the

exposure time automatically to get proper brightness of live images.

Auto exposure target value: Set the reference exposure time for auto

exposure adjustment. It helps the auto exposure to find the proper

exposure time faster. For example, if the imaging target is quite

bright, a lower target value is set it will indicate a shorter exposure

time to the software.

Lock: will stop the auto exposure calculation. While auto exposure is working,

it will keep on calculating the image brightness to get proper

exposure time. During this, if you already see a good live image for

you, you can push to lock it.

Manual Exposure

Adjust the exposure time manually. Two ways to change the exposure

time:

Key in the exposure time in the edit box directly , then click to

confirm it.

Pull the slide bar to change the exposure time.

[Extend] is used to get longer exposure time. This function is ONLY

available for CCD cameras. For other cameras especially the CMOS
camera, the maximum exposure time is shorter than 1 second, then

[Extend] will be gray out.

[Update] appears after [Extend] is selected. Click on to stop the

previous exposure time and restart the new one immediately. For long exposure

applications, we strongly recommend that [Update] is used to start a

new setting. This will help to get the new exposed image earlier. If the

exposure time is less than 2-3 seconds, it is not necessary to use it.
Gain, Frame Speed & Data Width
Color Control

Is used for adjusting image color, gamma, contrast and saturations.

Flat Fielding Function

Flat fielding function is used to correct the uneven background brightness.

Click on [FlatFielding] to start the flat fielding parameter calculation

and apply it to the live images.

 When the check boxis unchecked, the calculated flat fielding

parameter is NOT applied to the live images.

To get better flat fielding result, Move the sample to a blank area first,

apply the flat fielding, then move back the sample.

When the lighting is changed, re-do the [FlatFielding] to correct the uneven

brightness or when using a different objective.

Fluorescence Settings

Included in our software are useful parameter settings for fluorescence

or low light imaging. It helps to get better images easier and faster.

Black Level

Black level function defines the brightness level at the darkest part of the

image. In low light imaging, it can help to see more details in the dark

area.
In low light application, it usually needs quite a long exposure time to get

proper images. If you set a long exposure time at the beginning, you

might need quite a long time to find your target and get a proper image

(wait for finishing a long exposure to get a new frame image, adjust,

wait...). When searching for the imaging target at the beginning, we

recommend to set a shorter exposure time, but make larger Gain and Black level first .

After you find the target, then reduce the Gain and Black level, and

increase the exposure time. This will aid in a better image acquisition.
Levels

Live image histogram.

To adjust the live image levels automatically: select [Auto] check box.

Adjust image levels manually:

Pull the little triangles to adjust the live image levels. Move the white

triangle towards left, it is able to reveal some information in dark area. If

move the black triangle towards right, it will reveal bright area information.

You can also key in the image levels directly

After adjusting the levels, click to confirm the setting. If you need to go

back to the original image, click to restore the image.

Capture Mode

Three capture modes are specially developed for fluorescence imaging.

Parameter Group

Save parameter sets for different applications. The saved parameters

include exposure time, gain, frame speed, data width, gamma, contrast,

saturation, color enhancement status, monochrome, RGB gain and black

level. It allows users to save 20 set parameters (available in Ver 3.6 or later).

Save parameter function: Enter a name for current parameter settings, click

to save it.

Load parameter function: Click to open drop-down menu, click on

preferred parameter name and then push to make selected

parameters take effect on the live image.

Live Image Measurement

Click on [Measure] at the top of the IS to get the measurement tools

Show Scale Line On/off the scale line on the picture

Calibrate Create Calibration file

Calibrate Table Available calibration file list. Allow to add, edit and delete
calibration file.

Decimal Set measurement precision. Allowed decimal range is from 0

to 7

Measurement List List all the measurement data

Layer Create multiple layers to apply measurements and save layer

information
 Delete All Delete all the measurements and layers

UnLock/Lock Unlock/lock the measurement operation. Allow to do same

measurement continually when LOCKED.
/ It is locked by DEFAULT.
Select Select to change measurement or the measurement data
position

Line Measure the length

Parallel Measure the distance of parallel. Allow to do multiple

parallels’ distance measurement. Double clicking to end
parallel measurement.
Perpendicular Measure the perpendicular length. Allow to do multiple
perpendiculars’ length measurement. Double clicking to end
perpendicular measurement.
Rectangle Measure rectangle height, width, area and perimeter.

2-points Circle Use center point and point on the circle to draw a circle. Give
the radius, area and perimeter of circle

3-points Circle Use 3 points on the circle to draw a circle. Give the radius,
area and perimeter of circle

Diameter Circle Draw a circle according to the diameter. Give the radius, area
and perimeter of circle

Concentric Circle Use center point and radius to draw concentric circles. Give
concentric circles’ radius, area and perimeter. Allow to do
multiple concentric circles measurement. Double clicking to
end concentric circles measurement
Polygon Measure polygon area and perimeter.

Arc Measure a curve angle, radius and length.

Angle Measure the angle

Point Counter. Count the quantity.

Annotate Add remarks on the images.

 Delete Delete previous measurement. Select it then click on the
measurement to delete the measurement.

Cross-ruler On or off cross-ruler on the images. The unit of the ruler

depends on the applied calibration file.
Edit Scale Line

Double click on the scale to get its properties and make changes to it.

Edit scale character

Edit the frame of the scale

Edit scale line length and name

Create Calibration File

To measure the samples real size, the corresponding calibration file

needs to be created first.

1. Take pictures of the calibration slide in all the required working

objectives and resolution (if a reducing lens is also used in your application, it also

requires you to take the calibration slide picture with the reduce lens attached).

If ONLY ONE objective and ONE resolution is used in the application, one

calibration slide picture is enough. The calibration slide picture MUST be

taken with exactly the same lens or microscope settings as the target

image taken.

2. Click to start to create calibration file.

Using longer length as the reference length will give more accurate

measurement results. For example, using 10 scale units as reference

length will give more accurate result than using 1 scale unit.
If you need more than one calibration file, using objective+reducing lens(if it is

used)+resolution as the name of the calibration file is recommended. This

can help to prevent using the wrong file to do the calibration.

When keying in the length, please pay more attention to the calibration

scale unit and the Measure Unit used here. For example, the calibration scale

unit is 0.1mm; the Measure Unit is selected as µm; and the reference

length is 10 scale units, so the length should be 10 x 0.1mm x 1000 =

1000 µm.

6. Click [OK] to confirm the calibration. The new calibration file named

“10X” is created in the [Calibrate Table].

Calibration Table

Click [Calibrate Table] to open the calibration table.

Select the correct calibration file for current image measurement.

Using the WRONG calibration file will make the measurement result

innacurate. Please make sure the calibration file is correctly corresponding

to the current image. Hence, it is useful to name the calibration file with

the capturing settings or objective name.

Measurement List

All the measurement data is listed in the [Measurement List]. The

software allows you to export all the measurement data to TXT or Excel file.
Measurement

IS allows you to do line, parallel, perpendicular, rectangle, circle,

polygon, arc and angle measurement. The [Count] function allows you to

manually count the objects. And the [Annotate] function offers to add

comments on the images.

Double click on the measure data to get the measurement configure

window. It allows you to change the measured data name, color,

thickness, background color and the character font.

Annotate

Select [Annotate] and click on the image area which you prefer to add a

remark. It allows you to edit the comment, change the background color

and on/off the annotate arrow.

Ruler

Click on to show or cancel the cross-line on the images. The displayed

ruler unit is determined by the selected calibration file. Double-click on the
ruler to get the ruler property and change the ruler color. The default color is
BLACK.
Layer

Create multiple layers for loads of measurements. The layer function

makes adding a large number of measurements on the processed image

review simple and easy.

If you have already applied some measurements on the image, the

[Layer] function automatically creates “Background” and “Layer01” for

the current image.

Click [New] to create a new layer. Allow to key in the preferred name for
the new layer. It uses “Layer02”, “Layer03”... etc as the layer name by

default.

Now loads of measurements can be applied on different layers. It allows

you to choose any layers to view.

Checked [Cur] means the corresponding layer is displayed currently.

Select different [Cur] to switch between different layers. In the [Visible]

column, the selected check box means all the measurements in the

corresponding layers also display on the current layer. Uncheck the check

box, and the corresponding measurement will be invisible in the current

layer
The layer information is saved in a text file.

Click [Browse] to choose the text file saving directory and enter file

name. Then click [Save] to save the current layer information in the

text file. The layer information will be saved as “LayerInfo.txt” in Disk D by default

Click [Browse] to find the existed layer information text file. Click
 [Load] to load the layer information to the current image.

Live image shortcut

On the right hand side of the live image window, some shortcuts are

provided to process the live image quickly.

Chapter3: Image management

View images in [Browse] panel, it displays the image File name, capturing

time, color depth (bit), picture resolution and image size. It also allows

you to add comments to any individual image. When you view this image next

time in the IS, it will show the image comment.

Image Management and Processing Shortcuts
IS provides some quick functions on the right hand side of the software

in Browse or Image mode.

Chapter4: Image Processing

In this section, IS provides advanced image processing functions and also

allows you to do the measurement on the still images.

Image Processing

Provide basic captured image processing functions and allows functions

such as extended Depth of Focus and image stitching.

After clicking [Apply], all the settings are applied to the image. Then you

can NOT revert to the original image.

Level

Push [Levels] to get the image histogram. It allows you to adjust the

image levels. The level adjustment is the same as live image level

adjustment. Get more detail in [Capture]-->> [Fluorescence].

Extend depth of focus

Push [Extend DoF] to get below dialog. Select the corresponding images

and apply the function.

Browse the image folder which you are going to do the stacking.

All the images in the folder will be listed on the left hand side. Click

on one image, the image will be hightlighted in BLUE.

Click [Add] to add the highlighted image to the right hand side (the

selected source images for stacking).

[Add all] button allows to add all the same size images in the left hand

side to the right as stacking source images by just one click.

Click [Fusion] to stack all the selected source images and get an

image with an extended depth of field.

When selecting a wrong image as stacking source, just click on it and

then click [Delete] to remove it. [Delete all] will remove all the selected

images.

Image stitching

Click on to get the image stitching configuration. It combines multiple

images with overlapping fields of view to produce a large panorama or

high-resolution image.

1) Click [Open] browse the stitching source images. Select all the source

images and open them.

2) Click [Stitching] to start stitching all the source images.

3) Click [Save] to save the stitched image in the same directory as the

source images with the name of date and time stamped.

Select [Use GPU] checkbox to speed up the stitching progress. This function

only works if the computer is equipped with GPU.

Sample images :
Measure

Click on [Measure] at the top of the IS to get the measurement tools.

Then select the corresponding tools to measure the still images (get

more details in [Capture]>>[Live Measurement]).

Label

The label text will be displayed on the lower right corner of the image.

The date and time will be displayed on the top right corner of the image.

After clicking [Save], the image with the label will be saved as image

file name+ _bak. For example, the original image file name is “IS.jpg”,
 then this image with a label will be saved as “IS_bak.jpg”. So the

original image is still kept.

HDR Image

High Dynamic Range (HDR) image is used to get greater dynamic range of

an image.

Take pictures for one same scene with different exposure time and load

them in the software.

In the drop-down menu, select corresponding images for [Exposure

Low], [Exposure High] and [Exposure Suitable].

Push [HDR] button to combine different exposed images into one.

The generated HDR image will be named as “hdr_image”.

If the different exposed images are not loaded in the OptikaISview yet,

the shortcut on the right hand side of the OptikaISview allows you to

browse any image simply.

Fluorescence

This function is used to assign fluorescence images with different colors

and combine them together into one image.

Step 1: Open the images which are used for combination in IS, then click

on [Start] to start the fluorescence combination.

Step 2: Click on image thumbnails to add corresponding images. The

image position indicator shows the added images’ position. Maximum 4

frame images are allowed to add for fluorescence combination.

Step 3: Click on one added image indicator to start applying color for it.

Click on one image indicator to select it (The selected one will be in dark

color, unselected ones will be gray white).

Assign color for selected image.

Two ways provided for color assignment:

a. Click on the preferred color or slide to choose it.

b. Assign the color according to the dyestuff in the drop-

down menu of [Dye Type].

 Click on [Apply Color] button to add selected color on the

image.
Step 4: Click on [Apply All] to combine all the colored images.

Optimize checkbox is recommended to select during the combination. It

will optimize image background to get a better image. But without

optimization, the created image keep all the original information. No

extra processing is applied to the image data.

After get the combined fluorescence image,

[Sharp] function in [Image Processing] can help to get sharper images and see

more image details.

If add wrong image or wrong color to selected image, just click on the

tiny cross beside each indicator to delete it. If want to cancel current

combination, just click on [Close All] to cancel the combination.

Original images:
Combined image: