Genetics Exam 1-210 NAME: Problems are 4 points uress otherwise stated ‘Multiple-choice questions may have more than one answer. Circle ALL appropriate answers. 4, How many different types of gametes can an individual with the genotype AaBiccDDEeFF form? oe ® 3 Soe A 73 ae eae ce 2, Mende’ law of independent assortment has its physical basis inthe: a, sister chromatids separating in meiosis i (2 random arangement of chromosomes on the metaphase plate in meiosis & formation > chiasmata d. separation of homologous chromosomes 3. Homologous chromosomes: (contain the same genes mina b._sreldeticin DNA sequence cnesse wong @)—_dgnat mataghace to frm tetrad cnwer er “Ge egegnteetmetod dont cheese correct 4, Complementation tests are used to determine: 2. map poston b.—_episass G) two mutations are ales of the some gene 4. nondisjunction ocurred 5, Marfan Syndrome isa rare autosomal dominant disease that affects connective tissue and affects the heart, eyes, blood vesselsand skeleton, A man with Marfan Syndrome (assume heterozygosity) marries a normal woman, The woman's father was a hemophilia (rare X-linked recessive). What is the probability that their ist son will be a hemophiliac with Marfan Syndrome? Afdue @ fe do showed c ae worl « y ‘ “A ie doe “Ons tt auc6. (@pts) In the pedigree below individual I-2 has a disease that is inherited in an autosomal recessive pattern. Ais the dominant allele, and ais the recessive allele, the genotype of 1:2 is: a AA @ 4 4. insufficient data 12 1 0 faeces! The genotype of -1 is a AA ba @)_insufclen: data The probability that isheterozygous Aa is Pane @ ow cae 48 IFparents It and 12 had another child, what isthe probability oft inheriting the disease? @m b 28 «12 a7.(6 pts) (© Dominant pense) Sia iat sueaigeea aig ‘This gel shows an analysisofa RFLP locus below each person ina pedigree of a dominant disease allele P. Which molecular bandls) appear linked to P? M-R Which bands) appear tobe allelic to thern? K How many alleles ofthis RFLP locus appear tobe represented inthis family? a ee | 8, (4 pts) Consider a rat ofthe following genotype: ae aa . a. If this animal is testcrossed, what proportion of progeny will be AaBbcc? - Sto get An Bh ce tad Se ee Wy ome of tha tee C0. produets4 8, (4pts) The following cioss is made in Neurospora: a+ bt ct d+ x ab ¢ d (loci are linked In the order written, centromere lies before a). Construct tetrad diagrams to illustrate how the ordered ascl patterns below could arise during Meiosis Draw the tetrad with centromeres and Indicate where the crossovers occur. abet rei abed teed tbed aber 410, (4 pts) The diagrams below show anephases in individval cells ofa diploid organiem of genotype ‘A/o; B/o, where genes A nd 8 are on different chromosomes. State whether each drawing represents ‘mitosis, meiosis |, melosistI, or an impossible situation for this particular genotype. WNYN aa x ¢ ans Na a Me mvtess — =1 ve 11, (6 pts) Ina population of rabbits, you ifnd three different coat colors: chinchilla (C), Himalaya (H), and albino (A). To understand the inheritance of coat colors, you cross ndividual rabbits with each other and note the results: ‘rossnumber___ parental phenotypes progeny phenotypes + HEH WA HA AHEMA 2 HA Head MHRA a exe CMa ker 4 cen CCHS atc 5 cxe th A MCLKA 6 fea HSH am 7 CMA CAAA werHA 8 AKA AAS AA alla 9 cxH CH FH KCKH v 10 exh UM «HA WCrMHIMA What stheinertance pattern of coat colon rabis? Sine gone jwullde allies C >HOA LUst genotypes of parents for crosses 1,2, 9,10 (write next to the cross above). \What kinds of progeny would you expect and in what proportions if you crossed the chinchilla parents in erozzes 9 and 10? Va chowclatle mb} benalaye12, (6 pts). You have come into contact with two unrelated patients who have a very rare ‘condition -a dark spot onthe bottom of the foot. Two patients give their family histories and ‘you generate the pedigrees below. Assuming that people marrying into the family do not carry ‘the mutant allele, iit dominant or recessive, and what do you conclude about it Smith Indviduals 1-3 and il-6, and Jefferson 1-6? ie phenctyp-e ithe rate was instead qute commen what stermative explanation would you propose forthe inhertance pattern? Whit would be the genotypes of Smith I'd andra? What would be the fenotypes of lefferson 2, r6 and 7? autosomal recessive al beberezyqcus “te Shs +6 6 bo PHS RE : bene 1 970 2 3146576) » Ge 65md7 13, (6 pts) In Drosophio, mutants 1 through 9 all have the same phenotype: the absence of red pigment in the eyes. in pair-wise combinations in complementation tests the following results \were produced, where + equals complementation and ~ equals no complementation. How ‘many genes are there an which mutants have defects in the same gene? [oo soe of oe ee at GS 44 See 26 oS 3, 6.4 Soe 3,7 Yo Ganso £14, (20 pts) For each ofthe following definitions, indicate which ofthe following genetic principles 1-5 is at work (put number from below to the left of the definition on the line. =: 2 os LF Beetles can have blue, green, or turquoise wing covers. Inter crosses of these beetles give 3:1, 14, oF 1:2:1 ratios in the progeny. With regard to the ABO blood group, people can be AB, A, Bor 0. The RBCs of AB people have both A and 8 cel surface antigens. refers to agene present in only one copy in a diploid organism, far example, one found on the X chromosome in a male. fallure of tomolog separation in meiosis ‘An individual carrying mutations in two different genes dies, even though individuals carrying ether mutation survives alone. Synthetic lethality Codominance ‘Multiple aletes Nondisjunction hemizygous8 415, (8 pts) The data below show 1000 gametes observed from a trihybrd parent in @3-pointtestcross to determine the geneticmap of the 3 linked genes. DeF 390 45 det 390 Def 60 dEF 60 Der 40 der 40 det 6 Der _@ Total: 1000 ‘Whats the genotype ofthe trihybrid parent (deduce from parental)? vee DeF /AEL Wich are the double crossover progeny? ae def , DEF ‘What is the map distance between the genes (show all three pairwise recombination frequencies and how youdeal with any discrepancies)? Draw the map with distances. D-E > bo rbsstet five > ite fogs = Be Bau > Yo r4oreru 3ps a bri ftece Fohooe = 404 ee Vmu Bop = BO hee nite T= sales = 3 poe > «3 €4F Fe dceset add up right Wwecwus dott cout deo se tp Fb WO YO MON Gry #Gr4 : ‘ = Ras/, foe [reac So - 5 = = eA or DD me416. (10 pts) Suppose youhave a plant with petals that are normally blue. Suddenly you see some white-petaled plants. You cross the white with the blue and you get an all F: population that isa blue. In the Fz generation you see a ratio of 3:1 blue to white-petaled plants. 1a} What does thisresult indicate? Draw the crosses listing genotypes. WW + we 1 Ww 4 a Wo 4 4 be 1s Wu «+ Nw — Se “Another pure line of flowers shows pink petals. You cross the pink with the blue and once again the Fs generation shows al plants with blue petals. In the , generation the ratio is 3:1 blue to pink-petaled plants. What does thisresult indicate? Draw the crosses. > « Pi | ~ VPP ks ble , a = a ° \ e ® Veg Fb pie Next, you cross the pink znd the white. You get all blue in the F; generation! In the generation you see a segregation of 9:4:3 blue to white to pink plants. ) What phenomenon does this ratio suggest, Draw the crosses and lst genotypes and phenotypes. WN ge x ew PP ek | ye \10 17, (8 pts) in each ofthe following cross schemes, two pure-breeding plant strains are crossed to make FA plants, all of which have purple flowers. The F1 plants are then selfed to produce F2 progeny as shown below: ross Parents FL 2 : 1 blue x white purple S purple: 4 white: 2 white x white purple purple: 7 white 3 red xblue purple purple: 3red: 3 blue: 1 white ‘2 How many genes are involved in each cross (there is only one answer)? Qi dbad « modGed Arkybrd rates b. For the first cross, show a possible biochemical pathway that could explain the data, color begs ——> yw & ae - oy «. Which of these crosses is compatible with an underiying biochemical pathway involving only a single step that fs catalyzed by an enayme with two dissimilar subunits, both of which are required for enzyme activity? oe a | o Colne ¢ ————<@_ “> pur 4d, For Cross 1, what would you expect inthe F2 generation i the relevant genes were tightly linked {assume no crossing over? be Mh 5. «6 Aw Al ii ir are) Oa x \ Ab/Ab blag As ¢ > Mead ~ purple