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    (2017 Scheme) : Instrumental Methods of Analysis

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    (2017 Scheme) : Instrumental Methods of Analysis

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    JASMIN T
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    721006 (4)

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    (2017 Scheme) : Instrumental Methods of Analysis

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    JASMIN T

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    of 1

    QP Code: 721006 Reg. No……………………….

    Seventh Semester B. Pharm Degree Regular/Supplementary


    Examinations February 2023
    Instrumental Methods of Analysis
    (2017 Scheme)
    Time: 3 Hours Max. Marks: 75
     Answer all questions to the point neatly and legibly • Do not leave any blank pages between
    answers • Indicate the question number correctly for the answer in the margin space
     Answer all parts of a single question together • Leave sufficient space between answers
     Draw diagrams wherever necessary
    Essays (2x10=20)
    1. With the help of a ray diagram, explain the instrumentation requirements of UV
    spectrophotometer. Discuss the working of Photomultiplier tube in detail.
    2. Define and classify Chromatography. With the help of van-Deemter equation and
    van-Deemter plot, discuss the factors influencing the efficiency of separation.
    Short Notes (7x5=35)
    3. Explain simultaneous equation method of multicomponent analysis.
    4. With the help of neat and labelled diagram, write a note on Golay detector used
    in IR spectroscopy.
    5. Explain the construction and working of Hollow Cathode Lamp with the
    help of a neat diagram.
    6. Discuss the steps involved in Paper chromatography. Discuss the importance of
    Chamber saturation in paper chromatograpy.
    7. Discuss the mechanism of ion exchange chromatography. Discuss the
    applications of ion exchange chromatography.
    8. Explain the construction and working of thermal conductivity detector in GC.
    9. What is the principle of Electrophoresis. Explain the factors in electrophoresis.
    Write the procedure and applications of paper electrophoresis.
    Answer Briefly (10x2=20)
    10. Differentiate between Bathochromic and hypsochromic shifts with the help of UV
    Spectra.
    11. What is Quenching. List the factors responsible for quenching of fluorescence.
    12. What are the ideal properties of a UV detector.
    13. Why emission wavelength is always longer than absorption wavelength.
    14. Write the formulae and explain how the Number of Theoretical plate can be
    determined from a chromatogram
    15. Why activation of chromatographic plate is important in adsorption TLC
    16. Explain the principle of affinity chromatography.

    17. Differentiate between Isocratic elution and gradient elution in HPLC.


    18. Write briefly on carrier gases used in GC.
    19. Explain the working of “Rotary sample valve/loop injection” used for sample
    injection in HPLC.
    *******************

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