I.

Course Learning Syllabus

Course Course Course L T P C
18BTC105J Molecular Biology C Professional Core
Code Name Category 3 0 2 4

Co-requisite Progressive
Pre-requisite Courses 18BTC104T Nil 18BTC201J
Courses Courses
Course Offering Department Biotechnology Data Book / Codes/Standards Nil

Course Learning Rationale

The purpose of learning this course is to: Learning Program Outcomes (PO)
(CLR):

PSO PSO
CLR-1 : Know the structures of nucleic acids. 1 1 2 3 4 5 6 7 8 9 10 11 12 PSO-1
-2 -3
CLR-2 : Apprehend the copying mechanism of hereditary materials.

Individual & Team Work

Engineering Knowledge
CLR-3 : Adopt the structure of nucleic acids for their expression.

Design & Development

Project Mgt. & Finance

CLR-4 : Explain the basis and mechanism of protein synthesis

hereditary materials
Modern Tool Usage

Gene expression at
Blooms level

Basic knowledge in

Regulation of gene
Life Long Learning
Problem Analysis

Society & Culture

Analysis, Design,
CLR-5 : Understand the structure and machinery of nucleic acids responsible for cell functioning.

Communication
Environment &

different levels
CLR-6: Scrutinize the regulation of gene expression under anabolic and catabolic conditions.

Sustainability

expression
Research

Ethics
Course Outcomes (CO): At the end of this course, learners will be able to:
CO-1 : Reminisce the structure of nucleic acids 3 - 3 - - - - - - - - - - - 3 3
CO-2 : Comprehend the analysis of nucleic acids at the DNA and RNA levels 2 - 3 3 - - - - - - - - - - 3 3
CO-3 : Relate the DNA expression at the different levels 4 - - 3 - - - - - - - - - - 3 3
CO-4 : Dissect the mechanisms of protein synthesis with the genetic code 4 3 - 3 - - - - - - - - - - 3 3
CO-5 : Assess the various regulatory elements controlling gene expression. 5 3 3 3 - - - - - - - - - - 3 3
CO-6 : Invoke the different gene regulatory mechanisms at the cellular level 3 3 3 3 - - - - - - - - - - 3 3

Duration (hour) 15 15 15 15 15
RNA polymerases in prokaryotic and
SLO-1 Scope and history Basic rules for replication Genetic code Gene regulation
eukaryotic cells
S-1
SLO-2 Proof for DNA as the genetic material Chemistry of DNA synthesis Types and function of RNA polymerases wobble hypothesis Principles of gene regulation
Structure and function of the promoters
SLO-1 Proof for semi conservative replication Semi discontinuous replication Translation in prokaryotic cells Transcriptional gene regulation
S-2
Fine structure of prokaryotic and eukaryotic
SLO-2 DNA constituents Pulse chase and pulse labeling experiment Initiation of translation Post transcriptional gene regulation
genes
Transcription of RNA in prokaryotes -
SLO-1 Nucleoside and Nucleotide Enzymes involved in replication Elongation of translation Activators
initiation
S-3
Types and functions of DNA polymerases
SLO-2 Structure of DNA Elongation and termination Translocation Co-activators
in prokaryotic and eukaryotic replication
S SLO-1 Lab 1: Isolation of genomic DNA from Lab 7: Polyacrylamide gel electrophoresis
Lab 4: Plasmid DNA isolation Lab 10: Repeat/Revision of experiments Lab 13: Ligation of digested DNA
4-5 SLO-2 bacteria of DNA
SLO-1 Base pairing and base stacking Proof reading activity Transcription in eukaryotes Termination of translation Suppressors – Co-suppressors
S-6
5’-3’ exonuclease activity and Structure of promoters in mRNA, rRNA,
SLO-2 Models of DNA Ribosome recycling Moderators, Silencers and Enhancers
Topoisomerase activity and tRNA genes
S-7 SLO-1 Double helix Events in the replication fork Transcription of mRNA Translation in eukaryotic cells Operons
 Steps in transcription by RNA
SLO-2 Features of Watson and crick model Telomeric DNA replication Polyribosome Positive and negative regulation
polymerase II
Models of DNA replication – Bidirectional Transcription of tRNA by RNA
SLO-1 Major and minor groove Post translational modifications Lac Operon
replication polymerase III
S-8
Transcription of rRNA by RNA
SLO-2 Forms of DNA - A, B, Z Plasmid replication-theta model Protein folding Regulation of Lac operon by glucose
polymerase I
S SLO-1 Lab 2: Qualitative analyses of genomic Lab 5: Qualitative analyses of plasmid Lab 11: Restriction digestion of Plasmid Lab 14: Effect of UV rays in the bacterial cell
Lab 8: Isolation of RNA
9-10 SLO-2 DNA DNA DNA growth
Structure and function of RNAs– mRNA,
SLO-1 Strand displacement model Processing of tRNA Protein sorting and targeting Trp Operon
rRNA and tRNA
S-11
SLO-2 Secondary structures in RNA Rolling circle model Processing of rRNA Types of Protein targeting Control of Trp operon by Attenuator
Post transcriptional processing of mRNAs Principles of protein sorting and targeting
SLO-1 DNA Topology Bidirectional replication Ara Operon
– 5’capping into mitochondria
S-12
Principles of protein sorting and targeting
SLO-2 Supercoiling – Twist - Writhe Unidirectional replication Polyadenylation Regulation of Ara operon
into endoplasmic reticulum
DNA repair: Nucleotide excision and Principles of protein sorting and targeting
SLO-1 Linking number Splicing (including different types) Gal Operon
Mismatch repair into nucleus
S-13
Photo-reactivation, Recombination repair Principles of protein sorting and targeting
SLO-2 Change in linking number Alternative splicing Regulation of Gal operon
and SOS repair into chloroplast
S SLO-1 Lab 3: Quantitative analyses of genomic Lab 6: Quantitative analyses of plasmid Lab 9: Qualitative and quantitative Lab 12: Restriction digestion of genomic
Lab 15: Polymerase Chain Reaction
14-15 SLO-2 DNA DNA analyses of RNA DNA
1. 3. Benjamin Lewin, Genes IX, Benjamin Cummings, 2007
Learning James D Watson, Molecular Biology of Gene, Pearson Education, 2017
2. 4. G.M. Malacinski, David Friefelder, Essentials of Molecular Biology, 4th ed., Narosa Publishers
Resources Robert Weaver, Molecular Biology, McGraw-Hill, 2011
2008

Learning Assessment
Bloom’s Continuous Learning Assessment (50% weightage)
Final Examination (50% weightage)
Level of CLA – 1 (10%) CLA – 2 (15%) CLA – 3 (15%) CLA – 4 (10%)#
Thinking Theory Practice Theory Practice Theory Practice Theory Practice Theory Practice
Level 1 Remember 10% 5% 10% 5% 10% 5% 5% 5% 10% 5%
Level 2 Understand 15% 10% 15% 10% 15% 10% 10% 5% 15% 10%

Level 3 Apply 15% 15% 15% 15% 15% 15% 15% 15% 15% 15%
Level 4 Analyze 10% 20% 10% 20% 10% 205 15% 10% 10% 20%

Level 5 Evaluate 5% 10%

Level 6 Create 5%
Total 100 % 100 % 100 % 100 % 100 %
# CLA – 4 can be from any combination of these: Assignments, Seminars, Tech Talks, Mini-Projects, Case-Studies, Self-Study, MOOCs, Certifications, Conf. Paper etc.,

Course Designers
Experts from Industry Experts from Higher Technical Institutions Internal Experts
1. Dr. S. Sam Gunasekar, Orchid Chemicals and Pharmaceuticals Ltd., [email protected] 1. Dr. A. Gnanamani, CSIR-Central Leather Research Institute, [email protected] 1. Dr.N. Selvamurugan, SRMIST
2. Dr. D. Gunaseelan, BIOCON Ltd., [email protected] 2. Dr. Anbumani Sadasivam, CSIR-Indian Institute of Toxicology Research,[email protected] 2. Dr. S.Barathi, SRMIST