Details of Curriculum for Second Year

Diploma in Lab Technician

PAPER 1st Topics Hours.

Theory
1. History taking. General examination of the patient. Filling 05 Hrs
Case-sheet. Common clinical words.

2. Hypertension:- Def, Causes, Pathology, Clinical fectures, 03 Hrs

Investigation & Management.

3. Hypotension :- Def, Causes, Pathology, Clinical fectures, 01 Hr

Investigation & Management.

4. Diabetes mellitus :- Def, Causes, Pathology, Clinical 05 Hrs

fectures, Investigation & Management.
1.Only
relevant
surgical & 5. Diseases of blood :- Anaemia, Basics of coagulation 20 Hrs
medical Bleeding disorders & Haemophilia.
conditions
(relevant to 6. Respiratory Tract :- Pneumonia, Tuberculosis, B.asthma, 15 Hrs
Lab COPD, Bronchiectasis, Collapse of lung, Pneumonitis,
technician). Pleural effusion, Pneumothorax, Empyema thoracis, Cancer
lung.

7. Diseases of GIT & Liver & GB :-Reflux Oesophagitis, 20 Hrs

Peptic ulecrs, Gastritis, Instestinal Obstruction, Hepatitis,
Cirrhosis of liver, Cholecystitis, appendicitis, Hernia, Piles,
Fissure, Fistula, Pancreatitis, Pancreatic Cancer.

8. Diseases of Nervous system:- Stroke, Meningo-encephalitis, 20 Hrs

Glasgow coma scale, Epilepsy, Head Injury.

9. Diseases of Urinary tract:- Urolithiasis, Benign prostatic 10 Hrs

hyperplasia, Hydrocoele, Cancer prostate, urethral stricture,
Hypo & epi-spadias.

10. Endocrine system :- Diabetes mellitus, hypo & Hyper 05 Hrs

thyroidism.

11. Miscellaneous:- Hypo & Hyper Natraemia, Hypo & Hyper 05 Hrs
Kalaemia, Hypo & Hyper Calcaemia.

12. Infections diseases :- TB, Typhoid, Malaria, Dengue fever, 15 Hrs

Leprosy, AIDS, Amoebiasis.

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 Details of Curriculum for Second Year
Diploma in Lab Technician

PAPER 1st Topics Hours.

Theory
13. Head injury & Intracranial bleed. 10 Hrs

1.Only 14. D’s of G& O: Caesarian section, fibroid uterus, 10 Hrs

relevant Cancer uterus, prolapse uterus, PID.
surgical &
medical 15. Basics about fracture & management. 15 Hrs
conditions
(relevant to 16. PIVD,Potts spine. 05 Hrs
Lab
technician). 17. Oral cavity tumors. 05 Hrs

18. Eye d’s : Cataract, Glaucoma. 05 Hrs

19. ENT:. CSOM, ASOM, Laryngeal tumor, Nasal poyp, DNS. 06 Hrs

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 Details of Curriculum for Second Year
Diploma in Lab Technician

PAPER 1st Hours.

Theory Topics
1 Chemistry of Carbohydrate 05 Hrs
2 Chemistry of Protein 05 Hrs
3 Chemistry of Lipid 05 Hrs
4 Radioisotopes & Their Use in Biochemistry 05 Hrs
5 Principles of Electrophoresis 05 Hrs
6 Liver Function Test 05 Hrs
7 Renal Function Test 05 Hrs
8 Thyroid Function Test 05 Hrs
9 Body Fluid 10 Hrs
10 Quality Control 05 Hrs
2.Clinical 11 Standardization 05 Hrs
Microbiology- 12 Ultraviolet and Visible Light Spectroscopy 03 Hrs
II & 13 Elisa 10 Hrs
Biochemistry- 14 Radioimmunoassay 10 Hrs
II. 15 Polymerase Chain Reaction (PCR) 10 Hrs
16 Chromatography 10 Hrs
17 Spectrometry 03 Hrs
18 Point of Care Testing 03 Hrs
19 Introduction of Electrolyte & Water Balance 03 Hrs
20 Clinical Approach of Electrolyte & Water Balance 03 Hrs
21 Immunochemistry 05 Hrs
22 Automation in Clinical Biochemistry 10 Hrs
23 Collection of specimens 03 Hrs
24 Identification methods for various bacterias 03 Hrs
25 Methods to prepare Identification medias 03 Hrs
26 Lab diagnosis of diarrhoea 03 Hrs
27 Lab diagnosis of UTI 03 Hrs
28 Lab diagnosis of respiratory tract infection 03 Hrs
29 Lab diagnosis of meningitis 03 Hrs
30 Lab Diagnosis of Tuberculosis 05 Hrs
31 Lab diagnosis of wound infection 03 Hrs
32 Bacteriological examination of water & air 03 Hrs
33 Care and handling of lab animals 03 Hrs
34 Preservation of bacteria 03 Hrs

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 Details of Curriculum for Second Year
Diploma in Lab Technician

PAPER 1st Hours.

Theory Topics
35 Antigens and Antibodies 05 Hrs
36 Antigen-Antibody reaction 05 Hrs
37 Introduction and classification of viruses 05 Hrs
2.Clinical 38 Lab diagnosis of virus including cultivation of viruses 10 Hrs
Microbiology- 39 Medically important DNA viruses including HBV 05 Hrs
40 Medically important RNA viruses including HIV 05 Hrs
II &
41 Introduction & classification of fungi 05 Hrs
Biochemistry-
42 Lab diagnosis of fungi 03 Hrs
II.
43 Medically important fungi-I 03 Hrs
44 Medically important fungi-II 03 Hrs
45 Preparation of smears for fungus examination 03 Hrs
46 Media for fungal culture of Fungi 03 Hrs

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 Details of Curriculum for Second Year
Diploma in Lab Technician

PAPER 2nd Hours.

Theory Topics
Instruments in Histopathology lab – 1. For grossing & for 15 Hrs
1 procesing.
Instruments in Histopathology lab – 2. For section cutting 15 Hrs
2 & staining.
3 Receiving of sample in Histopathology 10 Hrs
4 Registration of samples and record keeping 05 Hrs
5 Preservation of samples in Histopathology. 05 Hrs
6 Grossing of general pathology specimens. 10 Hrs
7 Grossing of respiratory system 05 Hrs
1.Histopathology 05 Hrs
8 Grossing of GIT
& 9 Grossing of Hepatobiliary system 05 Hrs
10 Grossing of male gential system 05 Hrs
Cytopathology.
11 Grossing of female genital system 05 Hrs
12 Grossing of breast tissue. 05 Hrs
13 Grossing of Urinary system 05 Hrs
14 Grossing of Bones 05 Hrs
15 Grossing of thyroid and and endocrine glands 05 Hrs
16 Grossing of Brain tissue 05 Hrs
17 Tissue Blocking and section cutting. 10 Hrs
18 Reagents in Histopathology. 05 Hrs
19 Staining of slides in Histopathology I (H & E ). 05 Hrs
Staining of slides in Histopathology II 10 Hrs
20 (Retic/PAS/VG/Amyloid).
21 Paraffin blocks filing. 05 Hrs
22 Slide filing in Histopathology 05 Hrs
23 Specimen mounting & Labeling. 10 Hrs
24 Cataloguing for museum. 10 Hrs
25 Instruments in Cytopathology laboratory. 20 Hrs
26 Receiving of samples in Cytopathology 10 Hrs
27 Preservatives used in Cytopathology 10 Hrs
28 Staining of slides in cytopathology-1: H & E. 20 Hrs
29 Staining of slides in cytopathology -2:Pap / gimsa 20 Hrs
30 Slide Filing of slides in Cytopathology. 10 Hrs

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 Details of Curriculum for Second Year
Diploma in Lab Technician

PAPER 2nd Hours.

Theory Topics
1 Blood Banking - an introduction. 05 Hrs
2 Blood Bank setup and Functioning, sterlization & sancity. 20 Hrs
3 Common Blood groups. 10 Hrs
2. Blood 4 Rare blood groups. 05 Hrs
banking & 5 Genetics & Blood grouping methods. 05 Hrs
6 Cross matching. 10 Hrs
Biomedical
7 Preparation of grouping sera. 05 Hrs
waste
8 Storage of Blood. 10 Hrs
management.
9 Labeling & Maintenance of blood bags. 05 Hrs
10 Transportation of Blood bags. 05 Hrs
11 Preparation of different components of Blood-I 05 Hrs
12 Preparation of different components of Blood-II 05 Hrs
13 Immune sera – Types , production & uses . 05 Hrs
Screening tests done in blood bank – Diseases & 05 Hrs
14 methods- I
Screening tests done in blood bank – Diseases & 05 Hrs
15 methods- II
16 Rh antibody titre. 05 Hrs
17 Coombs test- Direct & Indirect. 05 Hrs
18 Blood transfusion reactions. 05 Hrs
19 Issuing the blood, madico-legal implications. 05 Hrs
20 Disposal of expired blood. 05 Hrs
21 Basics of Biomedical waste managment 05 Hrs

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 Curriculum
for
Practical :- Second Year
Diploma in Lab Technician

Topics
1 Grossing in General pathology
2 Grossing of GIT
3 Grossing of Hepatobiliary system
4 Grossing of Female genital system
5 Grossing of Breast tissue.
6 Grossing of Urinary system
7 Grossing of Bones
8 Grossing of Thyroid and endocrine glands
9 Staining of slides in Histopathology - H & E
10 Staining of slides in Histopathology - PAS
11 Staining of slides in Histopathology - AFB
12 Staining of slides in Histopathology - GIEMSA
13 Processing in Histopathology I
14 Processing in Histopathology II
Practical 15 Processing in Histopathology III
16 Processing in Histopathology IV
17 Blocking in Histopathology I
18 Blocking in Histopathology II
19 Section Cutting in Histopathology I
20 Section Cutting in Histopathology II
21 Section Cutting in Histopathology III
22 Section Cutting in Histopathology IV
23 Making Stain in Cytopathology I
24 Making Stain in Cytopathology II
25 Making Stain in Cytopathology III
26 Making Stain in Cytopathology IV
27 Making Stain in Cytopathology V
28 Staining of slides in Cytopathology- H& E
29 Staining of slides in Cytopathology - PAP
30 Staining of slides in Cytopathology - AFB
31 Staining of slides in Cytopathology - GIEMSA
32 Blood Grouping And Cross Matching I
33 Blood Grouping And Cross Matching II
34 Blood Grouping And Cross Matching III

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 Curriculum
for
Practical :- Second Year
Diploma in Lab Technician

Topics
35 Rh Antibody I
36 Rh Antibody II
37 Coomb’s Test I
38 Coomb’s Test II
39 Component Preparation I
40 Component Preparation II
41 Normal Value
42 Hyper Value & Hypo Value
43 Normal Value
44 Hyper Value & Hypo Value
45 Normal Value
Practical
46 Hyper Value & Hypo Value
47 Normal Value
48 Hyper Value & Hypo Value
49 Normal Value
50 Hyper Value & Hypo Value
51 Normal Value
52 Hyper Value & Hypo Value
53 T3 & T4
54 TSH
55 PRL
56 Centrifuge
57 PH Meter
58 Electrophoresis
59 PCR
60 Thin Layer Chromatography (TLC)
61 Urine Sample
62 Sputum
63 Wourd swab
64 CSF

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 Curriculum
for
Practical :- Second Year
Diploma in Lab Technician

Topics
65 Stool
66 Animal inoculation
67 Bleeding of mice & rabbit
68 Collection of sheep blood aseptically
69 Care and handling of lab animals
70 Introduction and classification of parasites
71 Medically important parasites -I
72 Medically important parasites -II
73 Procedure/Method of stool examination
74 Preparation & staining of blood films for haemoparasite
75 Preparation of blood film for Parasites
76 Staining (Leishman, Geimsa) & Blood smear examination
Practical
77 Demonstration of P.vivax, P. falciparum & filarial worms

78 Preparation of stool smears

(i) Saline
(ii) Concentrated

79 VDRL test
80 WIDAL test
81 Latex agglutination
82 ELISA Test
83 Staining methods for fungus
84 Preparation of smears for fungus examination-I
85 Preparation of smears for fungus examination-II
86 Preparation of media for culture of fungi

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