Final Project Herbal Mask PDF

Download as pdf or txt
Download as pdf or txt
You are on page 1of 31

A Review on:

"TO FORMULATE AND EVALUATE ANTI DANDRUFF HERBAL HAIR MASK"

A Project Report submitted

For the Partial Fulfillment of Degree of

BACHELOR OF PHARMACY
To the Faculty of Engineering and Technology (Pharmacy),

Dr. BABASAHEB AMBEDKAR TECHNOLOGICAL UNIVERSITY, Chh. Sambhaji Nagar

By

Miss. Suchitra Balasaheb Gaikwad


(Final Yr. B. Pharm)

GUIDED BY

MS. KOMAL BALASKAR.

(ASST. PROFESSOR)

_____________________________________________________________________________________

OYSTER INSTITUTE OF PHARMACY.


Nagonachi Wadi, Golatgaon, Jalna road, CHH. Sambhaji nagar.

2023-2024
_____________________________________________________________________________________

Student's Academic Education Society's

OYSTER INSTITUTE OF PHARMACY

NagonachiWadi, Golatgaon, Jalna road, Chh Sambhaji nagar (M.S.) INDIA

CERTIFICATE

This is to certify that project entitled "A Review on: To formulate and evaluate anti
dandruff herbal hair mask" represents the bonafide work of Miss. RUTUJA ARUN MIND
submitted in partial fulfillment of the requirement for the degree of Bachelor of Pharmacy. The work
carried out in Oyster Institute of pharmacy, Chh. Sambhaji nagar, affiliated to Dr. Babasaheb Ambedkar
Technological University, Lonere

Date:

Place: Chh. Sambhaji nagar.

Mr. Suchitra balasaheb gaikwad

Final Year B. Pharm Seventh

semester
___________________________________________________________________________________

Student's Academic Education Society's

OYSTER INSTITUTE OF PHARMACY


NagonachiWadi, Golatgaon, Jalna road, Chh. Sambhaji nagar (M.S.) INDIA

CERTIFICATE

This is to certify that project entitled "A review on : To formulate and evaluate anti
dandruff herbal hair mask " represents the bonafide work of Miss.Rutuja Arun Mind
submitted in partial fulfillment of the requirement for the degree of Bachelor of Pharmacy. The work
carried out in Oyster Institute of Pharmacy, Chh. Sambhaji nagar affiliated to Dr. Babasaheb Ambedkar
Technological University, lonere.

Date :

Place : Chh. Sambhaji nagar

Dr. Jayshri bairagi

I/C PRINCIPAL
_____________________________________________________________________________________

Student's Academic Education Society's

OYSTER INSTITUTE OF PHARMACY


Nagonachi Wadi, Golatgaon, jalna road, Chh. Sambhaji nagar (M.S.) INDIA

_____________________________________________________________________________________

CERTIFICATE

This is to certify that project entitled ""A review on: To formulate and evaluate anti dandruff herbal hair
mask" represents the bonafide work of Miss. Rutuja Arun Mind submitted in partial fulfillment of the
requirement for the degree of Bachelor of Pharmacy. The work carried out in Oyster Institute of
pharmacy, Chh. Sambhaji nagar , affiliated to Dr. Babasaheb Ambedkar Technological University, lonere.

Date:

Place: Chh. Sambhaji nagar

GUIDED BY.

MS. Komal balaskar .


_____________________________________________________________________________________

OYSTER INSTITUTE OF PHARMACY,


NagonachiWadi, Golatgaon, Jalna road, Chh. Sambhaji nagar .

2023-2024
Declaration

I undersigned, Mr. Rutuja Arun Mind B Pharm 7th semester, OYSTER INSTITUTE OF PHARMACY,
Chh.sambhaji nagar entitled Title "A review on:To formulate and evaluate anti dandruff herbal hair
mask " is based on original review work and has not been submitted previously for the award of any
degree or diploma by me to any other university. The project work was carried out and submitted in the
partial fulfillment of requirement of the degree of pharmacy, in the faculty of Engineering and
Technology [pharmacy] of Dr.Babasaheb Ambedkar Technological University Chh. Sambhaji nagar ,
under guidance of Ms.komal balaskar I hereby declare that information given in the project is true as per
my effort to accomplish this review work.

Date:

Place: Chh. Sambhaji nagar

Miss. Rutuja Arun Mind


ACKNOWLEDGEMENT

I would take the opportunity to thank "The Almighty god" And my "Parents" for providing me with such
a wonderful life and making me capable of reaching this position.

I express my sincere thanks to Dr.Manpreetsingh Johar Sir, Honarable chairman, Students academic
education societies.Chh.sambhaji nagar for providing ample facilities and an encouraging working
atmosphere during the course of my study.

I would like to heartly and sincerely acknowledge the efforts of my highly esteemed and respectable
guide MS. Komal balaskar (Oyster Institute Of Pharmacy) for their valuable guidance, constant
appreciate, inspiration regular motivation, encouragement and indeed ample interest in the project
which enabled me to complete my project.

I am also thankful to my friend and colleagues for their charming company, timely help and co-operation.
I am thankful to all juniors and seniors for their direct or indirect help during this project work. I am
especially thankful to Librarian and all non teaching staff member Oyster Institute Of Pharmacy Chh.
Sambhaji nagar for their timely help and caring nature.

DATE:

MS. Komal balaskar


INDEX

Sr.no TOPIC PAGE No.

1 ABSTRACT 3

2 INTRODUCTION 4

3 INGREDIENTS 7

3.1) Amla powder 8

3.2) Reetha powder 9

3.3) Shikshak powder 10

3.4) Rose water 11

3.5) Aloe vera 12

3.6) Coconut oil 13

3.7) Hibiscus powder 14

3.8)Curry leaves 15

4 PROCEDURE 16

5 EVALUATION PARAMETER 16

A) Organoleptic evaluation 16

B)Physicochemical evaluation 16

C)Phytochemical evaluation 17

D)Rheological evaluation 19

E) Stability studies 20

F) Microbial assy 20

6 SUMMARY 23

7 CONCLUSION 24

8 REFERENCE 25
To formulate and evaluate antidandruff herbal hair mask

INDEX OF TABLE

a) Table no. 1 –Ingredient ………………………………………………………..7


b) Table no. 2 -Angle of repose ………………………………………………..20
c) Table no.3 – Microbial assay…………….. …………………………………..20
d) Table no.4 – Organoleptic evaluation ………………………………………21
e) Table no. 5 – Physicochemical evaluation …………………………………21
f) Table no.6 –Rheological evaluation ……........................................22
g) Table no.7 –stability studies …………………………………………………………22

INDEX OF FIGURE

a) Figure no. 1 Stage of hair growth …………………………………………………..4


b) Figure no.2 Causes oh hair …………………………………………………………..5
c) Figure no.3 Amla ………………………………………………………………………..8
d) Figure no.4 Reetha ……………………………………………………………………9
e) Figure no. 5 Shikshak ………………………………………………………………..10
f) Figure no.6 Rose water ……………………………………………………………….11
g) Figure no. 7 Aloe vera ………………………………………………………………..12
h) Figure no.8 Coconut oil ……………………………………………………………..13
i) Figure no.9 Hibiscus …………………………………………………………………….14
j) Figure no.10 Curry leaves ……………………………………………………………….15
k) Figure no.11 Molish test ………………………………………………………………17
l) Figure no.12 Fehlings test ……………………………………………………………17
m) Figure no. 13 Hagers, test ………………………………………………………………18
n) Figure no. 14 Mayer,s test ………………………………………………………………18

Oyster institute of pharmacy Page 2


To formulate and evaluate antidandruff herbal hair mask

ABSTRACT :
Young generation is facing serious hair problems due to many lifestyle changes such as fatigue, stress,
poor diet and different hair coloring techniques. Strengthening hair follicles is important for improving
hair growth and preventing hair loss. Hair is the most fragile part of the body. Healthy hair improves the
appearance of the individual .The most delicate area of the scalp is the hair. A significant and
widespread issue in today's society is dandruff. In that situation, the skin on the scalp produces flakes,
which itches. Chronic scalp condition that is severe because it affects a wide variety of scalp tissue.
Fungal infections are caused by a variety of factors, including an oily scalp and poor hygiene, and they
become more prevalent if hair is not cleaned for a week. Redness, flaking, and itching are signs of this
chronic scalp condition, which can be treated naturally with herbal hair products. The use of natural
herbs like Amla, Neem, and Shikakai is advised in the ancient Indian medical system Ayurveda.Based on
both traditional systems and scientific justification for modern use, Amla, Bhringaraj, Hibiscus, Shikakai,
Ginger, and other herbs were chosen to formulate the anti-dandruff herbal hair mask. [1]

Keyword - Anti-dandruff, herbal, hair mask, promotes hair growth [1]

Oyster institute of pharmacy Page 3


To formulate and evaluate antidandruff herbal hair mask

Introduction :
The definition of a hair, which is an outgrowth from a follicle on the skin, is "improved epithelial
structure formed as a result of keratinization of Germinative cells." The chemical components Carbon,
Nitrogen, Sulphur, and Oxygen are found in keratin, which is the substance that makes up hair. This
cyclical mechanism includes hair shaft synthesis, elongation, and eventual loss. Human hair is made up
of follicles that are in the Anagen, Catagen, Telogen, and Exogen stages.[9][1] If hair is left unwashed for a
week, fungal infections become more prevalent due to an oily scalp and poor hygiene. Redness, flaking,
and itching are signs of this chronic scalp condition, which can be treated naturally with herbal hair
products. If hair is left unwashed for a week, fungal infections become more prevalent due to an oily
scalp and poor hygiene. Redness, flaking, and itching are signs of this chronic scalp condition, which can
be treated naturally with herbal hair products.[9][1]

Fig 1: stage of hair growth

Natural medicines are now used more frequently than ever before because of their safety and
minimized effects as compared to items with a chemical base. Healthy hair can be achieved with the use
of hair care products devoid of dangerous chemicals. For keeping healthy hair, the ancient Indian
medical school of Ayurveda advises using natural herbs like Amla, Neem, and Shikakai.[1] These plants
are proven to boost hair follicles and nourish the scalp, resulting in thicker, more resilient hair. An herbal
hair mask that fights dandruff helps you get rid of itchy, greasy, flaky skin on your scalp and gives you a
healthy, nourished scalp.[1] A hair mask is a type of hair product that deeply hydrates and nourishes hair
from the roots to the tips. By reducing dandruff, herbal antidandruff hair masks help your hair smooth
the itchy, greasy, and flaky scalp, offering you nutritious hair and an aesthetically pleasing, tingle-free
scalp. Also, without endangering or changing hair, it serves as a conditioning agent.[2][3][9]

Oyster institute of pharmacy Page 4


To formulate and evaluate antidandruff herbal hair mask

The main ingredients in the preparation of the herbal mask are Amla, Bhringaraj, Hibiscus, Shikakai,
Ginger, and other herbs have been chosen to create the herbal hair mask based on their traditional uses
and their scientific support for modern applications. The current study report and formulation involves
the creation of a multiherbal hair mask formulation. [2]

Fig no.2: Causes of dandruff

Causes of Dandruff : Dandruff is primarily caused by a dry scalp and other underlying causes. It
affects many people and is a common hair issue. The condition can irritate your scalp and make it itchy
and flaky. Even though it is not a major health risk, it can make you feel awkward or selfconscious. [2]

1.Dry Scalp

2. Lack Of Good Hygiene

3.Skin Condition

Oyster institute of pharmacy Page 5


To formulate and evaluate antidandruff herbal hair mask

Aim : The aim of the current study is to create an anti-dandruff hair mask to reduce dirt and dandruff.
Thus, it is a very good solution for people who seek more natural and gentle hair care product.
Moreover, hair care product that are free from chemical can help to achieve a healthy look and shiny
hair. [5]

Objective :
• Anti-dandruff herbal hair is a hair care product that absorbs deep into hair to provide hydration and
nourishment from the roots to the tips.

• The present study showed that the main ingredients which have been selected for the formulation of
herbal hair mask on the basis of conventional wisdom and scientific evidence is Amla, Neem, Shikakai,
Reetha, Ginger, Bhringraj, Tulsi, and Rose water. Hair masks take care of hair.

• Hair masks take care of hair that provide a holistic hair care that addresses multiple hair concerns.

• Regular use of this herbal hair mask can result in stronger, healthier, and more shining waves.
[5]
• A hair mask is totally free from hazardous substances.

Need of ant dandruff herbal hair mask :


• The Anti-dandruff herbal hair mask in which the ingredients used in the mask have natural anti-fungal
and anti-inflammatory properties that help soothe and heal the scalp. Regular use of this mask can also
improve overall hair health and promote hair growth.

• This makes it a great choice for those who want to address multiple hair concerns at once. Additionally,
the use of natural ingredients ensures that the mask is gentle on the scalp and free from harsh
chemicals.

• The composition of an anti-dandruff herbal hair pack was shown to be risk-free, more effective, and
commercially viable. It also possesses all the positive traits of an ideal herbal hair mask. [5] [6]

Oyster institute of pharmacy Page 6


To formulate and evaluate antidandruff herbal hair mask

Advantage of hair mask : There are many benefits to using a hair mask, and the advantages vary
depending on the ingredients and your hair type. Generally speaking, the advantage of using a hair mask
include:[7]

1) shinier, softer hair

2) added moisture

3) reduced hair breakage and damage

4)less frizz

5)a healthier scalp

6)stronger hair

7)less environmental and product damage

2] Ingredients :
Sr.no. Ingredients Benifits Quantity

1 Amla powder Hair growth and hair loss 1.5 tbsp

2 Reetha powder Remove dandruff 1 tbsp

3 Shikakai powder Help in hair shine softness 2 tbsp

4 Rose water Helping in repairing 1 tbsp


damage hair

5 Aloe vera powder Anti-dandruff antifungal 2 leaves

6 Coconut oil To provide moisture 1tbsp

7 Hibiscus powder Reduce dandruff 2 tbsp

8 Curry leaves powder Prevent thinking of hair 2 tbsp

Table no. 1 : ingredients

Oyster institute of pharmacy Page 7


To formulate and evaluate antidandruff herbal hair mask

3.1 Amla Powder : Amla powder is a natural herbal powder derived from the fruit of the Emblica
Officinalis tree. This fruit has the highest natural vitamin C content, and we use it in several Ayurvedic
medicines. Amla fruits are largely used in Indian medicine. Fruit also used in preparation of inks, hair oils
and hair shampoo it is reported that fixed oil from fruits possesses the property of promoting hair
growth.[10]

Fig 3: amla

 Advantage of amla powder :


1. Amla provides shine and smoothness

2. Work as a natural conditioner

3. Reduce dandruff

4. Treat head lice

5. Hair growth and hair loss

6. Boosting health hair growth

Oyster institute of pharmacy Page 8


To formulate and evaluate antidandruff herbal hair mask

3.2 Reetha :
Reetha provides cooling effects and seems to be particularly beneficial for washing the skin. It preserves
the scalp's hydration and stops the hair from drying out. The advantages of applying Reetha to the skin
are outstanding. It makes the scalp softer and silkier. In addition, it is applied to the head to remove cell
flakes.[9]

Fig 4 reetha

 Advantage of Reetha powder :


1. Prevent dry mess

2. Good for hair growth

3. Reetha increase the silkiness of hair

4. Cleaning properties

5. Strengthening the hair roots

Oyster institute of pharmacy Page 9


To formulate and evaluate antidandruff herbal hair mask

3.3 Shikshak:

Shikakai has a special capacity to wash the scalp without irritating it, making it effective as an anti-
dandruff remedy. It is very beneficial for treating persistent dandruff caused by an excess of scalp oil. It
benefits by taking away excess oil from the scalp. Decrease dandruff when used regularly. One of the
most effective Ayurveda medicines for hair-related issues, including hair fall, is Shikakai. Shikakai aids in
cleaning the scalp of debris and surplus oil and encourages hair development.[11]

Fig 5: shikshak

 Advantage of shikakai Powder


1. Hair growth

2. Cleanser

3. Anti fungal and anti bacterial properties

4. Heals wounds

5. Anti oxidant

6. Control hair fall

Oyster institute of pharmacy Page 10


To formulate and evaluate antidandruff herbal hair mask

3.4 Rose water :


World Journal of Pharmaceutical Research Rose water is the hydrosol portion of the distillate of rose
petals. Rose water, itself a byproduct of the production of rose oil for use in perfume, is used to flavour
food, as a component in some cosmetic and medical preparations,[8] and for religious purposes
throughout Europe and Asia. Rose syrup is made from rose water, with sugar added.[9]

Fig 6 :rose water

 Advantage of rose water:


1. Control oily scalp

2. Reduce dryness

3. Repair hair damage

4. Improve hair growth

5. Reduces dandruff

Oyster institute of pharmacy Page 11


To formulate and evaluate antidandruff herbal hair mask

3.5 Aloe vera :


It is used in many consumer products, including beverages, skin lotion, cosmetics[11] ointments or in the
form of gel for minor burns and sunburns. The name derives from Latin as aloe and vera ("true"). aloe
vera is the most beneficial for our hair.[8]

Fig 7: aloe vera

 Advantage of aloe vera :


1. Stimulate hair growth

2. Add shine and strength

3. Hydrate hall

Oyster institute of pharmacy Page 12


To formulate and evaluate antidandruff herbal hair mask

3.6 Coconut oil :


Coconut oil is edible oil derived from the wick, meat, and milk of the coconut palm fruit. Coconut oil is a
white solid fat melting at warmer room temperature of around 25°C (78 °F), in warmer climates during
the summer months it is[13] clear thin liquid oil. Unrefined varieties have a distinct coconut aroma. It is
used as food oil, hair and beauty treatment and in industrial applications for cosmetics and detergent
production. Due to its high levels of saturated fat numerous health authorities recommend limiting its
consumption as a food.[12]

Fig 8 :coconut oil

 Advantage of coconut :
1. Moisturize

2. Repair damaged hair

3. Prevent dandruff

4. Improves scalp health

Oyster institute of pharmacy Page 13


To formulate and evaluate antidandruff herbal hair mask

3.7 Hibiscus :
Hibiscus is an excellent herb for hair care. Hibiscus is rich in Calcium, Phosphorus, Iron, Vitamin B1,
Vitamin C, Riboflavin and Niacin, which promotes hair growth and decrease premature graying of hair.
This reduces dandruff. Hibiscus exhibits antioxidant properties by producing flavonoids such as
anthocyanins and other phenolic compounds. It can be used to rejuvenate the hair by conditioning it.[3,4]

Fig 9 : hibiscus

 Advantage of Hibiscus:
1. Proper hair care
2. Reduce dandruff
3. Stimulate hair growth
4. Prevent premature greying
5. Conditions hair

Oyster institute of pharmacy Page 14


To formulate and evaluate antidandruff herbal hair mask

3.8 Curry leaves:


Curry leaves contain proteins and beta carotene which help to prevent thinning of hair and hair fall and
helps to restore natural color of hair. Curry leaves are also rich in antioxidants, these anti oxidants
neutralize free radicals and keeps the hair health and strong.[6,7]

Fig 10: curry leaves

 Advantage of curry leaves


1. Promotes speedy hair growth
2. Control hair loss
3. Repair damage hair
4. Reduce dandruff

Oyster institute of pharmacy Page 15


To formulate and evaluate antidandruff herbal hair mask

3 ] Procedure :

 Took the amla powder, 1.5 tablespoon in a bowl.[13]


 Than add Reetha powder, 1 tablespoon.
 Add Shikakai powder, 2 tablespoon.
 Add Hibiscus and curry leaves powder 2 tablespoon
 Add Rose water, 1 tablespoon.
 Add fresh Aloe Vera 2 leaves.
 Add coconut 1 tablespoon.
 Mix it well to form a paste. Apply it on the hair and scalp.[15]
 let the mask sit for 30 mins and rinse it with water.

4 ]Evaluation Parameters of Hair Mask: The following criteria were used to evaluate the
herbal anti-dandruff hair mask that had been prepared.

(A) Organoleptic Evaluation:


Under this evaluation, the formulation is examined using sensory organs like the eyes or nose, and it
covers macroscopic features of the drug or product, such as colour, smell, texture, and appearance. [13,14]

(B) Physicochemical Evaluation: [ 15]


(i) pH: A calibrated digital pH metre was used to measure the pH of the formulation's 1%
aqueous solution.
(ii) Loss on Drying: Place 1.5 g of the drug's powdered form in a porcelain dish that has been
scaled and is flat and thin. Dry in the oven at 100°C or 105°C, checking that the difference in
weight between any two measurements is no more than 0.5 mg. In desiccators, cool, then weigh.
The weight loss is often noted as moisture.
(iii) Ash Content: In a previously lit and tared crucible (often made of platinum or silica), add
2-4 grammes of the pulverised, air-dried material. Spread the substance out evenly, then
gradually raise the heat to 500–6000°C until it ignites and becomes white, showing a lack of
carbon. In a desiccator, cool, then weigh. If carbon-free ash cannot be produced in this way, cool
the crucible and wet the leftover material with approximately 2 ml of water or a nitrate-
saturated solution. Dry in a water bath, then put on a hot plate and light an even flame. Afte 30
minutes of cooling in a suitable desiccator, immediately weigh the residue. Determine the total
ash content in mg per gramme of air-dried material.

Oyster institute of pharmacy Page 16


To formulate and evaluate antidandruff herbal hair mask

(C) Phytochemical Evaluation: [12]


Several experiments were carried out to determine the phytoconstituents present in the product and
how they affect the body. Every plant demonstrates specific phytochemical traits, which have a variety
of advantageous impacts.

a) Carbohydrate detection tests:


(i) Molisch’s Test: A violet ring forms at the intersection of two liquids when a few drops of
alpha naphthol solution in alcohol are added to 2-3 ml of aqueous extract, which is then shaken.
[15]

Fig 11:molish test

(ii) Fehling’s Test: Boil the mixture of the fehling A and fehling B solutions for one minute. Add
the test solution in an equal amount. Heat in a pot of boiling water for 5–10 minutes. Brick red
and then first yellow streaks are seen.

Fig 12:fehlings test

Oyster institute of pharmacy Page 17


To formulate and evaluate antidandruff herbal hair mask

(b) Detection of Alkaloids:

(i) Hager’s test: When using Hagers reagent, 2-3 ml of filtrate produces yellow ppt.

Fig 13: hagers test

(ii) Mayer’s Test: A creamy ppt is produced using 2-3 ml filtrate and a few drops of Mayers
reagent.

Fig 14 : mayers test

Oyster institute of pharmacy Page 18


To formulate and evaluate antidandruff herbal hair mask

(c) Detection of Volatile oils: [13]


When treated with an alcoholic sudan III solution, 2 to 4 gm of hair mask becomes crimson due to the
presence of volatile oils.

(D) Rheological Evaluation: [14]


(i) Tapped Density:

When a container containing a powder sample is mechanically tapped, a higher bulk density known as
"tapped density" results. Following the initial measurement of the powder volume or mass, the
measuring cylinder or vessel is mechanically tapped for one minute while readings are taken on the
volume or mass until little more volume or mass change is seen. The measurement was given in
grammes per millilitre.

Tapped Density = Mass / Tapped Volumes

(ii) Bulk Density: [15]

The ratio of a powder's mass to its bulk volume is known as bulk density. The necessary amount of
powder is dried and poured into a 50 ml measuring cylinder until it reaches the 50 ml mark. The cylinder
is then dropped from a height of 1 inch at intervals of 2 seconds onto a hard wood surface. The
powder's volume is calculated. The powder is then weighed. Repeating this will get average results.

Bulk Density = Mass / Bulk Volume

(iii) Angle of Repose: [15]

It is described as the highest angle that may be formed between the powder pile's surface and the
horizontal flow. A cylindrical tube with both ends open that contains the necessary amount of dry
powder is put on a flat surface. The funnel should then be lifted in order to create a heap. It is noticed
and documented what the heap's height and radius are. The formula may be used to get the angle of
repose () for the approach described above.

θ = tan-1 (h/r)

Where θ – Angle of repose,

h – Height of the heap,

r– Radius of the base

Oyster institute of pharmacy Page 19


To formulate and evaluate antidandruff herbal hair mask

Sr.N Method Height of Radius of Tan θ Average θ =tan- Flow


o. heap (h in heap(r in =H/R 1(h/r) properties
cm) cm ) Tan θ =

1. Funnel 3.5 cm 5.2 cm 0.6730 0.6821 33'5° Good flow


property
Method 3.3cm 4.9cm 0.6734

3.5cm 5.0cm 0.7

Table no.2

(iv) Hausner’s Ratio: [15]

Hausner’s Ratio = Tapped Density / Bulk Density

(E) Stability Studies: [15]


The physical characteristics of the powdered formulation changed after being kept for a while at two
different temperatures (35 0C and 40 0C) and humidity levels. [29]
[15]
(F) Microbial Assay:
Candida albicans was utilised via the cup-and-plate technique to combat fungus. The agar slants were
used to sustain the culture. After the medium had a chance to solidify, 0.25 ml of the test stain was
added. Two 6 mm wells were then formed in the plates. A sample was placed in one well, while Candida
albicans was placed in the other. [30] The plates were incubated for 24 hours at 37 0C. The zone of
inhibition was measured in order to assess the antifungal activity.

Sr.No Test Purpose for detection Result

1. Molish Presence of carbohydrates Positive

2. Fehlings Presence of carbohydrates Positive

3. Mayer's Presence of alkaloids Positive

4. Hager's Presence of alkaloids Positive

Table no.3

Oyster institute of pharmacy Page 20


To formulate and evaluate antidandruff herbal hair mask

 Results and Discussion [17] The following assessment criteria were used to confirm
that the developed hair mask was excellent.

a) Organoleptic evaluation [17 Organoleptic properties like colour,odour,texture


and appearance are evaluated and the results are listed below

Sr. No. Parameter Result

1 Colour Greenish brown

2 Odour Characteristics

3 Texture Fine

4 Appearance Powder

Table no. 4

b) Physicochemical Evaluation [17]


Physicochemical properties like pH, Loss on drying and Ash content are determined and the results are
listed below.

Sr.No. Parameters Result

1 PH 6.7

2 Loss on Drying 1.28%w/w

3 Ash content 28%w/w

Table no.5

Oyster institute of pharmacy Page 21


To formulate and evaluate antidandruff herbal hair mask

c) Rheological Evaluation [17]


Rheological properties like Tapped density,Bulk density and Angle of repose are determined and the
results are mentioned below.

Sr.No. Parameters Observation

1 Tapped Density 0.4

2 Bulk Density 0.39

3 Angle of repose 38.21

4 Hausner’s Ratio 1.32

Table no.6

[17]
d) Stability Studies: The stability result was displayed in table : a change in look, fragrance,
colour, and texture.

Sr.No Parameter Result

1 Alteration in colour Nil

2 Alterations in Nil
odour

3 Alteration in taste Nil

Table no .7

Oyster institute of pharmacy Page 22


To formulate and evaluate antidandruff herbal hair mask

5 ] summary
Over the past few years, there has been a noticeable growth in the use of herbal products by
consumers. according to a study of global hair care market trends. The hair is affected both directly and
indirectly by factors like UV radiation and the use of harsh chemicals. The current study was conducted
to develop an herbal anti-dandruff hair mask that will shine and encourage hair growth in order to solve
this issue. We come to the conclusion that the formulation of the herbal hair mask lessens dandruff
without irritating the scalp or having any other negative effects.

Oyster institute of pharmacy Page 23


To formulate and evaluate antidandruff herbal hair mask

6 ] Conclusion:
This research lists some herbal medicines that have been successfully used to make hair care products.
In a very simple way, a herbal hair mask removes dandruff from the hair. Herbal-based cosmetics are
well-liked for being non-toxic. This hair mask aids in nourishing the scalp's skin. Through the removal of
extra oil from the scalp, it relieves dandruff. Poor hygiene and an oily scalp cause dandruff in the hair.
Regular usage of this mask results in healthy, dandruff-free hair. Natural medicines are now utilised
more frequently than chemical-based ones all around the world since they are safe and have fewer
adverse effects. The use of readily available substances allows for the preparation of herbal
formulations at home. An effort is being made to create a natural anti-dandruff hair mask using the
beneficial properties of plants that are widely accessible and used in hair care products. The herbal anti-
dandruff hair mask's shelf life is confirmed by a stability study. Different assessment criteria, including
organoleptic evaluation, Ph, loss on drying, ash content, colour, odour, texture, phytochemical
evaluation, stability studies, and microbial test, are used, and the results are important. More significant
findings than in the previous batches are seen in batch C. Against Candida albicans, the developed
formulation exhibits antifungal efficacy. This study demonstrates the product's safety. The assessment
research on amla and Shikakai reveals their anti-dandruff properties. This study demonstrates the safety
and efficacy of a herbal anti-dandruff hair mask formulation for cosmeceutical usage. [16][15]

Oyster institute of pharmacy Page 24


To formulate and evaluate antidandruff herbal hair mask

7 ] Reference :

1) https://ijcrt.org/papers/IJCRT22A6058.pdf

2)Seborrheic Dermatitis and Dandruff: A Comprehensive Review


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4852869/

3)https://www.researchgate.net/publication/361809533 Formulation and Evaluation of Herbal Anti


Dandruff Hair Mask

4)C. K. Kokate, A. P. Purohit, S. B. Gokhale "the text book of pharmacognosy" Edition40Nirali Prakashan,
2007 page no. 10.4-10.5

5) www.irjmets.com

6)international Research journal of modernization in engineering technology and science

7)Dandruff as a potential source of DNA in forensic casework


https://pubmed.ncbi.nlm.nih.gov/9670516/

8)Aloe vera producer signs $3m China deal". ABC News. Australian Broadcasting Corporation. 6
December 2005.

9)Suhagia BN, Rathod IS, Sindhu S. Sapindus mukorossi (areetha): An overview. int j pharm sci res., 2011;
2(8): 1905-1913.

10)C. K. Kokate, A. P. Purohit, S. B. Gokhale "the text book of pharmacognosy" Edition40Nirali Prakashan,
2007 page no. 10.4-10.5.

11)Haircare: Include amla, reetha and Shikakai for healthy and happy hair. NDTV FOOD. Anusha Singh
updated: May 10, 2018 Available :https://food.ndtv.com/beauty/haircare-include-amla-reetha-and-
shikakai-for-healthy-and happy-hair-1848507

12) Safflower oil, salad or cooking, high oleic, primary commerce, fat composition, 100 g". US National
Nutrient Database, Release 28, United States Department of Agriculture. May 2016. Retrieved 6
September 2017.

13)Kokate C.K., Purohit A.P. and Gokhale S.B. “Pharmacognosy”, Nirali Prakashan, Pune, Sixteenth
edition, 2001; 242-253.

14)Chandrani D, Lubaina SZ, Soosamma M. A review of the antifungal effect of plant extract vs. Chemical
substances against Malassezia spp. Int J Pharma Bio Sci 2012; 3(3): 773-80.

15) world journal of Pharmaceutical Research

16)Vimaladevi T. Textbook of Herbal cosmetics. 2015; pp. 94-102.


Oyster institute of pharmacy Page 25

You might also like