MP5332

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Sabouraud Dextrose Agar Plate w/Chloramphenicol & Gentamicin MP5332

Intended Use
Recommended for selective cultivation of yeasts and moulds.
Composition**
Ingredients Gms / Litre
Dextrose (Glucose) 40.000
Mycological, peptone 10.000
Agar 15.000
Gentamicin 0.040
Chloramphenicol 0.400
Final pH ( at 25°C) 5.6±0.2
**Formula adjusted, standardized to suit performance parameters

Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Sabouraud Dextrose Agar is is described by Sabouraud (7) for the cultivation of fungi (yeasts, moulds), particularly
useful for the fungi associated with skin infections. Sabouraud Dextrose Agar is Carlier's modification (3). This
medium is also employed to determine microbial contamination in food, cosmetics, and clinical specimens (2).
Mycological peptone provides nitrogenous compounds. Dextrose provides an energy source. High dextrose concentration
and low pH favors fungal growth and inhibits contaminating bacteria from test samples (6). Chloramphenicol and
Gentamicin inhibits most of the bacterial contaminants.
Type of specimen
Clinical samples: skin scrapings, Food samples, Cosmetics samples.

Specimen Collection and Handling


For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). For food
samples, follow appropriate techniques for sample collection and processing as per guidelines (1,8,9). After use,
contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. Read the label before opening the container. Wear protective gloves/protective clothing/eye
protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard
precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be
referred in individual safety data sheets.

Limitations
1. Individual strain of a microorganism may have unique growth requirements with respect to nutrients and physical
conditions. Based on which the growth pattern of each varies on a medium and some even may display significant delay.
2. Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be
carried out in safety cabinet.
3. Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate
the medium for any specific microorganism other than mentioned in the COA based on the user’s unique requirement.
4. Further biochemical tests should be carried out for complete identification.

Performance and Evaluation


Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at
recommended temperature.

Quality Control
Appearance
Sterile Sabouraud Dextrose Agar w/Chloramphenicol & Gentamicin in 90mm disposable plate with smooth surface and
absence of black particles/cracks/bubbles.
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data

Colour of medium
Light amber coloured, clear to slightly opalescent medium.
Quantity of Medium
25ml of medium in 90mm disposable plates
pH
5.40-5.80
Sterility Test
Passes release criteria
Cultural Response
Cultural characteristics observed after incubation at 22-28°C for 48-72 hours.
Organism Inoculum Growth Recovery
(CFU)
Candida albicans ATCC 50 -100 good to luxuriant >=50 %
10231 (00054*) (white
colonies)
# Aspergillus brasiliensis 50 -100 good to luxuriant >=50 %
ATCC 16404 (00053*)
Trichophyton 50 -100 good to luxuriant >=50 %
mentagrophytes ATCC 9533
Escherichia coli ATCC 50 -100 none-poor 0 -10 %
25922 (00013*)
Pseudomonas 50 -100 none-poor 0 -10 %
aeruginosa ATCC 27853
(00034*)

Key : (*) - Corresponding WDCM numbers. (#) - Formerly known as Aspergillus niger
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated
expiry period.

Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical
sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).

Reference
1. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed.,
Washington D.C.
2. Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. AOAC, Washington D.C.
3. Carlier G. I. M., 1948, Brit. J. Derm. Syph., 60:61.
4. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
5. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual
of Clinical Microbiology, 11th Edition. Vol. 1.
6. Murray PR, Baren EJ, Jorgensen JH, Pfaller MA, Yolken RH (editors) 2003, Manual of clinical Microbiology, 8th
ed.,ASM, Washington, D.C.
7. Sabouraud K., 1892, Ann. Dermatol. Syphilol, 3:1061.
8. Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th
Ed., American Public Health Association, Washington, D.C.
9. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,
APHA Inc., Washington, D.C.

Revision : 00/2021

Please refer disclaimer Overleaf.


HiMedia Laboratories Technical Data

In vitro diagnostic medical


IVD device

CE Marking

8°C Storage temperature

2°C

Do not use if package is


damaged

HiMedia Laboratories Pvt. Limited,


23 Vadhani Industrial Estate,
LBS Marg,Mumbai-86,MS,India

EC REP CE Partner 4U ,Esdoornlaan 13, 3951


DB Maarn The Netherlands,
www.cepartner 4u.eu

Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate
office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
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