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SOP For Purity Test

SOP

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Patrick Maulidi
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275 views6 pages

SOP For Purity Test

SOP

Uploaded by

Patrick Maulidi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 6

Seed Services Unit Revision No.

Original
Seed purity analysis procedure PROC
Approved by Date of Approval Page 1 of 6

1.0 Purpose
1.1 To ensure that seed doesn’t devalue due to the presence of mixtures and impurities by determining
the proportion of pure seed, inert matter and other seeds (weed seed).

2.0 Scope
2.1 This standard operating procedure will run from mixing and dividing of submitted sample to the
reporting of results.

3.0 Reference
3.1 Current version of ISTA Hand book
3.2 Seed identification hand book.
3.3 Seed testing procedure
3.4 Seed sampling handbook.

4.0 Definitions
4.1 QAM: Quality Assurance Manager
4.2 HOST: Head of seed testing
4.3 DHOST: Deputy Head of seed testing
4.3 SSU: Seed Services Unit

5.0 Responsibilities and Authority


5.1 QAM
 Supervising and monitoring seed purity testing procedure and staff
 Ensuring that all seed purity testing processes, procedure and reporting are done
according to ISTA standards in order to meet clients needs.
 Ensuring that all equipments required for seed purity test are available and in good
condition.
 Approving seed purity test results.

5.2 HOST
 Supervising seed analysts as described in seed quality manual
 Ensuring that seed analysts are using all relevant documents when conducting seed purity
test.
 Providing leadership guidance to the staff working in the seed testing laboratory.
 Ensuring a safe work environment in the laboratory.
 Production of certificates of analysis to provide timely and accurate results to the clients.

5.3 DHOST
Works hand in hand with the Host by ;
 Supervising seed analysts as described in seed quality manual
 Ensuring that seed analysts are using all relevant documents when conducting seed tests.
 Providing leadership guidance to the staff working in the seed testing laboratory.
 Ensuring a safe work environment in the laboratory.
 Production of certificates of analysis to provide timely and accurate results to the clients.
Seed Services Unit Revision N0. Original
Seed purity analysis procedure PROC
Approved by Date of Approval Page 2 of 6

5.4 Seed Analysts


 Analyse the purity of all seed species submitted to the labolatory through the guidance of
ISTA standards
 Recording purity analysis findings on pre-printed form.
 Making calculation on seed purity results.
 Checking accuracy of required equipment.

6.0 Activities
6.1 Obtaining a working sample.
6.1.1 Ensure that the submitted sample is of appropriate size.
6.1.2 Ensure that the working area is clean.
6.1.3 Ensure appropriate working documents are at hand.
6.1.4 Ensure the equipment used are in good condition and well calibrated
6.1.5 All the necessary equipment required must be gathered for testing as follows
 Seed divider
 Weighing balance (precision and analytical)
 Scalpels
 Petri dishes
 Calculators
 Pens
 Containers
 Magnifiers and working bench lights.

6.1.6 Have knowledge of the prescribed working sample weight for different species.

Crop Submitted sample Working sample


weight (g) weight (g)
Maize 1000 9000
Beans 1000 700
Ground nuts 1000 1000
Soya bean 1000 500
Rice 700 70
Seed Services Unit Revision Original
Seed purity analysis procedure PROC
Approved by Date of Approval Page 2 of 5

6.2 Separation of impurities


6.2.1 Understand preliminary knowledge of plant species and seed structure.
6.2.2 Must have the current version of ISTA rules.
6.2.3 Be able to understand and apply ISTA rules on seed testing.
6.2.4 Impurities to be separated are pure seed, inert matter, other crop seed, weed seeds.

6.3 Component identification


6.3.1 Be able to apply the principles of pure seed definition.
6.3.2 Be familiar with the botanic structures of the seed for easy identification.
6.3.3 Be able to use seed identification reference materials like;
 Microscope
 Internet
 Reference books
 Seed identification handbook.
6.3.4 Other seeds, weed seeds, inert matter and pure seed are put in separate petri dishes
6.3.5 The kind of inert matter are recorded on the working card in the appropriate spaces provided
6.3.6 Other seeds are examined and each kind found is identified.
6.3.7 The identified species are reported using botanical names and number of seeds provided.
6.3.8 The components are transferred in separate containers and carried on a tray to avoid dropping
the

6.4 Weighing of the components


6.4.1 Seed analyst must be weighed in grams to the specific minimum number of decimal places.
6.4.2 Seed analyst must have knowledge of the type of weighing balance depending on size and
sample weight.
6.4.3 weighing of the components is done separately.
Seed Services Unit Revision Original
Seed purity analysis procedure PROC
Approved by Date of Approval Page 4 of 6

6.4.4 Record the weight on the working card to the required decimal place as follows.

Weight of working sample/sub No.of decimal place


sample
Less than 1.000 4
1.000-9.999 3
10.00-99.99 2
100-999.9 1
More than 1000g 0
“&hn hby7g78
{|=q 1westhe remaining pairs.

Seed services unit Revision Original


Seed purity analysis Procedure PROC
Approved by Date of Approval Page 5 of 6

6.8 Rounding procedure


6.8.1 Check if all fractions are within tolerance.
6.8.2 If the fractions are within tolerance add the weights together, calculate percentage and round the

The figures to 1decimal place. ISTA Rules 2023, 3.6.1.3.

Weight of working sample/subsample No. of decimal places


Less than 1.000 g 4
1.000-9.999 g 3
10.00-99.99 g 2
100.0-999.9 g 1
≥ 1000g 0

6.9 Reporting results


6.9.1 Percentage of all components must add up to 100.
6.9.2 Purity analysis results must be given to one decimal place.
6.9.3 Report all the components that are less than 0.05% as Trace or ‘TR’.
6.9.4 Report the percentage of pure seed, other seed and inert matter in the spaces provided on the
analysis report.
6.9.5 If the results of analysis is nil it must be shown as ‘0.0’
6.9.6 Indicate the scientific name of the specie of pure seed, scientific name of each specie
Of other seed and the kind of inert matter according to the current stabilized plant names.
7.0 Records
7.1 Working cards.
7.2 Copies of certificate.
7.3 Equipment calibration data.

8.0 Appendix
8.1 Appendix 1: Purity working card

Seed services unit Revision No. Original


Seed purity procedure PROC
Approved by Date of Approval Page 6 of 6

1. Seed purity working card

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