Section 1 Pages 1-54
Section 1 Pages 1-54
Section 1 Pages 1-54
- notes are based on Laboratory Safety CSMLS Guidelines 9th Edition (O’Grady)
- references from the CSMLS Guidelines 8th Edition (Shematek, Wood, O’Grady) are included
- I will be highlighting key areas, but YOU ARE RESPONSIBLE FOR THE ENTIRE TEXT
- keep your CSMLS Guidelines close – each lab course refers to it, and it is on your national exam
- the course is designed to cover CSMLS safety competencies (MLA and MLT)
Competency profile general medical laboratory technologist - CSMLS. (n.d.). Retrieved March 22, 2023, from https://go.csmls.org/cert/MLT_CP_2016.pdf
INTRODUCTION
Laboratory Safety CSMLS Guidelines 9th ed pages 1-8; 8th ed pages 7-8
- laboratory safety is crucial to protect yourself and everyone you work with
- there are no happy accidents!
- since 1713, when Bernardo Ramazzini published “Diseases of Workers”, we’ve known safety is an issue
- but knowing about a safety problem is only the first step we need to translate that knowledge into action!
- for years, working in a lab was about getting results, safety wasn’t just low priority – it was no priority
- today, worker safety and patient care are now BOTH top priorities - getting results AND staying safe
- learning about safety, and of course practicing it, never ends
- new hazards emerge with new technologies and diseases
- in the clinical lab, cuts are the number one issue, followed by splashes
- allergies, cancer and lab acquired infections are very real dangers in our environment
LEGISLATION
Laboratory Safety CSMLS Guidelines 9th ed pages 9-10; 49-50 ; 8th ed pages 14-17
- an important part of our profession
CSMLS. (2015, January). CSMLS Competency Profile CSMLS. (2016, January). CSMLS Competency Profile
General Medical Laboratory Technologist. Medical Laboratory Assistant.
https://go.csmls.org/cert/MLA_CP_2016.pdf
https://go.csmls.org/cert/MLTG_CP.pdf
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DUE DILLIGECE
- the concept that everything reasonable should be done to protect workers
- a key employer responsibility is to demonstrate their due diligence by taking all reasonable precautions
- not a simple task for an employer as they need to oversee many things
- policies, training, communication, record keeping, proving rule enforcement are important areas
EMPLOYEE RIGHTS
- about any hazards and their effects
- safety training is a good way to learn about hazards
- that’s why we need proentations and refreshers
- other regulations include specific workplace rules (here, the NBCC safety manual, for example), Routine
Practices and Additional Precautions and Blood and Body Substance Precautions
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WORKPLACE HAZARDOUS MATERIALS INFORMATION SYSTEMS (WHMIS)
- both federal and provincial
- updated in 2015 to become more consistent with the Globally Harmonized System
- consists of three major elements:
- Safety Data Sheets (SDS) that provide detailed hazard information and suggested controls
- Workplace Labels on the container of chemical, used provide immediate hazard information
- Training/Education is the most important and provides the ability to understand the hazards
CANADA CRIMINAL CODE BILL C-45
- federal
- makes it a crime (criminal negligence) to fail to provide safe working conditions
OTHER COMMONLY USED STANDARDS
National Fire Protection Association (NFPA)
- provides codes and standards related to fire and chemical safety
American Society of Heating, Refrigerating and Air Conditioning Engineers (ASHRAE)
- concerned with indoor air quality, and so important for laboratory ventilation systems
American National Standards Institute (ANSI)
- standards for eyewashes and showers; important for laboratory ventilation systems; certifies biosafety cabinets
CSA Group
- respirators; fume hoods; ethylene oxide sterilizers; waste material handling; lasers; hearing protection
American Conference of Governmental Industrial Hygienists (ACGIH)
- setting TLVs (see below)
Public Health Agency of Canada (PHAC)
- handling infectious organisms
OCCUPATIONAL HYGIENISTS
Laboratory Safety CSMLS Guidelines 9th ed pages 20-21; 8th ed pages 10-12
- occupational hygienists anticipate, identify, evaluate and control hazards
- use a variety of measurable techniques such as surveys, inspections and reviews (not hearsay)
- many kinds of HAZARDS in a clinical lab:
- like flammability, toxicity, volatility, corrosiveness
- flammable/inflammable both mean something will burn at RT
- volatile describes a liquid that easily becomes a gas
- AKA dosimetry
- measures a smaller area directly around or on a person
- monitors the same things as area monitoring, but better reflect
the exposure of an individual
- dosimeters can be worn as badges or rings
4
References:
Competency profile general medical laboratory technologist - CSMLS. (n.d.). Retrieved March 22, 2023, from https://go.csmls.org/cert/MLT_CP_2016.pdf
CreativityProject Follow. (n.d.). Lab 0-A lab safety training. Share and Discover Knowledge on SlideShare. Retrieved March 22, 2023, from
https://www.slideshare.net/CreativityProject/lab-0a-lab-safety-training-39685045
CSMLS. (2015, January). CSMLS Competency Profile General Medical Laboratory Technologist. https://go.csmls.org/cert/MLTG_CP.pdf
CSMLS. (2016, January). CSMLS Competency Profile Medical Laboratory Assistant. https://go.csmls.org/cert/MLA_CP_2016.pdf
Government of Canada, C. C. for O. H. and S. (2023, March 22). Canadian Centre for Occupational Health and Safety. Retrieved March 22, 2023, from
https://www.ccohs.ca/
Government of Canada, C. C. for O. H. and S. (2010, July 12). The Young Workers Zone : Teaching Tools : Sociability issues: Canadian Occupational Health
and Safety (OH&S) laws. The Young Workers Zone : Teaching Tools : Sociability Issues: Canadian Occupational Health and Safety (OH&S) Laws. Retrieved
March 22, 2023, from http://www.ccohs.ca/teach_tools/social/laws.html
Karim, N., & Choe, C. K. (2000, December). Laboratory accidents--a matter of attitude. The Malaysian journal of pathology. Retrieved March 22, 2023, from
https://www.ncbi.nlm.nih.gov/pubmed/16329540
O’Grady, E. (2022). Laboratory Safety CSMLS Guidelines (9th ed.). CSMLS Canadian Society for Medical Laboratory Science.
Pityn, P. J. (2008, March). Hygiene at work: An engineering perspective on the development of hygiene science. The Canadian journal of infectious diseases &
medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale. Retrieved April 5, 2023, from
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2605857
Refusing unsafe work: A step by step guide. Canadian Union of Public Employees. (n.d.). Retrieved March 22, 2023, from https://cupe.ca/refusing-unsafe-work-
step-step-guide
Safety+Health. (2017, January 19). Exposure limits: Pels and tlvs. Safety+Health. Retrieved April 5, 2023, from
https://www.safetyandhealthmagazine.com/articles/exposure-limits-pels-and-tlvs-2
Shematek, G., Wood, W., O'Grady, E., & Canadian Society for Medical Laboratory Sciences. (2017). Laboratory safety CSMLS guidelines
Tim Cowher Follow Managing Director. (n.d.). Importance of permissible exposure limits (pels). Share and Discover Knowledge on SlideShare. Retrieved April
5, 2023, from https://www.slideshare.net/TimCowher/importance-of-permissible-exposure-limits-pels
Top 8 emerging diseases likely to cause major epidemics. World Economic Forum. (n.d.). Retrieved March 22, 2023, from
https://www.weforum.org/agenda/2016/04/top-8-emerging-diseases-likely-to-cause-major-epidemics
Yog's Malani Follow. (n.d.). Latex allergy. Share and Discover Knowledge on SlideShare. Retrieved March 22, 2023, from
https://www.slideshare.net/YogeshMalani6539/latex-allergy
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ASSIGNMENT 1
1. Bernardo Ramazzini is considered to be the father of:
a. infections disease medicine
b. pathology
c. occupational medicine
d. a lot of children
2. Until the twentieth century, no one knew that our work affected our health.
3. Because of safety controls, lab professionals do not have to be concerned about developing
laboratory-acquired infections.
4. There continues to be new diseases that could affect the health of a laboratory scientist.
5. Burns are the most common lab accident.
6. Allergies and sensitivities are known laboratory hazards.
7. If you don’t look for ways to improve safety, safety won’t improve.
8. Worker safety and patient care are now both top priorities when working in a lab.
9. Due diligence means everything reasonable should be done to protect workers.
10. Due diligence is an ideal the workplace strives for, but it is not measurable.
14. fix or report hazards to supervisor 15. establish a health & safety committee
16. train employees 17. use PPE
18. ensuring PPE is worn 19. investigate accidents
20. report critical injuries to government agency
21. flow the safety rules for the procedures performed
23. A laboratorian consistently avoided wearing his gloves despite safety training and supervisory
corrections. If the laboratorian got an infection on his hand, would the employer be at fault?
a. yes
b. no
24. Safety issues that cannot be solved by the employee should be reported to:
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The next questions use the following as the answer key:
a. federal government b. provincial government c. municipal government
36. What is the safety legislation and regulatory agency in New Brunswick?
a. NBCC Joint Health and Safety
b. Worksafe NB
c. Canadian Centre for Occupational Health and Safety
d. OSHA
41. Which of the following organizations is NOT responsible for ventilation standards (includes air
quality, fume hoods and biosafety cabinets)
a. ANSI
b. ASHRAE
c. CSA Group
d. PHAC
46. What are the four things an occupational hygienist does involving hazards?
a. tolerate, transfer, treat or eliminate them
b. anticipate, identify, evaluate and control them
c. document, assign blame and prosecute incidents relating to them
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The next questions use the following as the answer key:
a. true b. false
47. An occupational hygienist can always find a way to eliminate workplace risk.
48. Hazards can be identified and evaluated by walk-through surveys, procedure review, safety
data sheets, incident reports, equipment manuals, maintenance records, inspections and
literature reviews.
49. Hazards can be identified and evaluated by Facebook and friend’s opinions
The next questions ask you to classify the hazard by using the following as the answer key:
a. chemical b. biological c. physical d. ergonomic e. psychological
50. the computer is shoved in the corner, and you have to stretch to reach the keyboard
51. electrical cords for some laboratory instruments run across the floor
52. a busy but understaffed laboratory
53. the handwashing sink has low water pressure and no soap
54. the strong smell of formaldehyde is in the hall before you enter the lab.
The next questions ask you define terms and use the following as the answer key:
a. will burn below 100oC b. will not burn c. becomes a gas easily
61. dosimetry
RISK ASSESSMENTS
- risk assessments are legal requirements for the workplace
- the level of risk is calculated by:
- the frequency of hazard exposure (number of workers and how often they are exposed)
- the severity of the consequences (how bad the injury could be)
- the probability of the hazard to cause injury
- can be converted to numerical values to get a more comparable ideas of the risk involved
- charts in the CSMLS 9th Ed Guidelines pages 117-119; 8th Ed pages 32-34 give some actual examples
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LOSS CONTROL
Laboratory Safety CSMLS Guidelines 9th ed page 8; 15; 116; 8th ed pages 8-9
- labs operate on a budget, which we must be respectful of
- safety shouldn’t cost more in the long run, it should actually save money
- deciding what presents a safety risk is the first stage in knowing how to make the lab safer
TOLERATE / ACCEPT THE RISK - the chance of something happening is really low
TREAT / REDUCE THE RISK - change the procedure so it’s less dangerous (like
substituting a less hazardous chemical or using a
safer piece of automation
TRANSFER THE RISK - send test to another facility with better safeguards
- loss control programs are anything done to prevent or lessen the risk
- loss prevention stops the risk from happening
- loss control lessens the risk
- accident cost analysis can help prove good safety practice is economical
Importance of a sound health and Safety System. Intesafety. (2018, September 13). Retrieved April 5, 2023, from https://intesafety.co.nz/importance-of-a-sound-health-and-safety-system/
- OHSMS is a group effort, but the ultimate responsibility falls on the employer
- good leadership is necessary for a good safety culture
- leadership commitment to safety should be genuine and visible, encouraging employees to buy in
- some safety commitments are easy to see as positive
- open communication, provision of resources and recognition of improvement
- some safety commitments can seem less positive, but they are just as important responsibilities
- enforcement of policies, implementing risk assessment and new controls and including safety on
performance appraisals
- part of the OHSMS is hazard assessment
- assessment should be performed by those who do the task, and have been trained in hazard assessment
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- a cornerstone of a good safety culture is that all injuries are preventable
- everyone has the- responsibility of maintaining safety
- employees need to be engaged in safety and participate ]
- employers and employees may need to intervene when they see unsafe acts
- all incidents must be reported to find out their cause, and prevent further accidents
- working unsafely cannot tolerated
HAZARD CONTROL
Laboratory Safety CSMLS Guidelines 9th ed pages 16-19; 8th ed pages 12-13; 21-25
- we identify hazards and risks so we can find ways to control them
- remember, injuries are preventable
- as the hazards and risks are documented, the controls have to be identified, too
- the best way to control hazards is to remove or replace the hazard altogether
- if that isn’t possible, three classes of controls can be used
Controlling risks. WorkSafeBC. (2021, August 10). Retrieved March 22, 2022, from https://www.worksafebc.com/en/health-
safety/create-manage/managing-risk/controlling-risks Work site communication. Grainger KnowHow. (n.d.). Retrieved April 5, 2023, from https://www.grainger.com/content/qt-399-worksite-
com
References:
Adam Killian Follow. (n.d.). Risk assessment. Share and Discover Knowledge on SlideShare. Retrieved April 5, 2023, from
https://www.slideshare.net/AdamKillian/risk-assessment-57468222?next_slideshow=1
Competency profile general medical laboratory technologist - CSMLS. (n.d.). Retrieved March 22, 2023, from https://go.csmls.org/cert/MLT_CP_2016.pdf
Government of Canada, C. C. for O. H. and S. (2023, March 22). Canadian Centre for Occupational Health and Safety. Retrieved March 22, 2023, from
https://www.ccohs.ca/
O’Grady, E. (2022). Laboratory Safety CSMLS Guidelines (9th ed.). CSMLS Canadian Society for Medical Laboratory Science.
Que Tomeyz Follow. (n.d.). Risk management. Share and Discover Knowledge on SlideShare. Retrieved April 5, 2023, from
https://www.slideshare.net/Qurratu92/risk-management-18244112
Shematek, G., Wood, W., O'Grady, E., & Canadian Society for Medical Laboratory Sciences. (2017). Laboratory safety CSMLS guidelines
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vtsiri Follow. (n.d.). Job safety analysis. Share and Discover Knowledge on SlideShare. Retrieved April 5, 2023, from https://www.slideshare.net/vtsiri/job-safety-
analysis-3791277
ASSIGNMENT 2
The next questions use the following as the answer key:
a. hazard b. risk
7. 8. 9. 10.
14. To best meet employee OHS information needs, a supervisor rely on:
a. the induction programme attended when hired
b. a thorough set of policies and procedures
c. regular monthly employee OHS newsletters
d. employee participation in risk assessment and similar exercises
17. How many major steps in a task are recommended for a Job Hazard Analysis?
a. 1-4
b. 5-10
c. 11-15
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18. Organize the following steps of a Task Hazard Analysis (as described in your CSMLS 9th Ed pg 18;
CSMLS 8th Ed Guidelines, chapter 5):
1. verify the effectiveness of controls
2. review available control measures
3. list the major steps of the task
4. identify all equipment used and procedures followed
5. identify potential hazards at each step
a. 2, 5, 1, 4, 3
b. 3, 4, 5, 2, 1
c. 4.1, 2, 3, 5
d. 1, 3, 5, 4, 2
e. 5, 3, 1, 4, 5
19. Which of the following statements is likely to result in an injury to the operator?
a. selecting the right tool for the job
b. wearing safety goggles or glasses
c. using a tool with loose handles
d. keeping cutting tools sharp
For the next questions use the following as the answer key:
a. probability b. severity c. frequency d. risk
26. Replacement of a sick worker is the only cost involved with occupational accidents.
27. Anything that modifies work to reduce or eliminate risk is considered as “loss control”
28. Because safety equipment can be expensive, loss control and reduction studies recommend
fewer safety precautions in the laboratory.
29. do this when the risk from the hazards outweighs the results
30. do this when there are safer options available
31. do this when another testing center has the ability to reduce the risk
32. do this when the risk is so low there will probably never be an accident
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33. What is the very best way to handle hazards?
35. There are many standards available to help guide OHSMS goals.
36. Occupational health and safety should be embedded in all aspects of the workplace.
37. Because OHS is part of everyone’s job, we do not have to define and designate specific roles
and responsibilities.
38. All safety incidents and accidents are the fault of the employer/manager.
39. All injuries are preventable.
40. Incidents are reported to assign blame.
41. An employee cannot be fired for unsafe work practices.
42. A student cannot be removed from a laboratory because of unsafe work practices.
43. A good safety culture begins with good leadership but needs everyone’s participation.
45. Which of the following are ways management can show commitment to OHSMS?
a. include safety in performance reviews
b. regularly communicate to staff about safety and safety policies in the workplace
c. take action if safety policies are not followed
d. all of the above
46. A lab manager encouraged their staff to try several brands of gloves, all with the same level of barrier
protection. A particular brand was unanimously chosen for their fit and comfort. The brand chosen was
double the price of the others. The second choice was cheaper. What should the manager do?
a. try to find a cheaper brand the staff likes just as well
b. tell the staff the price difference and ask if it is worth it
c. buy the first choice gloves if no others can be found that are as good
d. buy the second choice because of the price
e. a, b and c are correct
73. Requiring two staff instead of one to lift a heavy package of coins is an example of:
a. an elimination of risk
b. a substitution control
c. an administrative control
d. a form of PPE control, because it reduces the chance of dropping the object
80. Employee training orientation should include all of the following EXCEPT:
a. hazard reporting
b. OHS policies, rights and responsibilities
c. emergency response procedures
d. operating procedures for all equipment used
88. Who has responsibility for health and safety at your place of work?
a. the client and main contractor only
b. self- employed contractors only and employees
c. employers, employees and sub-contractors
d. everyone at your place of work no matter who employs them
DONNING
GLOVES
- compete hand hygiene, making sure hands are dry
- remove gloves from dispenser and examine for cuts or tears
- hold the glove at the wrist, line up the fingers and thumbs
- insert your hand and pull the cuff over your wrist
- with your gloved hand, place fingers under the wrist edge of the second glove and slip it on
Disposable gloves donning infographic free vector. Nohat. (2020, May 8). Retrieved March 23, 2022, from https://nohat.cc/f/disposable-gloves-donning-infographic-free-vector/4b3087527cab488e9128-202005080100.html
FULL PPE
1. perform hand hygiene
2. put on gown or labcoat
3. put on mask or respirator
4. put on eye protection
5. put on gloves
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DOFFING
CONTAMINATED GLOVES
- pinch and hold the OUTSIDE of the glove near the wrist
- peel downwards, away from the wrist, turning the glove inside out
- pull the glove away until it is removed, holding the inside-out glove with the gloved hand
- with your ungloved hand, slide your fingers under the wrist of the remaining glove
- do NOT touch the outside of the glove
- peel downwards, away from the wrist, turning the glove inside out
- continue to pull the glove down and over the inside-out glove being held in your gloved hand
- dispose of gloves
- perform hand hygiene
How to Don and Doff PPE correctly: (n.d.). Retrieved April 10, 2023, from https://vshopg.tk/ProductDetail.aspx?iid=414332795&pr=26.88
FULL PPE
- be careful of self contamination
1. remove the dirtiest first – your gloves
2. remove lab coat or gown - still pretty dirty
3. hand hygiene
(been touching dirty gloves & lab coat)
4. remove eye protection with your nice clean hands
5. remove mask or respirator
6. hand hygiene
- some protocols call for hand hygiene between each step
YES NO NO YES NO
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- ensure a clear path to emergency equipment and exit routes
- always use mechanical pipette devices (never mouth pippette – eew!)
- use routine practices and additional precautions when using biological material (not just blood)
- don’t touch clean items (like a keyboard or doorknob) with contaminated gloves
- wear gloves when they are needed, not all the time
- assume any unknown substance to be hazardous
- use a chemical fume hood for volatile, toxic or flammable chemicals
- use a biosafety cabinet when there is a danger of biohazardous aerosols
- confirm proper use of fume hoods and biosafety cabinets before use
- keep unauthorized personnel out of the lab
- properly label ALL glassware and other vessels with a legible identification label
- NO FOOD (including cough drops or gum) or drink in the lab
- no CELL PHONES or headphones, as they can spread contamination when taken from the lab
- keep lab clean and ensure only necessary chemical or biologicals samples are in the lab
- do not store samples or reagents on the floor
- walk, don’t run, in the lab
- routinely inspect work areas and equipment for hazards
- act upon safety incidents promptly
- inform your supervisor of hazardous situations you can’t correct yourself
- never work alone, ensure contact with nearby personnel in case of emergency
- maintain visual and audible contact with co-workers, so no loud radios or personal headsets
BEFORE LEAVING THE LABORATORY
- before leaving the lab, return unused samples, reagents and equipment to storage, label and store wastes
- decontaminate work areas
- make necessary repair arrangements and lock out unsafe equipment
- turn off water, gas, compressed air and heating devices
- leave your labcoat and other PPE in the designated area
- wash hands
- close and lock lab
References:
Bernardes, G. C. S., Godoi, A. P. N., de Almeida, N. A., Nogueira, L. S., & Pinheiro, M. B. (2021, April 30). Doffing personal protective equipment in times of
covid-19. Revista brasileira de medicina do trabalho : publicacao oficial da Associacao Nacional de Medicina do Trabalho-ANAMT. Retrieved April 28, 2023,
from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100761/
British Columbia Centre for Disease Control. (n.d.). Retrieved April 28, 2023, from http://www.bccdc.ca/Health-Professionals-
Site/Documents/COVID19_MOH_BCCDC_Doffing.pdf
Department of Emergency Medicine | Saint john. (n.d.). Retrieved April 28, 2023, from https://sjrhem.ca/covid-19-infection-protection-and-control/
Disposable gloves donning infographic free vector. Nohat. (2020, May 8). Retrieved March 23, 2022, from https://nohat.cc/f/disposable-gloves-donning-
infographic-free-vector/4b3087527cab488e9128-202005080100.html
Eastern Health. (n.d.). Retrieved April 28, 2023, from https://www.easternhealth.ca/wp-content/uploads/2021/06/Donning-and-Doffing-PPE-sidexside-poster-
11x17-Provincial.pdf
Frankadmin. (2020, May 13). Donning and doffing PPE. IAM Spectrum. Retrieved April 10, 2023, from http://iamaw1231.ca/2020/05/13/donning-and-doffing-
ppe/
How to Don and Doff PPE correctly: (n.d.). Retrieved April 10, 2023, from https://vshopg.tk/ProductDetail.aspx?iid=414332795&pr=26.88
HR Safety Consulting, LLC. Facebook. (2020, May 19). Retrieved March 23, 2022, from https://www.facebook.com/HR-Safety-Consulting-LLC-
232102020162960/photos/pcb.3204006826305783/3204006486305817/
O’Grady, E. (2022). Laboratory Safety CSMLS Guidelines (9th ed.). CSMLS Canadian Society for Medical Laboratory Science.
PPE Resources. Shared Health. (2023, April 25). Retrieved April 28, 2023, from https://sharedhealthmb.ca/covid19/providers/ppe-resources/
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Procare, O., & Procare, O. (2020, April 15). We are advising all of our carers with the best practices at this time of crisis. ProCare Nursing Ltd - Cardiff - Wales.
Retrieved March 23, 2022, from https://www.procarenursing.co.uk/advising-carers-best-practices-time-crisis
Putting on (donning) Personal Protective Equipment (PPE). (n.d.). Retrieved April 28, 2023, from https://albertahealthservices.ca/assets/Infofor/hp/if-hp-ipc-
donning-ppe-poster.pdf
Putting on Personal Protective Equipment (PPE). (n.d.). Retrieved April 28, 2023, from https://www.publichealthontario.ca/-/media/documents/ncov/ipac/ppe-
recommended-steps
Taking off (doffing) Personal Protective Equipment (PPE). (n.d.). Retrieved April 28, 2023, from https://albertahealthservices.ca/assets/Infofor/hp/if-hp-ipc-
doffing-ppe-poster.pdf
Residents orientation handbook - west park healthcare centre. (n.d.). Retrieved April 11, 2023, from https://www.westpark.org/-
/media/Files/PDFs/ResidentsOrientationHandbook2017.ashx?la=en
Shematek, G., Wood, W., O'Grady, E., & Canadian Society for Medical Laboratory Sciences. (2017). Laboratory safety CSMLS guidelines
University of Calgary Medical Students Year 2 2017 Jun 30
ASSIGNMENT 3
1. Personal Protective Equipment is required when:
a. employers suffer an injury
b. the employees suffer an injury
c. an employee asks for it
d. engineering, work practice, and administrative controls do not provide sufficient
protection against hazard
16. Laboratory coats must be taken home to be bleached and cleaned on a regular basis.
17. Wearing the required PPE is one of the required duties of your job.
18. Lab coats and gowns button, snap or tie in the front.
19. It is standard practice to roll up your lab coat sleeves when it is warm in the lab.
20. If a lab procedure is expected to produce spatter, a disposable apron may be used in lieu of a
labcoat.
21. Disposable sleeves ae appropriate to wear over your labcoat while working in a BSC.
22. If a face shield covers your eyes, you aren’t required to wear eye protection.
23. If you wear prescription lenses, you do not need additional eye protection against
occupational eye hazards.
24. Working with class III B goggles requires specialized safety goggles.
25. There is no single work glove that is effective for all tasks.
26. Hives, itchy eyes and asthma-like breathing issues when in contact with latex can be
signs of a latex allergy.
27. Insulated gloves are recommended for phlebotomy.
28. You should wash your hands after removing gloves.
29. Any closed toes shoes are acceptable in the lab.
30. Surgical masks protect products or specimens from YOU contaminating THEM.
31. Wearing a respirator is a better control than using a fumehood.
32. Respirators are recommended for people with respiratory issues such as asthma.
33. If a respirator does not fit properly, the worker cannot handle hazardous materials that require
a respirator.
23
34. You are about to perform a procedure with vigorous mixing of a hazardous microorganism, and it is
difficult to prevent splashing and aerosols. What should you do?
a. wear a lab coat and gloves
b. wear a lab coat, gloves and safety goggles
c. wear a lab coat, gloves, safety goggles and work in a BSC
d. wear a lab coat, disposable sleeve protectors, gloves, safety goggles and work in a BSC
35. If there is a risk of materials flying into your eyes, you should wear:
a. tinted welding goggles
b. laser safety glasses
c. chemical-resistant goggles
d. impact-resistant goggles
36. Which of the following statements about Personal Protective Equipment are correct?
a. PPE must be worn and stored only inside the laboratory
b. PPE must be chosen based upon the work being completed
c. employees utilizing PPE must be properly trained
d. all of the above
37. It is best to learn about hazards when you are actually working with them, not before.
38. Emergency procedures must be posted in the lab and reviewed, and you must know them.
a. b. c. d.
49. Which of the following statements about Personal Protective Equipment are CORRECT?
a. PPE must be worn and stored only inside the laboratory
b. PPE must be chosen based upon the work being completed
c. employees utilizing PPE must be properly trained
d. all of the above
50. What PPE is mandatory for all the personnel entering / working in the laboratory?
a. dark goggles and dust masks
b. safety glasses, fully enclosed footwear and lab coat
c. dust masks and disposable shoe covers
d. safety glasses and shoe covers
e. lab coat and steel capped shoes
63. If your cell phone rings while you are in a laboratory, you should:
a. answer only if there are no flammable materials stored nearby
b. answer only if it is safe to remove your dusk masks or chemical shield
c. answer only after taking off your gloves
d. terminate the call or answer it outside, after removing gloves
e. you wouldn’t know because cell phones are not permitted in the laboratories
COMPRESSED GASES
Laboratory Safety CSMLS Guidelines 9th ed page 61; 8th ed page 84
Nexreg. (2019, April 11). Health canada- WHMIS
2015 ‣ nexreg. Nexreg. Retrieved April 25, 2023,
from https://www.nexreg.com/health-canada-whmis-
2015
- gas molecules move freely and expand to completely fill their container and exert pressure upon it
- gases are compressed so more can be stored in the same space
- compression increases the pressure the gas exerts, and that pressure itself is a hazard
- compressed gas is stored in specially designed canisters (cylinders) made of metal with safety coatings
- it must be very heavy to resist the pressure inside
- gas release is controlled through valves on the neck, making the neck to most fragile part
HAZARDS
Explosive Potential
- if a gas canister is heated, the gas inside exerts more and more pressure, and may overwhelm the canister
- do not store in hot locations
- a damaged cannister has a weak spot, through which gas can suddenly and violently release
- never use a damaged cylinder!
- breakage of the head valve or rupture of the cylinder can result in violent gas release
Oxygen Deficient Atmosphere
- gas released into an enclosed room can create an oxygen deficient atmosphere (l19.5% or less)
- oxygen monitors may be required
Gas Embolism
- sudden high pressure gas release can puncture the skin and cause a serious, even fatal embolism
Chemial Itself
- the gas itself be a hazard and can result in a toxic, flammable or corrosive atmosphere
PRECAUTIONS
- never accept gas cylinders that are not properly identified
- secure cylinders firmly in the upright position during storage, handling and transport
- remove regulator and attach safety valve cap for storage or transport
- secure cylinder to an architectural support (do not use nylon or other combustible material)
- store in a well-ventilated room with a 1 hour fire restraining wall (check fire codes)
- store in a separate from the rest of the lab (only cylinders in use should be in the lab)
- store oxidizing gas separate from non-oxidizing gas
- store full cylinders separately from empty cylinders
- tag empty cylinders, replace safety valve cap and store waste canisters until disposal
- transport cylinder secured on a 4-wheel dolly (never lift by the neck or drag) with the valve safety cap on
- verify the regulator, pressure rating and connector are correct
- never use adaptors to make a regulator for one gas fit on a different type of gas cylinder
- do not use regulators that don’t’ thread snugly – don’t use lubrication or sealing tape
- tighten regulators with the correct wrench
- check for leaks with a soap solution
- use as directed, being careful to slowly open the diaphragm valve, then the control valve, standing to the side
- close main cylinder valve, bleed out gas until the pressure is “0” when not in use
27
CRYOGENIC HAZARDS
Laboratory Safety CSMLS Guidelines 9th ed page 62; 8th ed pages 84-85
Cryogenic tanks. BCGA. (2022, August 17).
Retrieved April 25, 2023, from
http://www.bcga.co.uk/pages/index.cfm?page_id=8
9&title=cryogenic_tanks
ELECTRICAL HAZARDS
Laboratory Safety CSMLS Guidelines 9th ed page 62-63; 8th ed pages 85-86
Danger electricity sign. Stocksigns. (2022, June 20).
Retrieved April 25, 2023, from
https://www.stocksigns.co.uk/items/hazard-danger-
electricity-sign/
HAZARDS
Fire
- electricity is a potential ignition source and causes 25% of fires
Tissue Damage
- electricity can burn human tissue along path of current flow
- electricity can cause muscle contraction (the “can’t let go” phenomenon) and ventricular fibrillation
- low voltage and low current can be fatal - a current of 25 milliamperes from a 100 volt power source can kill
- the risk and severity of human injury depends on:
- length of exposure
- the voltage and the current
- the pathway through the body (not good to go thru the heart!)
- moisture content of skin (easier to go thru wet skin, because wet skin has less resistance)
- susceptibility of the individual, especially those with heart problems
PRECAUTIONS
- in the event of an accident, turn off the electricity at the circuit breakers and fuse boxes FIRST!!
- never use adaptors that make a three-prong plug a two prong plug, or remove the ground pin!!
- third prong grounds (provides a path back to the main electrical panel in case of a fault)
- avoid the use of extension cords
- unplug by grasping the plug, not the cord
- inspect cords and equipment every time you use them
- electrical equipment needing repair must be taken out of service until repairs are made
- if electrical equipment becomes wet, shut off power at the circuit breaker before clean-up
- use ground fault circuit interrupters (GFCI) should be used, especially if moisture could be present
- smoke or burning smell (or XS heat) from electrical equipment? Cut off power ASAP
28
- do not place cords across (or near to) pedestrian traffic
- use only non-sparking electrical equipment in flammable atmospheres
- use class C fire extinguishers on electrical fires
- use only CSA approved electrical equipment (approved for what you are using it for!)
- use only licensed electricians
TEMPERATURE EXTREMES
Laboratory Safety CSMLS Guidelines 9th ed page 67; 8th ed pages 92-93
- heat sources include autoclaves, drying ovens, hotplates, wax embedding centres, wax trimmers etc
HEAT HAZARDS
- may cause burns from direct contact with hot surfaces, hot steam, hot liquid
- may cause hyperthermia from working too long in warm rooms
HEAT PRECAUTIONS
- wear heat resistant gloves when handling hot items
- allow sufficient time for autoclaves to exhaust steam before opening
- ensure autoclave items are cool before attempting removal
- take frequent breaks and stay hydrated in warm environments
- cold sources include cryogenic fluids, dry ice, freezers, refrigerators, etc.
COLD HAZARDS
- may cause burns from direct contact with cryogenic fluids
- may cause hypothermia from cold rooms and freezers
- may cause increased susceptibility to vibrations
COLD PRECAUTIONS
- wear insulating gloves when moving items into, in or out of the freezer or cryogenics
- wear warm clothing when working in cold rooms for prolonged periods
NOISE
Laboratory Safety CSMLS Guidelines 9th ed page 67; 8th ed page 93
- noise is unwanted sound
- sound is measured in decibels (dB), which is a logarithmic scale
- so a small increase in decibels means a large increase in sound energy
- the A-weighted decibel scale describes sound as it applies to human hearing
- noise is evaluated with a sound meter
- over 90 decibels, hearing protection must be provided, the employee needs an annual physical and
signage for high noise must be in place
HAZARDS
- interference with communication (impacts safety and job performance)
- physiological effects (hearing loss, ear pain, tinnitus)
- physiological effects (disruption of sleep or relaxation)
PRECAUTIONS
- evaluate noise levels of equipment before purchasing, and purchase the quietest equipment possible
- place noise-generating equipment far from work stations
- consider engineering controls (absorptive material of surfaces, noise barriers etc)
- maintain equipment properly
- use additional PPE (earplugs and headphones) as required
PRESSURE
Laboratory Safety CSMLS Guidelines 9th ed page 68; 8th ed page 93
- pressure differences account for many lab accidents, and glass in implicated in injuries
HAZARDS
- unplanned pressure changes can be caused by rapid temperature changes
- like removing samples from cryogenic liquids into room temperature
- unplanned pressure changes can also be caused by chemical reactions inside sealed containers
29
PRECAUTIONS
- use only pressure approved apparatus
- use apparatus with pressure relief mechanisms
- wrap cloth tape around glass vacuum apparatus
- let pressure return to normal after a vacuum desiccator or removal from a cryogenic fluid
- wear eye and face protection near pressure hazards
- open containers slowly
IONIZING RADIATION
Laboratory Safety CSMLS Guidelines 9th ed pages 63-64; 8th ed pages 86-90
File:radiation warning symbol 3.SVG. Wikimedia
Commons. (n.d.). Retrieved April 26, 2023, from
https://commons.wikimedia.org/wiki/File:Radiation_
warning_symbol_3.svg
Gunnells, D. (n.d.). Alara - as low as reasonably achievable. The University of Alabama at Birmingham. Retrieved April 26, 2023, from https://www.uab.edu/ehs/radiation-safety/alara
RADIOACTIVE SPILLS
- most cleanup needs to be performed by qualified staff, from selection of PPE to removing contaminated
materials
MINOR SPILLS
1. inform everyone in the area that a spill has occurred
2. cover spill with appropriate absorbant
3. limit access to the area to those cleaning the spill
4. survey radiation doses in evacuated personnel
5. remove contaminated clothing and footwear, wash and resurvey
6. place clean-up materials in appropriate labeled container
7. monitor area to judge effectiveness of clean-up
8. report spill to nuclear safety officer
MAJOR SPILLS
1. notify and evacuate immediate area and limit movement to contain contamination
2. turn off ignition sources if safe to do so
3. vacate room, close door, post warning, allow aerosols to settle
4. contact radiation safety officer for assistance
References:
Chapter III: University of Florida Radiation Short course Lesley Hines - PPT video online download. SlidePlayer. (n.d.). Retrieved April 26, 2023, from
https://slideplayer.com/slide/6403705/
Decibels explained: Noise Monitoring Services Noise Monitoring Services. Noise Monitoring Services. (2021, February 6). Retrieved April 26, 2023, from
https://www.noisemonitoringservices.com/what-is-a-decibel/
Gas Cylinder/cryogenic liquid training. California State University, Long Beach. (n.d.). Retrieved April 21, 2023, from
https://web.csulb.edu/colleges/cnsm/safety/training/gas.html
Gunnells, D. (n.d.). Alara - as low as reasonably achievable. The University of Alabama at Birmingham. Retrieved April 26, 2023, from
https://www.uab.edu/ehs/radiation-safety/alara
Hearforever best practices in hearing conservation. - ppt download. SlidePlayer. (n.d.). Retrieved April 26, 2023, from https://slideplayer.com/slide/7594086/
How equipment works. How Equipment Works. (n.d.). Retrieved April 26, 2023, from https://www.howequipmentworks.com/electrical_safety
Is the ground wire necessary? Quora. (n.d.). Retrieved April 26, 2023, from https://www.quora.com/What-is-the-purpose-of-earth-wire
Lung cancer causes. Visual.ly. (n.d.). Retrieved May 25, 2022, from http://visual.ly/lung-cancer-causes and Canadian Cancer Statistics Advisory Committee in
collaboration with the Canadian Cancer Society
Mirzaei, M. (2007, December 3). Failure analysis of an exploded gas cylinder. Engineering Failure Analysis. Retrieved April 21, 2023, from
https://www.sciencedirect.com/science/article/abs/pii/S1350630707001744
Nirs.qst.go.jp. (n.d.). Retrieved April 26, 2023, from http://www.nirs.qst.go.jp/db/anzendb/NORMDB/ENG/1_yougosyuu.php
O’Grady, E. (2022). Laboratory Safety CSMLS Guidelines (9th ed.). CSMLS Canadian Society for Medical Laboratory Science.
Pacchioli, D. (2022, November 7). Radiation health risks. Woods Hole Oceanographic Institution. Retrieved April 26, 2023, from
https://www.whoi.edu/oceanus/feature/heath-risks
Shematek, G., Wood, W., O'Grady, E., & Canadian Society for Medical Laboratory Sciences. (2017). Laboratory safety CSMLS guidelines
31
YouTube. (2013, January 2). This is why gas cylinders should be stored in a gas cylinder cage. YouTube. Retrieved April 21, 2023, from
https://www.youtube.com/watch?v=RsNenNTA_Dg
YouTube. (2016, January 24). LEAK test how to use Bubble solution. YouTube. Retrieved April 21, 2023, from https://www.youtube.com/watch?v=h4eoDqqek64
ASSIGNMENT 4
1. Which of the following diagrams represents a gas?
Chapter 8 solids, liquids, and gases. Solids, Liquids, and Gases. (n.d.). Retrieved April 26, 2023, from https://saylordotorg.github.io/text_the-basics-of-general-organic-and-biological-chemistry/s11-solids-liquids-and-gases.html
a. b. c.
29. Before you remove a fitting on a gas cylinder, the most important thing to do FIRST is:
a. check for leaks using a soapy solution
b. lubricate the fitting so it will slip off easily
c. make sure the valve safety cover is close by
d. make sure cylinder valve is off and the exit pressure is zero
32. What is the most commonly used cryogenic fluid used in the clinical laboratory?
a. liquid oxygen
b. liquid helium
c. liquid nitrogen
d. liquid hydrogen
e. liquid carbon dioxide
42. Which of the following affect how severe an electrical risk is?
a. duration of contact
b. voltage
c. current
d. pathway through the body
e. all of them have an effect
43. Why is a person more at risk for an electrical shock if they are wet?
a. the water increases the surface area of the electrical contact
b. the water decreases the resistance of the skin
c. the water increases the resistance of the skin
d. a and c
44. John has an implanted pacemaker to control his heartrate, George has never had a heart issue, except
a broken heart in college. If both are the victims of the same electrical shock, who would be expected
to suffer the most damage?
a. both will have the same damage from the same accident
b. John, who has heart issues
c. George, because he has nothing to live for
47. Why shouldn’t the adaptors shown used in the lab (or really anywhere)?
a. they don’t fit into North American outlets
b. they remove the grounding (n.d.). Retrieved from:
https://i.frg.im/yvK2Tr5/121309623464-0.jpg
c. they can be used!
49. Victims of electric shock are likely to have heart and muscle issues as well as burns.
50. Victims of an electrical shock may not be able to let go of the electrical source.
51. The pathway for an electrical shock is always through the heart.
52. Electrical systems can be an ignition source for flammable or combustible materials.
53. A current of 25 milliamperes from a 100 volt power source can kill.
54. Extension cords and adaptors and adaptors are acceptable to use with lab equipment if they
say “CSA approved”.
34
The next questions use the following as the answer key:
a. true b. false
55. The function of a fuse or circuit breaker is to interrupt the flow of electricity if the circuit
becomes "overloaded"
56. You should know where the circuit breakers are because that is where you stop the source of
electricity in the event of an accident.
57. Outlets in moisture prone areas in the lab should be GFCIs.
58. Since electricity generates heat, it is normal for instruments, power cords and outlets to feel
very warm.
59. You should visually check the equipment you are using every time you use it.
60. Broken or damaged electrical cords and instruments should be labeled “DAMAGED DO NOT
USE” and repaired as appropriate.
61. Avoid damp and wet areas when working with electricity.
62. Use a class A fire extinguisher on an electrical fire.
63. Grasp the plug, not the wire, to unplug an instrument.
100. Which of the following is/are a method to detect radiation exposure or contamination?
a. film badge
b. wipe test
c. TLD dosimeter
d. Geiger counter
e. all of the above
101. If the isotope is placed behind shielding, it can actually create additional
radiation hazards in the form of X-rays/
a. 3H / Plexiglas
b. 14C / Lucite
c. 32P / lead
d. 35S / polystyrene
LASERS
Contraindications and points of attention to consider when using lasers. laser.com. (n.d.). Retrieved April
30, 2023, from https://energy-laser.com/the-history-of-laser-therapy/
HAZARDS 7.top/products.aspx?cname=laser%2Blight%2Bamplification&cid=42
Shematek, G., Wood, W., O'Grady, E., & Canadian Society for Medical Laboratory Sciences. (2017). Laboratory safety CSMLS guidelines pg 91
- avoid direct viewing of the laser and avoid reflections from mirrors, walls etc
- wear the right eye protection for the laser used
- limit access to the laboratory when laser in use
- operate lasers of class III b and above only in posted laser-controlled areas
37
MICROWAVES
- place object to be heated in the centre of the oven
HAZARDS
- heating of tissue (scalds, burns) is the primary hazard
- fires are possible
PRECAUTIONS
- do not use household microwaves in the lab, as they ae not calibrated properly
- provide regular maintenance (clean it, check doors and seals, check oven for leaks)
- do not use metal in the oven as it reflects microwaves ad can cause fires
- do not use tightly covered containers in the microwave and the pressure inside build-up dangerously
- high-powered microwave and radio wave devices (not common in the lab) need extra precautions
- ensure shields and covers are in place
- post warning signs to protect people with pacemakers
ULTRAVIOLET LAMPS
- ultraviolet radiation has a wavelength of between 180-390 nanometres
HAZARDS
- UV is especially damaging to the eye (not great for skin, either)
PRECAUTIONS
- reduce direct and reflected radiation with goggles or face shield with lens filters
- remember UV radiation is reflected by surfaces, like stainless steel
- cover exposed body parts
- turn off UV lights when not in use
EYEWASHES
- the eye wash is the most critical of the wash devices
- a drop of corrosive can cause permanent damage, eyes also a route of entry for biohazards
- see chart in CSMLS 9th Ed Guidelines page 93; 8th Ed page 125
POTABLE EYEWASH
- not connected to running water
- the fluid (usually saline) must be provided
- not usually 15 min worth, so they are only good enough to get to a plumbed eyewash
- must be well maintained to prevent contamination
PLUMBED EYEWASH
- attached to plumbing and so has a permanent water supply
- characterized by 2 spray heads directed at the eyes and a push lever to activate
- flow rate should be 1.5 lpm
- as with all emergency washes, the water should be tepid (16-30 oC) for at least 15 minutes
EYE/FACE WASH
- attached to plumbing and so has a permanent water supply
- eye/face washes have spray heads that are directed at the eye and part of the face
- additional area washed makes eye/face washes preferred
- flow rate should be 11.4 lpm
- as with all emergency washes, the water provide should be tepid (16-30 oC) for at least 15 minutes
DRENCH SHOWER
- used when the hazard is splashed on the body.
- see chart in CSMLS 9th Ed Guidelines page 93; 8th Ed page 125
- flow rate should be 75.7 lpm
- as with all emergency washes, the water provide should be tepid (16-30 oC) for at least 15 minutes
FIRST AID
Laboratory Safety CSMLS Guidelines 9th ed pages 100-101; 8th ed pages 129-131
- being prepared for appropriate first aid requires:
- training for an appropriate number of personnel (a minimum number is needed per shift)
- ready access to first aid supplies, on all shifts
- established procedures for follow-up medical assistance – there will be no time when it’s needed
- emergency contact information for seeking assistance
- first aid kits must be available at all times
Center, D. (2018, June 12). Did you know #prep and #PEP are two effective #HIV prevention methods? for more
info, visit https://t.co/n2snothotu . PIC.TWITTER.COM/4GDWEWL3YY. Twitter. Retrieved April 30, 2023, from
https://twitter.com/DamienCenter/status/1006546941717766145
MEDICAL EMERGENCIES
- good first aid training helps determine what is needed
- automatic external defibrillators (AED) pus the heart in normal rhythm
References:
ANSI/ISEA Z358.1 Resource Center. Haws Co. (2022, December 16). Retrieved April 30, 2023, from https://www.hawsco.com/support/ansi-z358-1-resource-
center
Ccohs. (2019, September 23). Share this #infographic outlining the main elements of an emergency preparedness plan and the key steps to
#emergencyplanning, because everyone in your workplace needs to be ready for the sudden or unexpected https://t.co/ygktlrv5yi
PIC.TWITTER.COM/FIPL9TJZ6G. Twitter. Retrieved April 30, 2023, from https://twitter.com/CCOHS/status/1176120306710302720
Laser light amplification. (n.d.). Retrieved April 30, 2023, from https://www.zbz-7.top/products.aspx?cname=laser%2Blight%2Bamplification&cid=42
Center, D. (2018, June 12). Did you know #prep and #PEP are two effective #HIV prevention methods? for more info, visit https://t.co/n2snothotu .
PIC.TWITTER.COM/4GDWEWL3YY. Twitter. Retrieved April 30, 2023, from https://twitter.com/DamienCenter/status/1006546941717766145
O’Grady, E. (2022). Laboratory Safety CSMLS Guidelines (9th ed.). CSMLS Canadian Society for Medical Laboratory Science.
Price, T. (n.d.). Nonionizing radiation (NIR) overview - ppt download. SlidePlayer. Retrieved April 30, 2023, from https://slideplayer.com/slide/4889506/
Shematek, G., Wood, W., O'Grady, E., & Canadian Society for Medical Laboratory Sciences. (2017). Laboratory safety CSMLS guidelines
UC Santa Cruz Laser Safety training - ppt download. SlidePlayer. (n.d.). Retrieved April 30, 2023, from http://slideplayer.com/slide/1676012/
41
ASSIGNMENT 5
The next questions use the following as the answer key:
a. ionizing radiation b. non-ionizing radiation
12. 13.
The next questions refer to classes of lasers on page 66 in the CSMLS 9th Ed; page 91 in the 8th Ed and
use the following as the answer key:
a. I b. II c. IIIA d. IIIB e. IV
19. Eyes are most susceptible to damage by lasers, and the skin can also be damaged by burns.
20. Incidental exposure to a Class 4 laser will not cause serious skin burns or retinal damage.
21. Sources of reflection for laser light include mirrors, jewelry, and glossy surfaces.
22. The main hazard associated with microwave is radiation exposure.
23. Metal reflects microwaves and should not be used in a microwave.
24. All microwave ovens are dangerous to pacemakers.
25. When heating reagents in a microwave oven, ensure the containers are only lightly covered to
prevent pressure build-up.
26. Ultraviolet light in the lab is primarily a skin hazard.
27. Do not use UV lights while performing work in the laboratory.
28. UV light can be reflect from surfaces 29. Turn UV lights off when not in use.
42
Identify the following pictograms
https://en.wikipedia.org/wiki/Laser_safety and https://www.uaf.edu/safety/industrial-hygiene/laboratory-safety/physical-hazards.php and https://www.labelsource.co.uk/labels/non-ionizing-radiation-symbol-safety-sign-/ws3530
and https://shop.blairsigns.com.au/High-Noise-Area/Hearing-Protection-Must-Be-Worn-370
a. b. c. d. e. f. g. h.
42. An emergency plan is a response to an event which has occurred in the past.
43. Incident reporting and investigation is about fault finding.
44. Hazards that may require drenching or flushing include all if the following EXCEPT:
a. corrosive or toxic chemicals
b. exposure to lasers or UV light
c. open source nuclear substances
d. infectious substances
e. dust or projectiles
45. 16-30 OC water should be used 46. should be used for a min of 15 min
47. check weekly and document 48. inspected annually (document)
49. 50.
43
The next questions use the following as the answer key:
7460B Axion MSRTM - PNR. (n.d.-a). https://www.pnr.co.uk/media/2016/03/7460B_Axion_eye-face_wash_wall_mounted_stainless_bowl.pdf and Global IndustrialTM emergency Eyewash Station, wall mount. (n.d.). https://www.globalindustrial.ca/p/global-emergency-eyewash-wall-
mounted-plastic-bowl
51. activated by pulling the chain 52. flow rate should be 1.5 lpm
53. flow rate should be 11.4 lpm 54. flow rate should be 75.7 lpm
55. 56.
57. Emergency was devices must meet the ANSI Z358.1-2014 standard.
58. should provide a source of cold running water
59. water provided should be in contact skin or eyes for 15 minutes
60. Tepid water has a temperature between 16 and 38oC.
61. After checking the operation of a safety shower or eye wash, you should record the date and
results of the test.
62. The emergency wash should be tested monthly, at a minimum.
63. The emergency shower is the most commonly used emergency wash device.
64. Emergency wash stations must be within a 10-second walk of the potential hazard.
65. It is okay for the emergency wash station to be located up one stair from the hazard.
66. Storage of bulky items beneath a shower is acceptable as long as the items can be quickly
moved in the event the shower is needed.
67. If the emergency wash device is bright orange, there does not need to be signage.
68. When a portable eyewash is used, the contents should be verified weekly.
69. The organ most sensitive to corrosives is the skin. (the eyes)
70. The eyewash is the most critical emergency wash device.
71. What should you immediately do if you splatter a hazardous chemical on you?
a. go home and take a shower
b. continue to work on your research
c. use the emergency shower to promptly wash off the chemical
d. call ambulance
73. If an individual's skin is splashed with a corrosive acid, neutralizing sodium bicarbonate
should be applied to the affected area.
74. A privacy curtain around an emergency drench shower is optional, but highly recommended.
75. Remove clothing before using the emergency shower.
44
The next questions use the following as the answer key:
a. true b. false
76. When using an emergency eye wash, eyelids should be held open with thumb and forefingers
so that water can totally flush the eye.
77. Contacts should be removed before using an emergency eye wash.
78. The main problem in using a handheld drench hose for eye washing is that both hands are not
free for manipulating the injured eye(s).
79. When a safety shower drains directly into a sewer, you need to determine whether residual
water can contaminate the outside environment.
80. What should you do if a chemical spill poses an immediate risk to you and your co-workers?
a. mark it with a sign or barricade
b. clean it up immediately
c. notify staff and evacuate the area
81. The number of first responders legally required depends upon the number of personnel
employed.
82. First aid medical follow-up procedures should be documented and communicated.
83. First aid supplies should be kept locked in a secure location to prevent theft.
84. Seek professional medical assistance after you perform first aid.
85. If acid is spilled on a person, remove their clothing as you proceed to the shower.
86. If a corrosive chemical has been completely washed off a victim, and he or she is feeling well,
there is no need to see medical attention.
87. After a spill involving the eye, the victim was taken to the eyewash. After one minute, her
contacts were removed. She should continue to irrigate her eyes for a further 14 minutes.
88. Blisters from burns should be drained immediately, to facilitate the antibiotic cream.
89. In the event of a burn, do not force clothing off a burn if it does not come off easily.
90. Place ice directly on a burn to prevent damage to deeper layers of tissue.
91. Do not put butter or other oils on a burn.
92. In the event of a clothing fire, extinguish the flames with a fire blanket.
93. The identity of a chemical involved in a chemical poisoning should be provided to emergency
medical personnel.
94. Always induce vomiting when poisoning is expected.
95. When a person is the victim of an electrical shock, unplug the appliance immediately.
96. In the event of an electrical shock, seek medical assistance even if it doesn’t look bad.
97. In the event of a cut, apply a moist bandage.
98. Elevate a cut above head level to stop the bleeding.
99. Punctures caused by a needlestick should be squeezed to make them bleed.
100. As soon as a needle stick occurs, seek medical treatment immediately.
101. Post exposure prophylaxis after a needlestick greatly decreases the risk of contracting HIV.
102. All needlesticks must be reported.
103. First aid kits must be accessible at all times
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104. When a person’s clothing is on fire, they should:
a. stop, drop, roll
b. pull, aim, squeeze, sweep,
c. contain, absorb, clean
d. eat, pray, love
e. wash, rinse, repeat
105. Typically, the best course of action to take during a medical emergency is to:
a. begin first aid immediately
b. activate the emergency plan for reporting injuries
c. notify the person's family about the situation
106. You think you may have had an exposure, but are unsure. You check your glove for punctures and find
that it is broken. You should assume:
a. an exposure has occurred
b. no exposure has occurred
c. you can continue working; you’re probably fine
d. no blood, no harm
107. Which of the following are recommended practices to reduce risk of needlestick injuries?
a. never retrieve anything from a sharps container
b. never recap a used needle
c. use safety engineered needles (retractable or with needle covers)
d. do not overfill the sharps container
e. all of the above
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LABORATORY ERGONOMICS
Laboratory Safety CSMLS Guidelines 9th ed pages 69-74; 8th ed pages 94-101
- ergonomics is the adjustment of the workspace to fit the employee
- applies to workstation design, posture, breaks, equipment and furniture design, material handling etc.
- good ergonomics increases efficiency and comfort
- poor ergonomics can lead to musculoskeletal disorders (MSDs) and injuries (MSIs)
MSDs /MSIs
- symptoms include muscle fatigue, bands of pain, pain/stiffness when changing positions, difficulty in
hand movement, loss of sensitivity, numbness and tingling
- often occurs in the neck, back, shoulders, elbows, wrist, fingers and knees
- risk increased by awkward postures/ movements, repetitive movements and amount of force applied
- other signs of poor ergonomics include fatigue, repetitive motion injuries, monotony (leads to increased errors
and accident rates, eye strain and lowered morale)
adapted from: Practical demonstrations of Ergonomic Principles - CDC. (n.d.). Retrieved May 2, 2023, from https://www.cdc.gov/niosh/mining//UserFiles/works/pdfs/2011-191.pdf
- use adjustable laboratory benches, because different types of work required different desk heights
- design lab space to avoid awkward movements like reaching
- chairs should be adjustable, with casters, 5 legs, and a backrest
- remember to adjust you chair and use good posture
- floors should be slip-resistant, especially in Pathology (uses wax)
- use anti-fatigue mats when standing for prolonged periods
- allow for frequent short (a few min) breaks from repetitive work
- vary tasks and scheduling so you can change positions and use different muscles
- control the use of vibration-producing equipment like vortex mixers which are hard on muscles
- use automatic pump-type pipettors which lessen stress on the thumb
- be aware of accessibility and size of knobs and buttons on equipment
- use of automated foot operated equipment when possible
- use hand tools and equipment designed for easy manipulation
- ask for assistance when moving heavy OR light but awkward items
- store heavy materials on lower shelves
- use anti-glare video displays/monitors at eye level or slightly lower
Jason. (2016, January 29). Ergonomics at the office. 404 Tech Support. Retrieved
May 2, 2023, from https://www.404techsupport.com/2009/06/24/ergonomics-at-
the-office/
- monitor indoor air quality (temperature, humidity, fresh air) and keep noise low
- a task analysis is a good way to find ergonomic problems
- ergonomic considerations should be part of purchasing and include flexibility for different body types
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The CSMLS Safety Guidelines has more information on ergonomics for specific tasks on pages 70-71 of
the 9th Ed; pages 95-98 in the 8th Ed. Consult the Guidelines as you actually do the tasks in the lab
Pages 72-74 in the 9th Ed; pages 99-101 in the 8th Ed have some good ergonomic exercises
PSYCHOLOGICAL HAZARDS
Laboratory Safety CSMLS Guidelines 9th ed pages 75-76; 8th ed pages 102-107
- physical and mental illnesses can be negatively affected by excessive psychological stress
- it is important to recognize the sigs of excessive stress so you can try to reduce that stress
- physical symptoms of stress include muscle tension, insomnia, loss of appetite, dry throat and mouth,
trembling or twitches, headaches, high blood pressure, cold, clammy hands
- behavioural manifestations of stress include restlessness, irritability, excessive smoking, drinking or drug
use, withdrawal from others, indecisiveness, impulsive behavior
- mental and emotional symptoms of stress include lack of concentration, forgetfulness, preoccupation, mood
swings, hostility, brooding and worrying, daydreaming, anxiety, fear
CAUSES OF WORKPLACE STRESS
- work organizational factors include issue with others, like violence and abuse, harassment or conflict
- organizational factors may involve just you, like working alone, critical incident stress (like seeing someone
die), change and uncertainty, fatigue and hours of work (often happens with staff shortages)
- environmental factors include noise (from instrumentation, phones, people) and indoor air quality and lighting
- personal factors include substance abuse, depression / mental illness, age-related factors, work-life conflict
- the laboratory workplace has particular stresses:
- “production line” type work (load instrument, unload instrument, check computer, repeat)
- overtime work, or the need to work faster and faster
- urgency of STAT tests
- risk of harmful exposures (chemical, biological, physical)
- ethics of confidentiality (at home AND work, hard to know something not tell)
- emotional stress from very ill patients (empathy is often good, but can be very hard on you)
- making errors that could have serious impact (what if your error killed someone?)
- job insecurity (especially when casual or temporary)
- poor management-employee relations
CONTROLLING AND REDUCING STRESS
- many different ways, depending on what the stress is
- communication and honest feedback, a fair workload and employee assistance programs are important
- easing stress from environmental factors includes communication of construction activities, follow-up of
complaints and controls for noise levels etc
- ways to manage personal stress include self-renewal activities, reduction of body metabolism and good
general health practices, counselling, prescribed treatment
ABUSE/VIOLENCE
- now treated and a health and safety issue and many provinces have specific legislation for these issues
- includes bullying, verbal and sexual harassment, physical and sexual assault, intimidation and threats
- early intervention is important to prevent escalation of disruptive behavior
- like all safety issues, there should be training in recognizing workplace violence, policies and procedures to
eliminate workplace violence, an appropriate response and a reporting and investigating system
BULLYING
- a common and subtle form of abuse
- workplace bullying is repeated mistreatment of employees by employees that endangers health or career
- many kinds of bullying behaviors
WORKING ALONE
- legislation related to working alone was developed as a result of accidents or violence
- controls for working alone include:
- identify workers who work may be required to work alone
- have an emergency communication plan so others can be alerted for assistance
- control access to the lab
- schedule to prevent working alone
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TECHNOSTRESS
- technology changes fast, and we must keep up
- stressful to have to learn a new technology when equipment fails or you have to learn new equipment
- equipment needs to be as user-friendly as possible, with great tech support
- administration needs to have realistic expectations
- iindividuals need to self educate and communicate about stresses
REPRODUCTIVE HAZARDS
Laboratory Safety CSMLS Guidelines 9th ed pages 77-80; 8th ed pages 108-112
- reproductive risks are any agents that can impact the ability to have healthy children
- affects males and females
- effect people differently, depending on exposure (when, how and length)
- outcomes of pregnancy are mainly not work related
- information based on animal studies, which does not always translate to people
- many effect people not animals, animals not people
- chemicals can be found in breast milk, so nursing mothers have a higher risk
References:
Alberta Government. (2017). A health care worker’s guide to health and safety on the job. Retrieved May 2, 2023, from https://open.alberta.ca/dataset/a4509e0f-
3c7d-45a3-bd2a-f63efb2a81ad/resource/389451f8-5213-42a0-b28c-17a564684511/download/ohs-best-practices-bp023.pdf
Calm clinic - information about anxiety, Stress & Panic. Calm Clinic - Information about Anxiety, Stress and Panic. (n.d.). Retrieved May 2, 2023, from
https://www.calmclinic.com/
Centers for Disease Control and Prevention. (2023, May 1). Pregnancy and your job - reproductive health. Centers for Disease Control and Prevention.
Retrieved May 2, 2023, from https://www.cdc.gov/niosh/topics/repro/pregnancyjob.html
Decommissioning labs.pptx. Share and Discover Knowledge on SlideShare. (n.d.). Retrieved May 2, 2023, from
https://www.slideshare.net/ACSDCHAS/decommissioning-labspptx
Ergonomics: Best practices lifting tips and techniques - PPT video online download. SlidePlayer. (n.d.). Retrieved April 8, 2022, from
http://slideplayer.com/slide/1456373/
Jason. (2016, January 29). Ergonomics at the office. 404 Tech Support. Retrieved May 2, 2023, from https://www.404techsupport.com/2009/06/24/ergonomics-
at-the-office/
Kerkes, D. (n.d.). Repetitive strain injury and 4 ways to prevent it. Repetitive Strain Injury And 4 Ways To Prevent It. Retrieved May 2, 2023, from
https://www.hallam-ics.com/blog/repetitive-strain-injury-and-4-ways-to-prevent-it
Laboratory Ergonomics Occupational Health & Safety Department of Environmental Health & Safety Washington State University. - ppt download. SlidePlayer.
(n.d.). Retrieved April 9, 2022, from http://slideplayer.com/slide/1573353
(n.d.). Retrieved from: http://labs21.lbl.gov/DPM/Assets/ITcX.pdf
O’Grady, E. (2022). Laboratory Safety CSMLS Guidelines (9th ed.). CSMLS Canadian Society for Medical Laboratory Science.
Practical demonstrations of Ergonomic Principles - CDC. (n.d.). Retrieved May 2, 2023, from https://www.cdc.gov/niosh/mining//UserFiles/works/pdfs/2011-
191.pdf
Sachs, & Spina. (n.d.). Birth defects 3.01 revised 10/19/10 K.Brown. - ppt download. SlidePlayer. Retrieved May 2, 2023, from
http://slideplayer.com/slide/4734107
Shematek, G., Wood, W., O'Grady, E., & Canadian Society for Medical Laboratory Sciences. (2017). Laboratory safety CSMLS guidelines
SitFlow. (n.d.). Repetitive strain injuries in the workplace. SitFlow. Retrieved April 9, 2022, from https://sitflow.com/blogs/news/repetitive-strain-injuries-in-the-
workplace
Toxicology testing in animals narrated part 12013. Share and Discover Knowledge on SlideShare. (n.d.). Retrieved May 2, 2023, from
https://www.slideshare.net/BethConover/toxicology-testing-in-animals-narrated-part-12013
Workplace violene threat/interaction/response leadership review - ppt download. SlidePlayer. (n.d.). Retrieved May 2, 2023, from
https://slideplayer.com/slide/3552997/
ASSIGNMENT 6
The next questions use the following as the answer key:
a. true b. false
19. Some conditions that can cause musculoskeletal disorders can be brought on by:
a. sudden increase in your workload
b. introduction of a new process to your work routine
c. use of vibrating tools
d. maintaining poor or unhealthy posture
e. all the above
20. Indicate (X) which of the following show GOOD ergonomic technique.
Bakker Elkhuizen Ergo-Q 260 notebook stand: Applied ergonomics chicago. Applied Ergonomics. (2023, April 23). Retrieved May 2, 2023, from https://www.appliedergonomics.com/product/bakker-elkhuizen-ergo-q-260-notebook-stand/ and Free Ergonomics Workshop at
grassroots juicery. DeKalb County Online. (2020, October 12). Retrieved May 2, 2023, from https://dekalbcountyonline.com/2015/10/free-ergonomics-workshop-at-grassroots-juicery/ and (n.d.). Retrieved from: http://www.flexibility.co.uk/images/09/300s/ergonomics-bakker3diag-
300.jpg/
21. Using alcohol, drugs or cigarettes to cope is a sign of excessive workplace stress.
22. Stress has an impact on the quality of your relationships and how you interact with others.
23. Eating alone on the job at lunchtime is healthier for you than getting away from your
workstation and having lunch.
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The next questions use the following as the answer key:
a. true b. false
24. Developing friendships with some of your co-workers is a waste of time - you're there to work
and nothing else.
25. As you learn to manage your job stress, you'll have more control over your ability to think
clearly and act appropriately.
26. Stress prevention should be tackled by workers and employers together.
27. Exercise uses up your energy and leaves you feeling more tired at work.
28. Working long hours can lead to increased work stress.
29. Employers cannot make any changes to workers' duties if workers are stressed on the job.
30. "Deep breathing" is a relaxation technique that can help you to reduce stress.
31. Studies show that being in good physical shape has nothing to do with stress levels.
32. You have a legal right to a safe working environment.
33. A healthy diet, adequate sleep, and regular exercise can help you deal with on-the-job stress.
34. If you feel a lack of control over your job, your only choices are putting up with it or quitting.
35. Workplace disruptive behaviors are a health and safety issue.
36. There is usually no warning in cases of workplace violence.
37. There should be zero-tolerance for workplace abuse.
38. All bullies are same.
39. Incidents of bullying should be documented in detail.
40. Even if you like working alone, it is still a hazard.
41. If you use your phone a lot, you are unlikely to have workplace technostress.
42. Making the lab less automated is a good way to reduce technostress.
43. What is the name of the condition where problems grow to unmanageable levels?
a. stress fracture
b. adrenaline overload
c. stress overload
45. Which of these is the most effective long-term solution to on-the-job stress?
a. relaxation exercises
b. effective time management
c. worker-friendly organizational changes
d. vacations
59. Thalidomide affects a developing child in utero, causing missing limbs, ears, or death. Surviving victims
of thalidomide are at no greater risk of having a baby with birth defects than the general population.
How should this drug be classified?
a. mutagen
b. teratogen
c. mutagen and teratogen
60. teratogen
61. can cause deafness, blindness, learning disabilities and miscarriages
62. can cause heart disease, blindness and deafness within the first three months
63. can cause physical difficulties, arm or leg defects and death
78. ensuring all building systems perform according to the design intent
79. ensuring a facility has no physical, chemical, biological or nuclear hazards
80. you bought a new biosafety cabinet and you must have it installed and certified
81. your chemistry analyzer module is malfunctioning and you have to get it ready to send back
to the manufacturer