Results
Absorbance
Using the formula: Percentage hemolysis=
Absorbance of Tube 8∈table 1
Percentage Percentage Percentage
Absorbance Absorbance Absorbance
TESTUBES hemolysis hemolysis hemolysis
(NACL) (glucose) (urea)
(NACL) (glucose) (urea)
1 0.09 0.88 0.57 7.692 75.214 48.718
2 0.09 0.95 0.61 7.692 81.196 52.138
3 0.09 0.84 1.03 7.692 71.794 88.034
4 0.19 0.98 1.48 16.239 83.760 126.496
5 0.42 0.98 1.63 35.897 83.760 139.316
6 0.86 0.96 1.29 73.504 82.051 110.256
7 0.8 1.11 0.63 68.376 94.871 53.846
8 1.17 1.08 0.62 100 92.307 52.991
A graph of showing the effect of molar concentration of
glucose on percentage hemolysis
100
90
80
70
Percentage Hemolysis
60
50
40
30
20
10
0
0.04 0.06 0.08 0.1 0.12 0.14 0.16
Molar Concentration of Glucose
A graph showing the effect of the concentration of NaCl on
Percentage hemolysis
120
100
percentage hemolysis
80
60
40
20
0
0 0.02 0.04 0.06 0.08 0.1 0.12
Molar concentration of NaCl
A graph showing the effect of molar concentration of Urea on
Percentage hemolysis
160
140
120
100
Percentage
80
60
40
20
0
0.15 0.125 0.112 0.1 0.087 0.075 0.062 0.05
Molar concentration of Urea
CHANGE IN CELL VOLUME=VOLUME OF CELLS AFTER SECOND CENTRIFUGATION-VOLUME OF CELLS
AFTER INITIAL CENTRIFUGATION
Test tubes Volume of
Volume of cells
with varying cells after Changes in cell
after initial
concentrations second time volume
centrifugation
of (NACL) ,% centrifugation
1.25 0.5 0.4 -0.1
0.9 0.5 0.5 0
0.7 0.5 0.7 0.2
0.5 0.5 0.9 0.4
A graph of Changes in cell volume against Sodium chloride
concentrations.
0.5
0.4
0.3
Changes in cell volume
0.2
0.1
0
0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3
-0.1
-0.2
