USER GUIDE

ProFlex™ PCR System User Guide

Installation, Use, and Maintenance
for use with:Other Real-Time PCR Systems

Catalog Number 4484075 (ProFlex™ 96-Well PCR System), 4484073 (ProFlex™ 3x32-Well PCR Sys-
tem), 4483637 (ProFlex™ 96-Well Sample Block), 4483638 (ProFlex™ 3x32-Well Sample Block))
Publication Number MAN0007697
Revision 01

For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.
The information in this guide is subject to change without notice.

DISCLAIMER
LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO
THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT,
WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL
DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.

Placeholder for other notices

TRADEMARKS
The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners.
© 2013 Life Technologies Corporation. All rights reserved.
 Contents

About this Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Revision History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Pre-requisites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

■ CHAPTER 1 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

About the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
™
ProFlex Touchscreen menu overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
™
Using the ProFlex System Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Entering text (ICONS and screenshot to be updated in next draft) . . . . . . . . . . . . . . . . 15
Entering numerals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
™
Consumables for the ProFlex 96-Well PCR System . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
™
Consumables for ProFlex 3x32-Well PCR System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

■ CHAPTER 2 Instrument Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Site requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
System specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Environmental requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Temperature and humidity requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Pollution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Altitude . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Required materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Optional protective hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
™
Unpacking the ProFlex System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
™
Setting up the ProFlex System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
™
How to set up the wireless connection in the ProFlex System . . . . . . . . . . . . . . . . . . . . . . . 24
Changing sample blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Connecting to a network printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Connecting together multiple instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
You will need . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Making the connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

ProFlex™ PCR System User Guide 3

For Research Use Only. Not for use in diagnostic procedures.
Contents

™
Setting up the ProFlex System touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Recommended instrument settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Adding users (screenshots to be updated) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Setting other options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Setting idle temperatures for the heated cover (PLEASE CONFIRM STEPS 2
THROUGH 4) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Setting time-outs (NEED NEW CONTENT) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

■ CHAPTER 3 Running PCR Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Loading samples into the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
™
Loading samples into the ProFlex 96-Well PCR System . . . . . . . . . . . . . . . . . . . . . . . 34
™
Loading samples in the ProFlex 3x32-Well PCR System . . . . . . . . . . . . . . . . . . . . . . . 35
Creating a new run method (PLEASE CONFIRM CONTENT. SCREENSHOTS TO BE UP-
DATED IN NEXT DRAFT) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Performing a run using an existing method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Editing details (content and screenshots to be updated) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Adding a stage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Adding a step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Editing temperature and time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Editing ramp rates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Creating an AutoDelta step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Deleting a stage or step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
™
Creating a VeriFlex step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Performing a run (please confirm content) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
........................................................................ 0
Monitoring a run (please confirm content) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Monitoring via the Home screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Monitoring via the run monitor screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
If the power fails . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Viewing and printing the run report (PLEASE CONFIRM CONTENT) (NEED SCREEN-
SHOTS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Removing the samples from the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

■ ™
ProFlex 3x32 Well PCR System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

■ ™
ProFlex 1x96 Well PCR System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

■ CHAPTER 4 Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Scheduled maintenance (SECTION TO BE DELETED?? ) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
As-needed maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Cleaning the instrument (NEED SOME CONCEPTUAL CONTENT HERE) . . . . . . . . . . . 56
Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Ordering kits and replacement parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

4 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Contents

■ CHAPTER 5 Utilities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Simulation modes (screen are WIP. Section to be updated in next draft) . . . . . . . . . . . . . . . 60
Upgrading system firmware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
About the upgrade . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
™
Upgrading the ProFlex PCR System firmware (PLEASE CONFIRM STEP 3) . . . . . . . 60
™
Viewing ProFlex Instrument statistics (PLEASE CONFIRM CONTENT) . . . . . . . . . . . . . . . . 61
Restoring factory settings (PLEASE CONFIRM CONTENT) . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

■ APPENDIX A Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

■ APPENDIX B Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Troubleshooting problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Returning an instrument for service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

■ APPENDIX C Instrument specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

System specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Location of power point and ports on the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

■ APPENDIX D Predefined Run Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

®
AmpliTaq Gold Fast PCR run method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
®
BigDye Kit Fast run method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
®
BigDye Kit Standard Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
General PCR run method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Time Release run method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Touchdown PCR run method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

■ APPENDIX E Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Symbols on this instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Conformity symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Safety alerts on this instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Location of safety labels on this instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Safety information for instruments not manufactured by Life Technologies . . . . . . . . . . . 79
Instrument safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
z_Physical Injury_ProFlex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Cleaning and decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

ProFlex™ PCR System User Guide 5

For Research Use Only. Not for use in diagnostic procedures.
Contents

Safety and electromagnetic compatibility (EMC) standards . . . . . . . . . . . . . . . . . . . . . . . . . . 80

Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
EMC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Environmental design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Chemical safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Biological hazard safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

■ Documentation and Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

Related documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Obtaining SDSs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Obtaining Certificates of Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Obtaining support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Limited product warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

6 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 About this Guide

Revision History
Revision Date Description

01 April 2013 New document

Purpose
The ProFlex™ PCR System User Guide provides information about installing, using,
and maintaining the ProFlex™ PCR System.

Pre-requisites
This user guide is intended for personnel who have been specifically trained by Life
Technologies. The manufacturer is not liable for damage or injury that results from
use of this manual by unauthorized or untrained parties.

ProFlex™ PCR System User Guide 7

For Research Use Only. Not for use in diagnostic procedures.
 1 Product Overview

The ProFlex™ PCR System is an automated end-point thermocycling system, specifi-

cally designed for the amplification of nucleic acids using the Polymerase Chain Reac-
tion (PCR) process. The user interface consists of a Touchscreen with a graphical dis-
play screen that shows the time, status, and temperature profile for each run. A
touchscreen keypad allows you to enter information into fields on the display screen.

About the system

There are two models of the ProFlex™ PCR System:
• ProFlex™ 96-Well PCR System
• ProFlex™ 3x32-Well PCR System
You control the ProFlex™ PCR System using the touchscreen display and easy-to-use
interface. You can connect more than 12 ProFlex™ PCR Systems in a satellite-like for-
mat through a network switch. With this connection, you can transmit run methods
from one ProFlex™ PCR System to another and start multiple thermal cyclers at once.
The ProFlex™ PCR System has a run recovery feature. If a power failure occurs during
a run, the instrument will attempt to continue the run when the power returns. In ad-
dition, the ProFlex™ 96-Well PCR System and the ProFlex™ 3x32-Well PCR System in-
clude the VeriFlex™ Blocks. In the ProFlex™ 96-Well PCR System, the VeriFlex™ Blocks
are six independently regulated thermal blocks that aid in designing a primer set and
run method for optimal PCR conditions. The ProFlex™ 3x32-Well PCR System in-
cludes two thermal blocks.

Figure 1 ProFlex™ 3x32-Well PCR System

8 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Chapter 1 Product Overview
ProFlex™ Touchscreen menu overview 1

Figure 2 ProFlex™ 96-Well PCR System

This user guide provides information on how to maximize the capabilities of your
ProFlex™ PCR System. This user guide provides instructions for both the models of
the ProFlex™PCR System. Functions which are not the same for each model are indi-
cated by notes at the beginning of the section describing the function.
Detailed specifications for the ProFlex™ PCR System are found in Appendix C, “In-
strument specifications”.

ProFlex™ Touchscreen menu overview

On powering on the ProFlex™ PCR System, you will first see the following Splash
screen.

ProFlex™ PCR System User Guide 9

For Research Use Only. Not for use in diagnostic procedures.
1 Chapter 1 Product Overview
ProFlex™ Touchscreen menu overview

Figure 3 ProFlex™ PCR System Splash screen

Shortly, the Home screen appears. Since the ProFlex™ PCR System has interchangea-
ble blocks, the current block is initializing when the Touchscreen displays the Home
screen. After the block completes initializing, depending the block in use, the Home
screen displays those many dials; three for the ProFlex™ 3x32-Well Sample Block and
one for the ProFlex™ 1x96-Well Sample Block.

10 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Chapter 1 Product Overview
ProFlex™ Touchscreen menu overview 1

Figure 4 ProFlex™ PCR System Home screen

The Home screen includes the following features:

• Dial: The number of dials appearing on the Home screen varies with the block in
use. The dial displays the current temperature of the block, the time elapsed for a
run, and the run status.
On initial install, the blocks (one in case of the ProFlex™ 96-Well PCR System and
three in the ProFlex™ 3x32-Well PCR System) will show the 'Set Up Run' display.
You can start a run by touching Set Up Run. For instructions on starting a run by
this method, see (insert link later).
• My Account: Touch My Account to enter or edit the account information.
• New Method: Touch New Method to create a new run method with the help of
the default templates that are pre-loaded with the ProFlex™ System (screenshot
not available at the moment; insert later).
• Open Method: Touch Open Method to select an existing run method to start a
run. Touching Open Method takes you to the Select Method screen that displays
the existing run methods. The run methods are sorted and stored in various fold-
ers. Select a folder to display the run methods within the folder. Touch a run
method under the Method name to edit that run method in the edit mode.

ProFlex™ PCR System User Guide 11

For Research Use Only. Not for use in diagnostic procedures.
1 Chapter 1 Product Overview
ProFlex™ Touchscreen menu overview

Figure 5 ProFlex™ PCR System Select Method screen (to be updated)

• Incubate: Touch Incubate to use the ProFlex™ instrument as a refrigerator or
heater.

• Settings: Touch Settings to configure the ProFlex™ Instrument (entire Settings

section is incomplete... to be updated in next draft).

12 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Chapter 1 Product Overview
ProFlex™ Touchscreen menu overview 1

You can configure the following from the Settings screen:

– About Instrument
– Device Settings
– Run History
– Transfer Methods
– Administrator Settings
– Maintenance & Service
Touch Device Settings to set various additional parameters:

You can set the following in the Device Settings screen:

– Instrument Name
– Date/ Time

ProFlex™ PCR System User Guide 13

For Research Use Only. Not for use in diagnostic procedures.
1 Chapter 1 Product Overview
Using the ProFlex™ System Touchscreen

– Sleep Mode
– Network Connection: Touch Network Connection to select the type of net-
work connection. You can select from the Wireless and Ethernet options. For
details on using the Wireless and Ethernet options, see (insert link later).

– Multiple Device Setup

– Restore Factory Settings
– Heated Cover

Using the ProFlex™ System Touchscreen

You interact with the ProFlex™ System using a touchscreen. Table 1 describes the sev-
eral buttons common to many of the screens in the ProFlex™ System.

Table 1 Buttons in the ProFlex™ System touchscreen

Button Function

Returns to the previous screen

When a run is in progress, displays the run

status.

14 ProFlex™ PCR System User Guide

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 Chapter 1 Product Overview
Using the ProFlex™ System Touchscreen 1

Button Function

Scrolls up and down through a list of items.

Indicates that a USB drive is connected to

the instrument’s USB port.

Closes the current modal window.

Indicates whether the Wi-Fi is on or off.

Entering text When you touch a field that requires the input of text, the text editor, as seen in the
(ICONS and following figure, opens. Table 2 displays the buttons to touch to enter different infor-
mation types.
screenshot to be
updated in next screenshot of keypad to be added
draft)
Table 2 Buttons to enter different information

To... Touch

Enter a lowercase letter A letter button.

Enter an uppercase letter Insert graphic of button. Caps, then the letter (s). When
you are done, touch Caps again.

Enter punctuation or other Insert graphic of button. Symbols, then the symbol(s).
symbols When you are done, touch Symbols again.

Delete the last letter Insert graphic of button.

Close the editor and save any Insert graphic of button.

changes

Close the editor and discard Insert graphic of button.

any changes

Entering When you touch a field that requires a numerical input, the numeric editor, as seen in
numerals the following figure, opens. Table 3 displays the buttons to touch to perform different
functions while entering numbers.

ProFlex™ PCR System User Guide 15

For Research Use Only. Not for use in diagnostic procedures.
1 Chapter 1 Product Overview
Consumables

Table 3 Functions performed by entering numbers

To... Touch

Enter a number A number button.

Enter a decimal , then the remaining digit(s).

Enter slash Insert graphic of button. /, then the remaining digit(s). (to
be added in next draft)

Delete the last number

Close the editor and save any

changes

Consumables
Use this section to determine the tube, sample tray, and plate configurations appro-
priate for your ProFlex™ System.
• Consumables for ProFlex™ 96-Well PCR System
• Consumables for ProFlex™ 3X32-Well PCR System

16 ProFlex™ PCR System User Guide

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 Chapter 1 Product Overview
Consumables 1

Consumables for 96-Well sample tray and plate configurations

the ProFlex™96- The following table lists the possible sample tray and plate configurations.
Well PCR System
IMPORTANT! The MicroAmp™ Splash Free 96-Well Base shown below is used only
when pipetting samples into tubes or trays. Do not place it in the sample block(s).

Consumable Compatible component

MicroAmp™ MicroAmp™ Caps, 8-Cap Strip

Optical 96-
or
Well Reac-
™
tion Plate MicroAmp Clear Adhesive Film (not
(0.2mL) shown)

MicroAmp® 96-Well Tray for VeriFlex™ Blocks

Reaction
Tube with
Cap

Micro- MicroAmp™ 8-Cap Strip

Amp™8-
and
Tube Strip
™
MicroAmp 96-Well Tray/ Retainer
or
Set for ProFlex™ Systems
MicroAmp®
Reaction
Tube with-
out Cap

96-Well tube configurations

IMPORTANT! The tray or the tray/retainer is required for the operation of the Pro-
Flex™ System.

You can use three different types of tube configurations to hold your samples, as
shown in the following figures.

ProFlex™ PCR System User Guide 17

For Research Use Only. Not for use in diagnostic procedures.
1 Chapter 1 Product Overview
Consumables

™
Figure 6 Different tube configurations for use in the ProFlex 96-Well PCR System

Consumables for The ProFlex™ 3x32-Well PCR System does not use reaction plates. Instead, Micro-
ProFlex™ 3x32- Amp® Reaction Tubes and MicroAmp® Tube Strips are used. The MicroAmp® reac-
tion tubes/ tube strips are placed in a MicroAmp® 3x32-Well Retainer and the retainer
Well PCR System
is then placed on the block. The following figure (placeholder photo to be replaced by
a line drawing) displays the ProFlex™ 3x32-Well Sample block.

18 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Chapter 1 Product Overview
Consumables 1

ProFlex™ PCR System User Guide 19

For Research Use Only. Not for use in diagnostic procedures.
 2 Instrument Setup

This chapter includes the procedures for installing the ProFlex™PCR System.

Site requirements
The ProFlex™ PCR System is for indoor use. Ensure that the installation site:
• Meets the spatial and weight requirements (see “System Specifications” -- insert
link later)
• Meets environmental requirements (see “Environmental Requirements” -- insert
link later)
• Is within 1 m (3 ft.) of a 800 VA power receptacle
• Is away from water

System specifications
Table 4 Physical dimensions, weight, and power consumption

Parameter Instrument foot- Recommended clear- Total

print ance

Height 27.2 cm (10.7 in) 15.5 cm (6.1 in)[1] 42.5 cm (16.7 in)

Width 33.0 cm (13 in) 0 cm (0 in) 33.0 cm (13 in)

Depth 56.5 cm (22 in) 23.0 cm (9 in)[2] 79.5 cm (31 in)

Weight 18.75 kg (41 lb)

Power consump- 950 W - Maximum consumption

tion
300 W - Average during thermal cycling

10 W - During sleep/ standby

[1] At the rear of the instrument to ensure adequate airflow and cooling
[2] With the heated cover open

Insert image of Instrument

20 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Chapter 2 Instrument Setup
Environmental requirements 2

Environmental requirements
Temperature and Ensure that the installation site is maintained under the following conditions:
humidity
Table 5 Temperature and humidity requirements
requirements
Condition Acceptable range

Temperature 15 to 30°C (59 to 86°F)

Humidity 15 to 80% relative humidity, non-condens-

ing

Avoid placing the instrument adjacent to heaters, cooling ducts, or in direct sunlight.
Fluctuations between day and night temperatures can cause system instability. Place
away from any equipment that vibrates, such as a refrigerator or centrifuge.

Pollution The ProFlex™ PCR System has a Pollution Degree rating of 2. It may be installed in an
environment that has non-conductive pollutants only, such as dust particles or wood
chips. Typical environments with a Pollution Degree II rating are laboratories and
sales and commercial areas.

Altitude The safety of ProFlex™ PCR System use was tested for altitudes up to 2000 ft.

Materials

Required • Scissors, pocket knife, or box cutter

materials • Compressed air
• Protective hardware, as appropriate (see below)

Optional Life Technologies supports the use of the following devices to protect the ProFlex™
protective PCR System from damage resulting from electrical hazards and the resultant loss of
data. Before installing the system, decide what additional hardware (if any) you want
hardware
to install.
• Power line regulator (1.5-kVA)
• Surge protector/line conditioner (10-kVA)
• Uninterruptible power supply (1.5-kVA)

Unpacking the ProFlex™ System

IMPORTANT! Save the packing materials and box in case you need to ship the instru-
ment to Life Technologies for service.

1. To unpack the ProFlex™ System:

a. Cut the straps securing the instrument box.

ProFlex™ PCR System User Guide 21

For Research Use Only. Not for use in diagnostic procedures.
2 Chapter 2 Instrument Setup
Setting up the ProFlex™ System

b. Cut the tape securing the top flaps of the instrument crate, then open the
flaps.

c. Remove the ProFlex™ System Accessories from the instrument and set them
aside.

d. Lift and remove the cover from the instrument crate.

e. Remove the packing material from the ProFlex™ System, then inspect the in-
strument for shipping damage.

IMPORTANT! If the ProFlex™ System is damaged, note the location and ap-
pearance of the damage, then contact Life Technologies Technical Support
or your service representative (see “How to Obtain Support” on page XX -
insert link later).

2. Move the ProFlex™ System to the desired installation site. Follow these guide-
lines for lifting and moving:
• Make sure that you have a secure, comfortable grip.

• Keep your spine in a neutral position.

• Bend at the knees and lift with your legs.

• Do not lift and twist your torso at the same time.

3. Open the bag containing the ProFlex™ System Accessories, then verify that it con-
tains:
• Cable, NORTH AMERICA 15A/125V CORDSET (Part no. 4445007)

• Cable, EUROPE 16A/250V CORDSET (Part no. 4445005)

• ProFlex™ System Starter Kit

Setting up the ProFlex™ System

1. Remove the packing material:
a. Open the ProFlex™ System heated cover.

b. Remove the packing material from the ProFlex™ sample block(s) (ProFlex™
96-Well Sample Block or ProFlex™ 3x32-Well Sample Block).

c. Using a can of compressed air, deliver a blast of air into each well of the
sample block(s) to remove any particles that may have collected during
transportation.

2. Install the sample block. To place the sample block into the instrument base:
a. Facing the ProFlex™ Base unit away from you, pull the lever out from the
sample block .

22 ProFlex™ PCR System User Guide

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 Chapter 2 Instrument Setup
Setting up the ProFlex™ System 2

b. Place the sample block onto the ProFlex™ Base with the latching mechanism
to the right.

c. Push the latching mechanism all the way to the left to secure the block onto
the base.

Note: If the sample block module is not seated in place correctly, the instrument
will not be able to detect the block type.
Note: To change the sample block from one type to the other, see “Changing
sample blocks” on page 26.

3. Close the heated cover.

4. Connect the power cable to the ProFlex™ System.

5. (Optional) Install any of the recommended protective devices (see “Optional pro-
tective hardware” on page 21).

6. Connect the power cable to the 800 VA power source receptacle.

7. In the absence of the ethernet cable or the ethernet port, you can connect the in-
strument to the network via the USB-enabled Wi-Fi Card (Part no. 4483658) as
shown in the following graphic.
Insert the Wi-Fi Card into the USB port at the back of ProFlex™ System. For in-
structions on setting up the wireless connection, see “How to set up the wireless
connection in the ProFlex System” on page 24

8. Press the power button, then wait for the instrument to start up. Proceed with
the installation after the touchscreen displays the Main Menu, indicating that the
ProFlex™ System startup is complete.
When you power on the instrument, the instrument may require about 30 sec-
onds to start up.
The Home screen will display following successful installation.

ProFlex™ PCR System User Guide 23

For Research Use Only. Not for use in diagnostic procedures.
2 Chapter 2 Instrument Setup
How to set up the wireless connection in the ProFlex™ System

Figure 7 ProFlex™ 3x32-Well System Touchscreen Home

How to set up the wireless connection in the ProFlex™ System

See “Setting up the ProFlex System” on page 22 on connecting the USB-enabled Wi-Fi
Card into the ProFlex™ System. To set up the wireless connection:

1. Power on the ProFlex™ Instrument, and in the Home screen, touch Settings.

2. In the Settings screen, touch Device Settings.

3. In the Device Settings screen, touch Network Connection. In the Network Con-
nection Type screen, touch Wireless.
The Search Wireless Network screen will come up and the wireless symbol will
appear active. Touch Cancel to exit the screen.
Note: During initial setup, if you selected the Wired option in the Network Con-
nection Type screen, you will be required to enter your IP address if you selected
the Static IP wired option. If you selected the Dynamic IP wired option, the IP
address is automatically populated.

4. Once a wireless connection has been detected, a list of the available networks
will be displayed in the Choose Network screen. Touch the network name of
your choice or touch Join Other Network.

5. In the Find and Join a Network screen, enter the name and security type of the
network.
Note: When you touch in the Network Name field, a keypad will come up to
facilitate making the entry.

24 ProFlex™ PCR System User Guide

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 Chapter 2 Instrument Setup
How to set up the wireless connection in the ProFlex™ System 2

6. From the Security type drop-down menu, touch to select the security type and
enter the relevant information in the screen.
Note: Contact your IT Systems Administrator for information on security type.
You can select from the following options:
• WEP
• WPA Personal
• WPA2 Personal
• WPA Enterprise
• WPA2 Enterprise

7. Touch the Remember this network checkbox if you want the ProFlex™ System to
remember the network.
Touch Join to continue or Cancel to exit from the Find and Join a Network
screen.

8. Depending on the security type you have selected, enter the appropriate pass-
words and touch Join.
If you entered the correct information, the Network Connection Complete screen
will appear. Touch OK to continue. If you entered incorrect information, the Net-
work Connection Failed screen will come up and you will require to go back to
security type screen to resolve the issue. Touch OK to continue.

Figure 8 Setting up wireless connection in the ProFlex™ Instrument

ProFlex™ PCR System User Guide 25

For Research Use Only. Not for use in diagnostic procedures.
2 Chapter 2 Instrument Setup
Changing sample blocks

Changing sample blocks

The ProFlex™ System features an interchangeable sample block module allowing port-
ability and versatility in sample configuration.
The ProFlex™ System supports the following sample blocks:
• (ProFlex™ 96-Well Sample Block
• ProFlex™ 3x32-Well Sample Block
To change the sample block:

1. Pull lever out from the sample block module towards the right to loosen the
block from the base.

2. Remove the current sample block from the base.

3. Place the sample of block of your choice onto the base.

4. Pull the lever towards the left and into the instrument to secure the module.

IMPORTANT! Ensure that you don't change the block while the ProFlex™ Instru-
ment is powered on. Although the ProFlex™ System firmware supports the
change, the action can cause extreme damage to the hardware itself.

Connecting to a network printer

The ProFlex™ System requires a printer that:
• Has an Ethernet port
• Supports the LPD/LPR protocol (often called a “TCP/IP printer”)

1. Plug the Ethernet cable into the Ethernet port on the back of the instrument.

2. Plug the other end of the cable into a network port.

You must also configure the instrument firmware with printer information be-
fore you can print (see “Configuring the Instrument for a Printer” on page 2-8...
insert link later)... NOT SURE IF THIS FEATURE IS AVAILABLE FOR PHASE 1.

Connecting together multiple instruments

You will need Connecting your ProFlex™ System to other ProFlex™ Systems allows you to use one
instrument to control others. You can connect together more than 12 instruments at a
time.
To connect together multiple ProFlex™ Systems, you need:
• An Ethernet cable for each ProFlex™ System
• A network switch
• A TCP/IP printer, if you want to print

26 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Chapter 2 Instrument Setup
Setting up the ProFlex™ System touchscreen 2

For best results, do not connect the network switch to your laboratory’s network.
Communication between ProFlex™ Systems works best on an isolated system, free
from interference by other network traffic.

Making the 1. Connect the Ethernet cable to the back of the ProFlex™ System and connect the
connections other end of the cable to the network switch.

2. Repeat step 1 for each ProFlex™ System.

3. Connect the printer to the network switch.

4. Power on the network switch.

5. For each ProFlex™ System, follow the instructions in “Setting the IP Address” on
page 2-7 and “Setting the Instrument Name” on page 2-8... Insert links later.
• When you set the instrument’s IP address, select Assign IP address dynami‐
cally (using DHCP).

• Make sure each instrument name is unique.

6. Follow the instructions in “Configuring the Instrument for a Printer” on page

2-8... Insert link later.

7. Continue with “Powering On,” and “Setting Up the ProFlex™ System” on page
2-7... Insert links later.

Setting up the ProFlex™ System touchscreen

Recommended Before performing a run, be sure to set the following instrument settings:
instrument • Instrument IP address (page 2-7)
settings • Instrument name (page 2-8)
• Current date and time (page 2-8)
• Printer information, if you want to print run methods or the run report (page 2-8)
Optionally, you can also change the settings for:
• User accounts and security (page 2-9)
• Idle state temperatures for the cover (page 2-14)
• Durations for stand-by (page 2-15)
Insert links later

Setting the IP address (PLEASE CONFIRM STEPS 4 & 5)

The following instructions for setting the IP address are applicable irrespective of
your connection with the network via the ethernet cable or Wi-Fi.
Note: If your instrument is not on a network, you do not need to set the IP address.
The IP address can be assigned dynamically by a computer on the network (using
DHCP) or set manually by entering a number in the IP Address screen of the Pro-
Flex™ System Touchscreen.

ProFlex™ PCR System User Guide 27

For Research Use Only. Not for use in diagnostic procedures.
2 Chapter 2 Instrument Setup
Setting up the ProFlex™ System touchscreen

Note: Ask your system administrator if the IP address is assigned statically or dy-
namically. For static addresses, you need to know the IP address for the instrument,
the subnet mask, and the default gateway.

1. In the Home screen, touch Settings Menu.

2. In the Settings Menu screen, touch Device Settings.

3. In the Device Settings screen, touch Network Connection.

4. In the Network Connection screen, touch Wired:

• Dynamically:

28 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Chapter 2 Instrument Setup
Setting up the ProFlex™ System touchscreen 2

Touch Assign IP address dynamically (using DHCP). A check mark appears

when DHCP is selected.
or

• Manually: Touch Static, then enter the appropriate IP addresses for the in-
strument, the subnet mask, and, optionally, the default gateway using the nu-
meric editor. Addresses are in the form of X.X.X.X, where each X is a 3- digit
number, from 001 to 255.

5. Touch Done, then OK to save your changes and return to the Administrator Set-
tings screen.

Setting the instrument name (PLEASE CONFIRM STEPS 4 THROUGH 6)

By default the instrument name is set to the instrument serial number. You can
change the name to something more descriptive. The instrument name appears in the
About the Instrument and Run Report screens and, if you connect together multiple
instruments, in the Select Instruments screen.

1. In the Home screen, touch Settings Menu.

2. In the Settings Menu screen, touch Device Settings.

3. In the Administrator Settings screen, touch Instrument Name.

4. Touch the field to enter the new name.

Note: Ensure that the names do not contain spaces and do not have more than
24 alphanumeric characters or, for numeric-only names, do not have more than
10 characters.

5. Touch Yes next to Allow Remote Runs to make the instrument available for re-
mote runs (a run on this instrument can be started from another instrument); oth-
erwise touch No.

ProFlex™ PCR System User Guide 29

For Research Use Only. Not for use in diagnostic procedures.
2 Chapter 2 Instrument Setup
Setting up the ProFlex™ System touchscreen

6. Touch Done, then OK to save your changes and return to the Administrator Set-
tings screen.

Note: If you edit the Instrument Name field, you can still view the serial number in
the About the Instrument screen. You can access the About the Instrument screen
from either the Login screen or the Settings Menu screen.

Configuring the instrument for a printer

If you want to print the run report, test results, and/or a run method, you must config-
ure the instrument with information about the printer.

1. In the Home screen, touch Settings Menu.

2. In the Settings Menu screen, touch Device Settings.

3. Touch the fields to set the printer’s name and IP address.

4. Touch Done to save your changes and return to the Settings Menu screen.

Setting the date and time (PLEASE CONFIRM STEPS 4 THROUGH 6)

The date and time appear in the run report and at the bottom of many of the screens
on the instrument.

1. In the Home screen, touch Settings Menu.

2. In the Settings Menu screen, touch Device Settings.

3. In the Device Settings screen, touch Date / Time.

4. To set the date, touch Date, then enter today’s date, using the format YYYYMM-
DD. You also need to enter the “/ " character.

5. To set the time, touch Time, enter the current time using the format HH:MM,
then touch AM or PM. You also need to enter the “:” character.

6. Touch Done, then OK to save your changes and return to the Administrator Set-
tings screen.

Adding users Access levels (NEED CONTENT FOR SECURITY LEVELS)

(screenshots to be Each user is assigned an account type that determines how you can interact with the
updated) instrument. There are three account types:
• Limited User – Can perform runs, view and print run methods, and run tests.
Cannot edit or create run methods, manage users, or set instrument preferences.
• User – Can perform runs, create, view, edit, delete, save and print run methods,
and run tests. Cannot manage users or set instrument preferences.
• Administrator – Can access all instrument functions, including setting security,
managing users and settings instrument preferences.
Each user is also assigned to a different level of security. There are three security lev-
els:
• Open mode –

30 ProFlex™ PCR System User Guide

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 Chapter 2 Instrument Setup
Setting up the ProFlex™ System touchscreen 2

• Restricted 1 –
• Restricted 2 –

Adding yourself as a user (NEED NEW CONTENT)

If you set the instrument to require login, you need to use the default administrator
user name and password until you create your own user account.
To add yourself as a user:

1. In the opening screen, touch On, then Log In. (insert screenshot)

2. In the Log In screen, touch:

a. The User field, then enter admin.

b. The Password field, then enter password.

c. Log In.

3. In the Home screen, touch Settings Menu.

4. In the Settings Menu screen, touch Administrator Settings.

5. Follow the instructions in 'Adding users' (add link) to add yourself as a user.
Make sure you make yourself an Administrator.

Adding users
To add a user:

1. Log in as a user with Administrator access.

2. In the Home screen, touch Settings Menu.

3. In the Settings Menu screen, touch Administrator Settings.

4. In the Administrator Settings screen, touch Manage Users.

The Manage Users screen is displayed: (insert ss)

5. Touch Add User.

The Create New User Account screen is displayed: (insert ss)

6. Enter information for the new user. The required fields are User Name, Pass-
word, Re-enter Password, and Permission Level.
a. User Name must be between 5 and 15 characters, with no spaces.

b. Password must be at least 1 character.

c. Touch Set Access, then touch one of the following:

• Limited User
• User
• Administrator

7. Touch Done, then OK to add the user account to the instrument.

ProFlex™ PCR System User Guide 31

For Research Use Only. Not for use in diagnostic procedures.
2 Chapter 2 Instrument Setup
Setting other options

The new user appears in the Manage Accounts screen.

8. When you finish, touch (insert graphic of 'Close') to return to the Administrator
Settings screen.

Changing users (NEED CONTENT)

To edit a user:

Deleting users (NEED TO UPDATE CONTENT HERE)

To delete a user:

1. Log in as a user with Administrator access.

2. In the Main Menu screen, touch Settings Menu.

3. In the Settings Menu screen, touch Administrator Settings.

4. In the Administrator Settings screen, touch Manage Users.

The Manage Accounts screen is displayed: (insert screenshot)

5. In the list, touch the user name for the account you want to delete, then touch
Delete User.
A confirmation message appears.

6. Touch OK to delete the user and any run methods or folders for that user, then
OK.

7. When you finish, touch (Insert symbol graphic Close) to return to the Adminis-
trator Settings.

Setting other options

Table 6 lists additional options that can be adjusted or activated. These settings apply
to all users.

Table 6 Additional options (and their default values) for the ProFlex™ System

Option Default value See page

Idle temperatures for the cover and Cover: 105°C insert link lat-
the sample block(s) er

Durations for standby time-outs Cover: 10 sec insert link lat-

er
Standby: 1 min. 30 sec.

Setting idle 1. In the Main Menu screen, touch Settings Menu.

temperatures for
2. In the Settings Menu screen, touch Set Idle Temperatures (this is not part of the
the heated cover
ProFlex Settings screen).
(PLEASE

32 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Chapter 2 Instrument Setup
Setting other options 2

CONFIRM STEPS 2 3. In the Idle State Temperatures screen, to set the temperatures for the cover,
THROUGH 4) touch:
a. Enabled to turn the option on. If this is disabled, the temperature for the
cover is not maintained by the instrument and will eventually come to room
temperature.

b. The Temperature field, then enter the temperature. The temperature for the
cover must be between 30 and 110°C.

4. Touch Done to save your changes and return to the Settings Menu screen.

Setting time-outs 1. In the Home screen, touch Settings Menu.

(NEED NEW
2. In the Settings Menu screen, touch Set Time‐outs. (Insert screenshot)
CONTENT)
3. In the Set Time-Outs screen, touch Time‐out, then enter the duration.
• Default Standby Time-Out sets the duration that the instrument is idle before
the touchscreen is turned off. Enter a duration of 00:00 if you do not want the
instrument to turn off the touchscreen.

• Default Pause Duration sets the duration the instrument pauses, after you
touch Pause during a run.

4. Touch Done, then OK to save your changes and return to the Settings Menu
screen.

ProFlex™ PCR System User Guide 33

For Research Use Only. Not for use in diagnostic procedures.
 3 Running PCR Samples

Loading samples into the instrument

See the appropriate section for your instrument:
• Loading samples in ProFlex™ 96-Well PCR System (insert link later)
• Loading samples in ProFlex™ 3x32-Well PCR System (insert link later)

Loading samples This section describes how to load samples in:

into the ProFlex™ • Tubes with attached caps
96-Well PCR • 96-well reaction plates
System • 96-well tray/retainer assemblies

Loading tubes with attached caps

There is no need to use mineral oil or glycerine in the sample wells or as a vapor bar-
rier over the PCR reaction mixture in the tubes. The MicroAmp® Reaction Tubes fit
tightly in the wells, and the heated cover exerts an even pressure on all tubes, elimi-
nating condensation on the tubes.

1. Set the 96-well tray on a splash-free 96-well base.

2. Place the reaction tubes in the tray.

3. Pipette the samples into the reaction tubes.

4. Cap the tubes.

5. See Placing the Sample Tray or Plate onto the Sample Block(s) (Insert link later).

Loading the reaction plate

1. Place the reaction plate on a splash-free 96-well base.

2. Pipette the samples into the sample wells.

3. Cap the plates using the MicroAmp™ 8-Cap Strips, or cover the plates with Mi-
croAmp™ Clear Adhesive Film.

4. See Placing the Sample Tray or Plate onto the Sample Block(s) (Insert link later) .

34 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Chapter 3 Running PCR Samples
Loading samples into the instrument 3

Loading tubes without caps

To load the 96-well tray/retainer:

1. Place the tray on the splash-free 96-well base.

2. Load tubes onto the tray using either single tubes or the 8-tube strips.

3. Place the retainer over the tubes.

4. Pipette the sample into the tubes.

5. Cap the tubes using a MicroAmp™ 8-Cap Strip.

6. See Placing the Sample Tray or Plate onto the Sample Block(s) on page 3-6 (insert
link later).

Placing the sample tray or plate onto the sample block(s)

The steps for placing the sample tray in the sample block(s) are the same for:
• A sample tray/retainer
• A sample tray without a retainer
• A 96-well plate.

1. Lift the sample tray from the splash-free 96-well base and place it in the sample
block(s).

2. Place the MicroAmp™ Tray or Plate onto the sample block(s) so that well A1 is at
the upper left corner of the tray, as shown in the following figure:

3. Close the heated cover.

Loading samples This section describes how to load samples into the MicroAmp® reaction tubes or the
in the ProFlex™ MicroAmp® tube strips.
3x32-Well PCR 1. Pipette the samples into the MicroAmp® reaction tubes or the MicroAmp® tube
System strips.

2. If you are using the MicroAmp® tube strips, secure the tube strip with the Micro-
Amp® cap strip. You can use the MicroAmp® Cap Installing Tool (Handle)

3. Place the MicroAmp® reaction tubes or the MicroAmp® tube strips into the re-
tainer.

4. Place the retainer onto the sample block.

ProFlex™ PCR System User Guide 35

For Research Use Only. Not for use in diagnostic procedures.
3 Chapter 3 Running PCR Samples
Creating a new run method (PLEASE CONFIRM CONTENT. SCREENSHOTS TO
BE UPDATED IN NEXT DRAFT)

5. Close the heated cover.

Creating a new run method (PLEASE CONFIRM CONTENT.

SCREENSHOTS TO BE UPDATED IN NEXT DRAFT)
1. In the Home screen, touch New Method.

2. Select a template.

3. In the edit mode, edit the parameters of the method template such as tempera-
ture, time, reaction volume, number of steps/stages.
• Insert a stage (see “Adding a Stage” on page 3-10).

• Insert a step (see “Adding a Step” on page 3-11).

• Add an AutoDelta step (see “Creating an AutoDelta Step” on page 3-13).

• Add a pause (see “Adding a Pause” on page 3-15).

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 Chapter 3 Running PCR Samples
Performing a run using an existing method 3

• Delete a step or stage (see “Deleting a Stage or Step” on page 3-15).

• Add a step to program different temperatures on the VeriFlex™ Blocks.

(see “Creating a VeriFlex™ Step” on page 3-16).

4. Touch Save & Exit to display the Save Run Method screen. (insert screenshot).
Note: You can also start a run without saving the run method.
a. Touch Run Method to enter the name of the run method. Names can be up
to 100 characters. Names may not contain any of the following characters:
%*?|;:,!@#$.()<>/\"'`~{}[]=+&^ (tab). Spaces, underscores, and dashes are al-
lowed.

b. Touch (insert graphic)(Folder) to select a folder in which to save the run

method.
Note: To save the run method to the USB drive, touch USB (available only
when a USB drive is inserted in the instrument).

5. Touch Save & Exit when you are done.

Performing a run using an existing method

To perform a run using an existing run method:

1. In the Home screen, touch Open Method to open the Select Method screen.

2. In the Select Method screen, touch a run method under the Method name to edit
that run method in the edit mode.

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3 Chapter 3 Running PCR Samples
Performing a run using an existing method

Figure 9 Select Method screen

The following screen serves as a preview of the run method. If you do not want
to edit any of the parameters, you can start a run directly by touching Choose
Block (button to change to Start Run in subsequent drafts).

Figure 10 Preview of Run Method

3. In the run method screen that you have selected, touch Edit or touch any of the
values to start editing the parameters.
There are various parameters for editing a run method, from basic editing of
temperatures, time, adding/deleting a stage/step, to advanced options of VeriFlex
for optimization, AutoDelta, ramp rates, and simulation modes.

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 Chapter 3 Running PCR Samples
Performing a run using an existing method 3

Different options appear as you edit.

4. Touch Manage Steps to manage the steps; for example, add or delete a stage or
step, or set more advanced parameters.

Figure 11 Options available in the Run Method preview screen

a. Touch Add Steps to add a step.

b. Touch Remove Steps to delete a step.

c. Touch Add Stages to add a stage.

d. Touch Remove Stages to delete a stage.

The '+' buttons appear while adding a step or stage, while the '–' buttons appear
while deleting a step or stage.

ProFlex™ PCR System User Guide 39

For Research Use Only. Not for use in diagnostic procedures.
3 Chapter 3 Running PCR Samples
Performing a run using an existing method

Figure 12 Manage Steps screen

You can finish editing in this screen and start a run without saving the method.

5. Touch Advanced Options to edit the VeriFlex, Auto-Delta, Ramp Rates, and
Simulation Mode parameters.

Figure 13 Advanced Options screen

• Touch Simulation Mode to edit the simulation mode (add screen details and
screenshot later).

• Touch Ramp Rates to edit the ramp rate of the individual steps. Touch Save
As to save the changes, then touch Done.

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 Chapter 3 Running PCR Samples
Performing a run using an existing method 3

Figure 14 Advanced Options — Ramp Rates

• Touch Veri‐Flex to edit the Veri-Flex parameter.

a. Choose the steps you want to apply Veri-Flex.

Figure 15 Advanced Options — Veri-Flex

b. In the Edit Veri-Flex screen, adjust the temperature range across the
block for individual steps. Use the and to scroll between the
stages.

ProFlex™ PCR System User Guide 41

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3 Chapter 3 Running PCR Samples
Performing a run using an existing method

Figure 16 Edit Veri-Flex

– Touch Remove Veri‐Flex to remove the Veri-Flex step.

– Touch Done when finished or Cancel to exit the screen.
c. Touch Save As to save the changes, then touch Done.

• Touch Auto‐Delta to apply Auto-Delta to a step.

a. Choose the steps you want to apply Auto-Delta.

Figure 17 Advanced Options — Auto-Delta

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 Chapter 3 Running PCR Samples
Performing a run using an existing method 3

b. In the Edit Auto-Delta screen, enter the information for setting up Auto-
Delta.
– Enter the Starting Cycle.
– Use the and to increase and decrease, respectively, the DEL-
TA temperature and time.
– Touch Done when finished or Cancel if you want to exit the screen.
– Touch Remove Auto‐Delta to remove the Auto-Delta.

Figure 18 Edit Auto-Delta

c. Touch Save As to save the changes, then touch Done.

Once you have edited a run method, you can save the method to the destination
folder by touching Save As.

6. Start a run with the selected run method that is opened and edited by selecting
the idle block(s) and touching Start Run. The Run ID serves as a unique identifi-
er for the particular run. Touch Add Comment to add comments for the run.

ProFlex™ PCR System User Guide 43

For Research Use Only. Not for use in diagnostic procedures.
3 Chapter 3 Running PCR Samples
Performing a run using an existing method

Figure 19 Start a run

Alternatively, you can start a run by selecting an idle block in the Home screen
and touch Set Up Run. (tweaked screenshot, needs to be replaced)

Touching Set Up Run will take you to the Set Up Run screen that displays the
following:
• Open Templates: Touch Open Template to create a new run method with
the help of the default templates and start a run.
• Open Methods: Touch Open Method to open an existing run method, edit
the parameters, and start a run.
• Incubate: Touch Incubate to use the ProFlex™ Instrument as a refrigerator or
heater.

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 Chapter 3 Running PCR Samples
Editing details (content and screenshots to be updated) 3

Note: For detailed instructions on starting a run, see “Performing a run (please
confirm content)” on page 48

Editing details (content and screenshots to be updated)

This section describes how to edit a run method by: (insert links later)
• Adding a stage (page 3-10)
• Adding a step (page 3-11)
• Editing a temperature or a time (page 3-12)
• Editing a ramp rate (page 3-12)
• Creating an AutoDelta step (page 3-13)
• Adding a pause (page 3-15)
• Creating a step to program different temperatures on the VeriFlex™Blocks (page
3-16) (Available only on the ProFlex™ 96-Well PCR Systems.)

Adding a stage The two types of run method stages are:

• Cycling – The steps in a cycling stage are repeated a specified number of times.
In general, the steps in a cycling stage are template denaturation, primer anneal-
ing, and primer extension.
• Holding – The steps in a holding stage are not repeated.
To add a stage:

1. In the Edit Run Method screen, touch the stage name (at the top of the stage) to
select it. When a stage is selected, the area around the name is red. (Insert screen-
shot)

2. Touch Add.
A copy of the selected stage is added to the run method.

3. For a cycling stage, edit the number of cycles as needed.

a. Touch the stage to select it.

b. Touch the cycles field (the box below the Stage Name) to select it (insert
screenshot):

c. Enter the number of cycles, then touch Done.

4. To change the temperature and/or time for any step in the stage, see “Editing
Temperature and Time” on page 3-12 (insert link later).

Adding a step 1. In the Edit Run Method screen, touch anywhere in the column for a step to select
it.
When the step is selected, the column turns red: (insert screenshot)

2. Touch Add.
A copy of the selected step is added to the run method: (Add screenshot)

ProFlex™ PCR System User Guide 45

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3 Chapter 3 Running PCR Samples
Editing details (content and screenshots to be updated)

3. To change the temperature and/or time for the step, see “Editing Temperature
and Time” on page 3-12 (insert link later).

Editing 1. Touch any temperature field to select it, then edit the temperature as desired.
temperature and or
time Touch any time field to select it, then edit the time as desired.

2. You can edit any other times, temperatures, (or the number of cycles for a stage)
without closing the editor.
a. Touch the field.

b. Touch the keys in the editor.

c. Repeat for any other field.

3. Touch Done to close the editor.

Editing ramp rates You can edit the rate at which the instrument ramps to the temperature for the step.
The default ramp rate is 100%.
Note: You cannot edit ramp rates for methods created using the Convert Method
wizard (see “Using GeneAmp® PCR System 9700 Run Methods” on page 5-2 - insert
link later).

1. Touch a step to select it.

2. Touch Options, then Ramp Rate.

3. Touch the ramp field, then enter the desired ramp rate. Ramp rates from 0.1 to
100% are valid.

4. Touch Done to close the editor.

The ramp rate symbol (insert symbol graphic) appears next to any step with a
ramp rate less than 100%.

Creating an An AutoDelta step allows you to increase or decrease the temperature and/or change
AutoDelta step the duration of the cycle during a cycling stage.
One use for an AutoDelta step is for touchdown PCR, a method in which the earliest
cycles have a high annealing temperature, followed by cycles where the annealing
temperature decreases slightly for each cycle. Touchdown PCR increases the specifici-
ty of the PCR products. The high initial temperature favors more specific annealing
between the primer and the template. Because these fragments are further amplified
during subsequent cycles at lower temperatures, they outcompete the nonspecific se-
quences to which the primers bind at lower temperatures.
Note: AutoDelta steps are allowed only in cycling stages.
To create an AutoDelta step:

1. Touch a step, then Options.

The options menu appears: (insert screenshot)

2. In the Options menu, touch AutoDelta.

The Create an AutoDelta Step screen is displayed: (insert screenshot)

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 Chapter 3 Running PCR Samples
Editing details (content and screenshots to be updated) 3

3. Touch Starting Cycle, then enter the cycle at which the AutoDelta step begins.

4. Select the parameter(s) to increment:

• Touch Delta Temperature, then enter the number of degrees that the temper-
ature changes for each cycle. To enter a temperature that decreases with every
cycle, touch + to change it to −. To enter a temperature that increases, the but-
ton should show +.
and/or

• Touch Delta Time, then enter the minutes and seconds that the step duration
should change with each cycle. To enter a time that decreases with each cycle,
touch + to change it to −. To enter a time that increases, the button should
show +.

Note: Make sure the parameters you enter result in a final temperature greater
than 4.0°C and less than 99.9°C. Also, the Delta Time must be less than the total
time for the stage.

5. Touch OK to save the step and return to the screen that displays the run method.
The AutoDelta symbol (insert graphic symbol) appears on the step with the Au-
toDelta step. In this example, Step 2 in Stage 2 is an AutoDelta step: (insert
screenshot)

Deleting a stage 1. In the Edit Run Method screen, touch the stage or step to select it.
or step
2. Touch Delete, then OK.
The stage or step is deleted from the run method.

Creating a Note: This function is only available for the 96-well versions of the ProFlex™ 96-Well
VeriFlex™ step PCR System.

The ProFlex™ 96-Well PCR Systems has one VeriFlex™ block. You can specify temper-
atures for the VeriFlex™ block. The VeriFlex™ block can be used to provide up to six
different temperature zones for the 96 wells. A step that has different temperatures is
called a “VeriFlex™ step.” This feature is useful for quickly optimizing new run meth-
ods.
To create a step with different temperatures for the VeriFlex™ block:

1. Touch a step, then Options.

The options menu appears: (insert screenshot)

2. In the Options menu, touch VeriFlex step.

The Create a VeriFlex Step screen is displayed: (insert screenshot)

3. Touch a temperature field, then enter the desired temperature. Temperatures for
neighboring VeriFlex™ blocks can be the same, but if they are not the same, they
must be at least 0.1°C apart but no more than 5.0°C apart. The maximum temper-
ature is 99.9°C.

4. Depending on your instrument configuration, enter either a duration or a ramp

rate.
• Touch Time, enter the duration (in minutes and seconds), then touch Done.

ProFlex™ PCR System User Guide 47

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3 Chapter 3 Running PCR Samples
Performing a run (please confirm content)

or

• Touch Ramp Rate, enter the ramp rate (as a percentage value), then touch
Done.

The VeriFlex Step appears in the Edit Run Method screen, with the text “Zones”
in the temperature field.

Performing a run (please confirm content)

Prepare your samples and load the sample block(s) as described in “Loading samples
into the instrument” on page 34.

IMPORTANT! You can start a run on multiple instruments at the same time if your
instruments are on a network.

To start a run from the Home screen:

1. Close the cover.

2. Touch Open Method or New Method.

Note: See “Creating a new run method (PLEASE CONFIRM CONTENT.
SCREENSHOTS TO BE UPDATED IN NEXT DRAFT)” on page 36 to create a
new run method or see “Performing a run using an existing method” on page 37
to use an existing run method.

3. Locate the run method you want to use, then touch the run method to select it.

4. Touch Start Run.

The Run Parameters screen is displayed:

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 Chapter 3 Running PCR Samples
Monitoring a run (please confirm content) 3

Verify the name of the run method that you have selected, and in case of the Pro-
Flex™ 3x32 Well PCR System touch the dial corresponding to the block that you
are using to perform the run.
Reaction volumes are specific to the type of instrument:
• ProFlex™ 1x96 Well PCR System: 10 to 100 µL
• ProFlex™ 3x32 Well PCR System: 10 to 100 µL

5. Touch Run ID, then enter the applicable information.

The default Run ID is YYYYMMDDHHMM, where “YYYYMMDD” is the date
and “HHMM” is the current time.

6. If you are making runs on: (PLEASE CONFIRM STEPS 6 AND 7)

• Only this instrument – Touch Start Run Now, then OK. The cover is heated
to the required temperature, then the run begins. When the run is complete,
the Run Report is displayed.
or

• Multiple instruments – Touch Start Multiple Runs. The Select Instrument

screen is displayed: (insert screenshot)

7. In the Select Instrument screen:

a. Touch the check box corresponding to each instrument on which you want
to perform the run. Only instruments that are compatible with the run
method are available. Incompatible instruments are indicated by (insert
graphic symbol). If you want to perform the run on this instrument, be sure
to touch the check box in the upper left corner of the screen.

b. Touch Start Run Now, then OK. The run method is sent to each selected in-
strument, and, if no problems are encountered, the run begins. After the run
begins, you must interact with any remote instruments directly if you want
to make changes to the run method, pause, or stop the run. When the runs
are complete, the Run Reports are displayed on each instrument.

8. While the run is in progress, the Home screen is displayed:

Note: There are two ways of monitoring a run from the ProFlex™ touchscreen:
· From the Home screen
· From the Run Monitor screen
For more information on monitoring a run, see “Monitoring a run (please con-
firm content)” on page 49.
Note: If you want to stop a run midway, touch in the run monitor
screen.

Monitoring a run (please confirm content)

Monitoring via the After a run has started, the Home screen appears again with the dials updated with
Home screen current information on the run. The dials display the following run information:
• Current block temperature

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 3 Chapter 3 Running PCR Samples
Monitoring a run (please confirm content)

• Time elapsed since the run started

• Run status
To monitor the run progress, touch the dial that is running to go the Run Monitoring
screen.
(screenshot to be added)

Monitoring via the You can follow the status of a run using the Run Monitor screen as shown in the fol-
run monitor lowing figure:
screen

The screen shows the run method profile and additional information that does not ap-
pear in the Dial on the Home screen.

Table 7 Elements of the Run Monitor screen

Element Description

Start Time Displays the time a run started.

Time Remaining Displays the time remaining for a run.

~ End Time Displays the approximate time at which the

run will end.

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 Chapter 3 Running PCR Samples
Monitoring a run (please confirm content) 3

Element Description

Provides more details about the run.

Touching Details brings up a pop-up with
the following run information:
• Start time
• End time
• User
• Errors
• Comments
• Block temperature
• Cover temperature

Helps change the cycles of the stages that

are still in progress or have not yet started.
(NEED INFORMATION ON SKIP FEATURE)

Helps pause a run. Touching Pause brings

up a screen with the details of the paused
run.

Helps stop a run. After a run has completed

or been aborted, the Home screen appears
again.

Actions in the Run Monitor screen during a run (PLEASE CONFIRM CONTENT)
During a run, the touchscreen is active, allowing you to perform the actions indicated
in the following table (table to include graphics of the respective symbols/ Action but-
tons).

Table 8 Actions in the Run Monitor screen during a run

To... Touch...

View other stages and buttons.

Pause the run Pause (see “Pausing a Run” on page 3-23 insert link
later).

View run method information View Method Information.

The information you entered in the Run Parameters di-
alog box is shown

Display the time for the run Display Method Time.

The elapsed time and time remaining for the run are
shown.

Skip to the next step SKIP.

Skip works only when the instrument is holding.

Stop the run STOP (see “Stopping a Run” on page 3-23 - insert link
later).

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3 Chapter 3 Running PCR Samples
Monitoring a run (please confirm content)

To... Touch...

Change the number of cycles for The Cycle field then change the number of cycles (see
a cycling stage “Changing the Number of Cycles” on page 3-22 - in-
sert link later).

Go to the Home screen (graphic) Home. From the Home screen, you can ac-
cess most of the ProFlex™ System functions while the
run continues.

View the Status Report (graphic) Status.

Changing the number of cycles (PLEASE CONFIRM CONTENT)

You can change the number of cycles for a cycling stage while a run is in progress.

1. If needed, click the orange run monitor button to return to the run monitor
screen.

2. Touch the cycles field (below the Stage Name) for a cycling stage, then enter the
new value. You cannot:
• Edit a stage that is already complete.

• Enter a number that is smaller than the number of cycles that have already
been completed.

3. Touch Done to return to the Run Monitor screen.

The run continues, using the new number of cycles.

Pausing a run (SECTION TO BE UPDATED)

1. In the Run Monitor screen, touch Pause.
The Pause dialog box is displayed: (insert screenshot)
The run pauses for the duration set in the Set Time-Outs screen (see page 2-15 -
insert link later). (PLEASE CONFIRM STEP)
In the Pause Run dialog box:
• Remaining Time shows the remaining time for the pause.
• Elapsed Time is continually updated to show the time the run has been in
process.

2. If you want to:

• Pause until the default pause has elapsed – Do nothing.

• Pause for a specific amount of time – Touch Edit Time, enter the duration of
the pause, then touch Done. The run resumes when the pause is complete.

• Resume the run immediately – Touch Resume Run (to the left of the dialog
box).

Stopping a run
In the Run Monitor screen, touch Stop, then OK.

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 Chapter 3 Running PCR Samples
Viewing and printing the run report (PLEASE CONFIRM CONTENT) (NEED 3
SCREENSHOTS)

The run stops and the Home screen with the dials is displayed.

If the power fails If a power failure occurs during a run, the instrument attempts to continue the run
when the power returns. The run begins at the first step of the stage where the power
failed.
For example, if the power fails during step 2 of the tenth cycle for a stage with three
steps and 20 cycles, then after the power returns, the run starts at step 1 of the tenth
cycle and continues for 11 more cycles.
Note: If the time between the initial power failure and the time the power returns is
more than 12 hours, the run is not continued.

Viewing and printing the run report (PLEASE CONFIRM CONTENT)

(NEED SCREENSHOTS)
At the end of a run, the Run Report screen is displayed (below). If the last stage of the
run method is an infinite hold, you must touch Stop, then OK in the message box be-
fore the Run Report is displayed.
You can also see the Run Report by touching View Last Run in the Tools Menu: (in-
sert screenshot)
Note: The run report is saved only until the next run is finished.
· To print the run report, in the Run Report screen, touch Print.
If any errors occurred during the run, the error information is also printed.
· To save the run report to the USB drive, touch Save.

Removing the samples from the instrument

Grasp the sample tray/retainer assembly or reaction plate firmly with both hands and
gently rock it back and forth until the tubes (or plate) are free.

CAUTION! Sample caps may pop off if the cover is opened when the sample
block(s) temperature is above 27 °C.

ProFlex™ PCR System User Guide 53

For Research Use Only. Not for use in diagnostic procedures.
 ProFlex™ 3x32 Well PCR System

Do we need a separate chapter to describe this block?

54 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 ProFlex™ 1x96 Well PCR System

Do we need a separate chapter to cover information on this block?

ProFlex™ PCR System User Guide 55

For Research Use Only. Not for use in diagnostic procedures.
 4 Routine Maintenance

Scheduled maintenance (SECTION TO BE DELETED?? )

Run the following tests annually:
• Cycle Performance Test
• Heated Cover Verification
• Temperature Verification

As-needed maintenance
Tests Run the Temperature Nonuniformity Test (see page 5-13).

Cleaning the
instrument (NEED Preparation
SOME Before cleaning the instrument:
CONCEPTUAL
CONTENT HERE) 1. Power off the ProFlex™ Instrument by disconnecting the power.

2. Allow the instrument to cool until the heated cover and sample block(s) reach
room temperature.

Cleaning the touchscreen

Clean the touchscreen with any commercially available LCD cleaning product. Be
careful not to scratch the screen.

56 ProFlex™ PCR System User Guide

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 Chapter 4 Routine Maintenance
As-needed maintenance 4

Cleaning the sample wells

If you use any cleaning or decontamination method, except those recommended in
the manual, you risk damaging the equipment. Clean the sample wells once a month
or as needed.

WARNING! Always wear protective glasses and gloves when servicing the in-
strument. Also, make sure you disconnect the instrument from AC line power
before you begin any service procedure. Always wear protective glasses and
gloves when servicing the instrument. Also, make sure you disconnect the in-
strument from AC line power before you begin any service procedure.

CAUTION! During instrument operation, the temperature of the heated cover

can be as high as 113°C, and the temperature of the sample block(s) can be as
high as 100°C. Before performing the procedure, keep hands away until the
heated cover and sample block(s) reach room temperature.

To clean the sample wells:

1. Follow the steps in “Preparation” on page 56.

2. Open the heated cover.

3. Remove the sample tray from the sample block(s) and set it aside.

4. Use a cotton swab soaked in isopropanol to clean the sample wells thoroughly.
Make certain that the isopropanol has evaporated completely before reloading a
sample tray.

Decontaminating the sample wells

If the sample wells become contaminated with any biological agents, clean the wells
thoroughly with a cotton swab soaked in 1:10 v/v dilution of 5.25% sodium hypo-
chlorite (Chlorox bleach).
Use bleach solution in moderation. Applied Biosystems recommends the use of 10%
bleach solution for removing contamination from the Veriti™ instrument sample
block(s); excessive use of the solution, however, can corrode the sample block(s) mate-
rial. To prevent damage to the sample block(s):
• Avoid applying excessive amounts of bleach solution. If possible, instead of using
a squeeze bottle or soaked cloth, use an atomizer to deliver the solution to the
wells of the block(s).
• After treating with bleach solution, rinse the sample block(s) thoroughly using
deionized water.
Note: Removing residual bleach from the surfaces of the Veriti™ instrument using
water minimizes the long-term effects of bleach treatments.
If the sample block(s) become contaminated with radioactivity, use a commercially
available decontaminant to remove the contamination. If the block(s) cannot be de-
contaminated, the instrument cannot be returned to Applied Biosystems for service.

ProFlex™ PCR System User Guide 57

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4 Chapter 4 Routine Maintenance
As-needed maintenance

Cleaning the heated cover

Clean the heated cover once a month or as needed.

CAUTION! During instrument operation, the temperature of the heated cover

can be as high as 113°C, and the temperature of the sample block(s) can be as
high as 100°C. Before performing the procedure, keep hands away until the
heated cover and sample block(s) reach room temperature.

To clean the heated cover:

1. Follow the steps in “Preparation” on page 56.

2. Open the heated cover.

3. Soak a cotton swab or piece of clean cloth with isopropanol and gently wipe the
heated platen.

WARNING! CHEMICAL HAZARD. Isopropanol is a flammable liquid

and vapor. Exposure may cause eye, skin, and upper respiratory tract irri-
tation. Prolonged or repeated contact may dry skin and cause irritation.
Exposure may cause central nervous system effects such as drowsiness,
dizziness, and headache. Read the MSDS, and follow the handling instruc-
tions.

4. Remove any remaining isopropanol from the cover.

Note: If the platen becomes contaminated with amplified DNA, then raise the
heated cover to the cleaning position, wipe the platen with a cloth or cotton swab
soaked in bleach, then rinse with water.
Clean the heated platen once a month or as needed.

Decontaminate the heated cover

If the heated cover become contaminated with amplified DNA, raise the heated cover
to the cleaning position and wipe the cover with a cloth or cotton swab soaked in 1:10
v/v dilution of 5.25% sodium hypochlorite (Chlorox bleach), then wipe the cover with
a damp cloth.

Replacing fuses
Replacing fuses (DO WE NEED ANY CONCEPTUAL CONTENT HERE?)

Required materials
• Two 10 A Type M 250 V 5 × 20 mm fuses
• Fine flat-tip screwdriver

58 ProFlex™ PCR System User Guide

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 Chapter 4 Routine Maintenance
Ordering kits and replacement parts 4

Procedure
1. Power off the ProFlex™ instrument by disconnecting the power.

DANGER! ELECTRICAL SHOCK HAZARD. Severe electrical shock,

which could cause physical injury or death, can result from working on an
instrument when the high voltage power supply is operating. To avoid
electrical shock, disconnect the power supply to the instrument, unplug
the power cord, and wait at least 1 minute before working on the instru-
ment.

The fuses are in the fuse holder (F1, F2).

2. Insert a small flat-tip screwdriver into the slot in the upper portion of the power
entry module, and open the door.

3. Pull out the fuse compartment to inspect the two fuses in the fuse compartment:

4. Pull out the blown fuse(s) from the fuse compartment and replace with new
12.5A Type Time Lag 250V.

5. Place the fuse compartment back into the power entry module (there is only one
orientation) and close the door.

6. Press the door until it locks in place.

7. Connect the instrument power cord.

Ordering kits and replacement parts

Refer to Appendix A “Consumables” on page 63 to obtain part numbers for kits and
replacement parts.

ProFlex™ PCR System User Guide 59

For Research Use Only. Not for use in diagnostic procedures.
 5 Utilities

Simulation modes (screen are WIP. Section to be updated in next

draft)
Content to come from Advanced Options screens in the eGUI.

Upgrading system firmware

About the upgrade If Life Technologies provides a new version of the firmware for the ProFlex™ PCR Sys-
tem, you may be required to load the new firmware on the instrument.
You will need a USB drive and, if your instrument requires login, Administrator ac-
cess to upgrade the firmware.

IMPORTANT! You cannot upgrade the firmware while a run is in progress.

Upgrading the 1. Open the About Instrument screen to determine the version number of the exist-
ProFlex™ PCR ing firmware. To open the About Instrument screen, in the Home screen, touch
Settings. In the Settings screen, touch About Instrument.
System firmware
(PLEASE 2. Record the version number of the firmware, then touch Done.
CONFIRM STEP 3)
3. On a computer connected to the Internet, go to www.lifetechnologies.com to de-
termine the version number of the new firmware.

4. Click Support, then Software Downloads.

5. Select ProFlex™ PCR System in the list, then click Updates & Patches.

6. Find the appropriate file. If the version number is:

• The same as the current version (from step 3), you do not need to upgrade the
firmware.

• Different from the current version (from step 3), follow the steps below to
download and upgrade the firmware.

7. Insert a USB drive into the USB port on the computer to download the new firm-
ware to the USB drive.

60 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Chapter 5 Utilities
Viewing ProFlex™Instrument statistics (PLEASE CONFIRM CONTENT) 5

8. Click the link in the Software column to download the file. Select the USB drive
as the location for the saved file.

9. Remove the USB drive from the computer when the download is complete.

To upgrade the ProFlex™ Instrument with the new firmware:

1. Follow the instructions in “Backing Up Run Methods and Other Settings” on
page 1-13 (insert link later) to save your run methods and settings from the Pro-
Flex™ PCR System.

2. Insert the USB drive with the new firmware in the USB port at the rear end of the
ProFlex™ PCR System.

3. Log in as a user with Administrator or Service access.

4. In the Home screen, touch Settings.

5. In the Settings screen, touch Administrator Settings.

6. In the Administrator Settings screen, touch Upgrade Firmware.

The Firmware Upgrade screen opens: (insert screenshot)

7. Touch Source File to display a list of the files on the USB drive. ProFlex™ System
firmware files end with “.update”.

8. Touch the name of the file with the new firmware, then touch Select.

9. Touch Upgrade, then Yes.

When the upgrade process is complete, a message box opens.

10. Restart the ProFlex™ instrument.

Viewing ProFlex™Instrument statistics (PLEASE CONFIRM

CONTENT)
The instrument records information about how it has been used. You can view this
information.

1. In the Home screen, touch Settings.

2. In the Tools Menu screen, touch Show Statistics.

The following statistics are shown:
• Total Cycles for Block(s) – The total number of cycles the instrument has
performed over its lifetime.
• Total Degrees for Block(s) – The total degrees the block(s) have climbed
over their lifetime. For example, if the sample block(s) were heated from
10°C to 30°C, for 3 cycles, the total degrees would be 60. [3 × (30 − 10) = 60].

3. When you finish, touch Done to return to the Settings screen.

ProFlex™ PCR System User Guide 61

For Research Use Only. Not for use in diagnostic procedures.
5 Chapter 5 Utilities
Restoring factory settings (PLEASE CONFIRM CONTENT)

Restoring factory settings (PLEASE CONFIRM CONTENT)

You can reset the instrument to the default values for all instrument settings. When
you do this, the following are lost:
• All run methods except the predefined methods listed in Appendix D, “Prede-
fined Run Methods”
• Any users and their custom folders
• The instrument name and IP address
• Any changes to time-outs, block idle temperatures, security settings, printer set-
tings, and any shortcuts to methods or functions

1. Log in as a user with Administrator access.

2. In the Home screen, touch Settings.

3. In the Settings screen, touch Device Settings.

4. In the Device Settings screen, touch Restore Factory Settings to open the Restore
Factory Settings screen: (insert screenshot)

5. In the Restore Factor Settings screen, touch Restore.

The Restore Factory Settings dialog box is displayed.

6. In the dialog box, touch:

• Yes to back up run methods and other settings before restoring the default
settings to the instrument. Insert a USB drive for the backup.

• No to restore the default settings, without first performing a back up. Before
the restore operation begins, you are prompted to confirm the restore with a
second dialog box.

IMPORTANT! Touching No erases all run methods, user accounts, and other
settings from the instrument.

• Cancel to cancel the restore operation.

When the restore process is complete, a dialog box stating “Factory settings have
been restored.” opens.

7. Remove the USB drive, if present, then toggle the switch on the back of the in-
strument to power off.

8. Toggle the switch again to power on the instrument and restart with the factory
default settings.

62 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 A Ordering Information

Consumables
Contact your regional Life Technologies sales office to order kits, consumables, and
spare parts for the ProFlex™ System. For more information on obtaining support, refer
to the ProFlex™ PCR SystemUser Guide (Pub. no. MAN0007697).

IMPORTANT! There are no 32-well Microplates available for use with the ProFlex™
3x32-Well PCR System. Instead, single tubes with caps or tube strips with caps or cap
strips are used for running the PCR samples.

IMPORTANT! In Tables 1 and Table 9, the Format column indicates the model(s) of
the ProFlex™ System that are compatible with the associated kit or disposable. Before
ordering, verify that your ProFlex™ System supports the kit or disposable.

Table 9 ProFlex™ PCR SystemConsumables

Consumable Format Part No.

Single Tubes

MicroAmp® Reaction Tube with Cap, 0.2-mL 0.2 (32 N8010540/

Well N8011540
and 96
Well)

MicroAmp® Reaction Tube with Cap, Assorted Colors, 0.2- 0.2 (32 N8010840
mL Well
and 96
Well)

MicroAmp® Reaction Tube with Cap, Autoclaved, 0.2-mL 0.2 (32 N8010612
Well
and 96
Well)

MicroAmp® Reaction Tube without Cap, 0.2-mL 0.2 (32 N8010533/

Well N8011533
and 96
Well)

MicroAmp® Reaction Tube without Cap, Assorted Colors, 0.2 (32 N8010833
0.2-mL Well
and 96
Well)

ProFlex™ PCR System User Guide 63

For Research Use Only. Not for use in diagnostic procedures.
A Appendix A Ordering Information
Consumables

Consumable Format Part No.

MicroAmp® Optical Tube without Cap, 0.2-mL 0.2 (32 N8010933

Well
and 96
Well)

MicroAmp® 96-Well Reaction Tube/Tray/Retainer Set, 0.2- 0.2 (96 403083/ 403086
mL Well)

Reaction Trays

MicroAmp® 96-Well Reaction Tray for VeriFlex™ Systems 0.2 (96 4379983
Well)

MicroAmp® 96-Well Reaction Tray/ Retainer for VeriFlex™ 0.2 (96 4381850
Systems Well)

MicroAmp® 3x32-Well Retainer for VeriFlex™ Systems 0.2 (32 4481669

Well)

Seals and Covers

MicroAmp® Clear Adhesive Film N/A 4306311

MicroAmp® Optical Adhesive Film N/A 4360954/ 4311971

MicroAmp® Optical Adhesive Film Kit N/A 4313663

96-Well Plates

MicroAmp® Optical 96-Well Reaction Plate 0.2 (96 N8010560/

Well) 4316813

MicroAmp® Optical 96-Well Reaction Plate with Barcode 0.2 (96 4306737/ 4326659
Well)

MicroAmp® Optical 96-Well Reaction Plate with Barcode 0.2 (96 403012
and Optical Caps Well)

MicroAmp® Optical 96-Well Reaction Plate with Barcode 0.2 (96 403012
and Optical Adhesive Films Well)

8-Well Strips

MicroAmp® 8-Tube Strip, 0.2-mL 0.2 N8010580

MicroAmp® 8-Tube Strip, Assorted Colors, 0.2-mL 0.2 N8010838

MicroAmp® Optical 8-Tube Strip, 0.2-mL 0.2 4316567

MicroAmp® 8-Cap Strip 0.2 N8011535

MicroAmp® 8-Cap Strip, Assorted Colors 0.2 N8010835

MicroAmp® Optical 8-Cap Strip 0.2 4323032

64 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Appendix A Ordering Information
Consumables A

Table 10 ProFlex™ PCR SystemConsumables Sample Handling Supplies and Spare

Parts

Part Name Part No.

Sample Handling Supplies

MicroAmp®Adhesive Film Applicator 4333183

MicroAmp®Multi-Removal Tool 4313950

MicroAmp®Cap Installing Tool (Handle) 4330015

®
MicroAmp 96-Well Base N8010531

MicroAmp®Splash Free 96-Well Base 4312063

GeneAmp®Thermal Isolation Frame 4308927

MicroAmp®Centrifuge Adapter N8013822

Instrument Parts

Cable, AC Power Supply 4374647

Cam, Left Side 4374274

Cam, Right Side 4374275

Foot, Rubber (Package of 4) 4375246

Fuse, 10-Amp, SLO-BLO 4377690

Grill, Plastic Mold 4374178

Handle, Plastic 4374277

Handle, Rubber Grip 4374181

Heated Cover 4374276

Panel, Left 4374176

Panel, Rear (Heated Cover) 4374280

Panel, Right 4374175

ProFlex™ PCR System User Guide 65

For Research Use Only. Not for use in diagnostic procedures.
 B Troubleshooting

Troubleshooting problems
Use the following table to help troubleshoot problems with your ProFlex™ System.

Problem Possible cause Check and/or remedy

Cooling rate too Peltier assembly failure Run the Cycle Performance Test.
slow

Temperature does Instrument may require Run the Temperature Verification Test.
not match specified calibration
temperature

Heating rate too Peltier failure Run the Cycle Performance Test.
slow

Printing fails • Printer incorrectly • Make sure the printer IP address on

configured on in- the instrument matches the IP ad-
strument dress from the system administra-
• Printer not connec- tor.
ted to network • Is Ethernet cable connected to the
• Instrument not printer?
connected to net- • Is Ethernet cable connected to the
work instrument?
• Ethernet cable bad • Is network functioning?
• Try a different Ethernet cable.

Cycling time too Peltier assembly failure Run the Cycle Performance Test.
long

When running on • Instrument(s) not • Are all Ethernet cables connected to

multiple instru- connected to net- the instruments?
ments, cannot see work • Are all Ethernet cables connected to
instruments in “Se- • Switch powered off the network switch?
lect Instruments”
• Ethernet cable bad • Try a different Ethernet cable.
screen
• IP address conflict • Change the IP addresses on the in-
• Allow Remote Runs struments.
setting in Instru- • In Instrument Name screen, change
ment Name screen Allow Remote Runs to Yes.
set to No.

66 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Appendix B Troubleshooting
Returning an instrument for service B

Problem Possible cause Check and/or remedy

No screen display Blown fuse Check fuses.

No response when Not connected to power Is power switch on?
you power on the source
Is power cord connected?
instrument

Screen display is LCD screen failure Request service.

missing characters
or is illegible

Sample tubes melt Incompatible consuma- Use recommended consumables (see

during run bles used Appendix A, “Ordering Information”).
Heated cover too hot Run the Heated Cover Verification Test.
Sample block(s) too hot

Heating cover stuck Cam, platen, or cover Request service

assembly malfunction-
ing

Heated cover not Heated cover failure Run the Heated Cover Verification Test.
responsive

Cannot see Manage Manage Users button is • Use VeritiLink Software to manage
Users button in Ad- not shown when instru- user accounts
ministrator Settings ment is monitored by or
screen VeritiLink™ Remote • Change Allow VeritiLink Software
Management Software Control to No in the Security screen
on the instrument.

Returning an instrument for service

Before returning an instrument for service, back up the run methods and instrument
settings, as described in “Backing Up Run Methods and Other Settings” on page 1-13
(insert link later). This practice ensures that if the service center sends you a replace-
ment instrument, you can restore the run methods and other settings.
To return an instrument for service:

1. Contact your local customer care center or technical support group to obtain a
copy of the Certificate of Instrument Decontamination, a service notification, a
service call number, and, if required, packaging.

2. Decontaminate the instrument.

IMPORTANT! If the sample block(s) become contaminated with radioactivity,

use a commercially available decontaminant to remove the contamination. If the
block(s) cannot be decontaminated, the instrument cannot be returned to Life
Technologies for service.

3. Complete and sign a copy of the Certificate of Instrument Decontamination.

ProFlex™ PCR System User Guide 67

For Research Use Only. Not for use in diagnostic procedures.
B Appendix B Troubleshooting
Returning an instrument for service

4. Fax the Certificate of Instrument Decontamination to the customer care center.

5. Pack the instrument in the provided packaging, without any accessories or pow-
er cords. Include a hard copy of the Certificate of Instrument Decontamination in
the box.
Note: Repairs for instruments without the Certificate of Instrument Decontami-
nation are delayed.

6. Affix the provided postage to the box, then ship the instrument to the designated
facility.
The repair process requires 2 to 3 weeks.

68 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 C Instrument specifications

This chapter lists the instrument specifications for the ProFlex™ PCR System using the
ProFlex™ 96-Well Sample Block and the ProFlex™ 3x32-Well Sample Block.

System specifications
The following table displays the system specifications for the ProFlex™ PCR System
using the ProFlex™ 96-Well Sample Block and the ProFlex™ 3x32-Well Sample Block.

Table 11 ProFlex™ PCR System technical specifications

Block format 3 x 32, 0.2 mL inde- 96-well, 0.2 mL VeriFlex™ block

pendent control

Maximum block ramp rate 6.00°C/sec

Maximum sample ramp 4.40°C/sec

rate

Temperature accuracy ±0.25°C (35 to 99.9°C)

Temperature range for pro- 0°C to 100.0°C

tocol run

Temperature uniformity <0.5°C (30 seconds after reaching 95°C)

Dimensions Height: 27.2 cm (10.7 in) Width: 33.0 cm (13 in) Depth: 56.5
cm (22 in)

Weight 18.75 kg (41 lb)

PCR volume range 10 - 80 µL

Instrument memory USB, on-board

Display interface 8.4" color TFT LCD

Power 100-240 V, 50-60 Hz Max: 950 VA

VeriFlex™ blocks 5°C, 2 VeriFlex™ zones 25°C (5°C zone-to-zone), 6 Veri-

Flex™ zones

Ambient humidity 15% - 80% Relative Humidity, non-condensing (acceptable

range)

Ambient operating temper- 15°C to 30°C (acceptable range)

ature

ProFlex™ PCR System User Guide 69

For Research Use Only. Not for use in diagnostic procedures.
C Appendix C Instrument specifications
Location of power point and ports on the instrument

Table 12 Power and communication ports

Port Description

800 VA power cable port

10/100 Fast Ethernet port for connecting to a network

USB v1.0 port for connecting to an external network drive, jump

drive, or other USB storage device

RS232 serial communication port for connecting the probe for the
Temperature Verification Kit

USB v1.0 port for connecting to USB-enabled Wi-Fi Card

Location of power point and ports on the instrument

The following graphic displays the location of the power cord insertion point as well
as the various ports provided in the ProFlex™ instrument.

70 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 D Predefined Run Methods

The following run methods are supplied with the ProFlex™ PCR System. These run
methods are based on recommended protocols from Life Technologies and should be
considered as starting points for the different applications. Your results may vary be-
tween instrument models, so some optimization may be needed. Please note that the
ramp rates of these methods are 100%.
• AccuPrime PFX 2-Step Method
• AccuPrime PFX 3-Step Method
• AccuPrime Taq DNA Polymerase Method
• AccuPrime Taq High Fidelity Method
• AmpliTaq 360 Method
• AmpliTaq Gold® Fast PCR Run Method
• AmpliTaq Gold® Method
• AmpliTaq®Method
• Basic PCR Taq Method
• BigDye® Direct Cycle Sequence Method
• BigDye® Direct PCR Run Method
• BigDye® Kit Fast Run Method
• BigDye® Kit Standard Run Method
• Elongase 2-Step Method
• Elongase 3-Step Method
• General PCR Run Method
• Platinium PFX 2-Step Method
• Platinium PFX 3-Step Method
• Platinium Taq DNA Polymerase Method
• Platinium Taq High Fidelity Method
• Platinium Taq SuperMix Method
• SuperScript III 1-Step System Method
• TargetSeq Exome Enrichment Method
• Time Release Run Method
• Touchdown PCR Run Method
These run methods cannot be edited or deleted, but if you save them with a different
name or to a different folder, you can edit them.

ProFlex™ PCR System User Guide 71

For Research Use Only. Not for use in diagnostic procedures.
D Appendix D Predefined Run Methods
AmpliTaq Gold® Fast PCR run method

AmpliTaq Gold® Fast PCR run method

Use this run method for PCR with AmpliTaq Gold® Fast PCR Master Mix, UP to pro-
duce optimal yield and specificity for a 500 bp amplicon. This run method is opti-
mized to generate template for DNA sequencing reactions and is only available on
ProFlex™ 96-Well PCR System.

Note: By default, the annealing temperature is set at 62°C. Replace this temperature
with the melting temperature of the primer.

BigDye® Kit Fast run method

Use this run method for cycle sequencing of dideoxy (Sanger) terminator sequencing
using ABI Prism® BigDye® Terminator Cycle Sequence Ready Reaction Kits.

BigDye® Kit Standard Run Method

Use this run method, which consists of cycle-sequencing parameters for dideoxy
(Sanger) terminator sequencing, with ABI Prism® BigDye® Terminator Cycle Sequenc-
ing Ready Reaction Kits.

72 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Appendix D Predefined Run Methods
General PCR run method D

The pre-coded conditions in this run method are optimized for AmpliTaq® DNA Pol-
ymerase, FS and the cycle sequencing reagents supplied with the BigDye® Terminator
kits.

General PCR run method

Use this run method for standard PCR use. This method is set as the default run
method for the ProFlex™ PCR System.

Time Release run method

Use this run method for use with AmpliTaq Gold® DNA Polymerase. This method
minimizes the pre-PCR activation step and adds a minimum of 10 additional cycles,
allowing for slow activation of the enzyme during cycling. This provides a simple
method where polymerase activity increases more slowly as product accumulates, im-
proving specificity.

ProFlex™ PCR System User Guide 73

For Research Use Only. Not for use in diagnostic procedures.
D Appendix D Predefined Run Methods
Touchdown PCR run method

Touchdown PCR run method

Use this run method if the optimal annealing temperature is not known. This method
incrementally decreases the annealing temperature in early cycles to maximize the
yield of specific products.

74 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 E Safety

WARNING! GENERAL SAFETY. Using this product in a manner not specified

in the user documentation may result in personal injury or damage to the in-
strument or device. Ensure that anyone using this product has received instruc-
tions in general safety practices for laboratories and the safety information pro-
vided in this document.
· Before using an instrument or device, read and understand the safety infor-
mation provided in the user documentation provided by the manufacturer of
the instrument or device.
· Before handling chemicals, read and understand all applicable Safety Data
Sheets (SDSs) and use appropriate personal protective equipment (gloves,
gowns, eye protection, etc). To obtain SDSs, see the “Documentation and
Support” section in this document.

Symbols on this instrument

Symbols may be found on the instrument to warn against potential hazards or convey
important safety information. In this document, the hazard symbol is used along with
one of the following user attention words:
• CAUTION! – Indicates a potentially hazardous situation that, if not avoided,
may result in minor or moderate injury. It may also be used to alert against un-
safe practices.
• WARNING! – Indicates a potentially hazardous situation that, if not avoided,
could result in death or serious injury.
• DANGER! – Indicates an imminently hazardous situation that, if not avoided,
will result in death or serious injury.

Symbol English Français

Caution, risk of danger Attention, risque de danger

Consult the manual for further safety Consulter le manuel pour d’autres
information. renseignements de sécurité.

Caution, hot surface Attention, surface chaude

Caution, risk of electrical shock Attention, risque de choc électrique

Potential biohazard Danger biologique potentiel

ProFlex™ PCR System User Guide 75

For Research Use Only. Not for use in diagnostic procedures.
E Appendix E Safety
Symbols on this instrument

Symbol English Français

Potential slipping hazard Danger de glisser potentiel

On On (marche)

Off Off (arrêt)

On/Off On/Off (marche/arrêt)

Standby En attente

Earth (ground) terminal Borne de (mise à la) terre

Protective conductor terminal (main Borne de conducteur de protection

ground) (mise à la terre principale)

Terminal that can receive or supply Borne pouvant recevoir ou envoyer

alternating current or voltage une tension ou un courant de type al-
ternatif

Terminal that can receive or supply Borne pouvant recevoir ou envoyer

alternating or direct current or volt- une tension ou un courant continu ou
age alternatif

Do not dispose of this product in un- Ne pas éliminer ce produit avec les
sorted municipal waste déchets usuels non soumis au tri sé-
lectif.
CAUTION! To minimize neg-
ative environmental impact CAUTION! Pour minimiser
from disposal of electronic les conséquences négatives
waste, do not dispose of elec- sur l’environnement à la suite
tronic waste in unsorted mu- de l’élimination de déchets
nicipal waste. Follow local électroniques, ne pas élimin-
municipal waste ordinances er ce déchet électronique
for proper disposal provision avec les déchets usuels non
and contact customer service soumis au tri sélectif. Se con-
for information about respon- former aux ordonnances lo-
sible disposal options. cales sur les déchets munici-
paux pour les dispositions
d’élimination et communi-
quer avec le service à la cli-
entèle pour des renseigne-
ments sur les options d’élimi-
nation responsable.

76 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Appendix E Safety
Safety alerts on this instrument E

Conformity
symbols
Safety alerts on this instrument
Additional text may be used with one of the symbols described above when more
specific information is needed to avoid exposure to a hazard. See the following table
for safety alerts found on the instrument.

English French translation

ATTENTION! Produits chimiques danger-

CAUTION! Hazardous chemicals.
eux. Lire les fiches signalétiques (FS) avant
Read the Safety Data Sheets (SDSs)
de manipuler les produits.
before handling.

ATTENTION! Déchets dangereux. Lire les

CAUTION! Hazardous waste. Refer
fiches signalétiques (FS) et la réglementa-
to SDS(s) and local regulations for
tion locale associées à la manipulation et à
handling and disposal.
l’élimination des déchets.

Location of safety
labels on this
instrument

ProFlex™ PCR System User Guide 77

For Research Use Only. Not for use in diagnostic procedures.
E Appendix E Safety
Safety alerts on this instrument

78 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Appendix E Safety
Safety information for instruments not manufactured by Life Technologies E

Safety information for instruments not manufactured by Life

Technologies
Some of the accessories provided as part of the instrument system are not designed or
built by Life Technologies. Consult the manufacturer's documentation for the infor-
mation needed for the safe use of these products.

Instrument safety

General CAUTION! Do not remove instrument protective covers. If you remove the
protective instrument panels or disable interlock devices, you may be exposed
to serious hazards including, but not limited to, severe electrical shock, laser ex-
posure, crushing, or chemical exposure.

z_Physical CAUTION! Moving Parts. Moving parts can crush, pinch and cut. Keep hands
Injury_ProFlex clear of moving parts while operating the instrument. Disconnect power before
servicing.

WARNING! Sharp Edges. Watch your fingers.

Electrical WARNING! Ensure appropriate electrical supply. For safe operation of the in-
strument:
· Plug the system into a properly grounded receptacle with adequate current
capacity.
· Ensure the electrical supply is of suitable voltage.
· Never operate the instrument with the ground disconnected. Grounding con-
tinuity is required for safe operation of the instrument.

WARNING! Power Supply Line Cords. Use properly configured and approved
line cords for the power supply in your facility.

WARNING! Disconnecting Power. To fully disconnect power either detach or

unplug the power cord, positioning the instrument such that the power cord is
accessible.

ProFlex™ PCR System User Guide 79

For Research Use Only. Not for use in diagnostic procedures.
E Appendix E Safety
Safety and electromagnetic compatibility (EMC) standards

Cleaning and CAUTION! Cleaning and Decontamination. Use only the cleaning and decon-
decontamination tamination methods specified in the manufacturer's user documentation. It is
the responsibility of the operator (or other responsible person) to ensure the fol-
lowing requirements are met:
· No decontamination or cleaning agents are used that could cause a HAZ-
ARD as a result of a reaction with parts of the equipment or with material
contained in the equipment.
· The instrument is properly decontaminated a) if hazardous material is spil-
led onto or into the equipment, and/or b) prior to having the instrument
serviced at your facility or sending the instrument for repair, maintenance,
trade-in, disposal, or termination of a loan (decontamination forms may be
requested from customer service).
· Before using any cleaning or decontamination methods (except those recom-
mended by the manufacturer), users should confirm with the manufacturer
that the proposed method will not damage the equipment

Laser CAUTION! LASER HAZARD, Bar Code Scanner. The bar code scanner inclu-
ded with the instrument system is a Class 2 laser. To avoid damage to eyes, do
not stare directly into the beam or point into another person's eyes.

Safety and electromagnetic compatibility (EMC) standards

The instrument design and manufacture complies with the standards and require-
ments for safety and electromagnetic compatibility as noted in the following table:

Safety Reference Description

EU Directive 2006/95/EC European Union “Low Voltage Directive”

IEC 61010-1 Safety requirements for electrical equip-

ment for measurement, control, and labo-
EN 61010-1
ratory use – Part 1: General requirements
UL 61010-1
CSA C22.2 No. 61010-1

IEC 61010-2-010 Safety requirements for electrical equip-

ment for measurement, control and labora-
EN 61010-2-010
tory use – Part 2-010: Particular require-
ments for laboratory equipment for the
heating of materials

IEC 61010-2-081 Safety requirements for electrical equip-

ment for measurement, control and labora-
EN 61010-2-081 tory use – Part 2-081: Particular require-
ments for automatic and semi-automatic
laboratory equipment for analysis and other
purposes

IEC 60825-1 Safety of laser products – Part 1: Equip-

ment classification and requirements
EN 60825-1

80 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Appendix E Safety
Safety and electromagnetic compatibility (EMC) standards E

Reference Description

21 CFR 1040.10 and 1040.11 as applicable U.S. FDA Health and Human Services (HHS)
“Radiological health performance stand-
ards for laser products” and “Radiological
health performance standards for specific
purpose laser products”

EMC Reference Description

Directive 2004/108/EC European Union “EMC Directive”

EN 61326-1 Electrical Equipment for Measurement,

Control and Laboratory Use – EMC Re-
quirements – Part 1: General Requirements

FCC Part 18 (47 CFR) U.S. Standard “Industrial, Scientific, and

Medical Equipment”

AS/NZS 2064 Limits and Methods of Measurement of

Electromagnetic Disturbance Characteris-
tics of Industrial, Scientific, and Medical
(ISM) Radiofrequency Equipment

ICES-001, Issue 3 Industrial, Scientific and Medical (ISM) Ra-

dio Frequency Generators

Environmental Reference Description

design
Directive 2002/96/EC European Union “WEEE Directive” – Waste
electrical and electronic equipment

Directive 2002/95/EC European Union “RoHS Directive” – Re-

striction of hazardous substances in elec-
trical and electronic equipment

Directive 2006/66/EC European Union “Battery Directive”

MII Order #39 PRC “Management Methods for Controlling

Pollution by Electronic Information Prod-
ucts”

ProFlex™ PCR System User Guide 81

For Research Use Only. Not for use in diagnostic procedures.
E Appendix E Safety
Chemical safety

Chemical safety
WARNING! GENERAL CHEMICAL HANDLING. To minimize hazards, en-
sure laboratory personnel read and practice the general safety guidelines for
chemical usage, storage, and waste provided below, and consult the relevant
SDS for specific precautions and instructions:
· Read and understand the Safety Data Sheets (SDSs) provided by the chemi-
cal manufacturer before you store, handle, or work with any chemicals or
hazardous materials. To obtain SDSs, see the “Documentation and Support”
section in this document.
· Minimize contact with chemicals. Wear appropriate personal protective
equipment when handling chemicals (for example, safety glasses, gloves, or
protective clothing).
· Minimize the inhalation of chemicals. Do not leave chemical containers open.
Use only with adequate ventilation (for example, fume hood).
· Check regularly for chemical leaks or spills. If a leak or spill occurs, follow
the manufacturer's cleanup procedures as recommended in the SDS.
· Handle chemical wastes in a fume hood.
· Ensure use of primary and secondary waste containers. (A primary waste
container holds the immediate waste. A secondary container contains spills
or leaks from the primary container. Both containers must be compatible
with the waste material and meet federal, state, and local requirements for
container storage.)
· After emptying a waste container, seal it with the cap provided.
· Characterize (by analysis if necessary) the waste generated by the particular
applications, reagents, and substrates used in your laboratory.
· Ensure that the waste is stored, transferred, transported, and disposed of ac-
cording to all local, state/provincial, and/or national regulations.
· IMPORTANT! Radioactive or biohazardous materials may require special
handling, and disposal limitations may apply.

82 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Appendix E Safety
Biological hazard safety E

Biological hazard safety

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, in-
fectious agents, and blood of humans and other animals have the potential to
transmit infectious diseases. All work should be conducted in properly equip-
ped facilities using the appropriate safety equipment (for example, physical
containment devices). Safety equipment also may include items for personal
protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face
shields, safety glasses, or goggles. Individuals should be trained according to
applicable regulatory and company/ institution requirements before working
with potentially biohazardous materials. Follow all applicable local, state/pro-
vincial, and/or national regulations. The following references provide general
guidelines when handling biological samples in laboratory environment.
· U.S. Department of Health and Human Services, Biosafety in Microbiological
and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC)
21-1112, Revised December 2009; found at:
www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
· World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/
CDS/CSR/LYO/2004.11; found at:
www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf

ProFlex™ PCR System User Guide 83

For Research Use Only. Not for use in diagnostic procedures.
 Documentation and Support

Related documentation
The following related documents are shipped with the system:

Document Publication num- Description

ber

ProFlex™ PCR System In- 4483807 Describes the procedures for instal-
stallation and Operation ling and using the ProFlex™ PCR Sys-
Quick Reference tem that is described in detail in the
ProFlex™ PCR System User Guide.

ProFlex™ PCR System Sys- 4483803 Provides the reference for accessing
tem Documentation user documentation from the product
website and contact details for service
and technical support.

Note: For additional documentation, see “Obtaining support” on page 84.

Obtaining SDSs
Safety Data Sheets (SDSs) are available from www.lifetechnologies.com/support
Note: For the SDSs of chemicals not distributed by Life Technologies, contact the
chemical manufacturer.

Obtaining Certificates of Analysis

The Certificate of Analysis provides detailed quality control and product qualification
information for each product. Certificates of Analysis are available on our website. Go
to www.lifetechnologies.com/support and search for the Certificate of Analysis by
product lot number, which is printed on the box.

Obtaining support
For the latest services and support information for all locations, go to:
www.lifetechnologies.com/support

84 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Documentation and Support
Limited product warranty

At the website, you can:

• Access worldwide telephone and fax numbers to contact Technical Support and
Sales facilities
• Search through frequently asked questions (FAQs)
• Submit a question directly to Technical Support ([email protected])
• Search for user documents, SDSs, vector maps and sequences, application notes,
formulations, handbooks, certificates of analysis, citations, and other product
support documents
• Obtain information about customer training
• Download software updates and patches

Limited product warranty

Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth
in the Life Technologies' General Terms and Conditions of Sale found on Life Tech-
nologies' website at www.lifetechnologies.com/termsandconditions. If you have any
questions, please contact Life Technologies at www.lifetechnologies.com/support.

ProFlex™ PCR System User Guide 85

For Research Use Only. Not for use in diagnostic procedures.
 Glossary

86 ProFlex™ PCR System User Guide

For Research Use Only. Not for use in diagnostic procedures.
 Index

B R
biohazard safety 83 related documentation 84

C S
Certificates of Analysis, obtaining 84 safety
biohazard 83
Safety Data Sheets (SDSs), obtaining 84
D support, obtaining 84
documentation, related 84 symbols, safety 75

I T
Index Term 8, 9, 14, 21, 22, 24, 27, 29–32, 36, 37, 48, terms and conditions 85
49, 51–53, 56–62, 70 training, information on 84

L W
limited product warranty 85 warranty 85

M
Material Data Safety Sheets (MSDSs). See Safety Data
Sheets (SDSs)

ProFlex™ PCR System User Guide 87

For Research Use Only. Not for use in diagnostic procedures.
Headquarters
5791 Van Allen Way | Carlsbad, CA 92008 USA | Phone +1 760 603 7200 | Toll Free in USA 800 955 6288
For support visit lifetechnologies.com/support or email [email protected]
lifetechnologies.com

30 April 2013