APTT
APTT
APTT
CONTENTS
APTT
Activated Partial Thromboplastin Time
REF 3510201 APTT 10 x 4 mL
(APTT)
Ellagic acid
For in vitro diagnostic use only
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REFERENCE VALUES
APTT results are influenced by the method of clot detection and - Traceability: Stago PTTA (micronised silica activator)
can vary from laboratory to laboratory. In general an APTT test
performed on a photo-optical coagulometer will give clotting time for - Accuracy
normal plasma in the range of 25 to 43 seconds. Therapeutic Results obtained with this reagent did not show significative
ranges for monitoring oral anticoagulation therapy will vary from differences when compared with reference reagents. Details of
laboratory to laboratory, therefore it is essential that each laboratory the comparison experiments are available on request.
establish relevant APTT ranges for its respective patient population.
Abnormal results obtained with a plasma from a patient not on REFERENCES
anticoagulant therapy may indicate a factor deficiency or the 1. Human Blood Coagulation, Hemostasis and Thrombosis, 3rd ed.
presence on an inhibitor. The result may also be due to the effects R Biggs, CR Rizza, Editors, Blackwell Scientific Publications,
of certain drugs and medications. Additional procedures such as London (1984).
the PT test and mixing studies using factor deficient plasma are 2. Cole E, Hall ER, Wu KK, Principles of Antithromboltic Therapy. In
usually required. Wu KK, Thomboembolic Disorders, PSG Publishing Co. Inc.,
Litteton, p 91 (1984).
QUALITY CONTROL 3. Triplett DA, Heparin: Clinical use and Laboratory Monitoring. In
Triplett DA, Laboratory Evaluation of Coagulation, American
Control sera are recommended to monitor the performance of Society of Clinical Pathologists Press, Chicago p 272 (1982).
assay procedures. They should be used as sample. 4. Hougie C, The Biochemistry of Blood Coagulation, In Laboratory
Evaluation of Coagulation, American Society of Clinical
REF 3520101 PLASMA CONTROL LEVEL 1
Pathologists Press, Chicago p 2 (1982).
REF 3520201 PLASMA CONTROL LEVEL 2 5. Owen CA, Bowie EJW, Thomson JH, The Diagnosis of Bleeding
Disorders, Little Brown and Company, Boston p 110 (1975).
6. Harker LA, Hemostasis Manual, FA Davis Co, Philadelphia p 62
Each laboratory should establish its own Quality Control scheme
(1974).
and corrective actions if controls do not meet the acceptable
7. Triplett DA, Harms CS, Procedures for the Coagulation
tolerances. Laboratory, American Society of Clinical Pathologists Press,
Chicago, p 7 (1981).
CLINICAL SIGNIFICANCE 8. Young DS, Pestaner LC, Gibberman V, Effects of Drugs on
Clinical Laboratory Tests, Clin Chem 21; 1D (1975).
The APTT reagent is an in vitro diagnostic assay intended for use in
determining activated partial thromboplastin time (APTT) and
coagulation factor assays that are based on a modified APTT.
The activated partial thromboplastin time (APTT) is used as a
general screening test for the detection of coagulation abnormalities
in the intrinsic pathway. The APTT is sensitive to deficiencies or
abnormalities of factors VIII, IX, XI, XII, X, and II, prekallikrein, high
molecular weight kininogen (HMWK), and fibrinogen. APTT is also
sensitive to inhibitors of blood coagulation such as lupus inhibitor
and fibrin/fibrinogen degradation products (1). The APTT is the most
widely used method for monitoring intravenous heparin
anticoagulation therapy2, 3.
ANALYTICAL PERFORMANCE
- Linearity
0.2-0.6 IU Unfractionated heparin
0.01-0.65 IU Factor VIII
- Analytical sensitivity
< 0. 01 IU Factor VIII
- Heparin sensitivity:
The anticoagulant action of heparin depends on many factors. Each
laboratory should determine the relative heparin sensitivity by
adding known amounts of unfractionated heparin to pooled normal
plasma and determining the elevation in clotting time using the
APTT reagent.
C35102-3/1202
R1.ing
QUALITY SYSTEM CERTIFIED LINEAR CHEMICALS, S.L.U. Joaquim Costa 18 2ª planta. 08390 Montgat (Barcelona) SPAIN
ISO 9001 ISO 13485 Telf. (+34) 934 694 990; E-mail: [email protected] ; website: www.linear.es NIF-VAT:B60485687