SAFETY DATA SHEET Version 6.

10
Revision Date 09/06/2024
Print Date 09/06/2024

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product identifiers
Product name : Cycloheximide
Product Number : C7698
Brand : Sigma
Index-No. : 613-140-00-8
CAS-No. : 66-81-9
1.2 Relevant identified uses of the substance or mixture and uses advised against
Identified uses : Laboratory chemicals, Synthesis of substances

Uses advised against : The product is being supplied under the TSCA R&D Exemption
(40 CFR Section 720.36). It is the recipient’s responsibility to
comply with the requirements of the R&D exemption. The
product may not be used for a non-exempt commercial purpose
under TSCA unless appropriate consent is granted in writing by
MilliporeSigma.
1.3 Details of the supplier of the safety data sheet
Company : Sigma-Aldrich Inc.
3050 SPRUCE ST
ST. LOUIS MO 63103
UNITED STATES
Telephone : +1 314 771-5765
Fax : +1 800 325-5052
1.4 Emergency telephone
Emergency Phone # : 800-424-9300 CHEMTREC (USA) +1-703-
527-3887 CHEMTREC (International) 24
Hours/day; 7 Days/week

SECTION 2: Hazards identification

2.1 Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
Acute toxicity, Oral (Category 2), H300
Germ cell mutagenicity (Category 2), H341
Reproductive toxicity (Category 1B), H360
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 Short-term (acute) aquatic hazard (Category 2), H401
Long-term (chronic) aquatic hazard (Category 2), H411
For the full text of the H-Statements mentioned in this Section, see Section 16.
2.2 GHS Label elements, including precautionary statements
Pictogram

Signal Word Danger

Hazard Statements
H300 Fatal if swallowed.
H341 Suspected of causing genetic defects.
H360 May damage fertility or the unborn child.
H411 Toxic to aquatic life with long lasting effects.
Precautionary Statements
P201 Obtain special instructions before use.
P202 Do not handle until all safety precautions have been read and
understood.
P264 Wash skin thoroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P273 Avoid release to the environment.
P280 Wear protective gloves/ protective clothing/ eye protection/ face
protection.
P301 + P310 + P330 IF SWALLOWED: Immediately call a POISON CENTER/ doctor.
Rinse mouth.
P308 + P313 IF exposed or concerned: Get medical advice/ attention.
P391 Collect spillage.
P405 Store locked up.
P501 Dispose of contents/ container to an approved waste disposal
plant.

2.3 Hazards not otherwise classified (HNOC) or not covered by GHS - none

SECTION 3: Composition/information on ingredients

3.1 Substances
Synonyms : Actidione
Naramycin A
3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-
hydroxyethyl]glutarimide

Formula : C15H23NO4
Molecular weight : 281.35 g/mol
CAS-No. : 66-81-9
EC-No. : 200-636-0
Index-No. : 613-140-00-8
Component Classification Concentration

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 Cycloheximide
Acute Tox. 2; Muta. 2; <= 100 %
Repr. 1B; Aquatic Acute 2;
Aquatic Chronic 2; H300,
H341, H360, H401, H411

For the full text of the H-Statements mentioned in this Section, see Section 16.

SECTION 4: First aid measures

4.1 Description of first-aid measures
General advice
Show this material safety data sheet to the doctor in attendance.
If inhaled
After inhalation: fresh air. Call in physician.
In case of skin contact
In case of skin contact: Take off immediately all contaminated clothing. Rinse skin with
water/ shower. Consult a physician.
In case of eye contact
After eye contact: rinse out with plenty of water. Call in ophthalmologist. Remove contact
lenses.
If swallowed
If swallowed: give water to drink (two glasses at most). Seek medical advice immediately.
In exceptional cases only, if medical care is not available within one hour, induce vomiting
(only in persons who are wide awake and fully conscious), administer activated charcoal
(20 - 40 g in a 10% slurry) and consult a doctor as quickly as possible.
4.2 Most important symptoms and effects, both acute and delayed
The most important known symptoms and effects are described in the labelling (see section
2.2) and/or in section 11
4.3 Indication of any immediate medical attention and special treatment needed
No data available

SECTION 5: Firefighting measures

5.1 Extinguishing media
Suitable extinguishing media
Water Foam Carbon dioxide (CO2) Dry powder
Unsuitable extinguishing media
For this substance/mixture no limitations of extinguishing agents are given.
5.2 Special hazards arising from the substance or mixture
Carbon oxides
Nitrogen oxides (NOx)
Combustible.
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 Development of hazardous combustion gases or vapours possible in the event of fire.
5.3 Advice for firefighters
Stay in danger area only with self-contained breathing apparatus. Prevent skin contact by
keeping a safe distance or by wearing suitable protective clothing.
5.4 Further information
Suppress (knock down) gases/vapors/mists with a water spray jet. Prevent fire
extinguishing water from contaminating surface water or the ground water system.

SECTION 6: Accidental release measures

6.1 Personal precautions, protective equipment and emergency procedures
Advice for non-emergency personnel: Avoid inhalation of dusts. Avoid substance contact.
Ensure adequate ventilation. Evacuate the danger area, observe emergency procedures,
consult an expert.
For personal protection see section 8.
6.2 Environmental precautions
Do not let product enter drains.
6.3 Methods and materials for containment and cleaning up
Cover drains. Collect, bind, and pump off spills. Observe possible material restrictions
(see sections 7 and 10). Take up carefully. Dispose of properly. Clean up affected area.
Avoid generation of dusts.
6.4 Reference to other sections
For disposal see section 13.

SECTION 7: Handling and storage

7.1 Precautions for safe handling
Advice on safe handling
Work under hood. Do not inhale substance/mixture.
Hygiene measures
Immediately change contaminated clothing. Apply preventive skin protection. Wash hands
and face after working with substance.
For precautions see section 2.2.
7.2 Conditions for safe storage, including any incompatibilities
Storage conditions
Tightly closed. Dry. Keep in a well-ventilated place. Keep locked up or in an area accessible
only to qualified or authorized persons.
Storage stabilityRecommended storage temperature
2 - 8 °C
Storage class
Storage class (TRGS 510): 6.1A: Combustible, acute toxic Cat. 1 and 2 / very toxic
hazardous materials
7.3 Specific end use(s)
Apart from the uses mentioned in section 1.2 no other specific uses are stipulated
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SECTION 8: Exposure controls/personal protection
8.1 Control parameters
Ingredients with workplace control parameters
Contains no substances with occupational exposure limit values.
8.2 Exposure controls
Appropriate engineering controls
Immediately change contaminated clothing. Apply preventive skin protection. Wash
hands and face after working with substance.
Personal protective equipment
Eye/face protection
Use equipment for eye protection tested and approved under appropriate
government standards such as NIOSH (US) or EN 166(EU). Safety glasses
Skin protection
This recommendation applies only to the product stated in the safety data sheet,
supplied by us and for the designated use. When dissolving in or mixing with other
substances and under conditions deviating from those stated in EN 16523-1 please
contact the supplier of CE-approved gloves (e.g. KCL GmbH, D-36124 Eichenzell,
Internet: www.kcl.de).
Full contact
Material: Nitrile rubber
Minimum layer thickness: 0.11 mm
Break through time: 480 min
Material tested:KCL 741 Dermatril® L
This recommendation applies only to the product stated in the safety data sheet,
supplied by us and for the designated use. When dissolving in or mixing with other
substances and under conditions deviating from those stated in EN 16523-1 please
contact the supplier of CE-approved gloves (e.g. KCL GmbH, D-36124 Eichenzell,
Internet: www.kcl.de).
Splash contact
Material: Nitrile rubber
Minimum layer thickness: 0.11 mm
Break through time: 480 min
Material tested:KCL 741 Dermatril® L
Body Protection
protective clothing
Respiratory protection
Recommended Filter type: Filter type P3
The entrepeneur has to ensure that maintenance, cleaning and testing of respiratory
protective devices are carried out according to the instructions of the producer.
These measures have to be properly documented.
required when dusts are generated.

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 Our recommendations on filtering respiratory protection are based on the following
standards: DIN EN 143, DIN 14387 and other accompanying standards relating to
the used respiratory protection system.

Control of environmental exposure

Do not let product enter drains.

SECTION 9: Physical and chemical properties

9.1 Information on basic physical and chemical properties
a) Appearance Form: powder
Color: beige
b) Odor No data available
c) Odor Threshold No data available
d) pH No data available
e) Melting 110 °C (230 °F)
point/freezing point
f) Initial boiling point No data available
and boiling range
g) Flash point ()No data available
h) Evaporation rate No data available
i) Flammability (solid, No data available
gas)
j) Upper/lower No data available
flammability or
explosive limits
k) Vapor pressure No data available
l) Vapor density No data available
m) Density No data available
Relative density No data available
n) Water solubility slightly soluble
o) Partition coefficient: log Pow: 0.55 - Bioaccumulation is not expected.
n-octanol/water
p) Autoignition No data available
temperature
q) Decomposition No data available
temperature
r) Viscosity No data available
s) Explosive properties No data available
t) Oxidizing properties No data available

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9.2 Other safety information
No data available

SECTION 10: Stability and reactivity

10.1 Reactivity
The following applies in general to flammable organic substances and mixtures: in
correspondingly fine distribution, when whirled up a dust explosion potential may generally
be assumed.
10.2 Chemical stability
The product is chemically stable under standard ambient conditions (room temperature) .
10.3 Possibility of hazardous reactions
Violent reactions possible with:
Strong oxidizing agents
Strong bases
Acid chlorides
Acid anhydrides

10.4 Conditions to avoid

no information available
10.5 Incompatible materials
No data available
10.6 Hazardous decomposition products
In the event of fire: see section 5

SECTION 11: Toxicological information

11.1 Information on toxicological effects
Acute toxicity
Acute toxicity estimate Oral - 5.1 mg/kg
(Expert judgment)
Remarks: (Regulation (EC) No 1272/2008, Annex VI)
Inhalation: No data available
Dermal: No data available
No data available
Skin corrosion/irritation
Skin - Rabbit
Result: slight irritation
Remarks: (RTECS)
(Regulation (EC) No 1272/2008, Annex VI)
Serious eye damage/eye irritation
Remarks: No data available

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 Respiratory or skin sensitization
Prolonged or repeated exposure may cause allergic reactions in certain sensitive
individuals.
Germ cell mutagenicity
Suspected of causing genetic defects.
Carcinogenicity
IARC: No ingredient of this product present at levels greater than or equal to 0.1% is
identified as probable, possible or confirmed human carcinogen by IARC.
NTP: No ingredient of this product present at levels greater than or equal to 0.1% is
identified as a known or anticipated carcinogen by NTP.
OSHA: No component of this product present at levels greater than or equal to 0.1% is
on OSHA’s list of regulated carcinogens.
Reproductive toxicity
May damage the unborn child.
Specific target organ toxicity - single exposure
No data available
Specific target organ toxicity - repeated exposure
No data available
Aspiration hazard
No data available
11.2 Additional Information
RTECS: MA4375000
To the best of our knowledge, the chemical, physical, and toxicological properties have not
been thoroughly investigated.
After absorption:

We have no description of any toxic symptoms.

Other dangerous properties can not be excluded.

This substance should be handled with particular care.

Liver - Irregularities - Based on Human Evidence

Liver - Irregularities - Based on Human Evidence

SECTION 12: Ecological information

12.1 Toxicity
No data available
(Regulation (EC) No 1272/2008, Annex VI)

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12.2 Persistence and degradability
No data available
12.3 Bioaccumulative potential
No data available
12.4 Mobility in soil
No data available
12.5 Results of PBT and vPvB assessment
PBT/vPvB assessment not available as chemical safety assessment not required/not
conducted
12.6 Endocrine disrupting properties
No data available
12.7 Other adverse effects
No data available

SECTION 13: Disposal considerations

13.1 Waste treatment methods
Product
Waste material must be disposed of in accordance with the national and local regulations.
Leave chemicals in original containers. No mixing with other waste. Handle uncleaned
containers like the product itself.

SECTION 14: Transport information

DOT (US)
UN number: 2811 Class: 6.1 Packing group: II
Proper shipping name: Toxic solids, organic, n.o.s. (Cycloheximide)
Reportable Quantity (RQ):
Poison Inhalation Hazard: No

IMDG
UN number: 2811 Class: 6.1 Packing group: II EMS-No: F-A, S-A
Proper shipping name: TOXIC SOLID, ORGANIC, N.O.S. (Cycloheximide)
Marine pollutant : yes

IATA
UN number: 2811 Class: 6.1 Packing group: II
Proper shipping name: Toxic solid, organic, n.o.s. (Cycloheximide)

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SECTION 15: Regulatory information
CERCLA Reportable Quantity
This material does not contain any components with a CERCLA RQ.
SARA 304 Extremely Hazardous Substances Reportable Quantity
Components CAS-No. Component Calculated product
RQ (lbs) RQ (lbs)
Cycloheximide 66-81-9 100 100
SARA 302 Extremely Hazardous Substances Threshold Planning Quantity
Components CAS-No. Component TPQ (lbs)
Cycloheximide 66-81-9 10000
Cycloheximide 66-81-9 100*
*: Solid in the molten or powdered form (particles < 100 microns), in solution, or meeting
the NFPA reactivity criteria

SARA 311/312 : Acute Health Hazard

Hazards Chronic Health Hazard

SARA 313 : This material does not contain any chemical

components with known CAS numbers that exceed the
threshold (De Minimis) reporting levels established by
SARA Title III, Section 313.

US State Regulations
Massachusetts Right To Know
Cycloheximide 66-81-9
Pennsylvania Right To Know
Cycloheximide 66-81-9
Maine Chemicals of High Concern
Product does not contain any listed chemicals
Vermont Chemicals of High Concern
Product does not contain any listed chemicals
Washington Chemicals of High Concern
Product does not contain any listed chemicals
California Prop. 65
WARNING: This product can expose you to chemicals including Cycloheximide, which is/are
known to the State of California to cause birth defects or other reproductive harm. For more
information go to www.P65Warnings.ca.gov.
The ingredients of this product are reported in the following inventories:
TSCA : Product contains substance(s) not listed on TSCA
inventory.

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 TSCA list
No substances are subject to a Significant New Use Rule.

No substances are subject to TSCA 12(b) export notification requirements.

SECTION 16: Other information

Further information
The information is believed to be correct but is not exhaustive and will be used solely as
a guideline, which is based on current knowledge of the chemical substance or mixture
and is applicable to appropriate safety precautions for the product. It does not represent
any guarantee of the properties of the product. Sigma-Aldrich Corporation and its
Affiliates shall not be held liable for any damage resulting from handling or from contact
with the above product. See www.sigma-aldrich.com and/or the reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2020 Sigma-Aldrich Co. LLC. License granted to make unlimited paper copies
for internal use only.
The branding on the header and/or footer of this document may temporarily not visually
match the product purchased as we transition our branding. However, all of the
information in the document regarding the product remains unchanged and matches the
product ordered. For further information please contact [email protected].
Version: 6.10 Revision Date: 09/06/2024 Print Date: 09/06/2024

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