CLASS 8 HOLIDAY HOMEWORK - BIOLOGy
CLASS 8 HOLIDAY HOMEWORK - BIOLOGy
CLASS 8 HOLIDAY HOMEWORK - BIOLOGy
YESHWANRTHPUR
BANGALORE
LABORATORY MANUAL
BIOLOGY
CLASS- 8
1
LIST OF EXPERIMENTS
1 ASCENT OF SAP 3
2 GIRDLING EXPERIMENT 4
3 OSMOSIS 5
4 TRANSPIRATION 6
2
EXPERIMENT NO 1
ASCENT OF SAP
Procedure :
1. Take a cut shoot (a piece of stem with leaves) of balsam plant and dip it in diluted eosin in
a flask. Leave it for some time.
2. Take sections from different parts of the stem, make slides and examine the sections
under a microscope.
Observation :
1. Red colour is observed in the stem section.
2. The red coloured area of the stem represents the vascular tissue (xylem) in the stem.
Conclusion :
Thus, the upward movement of water in plants is observed.
Precautions :
1. The Balsam plant taken should be fresh.
2. Handle the glass apparatus carefully.
3
EXPERIMENT NO 2
GIRDLING EXPERIMENT
Procedure:
1. Select a branch of a potted plant and gently remove its soft part from a small area of about
1cm, with the help of a razor blade.
2. Leave the branch after girdling for a week.
3. Observe what happens above and below the girdled area.
Observation:
The part of the stem above the girdle is swollen, while the lower part remains unchanged and
slowly starts drying.
Conclusion:
The food prepared by the leaves could not be carried below the girdled part of the stem, it
therefore collects above the girdle and that part of the stem becomes swollen.
GIRDLING EXPERIMENT
4
EXPERIMENT NO 3
OSMOSIS
Materials required: Two petri dishes, few raisins, grapes, sugar/ salt solution and water.
Procedure:
1. Take two petri dishes, take some raisins and keep them in a petri dish (labelled A),
containing water.
2. In another petri dish (labelled B) keep some grapes in sugar/ salt solution.
3. Observe the petri dishes after a few hours.
Observation:
1. In petri dish (A), endosmosis happens and raisins swell up.
2. In petri dish (B), exosmosis takes place and grapes become flaccid (shrink).
Conclusion:
Thus the process of osmosis is observed where the water molecules move from higher
concentration to lower concentration through a semi-permeable membrane.
Precautions:
1. Handle the glass apparatus carefully.
2. Do not keep the raisins soaked for a very long time, as fungus may start growing.
A. ENDOSMOSIS IN RAISINS
B. EXOSMOSIS IN RAISINS
5
EXPERIMENT NO 4
TRANSPIRATION IN PLANTS
Procedure:
1. Take a recently watered potted plant
3. Keep the pot in the sunlight for a few hours and then observe
Observation:
Drops of water found on the inner surface of the polythene sheet
Conclusion:
The presence of water inside the polythene bags confirms that plants give out extra amount of
water through stomata in the form of water vapour during transpiration
TRANSPIRATION IN PLANTS
6
EXPERIMENT NO 5
INTERNAL STRUCTURE OF HUMAN HEART
Description:
The heart is divided into a right and left side by the septum.
The heart has four chambers, two relatively small upper chambers called atria and two larger lower
chambers called ventricles. The walls of the ventricles are relatively thicker than atrial walls.
The two atria are separated from each other by a thin, muscular wall called the inter-atrial septum and
the right and left ventricles are by a thick-walled, inter-ventricular septum. The inter-atrial septum and
inter-ventricular septum prevent mixing of deoxygenated blood in the right side of the heart with
oxygenated blood in the left side of the heart.
The atria and ventricle of the same side are separated by a thick fibrous tissue called the atrioventricular
septum.
The opening between the right atrium and the right ventricle is guarded by a tricuspid valve, whereas a
bicuspid valve guards the opening between the left atrium and the left ventricle.
The largest artery is the aorta which arises from the left ventricle supplies blood to all the body parts
except lungs. Pulmonary artery that arises from the right ventricle carries deoxygenated blood to lungs.
The valves allow the blood to flow only in one direction, i.e. from the atria to the ventricles and from the
ventricles to the pulmonary artery or aorta.