CLASS 8 HOLIDAY HOMEWORK - BIOLOGy

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NATIONAL PUBLIC SCHOOL

YESHWANRTHPUR
BANGALORE

LABORATORY MANUAL
BIOLOGY
CLASS- 8

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LIST OF EXPERIMENTS

SERIAL NO NAME OF THE EXPERIMENT PAGE NUMBER

1 ASCENT OF SAP 3

2 GIRDLING EXPERIMENT 4

3 OSMOSIS 5

4 TRANSPIRATION 6

5 INTERNAL STRUCTURE OF HEART 7

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EXPERIMENT NO 1
ASCENT OF SAP

Aim : To observe upward movement of water in plants.

Materials required : Balsam plant, eosin, conical flask, water.

Procedure :
1. Take a cut shoot (a piece of stem with leaves) of balsam plant and dip it in diluted eosin in
a flask. Leave it for some time.
2. Take sections from different parts of the stem, make slides and examine the sections
under a microscope.

Observation :
1. Red colour is observed in the stem section.
2. The red coloured area of the stem represents the vascular tissue (xylem) in the stem.

Conclusion :
Thus, the upward movement of water in plants is observed.

Precautions :
1. The Balsam plant taken should be fresh.
2. Handle the glass apparatus carefully.

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EXPERIMENT NO 2
GIRDLING EXPERIMENT

Aim: To observe transport of food materials in plants.

Materials required: Potted plant, razor blade.

Procedure:
1. Select a branch of a potted plant and gently remove its soft part from a small area of about
1cm, with the help of a razor blade.
2. Leave the branch after girdling for a week.
3. Observe what happens above and below the girdled area.

Observation:
The part of the stem above the girdle is swollen, while the lower part remains unchanged and
slowly starts drying.

Conclusion:
The food prepared by the leaves could not be carried below the girdled part of the stem, it
therefore collects above the girdle and that part of the stem becomes swollen.

GIRDLING EXPERIMENT

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EXPERIMENT NO 3
OSMOSIS

Aim: To observe the process of osmosis.

Materials required: Two petri dishes, few raisins, grapes, sugar/ salt solution and water.

Procedure:
1. Take two petri dishes, take some raisins and keep them in a petri dish (labelled A),
containing water.
2. In another petri dish (labelled B) keep some grapes in sugar/ salt solution.
3. Observe the petri dishes after a few hours.

Observation:
1. In petri dish (A), endosmosis happens and raisins swell up.
2. In petri dish (B), exosmosis takes place and grapes become flaccid (shrink).

Conclusion:
Thus the process of osmosis is observed where the water molecules move from higher
concentration to lower concentration through a semi-permeable membrane.

Precautions:
1. Handle the glass apparatus carefully.
2. Do not keep the raisins soaked for a very long time, as fungus may start growing.

A. ENDOSMOSIS IN RAISINS

B. EXOSMOSIS IN RAISINS

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EXPERIMENT NO 4
TRANSPIRATION IN PLANTS

Aim : To demonstrate that water is given off during transpiration

Materials Required: Potted plant, polythene sheet

Procedure:
1. Take a recently watered potted plant

2. Enclose it in a transparent polythene bag

3. Keep the pot in the sunlight for a few hours and then observe

Observation:
Drops of water found on the inner surface of the polythene sheet

Conclusion:
The presence of water inside the polythene bags confirms that plants give out extra amount of
water through stomata in the form of water vapour during transpiration

TRANSPIRATION IN PLANTS

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EXPERIMENT NO 5
INTERNAL STRUCTURE OF HUMAN HEART

Aim: To study the internal structure of human heart.

Materials required: Model of Human Heart

Description:

 The heart is divided into a right and left side by the septum.
 The heart has four chambers, two relatively small upper chambers called atria and two larger lower
chambers called ventricles. The walls of the ventricles are relatively thicker than atrial walls.
 The two atria are separated from each other by a thin, muscular wall called the inter-atrial septum and
the right and left ventricles are by a thick-walled, inter-ventricular septum. The inter-atrial septum and
inter-ventricular septum prevent mixing of deoxygenated blood in the right side of the heart with
oxygenated blood in the left side of the heart.
 The atria and ventricle of the same side are separated by a thick fibrous tissue called the atrioventricular
septum.
 The opening between the right atrium and the right ventricle is guarded by a tricuspid valve, whereas a
bicuspid valve guards the opening between the left atrium and the left ventricle.
 The largest artery is the aorta which arises from the left ventricle supplies blood to all the body parts
except lungs. Pulmonary artery that arises from the right ventricle carries deoxygenated blood to lungs.
 The valves allow the blood to flow only in one direction, i.e. from the atria to the ventricles and from the
ventricles to the pulmonary artery or aorta.

INTERNAL STRUCTURE OF HEART


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