Celery Lab

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Celery Lab

By. Itthipat Chaicharoensukasem and Kiyo Steward

Inquiry paragraph:

In this lab, we measured transpiration, the process through which plants lose water,
primarily through leaves and stomata. Transpiration is vital for transporting water and
nutrients from roots to shoots. The experiment involved placing beakers into a heater to
observe the effect of temperature on transpiration rates. I hypothesize that transpiration
rates will increase in the warmer area. This expectation is based on the fact that
transpiration involves evaporation, and warmer temperatures generally enhance
evaporation. Therefore, I anticipate higher transpiration rates in the warmer area and lower
rates in the colder area. This aligns with the understanding that temperature influences the
efficiency of water loss through the leaves.

Design paragraph:

In this experiment, we examined the impact of temperature on celery transpiration, with the
independent variable being the temperature, the dependent variable being the transpiration
rate. To maintain fairness and accuracy, several controlled variables were upheld, including
consistent sizes of parafilm, fixed amounts of water and clay/tape, steady water
temperature, uniform environmental conditions around the celery, and a constant level of
humidity. These controlled variables were crucial to preventing skewed results and ensuring
that each beaker experienced similar conditions, thus contributing to the experiment's
reliability and fairness.

Procedure:

Materials: 6 celery stocks (about the same size), 6 beakers, 1 graduated cylinder, 1 pair of
scissors, 1 cuytting board, 1 ruler, 6 pieces of parafilm, 180ml of deionized water, and tape.

1. Use scissors to cut the celery stocks on the cutting board, and make sure they are the
same length.

2. Put each celery in a different beaker on its own.

3. Pour the deionized water into the graduated cylinder, to make sure the amount of water
is accurate.

4. After this, pour all of the water into the beaker with the celery, and repeat step 3 and 4,
until all the beakers are with 30 ml of water.

5. Use scissors, to cut a circular hole in the parafilm by folding it. Make sure the hole is big
enough for the celery stock.
6. Unfold the parafilm, peel the sticker off, and insert the celery stock into the hole, then put
it back into the beaker.

7. After this, make sure to tightly seal the parafilm onto the beaker, so not air gets in.

8. Fill the gaps between the celery and the parafilm, with tape, and clay.

9. Repeat steps 5-8 until all the beakers have parafilm on them, as airtight as possible.

10. Rip small pieces of tape, and write your initials, along with your groupmates. Also label
your beakers with tape as well.

11. After, you place your beakers to their labelled spot (hot, normal, cold).

12. Wait for 2 days, and observe and collect data.

13. Graph data once you have collected it, and finally, reflect on
your hypothesis, and your entire lab.

Evaluation:

In this experiment, our hypothesis about hotter temperatures increasing transpiration was
mostly accurate, as shown in the graph where celery in the heat had a much higher
transpiration rate. However, we faced challenges with small celery sticks, having to switch
beakers and restart some steps. Next time, we'll choose larger celery for a more
straightforward and accurate experiment. Despite the challenges, the lab was successful.
We cut the parafilm well, ensuring airtight seals, and kept our celery sticks evenly sized. Our
strong organization contributed to a well-structured data collection process. Overall, it was
a successful lab with areas for improvement in selecting larger celery.
Work site:

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