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Analytical Techniques Lab 3

NSU lab techniques (lab 3) for bio 1500.

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8 views3 pages

Analytical Techniques Lab 3

NSU lab techniques (lab 3) for bio 1500.

Uploaded by

loshara443
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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Alexis Balderas

16 February 2024

Sara Kaldas

BIOL 1500 Lab

Balderas 1

Table 1. Average % Error Data and SD (Class Data)


Average % error SD of error
P200 -88.82 4.89
P1000 -45.74 3.96

Figure 1. Average % error with


standard deviation
P200 and P1000

P1000

P200

-100.00 -80.00 -60.00 -40.00 -20.00 0.00


Percentage of error

Table 2. Absorbance Value Averages (Class Data)


Absorbance (A)
Concentration (mg/dL) Average SD
100 1.09 0.86
200 1.14 0.83
250 1.23 1.39
500 2.27 0.74
Unknown 1.65 1.32

Figure 2. Absorbance Value Averages (Class Data)


2.50
2.00
Absorbance (A)

f(x) = 0.225308333333333 x + 0.798908333333335


1.50
1.00
0.50
0.00
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
Concentration (mg/dL)
Alexis Balderas

16 February 2024

Sara Kaldas

BIOL 1500 Lab

Balderas 2

Discussion:

Data was collected by taking two different cuvettes. One called blank would be used to allow us

to zero out the spectrophotometer to get clear results when measuring the maximal absorbance

for the second cuvette labeled as number 5. First, we inserted cuvette #1 into the

spectrophotometer which allowed us to ensure that the spectrophotometer was reading properly,

after getting the reading for cuvette #1 and zeroing it out, we placed cuvette #5 and got our

readings. We would repeat this process every time we would increase wavelength (nm) by 25.

Once the data was collected, we placed the data in a table and calculated the averages. Looking

over the averages you would see that 425nm had the highest absorbance compared to all the

other tested wavelengths.

Lab Homework:

1. I learned that accuracy and precision are not the same thing. Our results for procedure 1

are very precise because the results aren’t the same but they’re close together meaning

that our results are precise, we had some issues with accuracy since there were some

measures/results that were way off compared to other results we obtained.

2. -82.300% error for p200 -46.940% error for p1000

a. What may have caused the percent error would be us not measuring out the right

amount of water we needed to weigh.


Alexis Balderas

16 February 2024

Sara Kaldas

BIOL 1500 Lab

Balderas 3

3. A fasting patient wouldn’t meet the criteria for diabetes mellitus. My diagnosis would be

that they have very low sugar meaning that they’re hypoglycemic

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