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    Table & Graph

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    Athirah Kamil
    The document contains three tables that report the distance traveled by DNA bands of different sizes from the wells of three separate gels: an Ultra Ranger 1Kb DNA ladder, Gel A, and Gel B. The distance traveled decreases linearly with the logarithm of the fragment size for all three gels, as shown by the plots and linear regression analyses, indicating that smaller DNA fragments migrate farther than larger ones in agarose gel electrophoresis.

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    Attribution Non-Commercial (BY-NC)
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    Table & Graph

    Uploaded by

    Athirah Kamil
    91 views5 pages
    The document contains three tables that report the distance traveled by DNA bands of different sizes from the wells of three separate gels: an Ultra Ranger 1Kb DNA ladder, Gel A, and Gel B. The distance traveled decreases linearly with the logarithm of the fragment size for all three gels, as shown by the plots and linear regression analyses, indicating that smaller DNA fragments migrate farther than larger ones in agarose gel electrophoresis.

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    © Attribution Non-Commercial (BY-NC)

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    The document contains three tables that report the distance traveled by DNA bands of different sizes from the wells of three separate gels: an Ultra Ranger 1Kb DNA ladder, Gel A, and Gel B. The distance traveled decreases linearly with the logarithm of the fragment size for all three gels, as shown by the plots and linear regression analyses, indicating that smaller DNA fragments migrate farther than larger ones in agarose gel electrophoresis.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    91 views5 pages

    Table & Graph

    Uploaded by

    Athirah Kamil
    The document contains three tables that report the distance traveled by DNA bands of different sizes from the wells of three separate gels: an Ultra Ranger 1Kb DNA ladder, Gel A, and Gel B. The distance traveled decreases linearly with the logarithm of the fragment size for all three gels, as shown by the plots and linear regression analyses, indicating that smaller DNA fragments migrate farther than larger ones in agarose gel electrophoresis.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 5

    BAND SIZE (bp)

    LOG SIZE
    DISTANCE (cm)
    1
    24000
    4.3802
    0.20
    2
    14000
    4.1461
    0.30
    3
    10000
    4.0000
    0.50
    4
    8000
    3.9030
    0.70
    5
    6000
    3.7781
    0.90
    6
    5000
    3.6990
    1.15
    7
    4000
    3.6021
    1.45
    8
    3000
    3.4771
    1.90
    9
    2500
    3.3979
    2.40
    10
    2000
    3.3010
    2.85
    11
    1500
    3.1761
    3.60
    12
    1000
    3.0000
    4.65
    13
    700
    2.8451
    5.65
    14
    500
    2.6990
    6.35
    15
    300
    2.4771
    7.40
    Table 1. Distance travelled by each of the DNA bands of Ultra Ranger 1Kb DNA ladder from wells.

    LAMBDA
    DISTANCE (cm)
    LOG SIZE
    SIZE (bp)
    FRAGMENT
    1 (biggest)
    1.6
    3.6994
    2
    3
    3.383
    3
    4.5
    3.044
    4
    5
    2.931
    5
    5.4
    2.8406
    6
    7.1
    2.4564
    7
    7.4
    2.3886
    Table 2. Distance travelled by each of the DNA bands of Gel A from wells.

    LAMBDA
    DISTANCE (cm)
    LOG SIZE
    SIZE (bp)
    FRAGMENT
    1 (biggest)
    1.6
    3.6994
    2
    3.1
    3.3604
    3
    4.3
    3.0892
    4
    4.65
    3.0101
    5
    5
    2.931
    6
    6.1
    2.6824
    7
    6.9
    2.5016
    Table 3. Distance travelled by each of the DNA bands of Gel B from wells.

    5004.953
    2415.461
    1106.624
    853.1001
    692.7874
    286.0224
    244.6809

    5004.953
    2292.979
    1228.005
    1023.529
    853.1001
    481.2824
    317.3949

    Distance travelled by each of the DNA bands of Ultra Ranger 1Kb DNA ladder from wells (cm)
    Vs. Fragment length (log bp)
    5
    4.5

    Fragment length (log bp)

    4
    3.5
    3
    y = -0.2261x + 4.0617
    R = 0.9369

    2.5
    2

    log bp
    Linear (log bp)

    1.5
    1
    0.5
    0
    0

    Distance from well (cm)

    bp
    distance
    24000
    0.2
    14000
    0.3
    10000
    0.5
    8000
    0.7
    6000
    0.9
    5000
    1.15
    4000
    1.45
    3000
    1.9
    2500
    2.4
    2000
    2.85
    1500
    3.6
    1000
    4.65
    700
    5.65
    500
    6.35
    300
    7.4

    log size
    4.380211
    4.146128
    4
    3.90309
    3.778151
    3.69897
    3.60206
    3.477121
    3.39794
    3.30103
    3.176091
    3
    2.845098
    2.69897
    2.477121

    Distance travelled by each of the DNA bands of Gel A from wells (cm) Vs. Fragment length (log bp)

    Fragment length (log bp)

    3.5
    3
    2.5

    y = -0.226x + 4.061
    R = 1

    Series1

    1.5

    Linear (Series1)

    1
    0.5
    0
    0

    Distance from well (cm)

    bp
    antilog
    distance log size
    5004.953 -0.3616
    1.6
    3.6994
    2415.461
    -0.678
    3
    3.383
    1106.624
    -1.017
    4.5
    3.044
    853.1001
    -1.13
    5
    2.931
    692.7874 -1.2204
    5.4
    2.8406
    286.0224 -1.6046
    7.1
    2.4564
    244.6809 -1.6724
    7.4
    2.3886

    Distance travelled by each of the DNA bands of Gel B from wells (cm) Vs. Fragment length (log bp)

    4
    3.5

    Fragment length (log bp)

    3
    y = -0.226x + 4.061
    R = 1

    2.5

    log bp

    Linear (log bp)


    1.5

    Linear (log bp)

    1
    0.5
    0
    0

    Distance from well (cm)

    bp
    antilog
    distance log size
    5004.953 -0.3616
    1.6
    3.6994
    2292.979 -0.7006
    3.1
    3.3604
    1228.005 -0.9718
    4.3
    3.0892
    1023.529 -1.0509
    4.65
    3.0101
    853.1001
    -1.13
    5
    2.931
    481.2824 -1.3786
    6.1
    2.6824
    317.3949 -1.5594
    6.9
    2.5016

    5
    4.5

    Fragment length (log bp)

    4
    3.5
    3
    y = -0.2261x + 4.0617
    R = 0.9369

    2.5
    2

    log bp
    Linear (log bp)

    1.5
    1
    0.5
    0
    0

    Distance from well (cm)

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