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Table & Graph

The document contains three tables that report the distance traveled by DNA bands of different sizes from the wells of three separate gels: an Ultra Ranger 1Kb DNA ladder, Gel A, and Gel B. The distance traveled decreases linearly with the logarithm of the fragment size for all three gels, as shown by the plots and linear regression analyses, indicating that smaller DNA fragments migrate farther than larger ones in agarose gel electrophoresis.

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Athirah Kamil
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© Attribution Non-Commercial (BY-NC)
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Download as DOCX, PDF, TXT or read online on Scribd
102 views

Table & Graph

The document contains three tables that report the distance traveled by DNA bands of different sizes from the wells of three separate gels: an Ultra Ranger 1Kb DNA ladder, Gel A, and Gel B. The distance traveled decreases linearly with the logarithm of the fragment size for all three gels, as shown by the plots and linear regression analyses, indicating that smaller DNA fragments migrate farther than larger ones in agarose gel electrophoresis.

Uploaded by

Athirah Kamil
Copyright
© Attribution Non-Commercial (BY-NC)
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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BAND SIZE (bp)

LOG SIZE
DISTANCE (cm)
1
24000
4.3802
0.20
2
14000
4.1461
0.30
3
10000
4.0000
0.50
4
8000
3.9030
0.70
5
6000
3.7781
0.90
6
5000
3.6990
1.15
7
4000
3.6021
1.45
8
3000
3.4771
1.90
9
2500
3.3979
2.40
10
2000
3.3010
2.85
11
1500
3.1761
3.60
12
1000
3.0000
4.65
13
700
2.8451
5.65
14
500
2.6990
6.35
15
300
2.4771
7.40
Table 1. Distance travelled by each of the DNA bands of Ultra Ranger 1Kb DNA ladder from wells.

LAMBDA
DISTANCE (cm)
LOG SIZE
SIZE (bp)
FRAGMENT
1 (biggest)
1.6
3.6994
2
3
3.383
3
4.5
3.044
4
5
2.931
5
5.4
2.8406
6
7.1
2.4564
7
7.4
2.3886
Table 2. Distance travelled by each of the DNA bands of Gel A from wells.

LAMBDA
DISTANCE (cm)
LOG SIZE
SIZE (bp)
FRAGMENT
1 (biggest)
1.6
3.6994
2
3.1
3.3604
3
4.3
3.0892
4
4.65
3.0101
5
5
2.931
6
6.1
2.6824
7
6.9
2.5016
Table 3. Distance travelled by each of the DNA bands of Gel B from wells.

5004.953
2415.461
1106.624
853.1001
692.7874
286.0224
244.6809

5004.953
2292.979
1228.005
1023.529
853.1001
481.2824
317.3949

Distance travelled by each of the DNA bands of Ultra Ranger 1Kb DNA ladder from wells (cm)
Vs. Fragment length (log bp)
5
4.5

Fragment length (log bp)

4
3.5
3
y = -0.2261x + 4.0617
R = 0.9369

2.5
2

log bp
Linear (log bp)

1.5
1
0.5
0
0

Distance from well (cm)

bp
distance
24000
0.2
14000
0.3
10000
0.5
8000
0.7
6000
0.9
5000
1.15
4000
1.45
3000
1.9
2500
2.4
2000
2.85
1500
3.6
1000
4.65
700
5.65
500
6.35
300
7.4

log size
4.380211
4.146128
4
3.90309
3.778151
3.69897
3.60206
3.477121
3.39794
3.30103
3.176091
3
2.845098
2.69897
2.477121

Distance travelled by each of the DNA bands of Gel A from wells (cm) Vs. Fragment length (log bp)

Fragment length (log bp)

3.5
3
2.5

y = -0.226x + 4.061
R = 1

Series1

1.5

Linear (Series1)

1
0.5
0
0

Distance from well (cm)

bp
antilog
distance log size
5004.953 -0.3616
1.6
3.6994
2415.461
-0.678
3
3.383
1106.624
-1.017
4.5
3.044
853.1001
-1.13
5
2.931
692.7874 -1.2204
5.4
2.8406
286.0224 -1.6046
7.1
2.4564
244.6809 -1.6724
7.4
2.3886

Distance travelled by each of the DNA bands of Gel B from wells (cm) Vs. Fragment length (log bp)

4
3.5

Fragment length (log bp)

3
y = -0.226x + 4.061
R = 1

2.5

log bp

Linear (log bp)


1.5

Linear (log bp)

1
0.5
0
0

Distance from well (cm)

bp
antilog
distance log size
5004.953 -0.3616
1.6
3.6994
2292.979 -0.7006
3.1
3.3604
1228.005 -0.9718
4.3
3.0892
1023.529 -1.0509
4.65
3.0101
853.1001
-1.13
5
2.931
481.2824 -1.3786
6.1
2.6824
317.3949 -1.5594
6.9
2.5016

5
4.5

Fragment length (log bp)

4
3.5
3
y = -0.2261x + 4.0617
R = 0.9369

2.5
2

log bp
Linear (log bp)

1.5
1
0.5
0
0

Distance from well (cm)

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