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Procedia Food Science 3 (2015) 1 – 18

The First International Symposium on Food and Agro-biodiversity (ISFA2014)

The Utilization of Extract Durian (Durio zibethinus L.) Seed Gum

as an Emulsifier in Vegan Mayonnaise
Melanie Corneliaa* , Titri Siratantria , Retna Prawitaa
a
Food Technology, Universitas Pelita Harapan, Karawaci- Tangerang 15811 , Indonesia

Abstract

Durian is the most popular seasonal fruit in Indonesia. Only 1/3 of durian is edible, whereas the
seeds are thrown away. This waste is potential of value-added such as seed gum. Mayonnaise is o/w
emulsion based product use egg yolk as an emulsifier, but in this research, durian seed gum (2,5%,
3%, 3,5%, 4%, and 4,5%) has been used to replace on egg yolk in making vegan mayonnaise. The
best formulation of vegan mayonnaise from 4% durian seed gum was compared to commercial and
the control as well. This result is as good as mayonnaise with egg yolk, and the commercial
mayonnaise.

©2015
© 2014 TheThe Authors.
Authors. PublishedPublished by This
by Elsevier Ltd. Elsevier Ltd.
is an open access article under the CC BY-NC-ND license
Peer-review under responsibility of the organizing committee of Indonesian Food
(http://creativecommons.org/licenses/by-nc-nd/4.0/). Technologist
Community
Peer-review under responsibility of the organizing committee of Indonesian Food Technologist Community

Keywords: durian seed gum, protein, oil in water emulsion, vegan mayonnaise.

INTRODUCTION

Durian is a seasonal fruit that most popular in Southeast Asia, particularly Malaysia,
Indonesia, Thailand, and Philippines [16]. Central Bureau of Statistics of the Republic of Indonesia
states that the average durian production increased from 17,405 tons in 1999 to 741,831 tons in
2003, and by the end of 2011 to 883,969 tons. Only a third of the durian fruit are edible, while the
seed (20-25%) and the skin is usually discarded. Utilization of durian seed is still limited.
According to research [2], durian seed can be used as a thickener because of the content of
hydrocolloid (water-solube gum).
* Corresponding author
E-mail address: [email protected]

2211-601X © 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/).
Peer-review under responsibility of the organizing committee of Indonesian Food Technologist Community
doi:10.1016/j.profoo.2015.01.001
2 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18

Previous studies revealed that the natural polymer of gum durian seed had emulsifying
activity oil in water (o/w). Natural gum was a polysaccharide consisting of monosaccharides linked
through glycoside bonds [18]. Natural gum polymers are usually complex, generated a lot of plants,
such as carrageenan, pectin, CMC, starch, guar gum, and sodium carboxyl. Methyl cellulose was
used as a gelling agent, thickener, stabilizer, emulsifier [16]. Liquid extraction was one of the most
common techniques used for the extraction of gum [2]. Hydrocolloid can be used as a natural
emulsifier and thickener in products such as jam, jelly, and mayonnaise. Mayonnaise, an emulsion
of oil in water, is generally using egg yolks as the emulsifying agents [7]. However, the constraints
are the risk of contamination with Salmonella sp. on raw chicken eggs, the price and quality of
chicken eggs, as well as the high content of cholesterol in egg yolk [19]. For some vegetarians can
not consume egg-based foods, lead the research on protein utilization as an alternative emulsifying
agents developed in recent years [20]. Gum durian seeds can be used as an alternative emulsifying
oil in water (o/w) on a more stable mayonnaise expected quality, during storage and produce
characteristics similar to mayonnaise in general. This research durian used gum durian seed as egg
yolk replacer to make vegan mayonnaise
MATERIALS AND METHODS
These were materials for this research: gum durian seed was extracted from Indonesia local
durian, 96% food grade ethanol, soybean oil include mayonnaise, vinegar ( 25% acetic acid) and
vegan mayonnaise commercial for comparison.
Methods of experimental were divided into two stages, preliminary and the main research.
Preliminary research were conducted to produce extract gum from durian seed. Extraction of gum
durian seed can be seen as Fig. 1.
The main research was to determine the best formulation on making mayonnaise using durian
seed gum as an emulsifier, then compared it with commercial mayonnaise and mayonnaise
vegetarian as a control. Experimental design factor for main research were ratio of water /oil (A0)
is 30% : 70% and gum concentration.: 2,5% (B0), 3% (B1), 3,5% (B2), 4% (B3), and 4,5% (B4)
Mayonnaise was made in 5 (five) formulations with different concentrations of emulsifiers
(gum durian seed) were used. Formulations for making mayonnaise can be seen in Table 2.
 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18 3

Durian Seed
↓
Washing
↓
Peeling
↓
Cutting
↓
Drying in cabinet dryer for 24-48 hrs (50°C)
↓
Mixing
↓
Durian Seed Flour (shieving 80 mesh)
↓
Durian Seed Flour are extracted by using demineralized water
( w/s ratio = 35,5 : 1 w/w ; pH 11,9 ; Temp 85oC) for 1 hour
↓
Extract (Slurry) were centrifuged for 10 mins, 1200 rpm

Supernatant Sediment
↓
Mixed with 96% ethanol (food grade) 2 times supernatant volume
↓
gum extract , drying for 12 hours
↓
blending and shieving 120 mesh
↓
Gum Extract Durian Seed Flour

Fig 1. Flow Diagram of Gum Extract Durian Seed

Source: Mirhosseini and Tabatabaee [16].

Tabel 1. Experimental Design of Main Research

water : oil (A0)
gum (B)
30% : 70%
2,5% (B0) (A0B1)1 (AoB0)2 (A0B0)3
3% (B1) (A0B2)1 (A0B1)2 (A0B1)3
3,5% (B2) (A0B3)1 (A0B2)2 (A0B2)3
4% (B3) (A0B4)1 (A0B3)2 (A0B3)3
4,5% (B4) (A0B5)1 (A0B4)2 (A0B4)3

Tabel 2. Formulation Mayonnaise with some level of substitution Gum

Formulation components
I II III IV V
Vegetable oil, (g) 70 70 70 70 70
Emulsifier (gum), (g) 2.5 3 3.5 4 4.5
Vinegar, (g) 3.5 3.5 3.5 4.5 4.5
Sugar, (g) 2.75 2.75 2.75 2.75 2.75
Salt, (g) 1.5 1.5 1.5 1.5 1.5
Pepper powder, (g) 0.5 0.5 0.5 0.5 0.5
Water, (g) 30 30 30 30 30
4 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18

Flowchart of making vegan mayonnaise could be seen in Figure 2.

Gum with a certain concentration dissolved into 20 ml of water

↓
mixed with vinegar solution to achieve a pH 3 to 4
↓
Gum solution, 70 ml oil, 10 ml residual water were poured into the container
↓
mixed with high speed for 30 seconds
↓
Salt, sugar, pepper are incorporated into the dough has formed
↓
The dough is then stirred with a baloon whisk for 15 seconds
↓
Vegan Mayonnaise

Fig. 2 Flowchart Making Mayonnaise

Source: Modified Goush et al. [11]

RESULTS AND DISCUSSION

Yield of seed from local (Indonesian) durian was 31.08%, while the imported durian seed
from Thailand (Monthong) is 4.6%. So, the research is used local durian for extracting the gum
based on the avove yield. Durian seed from local durian are identified in Bogor Botany Research
Center for Biology, and the results has the Latin name Durio zibethinus L., with ordo Malvaceae.
Extracted gum durian seeds were analyzed and the result of physico-chemical characteristics
were as per below.

Tabel 3. Result of physico-chemical analysis gum from durian seed

Gum Characteristics
Result
Durian Seed
Yield (%) 58.80 ± 7.32
Colour (0Hue) 50.39 ± 6.17
Water absorption capacity(%) 1112.15 ± 158.31
Fat absorption capacity (%) 396.78 ± 4.67
Total amino acid (mg/100g) 2223.18 ± 96.03
Density “Kamba” (g/ml) 0.53 ± 0.12

Gum yield from extracting durian seed was 58.80%. According to [16] gum durian seed
yield is around 34.2-72.8% with an average of 56.40%. According to [22], extraction time and
extraction temperature will affect the gum yield. In this research, colour of the gum is brown and
has the chromameter result 50,39 0Hue due to natural pigment, tannin and husk of durian seed.
Below was the result of amino acid analysis from durian seed gum.
 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18 5

Tabel 4. Compositon of Amino Acid Durian Seed Gum

Amino Acid Quantity (mg/100mg Gum durian seed)

Essential valine 141.08
Amino Acid methionine 31.19
lysine 199.37
isoleucine 100.76
leucine1 59.73
phenylalanine 69.95
threonine 99.90
cysteine 0
tyrosine 38.97
Total 840.95
Non Essential aspartic acid 288.62
Amino Acid serine 150.53
glutamic acid 379.91
glycine1 23.65
alanine 172.33
proline 154.22
Total 1268.86
Semi Essential arginine 70.99
Amino Acid histidine 42.38
Total 113.37
Total Amino Acid 2223.18

Based on the analysis result of amino acids durian seed gum (Table 4), most of the
constituent amino acids has polar R groups, so it making easy to absorb water. The durian seed gum
has the ability to absorb more water than fat, so it can help the dispersion of oil in water emulsion
which led to the formation of oil in water (o/w) [9]. It can be seen that the durian seed gum has a
hydrophobic amino acid (non-polar) less than the hydrophilic (polar). [21] revealed that the higher
hidrophobicity, the stronger the protein adsorbed at the interface, so it has the greater ability to
form and stabilize emulsions.

Emulsion Activity and Stability

Emulsion activity and emulsion stability of durian seed gum were tested at the diffrent pH
level, at pH 3, pH 4, pH 5, pH 6, pH 7.5, and pH 9 . The result could be seen as follows:
6 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18

Fig 3. Graph of emulsion Activity of durian seed gum

Fig 4. Graph of emulsion stability of durian seed gum

Based on the above data, it can be estimated isoelectric point of durian seed gum is in the
range of pH 4.5. [4] and [15] stated that the activity of the protein solution emulsion will generally
be at the minimum point at pH isoelectric. The ability of the emulsion activity decreases with
increasing pH, so at pH 3 emulsions activity is at its highest point.
Emulsion stability at pH 3 to 4.5 is relatively unstable, moreover this is zero because there is
no emulsion is formed, but rather a process that occurs is creaming, whereas at pH 6-9 is relatively
high emulsion stability and reaches a maximum at pH 7 , 5-9. No effect of pH on emulsion stability
durian seed gum. Based on these patterns, the durian seed gum is thought to have an isoelectric
point in the range of pH 4.5 as at pH activity and stability of the emulsion is the lowest. [15]
revealed that the isoelectric point, electrostatic repulsion refuse will reach a minimum value, so that
 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18 7

the stability of the emulsion also reached a minimum. This is due to a decrease in protein solubility
and decrease the amount of the total charge on the protein surface.

Scoring test
Scoring mayonnaise organoleptic testing were done using the scoring method 7 scale by 70
trained and semi-trained panelists. The parameters assessed were sour flavor, sour taste, color, after
taste, and texture. The results can be seen in the graphs below.

Fig 5. S Effect of Gum Concentration on the Sour Flavor in Vegan Mayonnaise

Note: - the same superscript in the same column means there is no significant difference at α = 0.05
1: strongly not sour, 7: very flavorful acid

Based on the statistical results for the parameters aroma shows that there is no significant
difference (P> 0.05) between the concentration of gum durian seed flour is added to the scoring
results produced mayonnaise scent. Figure 5 show that scores on the mayonnaise with the sour scent
level of the different gum concentrations ranging from 4-5. It can be seen that the mayonnaise with
2.5% gum, 3% gum concentration levels, 3.5%, 4% and 4.5% did not differ significantly. So,
overall it can be concluded that there is no effect of additional gum concentration on the aroma of
durian seed gum acid. This is because, the scent is more dominant acid in mayonnaise and sour
aroma influenced by the amount of acetic acid were added.
8 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18

Fig 6. Effect of Gum Concentration on The Sour Taste in Vegan Mayonnais

From Figure 6, it can be seen that the higher the concentration of the gum added, the lower
assessment sour taste in mayonnaise. This proves that sour taste is perceived by panelists covered
by the typical flavor of the gum itself. Overall it can be seen that there is the effect of adding gum to
the sour taste of durian seed mayonnaise.

Fig 7. Effect of Gum Concentration on The Color in Vegan Mayonnaise

 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18 9

Fig 8. Effect of Gum Concentration on the Texture of the Vegan Mayonnaise

Scoring the test results for the color parameters based on the statistics show that there is a
significant difference (P <0.05) between the concentration of gum durian seed flour is added to the
scoring results of the color produced mayonnaise.

Fig 9. Effect of Gum Concentration on the Aftertaste of Vegan Mayonnaise

It can be seen that the higher the concentration of added gum, the assessment has result the
higher the gritty texture of mayonnaise. Adding gum at a concentration of 2.5% significantly
different with 4.5% concentration, while the concentration did not differ significantly by 3%, 3.5%
and 4%. This is due to the addition of high gum, causing more soluble gum, and ultimately affect
the texture, resulting in the sandy texture of mayonnaise with 4.5% concentration of highly rated.
The test results for parameters aftertaste based scoring statistics show that there was no
significant difference (P> 0.05) between the concentration of gum durian seed flour is added to the
10 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18

scoring results produced mayonnaise aftertaste. Based on the results, did not reveal any difference
in the final flavor in the mayonnaise on the fifth formulation. It can be concluded that the more gum
are added does not affect the final flavor in the mayonnaise
The overall acceptance results of the flavor, color, texture, and aftertaste on the fifth
formulation is acceptable by the panelists.
Best Formulation of Vegan Mayonnaisse

Fig 10. Mayonnaise durian seed gum

From the overall results of physicochemical analysis and the scoring test, there is no
significant difference among all the formulations, whereas in test scoring mayonnaise with 4% gum
concentration levels tend to be better than the total overall parameters of texture, color, flavor,
aroma, and aftertaste. Therefore, the selection of the best mayonnaise is mayonnaise with gum
concentration of 4%. Determination of the best formulation is reinforced by [14] which states that
the mayonnaise and salad dressings generally use eggs as an emulsifier must not be less than 4%.
Therefore, this research choose 4% gum concentration as the best concentration added, apart from
the assessment test and acceptance test which is acceptable by the panelists, as well as to meet the
minimum standards.

Pair Comparison Test

Pair comparison test is conducted to determine whether there is any difference between the best
mayonnaise earlier stage research (vegan mayonnaise with addition 4% gum durian seed) with
commercial mayonnaise and estimate the intensity difference
 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18 11

Figure 11 Comparison Value Pairs Mayonnaise on the rate of 4% Gum Concentration

Remarks: + better 0 no different; - More is not better

Based on the average value of the comparison pairs mayonnaise with a concentration of 4%
(Figure 11), it can be seen that, mayonnaise with a concentration of 4% is considered to have better
flavor than commercial mayonnaise. In terms of color, the color of vegan mayonnaise produced is
not as white as commercial mayonnaise, but mayonnaise color gum assessed as good with color
than commercial mayonnaise. In terms of taste, flavor gum mayonnaise is not better than
commercial mayonnaise, this is because the gum itself has a distinctive taste, and ingredients used
to flavor enhancer derived only from the vinegar, salt, and sugar, while the commercial mayonnaise
using milk, which can give a better taste. Viscosity of vegan gum mayonnaise considered better
than commercial mayonnaise. Viscosity is highly correlated with success in forming and stabilizing
protein emulsion systems. Overall vegan mayonnaise with gum were not considered to have
different characteristics with commercial mayonnaise.. From these results, it is proved that the
durian seed gum can be used as an alternative emulsifiers in oil-in water emulsion system as in
mayonnaise products.

Best formulation of vegan mayonnaise analysis

The results of the vegan mayonnaise with the best gum added formulation are analyzed for
physical-chemical characteristics which include viscosity, emulsion stability, pH value, fat globula
12 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18

size, proximate analysis, and also color.

Viscosity
The results of viscosity measurements with the addition of mayonnaise durian seed gum as
an emulsifier is at 3160 cps, while the viscosity of commercial mayonnaise 3346,67cps [1].

Emulsion Stability and Globula Fat

Based on this research, the results of emulsion stability of vegan mayonnaise gum is
80.34%, this is because of globula fat produced is still relatively large. Figure globula fat produced
can be seen in Figure 12. According to [12], factors affecting emulsion stability of mayonnaise is
the type and amount of added emulsifiers, fat globula size, viscosity, method of stirring or agitation
and temperature. Therefore, the emulsion stability of mayonnaise gum is still not perfect. The
results of the size of the fat in the mayonnaise globula gum is 36.4 lm and can be seen as follow:

pH value
Results vegan gum mayonnaise emulsion activity is at its highest at pH 3, and the lowest at
pH 4.5, therefore, the resultingvegan gum mayonnaise has a pH of 3.97, the pH is closer to pH 4.5.

Tabel 5. Proximate Analysis of Vegan Mayonnaise Gum Durian seed

Analysis Vegan Mayonnaise Gum Indonesian Standard (using
Durian seed (4% gum) eggyolk)
Water Content (%) 32.38 ± 0.34 max.30
Ash (%) 0.72 ± 0.01
Fat (%) 60.36 ± 1.26 min. 65
Protein (%) 0.16 ± 0.03 min 0.9
Carbohydrate (%) 6.54 ± 0.94 max. 4

From the results of the proximate analysis above, vegan mayonnaise durian seeds with gum
emulsifiers have a higher water content than the Indonesian Standard for mayonnaise. It is because
when gum are extracted requires water as an emulsion.
 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18 13

Figure 12. Sightings globula Durian Seed Gum Fat Mayonnaise with 40x Magnification Microscope

Protein on egg mayonnaise tends to be higher, because the protein content of chicken eggs is
high. This is made clear by [12] which revealed that the chicken egg yolk contains complex
compounds that are essential in the formation of an emulsion, lecithin phospholipids are compounds
and some compounds such as lipoprotein lipovitellin, lipovitellinin, and livetin. Sometimes protein
content of commercial mayonnaise tends to be lower, because it does not use eggs as emulsifiers.

Comparison of Best Formulation Vegan Gum Durian Seed with Mayonnaise Commercial
Vegetarian and Egg Mayonnaise as a Control

The results of the comparison analysis of three mayonnaise characteristics can be seen in
Table 6. Commercial mayonnaise moisture content has a higher than vegan mayonnaise gum and
control, this is because the raw material of commercial mayonnaise are added with milk and water.
Mayonnaise control has a water content lowest, due to during the process are not added water to
the formulation. All water content results are in accordance with Indonesian standards for egg
mayonnaise, where the water content of vegan mayonnaise durian seed gum is 32.38%. approached
the standard.
14 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18

Table 6. Results Comparison of Best Formulation Vegan Gum Durian Seed with Mayonnaise Commercial Vegetarian
and Egg Mayonnaise as a Control

Vegan Mayonnaise Indonesia

Mayonnaise
Mayonnaise egg (as a Standard for Egg
Analysis Vegetarian
Gum Durian control) Mayonnaise
Commercial
Seed (4% gum) (0% gum)

Water content (%) 32.38 59.5 8.6 max.30

Fat Content (%) 60.36 20 52.65 min. 65
Protein (%) 0.16 0.5 2.5 min 0.9
Fat Globula Size (µm) 36.4 1.75 2.5
Emulsion Stabilization (%) 80.34 100 83.65
pH 3.97 3.40 4.67
Viscosity (cps) 3160 3346 4560
Color (0Hue) 72.97 71.35 80.61

Between the three contents of fat mayonnaise, commercial mayonnaise has the lowest fat
content, this is because the water content of commercial mayonnaise is higher than.. Of the three
mayonnaise, fat content below the established standard.
Protein on egg mayonnaise are tends to be higher, because the protein content of chicken
eggs is high. This is made clear by [12] which revealed that the chicken egg yolk contains complex
compounds that are essential in the formation of an emulsion, lecithin phospholipids are compounds
and some compounds such as lipoprotein lipovitellin, lipovitellinin, and livetin. Commercial
mayonnaise protein levels tend to be lower, because it does not use eggs as emulsifiers.
pH measurements of mayonnaise is another important parameter in the mayonnaise
emulsion system. Based on the results, it can be seen in the table above that pH of vegan
mayonnaise gum around 3.97, not much different from the pH of commercial mayonnaise (3.40);
While pH of egg mayonnaise control has a pH value of about 4.67. Gum durian seeds have
isoelectric pH of about 4.5, while the eggyolk has an isoelectric pH of about 5.3. It will affect the
pH mayonnaise. According to [10], the pH of normal mayonnaise is 3.70. Based on [10], vegan
mayonnaise gum has the same pH with a pH of normal mayonnaise.
Other parameters could be seen in Figure 13 which shows that egg mayonnaise (b) has the
most fat globula small size (about 6.2 µm), followed by (c) commercial mayonnaise (about 9.2 µm)
and vegan mayonnaise gum (around 36.4 µm). This was because, commercial mayonnaise
homogenization process using a high-speed homogenizer, so that the size of the resulting fat
globula smaller than, while the homogeneous compared with vegan gum mayonnaise are done by
stick mixer. Globula fat of eggs mayonnaise (control) have a greater than commercial, this was
because the process of making mayonnaise using a whisk manually by hand, so it is not as good as
a homogenizer.
Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18 15

(a)

(b)
16 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18

(c)

Figure 13. Sightings fat globula of Mayonnaise with 1000x magnification. (a) Vegan Mayonnaise
with Durian Seed Gum; (b) Mayonnaise with controls (0% gum), (c) Commercial Mayonnaise

The next parameter was the stability of the emulsion. From the statistical results of the
emulsion stability was found that no significant difference (α <0.05) between three mayonnaise, but
the emulsion stability of mayonnaise durian seed gum tends to be lower. This was consistent with
the results of the research [11] which also proves that the mayonnaise that did not use egg yolks as
an emulsifier (100% using the gum-protein) is more unstable than the mayonnaise was still using
chicken egg yolk by 75%, 50%, and 25% of the total emulsifying agents were used. Meanwhile,
vegetarian mayonnaise has a most excellent emulsion stability, this was because commercial
mayonnaise homogenization process uses a high-speed homogenizer, so that the size of the
resulting fat globula smaller than and homogeneous compared with gum mayonnaise made with
stick mixer. For viscosity analysis, from the statistical results of viscosity which was obtained, there
is no significant differences (α <0.05) between three mayonnaise. It can be seen that the overall
results vegan mayonnaise gum shows viscosity lower than the other viscosity values, but if compare
the results to vegetarian mayonnaise commercial with nearly the same, the amount of 3160cps and
3346,67cps. From the statistical results of the color that was obtained, there was no significant
differences (α <0.05) between three mayonnaise, it could be seen that all have three degrees of hue
 Melanie Cornelia et al. / Procedia Food Science 3 (2015) 1 – 18 17

values were not big different. Which was the result of vegan gum mayonnaise 72.97, commercial
mayonnaise at 71.35, and 80.61 for an egg mayonnaise. The results of the three mayonnaise was
still quite the same range, which according to [13], the result was still quite 0Hue of 54-90 yellow
red.

CONCLUSION
Gum from local (Indonesia) durian seeds have yield about 58%. Based on the analysis, gum
has the good capability of water and fats absorption, as well as emulsion activity and emulsion
stability were high and fairly absorption stable. Gum durian seeds have low activity and emulsion
stability at pH 4.5 and the highest at pH 3. Gum durian seeds have hydrophobic amino acid (non-
polar) 829.26 mg / g of protein and hydrophilic amino acids (polar) 1393.92 mg / g of protein. The
durian seed gum could be used as an emulsifier for making mayonnaise vegan (no eggs), because it
produced fairly stable emulsion, good texture, and fat globula size was not too large. Based on the
test results and scoring, it was selected the best vegan mayonnaise were with addition of 4% gum
concentration, and have the aroma of sour, also sour taste intensity, and brown color. Pair
comparison test results showed that the mayonnaise with durian seed gum was not considered to
have different characteristics with the commercial mayonnaise. Thus, the seeds of durian were not
just a waste, but could be used as emulsifiers in making of vegan mayonnaise.

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Presented at ISFA (September 16-17, 2014-Semarang, Indonesia) as Paper #1, “Managing

Biosafety and Biodivesity of Food from Local to Global Industries”