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MCQ Cloning and Ge Process

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0% found this document useful (0 votes)
47 views6 pages

MCQ Cloning and Ge Process

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eastgatej1
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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BIOTECHNOLOGY – GENETIC ENGINEERING PROCESS

Here are 20 multiple-choice questions based on:


GENETIC ENGINEERING PROCESS (RECOMBINAT DNA)

1. What is genetic engineering primarily used for?


a) Altering the environment
b) Modifying the genome of living cells
c) Developing new computer software
d) Constructing buildings

2. What does genetic engineering result in?


a) Hybrid organisms
b) Genetically modified organisms (GMOs)
c) Artificial intelligence
d) Enhanced biodiversity

3. Which of the following is NOT a use of GMOs?


a) Breeding more productive crops
b) Producing drugs or hormones
c) Treating genetic diseases
d) Manufacturing electronics

4. What is one process used to produce GMOs?


a) Cellular respiration b) Recombinant DNA technology
c) Photosynthesis d) Fermentation

5. Which organism is commonly used to manufacture human insulin


through recombinant DNA technology?
a) Yeast b) E. coli bacteria
c) Arabidopsis thaliana d) Mice

6. What is the first step in the process of producing human insulin


using recombinant DNA technology?
a) Inserting the chosen gene into a plasmid
BIOTECHNOLOGY – GENETIC ENGINEERING PROCESS
b) Isolating the gene to make insulin
c) Cutting open bacterial DNA
d) Culturing the bacterium

7. Which enzyme is used to cut open bacterial DNA during


recombinant DNA technology?
a) DNA polymerase b) RNA polymerase
c) Restriction enzyme d) Ligase

8. What happens after the chosen gene is inserted into the plasmid
during recombinant DNA technology?
a) The plasmid is discarded
b) The bacterium is genetically modified
c) The gene is removed from the plasmid
d) The gene is amplified

9. What is the main purpose of culturing the bacterium after genetic


modification?
a) To study its genetic makeup
b) To create more bacteria
c) To analyze its protein expression
d) To release it into the environment

10. What is the ultimate goal of genetically modifying bacteria to


produce human insulin?
a) To cure bacterial infections
b) To produce insulin for medical use
c) To create new bacterial species
d) To study bacterial genetics

11. Which of the following is NOT a potential application of genetic


engineering?
a) Curing brain tumors b) Treating cystic fibrosis
c) Improving food quality d) Enhancing clothing materials
BIOTECHNOLOGY – GENETIC ENGINEERING PROCESS
12. What is the purpose of inserting the chosen gene into a plasmid
during genetic engineering?
a) To transfer it to another organism
b) To amplify the gene
c) To cut the gene into smaller fragments
d) To attach it to the bacterial chromosome

13. What term describes a bacterium that has been genetically


modified to produce a desired protein?
a) Transgenic b) Hybrid
c) Mutant d) Wild type

14. Which enzyme is used to attach the ends of the inserted gene to
the plasmid during genetic engineering?
a) DNA ligase b) DNA
polymerase
c) RNA polymerase d) Restriction
enzyme

15. What role does the plasmid play in genetic engineering?


a) It acts as a vector to carry the gene of interest
b) It serves as the primary source of genetic material
c) It regulates gene expression
d) It synthesizes proteins

16. What is the significance of culturing the genetically modified bacterium?


a) To create genetic diversity
b) To study bacterial evolution
c) To produce more bacteria with the desired trait
d) To prevent bacterial contamination
17. What is the primary advantage of using recombinant DNA
technology to produce human insulin?
a) It is cost-effective
b) It is environmentally friendly
c) It eliminates the need for insulin injections
BIOTECHNOLOGY – GENETIC ENGINEERING PROCESS
d) It reduces the risk of allergic reactions

18. Which of the following is NOT a step in the process of genetic


engineering to produce human insulin?
a) Isolating the gene to make insulin
b) Inserting the gene into the bacterial chromosome
c) Culturing the genetically modified bacterium
d) Cutting open bacterial DNA

19. How does genetic engineering contribute to medical


advancements?
a) By creating new animal species
b) By producing drugs and hormones
c) By increasing biodiversity
d) By enhancing computer technology

20. What is the primary purpose of genetically modifying bacteria to


produce insulin?
a) To improve agricultural productivity
b) To treat bacterial infections
c) To provide a sustainable source of insulin
d) To study bacterial genetics

Here are the answers to the 20 multiple-choice questions:

1. b) Modifying the genome of living cells


2. b) Genetically modified organisms (GMOs)
3. c) Treating genetic diseases
BIOTECHNOLOGY – GENETIC ENGINEERING PROCESS
4. b) Recombinant DNA technology
5. b) E. coli bacteria
6. b) Isolating the gene to make insulin
7. c) Restriction enzyme
8. b) The bacterium is genetically modified
9. b) To create more bacteria
10. b) To produce insulin for medical use
11. d) Enhancing clothing materials
12. a) To transfer it to another organism
13. a) Transgenic
14. a) DNA ligase
15. a) It acts as a vector to carry the gene of interest
16. c) To produce more bacteria with the desired trait
17. d) It reduces the risk of allergic reactions
18. b) Inserting the gene into the bacterial chromosome
19. b) By producing drugs and hormones
20. c) To provide a sustainable source of insulin

If you have any further questions or need clarification on any of the


answers, feel free to ask!
BIOTECHNOLOGY – GENETIC ENGINEERING PROCESS

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