Chemical Safety Guide

EID Hazard Identification
Globally Harmonized System GHS Pictograms:
The Globally Harmonized System of Classification HEALTH HAZARD FLAME EXCLAMATION MARK
and Labeling of Chemicals (GHS) provides an
international standardized approach to the
classification and labeling of chemicals. The
Occupational Safety and Health Administration
(OSHA) Hazard Communication Standard (HCS) • Carcinogen • Flammables • Irritant (skin and eye)
aligns with the GHS to ensure consistency of • Mutagenicity • Pyrophorics • Skin Sensitizer
• Reproductive Toxicity • Self-Heating • Acute Toxicity (harmful)
information. The HCS requirements include:
• Respiratory Sensitizer • Emits Flammable Gas • Narcotic Effects
• Target Organ Toxicity • Self-Reactives • Respiratory Tract Irritant
Hazard Classification: Provides specific criteria for • Aspiration Toxicity • Organic Peroxides • Hazardous to Ozone
classification of health, physical and environmental Layer (Non-Mandatory)
hazards, as well as classification of mixtures. GAS CYLINDER CORROSION EXPLODING BOMB
Labels: Chemical manufacturers and importers

are required to provide a label that includes a
harmonized signal word, pictogram and hazard
statement for each hazard class and category, • Gas Under Pressure • Skin Corrosive/Burns • Explosives
along with precautionary statements. • Eye Damage • Self-Reactives
• Corrosive to Metals • Organic Peroxides
Safety Data Sheets (SDS): Previously called,

Material Safety Data Sheets (MSDS), have a
• • ••
i t , I •
SKULL AND
CROSSBONES
specified 16-section format.
Signal Word: There are two signal words in the

GHS system - Danger and Warning. These signal
words are used to communicate the relative level • Oxidizers • Aquatic Toxicity • Acute Toxicity (fatal or toxic)
of hazard on both the label and the SOS, with
If you see old pictograms on chemicals, please contact DEP for disposal at (301) 496-4710
"Danger" indicating the more severe hazard. The
appropriate signal word is determined by the
hazard classification.
Information and Training: While the GHS does not

address training, the HCS requires that all
personnel working with chemicals be properly
trained to recognize and understand chemical
labels and safety data sheets.
Labeling
In compliance with GHS, chemical manufacturers • Secondary containers within which a chemical Full secondary container labeling includes the
and importers are required to provide a label that is stored. following requirements:
includes six elements: • Chemical waste (the word "waste" must
• Name of the chemical (legible in English)
1. Product identifier also appear on the label)
• Hazard warnings
2. Signal word □NOTE Containers of newly synthesized • Date of transfer to secondary container
3. Pictogram chemicals have unknown hazards. Label
• Preparer's name
4. Hazard statement these containers with hazard information as
best determined. It is important to include • Expiration date (if applicable)
5. Precautionary statment
the location (lab notebook, computer file) Please see Chemical Hygiene Plan for more
6. Product supplier for finding specific information, such as: information, Section V, page 9, https:j/ors.od.
nih.gov/sr/dohs/Documents/chemical-hygiene
• Chemical name, exactly as it appears
EPICHLOROHYDRIN @ ,;;\ on the outside of the container
-plan.pdf
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UN No. 2023
CAS No 106-89-8
• Molecular formula Safety Data Sheets
� • Molecular weight (if known) Safety Data Sheets (SOS) have replaced Material
Safety Data Sheets (MSDS) under the Globally
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V
1f swallowed. Toxic in contact with skin.
Causes severe skin burns and eye damage.
Peroxide Formers
• Must be double-dated with a received date
Harmonized System. They present details of
the chemical hazards in a standardized format
to improve clarity and comprehension.
May cause an allergic skin reaction. May cause cancer. and open date.
The SDS is to include the following
Do not breathe dust/fume/gas/mist/vapor/spray. Wear
Secondary Container/ sixteen sections in this order:
protective gloves/protective clothing/eye protection Solution Labeling 1. Identification
Fill Weight: 18.52 lbs. Lot number: A0323111323
Gross weight: 20 lbs. Fill Date: 1/15/2021 Secondary container labeling is required in the 2. Hazard identification
®
Jackson Chemical Company - City of Industry,
following instances: 3.
4.
Composition/information on ingredients
First aid measures
• When the secondary container will be used
Los Angeles, California, USA (323) 268-1815
for longer than one work shift 5. Firefighting measures
• When the person who transferred the chemical 6. Accidental release measures
Once in the lab, the user may not obscure or
leaves the work area 7. Handling and storage
remove any of the label elements from the
container. If the chemical is transferred into • When the person who transferred the chemical 8. Exposure controls/personal protection
a different container, the name of the chemical takes it to an alternate location where they 9. Physical and chemical properties
and any associated hazards must also be are no longer in direct possession of the 10. Stability and reactivity
placed on that container; The label must be chemical 11. Toxicological information
legible, in English, and prominently displayed
This labeling requirement includes flasks, 12. Ecological information
on the container.
beakers, spray bottles and test tubes containing 13. Disposal considerations
This includes: chemicals. If the chemical will not leave the
14. Transport information
• All chemicals transferred from original container supervision of the person who transferred it
during one workday, complete secondary 15. Regulatory information
• Any prepared solution
container labeling is not required. 16. Other information
EID Hazard Identification

■I Hazard Control/Hazardous Spill Procedure
Hazard Assessment
A chemical hazard assessment identifies the What does this entail? 7. Proposed controls, to include
hazards of the chemicals and processes used Elimination
in the laboratory. The assessment evaluates A chemical hazard assessment entails
gathering information about the properties and Substitution
the potential for risk and the severity of the risk
proposed use of a chemical or reagent. This Engineering controls
associated with the hazards. The assessment
determines measures to eliminate, minimize, information should include: Administrative controls
or control the hazards and potential risks. 1. SDS for the chemical or reagent Personal protective equipment
2. Description of work/activities/use 8. A specific written chemical standard
What needs to be done?
3. Storage requirements operating procedure (SOP) is required for
To perform a chemical hazard assessment, Particularly Hazardous Substances (PHS).
4. Disposal and environmental requirements
laboratory workers should examine their plan Reference appendix N of the Chemical
for an experiment and identify any chemicals 5. Health surveillance Hygiene Plan for a template SOP for this
with which they are not familiar. The SDS for 6. First aid/emergency procedures purpose.
each unfamiliar chemical should be reviewed.
SDS should be maintained by the laboratory in
Specific Chemical Hazard Template and Lab-Specific Chemical SOP Template
a central location, accessible either as a hard
copy or electronically.
The Specific Chemical Hazard SOP is used to outline the hazards of a chemical and to describe
Who does the Assessment? general procedures for working with it The Lab-Specific Chemical SOP is used to outline the chemical
hazards of a specific procedure/experiment and to elaborate on the specific protocol to be used
The principal investigator or supervisor with during the procedure/experiment. Both SOPs can be used for PHS and non-PHS chemicals.
. ..... .... ... • .. . ...... . ... ..

primary responsibility for specific hazard
<J. ID} Nat�al Institutes of Health • Office of Research Services • Division of Occupational Health and Safety (J. D} National Institutes of Health • Office of Research Se!v�s • Division of Occupational Health and Safety
training, performs the chemical hazard ' ... •
assessment. He or she may consult with Title: Soecific Chemical Hazard Temolate Title: Specific Chemical Hazard Template
Document No. I Document I Author's Branch: I Effective Document No. I Document I Author's Branch: I Effective
colleagues or safety specialists from the Paae(s): Date: Paae(s\: Date:
Revision: I Revision Date: I Reviewed/Aooroved bv: I Date: I Revision Date: I Reviewed/Aooroved bv: I Date:
. . . .... . .
I Date: I Procedure
Revision:
Division of Occupational Health and Safety
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Overseeing Official's Signature: Overseeing Official's Signature: Date: Procedure
I
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(OOHS). .. Location:
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Location:
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This template is intended to be filled out with information on chemical hazards
□ NOTE The Chemical Hygiene Plan contains within the laboratory. The purpose is to generate a chemical-specific standard D Specific Laboratory procedure or experiment [Examples: synthesis of
..
operating procedure which is to be followed by each individual handling or chemical luminescent esters, folate functionalization of polymeric micelles, etc.]
templates for performing chemical hazard potentially exposed to the given chemical. To fill out this template: D Generic laboratory procedure that covers several chemicals
Section 1: Use information from the chemical safety data sheet (SOS)
assessments and generating SOPs. Please
..
[Examples:distillation, chromatography, etc.]
Section 2: Describe the lab-specific procedure(s) the chemical is used in
see Appendix N of the NIH Chemical Hygiene Section 3: Attach the SOS for the chemical to this SOP D Generic use of specific chemical or class of chemicals with similar hazards
.
Section 4: Enter information on the ventilation controls used and/or any [Examples: organic azides, mineral acids, etc.]
Plan for more information. alternatives to standard ventilation controls 2 DESCRIPTION OF PROCESS/EXPERIMENT
.
Section 5: List the PPE that will be worn when working with the chemical.
Ensure that any PPE listed is compatible with the chemical. [Provide a brief description of your process or experiment, including its purpose.
Do not provide a detailed sequential description as this will be covered
.
Section 6: Fill out information on the preparation, usage, storage, and transport
of the chemical. Add details for lab-specific procedures. by section #6 of this template. Indicate the frequencey and duration below.]
Section 7: Fill in specific details for spill procedures. Include information such as Done time D daily D weekly D monthly
..
the location of spill kits, the specific materials that must be used, PPE that must □
Frequency
other:---
be worn, or specific procedures that must be followed during spill cleanup. Duration per
Section 8: No action necessary. experiment: - - minutes; or - - - -
- hours
..
Section 9: Fill in information on waste disposal - customize to the specific 3 SAFETY LITERATURE REVIEW AND HAZARD SUMMARY
procedure and chemical.
Section 10: No action necessary. 1. Hazardous Substances
.
Section 11: Fill in information on decontamination and designated work location
[List hazardous substances and their associated health and safety hazards.
Examples of potential hazards include toxicity, reactivity, flammability, corrosivity,
for the chemical. pressure, etc. Refer to Safety Data Sheets (SDSs) and other resources, as needed.]
Section 12: Document training for all personnel who will handle this chemical.
All personnel must sign to indicate they are aware of the hazards and of all 2. Other Hazards
general and lab-specific procedures which must be followed when using this {List nonchemical hazards, e.g., biological hazards, electrical hazards, physical hazards,
chemical. ffncluding sharps), mechanical hazards, nonanes radiation, or ionizing radiation.}
Hierarchy of Controls
Hierarchy of Safety Controls
Most
Physically
effective remove the hazard
Replace
Substitution the hazard
Isolate people
Engineering Controls from the hazard
Administrative Change the

way people work
Controls
Protect the workers with

Personal Protection Equipment
Least
effective

Control of Exposure
The best way to control or minimize For information about engineering that fails tests fixed promptly. Make sure
exposures is to eliminate the hazard. controls please see Section 5 all personnel know where the eye wash is
Ventilation located and how to use.
If a hazard cannot be eliminated, other hazard
control methods must be used. Personal • Call Office of Research Facilities (ORF)
For information about Personal
protective equipment (PPE) should never be the (301) 435-8000 to have the safety
only method used to reduce exposure because
Protective Equipment (PPE) please
showers tested annually. Submit a ticket
PPE may "fail" (stop protecting the worker) with see Section 6 - Personal Protective at https://58000.nih.gov/.
little or no warning. Equipment (PPE)
• Enter an online clearance request for the
□ NOTE Prevention is the most effective means lab with the Division of Occupational Health
of hazard control. Prepare your work area and Safety (OOHS) when non-laboratory
before beginning any work! personnel have to enter the laboratory to
perform services such as renovations or
Administrative controls: equipment repair. Routine services such as
• Ensure personnel are adequately trained on floor cleaning and trach collection do not
the chemicals and associated processes. require clearance.
• Never pipette by mouth. • Keep personal protective equipment,
Engineering Control: Chemical Fume Hood • emergency phone numbers, and fire
Transport laboratory chemicals using bottle
extinguishers readily available.
carriers, secondary containment, and/or
suitable carts.
• Follow the established procedures for the
Personal Protective Equipment:
decontamination and movement of • Protect your clothes and exposed skin by
scientific and medical equipment found in
wearing appropriate personal protective
the NIH Personal Property Management
equipment. Consult the PPE section for
Guide.
detailed information.
• In the event of a hazardous chemical spill,
• Do not wear PPE outside the laboratory.
immediately follow the hazardous material
spill procedure (see next page). • Upon completion of work remove gloves
• Store all food, beverages, cosmetics, and carefully and thoroughly wash hands and
medications outside the lab. forearms before leaving the laboratory.
Administrative Control: Standard Operating Procedures,
policies and guidance Dispose of used gloves properly.
• Keep all doors to the laboratory closed to
ensure proper fume hood performance and • Wear eye protection when a splash
directional airflow into the lab. potential exists.
• Ensure unimpeded access to safety □ NOTE Contact lenses may be worn, with some
showers, eyewash stations and exit exceptions, but these do not function as
pathways. protective devices. The NIOSH Current
• Intelligence Bulletin prohibits wear of contact
Test and flush eyewash stations weekly
lenses when working with acrylonitrile, methyl
documenting the date and initials of the
enechloride, 1,2 dibromo-3-chloropropane,
individual who performed the test. Call
Personal Protective Equipment: Gloves and goggles ethylene oxide, and methyline dianiline.
ORF (301) 435-8000 to have equipment
Spill Prevention: 5. The spill involves Particularly Hazardous Substances
(PHS). PHS include select carcinogens, reproductive
□ NOTE High-level hazard spills: Note that spills of
>100 mis of any material and spills of Particularly
toxins and chemicals that have a high degree of acute Hazardous Substances of ANY volume should be
Most spills are preventable. The following are some tips toxicity. Please refer to Section X of CHP. cleaned up by emergency personnel. Refer to site
that could help to prevent or minimize the magnitude of specific directions under the High-Level Hazard Spills
a spill: If you witness or are involved in a high-level "Alert" on this page for more specific directions.
hazardous spill situation:
• Substitute a less hazardous chemical whenever The following substances are very hazardous and
possible. 1. Leave the area, closing doors behind you. clean up must be performed by experienced
• Think through each step of your experiment carefully. personnel only:
2. Prevent others from entering the area.
• Order/use the smallest quantity of chemicals Aromatic amines, nitro compounds, organic halides,
3. Initiate first aid at the work site: bromine carbon, disulfide ethers, cyanides, hydrazines,
possible.
and nitriles.
• Use bottle carriers to transport all glass bottles • Eyes: Flush with eyewash for 15 minutes. For
containing chemicals. water reactive chemicals, consult the SDS for □ NOTE Breakthrough time for some nitrile gloves
proper emergency response prior to using may be quite short; use thick nitrile gloves or
• Order solvents and acids in poly-coated glass safety
the chemical. multi-hazard gloves such as Silver Shield®. Always
bottles. The protective coating on these bottles can
check the manufacturer's guide for compatibility.
provide containment if the bottle breaks. • Skin: Remove contaminated clothing. Use
• Use secondary containment when possible. closest emergency shower for 15 minutes. □ NOTE ALWAYS report to OMS in the event of
chemical exposure, no matter how minor.
• Do not store bottles near the edges of shelves and 4. Notify your supervisor as soon as possible.
bench tops.
5. Bethesda main campus: Report (Monday-Friday,
7:30 AM to 5:00 PM) to the Occupational Medical Low-Level Hazard Spills:
High-Level Hazard Spills: Service (OMS), Building 10, Room 6C306 or call
(301) 496-4411 as soon as possible. If life
threatening, call the Fire Department of your local As a general guideline, chemical spills that are <100 mis
[!Em Contact the Fire Department: NIH campus immediately. and within a lab or contained area (such as a bench top
Bethesda main campus: Dial 911 from an office phone or inside the chemical fume hood) AND don't involve
or (301) 496-9911 from a cell phone; Off-campus: Dial
RML: Report (Monday-Friday, 8:00 AM to 4:30 PM) PHS, are considered to be low-level spills. HOWEVER,
to RML OMS, Building 5, Room 204 or call (406) DO NOT attempt to clean up a spill unless you have the
9-911 from an office phone or 911 from a cell phone.
375-9600 as soon as possible. If life-threatening, training and resources to clean the spill with no risk to
For satellite locations, follow satellite-specific guidance
call O from an office phone or 911. yourself or others. Attempt ONLY if it is non-volatile liquid
which supersedes the main campus guidance at
these locations. with which you are familiar, and you have appropriate
!RF-Frederick: Report (Monday-Friday, 7:30 AM
supplies on hand, including:
RML: Contact the Security Control Center (SCC). Dial 0 to 5:00 PM) to IRF OMS, Room 1B116 or call
from an office phone or (406) 363-9400 from a cell (301) 631-7233 as soon as possible. If life • Personal protective equipment: safety goggles,
phone. threatening, call 911. chemical resistant gloves, lab coats, face shields.
!RF-Frederick: Call on-site OMS Clinic or follow Baltimore: Report (Monday-Thursday, 8:30 AM to • Spill kit or absorbent, compatible material to
emergency response plan. 4:30 PM; Friday, 7:30 AM to 3:30 PM) to Baltimore contain the spill.
Baltimore: Refer to the Baltimore chemical spill SOP OMS: BRC, Room 18210 or call (443)740-2309 as
• Disposal container (or bag) to collect absorbent
for guidance. soon as possible. If Baltimore OMS is unavailable
material. Dispose as chemical waste.
during normal working hours, contact Bethesda
A high-level hazard spill is defined as one that requires
OMS at (301) 496-4411. RML: Contact the Security Control Center (SCC).
an emergency response. If ANY of the following criteria
Dial O from an office phone or (406) 363-9400 from
are met, an emergency response must be initiated: For all campuses, if OMS is closed, call the
from a cell phone.
Clinical Center Operator at (301) 496-1211 and
1. The employee is uncomfortable.
ask them to page an OMS physician immediately. Baltimore: Refer to the Baltimore Chemical Spill SOP
2. Assistance from outside the immediate release For alternate satellite locations.report to your on
area is required. site OMS Clinic (if applicable) or follow your satellite Other satellite locations: Follow satellite-specific
emergency response plan. guidance, if applicable.
3. The incident is likely to result in an uncontrolled
release of hazardous substances (to drains, to 6. Do not re-enter the room until the Fire Department For more information consult the NIH Chemical
the air, etc.). or appropriate authorities determine that the area Hygiene Plan at https://ors.od.nih.gov/sr/
is safe. dohs/Documents/chemical-hygiene-plan.pdf
■I
4. Response to a release poses a potential safety
or health hazard to the responder.
Hazard Control/Hazardous Spill Procedure

Chemical Storage
Proper chemical storage can be a challenge in Store flammable and combustible materials Store bases in a corrosives cabinet.
the limited space of laboratories. The hazards in an approved storage cabinet. Flammables
associated with chemical storage can be should be stored in an approved flammable Store highly toxic materials in a closed,
reduced through prudent purchasing, handling storage cabinet away from chemical fume hoods dedicated poison cabinet.
and disposal practices. and biological safety cabinets. Containers of
500 mis or less of flammables of current day Do not use the work surface of chemical fume
Considerations for proper storage: working stock may be stored outside a flammable hoods to store containers and equipment, as
Ensure all hazardous chemicals are properly cabinet. Flammable working stock cannot be this prevents proper air flow, reduces available
labeled. stored outside of flammables cabinet when not work space, and may increase hazards in case
working with it. Keep cabinet doors closed. of fire or spill.
Record the date of receipt on each bottle to
assist with inventory management. Always store hazardous chemicals no higher ✓ TIP
Use work surface of a shelf to hold
than eye level and never on top of a storage
Record the date of opening on each peroxide supplies inside a fume hood to avoid blocking
unit. Do not overcrowd shelves.
former and dispose prior to the expiration date. the rear baffle and disrupting proper operation
See the Chemical Hygiene Plan, Appendix D, of the hood.
Do not store chemicals on the floor.
for more information.
Liquids should be stored on shelves with Chemicals to be stored in a refrigerator or
Upon preparation, label and date solutions
a lipped edge and in spill trays to hold the freezer must be in units appropriately rated for
with the chemical or mixture name, as well as
contents if the container breaks or leaks. hazardous material storage.
any applicable hazard warnings. See additional
information in Hazard Identification/Labeling Store acids in a dedicated acid cabinet, NEVER store hazardous chemicals in a cold
section. preferably in the ventilated storage area room or other storage area with recirculating
Store incompatible chemicals separately beneath the chemical fume hood. Nitric, ventilation.
by hazard class. Within a hazard class, perchloric, chromic and sulfuric acids are
chemicals may be stored alphabetically. strong oxidizers and must be kept isolated
from organic acids.
ACID
STORAGE
Store acid in a ventilated corrosive cabinet Store bases in a corrosive cabinet Store chemicals on spill trays to contain leaks or drips
Please consult Appendix D in the NIH Chemical Hygiene Plan for more information, https//ors.od.nih.gov/sr/dohs/Documents/chemical-hygiene-plan.pdf
Chemical Storage (continued)
• Do not store hazardous chemicals under a sink. Chemical Transport

• Secure all compressed gas cylinders. Keep Moving chemicals within your laboratory:
protective caps on cylinders when not in use • Inspect each container's cap or closure
(including during transport). Remove empty seals for the formation of crystals.
cylinders as soon as possible.
1 • • Do not tighten, open or move containers
• Store chemicals away from heat and direct that have crystals forming on the caps and
sunlight. seals or inside the bottle in case they are shock
sensitive; on Bethesda main campus notify your
• Rotate chemical inventory. Dispose of
IC safety specialist and supervisor immediately.
chemicals by their expiration date.
Contact contact chemical waste services im
• Inspect chemical containers regularly for mediately for removal at (301) 496-4 710. If
Inspect bottle caps for crystals.
deterioration and integrity. believed to be an emergent situation (picric
acid, diethyl ether, organic peroxides), express Moving chemicals outside of your lab:
• Store chemicals under appropriate conditions. this when placing the call.
Chemicals are stable only when stored in an Chemical transportation services are available
inert gas such as nitrogen. Certain chemicals RML: Contact the IH at (406) 802-6398.
through the DEP. Please contact DEP (301)
may burst into flame when exposed to air or !RF-Frederick: Call DEP at (240) 236-9575. 496-7990 and your OOHS safety specialist
moisture in the air. See the SOS for guidance. Baltimore: Call DEP at (301) 346-6287. for guidance when you are planning a laboratory
move or before you move any chemicals.
• Consult safety references (e.g., SOS) before Consult the CHP, Appendix D, for more detailed
working with chemicals that are new or information. RML: Chemical transportation services are
unfamiliar. available through the RML HAZMAT Team. If
• Use carts or bottle carriers to move you are planning a laboratory move or before
chemicals short distances. moving chemical stocks, please contact the
Considerations For Ordering: HAZMAT Team Lead at (406) 363-9219 and the
• Purchase less hazardous alternatives RML Industrial Hygienist (IH) at (406) 375-7467.
whenever possible. Baltimore: Contact DEP at (301) 346-6287 or
[email protected] and chemical technicians
• Keep a chemical inventory to avoid for your IC.
duplicate purchases.
• NIDA: [email protected].
• Purchase chemicals in reasonable • NIA: [email protected]
quantities that will be used within six
months of purchase.
• Place chemicals in sturdy secondary Proper use of chemical bottle carrier
containers
• Place chemicals in sturdy secondary
□NOTE If an old or expired container of a peroxide containers
-forming chemical or reactive is found, do not
move it. On Bethesda main campus contact
the Division of Environmental Protection (DEP)
at (301) 496-4710 for assistance in disposing
of the container.
RML: Contact the Industrial Hygienist (IH) at
(406) 802-6398.
!RF-Frederick: Contact DEP at (240) 236-9575.
Baltimore: Contact DEP at (301) 346-6287 or

[email protected]. Sturdy secondary containers on carts are used to
Place chemicals in sturdy secondary containers transport chemicals safely
Chemical Waste Disposal
Waste disposal procedures are described in detail in the NIH Waste Disposal Guide (https://orf.od.nih.gov/EnvironmentalProtection/WasteDisposal/
Documents/NIH-Waste-Disposal-Guide-2022-508Ready.pdf. Chemical waste disposal guidance tool (https://nems.nih.gov/Documents/
Chemical_Waste_Tag_Guidance.pdf). Below are general guidelines:
DON'T: DO:
Consider an annual chemical clean-up day
Don't mix incompatible chemicals. Keep waste containers closed at all times. to dispose of old or unwanted chemicals.
Don't discard chemicals in sinks, medical Store waste in laboratory while awaiting • Segregate acids and bases, whether dry or liquid,
pathological waste (MPW) boxes, or general pick up. when collecting chemicals for pick up or when
trash. cleaning up the laboratory.
Place liquid waste containers in secondary
Don't treat chemical waste in any manner, containers (pans). • Flammables should be stored in their own box for
including use of ethidium bromide filters. chemical waste pick up (separate from acids and
Have chemical waste picked up within 60 bases). Exceptions exist for methanol/acetic
Don't dispose of volatile chemicals by days of accumulation start date. acid/water solution and triethylamine (please
evaporation. call DEP for guidance).
Dispose of empty chemical bottles as
Don't put waste containers in hallways or chemical waste or recycle. • Leave original labels on bottles whenever possible.
other public locations.
• If the researcher cannot identify a chemical,
Use only the safety cans provided by the please keep the unknown chemical(s) separated
Don't move chemicals with precipitation on
chemical disposal service for disposing of from other chemicals.
or in the bottle.
flammables.
• Affix a Chemical Waste tag to each box, identifying
□NOTE Picric acid is a commonly used reagent in Attach and complete a chemical waste tag contents.
biological laboratories which becomes a dangerous to ALL chemical waste not in its original
explosive if allowed to crystallize. If dehydrated • Call DEP for pick-up:
container.
picric acid is discovered, DO NOT MOVE THE Bethesda main campus: (301) 496-4710
BOTTLE. Call DEP for removal. Picric Acid is a Contact DEP or your OOHS Safety Specialist RML (HAZMAT team): (406) 363 -9219
Particularly Hazardous Substance (PHS). Consult if you are unsure how to dispose of a
the CHP, Appendix D, for more detailed information. chemical. !RF-Frederick: (240) 236-9575
Baltimore: (301) 346-6287
Pleaserefer to the NIH Waste Disposal Guidefor

further information, https:j/orf.od.nih.gov/
*Certain items may require special handling based on the stability of the material.
EnvironmentalProtection/WasteDisposalj
These items may include peroxide formers, explosives, water reactive chemicals, as well Documents/NIH-Waste-DisposaI-Gu
as shock, air and temperature sensitive items. CALL DEP FOR REMOVAL OF THESE ITEMS. ide-2022-508Ready.pdf.
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Chemical Waste Tag and examples of correct disposal techniques

Highly Reactive Chemicals
Highly reactive chemicals include those which Organic Peroxides are extremely sensitive to Peroxide forming chemicals must be labeled
are inherently unstable and susceptible to rapid light, heat, shock, sparks and other forms of with the date of receipt from the manufacturer
decomposition. Also included in this category are accidental ignition, as well as to strong oxidizing and the "opened" date.
those chemicals which can react alone or with and reducing materials. All organic peroxides
other substances in a violent uncontrolled are highly flammable. Peroxides may deteriorate □NOTE Avoid the distillation, concentration,
manner, liberating heat, toxic gases, or leading quickly. Always dispose of peroxides upon or evaporation of peroxide formers by first
to an explosion. expiration. testing for the existence of peroxides.
Peroxide detection test strips are available
Air, light, heat, mechanical shock (when struck, Peroxide Formers can form peroxides from most lab equipment supply companies.
vibrated or otherwise agitated), water and spontaneously during storage and especially
certain catalysts can cause decomposition of Chemicals that are sensitive to peroxide
after exposure to the air. This is particularly formation can be broken into three categories
some highly reactive chemicals, and initiate dangerous if:
an explosive reaction. Hydrogen and chlorine shown in the following table
may react explosively in the presence of light.
Alkali metals, such as sodium, potassium and
• Peroxides are present during a distillation,
where applied heat to the concentrated
&CAUTION
lithium react violently with water liberating PEROXIDE
solution may trigger a violent explosion. FORMING
Label peroxide
forming chemicals
hydrogen gas. Examples of shock sensitive � CHEMICAL with "received" and
materials include acetylides, azides, organic • Peroxides evaporate, leaving the crystals of
--+
Date Received: _____ "opened" dates
Date Opened: _ _ _ _ _
nitrates, nitro compounds and peroxides. highly explosive peroxide at the bottom of Date Expires: _____
When working with highly reactive chemicals, the container. Inhibitor Added: Yes No
For more ln!o,matlon p�se see tile Chemical Hy�en• Plan
employees should conduct a Chemical Hazard

Assessment.
□NOTE For more information consult the NIH Chemical Hygiene Plan at https://ors.od.nih.gov/sr/dohs/Documents/chemical-hygiene-plan.pdf
Suggested Storage Time Limits for Common Peroxide Forming Compounds
CLASS A: MOST DANGEROUS CLASS B: DANGEROUS CLASS C: DANGEROUS

Chemicals that can form explosive These chemicals are a peroxide hazard during storage Unsaturated monomers that may auto-
levels of peroxides during storage and on concentration (distillation/evaporation). A test polymerize as a result of peroxide
without concentration. for peroxide should be performed if concentration is accumulation if inhibitors have been
intended or suspected. removed or are depleted.
Discard after 3 months. Inspect every 6 months following date of opening Inspect every 6 months following
date of opening
lsopropyl ether Acetal Dioxane Acrylic acid

Butadiene Cumene Ethylene glycol Butadiene
Chlorobutadiene (chloroprene) Cyclohexene Furan Chlorotrifluoroethylene
Potassium amide Cyclooctene Methyl acetylene Ethyl acrylate
Potassium metal Cyclopentene Methyl cyclopentane Methyl methacrylate
Sodium amide Diacetylene Methyl-isobutyl ketone Styrene
Tetrafluoroethylene Dicyclopentadiene Tetrahydrofuran Vinyl acetate
Divinyl acetylene Diethyl ether Tetrahydronaphthalene Vinyl chloride
Vinylidene chloride Diethylene glycol dimethyl ether Vinyl ethers Vinyl pyridine
ma
Adapted from Prudent Practices in the Laboratory: Handling and Disposal of Chemicals, Updated Version, National Research Council, 2011.
Special Hazards
ma Special Hazards
Safe Management of Particularly Hazardous Substances in NIH Laboratories
Proper chemical storage can be a challenge in appropriate protective measures and receive Then, if a substance meets any of the above
the limited space of laboratories. The hazards training when working with any PHS. The DOHS criteria, the Pl must consider whether its use
associated with chemical storage can be reduced Chemical Hygiene Officer (CHO) and safety poses a significant hazard. For instance,
through prudent purchasing, handling and specialist for your institute (301-496-2960)
disposal practices. are available to assist upon request. • Is the chemical handled rarely, occasionally,
or repeatedly?
RML: Contact the RML IH (406) 802-6398).
What are Particularly Hazardous
!RF-Frederick: Contact IRF-DOHS at • Is the procedure done on the open bench, in a
Substances (PHS)? laboratory hood, or in an enclosed apparatus?
(301) 631-72 44.
PHS are chemicals that may pose extreme
• Is there a potential for the employee to be
hazards to laboratory employees. As defined by My laboratory uses numerous hazardous exposed to the chemical?
the OSHALaboratory Standard (29CFR 1910.1450) chemicals. How do I determine which
https://www.osha.gov/laws-regs ones must be classified as PHS? • Does the chemical's use pose a significant
regulationsstandardnumber/1910/1910.1450. potential for exposure through any route
PHS include substances with a high degree of First, review each chemical's properties. (inhalation, skin, or ingestion)? For instance, is
acute toxicity, reproductive toxins, and select Chemicals that may be considered PHS it highly volatile? Are dusts or aerosols formed?
carcinogens (those strongly implicated as a include: Is it heated or concentrated?
potential cause of cancer in humans and/or • substances that may cause severe, acute,
those regulated by OSHA, https:j/ • Is this a reproductive hazard? Reproductive
or lethal effects through any exposure, hazards are substances or agents that may
www.osha.gov/carcinogens/ standards). OSHA route (inhalation, injection, skin absorption,
and NIHrequire the development of protocols for affect the reproductive health of women or
or ingestion) in quantities of 50 micrograms men or the ability to have healthy children. OMS
the identification and management of PHS. per kilogram of body weight (50 µg/kg) or may be consulted by all staff of reproductive
less; capability (i.e., women, men, those who are
• highly unstable or explosive materials, alone pregnant, planning to become pregnant, or
or in combination with other materials; breastfeeding) when there is a concern for
past exposure or potential future exposure
DESIGNATED AREA • carcinogens; to a reproductive hazard(s).
for select carcinogens, reproductive
toxins and high acute toxicity chemicals • reproductive toxins; • Do any employees have known chemical
AUTHORIZED PERSONNEL ONLY sensitivities?
• materials that may generate acutely toxic
by-products that could overwhelm control • Is there a potential for new or unknown sub
Who is responsible for identifying and measures or penetrate personal protective stances to be created during the procedure?
mananging PHS in NIH labs? equipment;
• Are the signs and symptoms of exposure
The Principal Investigator (Pl) has primary • substances that have been determined by readily apparent?
responsibility for determining which chemicals DOHS to present a unique hazard or are used
must be handled as PHS. Each Pl is also in an operation that requires approval above
responsible for ensuring that employees follow the level of the laboratory supervisor.
For more information on how to determine if a chemical is a PHS, consult the NIH Chemical Hygiene Plan ( Appendix B) at https://ors.od.nih.gov/sr/
dohs/Documents/chemical-hygiene-plan.pdf#search=Chemical%20Hygiene%20Plan.
Once I have identified all the PHS in □ NOTE Commonly used PHS are chloroform, Safe Management of Particularly
my lab, what do I do next? formaldehyde, sodim azide and ethylene oxide. Hazardous Substances in NIH
PHS examples that have antidotes or prophylaxis Laboratories
are hydrogen fluoride, MPTP, phenol and hydrogen
1. Determine whether or not the chemical can be
cyanide. Please refer to the NIH Chemical Hygiene
replaced with a less hazardous chemical.
Plan for further information, https://ors.od.nih.gov/
2. If it cannot be replaced, follow the steps below sr/dohs/Documents/chemical-hygiene-plan.pdf
to conduct a hazard assessment and create
a PHS specific SOP. When PHS are used for the □ NOTE Medical counseling through the Occupational
• Severe. acute, or lethal effects?
first time, perform a chemical hazard analysis Medical Service (OMS) is offered for emp loyees • Is 1t a carcinogen?
• Is 1t a reproductive toxin?
using the Specific Chemical Hazard template working with hazardous chemicals. OMS provides • High level of acute tox1c1ty?
• Other determination of unique hazard by NIH?
in Appendix N of the Chemical Hygiene Plan. additional services to employees working with • Highly unstable or explosive?
Assistance is available from the IC Safety potentially hazardous chemicals that monitor
Specialist and the DOHS Chemical Hygiene individuals for adverse health effects and
I
Officer (CHO). If the hazard analysis identifies determines treatment strategies that can help to
that the PHS has antidotes or prophylaxis prevent or significantly reduce adverse health
Yes to one or more questions No to all questions
available, the CHO will consult with OMS effects if exposed. These include medial surveil
to facilitate required medical services (e.g., lance and post-exposure treatment and follow-up
counseling for laboratorians before they programs.
•
commence work with the PHS or hazard
specific incident response review for OMS Where can I get more information?
staff). For detailed information, consult the For assistance with PHS or any other lab safety
NIH Chemical Hygiene Plan. questions, contact the DOHS at (301) 496-2960.
3. Once the PHS has been identified, then the RML: Contact the RML IH at (406) 802-2960.
Pl will create their own PHS lab-specific IRF-Frederick: Contact IRF-DOHS at (301) 631-7244.
Standard Operating Procedure (SOP) with • Is it OSHA regulated (required standards)?
NIA Labs: Contact NIA Safety at NIAIRP
available assistance from their Safety
Baltimore [email protected]. • Does it have severe, acute or lethal effects?
Specialist and the DOHS CHO using the Lab
-Specific SOP in Appendix N of the Chemical NIDA Labs: Contact NIDA Safety at NIDASafety • Is it a carcinogen or reproductive toxin?
Hygiene Plan to include: @mail.nih.gov.
• Does it have acutely toxic by-products?
• description of the PHS See also:
• Does it present a unique or significant hazard?
• a step-by-step review of the work involving NIH Chemical Hygiene Plan (CHP),
(Consult DOHS if needed)
https://ors.od.nih.gov/sr/dohs/
the PHS Documents/chemical-hygiene- • For more info and how to identify PHS see
• the engineering controls and PPE for the PHS plan.pdf#search=Chemical%20Hygiene Appendix B of the NIH CHP
%20Plan.
• special handling procedures and storage • Identify all potential physical and health effects
requirements National Research Council, Prudent Practices in the Laboratory: • Perform a step-by-step review of the work
• spill and accident procedures Handling and management of Chemical Hazards, National
Academy Press https://nap.nationalacademies.org/ • Identify engineering controls, work practice
• exposure procedures in case of an emergency catalog/12654/prudent-practices-in-the-laboratory-
handling-and-management-of-chemical
and PPE to be used
• waste collection and disposal • Establish a clearly marked, designated area
• designated area and decontamination OSHA standard 29 CFR 1910. 1450. for the PHS
procedures. Occupational exposure to hazardous
chemicals in laboratories, https://www. • Identify decontamination procedures (see
4. Once the hazard analysis, SOP and OMS osha.gov/laws-regs/regulations/standard "Hazard Control" section for more detail)
number/1910/1910.1450
consultation, if applicable, have been • Write a PHS-specific safety protocol
completed, the laboratory can begin to work
Appendix B of the OSHA laboratory • Ensure primary barriers (e.g., chemical fume
with the PHS safely.
standard(1910.1450) contains a list of hoods, downdraft tables, etc.) are functioning
5. The supervisor can approve the use of the references, at https://www.osha.gov/laws- properly
regs/regulations/
PHS only after employees have been trained • Ensure availability and use of appropriate PPE
standardnumber/1910/1910.1450AppB.
•
according to the safety protocol per the PHS
requirement. Document that all employees
who work with any PHS have received proper
'" OSHA Laboratory Safety Guidance
• Train employees according to the safety protocol
• Document training
booklet, OSHA 3404-11R, 2011, available
training and a copy of the written safety at https://www.osha.gov/sites/default/
protocol. • • I files/publications/OSHA3404laboratory-
l!i . . safety-guidance.pdf
ma Special Hazards
ma Special Hazards
Toxic Chemical Reduction Initiative
NIH developed the Toxic Chemical Reduction environmentally friendly than their more • Health and safety risk
Initiative in conjunction with the requirement hazardous counterpart. This venture was • Quantity generated
for strict control of Particularly Hazardous spearheaded by the Division of Environmental
Substances, and in response to the mandate of Protection through the NIH Environmental • Mandated regulatory reduction
Executive Order 13514 and NIH's environmental Management System (NEMS). About 200 • Alternative availability and feasibility
goal to reduce toxic and hazardous chemical toxic chemicals were scored numerically and
(For more information about this initiative,
use, NIH developed the Toxic Chemical ranked based on the following four criteria
visit the NIH Environmental Management
Reduction Initiative. This initiative identified for reduction:
System (NEMS) website at https://
alternatives that are less toxic and more
nems.nih. gov/environmental-programs/
Pages/default.aspx)
Compressed Gases
Hazards associated with compressed gases Handling

include:
Be familiar with the hazards of the compressed
• Oxygen displacement gas (consult the SDS).
• Fires Use a suitable hand truck or cart equipped with
a chain or belt for securing the cylinder to the
• Explosions
cart, even for short distances.
• Toxic gas exposures
Cylinder caps should be secured on each
• Physical hazards associated with high cylinder unless in use. Cylinder caps protect
pressure systems the valve on top of the cylinder from damage.
Never tamper with pressure relief devices in
Special storage and handling precautions are valves or cylinders.
necessary to control these hazards. Use a crescent wrench, not a pipe wrench
(it roughens the edges of the nut), when
Storage
manipulating the regulator.
All cylinders must be secured to a wall, bench
or fixed support using a chain or strap placed Keep the cylinder valve closed except when in use.
at 2/3 the height of the cylinder. Cylinder stands
Use compressed gases only in a well-ventilated
are an alternative to straps.
area; never use or store them in a cold room.
Store cylinders in cool, well-ventilated, fire Cold rooms are built to be closed systems without
resistant areas in compliance with local, state, ventilation and expose the user to risk of asphyx
and federal regulations. iation if compressed gases are used.
Separate flammable gases from oxygen by a Toxic, flammable and corrosive gases should be
minimum distance of 20 feet. handled in a chemical fume hood.
Where more than one type of gas is in use, label

Do not store full and empty cylinders together.
gas lines.
Keep the number of cylinders in the laboratory
Inspect valves and tubing regularly for wear and
to a minimum and ensure that the cylinder
tear, and for leaks using the soap bubble test.
contents are properly and prominently labeled.
Keep empty compressed gas cylinders closed, Use the appropriate regulator for the cylinder;
capped, secured, and stored so that they may never substitute another regulator.
be removed with a minimum handling of other For more information, visit us at the OOHS
Don't use oil or grease to seal the 0-ring on the
cylinders. Oxygen Monitoring Devices website at
regulator. https//ors.od.nih.gov/sr/dohs/safety/
Empty cylinders should be stored so that they Pages/oxygen.aspx
may be removed easily.
Cryogenic Liquids and Solids
Nitrogen and helium determine if an oxygen monitoring device is simple asphyxiant. Safety measures for
needed in your location, call the DOHS Oxygen handling and transporting the material include:
Nitrogen and helium are the most commonly
Monitoring Program Mananger for an assessment
used cryogenic liquids. Liquid nitrogen (LN2) is • Wear protective clothing. Use insulated
on Bethesda main campus at (301) 496-2960.
commonly used for storing laboratory samples gloves that are made of a thick material;
RML: Call the IH at (406) 363-9429.
and/or components; liquid helium may be also wear proper clothing (long-sleeves and
!RF-Frederick: Call any IRF-DOHS staff for
used for cooling superconducting magnets. pants) to be sure that no skin is exposed.
assistance.
Both are extremely cold (-195.8°C/-384.4°F
for nitrogen and -268.9°C/-516 °F for helium) These devices require preventive maintenance • If possible, use tongs while wearing gloves
while in a compressed, liquid state. In their and calibration on an annual basis, or as specified instead of your properly gloved hands to pick
gaseous state, they are inert, colorless, odorless, by the manufacturer. There is also a DOHS up dry ice.
noncorrosive and nontoxic. protocol for using these devices.
• Handle the dry ice in a well-ventilated room
rimD Cryogen contact with skin may cause For more information, visit the Oxygen Monitoring and NEVER use dry ice in a cold room.
Devices section on the DOHS website at https://
serious freezing (frostbite) injury. Wear cryo • Do not place dry ice in an airtight container;
ors.od.nih.gov/sr/dohs/safety/Pages/oxygen.aspx
protective insulated gloves when working the pressure caused by the dry ice
with cryogenic liquids. Cryogenic gloves used
Oxygen monitoring devices: sublimating may cause the container to
in research are not designed/rated to protect explode or rupture.
against immersion into cryogenic liquids or • are typically installed in locations where
prolonged handling of cryogenically chilled compressed gases or cryogenic liquids are • Do not dump dry ice down the drain as it
materials. Seek training to safely work with stored and/or dispensed in a manner that may damage the sink or plumbing; allow it
cryogens and liquid nitrogen. Consult manu would create the potential for displacement to sublimate at room temperature, ideally in
facturer's instructions before using. For more of oxygen a CFH or local exhaust device or if unavailable,
in a large well-ventilated space.
• may be required by the NIH Design
information about cryogen safety, including
Requirements Manual for Biomedical

how to respond to a cryogen-related injury,
Laboratories and Animal Research Facilities

please see the DOHS Cryogen Fact Sheet.
When liquid nitrogen or helium are dispensed • alarm occupants of dangerously low oxygen
they release gases, which act as simple levels
asphyxiants and displace oxygen. Displacing
• must be serviced and maintained by the
oxygen creates the potential for a hazardous
NIH Institute or Center, including calibration
environment. OSHA specifies that a hazardous
every six months (or more frequently if
atmosphere may include one where the oxygen
recommended by the manufacturer)
concentration is below 19.5% or above 23.5%. ■BSR
600
The following are some of the most common rimD If the device alarms, evacuate the UltraShield'"
locations where cryogenic liquids are found, space and call 911 (off campus call 9-911)
potentially creating an oxygen deficient to have the Fire Department verify oxygen
atmosphere: levels.
RML: Call the sec at (406) 363-9400.
• Magnetic Resonance Imaging (MRI) rooms
(or magnet rooms) IRF-Frederick: Call any IRF-DOHS staff for
• Nuclear Magnetic Resonance (NMR) assistance.
Spectroscopy rooms Baltimore: Call Facilities
• GSH (BRC): (443) 740-2766
• Liquid Nitrogen Tanks (freezer farms)
• Camelot (Triad): (410) 558-9797. Liquid nitrogen tanks
• Transmission Electron Microscope (TEM)
rooms Cryogenic solids
Oxygen monitoring devices may be needed Dry ice is a cryogenic solid and should be
in these areas to continuously monitor oxygen treated in a manner different than regular ice.
levels where cryogenic liquids are stored. To Dry ice is extremely cold, solid CO2 and is a
rll Special Hazards

1§11 Ventilation
Chemical Fume Hoods and Other Local Exhaust Ventilation
The best way to prevent or reduce exposure by avoiding sharp curves that can create
to hazardous airborne chemicals is through turbulence.
the use of ventilation systems. Local exhaust Class II Type B biosafety cabinets provide
ventilation (LEV) provides containment of protection from biohazardous materials as well
airborne hazards at the source and directs as small quantities of potentially hazardous
vapor-producing chemicals. Air is pulled through
contaminated air away from the work area.
the cabinet and a HEPA filter and exhausted
All LEV must be certified by DOHS when outside the building. If considering a Type B
installed and on an annual basis. biosafety cabinet for your work, contact D0HS
(301-496-2960) to discuss suitability.
A chemical fume hood (CFH) is a ventilated
✓TIP Use Biological Safety Cabinet (BSC)
enclosure in which gases, vapors and fumes
Surface Decontamination Guidance for Use a tissue to check for proper airflow.
are exhausted. A fan on the roof of the building proper procedures. https://ors.od.nih.gov/sr
pulls air and airborne contaminants through dohs/safety/laboratory/BioSafety/Pages/ih_
the hood and ductwork and exhausts them to biocabinets.aspx
the atmosphere. Downdraft Tables. Like chemical fume hoods,
✓TIP Place a shallow shelf at the back of the downdraft tables exhaust air to the outside
atmosphere. Unlike chemical fume hoods, the
hood to hold supplies - this helps ensure blower is usually mounted below the work area
proper airflow. so that air is pulled down through a perforated
surface and then exhausted to the outside.
The CFH sash provides protective shielding
Downdraft tables are used for applications
and maximizes hood performance by
involving heavier-than-air gases and materials
optimizing the velocity of the air through the such as anesthetic gases and histological
front. Keep the sash at the appropriate level chemicals.
(as indicated by the green sticker- preferred
Other Local Exhaust Ventilation, (e.g., slot
height= 18", lowest allowed= 8") to ensure
hoods, snorkels and cage changing tables)
optimal face velocity. Improper positioning of must be certified when installed and on an
the sash can result in loss of containment annual basis.
(i.e. if the sash is too high, air velocity will be
insufficient, if the sash is too low, air velocity □NOTE Ductless CFHs are not permitted to be
used in NIH laboratories. Captured organic Downdraft table
will be too high, resulting in turbulence which
vapors may desorb from the charcoal filters,
can force contaminants out of the hood). All
resulting in capture failure.
work in a CFH should be conducted at least
6" inside {past) the sash. Close the sash when Parts of Chemical Fume Hood
the CFH is not in use.
The baffles direct the air being exhausted, -s

and in many hoods, they can be adjusted to ---7
improve performance. It is important that the I I
baffles are not closed or blocked since this ;�
blocks the exhaust path.

♦
♦ -
rn 0
□NOTE When working with heavy vapors such Airfoil ----------/Fc:;::::::;==::::::;z:::::'.,J
as halogenated solvents, consult with DOHS screen
�Work Surface
-
for optimal adjustment of the baffles.
- -
The airfoil or beveled frame around the hood Class II Type B biosafety cabinet
face provides more even airflow into the hood Front view Side view
Negative Airflow
In any laboratory where hazardous materials are handled, the air must flow from outside of the laboratory into the laboratory. It is critical to keep doors
closed to maximize ventilation efficiency. The laboratory's negative airflow serves as a secondary barrier to chemical hazards by keeping hazardous
airborne particles and noxious odors inside the laboratory.
Cold Rooms
Overview Cold room chemical hazards Recommendations for cold room work
Cold rooms have closed air-circulation systems Compressed gas, dry ice, liquid nitrogen and • Ensure door release mechanisms are working
and re-circulate any vapors from spills and leaks liquid helium can pose an asphyxiation hazard and doors are free of obstructions.
within the chamber. by displacing oxygen and should never be used • Avoid working alone. If you must work alone,
or stored in a cold room. let others know you are working inside and have
The refrigeration coils in cold rooms are someone check on you periodically.
aluminum and subject to damage from Flammable chemicals can release sufficient • Take a 10-minute break every 50 minutes while
corrosive atmospheres. vapors to form explosive atmospheres. Cold working inside the cold room.
rooms have fans and electrical equipment that
Electrical equipment used in cold rooms: • Ensure that shoes have no-slip soles; water
are potential ignition sources. and ice are common in cold storage and pose
• Should have Ground Fault Circuit slip and trip hazards.
Interrupter (GFCI) protection, as cold Noxious fumes may be released by materials
that have been heated on a hot plate. Only use • Dress appropriately: layer clothing for warmth
rooms are often damp. and wear insulated gloves to protect your hands.
stir plates (not hot plates) in the cold room
• Should be plugged directly into an outlet. • NEVER store flammables, dry ice, or hazardous
Do not use extension cords. liquid chemicals in the cold room.
• Should be allowed to equilibrate prior to • It is critical to keep doors closed to maximize
use after removal from a cold room, as ventilation efficiency.
condensation can occur. • Avoid mold contamination by keeping cardboard
or other paper products out of the cold room.
• Include emergency contact information posted
outside of the cold room.
Prevent Asphyxiation
No fresh air circulates in a
cold room!
NO hazardous chemicals,
pressurized gases or dry ice
Avoid Mold Contamination

Preserve your health and
scientific data!
NO Hazardous
NO Pressurized Gases NO Dry Ice
NO food, drink, cardboard or Chemicals
other paper products
Maintain Equipment
Integrity
Prevent rust and corrosion!
AVOID moisture build-up;
keep the door closed
AVOID cluttering and trash

accumulation NO Cardboard or DO Clean up spills &
NO Food or Drink Other Paper Goods dispose of trash
CLEAN UP all spilled materials
immediately
1§11 Ventilation
Ell Personal Protective Equipment (PPE)
PPE General Guidelines
Using personal protective equipment is often Factors to consider are the type of potential
essential, but it is generally the last line of chemical, length and route of exposure, and
defense after engineering controls, work type of contact {splash, mist, vapor, occasional
practices, and administrative controls. or continuous immersion). Contact a OOHS
safety specialist for assistance in selecting
Minimum protection appropriate gloves and respiratory protection.
All persons working in the lab should have The use of respiratory protection requires an
clothes that cover their legs and arms, and industrial hygiene hazard assessment and a
closed-toe shoes. When handling hazardous medical clearance by OMS, followed by a fit
chemicals, appropriate eye protection, test and training by the OOHS TAB.
appropriate gloves and laboratory coats Lab coats, gloves and eye protection provide
must be worn. This is the minimum protection
Chemical resistant gloves minimum safeguarding.
required and must be upgraded as appropriate Gloves must be selected on the basis of their
for the risk. chemical resistance to the material(s) being
handled, their suitability for the procedures
Additional PPE being conducted, their resistance to wear as
Additional PPE must be worn if indicated by well as temperature extremes. Consult the
the potential exposure, such as: Glove Selection section for detailed guidance
• splash goggles for selecting chemical resistant gloves, or
contact your OOHS safety specialist.
• face shields
• chemical aprons riJJDlil Improper selection may result

• disposable coveralls in glove degradation, penetration of the
chemical through the glove, and ultimately
• chemical resistant gloves
exposure to the chemical.
• respiratory protection
• chemical resistant footwear
Wear add�nalPPEas needed.
Safety goggles with

side shields Lab coat Closed-toe shoes
Chemical
Chemical resistant Respiratory protection apron Wear respiratory protection as needed.
gloves
Contact OOHS for enrollment in the respiratory protection
program and fit-testing at [email protected].
Lab Coat Selection
Lab coat selection and use: • Most synthetic material resist ignition but • In most cases, lab coats will need to be
once ignited will melt and can cause severe supplemented with additional protective
• Fabric weight and weave affect how easily
localized burns. equipment.
material ignites and burns; select tight
weave, heavy weight, and tightly fitted • Blended fabrics often have a high rate of • Lab coats should extend to the knees
sleeves/cuffs for work with flammables burning combined with fabric melting. and be fully buttoned down with sleeves
and pyrophorics. rolled down.
• Reusable lab coats should be cleaned and
dried when contaminated, and replaced • Don't wear lab coats in public places,
• Launder weekly or earlier if contaminated;
when damaged. Do not clean at home. such as offices, lunchrooms or lounge
use commercial laundry only.
areas as they can transfer hazardous
• Use only fire-retardant lab coats with pyro
materials and contaminate these areas.
• Cuffed sleeves prevent exposure to wrists phoric or highly flammable chemicals.
and arms. • Tyvek® coveralls can be used over street
• Follow lab coat supplier's instructions for clothes for protection against particles.
• Closeable lapel is preferred for maximum laundering and replacement. However, they do not provide sufficient
coverage from hazards. protection against liquids.
• Consider heavy-duty materials such as Tychem®
for chemical contact. Tychem® is a Tyvek® • Cotton lab coats are preferred over
• Consider sturdy chemical-resistant aprons
fabric coated with polyethylene and provides polyester as they are more breathable
for high-hazard work such as with pyrophorics,
protection against minor chemical spills and and flame resistant.
flammables, corrosives, etc.
splashes.
Lab Coat Type Fabric Type Protective Use Improper Use
Standard 100% cotton (preferred Minimal risk chemicals Moderate to high

for maximum protection) with low risk of exposure to volume use of chemicals,
or cotton/polyester blend fire. Radioactive materials, flammables, pyrophorics
fabric (burns more readily cryogenics, and lasers when the risk of expo-
than 100% cotton) (general benchwork) sure to fire is high
Fluid/Splash and Tear Polyethylene-coated Human and animal blood Use with flammables
Resistant polypropylene and body fluids, BSL2/3 and pyrophorics
(disposable) and BSL3 and bloodborne
pathogens
Chemical resistant Polyethylene-coated high Select chemical exposure Use with flammables
apron and sleeves density polyethylene, and pyrophorics
neoprene, vinyl, pvc,
polyester or polyethylene
-coated polypropylene
Nomex Flame resistant fabric Flame or pyrophoric Non-flammable or

(Flame Resistant) liquids and solids. Wear pyrophoric liquids and
with a chemical apron solids
when working with non-
polar organic solvents
such as acetone,
dichloromethane,
diethylamaine, ethyl
acetate, n-hexane,
tetrahydrofuran, toulene
Spunbonded olefin Tear resistant fabric/high Biological material or Best at protecting

such as Tyvek® -density polyethylene particulate and clean against dry substances
fibers rooms but minimal protection
against liquids/splashes

Glove Selection
Glove selection and use: Factors to consider when choosing Consider using:
a chemical resistant glove:
• All gloves are permeable and the changes • Knit glove liners to absorb perspiration.
are not always apparent. • frequency and duration of chemical
contact • Extended cuffs to protect wrists and
• Visible degradation of gloves can include forearms.
swelling, softening, hardening and • nature of contact (immersion and/or
• Ultra-thin gloves to provide greater tactile
discoloration. https//www.aaesi.com/wp splash)
sensitivity.
contentjplugins/pdf-poster/pdfjs/web/
viewer.html?file=https//www.aaesi.com/ • concentration of chemical • Textured finishes to provide better grip.
wp-contentjuploads/2020/07 /Anse11_8th
EditionChemicalResistanceGuide.pdf&down • chemical compatibility
load=true&print=&openfile=false.
• temperature of chemical
• Different gloves are resistant to different
chemicals. • abrasion-resistance requirements
• Multiple gloves can be worn together • puncture, snag, tear, and cut-resistance
for greater protection (use smallest size that
will provide adequate dexterity and comfort). • length of hand and arm to be protected
• Reusable gloves can be used for • dexterity requirements

intermittent chemical work. They must
be properly rinsed and air dried. Always • grip requirements and conditions
inspect reusable gloves for integrity (e.g., wet or oily)
before use.
• thermal protection - to protect against
• Disposable gloves provide barrier protection heat and cold
for small amounts of chemicals. They should
be replaced immediately when • size
contaminated, and never be reused.
• comfort
• Glove liners may be reused, but must be
Always consult the manufacturer's glove
replaced when contaminated.
selection guidelines, and your DOHS safety
• Latex deteriorates quickly when exposed to specialist for help in selecting the appropriate
petroleum products. glove.
• Surgical latex gloves are thicker than latex

exam gloves.
Always consult the manufacturer's glove selection guidelines, and your OOHS safety specialist for help in selecting the appropriate glove. Consult the following resource
for glove permeability information: https://www.aaesi.com/ansell_8th-edition-chemical-resistance-guide/
Glove Type Uses Caution
Do NOT use for solvents and corrosives.

Disposable: vinyl, latex, nitrile Dry powders, aqueous solutions Disposable gloves must be replaced immediately
upon chemical contamination.
Reusable: Neoprene (Black) Corrosives, solvents and alcohols; Must be properly rinsed and dried after each use.
Resists oils and offers less fatigue
Reusable: Nitrile (Blue or Green) Organic solvents (non-halogenated); Must be properly rinsed and dried after each use.
Puncture and abrasion resistant
Reusable: Nomex® or Zetex® Temperature extremes Must be properly rinsed and dried after each use.
Reusable: Butyl Aldehydes, ketones and esters Must be properly rinsed and dried after each use.
Reusable: Viton® Chlorinated and aromatic solvents Must be properly rinsed and dried after each use.
Eye and Face Protection Selection
Eye and face protection selection and use:

• Eye protection is mandatory where there is potential for injury.
• Eye protection must be appropriate for the type of hazard

(e.g. chemical splash and vapors, impact hazards, lasers, ultra
violet light).
Safety Glasses/Spectacles: Designed to protect against impact

hazards. Additional PPE, such as face shields, are to be used
simultaneously when working with chemicals.
When working with chemical substances which may result in eye

contact in the form of splash, mists, vapors or fumes one of the
following should be used:
Safety Goggles: Protect the eyes, eye sockets, and facial area
surrounding the eyes from chemical hazards. They provide a seal
around the eyes preventing entry under or around goggles. Safety
goggles must be fitted to worker's face, poorly fitted goggles will
not offer necessary protection.
- Non-vented goggles protect eyes from vapors, mists, fumes

or other hazards. Eyes must be completely covered, but the
material hazard does not require covering all exposed skin.
- Vented goggles protect from moderated quantities of liquids

with no vapor or mist danger. Several types exist. For example,
the common, hardware-store goggle has holes drilled into the
plastic. This is unsuitable for laboratory work because liquids
can get through the holes. Vented laboratory goggles have a
series of buttons embedded into the plastic. These buttons
house a baffle plate that allows air to pass but present a
physical barrier to liquids.
Face Shields: Shield entire face from a range of hazards. Face

shields are secondary protectors to be used in addition to primary
protection such as safety glasses or goggles.
OSHA's Eye and Protection eTool can be used as an additional

resource: https:j /www.osha.gov/etools

Chemical Segregation and Storage Table
Chemical Segregation
Class of Chemicals Common Chemical Additional Concerns and Common Incompatible Possible Reaction if
Examples Storage Recommendations Chemical Types Mixed/Health Concerns
Corrosive Acids-Organic • Acetic acid • Store in ventilated corrosives • Flammable liquids . Heat
.
• Butyric acid cabinet on protected shelving • Flammable solids • Gas generation
• Trifluoroacetic acid using secondary containment, • Bases • Violent reaction
• Propionic acid keep away from incompatible • Oxidizers DO NOT POUR WATER INTO
.
chemicals • Inorganic acids ACID
• Formic acid
• Do not store under the sink • Causes skin burns
• Carbonic acid • Cyanides
• Do not store acids on metal Respiratory distress
• Benzoic acid • Sulfides
shelving • Use NIOSH approved
• Poisons/toxins gloves, eye protection,
• See compatibility chart for storage
face shield and apron
Corrosive Acids-Inorganic • Nitric acid • Store concentrated nitric acid • Flammable liquids • Heat
• Sulfuric acid (:2'.68%) and sulfuric acid (:2'.93%) in • Flammable solids • Gas generation
• Phosphoric acid a secondary container. Store in a • Bases • Violent reaction
• Hydrochloric acid corrosive cabinet labeled "Acid" or • Oxidizers • DO NOT POUR WATER INTO
on shelving using a secondary ACID
• Hydrofluoric acid • Organic acids
containment • Hydrofluoric acid can result
• Cyanides
• Do not store under the sink in skin irritation and causes
• Sulfides
• Do not store acids on metal burns
• Poisons/toxins • Respiratory distress
shelving
• Use NIOSH approved gloves,
• Hydrofluoric acid (HF) should only eye protection, face shield
be handled by trained personnel and apron
as it requires extra handling
precautions. Store in a secondary
container. HF is a bone decalcifier.
Corrosive Bases • Hydroxylamine • Store in separate cabinet • Acids • Heat

Organic/Caustic • Tetramethylethylamine preferably with ventilation, • Oxidizers • Gas generation
• Diamine corrosive cabinet or storage area • Flammable liquids/solids • Violent reaction
• Triethylamine with a spill tray, away from • Inorganic bases • Skin irritation and burn
potential water sources (DO NOT • Poisons/toxins • Respiratory distress
• Proplylenediamine
store under sink) • Amines are generally • Foul odor
• Cuprietylenediamine incompatible with • Use NIOSH approved
solution
isocyanates, halogenated gloves, eye protection,
• Dicyclohexylamine organics, peroxides, acidic face shield & apron
phenols, epoxides,
anhydrides, and halides
Corrosive Bases • Ammonium hydroxide • Store in separate cabinet • Acids • Heat

Inorganic/Caustics • Potassium hydroxide preferably with ventilation, • Oxidizers • Gas generation
• Sodium hydroxide corrosive cabinet or storage area • Flammable liquids • Violent reaction
• Calcium hydroxide with a spill tray, away from • Flammable solids • Skin burn and irritation
• Sodium hydroxide potential water sources (DO NOT • Organic bases • Respiratory distress
• Sodium hypochlorite store under the sink) • Poisons/toxins • Use NIOSH approved
solution (bleach) • Store solutions of inorganic gloves, eye protection,
• Magnesium hydroxide hydroxides in labeled polyethylene face shield and apron
containers
Examples Storage Recommendations Chemicals Types Mixed/Health Concerns
Flammable Solids • Paraformaldehyde • Keep in a dry, cool area away • Acids • Fire hazard
Phosphorus from oxidizers and corrosives • Bases • Violent reaction
• Magnesium • Follow specific safety procedures • Generates toxic fumes
• Oxidizers
• Sulfur • Conduct work on small scale if • Respiratory distress
possible • Poisons/toxins
• Potassium sulphide • Keep away from ignition & sparks
• Keep amounts on-hand to a • Ignites readily, burns fiercely
• Naphthalene
minimum
• Camphor • Use NIOSH approved gloves,
• Keep away from other flammables eye protection, face shield
and apron
Flammable Liquids • Ethanol • Flammable storage cabinet or • Oxidizers • Fire hazard

• Ethyl acetate refrigerator rated for flammable/ • Acids • Heat
• Methanol hazardous storage/explosion proof • Bases • Violent reaction
• Acetone • Peroxide-forming chemicals must • Reactives • Watch for vapor mist
• Benzene be dated upon delivery and opening • Poisons/toxins • Causes eye and skin irritation
• Xylene (two dates) ie., di-ethyl ether, • Keep away from ignition or
• Toluene tetrahydrofuran, furan, methyl sparks
• Diethyl ether butanol, methyl acetylene, • Use NIOSH approved gloves,
• Tetrahydrofuran heptanol, dioxanes, diglyme eye protection, face shield
• Acetonitrile and apron
• Please consult NIH Chemical
• Propanol Hygiene Plan Peroxide Formers
• Gasoline
Toxic • Chloroform • Store in a dark, dry, ventilated, cool • Flammable liquids • Generation of toxic and
• Cyanides area in an unbreakable chemically • Acids flammable gas
• Heavy metal resistant secondary container • Bases • Combustible
compounds (e.g. (polyethylene) • Reactives • Heat
Cadmium, Mercury, • Fire hazard
Osmium, Arsenic, • Store volatile toxic materials with • Oxidizers
• Explosion hazard
Barium) evaporation rate above 1.0 - (ether • Corrosives • Violent reaction
• Formamide =1.0) in flammable cabinet • Please consult Division • Chloroform explosively reacts
• Phenol • Store non-volatile liquid poisons in of Environmental with chemically-reactive metals
• Carbon tetracholride a refrigerator or cabinet; amounts Protection (DEP) for (e.g., aluminum or magnesium
assistance powder, sodium, and lithium),
• 2-Mercaptoethanol less than 1 liter can be stored in a
cabinet above bench level, ONLY if strong oxidizers, strong caustics
• Acrylamide (e.g., alkalis), and decomposes in
• Ethidium bromide the cabinet has sliding doors (not
sunlight
• Sodium azide solution swinging)
• Some toxins are mutagenic
• Sodium azide, must be kept and carcinogenic
refrigerated
• Review your SDS before
working with toxic material
eye protection, face shield
and apron
Explosives • Picric acid (dry) • Store in a secure location away • Please consult the SDS • Explosion hazard
• Ammonium nitrate from other chemicals; store in an and the DEP • Violent reaction
• Nitro urea area away from friction or shock • Heat
• Explosives must be
• Trinitroaniline • Store Picric acid in cool location or stored as "STAND • Shock sensitive
• Benzoyl peroxide (dry) in a hazard rated fridge to prevent ALONE." They must • Regular inspection may be
• Trinitrobenzene explosive crystallization never be stored with required, to check for
• Trinitrobenzoic acid any chemicals of any deposits or crystallization
• Trinitrotoluene • Storage regulations DO NOT apply kind • Use spark proof tools
• Urea nitrate to binary explosives until mixed.
Consult Explosive Expert of DEP • Use NIOSH approved gloves,
• Trinitrophenol eye protection, face shield
• Diazoisbutylnitrile and apron
• Sodium azide (solid)
Oxidizers • Peroxides • Store in secondary containment • Combustibles • Fire hazard

• Nitrates separately from combustibles and • Flammables • Gas generation
• Perchlorates flammable materials • Organic materials • Toxic gas
• Permanganates • May explosively decompose on • Reducing agents • Explosion hazard
• Sodium hypochlorite shock, friction, or concussion • Forms irritating toxic fumes
(solid) • May EXPLODE ON HEATING, to • Use NIOSH approved
• Potassium form irritating toxic fumes and gloves, eye protection, face
dichromate gases of Benzoic Acid and Carbon shield & apron
• Chlorates Monoxide. Its a strong oxidant and
• Chlorites reacts violently with combustible,
• Chromates organic and inorganic acids, and
• Bromates reducing materials, causing fire
• Superoxides and explosion hazard. Attacks
some forms of plastics, rubber or
coatings
Peroxide Formers • Acrylonitrile • Store in airtight bottles, away from • Always consult the • Explosion hazard
• lsopropyl alcohol light and heat in a dark, cool dry Safety Data Sheet • Violent reaction
• Ethers (e.g. diethyl area; avoid using containers with (SDS) and the Division • Shock sensitive
loose-fitting lids and ground glass of Environmental • Combustion
ether, isopropyl ether) Protection (DEP)
• Acetals and ketals, stoppers; crystallization, discoloration, (exothermic reaction)
and formation or deposition of • If an old or expired
especially cyclic
layers are signs a peroxide former container of a peroxide
ethers and those with
may have become shock sensitive; forming chemical or reactive
primary and/or
do not use or move such containers, is found, do not move it.
secondary alkyl groups
contact DEP Contact the DEP at
or aldehydes (e.g.
acetaldehyde, ben- • All bottles of peroxide-forming 301-496-4710 for
zaldehyde chemicals must have the received assistance in disposing of
date marked on the container; the container.
• Vinyl and vinylidene
compounds when the bottle is first opened, • Use proper PPE
the container must be marked
• Dienes • Use NIOSH approved gloves,
with the date opened
• tetrahydrofuran eye protection, face shield
dioxane & apron
• Butylated
hydroxytoluene (BHT)
Water Reactive • Sodium Metals • Store in a dry, cool area away from • Aqueous solutions • Heat evolution
• Lithium Metals potential spray from fire sprinklers
• Oxidizers • Violent reaction when
• Potassium Metals and other water sources (DO NOT
store under the sink) • Please consult the Safety mix with water
• Sodium Borohydride
• Label this area for water-reactive Data Sheet (SDS) and • Liberates hydrogen gas
• Alkali Metal Hydrides the Division of with water
storage
• Cesium Metal Environmental Protection
• Do not store with any other • Reacts violently with water
(DEP)
chemicals • Use NIOSH approved gloves,
eye protection, face shield
and apron
Flammable Compressed • Methane • Handle flammable compressed • Oxidizers • Fire hazard

Gases • Acetylene gases in a chemical fume hood • Toxic compressed gases • Explosion hazard
• Butane • Store in well-ventilated areas; • Use NIOSH approved
• Propane store away from oxidizers, open gloves, eye protection,
• Hydrogen flames, sparks, and other sources face shield and apron
• Silane of heat ignition; post NO SMOKING • Wear safety shoes
• Ethane signs around storage area(s) or
• Arsine entrance(s) to storage room(s);
• Germane flammable gases stored outdoors
where ambient temperatures
exceed 125 degrees F
(51.7 degrees C) shall be
protected from direct sunlight
• Must be secured in upright
position, bonded or chained
against the wall
• Use a spark proof wrench to

attach regulators and make
other connections; install a
flame/flash arrestor at the
regulator outlet flow valve
Oxidizing Compressed • Oxygen • Store oxidizers separately from • Flammable compressed • Fire hazard
Gases • Chlorine flammable gas containers or gases • Explosion hazard
• Fluorine combustible materials; • Toxic compressed gases • Use NIOSH approved
• Nitrogen oxides minimum separation gloves, eye
• Gas mixtures requirement from these protection and apron
containing oxygen materials is 20 ft. or a 5 ft. • Wear safety shoes
higher than noncombustible barrier with a
atmospheric fire resistance rating of at least
concentrations 30 minutes
(above 23%)
• Must be secured in upright
position, bonded or chained
against the wall
• Clean equipment used for
Oxygen and Nitrous Oxide with
Oxygen compatible materials
free from oils, greases and
other contaminants
• Fluorine shall be handled in
specially passivated containers
and associated equipment
Toxic Compressed • Carbon monoxide • Handle toxic compressed gases in • Flammable compressed • Release of toxic gas
Gases • Hydrogen dioxide a chemical fume hood gases
• Hydrogen sulfide is a
• Arsenic • Must be secured in upright • Oxidizing compressed colorless, flammable,
• Pentafluoride position, bonded or chained gases extremely hazardous gas
• Boron tribromide against the wall • Please consult the with a "rotten egg" smell;
• Bromine • Indoor storage or use of toxic specific SDS and DEP • Prolonged exposure may
• Chlorine compressed gases shall be cause nausea, tearing of
• Fluorine provided with a gas cabinet, the eyes, headaches or loss
• Chloropicrin exhausted enclosure, or gas room of sleep, or bronchial
• Cyanogen • Refer to the SOS information for constriction in some
additional guidance on the storage asthma patients
and compatibility requirements • Possible fatigue, loss of
• Contact OOHS to determine if a appetite, headache,
fail-safe valve and/or continuous irritability, poor memory,
monitoring for toxic gas may be dizziness and slight
required during use conjunctivitis
eye protection, face shield,
& apron
• Wear safety shoes
Carcinogens • Benzene • Label all containers as "Cancer • Please consult the specific • Please consult the
• Benzadine Suspect Agents" or the equivalent SOS and DEP specific SOS and DEP
• Methylene chloride • Store according to the hazardous • Use NIOSH approved gloves,
• Carbon tetrachloride nature of the chemical, using eye protection, face shield,
• Cadmium & appropriate security when and apron
compounds necessary
• Arsenic &
compounds
• Asbestos
• Alfatoxins
• Beryllium &
compounds
Teratogens • Tegretol • Label all containers as "Suspect • Aniline is incompatible • Please consult the
• Aminopterin Reproductive Hazard" or with nitric acid and specific SDS and DEP
• Chlorobiphenyls "Reproductive Effecter" hydrogen peroxide • Use NIOSH approved gloves,
• Coumarins • Store according to the hazardous • Please consult the eye protection, face shield,
• Tetracycline nature of the chemical, using specific SOS and DEP and apron
• Tapazole appropriate security when
• Propylthioracil (PTU) necessary
Flammable Aerosol Cans Pressurized Aerosol cans Content under pressure: • See incompatibles for • Read instructions and usage
containing flammable • Store at room temperatur; or flammable liquids as directed
liquid not limited to: store above 120°F • Review SOS prior to use
• Do not store with acids,
• Acetone oxidizers, toxic and reactive
• Do not use near heat, sparks and • Use NIOSH approve gloves or
• Thinner chemicals PPE. Rinse skin thoroughly
open flames
• Toluene with soap and water
• Always use secondary containers • Use secondary container
• Petroleum Distillates
when storing with other chemicals with flat surfaces for stability • Contact emergency services
• Butyl Cellusolve
for severe skin impact or
• Xylenes eye exposure
• Methanol
• Use fire extinguisher in case
of fire or dial 911
Non-Flammable Pressurized aerosol cans Content under pressure: • See incompatibles for • Read instructions and use
Corrosive-Toxic NOT containing flammable • Store at room temperature corrosive acid, base and as directed
Aerosol Cans liquid but not limited to • Do not use near heat, sparks and toxic items above
• Review SOS prior to use
corrosive or toxic carrier: open flames • Use secondary container • Use NIOSH approve gloves or
• Ammonia • Always use secondary containers with flat surface for PPE. Rinse skin thoroughly
• Sodium hydroxide when storing with other chemicals stability with soap and water
• Sodium hypochlorite
• Contact emergency services
• Amines
for severe skin impact or eye
exposure
• Use fire extinguisher in case
of fire or dial 911
1. Adapted from Prudent Practices in the La bora tory: Handling and Disposal of Chemica ls, Na tiona l Research Council, 2011.
2. Boston University Environmental Health and Safety. (2011) Chemical Segregation and Storage.
3. The University of Texas Health Science Center at Texas. (2022). Chemical Segregation & Incompatibilities Guidelines. Chemical Hygiene Plan.
Contact Useful Websites
Division of Occupational Health and Safety
Office of Research Services National Library of Medicine Gateway
National Institutes of Health https:j/www.nlm.nih.gov/
U.S. Department of Health and Human Services
Building 13, Room 3K04 13 South Drive, MSC 5760 Agency for Toxic Substances and Disease Registry
Bethesda, MD 20892-0003 https:j/www.atsdr.cdc.gov/
Telephone: (301) 496-2960
Fax: (301) 402-0313
https://ors.od.nih.gov/sr/dohs/Pages/default.aspx
PUB CHEM
https:j/pubchem.ncbi.nlm.nih.gov/
NIOSH Pocket Guide to Chemical Hazards

https://www.cdc.gov/niosh/npg/default.html
National Institutes of Health Resources OSHA Laboratory Standard - Safety & Health Topics
https://www.osha.gov/laboratories/standards
Division of Environmental Protection (DEP)
https:j/orf.od.nih.gov/AboutORF/Organization/Pages/dep_
info.aspx
301-496-3537 OSHA Hazardous & Toxic Substances
■
https://www.osha.gov/chemical-hazards/standards
Chemical Waste
https:j/orf.od.nih.gov/EnvironmentalProtection/
WasteDisposaljPages/chemicalwaste.aspx Chemical Safety Board - Video on Lab Accidents,
. . Pick-up: 301-496-4710
Assistance: 301-496-7990 "Experimenting with Danger"
[!I •• .•
https://www.csb.gov/videos/experimenting-with-danger/
Radioactive Waste
https://orf.od.n ih .gov/EnvironmentalProtection/
Argonne National Labs
WasteDisposa1/Pages/radwaste.aspx
https://www.anl.gov/
Pick-up: 301-496-4551
Assistance: 301-496-5774
Division of Fire Rescue Services (DFRS)

DrugBank
� https:j/ors.od.nih.gov/ser/dfrs/Pages/default.aspx
Pick-up: 301-496-2372 https://www.drugbank.com/
� Email: [email protected]
Sigma-Aldrich
https://www.sigmaaIdrich.com/US/en
Physicians' Desk Reference (PDR) Health

0 https:j/www.pdr.net/
BE CAREFUL Chemical Compatibility Chart
BE AWARE https://www.ehs.harvard.edu/sites/ehs.harvard.edu/
files/chemical_waste_chemical_compatibility_chart.pdf
BE SAFE
NIH personal Property Management Guide
https://policymanual.nih.gov/26101-25-2/
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EID Hazard Identification

Globally Harmonized System GHS Pictograms:

hazards, as well as classification of mixtures. GAS CYLINDER CORROSION EXPLODING BOMB

Labels: Chemical manufacturers and importers

Safety Data Sheets (SDS): Previously called,

Signal Word: There are two signal words in the

Information and Training: While the GHS does not

EID Hazard Identification

. ..... .... ... • .. . ...... . ... ..

Hierarchy of Safety Controls

Administrative Change the

Protect the workers with

■I Hazard Control/Hazardous Spill Procedure

Hazard Control/Hazardous Spill Procedure

• Do not store hazardous chemicals under a sink. Chemical Transport

!RF-Frederick: Contact DEP at (240) 236-9575.

Baltimore: Contact DEP at (301) 346-6287 or

Baltimore: (301) 346-6287

Pleaserefer to the NIH Waste Disposal Guidefor

Chemical Waste Tag and examples of correct disposal techniques

employees should conduct a Chemical Hazard

Suggested Storage Time Limits for Common Peroxide Forming Compounds

CLASS A: MOST DANGEROUS CLASS B: DANGEROUS CLASS C: DANGEROUS

lsopropyl ether Acetal Dioxane Acrylic acid

Safe Management of Particularly Hazardous Substances in NIH Laboratories

Toxic Chemical Reduction Initiative

Hazards associated with compressed gases Handling

Where more than one type of gas is in use, label

Requirements Manual for Biomedical

Laboratories and Animal Research Facilities

rll Special Hazards

Chemical Fume Hoods and Other Local Exhaust Ventilation

The baffles direct the air being exhausted, -s

blocks the exhaust path.

Avoid Mold Contamination

AVOID cluttering and trash

• chemical aprons riJJDlil Improper selection may result

• chemical resistant footwear

Wear add�nalPPEas needed.

Safety goggles with

Lab Coat Type Fabric Type Protective Use Improper Use

Standard 100% cotton (preferred Minimal risk chemicals Moderate to high

Nomex Flame resistant fabric Flame or pyrophoric Non-flammable or

Spunbonded olefin Tear resistant fabric/high Biological material or Best at protecting

Ell Personal Protective Equipment (PPE)

• Reusable gloves can be used for • dexterity requirements

• Surgical latex gloves are thicker than latex

Glove Type Uses Caution

Do NOT use for solvents and corrosives.

Eye and face protection selection and use:

• Eye protection must be appropriate for the type of hazard

Safety Glasses/Spectacles: Designed to protect against impact

When working with chemical substances which may result in eye

- Non-vented goggles protect eyes from vapors, mists, fumes

- Vented goggles protect from moderated quantities of liquids

Face Shields: Shield entire face from a range of hazards. Face

OSHA's Eye and Protection eTool can be used as an additional

Ell Personal Protective Equipment (PPE)

Corrosive Bases­ • Hydroxylamine • Store in separate cabinet • Acids • Heat

Corrosive Bases­ • Ammonium hydroxide • Store in separate cabinet • Acids • Heat

Corrosive Bases • Hydroxylamine • Store in separate cabinet • Acids • Heat

Corrosive Bases • Ammonium hydroxide • Store in separate cabinet • Acids • Heat