Product Sheet - Methylocystis Sp.

Methylocystis sp.
Product Sheet
49242™
Description
Strain designation: Rockwell
Deposited As: Methylosinus trichosporium (ex Whittenbury et al.) Bowman et al.
Type strain: No
Storage Conditions
Product format: Freeze-dried
Storage conditions: 2°C to 8°C
Intended Use
This product is intended for laboratory research use only. It is not intended for any
animal or human therapeutic use, any human or animal consumption, or any
diagnostic use.
BSL 1
ATCC determines the biosafety level of a material based on our risk assessment as
guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories
(BMBL), U.S. Department of Health and Human Services. It is your responsibility to
understand the hazards associated with the material per your organization’s policies
and procedures as well as any other applicable regulations as enforced by your local
or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always

used when handling vials. For cultures that require storage in liquid nitrogen, it is
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Methylocystis sp.
Product Sheet
49242
important to note that some vials may leak when submersed in liquid nitrogen and
will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid
nitrogen back to its gas phase may result in the vial exploding or blowing off its cap
with dangerous force creating flying debris. Unless necessary, ATCC recommends
that these cultures be stored in the vapor phase of liquid nitrogen rather than
submersed in liquid nitrogen.
Certificate of Analysis
For batch-specific test results, refer to the applicable certificate of analysis that can
be found at www.atcc.org.
Growth Conditions
Medium:
ATCC Medium 1683: Ammonium mineral salts medium without methanol
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature: 25°C
Atmosphere: 50% CH4, 50% Air
Incubation: Shake culture while incubating
Handling Procedures
1. Open vial according to enclosed instructions.
2. Using a single tube of broth, aseptically transfer 0.5 mL to the vial and
rehydrate the entire pellet.
3. Aseptically transfer this aliquot back to the broth tube and mix well. When
using Medium #1683 use a gas mixture of 50% methane and 50% air.
4. Transfer 0.5 mL to two or three additional broths of #1683
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Methylocystis sp.
Product Sheet
49242
5. Incubate the culture at 25°C while shaking for 3-5 days. When using Medium
#1683 the culture needs to be fed a mixture of 50% methane and 50% air
every 48-72 hours for best results.
Notes
Additional information on this culture is available on the ATCC® web site at
www.atcc.org.
Material Citation
If use of this material results in a scientific publication, please cite the material in the
following manner: Methylocystis sp. (ATCC 49242)
References
References and other information relating to this material are available at
www.atcc.org.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30
days from the date of shipment, provided that the customer has stored and handled
the product according to the information included on the product information sheet,
website, and Certificate of Analysis. For living cultures, ATCC lists the media
formulation and reagents that have been found to be effective for the product.
While other unspecified media and reagents may also produce satisfactory results, a
change in the ATCC and/or depositor-recommended protocols may affect the
recovery, growth, and/or function of the product. If an alternative medium
formulation or reagent is used, the ATCC warranty for viability is no longer valid.
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Methylocystis sp.
Product Sheet
49242
Except as expressly set forth herein, no other warranties of any kind are provided,
express or implied, including, but not limited to, any implied warranties of
merchantability, fitness for a particular purpose, manufacture according to cGMP
standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any
animal or human therapeutic use, any human or animal consumption, or any
diagnostic use. Any proposed commercial use is prohibited without a license from
ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information
on this product sheet, ATCC makes no warranties or representations as to its
accuracy. Citations from scientific literature and patents are provided for
informational purposes only. ATCC does not warrant that such information has been
confirmed to be accurate or complete and the customer bears the sole responsibility
of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and
assumes all risk and responsibility in connection with the receipt, handling, storage,
disposal, and use of the ATCC product including without limitation taking all
appropriate safety and handling precautions to minimize health or environmental
risk. As a condition of receiving the material, the customer agrees that any activity
undertaken with the ATCC product and any progeny or modifications will be
conducted in compliance with all applicable laws, regulations, and guidelines. This
product is provided 'AS IS' with no representations or warranties whatsoever except
as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries,
directors, officers, agents, employees, assigns, successors, and affiliates be liable for
indirect, special, incidental, or consequential damages of any kind in connection with
or arising out of the customer's use of the product. While reasonable effort is made
to ensure authenticity and reliability of materials on deposit, ATCC is not liable for
damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the
use of this product. The MTA is available at www.atcc.org.
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Methylocystis sp.
Product Sheet
49242
Copyright and Trademark Information

© ATCC 2023. All rights reserved.
ATCC is a registered trademark of the American Type Culture Collection.
Revision
This information on this document was last updated on 2022-10-22
Contact Information
ATCC
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Manassas, VA 20110-2209
USA
US telephone: 800-638-6597
Worldwide telephone: +1-703-365-2700
Email: [email protected] or contact your local distributor
www.atcc.org
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