Chapter 3

HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2

3.1 Lungs as Purification System:

Figure: Representing the oxygen-carbon dioxide exchange in the alveoli and capillary
Lungs as Purifier
The lung purifies air by removing harmful substances and adding oxygen to the
bloodstream. The process of purifying air in the lungs can be described as follows:
 Filtration: The nose and mouth serve as a first line of defense against harmful substances
in the air, such as dust, dirt, and bacteria. The tiny hairs in the nose, called cilia, and the

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 mucus produced by the respiratory system trap these substances and prevent them from
entering the lungs.
 Moisturization: The air is also humidified as it passes over the moist lining of the respiratory
tract, which helps to keep the airways moist and prevent them from drying out.
 Gas Exchange: Once the air reaches the alveoli, the gas exchange process occurs, where
oxygen diffuses across the thin alveolar and capillary walls into the bloodstream, and carbon
dioxide diffuses in the opposite direction, from the bloodstream into the alveoli to be
exhaled. This process ensures that the bloodstream is supplied with fresh, oxygen-rich air,
while waste carbon dioxide is removed from the body.
Overall, the lung serves as a vital purification system, filtering out harmful substances,
adding oxygen to the bloodstream, and removing waste carbon dioxide. It plays a critical role in
maintaining the body's homeostasis and supporting life.

3.1.1 Architecture of Lungs as Purification System

Figure: Representing structure of lung

The architecture of the lung is designed to maximize surface area for efficient gas exchange.
The lung is divided into several parts, including the trachea, bronchi, bronchioles, and alveoli.
 Trachea: The trachea is the main airway that leads from the larynx (voice box) to the lungs.
It is lined with cilia and mucus-secreting glands that help to filter out harmful substances
and trap them in the mucus.

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  Bronchi: The trachea branches into two main bronchi, one for each lung. The bronchi are
larger airways that continue to branch into smaller airways called bronchioles.
 Bronchioles: The bronchioles are smaller airways that eventually lead to the alveoli. They
are surrounded by tiny air sacs called alveoli, which are the sites of gas exchange.
 Alveoli: The alveoli are tiny air sacs that are lined with a network of capillaries. This close
proximity of the alveoli and capillaries allows for efficient diffusion of oxygen and carbon
dioxide between the air in the alveoli and the bloodstream.
Overall, the architecture of the lung is designed to provide a large surface area for gas
exchange, while filtering out harmful substances and humidifying the air. The close proximity of
the alveoli and capillaries, along with the moist lining of the respiratory tract, ensures that the air
is properly purified and the bloodstream is supplied with fresh, oxygen-rich air.

3.1.2 Gas Exchange Mechanism of Lung

The gas exchange mechanism in the lung involves the transfer of oxygen from the air in
the alveoli to the bloodstream, and the transfer of carbon dioxide from the bloodstream to the air
in the alveoli. This process is known as diffusion and occurs due to differences in partial pressures
of oxygen and carbon dioxide.
 Oxygen Diffusion: The partial pressure of oxygen in the air in the alveoli is higher than the
partial pressure of oxygen in the bloodstream. This difference creates a gradient that causes
oxygen to diffuse from the alveoli into the bloodstream, where it binds to hemoglobin in red
blood cells to form oxyhemoglobin.
 Carbon Dioxide Diffusion: The partial pressure of carbon dioxide in the bloodstream is
higher than the partial pressure of carbon dioxide in the air in the alveoli. This difference
creates a gradient that causes carbon dioxide to diffuse from the bloodstream into the alveoli,
where it is exhaled.

3.1.3 Spirometry
Spirometry is a diagnostic test that measures the function of the lungs by measuring the
amount and flow rate of air that can be exhaled. The test is commonly used to diagnose lung
conditions such as asthma, chronic obstructive pulmonary disease (COPD), and interstitial lung
disease.
Principle: The principle behind spirometry is to measure the volume of air that can be
exhaled from the lungs in a given time period. By measuring the volume of air exhaled, spirometry
can provide information about the functioning of the lungs and the ability of the lungs to move air
in and out.
Working: Spirometry is performed using a spirometer, a device that consists of a
mouthpiece, a flow sensor, and a volume sensor. The patient is asked to exhale as much air as
possible into the spirometer, and the spirometer measures the volume and flow rate of the exhaled
air. The volume of air exhaled is displayed on a graph called a flow-volume loop, which provides
information about the lung function.

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 Figure: Image of a spirometer
Interpretation of Results
The results of spirometry can be used to determine if the lungs are functioning normally and
to diagnose lung conditions. For example, a decrease in the volume of air exhaled or a decrease in
the flow rate of the exhaled air can indicate a restriction in the airways, which can bea sign of a
lung condition such as asthma or COPD.

3.1.4 Abnormal Lung Physiology - COPD

Figure: Representing the causes of COPD

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 Abnormal lung physiology refers to any deviation from the normal functioning of the
respiratory system. This can be caused by a variety of factors, including diseases, injuries, or genetic
conditions. Some common examples of abnormal lung physiology include:
 Asthma: A chronic inflammatory disease that causes the airways to narrow, making it
difficult to breathe.
 Chronic obstructive pulmonary disease (COPD): A progressive lung disease that makes it
hard to breathe and can include conditions such as emphysema and chronic bronchitis.
 Pulmonary fibrosis: A disease in which scar tissue builds up in the lungs, making it
difficult to breathe and reducing lung function.
 Pneumonia: An infection in the lungs that can cause inflammation and fluid buildup in
the air sacs.
 Pulmonary embolism: A blockage in one of the pulmonary arteries, usually by a blood
clot, which can cause lung damage and reduce oxygen flow to the body.
 Lung cancer: A type of cancer that originates in the lung and can impair lung function by
interfering with normal air flow and oxygen exchange.
Treatment for abnormal lung physiology depends on the underlying cause and may include
medications, lifestyle changes, or surgery.
It's important to seek prompt medical attention if you experience symptoms such as
shortness of breath, wheezing, or chest pain, as these can be indicative of a serious lung problem.
Chronic Obstructive Pulmonary Disease
Chronic Obstructive Pulmonary Disease (COPD) is a group of progressive lung diseases that
cause breathing difficulties. It's characterized by persistent airflow limitation that is not fully
reversible. The two main forms of COPD are chronic bronchitis and emphysema.
In COPD, the airways and small air sacs (alveoli) in the lungs become damaged or blocked,
leading to difficulty in exhaling air. This results in a decrease in lung function, leading to shortness
of breath, wheezing, and coughing. Over time, these symptoms can get worse and limit a person's
ability to perform everyday activities.
The primary cause of COPD is long-term exposure to irritants such as tobacco smoke, air
pollution, and dust. Other risk factors include a history of frequent lung infections, a family history
of lung disease, and exposure to second-hand smoke.
There is no cure for COPD, but treatment can help manage the symptoms and slow the
progression of the disease. Treatment options include medication, such as bronchodilators and
steroids, oxygen therapy, and lung rehabilitation. In severe cases, surgery may also be an option.
In addition, quitting smoking and avoiding exposure to irritants is crucial in managing COPD.

3.1.5 Ventilators
Ventilators are medical devices used to assist or control breathing in individuals who are
unable to breathe adequately on their own. They are commonly used in the treatment of acute
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respiratory failure, which can occur as a result of a variety of conditions such as pneumonia,
severe asthma, and chronic obstructive pulmonary disease (COPD).

Figure: Representing a ventilator machine

There are several different types of ventilators, including volume-controlled ventilators,
pressure-controlled ventilators, and bilevel positive airway pressure (BiPAP) devices. The type of
ventilator used depends on the patient's individual needs and the type of respiratory failure being
treated.
Ventilators work by delivering pressurized air or oxygen into the lungs through a breathing
tube or mask. The pressure can be adjusted to match the patient's needs and to help maintain
adequate oxygen levels in the blood.
While ventilators can be lifesaving for individuals with acute respiratory failure, they also
come with potential risks and complications. For example, prolonged use of a ventilator can increase
the risk of ventilator-associated pneumonia, and patients may experience discomfort or pain from
the breathing tube.
The use of ventilators is carefully monitored and managed by healthcare professionals to
ensure that the patient receives the appropriate level of support while minimizing potential risks and
complications.

3.1.6 Heart-Lung Machine

A heart-lung machine, also known as a cardiopulmonary bypass machine, is a device used
in cardiovascular surgery to temporarily take over the functions of the heart and lungs. The heart-
lung machine is used during open-heart surgery, such as coronary artery bypass graft (CABG)
surgery and valve replacement surgery, to support the patient's circulatory and respiratory functions
while the heart is stopped.
The heart-lung machine works by circulating blood outside of the body through a seriesof
tubes and pumps. Blood is taken from the body, oxygenated, and then returned to the body. This
allows the heart to be stopped during the surgery without causing any harm to the patient.

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 The use of a heart-lung machine during surgery carries some risks, including the potential
for blood clots, bleeding, and infections. Additionally, there may be some long-term effects on the
body, such as cognitive decline, that are not yet fully understood. However, the use of a heart-lung
machine has revolutionized the field of cardiovascular surgery, allowing for more complex
procedures to be performed and greatly improving patient outcomes.

Figure: Representing a heart-lung machine

3.1.7 Artificial Lungs

Artificial lungs are devices designed to mimic the function of the natural respiratory system.
They are used to support patients with acute respiratory distress syndrome (ARDS) or acute lung
injury (ALI) and to help the patient's own lungs recover and heal.
Types
There are two main types of artificial lungs: membrane oxygenators and extracorporeal lung
assist devices.
Membrane Oxygenators: These are devices that use a semipermeable membrane to transfer
oxygen and carbon dioxide between the blood and the air. The blood is pumped through the
membrane, where it comes into contact with air, allowing for the exchange of gases.

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 Figure: Representing a membrane oxygenator
Extracorporeal Lung Assist Devices: These devices work by removing carbon dioxide from
the blood and adding oxygen, allowing the patient's natural lungs to rest and heal. One example of
an extracorporeal lung assist device is the extracorporeal membrane oxygenation (ECMO) machine,
which is used to treat patients with severe respiratory failure. ECMO works by removing carbon
dioxide from the blood and adding oxygen, and it can be used as a bridge to recovery or as a bridge
to lung transplantation.

Figure: Representing veno-venous and veno-arterial extracorporeal membrane oxygenation

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Chapter 3
HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2

3.2 Kidney as a Filtration System:

Figure: Anatomy of kidney

The kidney is a complex organ that acts as a filtration system for the body. It removes waste
and excess fluid from the bloodstream and maintains a delicate balance of electrolytes, hormones,
and other substances that are critical for the body's normal functioning.
The kidney also plays an important role in regulating blood pressure by secreting the
hormone renin, which helps control the balance of fluid and electrolytes in the body. It also
regulates red blood cell production and the levels of various minerals in the blood, such as calcium
and phosphorus.
Without the kidney, waste and excess fluid would accumulate in the body, leading to serious
health problems.

3.2.1 Architecture of Kidney

The kidney is composed of functional units called nephrons, which are the basic structural
and functional units of the kidney. Each kidney contains approximately one million nephrons, and
each nephron performs the functions of filtration, reabsorption, and secretion.

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 Figure: Representing kidney and nephron

Figure: Representing the parts of nephron

The nephron is comprised of several key structures:
 Bowman's capsule: This is a cup-shaped structure that surrounds the glomerulus and
filters waste and excess fluid from the bloodstream into the renal tubule.
 Glomerulus: A network of tiny blood vessels within the Bowman's capsule that filters
waste and excess fluid from the bloodstream.
 Proximal convoluted tubule: A segment of the renal tubule that reabsorbs important
substances, such as glucose, amino acids, and electrolytes, back into the bloodstream.
 Loop of Henle: A U-shaped segment of the renal tubule that is critical for the
reabsorption of ions and water.
 Distal convoluted tubule: A segment of the renal tubule that regulates the levels of

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 electrolytes and other important substances in the bloodstream.
 Collecting duct: A series of ducts that collect the filtrate from the renal tubules and transport
it to the renal pelvis, where it drains into the ureter and eventually into the bladder.
The nephrons are surrounded by a network of blood vessels, including the afferent arteriole
and the efferent arteriole, which bring blood into and out of the glomerulus, respectively. The
filtrate produced by the nephron passes through the renal tubules, where it is modified by
reabsorption and secretion, before being eliminated from the body as urine.

3.2.2 Mechanism of Filtration – Urine Formation

Figure: Schematic of mechanism of filtration in human kidney

The mechanism of filtration in the kidneys is a complex process that involves multiple steps
to remove waste and excess fluids from the bloodstream. The following is a summary ofthe
steps involved in the filtration process:
 Blood enters the kidney through the renal arteries and flows into tiny filtering units called
glomeruli.
 At the glomerulus, the pressure in the blood vessels causes a portion of the plasma and
dissolved substances to filter out and enter a structure called Bowman's capsule.
 In Bowman's capsule, the filtrate is then transferred into the renal tubules, which are the
main filtering units of the kidneys.
 In the renal tubules, the filtrate passes through a series of specialized cells, such as proximal
tubular cells and distal tubular cells, which reabsorb important substances such as glucose,
amino acids, and electrolytes back into the bloodstream.
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  At the same time, the renal tubules secrete waste products, such as urea and creatinine,back
into the filtrate.
 Finally, the filtered fluid, now known as urine, is transported through the renal pelvis and
ureters to the bladder, where it is eventually eliminated from the body.
This process of filtration, reabsorption, and secretion helps to maintain the proper balance
of fluids and electrolytes in the body, as well as to remove waste and excess substances.

3.2.3 Chronic Kidney Disease (CKD)

CKD stands for Chronic Kidney Disease. It is a long-term condition in which the kidneys
gradually become less able to function properly. It can be caused by a variety of factors, including
diabetes, high blood pressure, and other health problems that damage the kidneys.
Symptoms of CKD include fatigue, swelling in the legs and feet, trouble sleeping, and
difficulty concentrating. As the disease progresses, it can lead to more serious complications, such
as anemia, nerve damage, and an increased risk of heart disease and stroke.
Treatment for CKD may include lifestyle changes, such as eating a healthy diet and
exercising regularly, as well as medications to manage symptoms and underlying health conditions.
In severe cases, kidney transplant or dialysis may be necessary.
It is important for individuals with risk factors for CKD to get regular check-ups and to talk
to their doctor about how to best manage their condition.

3.2.4 Dialysis Systems

Dialysis is a medical treatment that helps to filter waste and excess fluids from the blood
when the kidneys are unable to function properly. There are two main types of dialysis systems:
hemodialysis and peritoneal dialysis.
Hemodialysis is a procedure that uses a machine to clean the blood. During hemodialysis,
blood is removed from the body, passed through a dialysis machine that filters out waste and excess
fluids, and then returned to the body. Hemodialysis typically takes place in a hospital or dialysis
center, and is typically performed three times a week for three to four hours at a time.

Figure: Representing a Hemodialysis

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 Peritoneal dialysis is a type of dialysis that uses the lining of the abdomen, called the
peritoneum, to filter waste and excess fluids from the blood. A sterile solution is introduced into the
abdomen, where it absorbs waste and excess fluids, and is then drained and replaced with fresh
solution. Peritoneal dialysis can be performed at home and allows for more flexibility in scheduling.
Figure: Representing a Peritoneal dialysis

Both hemodialysis and peritoneal dialysis can effectively treat the symptoms of kidney
failure, but each has its own advantages and disadvantages. The choice of dialysis system depends
on various factors such as the individual's overall health, lifestyle, and personal preferences.

3.2.5 Artificial Kidney

While much progress has been made in developing an artificial kidney, it is still in the
experimental stage and is not yet widely available. Further research and development is needed to
improve the efficiency and safety of artificial kidney devices, and to ensure that they can be widely
adopted as a treatment for chronic kidney disease.

a)

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 b)
Figure: a) Schematic representation b) a prototype of artificial kidney
An artificial kidney is a device that is being developed to mimic the functions of the human
kidney. The goal of an artificial kidney is to provide a more effective and efficient meansof treating
patients with chronic kidney disease, who currently rely on dialysis or kidney transplantation.
There are currently two main approaches to developing an artificial kidney: a biological
approach and a technological approach.
The biological approach involves using living cells, such as kidney cells or stem cells, to
create a functional, implantable artificial kidney.
The technological approach involves using synthetic materials, such as silicon or polymer,
to create a dialysis device that can filter the blood and remove waste and excess fluids.
It's important to note that while the development of an artificial kidney holds great promise,
it is not a cure for chronic kidney disease and patients with kidney failure will still need dialysis or
kidney transplantation in the meantime.

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Chapter 3
HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2

3.3 Muscular Systems as Scaffolds:

The use of muscular systems as scaffolds in regenerative medicine is an area of active research and
development. Muscles have the potential to be used as scaffolds for the regeneration of tissues
due to their inherent mechanical properties and ability to support cell growth and tissue formation.
One example of using muscular systems as scaffolds is in the treatment of damaged or
diseased heart tissue. Researchers have developed methods for using muscle cells to create a
functional, three-dimensional scaffold that can support the growth of new heart tissue. In this
approach, muscle cells are harvested from the patient and then seeded onto a scaffold, such as a
hydrogel or artificial matrix. The scaffold provides a framework for the cells to grow and
differentiate into new heart tissue, which can help to repair the damaged or diseased tissue.
Another example is in the treatment of skeletal muscle injuries, such as those caused by
trauma or disease. In this case, muscle cells can be harvested and seeded onto a scaffold, whichcan
then be implanted into the damaged muscle to promote the growth of new, functional tissue.
While the use of muscular systems as scaffolds is still in the experimental stage, it holds
great promise for the treatment of a variety of conditions and represents an area of active research
and development in the field of regenerative medicine.

3.3.1 Architecture

Figure: The Three Connective Tissue Layers: Bundles of muscle fibers, called fascicles, are
covered by the perimysium. Muscle fibers are covered by the endomysium.
Inside each skeletal muscle, muscle fibers are organized into bundles, called fascicles,
surrounded by a middle layer of connective tissue called the perimysium. This fascicular
organization is common in muscles of the limbs; it allows the nervous system to trigger a specific
movement of a muscle by activating a subset of muscle fibers within a fascicle of the muscle. Inside
each fascicle, each muscle fiber is encased in a thin connective tissue layer of collagen and reticular
fibers called the endomysium. The endomysium surrounds the extracellular matrix of the cells
and plays a role in transferring force produced by the muscle fibers to the tendons.
Inside the muscle fibers, there are tiny structures called myofibrils. Myofibrils are made up
of smaller units called sarcomeres, which are responsible for muscle contraction.

Figure: Representing the sacromere

Sarcomeres contain thin (Actin) and thick filaments (Myosin) that work together to make
the muscle fibers contract. Each muscle fiber is surrounded by a protective layer called endomysium.
Multiple muscle fibers are grouped together into bundles called fascicles. Fascicles are surrounded
by another layer of connective tissue called perimysium.
All the fascicles together make up the entire muscle, which is surrounded by a layer called
epimysium. The muscle also has a special membrane called the sarcolemma, which protects the
muscle fiber. Inside the muscle fiber, there are small tunnels called T-tubules that help transmit
signals for muscle contraction. Muscles work through the coordination of motor units, which consist
of a motor neuron and the muscle fibers it controls. This architecture allows muscles to generate
force, move our bodies, and perform various activities.

3.3.2 Mechanisms
The mechanism of how the muscular system can be used as a scaffold in regenerative
medicine involves the use of muscle cells and a scaffold to support the growth and regeneration
of new tissue.
The method of growing muscle tissue using hydrogel or artificial scaffold is explained
below:
Figure: Representing the muscle tissue growth using hydrogel or artificial scaffold

Figure: Representing the formation of polymer based scaffold and cell culture
The basic steps in this process are as follows:
  Harvesting of muscle cells: Muscle cells are typically obtained from the patient and then
isolated and expanded in culture.
 Seeding onto scaffold: The muscle cells are then seeded onto a scaffold, such as a hydrogel
or artificial matrix. The scaffold provides a framework for the cells to grow and differentiate
into new tissue.
 Cell differentiation and tissue formation: Once the cells are seeded onto the scaffold, they
undergo differentiation, in which they change into specific cell types, such as muscle cells
or heart cells. The cells also begin to organize and form new tissue, such as heart tissue or
skeletal muscle tissue.
 Implantation into patient: The scaffold and cells are then implanted into the patient to
promote the growth of new, functional tissue.

3.3.3 Muscle Cells as Scaffold

Muscle cells can be used as a scaffold for tissue generation by removing the living cells from
the muscle tissue, leaving behind the structure known as the extracellular matrix (ECM). This
decellularized muscle scaffold provides a framework that can guide and support the growth of new
tissues.

Figure: Representing muscle scaffold for tissue growth

The Process
 Harvesting muscle tissue: A small sample of muscle tissue is taken, typically from a
donor or an animal model.
 Cell removal: The living cells within the muscle tissue are removed using a process
called decellularization. This involves treating the tissue with specific chemical solutions
 or enzymes that break down and wash away the cellular components, while preserving
the ECM.
 ECM scaffold: The remaining ECM, which forms the structure of the muscle, is now a
scaffold. It consists of proteins, such as collagen and elastin, and other molecules that
provide support and signals for tissue growth.
 Seeding cells: The decellularized muscle scaffold is then seeded with desired cells. These
can be stem cells or specialized cells relevant to the type of tissue being regenerated. The
cells are introduced onto the scaffold, allowing them to attach and populate the structure.
 Tissue growth: Over time, the seeded cells proliferate and differentiate, meaning they
multiply and transform into specific cell types required for the desired tissue. The ECM
scaffold guides the cells' growth, providing physical support, and biochemical cues to
influence their behavior.
 Tissue integration: As the cells continue to grow, they populate the scaffold and form new
tissue. The new tissue integrates with the surrounding native tissue, gradually replacing the
decellularized scaffold with functional, regenerated tissue.
By utilizing the decellularized muscle scaffold, the process of tissue generation takes
advantage of the existing three-dimensional architecture and mechanical properties of the muscle.
This approach has the potential to address challenges in tissue engineering, such as creating a
suitable environment for cell growth, promoting vascularization, and facilitating functional
integration of regenerated tissues.

3.3.4 Bioengineering Solutions for Muscular Dystrophy

Muscular dystrophy

Figure: Representing normal muscle and muscular dystrophy

Muscular dystrophy is a group of genetic disorders that result in progressive weakness and
degeneration of the skeletal muscles, which are responsible for movement. The disorders are caused
by mutations in genes that encode proteins needed for muscle function. The most common type
of muscular dystrophy is Duchenne muscular dystrophy, which typically affects young boys and
leads to severe disability by early adulthood. Other forms of the disease include Becker muscular
dystrophy, limb-girdle muscular dystrophy, and facioscapulohumeral dystrophy, among others.
 Duchenne muscular dystrophy (DMD) usually appears early in childhood between the ages
of 2 and 3. DMD primarily affects boys but can affect girls in rare cases. The primary symptom of
DMD is muscle weakness that begins in the muscles close to the body and later affects muscles in
the outer limbs.
Becker muscular dystrophy typically becomes apparent between the ages of 5 and 15. It
is similar to Duchenne MD, except that it progresses slower and symptoms begin to appear later.
Boys are primarily affected by Becker MD. Becker MD causes muscle loss that begins in the hips
and pelvic area, thighs, and shoulders.
The age of onset of limb-girdle muscular dystrophy is highly varied, ranging from early
childhood to later adulthood. The disease is characterized by muscle weakness and atrophy of the
muscles of the hip and shoulder areas (the limb girdles).
Facioscapulohumeral dystrophy (FSHD) typically appears before the age of 20, but can
appear later in adulthood or even in childhood in both males and females. FSHD affects the muscles
of the face, around the shoulder blades, and in the upper arms.
There is currently no cure for muscular dystrophy, but various treatments can help manage
symptoms and slow the progression of the disease. These may include physical therapy, assistive
devices, orthopedic surgery, and medication to manage muscle spasms and pain. In some cases,
genetic therapy and stem cell transplantation are also being explored as potential treatment options.
It's important for individuals with muscular dystrophy to work closely with a healthcare team
that includes specialists in neurology, rehabilitation medicine, and orthopedics, to develop a
comprehensive care plan that meets their specific needs.
Bioengineering solutions for muscular dystrophy
Bioengineering solutions for muscular dystrophy aim to improve the lives of individuals
affected by the disease by addressing the underlying genetic mutations and muscle weakness. Some
of the approaches being explored include:
 Gene therapy: This involves delivering a functional copy of the missing or mutated gene to the
affected muscle cells. The goal is to restore the production of the missing protein and improve
muscle function.
 Stem cell therapy: This involves using stem cells to replace the damaged muscle cells and
promote repair and regeneration of the muscle tissue. Stem cells can be taken from the patient's
own body (autologous stem cells) or from a donor (allogenic stem cells).
 Exoskeleton technology: This involves using wearable devices, such as robotic exoskeletons,
to support and enhance the movement of individuals with muscular dystrophy. The devices use
motors and sensors to mimic the movements of the wearer and help improve mobility.
 Tissue engineering: This involves using a combination of materials, such as scaffolds and
growth factors, to promote the growth and repair of muscle tissue. The goal is to create
functional muscle tissue that can replace the damaged tissue in individuals with muscular
dystrophy.
These approaches are still in the early stages of development, but hold promise for the future
treatment of muscular dystrophy. Clinical trials and further research are needed to determine the
safety and efficacy of these therapies.
3.3.5 Artificial Muscles
Artificial muscle refers to a type of technology that aims to mimic the properties and
functions of natural muscle. Artificial muscles can be made from various materials, including shape
memory alloys, electroactive polymers, and carbon nanotubes.
Shape Memory Alloys (SMAs) are materials with the ability to remember and recover their
original shape after being deformed. SMAs, like nickel-titanium (NiTi) alloys, are commonly used
in artificial muscle applications. When exposed to heat or an electric current, SMAs undergo a phase
transformation, enabling them to contract and generate force. This property makes them suitable
for mimicking muscle-like movements in devices such as prosthetics, robotics, and actuators. The
unique combination of shape memory and superelasticity in SMAs provides excellent mechanical
properties and durability for artificial muscle applications.
Electroactive Polymers (EAPs) are a class of materials that exhibit significant changes in
shape or size when subjected to an electric field. These polymers, such as polypyrrole and
polyacrylonitrile, have the ability to undergo large deformation and respond quickly to electrical
stimulation. EAPs are particularly advantageous for artificial muscle applications due to their
lightweight nature, flexibility, and biocompatibility. They can be designed to contract or expand in
response to electrical signals, enabling precise control and mimicry of muscle-like movements.
EAPs have promising potential in areas such as soft robotics, haptic devices, and biomedical
applications.
Carbon Nanotubes (CNTs) are cylindrical structures composed of carbon atoms, exhibiting
exceptional mechanical, electrical, and thermal properties. CNTs possess high tensile strength and
are highly conductive, making them suitable for artificial muscle development. By utilizing the
electromechanical properties of CNTs, they can act as actuators that contract or expand when
stimulated by an electric current. CNT-based artificial muscles offer advantages such as high
power-to-weight ratio, fast response times, and potential scalability. Research is underway to
optimize CNT-based artificial muscles for applications in robotics, aerospace, and
microelectromechanical systems (MEMS).
Artificial muscles have a number of potential applications, including:
 Robotics: Artificial muscles can be used to create more advanced and flexible robots that
can move and perform tasks more like humans.
 Prosthetics: Artificial muscles can be used to create more advanced prosthetic limbs that are
more responsive and capable of performing a wider range of movements.
 Biomedical devices: Artificial muscles can be used in various biomedical devices, such as
heart assist pumps and artificial hearts, to improve their performance and reliability.
 Textile and clothing applications: Artificial muscles can be integrated into textiles and clothing
to create smart garments that can change shape and adjust to the wearer's movements.
Artificial muscles have several advantages over traditional motors, including higher power-
to-weight ratios, faster response times, and greater flexibility. However, the technology is still in the
early stages of development and further research is needed to fully realize its potential and overcome
its limitations.
Chapter 3
HUMAN ORGAN SYSTEMS AND BIO-DESIGNS - 2

3.4 Skeletal Systems as Scaffolds:

3.4.1 Skeletal System
The skeletal system of human beings refers to the framework of bones, joints, and connective
tissues that provide structure, support, and protection to the body.
The key components and functions of the skeletal system are:
Bones: The human body consists of 206 bones that vary in size and shape. Bones are
composed of hard and dense connective tissue that provides strength and support. They serve asthe
anchor points for muscles, protect internal organs, and store minerals like calcium and phosphorus.
Cartilage: Cartilage is a flexible connective tissue found in certain joints and structures such
as the ears and nose. It acts as a cushion between bones, reducing friction and absorbing shock.
Ligaments: Ligaments are tough bands of fibrous tissue that connect bones to other bones
in joints, providing stability and preventing excessive movement.

Figure: Representing bone, cartilage, ligament

Tendons: Tendons are strong fibrous tissues that connect muscles to bones, enabling
movement by transmitting the force generated by muscles.
 Figure: Representing tendon
Axial Skeleton: The axial skeleton forms the central axis of the body and includes the skull,
vertebral column, and ribcage. The skull protects the brain, and the vertebral column (spine)
supports the body's weight and houses the spinal cord. The ribcage encloses and protects the heart,
lungs, and other thoracic organs.
Appendicular Skeleton: The appendicular skeleton comprises the bones of the limbs and the
shoulder and pelvic girdles. The upper limbs (arms) consist of the humerus (upper arm bone), radius
and ulna (forearm bones), and the hand bones. The lower limbs (legs) include the femur (thigh
bone), tibia and fibula (lower leg bones), and the foot bones. The shoulder and pelvic girdles attach
the limbs to the axial skeleton.

Figure: Representing axial and appendicular skeleton

Joints: Joints are the points where bones meet and allow for movement. There are different
types of joints, including hinge joints (e.g., elbow and knee) that enable bending and straightening,
ball-and-socket joints (e.g., hip and shoulder) that allow for a wide range of motion, and pivot
joints (e.g., between the atlas and axis vertebrae) that allow rotational movement.
 Figure: Representing various skeletal joints A) Ball and socket, B) Hinge, C) Pivot, D)
Ellipsoidal, E) Saddle, and F) Glider or planar
The skeletal system works in conjunction with muscles, tendons, and ligaments to allow for
movement, protect internal organs, support the body's weight, and provide a structural framework
for the body.

3.4.2 Skeletal System as Scaffold

The skeletal system can be used as a scaffold for tissue growth in certain applications.
Scaffold-based tissue engineering is a field that aims to create artificial scaffolds to support the
growth and regeneration of tissues and organs. In some cases, the natural structure of the skeletal
system can serve as a scaffold or template for tissue engineering purposes.
For example, bone tissue engineering often involves the use of scaffolds to facilitate the
repair and regeneration of bone defects or injuries. Synthetic or natural biomaterial scaffolds,
designed to mimic the properties of bone, can be used to fill the void left by a bone defect. The
scaffold provides a three-dimensional structure that supports the attachment, proliferation, and
differentiation of cells involved in bone regeneration. Over time, the scaffold can be replaced by
newly formed bone tissue, resulting in the restoration of bone structure and function.
In addition to bone tissue engineering, the skeletal system has also been explored as a
scaffold for other tissues. For instance, researchers have investigated using decellularized bone
or cartilage scaffolds as templates for the regeneration of other tissues like muscle, blood vessels,
or nerves. The existing extracellular matrix and structure of the skeletal system can provide a
framework for cells to populate and guide tissue growth.
However, it's important to note that using the skeletal system as a scaffold for tissue growth
requires careful consideration and modification to match the specific requirements of the target
tissue. Additional steps, such as surface modifications, incorporation of bioactive molecules, or
cell seeding, may be necessary to optimize the scaffold's effectiveness for promoting tissue
regeneration.
 Though the skeletal system has potential as a scaffold for tissue growth, successful
application requires further research, customization, and integration with tissue engineering
strategies specific to the desired tissue type.

3.4.3 Bioengineering Solutions for Osteoporosis

Osteoporosis
Osteoporosis is a condition that weakens the bones and makes them more likely to break
(fracture), especially the bones in the hip, spine, and wrist. It occurs when the body loses bone mass
and density more quickly than it can be replaced, leading to fragile bones that are prone to fracture.
Figure: Representing healthy bone and osteoporotic bone

Osteoporosis is a common condition, especially among older women, and it can increase the
risk of falls and fractures, which can result in significant pain and disability. Risk factors for
osteoporosis include being female, older age, having a family history of the condition, smoking,
drinking excessive amounts of alcohol, being thin or having a small body frame, and having alow
calcium intake.
Treatment for osteoporosis aims to slow down bone loss, prevent fractures, and treat
fractures if they occur. Some of the treatments include:
 Medications: Bisphosphonates, denosumab, and teriparatide are some of the medications
that can slow down bone loss and reduce the risk of fractures.
 Calcium and Vitamin D supplementation: Calcium and Vitamin D are essential for healthy
bones, and taking supplements can help maintain bone mass.
 Exercise: Weight-bearing and resistance exercises can help improve bone density and reduce
the risk of fractures.
 Lifestyle changes: Quitting smoking, reducing alcohol consumption, and eating a healthy diet
that includes enough calcium and Vitamin D can help maintain healthy bones.
It's important to work closely with a healthcare provider to develop a comprehensive
treatment plan for osteoporosis, as the right approach may vary depending on the individual's
specific needs and medical history.
Bioengineering solutions for osteoporosis
Bioengineering solutions for osteoporosis aim to improve bone health and prevent fractures.
Some of the approaches being explored include:
 Tissue engineering: This involves using scaffolds and growth factors to stimulate the growth of
new bone tissue and promote the repair of damaged bones. The goal is to create functional
bone tissue that can replace the lost bone mass and density in individuals with osteoporosis.
 Stem cell therapy: This involves using stem cells to replace the damaged bone cells and promote
the repair and regeneration of bone tissue. Stem cells can be taken from the patient's own body
(autologous stem cells) or from a donor (allogenic stem cells).
 Biomaterials: This involves using synthetic or natural materials to replace or augment damaged
bone tissue. Biomaterials can be designed to mimic the properties of natural bone and promote
the growth of new bone tissue.
 Gene therapy: This involves delivering a functional copy of a gene involved in bone growth
and repair to the affected bone cells. The goal is to restore the production of the missing protein
and improve bone health.
These approaches are still in the early stages of development, but hold promise for the future
treatment of osteoporosis. Clinical trials and further research are needed to determine the safety and
efficacy of these therapies.
In addition, traditional treatments for osteoporosis, such as medication, exercise, and
lifestyle changes, will likely continue to play an important role in preventing fractures and
maintaining healthy bones in individuals with osteoporosis.

3.4.4 Artificial Bones

Artificial bones, also known as bioceramic implants, are medical devices used to replace
damaged or missing bones. They are made from biocompatible materials, such as ceramics or
polymers, that mimic the properties of natural bone.
Ceramics:
Ceramics commonly used in artificial bone applications are biocompatible materials that
resemble the mineral component of natural bone. Some examples include:
Hydroxyapatite (HA): HA is a calcium phosphate ceramic that closely resembles the mineral
phase of natural bone. It provides excellent biocompatibility, osteoconductivity (abilityto support
bone ingrowth), and chemical similarity to bone mineral. HA-based ceramics are widely used in
bone grafts, coatings for orthopedic implants, and scaffolds for bone tissue engineering.
 Tricalcium Phosphate (TCP): TCP is another calcium phosphate ceramic that is similar in
composition to natural bone. It has good biocompatibility and biodegradability, allowing it to
gradually resorb as new bone tissue forms. TCP ceramics are commonly used in bone graft
substitutes and as fillers for bone defects.
Bioactive Glass: Bioactive glasses, such as silicate-based glasses, possess the ability to bond
with bone tissue through the formation of a biologically active interface. These glasses promote
bone regeneration and are used in bone grafts, coatings for implants, and scaffolds.
Polymers:
Polymers used in artificial bone applications offer flexibility, versatility, and the ability to
customize their properties. Some examples include:
Polycaprolactone (PCL): PCL is a biodegradable polymer with good mechanical properties.
It is often used in bone tissue engineering scaffolds due to its slow degradation rate, allowing it to
provide support during the regeneration process.
Poly(lactic-co-glycolic acid) (PLGA): PLGA is a biocompatible and biodegradable polymer
composed of lactic acid and glycolic acid units. It has been extensively used in various medical
applications, including bone tissue engineering. PLGA scaffolds can be tailored to degrade at a
desired rate, enabling synchronized new tissue formation.
Polyethylene Glycol (PEG): PEG is a hydrophilic polymer that can be modified to create
scaffolds with specific properties. It can be combined with other materials, such as ceramics, to
enhance their mechanical strength and bioactivity. PEG-based hydrogels and composites have
shown promise for bone tissue engineering.
Advantages
Artificial bones can be used to treat a variety of conditions, including osteoporosis, bone
fractures, and congenital conditions that result in missing or malformed bones. Some of the
advantages of artificial bones include:
 Durability: Artificial bones can be made from materials that are more durable than natural bone,
making them more resistant to fractures and other forms of damage.
 Customization: Artificial bones can be designed and manufactured to fit a specific patient's
needs, taking into account factors such as size, shape, and bone quality.
 Reduced risk of rejection: Unlike natural bone, which can be rejected by the body, artificial
bones are made from biocompatible materials that are less likely to cause an immune response.
 Faster recovery: Artificial bones can often be implanted more quickly than natural bone grafts,
which can lead to faster healing and rehabilitation.
However, there are also some potential drawbacks to artificial bones, such as the risk of
implant failure, long-term stability issues, and the need for additional surgeries in the case of implant
wear or damage. Overall, artificial bones are a promising technology that can provide a range of
benefits to patients with damaged or missing bones. However, further research is needed to fully
understand their safety and efficacy, and to develop new and improved artificial bone implants.