The Effect of Boiling On The Proximate Analysis and Mineral Composition of Okra
The Effect of Boiling On The Proximate Analysis and Mineral Composition of Okra
The Effect of Boiling On The Proximate Analysis and Mineral Composition of Okra
Article No.: 052224071 Okra fruits are one of the most commonly consumed vegetables worldwide
Type: Research for good health as well as nutritional benefits. The present work seeks to
Full Text: PDF, PHP, HTML, EPUB, MP3 determine the effect of boiling on the proximate and mineral composition
okra purchased from Wurukum market, Makurdi. Benue State. The samples
were washed with distilled water to remove sandy particles. It was then
sliced in to uniform thickness using stainless steel knife, oven dried and
Accepted: 28/05/2024 pounded into powder form and were analysed for proximate and mineral
Published: 21/06/2024 contents using standard methods. Results of proximate composition of both
raw and boiled okra is as follows: moisture content (12.20 and 12.82 ), Ash
*Corresponding Author content (8.45 and 7.80). Crude fibre (17.65 and 15.58 ), Crude protein (16.44
Gav, B. Lyambee and 14.89),Crude fat (2.40 and 2.15). The results revealed that boiling have
E-mail: benedictgav@ gmail.com effect on the proximate parameters. The results obtained from mineral
analysis of both raw and boiled okra revealed the presence of Ca (50.00 and
Keywords: Proximate, Mineral, 46.67 ppm), Fe(0.49 and 0.22ppm), Na(11.83 and 10.12 ppm), Mg(7.45 and
Analysis, Okra, Composition.
6.93 ppm) and Zn (0.09 and 0.04 ppm). The results of the mineral elements
were within WHO standard for food consumption. Generally the results both
the proximate and mineral analysis showed that okra were healthy for human
consumption.
Greener Journal of Agricultural Sciences, vol. 14, no. 2, pp. 73-85, 2024
74 Gav et al / Greener Journal of Agricultural Sciences
The samples were washed with distilled water to remove The moisture content was determined by an air oven
sandy particles. the samples were sliced using a method as described by (AOAC, 2000) Two grams of test
stainless steel knife. The moisture content each of the sample were weighed in duplicate into already weighed
Okra was determined immediately after sliced to uniform and cooled petri-dishes the sample were transform into air
thickness. It was then oven dried and ponded to powder oven at 1050C for 3 hours. At the end of 3 hours the
form. sample were allowed to cool in desiccator. It was taken to
the oven to dry and be reweighed until a constant was
Digestion of Samples obtain. It is calculated as:
The digestion of the samples was carried out by the Moisture constant
process described by Adedolu & Adewuyi (2013). Weight of sample (g)
Exactly 1.0g dried and sieved samples was weighed into Weight of dry matter (x)
25.0 mL conical flask. 12.0 mL of freshly prepared and Loss in weight (g-x)
aqua-regia (3ml HNO3 + 9 ml Hcl) was added. The flask
𝑔=𝑥 100
was covered with a filter paper to enable the digestion to % moisture content ( × )
𝑔 1
take place under constant volume. The constant was
heated for 1hr on the medium heat of a hot plate. The
mixture was allowed to cool and filtered through a filter
76 Gav et al / Greener Journal of Agricultural Sciences
Protein determination was determined by the method Crude fat was determined using AOAC (2000) method 5
described by A0AC (2000). Two-tenth gram of the sample g of sample were weighed into thimble and a loose plug of
was weighed into a Kjedhal digestive flask. Eight-tenth of fat free cotton wool was filted into top thimble with its
catalyst moisture was placed in a conical flask with few content was inserted into the bottom extractor of the
boiling chips. 10ml of concentrated H2SO4 acid was Soxhlet apparatus. A 250 ml round bottom flask of known
added and the mixture was heat on a heating mantle. weight contain 150-200 ml of petroleum ether and fat
Initially gently until foaming cease and content become extracted under reflux for about 3hrs. At end of the
completely liquefied. extraction the solvent was recovered. The mixture of
It was the heat vigorously until the liquid was clear extracted oil solvent was transfer to an air oven 100oC for
and free from black colour. The flask wa cool and the 5 mins, to move radiant moisture. The flask with the oil
constant diluted with twenty-five ml distilled water. was then cooled in a desiccators and weighed.
Distillation apparatus was connected, 5ml of boric acid
solution was measured into a 500 conical flask and a few crude fat
drops of methylene red indicator were added. The flask Weight of sample (g)
was placed on the receiver so that the end of the delivery Weight of fat (x)
tube tips just below the level of the boric acid.
𝑥 100
5 ml of digested samples was pipette into % fat ( × )
𝑔 1
distribution unit 7 ml of 50%, NaOH solution was added.
The unit was close and the liberated ammonia was
Determination of ash
streamed distilled into boric acid the unit was titrated with
0.1m HCL acid until the green colour changed to purple.
The ash of food stiff is the inorganic residue remaining
The percentage of nitrogen in the sample was calculated after the organic matter has been burnt away. The ash
with the formula obtain is not necessarily of the same composition of the
mineral losses due to volatilization of some interaction
Crude protein between components.
Weight of sample (g)
Titre value (T) Procedure:
The method described as outline in AOAC (2000) modified The crucible was cool in desiccators to room temperature
was used in determining the crude fibre content of sample, and then weighed.
two grams of sample was weighed into a 500 ml beaker
and boiled into 200 ml HCL (1%) for 30 mins. The The percentage as was calculated as
suspension was filtered using a white filter paper and Weight of sample (g)
rinsed with hot water to obtain filtrate. The residue Weight of Ash (x)
obtained was transferred into a crucible and placed in an
𝑥 100
oven for 30mins. The dried residue was cooled in a %ash ( × )
𝑔 1
desiccator and weighed. Percentage of crude fiber was
calculated as
Determination of carbohydrate
crude fiber
The procedure outlined in AOAC (2005) was used in the
Weight of sample (g)
determination of carbohydrate content.
Weight of dry matter (x)
weight of residue (y)
This was calculated by difference sum total of the
𝑥=𝑦 100 moisture, fat, protein ash content were subtracted from
% fibre ( × ) 100 as below.
𝑔 1
Parameters Raw okra Boiled okra Mean SD CV% WHO Stdrd Yr 2011
Ash content that the samples contains high ash content which
indicates that the okra pods would provide essential
The ash content is a measure/reflection of the valuable and useful minerals needed for body
nutritionally important mineral contents present in the development. The mean of ash content in this result
food material (Omotosho 2005; Nnamani et al., 2009). agrees with the findings of Adetuya et al., (2011) (7.19–
Table 4.1 shows the crude ash contents of raw and 9.63 %). And it is high above the reported WHO standard
boiled used in this study. The level of ash content was 2011 (4.1 %).
ranged from 8.45 % (raw okra) to 7.80 % (boiled okra) The difference between the ash content in raw
with a mean of 8.13 %. The ash content was significantly and boiled okra is shown in figure 4.2.
higher in “raw okra” (8.45 %), whereas the boiled okra
had a lower ash content (7.80 %). The results showed
Crude Fibre expands the inside wall of the colon, causing the
passage of waste, thus making it an effective anti-
Crude fibre provides bulk to the gut which stimulates constipation. Fibre also reduces the risk of various
peristalsis and result in shorter passage time and more cancers, bowel diseases and improves general health
frequent defecation. The proximate crude fibre content and well-being of individual. The mean value of fiber in
presents in this study was (17.65 % and 15.58 %) for the this study is higher than the result reported by World
raw and boiled samples and the mean value was (16.62 Health Organization standard (1.80). Table 1. revealed
%). According to Eromosele (2013), Fibre helps in the the mean value of raw and boiled samples and this was
maintenance of human health and has been known to higher than 11.8% reported by Olagunju (2014) and
reduce cholesterol level in the body. High fibre foods 8.02% result reported by Alawore (2014).
Calcium (Ca) boiled. The mean result is 48.33 ppm. Raw okra had the
highest calcium content (50.00 ppm) which was
Calcium is the major component of bone and assists in significantly higher than the Calcium content in boiled
teeth development. Calcium concentrations are also okra (46.67 ppm). This result appeared to be far less than
necessary for blood coagulation and for the integrity of the Calcium contents of Okra variety reported by WHO
intracellular cement substances (Okaka and (2011) which is 143.47 Mg/100g.
Okaka 2001). Calcium content in raw and boiled okra is The difference between the calcium content of
shown in Table 4.2. The concentration of Calcium in the raw and boiled okra is shown in figure 6.
sample is varied from 50.000 ppm in raw to 46.67 ppm in
Iron (Fe) infection (Ullah et al., 2012). Table 4.2 shows Iron
content of raw and boiled Okra. The contents of Iron
Iron is an essential trace element for hemoglobin varied from 0.49 ppm” to 0.23ppm”. the mean result is
formation, normal functioning of central nervous system 0.38. The Iron content of raw okra had higher (0.49 ppm),
and in the oxidation of carbohydrates, protein, and fats but this did not differ significantly from boiled okra” (0.23
(Kermanshah et al., 2014; Mlitan et al., 2014). It also ppm). The values obtained in this study were far less
facilitates carbohydrates, protein, and fat to control body than the value reported by WHO. (2011) which is
weight, which is very important factor in diabetes (Moses 5.50mg. This indicates that Okra pod is a rich source of
et al., 2012). Iron is necessary for the formation of Iron.
hemoglobin and also plays an important role in oxygen Figure .7: shows the difference between the Iron
transfer in human body and low iron content causes content of raw and boiled okra.
gastrointestinal infection, nose bleeding myocardial
82 Gav et al / Greener Journal of Agricultural Sciences
Sodium (Na) but this did not differ significantly (10.98 ppm) The values
obtained in this study were far less than the value
Sodium content of raw and boiled Okra are shown in reported by WHO. (2011) which was 17.82.
Table 4.2. In this study, the sodium contents varied from Figure .8: shows the difference between the
11.83 ppm in raw to 10.12 ppm in boiled. Sodium content sodium (Na) content in raw and boiled okra.
of raw Okra (11.83 ppm) was higher than the boiled okra
Benjawan, C., P. Chutichudet, and S. Kaewsit. 2007. Effect selected basil leaves of Ocimum gratissimum L, in
of green manures on growth yield and quality of green Libya. Int. J. Chem. Eng. Appl. 5:8–17. ]
okra (Abelmoschus esculentus L) har lium cultivar. Moses, A. G. , Gitu E. G. L., and Rotich H.. 2012. Profile of
Pak. J. Biological Sci. 10:1028–1035. heavy metals in selected medicinal plants used for
Dandena, G. 2010. Shifting to alternative food source: the treatment of diabetes, malaria and pneumonia in
potential to overcome ethiopias’ malnutrition and Kisii region, southwest Kenya. Global J.
poverty problems. Emilie Coudel, Hubert Devautour, Pharmacol. 6:245–251.
Christophe Toussaint Soulard, Bernard Hubert. National Research Council (2006) "Okra". Lost Crops of
Cirad-Inra-Sup Agro, Montpellier, France, 10 p. Africa: Vegetables. Lost Crops of Africa. National
Effiong, G.S., Ibia T. O., and Udofia U. S.. 2009. Nutritive Academies Press. Vermerris W, Nicholson R (2006)
and energy values of some wild fruit spices in south Phenolic Compound Biochemistry, Springer,
eastern Nigeria. Electron. J. Environ. Agric. Food Dordrecht, The Netherlands.
Chem. 8:917–923. Naveed, A., A. A. Khan, and I. A. Khan. 2009. Generation
FAO http://faostat3.fao.org/home/E mean analysis of water stress tolerance in okra
Gemede, H. F., N. Ratta, G. D. Haki, A. Z. Woldegiorgis, (Abelmoschus esculentus L.). Pak. J. Bot. 41:195–
and F. Beyene. 2015. Nutritional quality and health 205.
benefits of okra (Abelmoschus esculentus): a review. Ndunguru, J., and A. Rajabu. 2004. Effect of okra mosaic
J. Food Process Technol. 6:458. virus disease on the above-ground morphological
Gopalan, C., Sastri, SBV., Balasubramanian, S. (2007). yield components of okra in Tanzania. Sci. Hortic.
Nutritive value of Indian foods. National Institute of 99:225–235.
Nutrition (NIN), ICMR, India. Nwachukwu, E. C. , Nulit R., and Go R.. 2014. Nutritional
Habtamu, F., N. Ratta, G. D. Haki, A. Z. Woldegiorgis, and and biochemical properties of Malaysian okra
F. Beyene. 2014. Nutritional quality and health variety. Adv. Med. Plant Res. 2:16–19.
benefits of okra (Abelmoschus esculentus): A review. Nwofia, G. E. , Nwogu Victoria N., and Nwofia B. K..
J. Food. Sci. Qual. Manag. 33:87–96. 2012. Nutritional variation in fruits and seeds of
Jabeen, S. , Shah M. T., Khan S., and Hayat M. Q.. pumpkins (Cucurbita Spp) accessions from
2010. The typical symptoms of determination of major Nigeria. Pak. J. Nutr. 11:946–956.
and trace elements in ten important folk therapeutic Nzikou, J. M., M. Mioula-Tsieri, E. Matouba, J. M. Ouamba,
plants of Haripur Basin. Pak. J. Med. Plants C. Kapser, M. Parmentier, et al. 2006. Study on
Res. 4:559–566. gumbo seed grown in Congo brazzaville for its food
Kathirvel, P., and P. Kumudha. 2011. Chemical composition and industrial applications. Afr. J. Biotechnol.
of prosopis juliflora (SW.) D.C (mosquito bean). Int. J. 5:2469–2475.
Appl. Biol. Pharmaceut. Technol. 2:5–14. Ohr, LM. (2004) Dietary antioxidants. Food Technology 58:
Kermanshah, A. , Ziarati P., Asgarpanah J., and Qomi M.. 67-74. 64. Gosslau A, Chen KY (2004)
2014. Food values of two endemic Nutraceuticals, apoptosis, and disease prevention.
wild Almond species from Iran. Int. J. Plant Anim. Nutrition, 20: 95-102.
Environ. Sci. 4:380–388. Okaka, J. C. , and Okaka A. N. O.. 2001. Pp. 54–56 Food
Kermanshah, A., P. Ziarati, J. Asgarpanah, and M. Qomi. composition, spoilage and shelf life extension, ocjarc.
2014. Food values of two endemic wild Almond Acadamic Publishers, Enugu, Nigeria.
species from Iran. Int. J. Plant Anim. Environ. Sci. Oyelade, O. J., B. I. O. Ade-Omowaye, and V. F. Adeomi.
4:380–388. 2003. Influence of variety on protein, fat contents and
Kumar, R., Patil, MB., Patil, SR., Paschapur, MS. (2009). some physical characteristics of okra seeds. J. Food
Evaluation of Abelmoschus esculentus mucilage as Eng. 57:111–114.
paracetamol suspension. Intl. J Pharm Tech Res 1: Ullah, R. J. A. , Khader I., Hussain N. M., Talha A. M., and
658-665. Khan N.. 2012. Investigation of macro and
Kumar, S., Dagnoko, S., Haougui, S., Ratnadass, A., micronutrients in selected medicinal plants”. Afr. J.
Pasternak, D., et al. (2010). Okra (Abelmoschus spp.) Pharm. Pharmacol. 69:1829–1832.
in West and Central Africa: potential and progress on Uyoh, E. A. , Ita E. E., and Nwofia G. E.. 2013. Evaluation
its improvement. Afr J Agric Res 25: 3590-3598. of the chemical composition of Tetrapleura
Mlitan, A. M., Sasi M. S., and Alkherraz A. M.. tetraptera (Schum and Thonn.) Tuab. accessions
2014. Proximate and minor mineral content in some from Cross River State, Nigeria. Int. J. Med. Arom.
Plants 3:386–394.
Cite this Article: Gav, BL; Nanev, JD; Surma, N; Kutshak, PI; Odike, G (2024). The Effect of Boiling on the Proximate
Analysis and Mineral composition of Okra. Greener Journal of Agricultural Sciences, 14(2): 73-85.