22/05/2024 14:14:58

Copy of pAX01_T7Pol (10808 bp)

pG2_For (DNA) pMBP-LbCpf1 REV (DNA)

Fwd_D'_Coty (DNA)
Rev_A'_cotY (DNA)

00
100 pBE-S_RE
V (D p
NA) AX01
_L
b Cp
7 'LacA
- 964 AmpR f1
) 59 Amp-R lam F
90 bd
a
NA

WD
t0
(D

(D
Xy te
er

NA
l
im

-F

)
rm
i

ri
or
_Pr

R
in
2o
32 er

at
X_3

or
- m BR

k
3'
pGE

PCR p
p
ar
bom

X
A m p R yl
X
rop

0
pGE

pRS

A)
Pxyl_T7Pol_F (DN )
pr
44

A
o

PxylA_rev (DN
L4

+1

XylA Promoter
mo

ajout

2500
ter
l a m b d a t 0 2 ter rmi

PCR_Ori_

10808 bp
rrnB

l a c A m i n a i nat to

BL21
rrnB

col

07
'
ter
T

T7p

Pol

1
T1

479
T7
750

te
m
t o or

40-
r

R
0

PC
rrn

21
na

pBR
B-

r forEco
T1

T7 terminator
_R

erm
ev

PC
NA R
Ter tet pr T7 Term
(D

) min omoter Stop

ateu
r pBMP
pJM
P1 F
WD (DNA)

5000

T7Pol_MluI_R (DNA),+2
pMBP-LbCpf1 REV (DNA) Rev_T7_term (DNA)
pBE_term_R (DNA)

https://benchling.com/abrans/f/lib_UrEiwxL8-promoteur/seq_vD1kcJ0x-copy-of-pax01_t7pol/edit 1/1