Answers Biotechnology and Genetic Modification Worksheet No.1

ANSWERS BIOLOGY WORKSHEET NO.
1 (i) kills, bacteria / microorganisms / pathogens ; 1
(ii) any two from: 2

(heat) denatures enzymes ;
so lactose, not broken down / not digested ; MP2 A not optimum temperature, for
digestion / AW
changes shape of active site ;
enzyme will not fit substrate ;
(iii) lactase ; 1
1
Question Answer Marks Guidance
(iv) any one from: 1 ora throughout

can reuse the enzyme ;
cheaper ;
no enzymes left in milk ;
so milk does not need to be purified ;
AVP ; e.g. enzymes more stable / less likely to denature
/ affects taste / drinking the enzyme might trigger allergies
2
2 (i) S – amylase ; 2
T – maltase ;
3
(ii) C6H12O6 ; 2
2 (C2H5OH) + 2CO2 ;
(iii) 1 conserves fossil fuels ; 3

2 idea that fossil fuels are a finite resource / fossil fuels are not
renewable / biofuels are renewable ;
3 maize / plants, uses carbon dioxide (from the atmosphere) ;
4 ref to photosynthesis ;
5 idea that so balancing the carbon dioxide in, exhausts
/ emissions / AW ;
6 less likely to cause acid rain ;
7 ref to global warming / climate change / (enhanced) greenhouse
effect ;
8 uses waste from crops for energy ;
9 AVP ; e.g. reduces dependency on fossil fuels from other countries
4
3 (i) liquid / fluid / watery, part of blood ; 1
(ii) amino acids ; 1
(b)(i) plasmid ; 1
(b)(ii) restriction enzymes ; 1
(b)(iii) cutting / opening, A / the plasmid, with same (restriction) enzyme(s) ; 3

forming, sticky ends ;
idea that (sticky) ends of human DNA and plasmid DNA are
complementary ;
reference to, bases / base sequences (of sticky ends) ;
correct reference to (DNA) ligase ; e.g. inserting gene / sticky ends
joining / splicing
AVP ; e.g. B is a recombinant (plasmid / DNA)
(b)(iv) reliable / constant, supply ; 1

produce, large(er) quantities / in a fermenter / bacteria reproduce
quickly (to make more genetically engineered bacteria) ;
not dependent on blood donations ;
idea that no (named) health risk(s) ;
higher quality of product ;
AVP ;
(b)(v) mRNA moves through the cytoplasm ; 2

mRNA molecules, move to / through, ribosomes ;
sequence of bases in mRNA determines order of amino acids (in
TPA) ;
for protein synthesis / to make proteins ;
AVP ;
5
4 (a) yeast ; 1
(b)(i) flour / starch / sugar / glucose / sucrose / carbohydrate ; 1
(b)(ii) respiration ; 1
(b)(iii) carbon dioxide ; 1
(c) step 3 / 35 °C / low(er) temperature: 2

optimum / best / suitable / AW, temperature for,
respiration / enzymes / gas production ;
to allow the dough to, rise / expand / AW ;
step 5 / 200 °C / high(er) temperature

organism A / microorganisms / yeast, killed / enzymes denature ;
to cook the dough ;
to allow ethanol to evaporate ;
(d) biofuels ; 2
wine / beer, making ;
penicillin / antibiotic (production) ;
AVP ;;
6
5 (a)(i) restriction (enzyme) ; 1
(a)(ii) sticky ends ; 1
(a)(iii) plasmid is cut with the same (restriction) enzyme (as DNA) ; 3
to form, sticky ends / region A, that are complementary to sticky
ends of, gene / cry ;
reference to base sequences (being complementary / AW) ;
(DNA) ligase used to join plasmid and, cry / gene ;
AVP ; e.g. formation of recombinant DNA / plasmid
(b) gene / cry, codes for the sequence of amino acids in, (toxic) 4
protein ;
DNA / gene / cry, is copied / transcribed, to form mRNA ;
cry / gene / DNA, remains in nucleus ;
mRNA moves from nucleus to, cytoplasm / ribosome ;
mRNA passes through ribosomes / AW ;
ribosomes assemble amino acids (into protein molecules) ;
ribosomes make proteins ;
AVP ; e.g. order of amino acids determined by base sequence of,
mRNA / DNA / gene
(c) to kill / harm / deter, caterpillars / insects / pests ; 3

higher yield / reduce losses due to, (certain) caterpillars /
insects / pests ;
higher quality of cotton ;
use less insecticide ;
(so) less pollution ;
(so) less money spent on, insecticides / spraying ;
(so) less risk to workers’ health (from using insecticides) ;
(so) less chance of insecticide resistance ;
AVP ;
7
6(a) 1 nucleus / chromosome(s) ; 9

2 bases ;
3 protein ;
4 genes / alleles ;
5 same ;
6 sticky ;
7 recombinant ;
8 bacteria / vectors / viruses / yeast / (prokaryotic / host)
cells ;
9 insulin ;
(b) washing powders ; 2

(pectinase) for (fruit) juice production ;
(lactase) for lactose-free milk ;
AVP ;

Answers Biotechnology and Genetic Modification Worksheet No.1

Uploaded by

Copyright:

Available Formats

Answers Biotechnology and Genetic Modification Worksheet No.1

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Answers Biotechnology and Genetic Modification Worksheet No.1

Uploaded by

Copyright:

Available Formats

ANSWERS BIOLOGY WORKSHEET NO.

1 (i) kills, bacteria / microorganisms / pathogens ; 1

(ii) any two from: 2

(iv) any one from: 1 ora throughout

(iii) 1 conserves fossil fuels ; 3

3 (i) liquid / fluid / watery, part of blood ; 1

(ii) amino acids ; 1

(b)(ii) restriction enzymes ; 1

(b)(iii) cutting / opening, A / the plasmid, with same (restriction) enzyme(s) ; 3

(b)(iv) reliable / constant, supply ; 1

(b)(v) mRNA moves through the cytoplasm ; 2

(b)(i) flour / starch / sugar / glucose / sucrose / carbohydrate ; 1

(b)(iii) carbon dioxide ; 1

(c) step 3 / 35 °C / low(er) temperature: 2

step 5 / 200 °C / high(er) temperature

5 (a)(i) restriction (enzyme) ; 1

(a)(ii) sticky ends ; 1

(c) to kill / harm / deter, caterpillars / insects / pests ; 3

6(a) 1 nucleus / chromosome(s) ; 9

(b) washing powders ; 2

You might also like