®

Advanced Test Equipment Rentals

E stablished 1981 www.atecorp.com 800-404-ATEC (2832)

Nikon

INSIRUCIIONS

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 CAUTIONS
I AvoidStrongShocksl
ll afd e the microscopeg e n r l y ,t s k i n g
care 10 avoid strons shocks
E When Carryingthe Microscope
W h e n c a r r y n gt h € m i c r o s c o p es. ! p p o r l
t h e b o u o m o f t h - cm c r o s c o p eb a s e T h e
n s r r ! m e n tw e g h s . b o u t 7 9 k g D o n o t
ltsl!l!9!!9rl4sl!s fthebcse-
E Placeof Use
A v o d t h e l s e o f t l r €m c r o s . o p c n a d ! s r y
p r a c e ,o r w f e r c l m a r n e s l o l e c l l o
V b r a to n t o r c l p o s e dr o l . g r
lemper.r!rcs mn sir.e, .r o r.c:

4 PowerSourceVoltageand Fuse
a ' . . . - ' . . . . . . , . . , ' . . . : a g e . nfdu s e
-: :.. -t :-i. -..),'.... Q n
a LrghtSource
T h e h a g q e nb ! b r o b e u s e di s 6 V 3 0
I r _ : ' . . : . . . . . : r : r e . : . a n r l r - .o n e
:.:.'.. ". :i ..ci..a

f a b! b oi T..: i.a. .e slqgested

w a t t a g es L r s e d , l lf 'ee ! f : a d l u s t i n gc r c ut
ma! oe oamageo
6 Turningon the Lamp
T s k ec a f e n o t t o t o u c h t h e l a m pn o u s i n g
w h e n i t s i t , a n d d o n ' 1 b rq g i n f l a m m a b l e
s ! b s t a n c e s u c ha s g a s . n - . .t h i n n e ra n d
l r l c o h on € a rn . . s s o m ep a . t so f t h e a m p
h o l s n g m a y b € c o n r el e r y h o Lw h e t h e
Thank you very much for purchasing
a Nikon lMicroscope. This microscope Z Changingthe Bulband Fuse
is a high precisioninstrumentwith a B e f o r er e p a c . 9 t h e b L rb o r l u s e . t u f i
very delicatestructureand varied O F Ft h e m a n s w t c l r a n d d i s c o n n e ctth e
p o w e rs o u r c ep l u a . R e pa c €t h e h a l o g e i
functions.Pleasethoroughlyreadthis a m p 1 6 V3 0 W ) . m a k l n gs ! f e t l r a t t s
manualfirst to use the microscope c o o e n o u g h .D o n o l t o u c ht h e q l a s sp a r t
correctly. with Vour bare hands.
B Dirt on the Lens
D o n o i l e a v ed u s t .d i r t . o r f i n
t h e l e n so r b u b s u r f a c e s
Theyw I preventyou from c early
obsetuiigth€ specim€n
-91FocusKnobs
N . ! c r a t t . n p r r o ! d l u s t t h e t . J h t . e s so t
th€ rght !nd €ti fr!fd ro.!s k.oi,s !Y
t u r n i n o o n e , w h i i e h o l d i n atrh e o t h e r . r
n r ! c a ! s . p f o l i e m s D ! . o t t L r r .t h e
i d r s e l o c l s k r . . i b :p . s t t h e i m i t A ! . d
t r r r . n g t i . r . h ! , b e . . ! : e t n a , r c' a u s e

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 CARE
ANDMAINTENANCE
A Cleaningthe Lenses
T o c l e a f t h e e n s s l r f a c e s .r e m o v ed l s t
Lrsing a s o f t b r u s ho r g a u z e O n l yw h e n
r e m o v i n gf i n g e rm a r k so r g r e a s e .u s e a
s o l t c o l t o nc o t h , e n s t i s s l e , o f g a u z e
ghty moistened w i t h p u r ea l c o h o l
lmethy a cohol or ethyl alcoho ) IESL
sE4lra!|i s!i9!lry9! 9i "91
Lr only xylene. Do nol use xylen€for
c l e a i n 9 t h e s u . f a c eo f l h e e n t r a n c el e n s
of the eyepiecelLrbeor the pr sm sLrface CONIENTS
of the Ult.a Wide EyepieceTube UW"
O b s e r v es u f f i ce n t c a u to n n h a n d ln g
r . N o l \ , l E N C L A T U R. .E. . . . . . . . . 3
a c o h o la n d x y l e n el t h e y. r e n T T . A S S E M B L. Y . . .. .. . . . . . . . 5
f a m m a b l e )a.n d t h e O NO F Fo f r h € . 4 l c R o s c o. p. .y- . . . . . . . 1. 2
r nN
p o w e f s o u r c es w i t c h .
V . M A N I P U L A T I OONF E A C H
Z Cleaningthe PaintedSurfaces
Avoid the use of anv orqanic solvent (for E L E t v t E N. T
. . . . . . .. . . . . . . . . . .. 1 6
e x a m p€ , t h i n n € r .- " t h e r a
. c o h o) l o r s e v i c e .. . . . . 1 6
1 ) U s eo f f o c u s i n d
c e a r n g t h e p a n t e ds l | f a c e s . r d p l a s t c
p a r t so f t h e n s t r ! m - . n l W € r e c o n r m e n d 2) Optica pathchangeoverin the
y o ! ! s € t l r es i c o f c l o t h . tr noculareyepecetulre 1f
g NeverAttemptto Dismantlel 3) D opteradlustmenr . .. .... . 1 8
Never attempt to dismantlethe 4) nterpupillaryd stance
i n s t r u m e n bt e c a ! s ey o u m a y m p a r t h e
adjustment . . ,. . ,. ,. . ,. ,. J8
.

@ When Not in Use 5) Centerng the condenserens T9

W h e n n o t i n L r s ec. o v e ft h e i n s t r u m e n t 6)Useoffeddiaphragm ... 20
w l f r t h e a c c e s s o rvy n y c o v e r .a n d q l e l 9
it in a place free from moisture and 7) Useof condenserapenure
f u n q u s . t i s e s p e ca l y r e c o m m e n d e d d a p h r a s m . .. .. . . . . . 2 0
that the objectves and eyepeces be kept
i i a n a i . tg h t c o n t an e r c o n t a i n i n g 8 )U s eo ff i t e r s- - - - - . - . - - 2 1
9 ) O i m m e r s i omn a n i p La rl o n . . . . 2 3
El renoorc Lnecttncl B L E S H O O TGI N. " ' , . . , . 2 4
V ,TROU
To mainlainthe b€st pe.formanceof the
i n s t f t r m e n iw. e r € c o m m e n dt h a t t h e
ELECTRICS APL E C I F I C A T I O2N9S .
instr!m€nb l e p e f o d l c al y c h e c k e d ( F o r
d e t a s o f t h s c h e c k , c o n t a c ty o u r a u -
t h o r i z e dN i k o nd i s t rb u t o r .)
*Ple.se nole .rs pe. you. N kon warcntv,
''Any defects or damage dircctly or indi
rectly caused by the use of unauthorized
replacementparts and,/or performed by
unautho zed personnel" wil vod the

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 I NOMENCLATURE

E y e pe c e 1 ! b e c l a m ps c r e w

S p e c m € n( g l a s ss r d e l

S!bstage

C o n d e n s ecr e n t e r l n gs c r e w

Y a x s s l a g em o l i o nc o n t r o

X a x l ss t a l e m o t o n c o n i r o

F e d d i a p h r a g irrn g

L r n Fi o t a g e . d c a d l n p d r e
L a m ph o u s i n gc o v e f
L r n F! o l t a q e s e c L t o r s w 1 . h

F r g .r - |

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 NOI\4ENCLATURE

B n o u l a re y e pe c €t u b e

D o p t e rr n g

R e v o l vn g n o s e pe c e

d l a p h r a g mr i n g

C o n d e n s ecr a m p s c r e w

C o f d e n s e fr o c u s k n o b

Fig.l 2

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 il. ASSEM B LY r'o""r,ns
anddismantrins)
To assemblethemicroscope,follow the proceduresgivenfrom Oto(?
Fordelails,readp.6 to p. 1'1.

1u

r@

@
@
Fig.2

Tools: hexagonwrench (accessory),plus screwdriver, minus screwdriver

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 N.ASSEI\4BLY

O Checkthe PowerSourceVoltageand Fuse

o Dlsconnect the powercord from the AC inputconnector.(Make
surethat t rs disconnected.)
. Makesurethatthelinevoltageindication plateatthe rearofthe base
indicates the correctine vo tageto be used (1OO-120Vor
2 2 O - 2 4 O V\ )F i s . 2 1 ) .
When t indicatesLncorrecl voliage,rerriove
the ne voltage
indicationplateby unscrewingthe two screwsusinga plusscrew
driver.
. Mount the ine voltagendicationp ate afterswltchng lhe input
vo tagechangeoversw tch to lndlcatecorrectvo tage.

Fg. 2-1 F i s . 22

a Remove thefuseholderat the boitomofthe baseus ng a plusscrew

driver(Fig.2 2) and nrakesurethatthe metalpartof the fuse
"250V "250V T1 tot 224 244V.
nd cales 1A' for 10O 120V or A'
When ncorrecifusetype s to be nserted,pleasecontact
your aulhorized Nikondisirbulor.
.Insert ihe fuseintothe fuse holderand iightlyscrewit intothe
origina positon usinga plus screwdriver.

O LevelingFoot Screw
. Forstableinstallation
of the microscope,man pulatetheadjustment
screwat lhe rear-righiofthe base(Fg. 2 3).

Fig.2-3

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 N.ASSEMBLY

a3)MountingLampand LampHousing
Keepthe coveron or useqloveswhenmountinqthe ampbulbso
as not to touch th9 !!r[q!g dilcq!]yt{llluyelrillgels-Lf i.rc9l!]4!!
or dirt on the bulbsurface, wipethemoff with lenstissue.J\4ake
5!l€ te removethe cover after mountinq the lam
. F - . m o v c l h e a m p h o L r s n gc o l e r p r e s s I g t h e c o v e r d e r a c h f g
b u t t o n s ( F g2 4 1
a n s e n t h e a r n p i n t o t h e s o c k e l p I h o e s L l r t t f e a c h e st h e r n t t
(Fs2 5)
. R e a t t a c ht h c c o v c r

tr-ts
_^4
[fr
tF i g2 4

Cautionswhen dismantling
. T u f f o l i t h e p o w e rs r , ! t c l rl M a k - o s L r r e i ri asi o f f . ) D o n o t t o u c h
t h e b u l b i m m e d i a t e l v a f t e r t u r n i n qbiet oc iaf u s e sn v e r y h o tM . ake
suretharlhe bu b has coolodenouili \ryhenfeplacrngt

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 II,ASSEIVIBLY

the Objectives
G) lVountingand Dismantling
al'4ounttheoblectvesntothe hoes olthe revolvlngnosepece n
such posilons that, when viewedfrom above,theirmagnfving
power ncreases is revoved c ockwise(Flg 2 6)
as the noseplece
Cautionswhen dismantling
. Removethe ND f ler cassette ii altachedand lowerthe stageby
turfing the coafsetocusknob.
aRemoveany specimenf t is on the stage
. Ho d lhe oblectivewith both handsso as not 1c)drop 1 when
removng lt from the revoving nosepiece.

F i g . 26 Ftg27

O l\/lounting
the Stage
a Lowerlhe sLrbstage by tLrrnng the coarsefocusknob
a Loosenthe stagec amp screwsuffcienty.
a Insertthe stageLntothe c rcu ar dovelallof lhe stagernountol the
substage,ll and E. Fastenthe stagewrlh the stagec amp screw
3(Fs.2 7).
Specimen Holder
aThe specmen ho der shoLrd be lasienedto the mo!nt ng no e on
t h e r i g h t s l d e f o reaf t k n o b s l a g e a n d o n t he ef l s d e f o r a r g h kt n o b
stage 8e'novethe speclmenholderwhenobservnga arge

and Dismantling
@ lVlounting Lens
the Condenser
. Raisethe substageby tLrn ng lhe coarsefocusknob
a Lowerthe condensercarrler11]ts lim t by turn ng the condenser

.lnsertthe condenserens nto the condensercarrlerEwth the

numercal aperturep atefac ng towardthe user Fastent wlth lrre
condenserc amp screw2 (Fg 2-8).
. RaLsethecondensercarrierto focus
ts im tbyturningihecondensef

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 N,ASSEI\1BLY

Cautionswhen Dismantling
.q- oeLe\Df r" e n et d d "n e o
. Lowefthe condensercarrer to Ls imil by turn n! the
fccLrsknob.Removethe
condenser lensbyre easingthe

i.--,-=-,.r,!4

Fis.2I Fis.2 I
_
V o u n l i n g t h e B r n o c ua r E v e p i e c T
eube
o L o . s e f i h e e r ' c p. . e t u b e c n r r p s c f e v rs | r l l c r e n i! u s r f g t h e
h.ria!of lref.h lSee,? l
. n s e r tr h e c f . ! a r d o i e t i r o f t r e t f n . r c ! i f e f e p e c e t l r b c f 1 0 t h c
c r . L r a f d o v e i a c i t h . . r y - ! pc . e L r b e r o ! f l F a s i , .I i { , t h t h e
e y - a pe c e l | r b e c a n r p s c r c \ \ i F ! r 2 9 )
Cautionwhen clamping
. Do fot ho d th. p asi c p.ft ofthe hexa!.rna ,.,/fench,,vhEn
l g i r i c n f q L h ee l e p e c e t u b c . a . n p : . r e w H o a L h el t e n o l t h e
\\refch f oveflalhtefed, opt c: path .hang.i ov.r of the
e y e p e c e t L r b . m a \ rb e n a i L r f r coi n e d

I l\.4ounting
the Eyepiece
. U s e i h e s a m . m n g n I c a t o f e y e pe c e sl o f b o t h r h c I g h i a f d

. n s e r tt i r c c y - a pe c e s f t o i h e s e e ! . s o i t h e b l l o . i L r l aer y - a pe . - . L u b - .
A '

pr.nrtrsrolls
ol the sleeve1Fg 2 10)
o\'VhcnLrsrng
eyegrardrLrbbefs,nsertth.m nt.lhe eyepeces(Fq
2 11).

-<(d::
/#l}lrm--
I / -<?):. - \./ | rq,
/ 1l--J'--r--
,/

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 N.ASSEIV]BLY

@ Placingthe Filteron r h o F i 1 1 6 r e 6 ^ a h i . . 1 6
P acerhe rr45mm
filteron the f her receptacleof the fie d lens part.

Fig.2-12

@ Mountingand Dismantling
the ND FilterCassette
(optional)
a Pushdo!!n the ND fiter cassettefrom thetop so thetwoprotrusons
oi ilre lowerpan of the ND f rer cassettefit wlth the two mounring
gfooveson the r m of the f eld ens partofthe mrcroscope base
l F i s . 21 3 ) .
aWhen freqlenty lowerngthe m croscopestage,mountthe ND
{iter cassette in the d rectionas shownin Fig. 2 14.
.When dismanding.pushlhe cassette eitherio the eft or rightand
ra se the opposte side

Fig.2-13 Fis.2-14

10
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 X.ASSEIV]BLY

O Connectingthe PowerCord
a nsenthe socketof the powercordto the AC npul connectorand
plug it into a AC linereceptacle (Fig 215).
oWhen the receptac e is not a double-poe groundtype, usea plug
c o n v e n n ga d a p t e(rF i g . 2 1 6 ) .

F i s . 21 5 F i g .2 1 6

@ HexagonWrench
.The hexagonwrench s storedin the backofthe
Take t out as shown r F i g 2 1 1 .

--=l

Fis.2 17

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11
 N. MICROSCOPY
1) Putthe NCB 1 1 filler(or necessary f ter)on the filter
recepiace of ihe f eld ens part (Fig. 3 1).

F i s . 31
i{ used{Fig.3 2).
2) Mountthe ND flltercassefte,
(Beferto [.ASSEIV]BLY @ p.10)

3)Turn on the mainswitchto ightlhe lamp.

S idethe br ghtnessadjuster to meetwiththe 3rd ine
from the right. (Fig. 3 3).

F€.3-3
4) S ide the f ler insertion/ removalknobsof the ND
filtercassene to the imitto placethe entireND fiter
in the opticalpath(Fig. 3 4).

Fis.3-4
5) Bevove the revoving nosepieceto pul the TOX
objectivento the oplcal path. Properly turn the
revovingnosepiece so itfts lnto position(Fq. 3 5).

6) P acethe specinren(g asss ide)on the stagewith ts

coverglassfacingup andfastenitwiththe specmen
h o l d e r( Fs . 3 6 ) .

12 F i 9 . 36

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 rn.MrcRoscoPY

7 ) R . s et h €c o i d e n s e e r nsLrsnglhecondenserloc!sknob
! n t i t h t s t h €l m t ( F i g 3 7 ) . W l r e n l s i n g a n a c h r o m a t c
a p l a n a ct o n d e n s e ra. d d o i a k n g c a r €i o t t o a l o w
b u b b l e st o e x s t b € r w e e n
t h et o p o f t h ec o n d e n s elre n sa n d

( S e ep 2 3 . M a f p ! a r o n o f E a c hE e m e n t9 ) )

Fis.31

(Fis 3 8)
d aphrasms

F i s . 3I
! l M . n p u . t e t h - " Y a x i s a f d X a r s s t a g - "m o t o r c o r t r o l
knobsto pltthespec mlr fiolrreoptic. pair iF g. 3 !).
(Pace the ipe.rm-.. .bo!. llrc .oridenser eis l

I0)When ire tr io.! ar !,.ep e.e t!be s !sed, cfraf!le

i h e o . t . a . . t f , o l : r e e r ' . p c . c 1 ! b e t o e n t - e rl O l l l l i
01thc !tht fto t.-. b n..! er pa.l
' l l .

1 1 ) N . 4 apnu L a t teh e c o a f s c l n e l o c u s k i o b a r d T o c L rosn

r h e o o k i n g I t h e e y € pe c El F g . 3 1 O )
t h e s p e c l m e lw

F i g . 31 O
2) Adluslrhe diopter(Fig. 3 'l I )
(Seep 18. N.4an pu at on of EachE ement3).)

(Bm)
I w
13) Adjuslthe nterpup ary d stance(Fg 3 12)
( S e ep . 1 8 . M a np u l a t o no f E a c hE e m e n t4 )

L
Fig.3-12
- 13
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 In. tvltcRoscoPY

1 4 ) F o c u sa r d c e n t e r t h ec o n d e n s e re n s ( F i g .3 l 3 ) .
{ S e ep . 1 9 , M a n l p u l a t o no f E a c hE l e m e n t5 ) . )

1 5 ) B e v ov e t h e r e v ov i n g n o s e p i e c leo t h e o b l e c t v et o b e
u s e da n d l o c u s o n t h e s p e c i m e nb y m a n r p ua n n gl h e
f i n e f o c u so r c o a r s el o c u s k n o b . ( S e eN o t e : 2 . )
W h e n u s i n ga n o l Li m m e r s i o no b i e c t i v ea, d d o I t a k n g
care notto a low bubblesto existbetlveenthe top ot the
o b l e c t v €a n d t h e s p e c l m e n ( S e ep . 2 3 , l v l a np u l a t i o n
o f E a c hE l e m e n S t ).)

F i g . 31 3
1 6 ) W h e nt h e N D f l t e r c a s s e l t e luss e d ,A d t u s t t h eb n g h t n e s s
b y s i d l n gt h e f l t e r n s e a r o n , / r e m o v ak n o b o f t h e N D

( S e ep 2 1 , M a n i p u l a l i o o
n f E a c hE L e m e n8r) . )
When the ND fiter cass€tteis not used. adjustthe
b r g h t n e s so f t h e a m p b y m a n p u a t i n gt h e b r i g h t n e s s

I7) Adlu$ the v e\44eld d aphragm and the apenure

d i a p h r a g mb y m a n i p ua l i n gt h e i rc o n l r o lr i n g s .
{ S e ep 2 0 , M a n i p ua t o n o f E a c hE l e m € n 6 t )and 7).}

14
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 III. I\4ICROSCOPY

Note 1 : Manipulate the centering

screwsof the condenserlens if part of the viewfield
is dark. lf this doesn'thelp, checkthe followingitems:
. nse(on ,/ femova of the ND nr. N4tcFoscoPY
4)
. Turnng the revolvn! trI N,4CROSCOPY
5)
nosepece(clckstop posrtion)
. Postron of the cofdensertens IN. MICROSCOPY7)
. Viewfeld and aperrured aphragms III I\4CROSCOPY
8)
. Changeoverof the opticatpathof lur MtcRoscoPY
10)
the eyeptece tube
' M o u n t i n gt h e l a r np I L A S S E M B LOY M o u n tn g r h e
amp and lhe amp housng
. Mouni ng the condenser II ASSEMBLY i0 Mountingthe
' Placifgthe fiter on the liter rL ASSEMBLY(t P ac ng ihe filter

Note2: Checkthe Jollowingitemswhen tne specrmencannotbe focused.

. Mountng the spec|rren III M CROSCOPY 6)
. Thicknessof rhe covef
glass(standafd=0. 1Tmml

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15
 N.MANIPULATION
OFEACH
ELEMENT
1) Use of FocusingDevice
Theturn ng d recion of the knob and the directlonof vertcal
movementof the stageare shown n Fig. 4.
Onerotatonofthef ne focusknobmovesthestage0.1mm andthe
graduationon the finefocusknob s I micron. One rolationof the
coafsefocls knob movesthe stage12mm. The rangeof coarse
and f ne motion is 2mm up and 28mm down from rhe standard

Thecoarsetocusknobtightensby turnlngthetorqueadjustment r ng
counrercocKWrse.
Neverturn the riqht or left knob while holdinq the other. lt mav
causeprootems.
Do not turn the coarsefocus knob Iurther than the limit.

Ftg.4

16
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 Y, IV]ANIPULATION
OF EACHELEIV]ENT

2) 0ptical
PathChange-0ver
intheTrinocular
Eyepiece
Tube
Note)Binocular
eyepiecetube B" does not haverhe opticalpath
change-over function.10O%of the lightentefsthe observation

tube "F"
(1) When usingthe trinoculareyepiece
A s s h o \ / n n F i g .5 I . w h e n t h e o b s e r v a r i o n r u i sbt e
u r n e dt o w a r d
the Lrser,100% of the lighrentersthe obsetuat on tube.
A s s h o w nn F i g5 2 , w h e n t h e o b s e r v a t o n r usbree v o l v e6d0 ' l e f t ,
1 0 0 % o ft h e l i g h l e n t e r s r h e v e r tpi h
cao t o t u b e1. ne i r h ecf a s e t. u r n
the tube to the limit.

Fis.51 Fts.52

tube 'T"
(2) When usingthe trinoculareyepiece
A s s h o w n n F g . 6 . w h e nl h e c h a n g eo v e rk n o bi s p u s h e du n t t
reachesthe imit llt, 1O0%of the ightentersthe observarion rube
When the changeoverknob feachesthe nlermediate cick E, rhe
proponon ol I ght efteringthe observarion rubeand phototubew l
be 14r86 When the changeoverknob is pu ed ro rhe mll El .
000; rato -. a t6p oo L o

O b s e r v a r ornu b a 1 O 0 %

Fig.6

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17
 N, I\,IANIPULATION
OF EACHELEI\IENT

3 ) D i o p t e rA d j u s t m e n t
(Beforeadjustn.r the diopter, mp emenl1"4 CROSCOPY pfocedures
I I to 1 1) afd focuson the specimenwih lhe 10X oblectve)
N / o r 6 o o o , . t d o . - - - r - .t q l o , I o -, g
' ' ' ' p d o p
o ' lo o d L n 1 q o E o , 6 b p E 6 h E 6 o
surfaceof the r ng co nc des with the engraved ne
l T hs s t h c p o st o n o f 0 d o p tc c o m p e n s a o tn .) ( Fg 7 1 )
(21Sw iS the 40X objectiveby tlrrnng the revov ng nosepece and
b r n g t h e s p e c i n r emna g en t o f o c u sb y t u r nn g t h e fn e f o c L rksn o b
(or the coarsetocLrsknobJ
( 3 ) S w i ntgh e4 X o r 1 0 Xo b l e cvt e n t o p o s t o n . W t h o L r t m a n i p u l a t i n q
the {ine and coarsg-focus knobs,turn the dioplerr ngs on lhe
eyepecesso thatthe specmen imagesin the rlghtand left
eyepecesare focused nd vidualy(Fg. 7 2).

Flg.1 1 Fig.l-2

a Repeatthe aboveproceduretwo t mes, to adjustthe dlopter

. o 4 b o o a d j ur - - n o n p a o g d r o p" . o . , . . o- -..
the Lrsersrighl and efteyes,wi keepthetube engthofthe
m croscopecorrect Thisenabes the Lrser to takefLr advantage of
the h gh quaiityobjectives,nc Lrdng the I parfocalty.

4) InterpupillaryDistanceAdjustment
(Beforeadlustng the nterp!p arydslance,irnplemenlM croscopy
procedlrre1) to 12) and focus on the specimenw th the 10X
objectve)
Adluslthe nlerpup ary d slance,so that boththe flght and left
v e$4ieds becomeone (Fig 8)
Th s adlustmenl w I enablethe userto observewth both eyes

1B
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Fig.8
 Ni,MIANIPULATION
F E A C HE L E I V ] E N T

5 ) C e n t e r i n tgh e C o n d e n s eLr e n s
lBeforefocLrsing nnd centerng the condenserers, rmplemenr
M r c f o s c o p y p r o c e d ! f e f rl o) tm
o l 3 ) a n d t o c u s o n r h e s p emce n w r h
the I 0X objecrive.l
(1I C ose ihe f e d d aphragl.fto irssrnalesl sizeby man pu ar ng rhe
ie d di;phragmcontro I nq. Rotatethe co|lder]ser focLrsknob to
movethe ccndenserverticaly so ihat a sharp .iage ol the f e d
djaphragrns formedon the specmen sLrrface (Fg 9 I ,2 afd 3)

l,hi
! '
I

i,1..; Eyep ece vle!{.field stop

F g . g1 F iigg. .gg2 F t g . g3

(2) f the nragedecenters irom the v e',,,.f

-.ldof the eyepiece,bring i
roughlyto the cenierol lhe ! e\,!iied by rneansol rhe condensef
cenierng screu,siF g I 0 I and 2).
13)Ch:ngeto the 40X objectv,. FocLrs oI Lhespecmen by tLrfnng
t h ef l r e f o c l r sk n o ba f d f o r ma n m : g e o f t h e f e d d a p h r a g m o n
the specmen sLrdace by nranpLratifg the condenser focusknob.
r l w - p , o . h o . ^ a
1lo t-recenterof the! ev\4e d by meansofthe condenser cefter ng
scr-.\",'s At thrst me, the centerifgwit befac raredbyadj!sIfg
t h e s z e o l t h e l m a g eo f r h e f e l dd a p h r a g mt o b e s t g h r y s m ae r
llian the v ewfc cj of the eyep-.ceby manipuai ng the v ewfe d
d a p h r a g ncro n t r or n g l F i S .I 0 3 ) .

F i g . 1 01 F i g . I 02
91O-3

19
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 V . M A N I P U L A T I OONF E A C HE L E I V ] E N T

6 ) U s eo f F i e l dD i a p h r a g m
Thefie d diaphragms usedfor determlning lhe i um natedareaon
the specmen Thes ze of the dlaphragmis adlustedby manip! at ng
t h e f e d d a p h r a g m c o n t r or n g . S t o p p i n q d o w nt h e d i a p h r a q m to
such an extent that the circqrfglelcc qtlh€ lllulll q
circumscribesor inscribesthat oJ the evepiecefield ol y!€tq s
recommended for genefalmicroscopy f a w der areathan requred
n t e f t h eef d o f v e w , c a u sn g f l a f e
s l u m i n a t e de ,x t r a n e o u s l L g hl et w
n the imageand owerng the coftrast Therefore,especaly n
pholomcrography,the properadl!rstrnent of the {eld d aphragm s
verylmpofiant Generaly,good resuts w ll be achlevedwhen the
d aphragms stoppeddownio suchan extentthatthe d ameterofthe
luminatedareais s ightlyafgerthanthe d agonalof the f m format

7 ) U s eo f C o n d e n s eAr p e r t u r eD i a p h r a g m
The condenseraperluredlaphragmis providedfor adjustng the
fumerica ape{Lrfe(N A.)of the !l !m natingsystemoi lhe
,-. , - t6..Tm n e 5 e r e o . ,o , . i "
i m a g e .c o n t r a s ld, e p t ho f f o c u s .a n d b r g h t n e s s .
Stoppng downthe aped!rediaphragmw ll owerthe resoluton and
b f g h t n e s s b L nr tc r e a steh e c o n t r a satn d d e p t ho f l o c u s .I n g e n e r a .
when lt is stopped down to 70% to 80% of the numericalape4llq
of the obiective,a good lmageof appropfate contrastw I be
obtained(Fg. l 1)
To adlLrst the s ze of the condensefapertured aphragm,manpu ate
t h e d a p h r a g mc o n t r or i n gr e i e r r n gt o t h c c o n d e n s e rN ' s. A . s c a e .
o r a f t e r r e m o v n g t h e e y e p i e c e lt fhoeme y e p e c e t u b a ed, l!stthesze
of the d aphfagm,observng the imageof the diaphragmthat s
visb e of the br ght c fcleof theexl pupilontheinsde oftheobjective
Sloppingdown the apenurediaphragmtoo tar w I o\/er the
resov ng power Therefore,it ls recommended not lo slop down the
apertureto a s ze smaler than 60% of lhe N A oftheobjectve n
6, 60 .ho,
,6 oo o,. gor-lo

Size oithe condenseraperturediephr.gm

Fi9 1 1

20
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 I V .M A N I P U L A T I OONF E A C HE L E I \ I E N T

8) Use of Filters
(1) ND filter cassette(Optional)
Accessoryftersaitachedto lhe opt onalNDflter cassette
areND2,
ND4, and ND16. Theseafe usedfor generamicroscopyand
br ghtnesscontro n photorncrography.
ND2
ND4
ND]6 fransm ssio. r.te=abolt ti"/.

. Light s reducedto 118whenthe ND2 and ND4 fiters are put nto
the opticalpathand is reducedto 1,/64whenN D4 and N D 16 f ters
are pul inio the opticalpath. Lightcan be reducedfrom 1/2 to 1/
128 dep€ndng on the comb nationof the flhers.
. Securey changethe f ter nsertion/remova knob.
Removingand mountingthe ND filters
use qlovesor qauzeso as not to touch the filterswith your bare
nanos.
o o r o . o o N D r t . r .o . o i . l o r , u r " 9 " . - o a d p t
Placethe ND tillercassette on it and spreadthe ever{Fig. 12).
To mountthe f ller, ifseri the f ter obliquey n the opposites de of
the everand spreadlhe leverfrom the lowers de
Any fiterswith an oulerdiameterof 45mm and th cknesslessthan
3rnm can be mountedin the ND fiter cassette.The indicationon
thefter nsertion// removalknob, however,caf'tbechanged

Fig. 12

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21
 V. I\IANIPULATIO
ONF E A C HE L E M E N T

Transparent
Cover
l\4ounllhelransparentcover lo protectthefllefsfromdust(Fig.13-1)
Whenthe ND filtercassettes removed,attachthe tfansparent cover
on the fie d Lens(Fg. I 3 2) However,removethe coverdur ng
observai on or phoiomcrographng w th the v e\,4e d d aphragm

F t s .1 3 2

(2) Filterreceptacle
F ltefswth an outerd ametefof 45mm can be p acedon the f tef
r e c e p l a c l e o f t h e t e d d a p h r a g r n p af rt th.e t h c k n e s os f t h ef l t e r
s l e s s t h a4n . 5 m m , N D f l t e r c a s s e tct ae nb e m o u n r e w d i t ht h e l l l e r
p acedon the f ler receplace.
-h-r6. .6 6 p, -n t -
.,. ,-. , h " 5ho..n h- "bl-
beloware convenefty usedplacifg them on the f ter receptac
e.

F o rl e . e r a m . r o s c o p !a n d . . . r
NCB ]

F.r m.f.s..pr oi rrhase

' t ut ! r
G , c : f n L e , J c r e- e
n r o n o . i r o m ep n . i o n . r o ! f a p n y

F o rh e m a t o r yn e o s n s t an n O
HE

22
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 ]V. I\IANIPULATION
OF EACHELEI\,1ENT

(3) Otherfilters
. C33mrn Lemof sk n f tcr
W h e r i t h e o p i o n n l i r c h r o m a tc o n d e n s e r s L r s e d .i n s e r t t h . d 3 3 m m
e m o f s k n I t e r i n t o t h e r - o c e p L aec b e t w e e f t h e c o e c i o f e n s a n d
t h e a l r i pb ! b . ( F g . l . + l )
. d45mm heai absorbng i rer
W i e r i t h e h e a t a b s o f b i n g f l l e r i s ! s e d . n r o ! n t t i t o L h ed i l L r s e r
ho dff nseded nro lhe botiom ot the m cfoscope base

F l g .l 4 l Fig. 14 2

9 ) O i l l m m e r s i o nl \ 4 a
n i p ul a t i o n
"o
Tfe oblectv.s wth the ndicriiof a f ! t o b e n r n r - os e a l i o
b e t \ , l e e nl f r e s p e c m c n a n d t h e f r o n l - a r s o i l h e o b l e c i v e L l s e o n \ i
. -cluded oi .W 49 o 6 o o,
: p e r t l r f e I 0 o r a r g - a r , 1 s r e c o m m e i d e d l o L r s e : n o r n r n e f so r i
t y p e a c f r r o m a t a p a n a t c o f d e n s e r 1 0 n r a k ef L r L r s - o f 1 ' s e J fc i e n c y
F o r t h s L y p -o" f c o f d e n s e f ,a p p y o b o . c n t h . s p . c m c n a n d t h c

A p p l v t h e m n n r u m a m o ! n l n e c e s s a r y( t h e q ! a f t l y t h a l I i s t h e
l r n t r l J e t w - . e ft r e l f o f t o l t h e o b l - . c t v a a n d t h e s p a c m - o n o r
b e t v r e . , nt h c f r o n l o f t h - ac o f d e f s e r e r s a r d t h e s p e c i m e n lt o a \ , o r d
a I o v r o l e x c e s s\ , e o I t h n i r ' I a d h e r e t o t h e s h l l e d i d t h e
( ) r c u f i i l e T e f c eo Tt r e c o n d e n s e f e n s
T o s e e f . I b L r b be s a r e p r e s e f r t r t h e r m m e f s . r n o r , w l r c h
l e t e r . r f a t e t h e m a g e q L r a l l y . p ! o ! 1 l h - oe y e pe c e k o n i L h e
. r y c pc c t t r b e . F u y . r p e n t h e v e w f - . l dd a p h f a g m a n d t h - .
a p e d u f e d a p h r a g m t o e x a m n e t h e o b l e c L v ee x 1 p r p ( b r g h t
.i rc e) if s de llre tube
T o r e m o v e a I b u b b e s , s l i g h t l \ r 'o t a l e t h e n o s e p e c e s e v e r n I m e s
1 o ft u r n t h e c o n d e r s e r f o c l s k n o b t o m o v e t s g h t l y u p a n d
d o w n ) . o r n p p y a d d r lo f a o r , o r r e p a c e t h e o
A f y r - . m n a f t o t o e i t o n t h e o I m r . r e r so f o b l , o cvt e o r a d h e s o n
o l o l i o t h c f r o n l o f l h e d n / s y s t e mo b l e c t v e \ / d e t e r i o r a t et h e
n a g e q u a r t ! C e a n o l l t h e o i a f l e r L t sn g 1 . n d n i . k e s L r r et h . t
r h e o i d d f o L a d i e r e t o t h e f f o n t o l o t h e r o b l e c tv e s
h e o l o r t h e c o n d c n s c r e n s s h o u d a s o b e c - o a n e do f l a f t e r ! s - o
T o c - o a no f f l h e o . w p e \ ' v d h e f s t s s | r e o f a s o i l c o t h , m o l s t e n e d
w l h r y e n e . ! l h l y t w o o f t h f e e t m c s o ! , c rt h c c n s l t s e s s - o n t l r
. t i h s i m e i o a v o . l t o L r c hf g i h e - a n : i h i r p a r t ( ] f I s s L r eo r c o t h
23
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 V . TROUBLESHOOTING
SEEING
ANDOPERATION

Oplca path ntrlnocuart!benolfity

r e optica path
C h a n g e o v et h
s e c u r c yr o D i o . ! a r
O p t . . p a t h n t r n o c ua r t u b e n o t
c h a n g e . lo v e rl o r f r f o c u l a r 11)

F e v o v n g n o s e p e c en o t i n c l c k s l o p B e v ov e l t t o c l c k s t o p p o s i t i o n
p o s t o n ( o b l e c l l v feo l c e n t e r e dn ( p u to b j e c t ! e . t o o p t i c a
parh). (p 12)

P o s to n c o r e c t y s o t h e
t o n d e . s e , l 5t o o o w v effre d daphragm mage s
form€d. (p 19)

C o n d e n s enr o t c e n t e r e d Centering. (p 19)

Condenser not mounted cofteclly N 4o u n rc o r r e c t y . (p 8)

t or t h € m t .
Chlr.rgeove
N D f i l e r n o t f u l y c h a n g e do v e r
\p 21)
F i ed d a p h r a g mc o s e d t o o m u c h O p e np r o p e r y (p 20)

m p r o p e rc o m b n a t o n o i o b j € c t i v e
U s pp . o p e rc o m br . t o n .

D a ro r d l s t o n t h e e n s ( f e l d e n s ,
c o n d e n s € ro, b j e c tv e . e y e p i € c e .
c € a nn g lp 2\
e y e pe c e l ! b e e n l r a n c ee n s )o r

L a m pn o t m o u n t e dc o r € c t l y \ / l o L r npt r o p e ry . { p 7)

24
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 T R O UB L E S O
HOTING

P o s t o n c o r f e c l ys o r h e
Po t u. of co.de ert!! o^ v e w Te l d . l a p l r r a g m n a g e s
lormed lp ]gi
A p € r t L r rdea p h r a g mr o o . e s v c L e d O p e np r o p e r l y tp. 2A)
D r r o f d l s i o n t h e e n s 1 1 cd
e n s , c o n d e n : - c ro, D t c c t r v e ,
C - . a ni l t tp. 2l
. y e p e . e , - e y eep c el ! b e e n t r a n c e

AtpF rc.lrrhfJgm loo rF ll! rd. op€i prop-.ry (p 20)

Positoi .orect y so llre

P o st o n o f c . n d e n s e rl o o o w v - . { r f i ed d . p h r a g m n r a g c s
formed (p 19)
T o o t l r i c l o r l l r n c o v e f ga s s L l s - .s p e c i f i e .i lh c k f e s s ( 0 I 7
No cover! ass atta.h-.d
N C Go b l e . t i v ef . r o b s e r !f ! l U s e n . r m a o b t e c tv e l o r
s p e . m e . \ ! r n o u i c o l e r o a s s! s e . l t o obsetuiq spec m-.n \,!rth
o b s . n e s p e cm e f t r ' t f r . o l e r g a s s
N o r n r a o r l c . t i ! e f o r o b s e r !r i q
sp.c m-entrrin cover! ass !s-Ed1o
obs-.^,espe. nrcn witlrolt

N o n r m ! r s i . no r L r s - .odf l r r el r o f t o f
m m € r so n s y s l e r no b l c c t ! e U s e N k o n m m e r so n o
(p 23)
mmersof o !sed noithe type

A r b ! b b e s , n n r r n c r s i oo
n B e m o l eb L b b . s lp 23)

s lirc ropof dt,

s t r s t e mo b l e c t ! e ( . s p e . . y 4 O X ) C eanrng lp 23]

C o f i r p e r s aot . r n g o f o b l c c t! c n o t
A d l ! s t t o m l r t c hc o l e r g3 s s

D fi or.lusl or tre leis li€ d ens.

. o n d - p n s e ro, b l c c n l e ,e ! € p - c c e ,
e y e pe . e t ! b e e n t r s n . el e n s )o r C e a nr ! 1 . lp 2)

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25
 V. TROUBLESHOOTING

;-,.-^^ i;:-- .,

Fevolvingnosepi€cenot in click stop Revolveit to cllck stop

position. (p. 12)

Place it stably n specrmen

i m a g ei s d i m . S p e c i m e nr s e s f r o m s i a g es u r f a c e .
h o l d e ro n s t a s e . lp 12)
S t a g et i t e d . Attach correctly. (p.8)
Revolvlngnosepiecenot n clrck stop Bevolvelt to c ick siop
postlon. \p.121
PLaceit stably in specrmen
Speclmen risesfrom stage surtace.
whilebeins h o L d eor n s t a s e . \p 121
Condenserlens not correctlY (p. 1 9 )
Cenle.ing.

S t a g et l t e d . { p . 8)
NCBII fiter not used. UseNcBl 1 filt€r. (p. I2)
lmage tinged
Lamp power source voltagetoo low. Adlust the amp voltage bY
m a n i p ua t i n gt h e b r i g h t n e s s
adluster .Use ihe optiona ND
l m a g e t o ob r i g h t . Lamp powersourcevo lage too high fiter cassetteif the conslant
co or temperaturers 10 De
Lamp power source vo tage too Low.

Apertured aphragm too restct€d. Openpropery. (p 20)

Not bright Positioncorect y so the
Positionoi condensertoo low. v i e w f i €d d i a p h r a s mi m a g ei s
Electrical). formed. (p 19)

Changeoverso thai 100% of

O p i i c ap a t h c h a n g eo v e r o t t n o c u l a r ight enters b nocLrLar
part.
t u b e n o t 1 O O %b i n o c ua r . (p.17)

26
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 T R O UB L E S H O O T I N G

Altach 10 stage w tlr coverg ass

! p ( w n e n . o c o v e r ga s s ,
spec me. s!rface sho! d be
!p) lp 12)

c o v e r ga s st o o l l r c k U s ec o v e r ga s s o l s p e c r e d

A i l a c ht o s l a g ew t h c o v e r ga s s
u p ( w i e i n o c o v e r ga s s
s p e c i m e ns L r . i a c es h o Ldr b e
!p) (p l2)
Llsecoverqass of spec iled
C o v e r q l a slso o i h c k .
t h c k n € s s( 0 1 7m m ) .
Erepie!c d opler .or ad !st€d Diopler a,:llLrs]ment. (p le)

E y e p i e c ed o p i e r n o t a d l ! s l e d

S d e h o d e r n o i t g i r ly f a s l e n e d1 r r

m p r o p e r, r l r d L i r . n e!nf i s d e l r o l d e r . S h I t l l r e a l t a . h m e n l p o s l i o n

A d l l s t i n t e r p u pa r y d s l a . c e
n l e r p ! p i l a r yd s l a n c en 0 1a d j u s t e d .
(p 18)
E y e pe c € d o p t e rn o l a d j l s t e d D o p l e ra d l ! s t m e n l (p l8)
M a s nf c a t o n s o i r g h l a n d
L l s es a m ee y e pe c e s ( p .9 )

Adllst nterpLp lary d slafce.

n l e r p L r pa r y d s i a n . e n o i a d j u s t e d
(p r8)
E y e p i e c ed o p l e r n o l a d l ! s t e d D o p r e fa d l ! 5 r m e n l (ple)

A d j u s tl l r e l a m pv o l a s e b y
m a n l p !a l . g t h e b r g h l n e s s
a d l ! s t e r U s et h e o p t i o n aN D
I n a d e q ! a t e| ! n r n a to n
T ter cassetteand adjusttlre
b r g h t n e s s w i t h c o mnba l o n o i
ND f lters ( p .2 1 )

27
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 V. TROUBLESHOOTING

ELECTRICAL
*r*"" ] C-;.

No electrcity obtained C o n n e ct h e c o r d t o s o c k e t .
A n a c hL a m p . (p 7)

lighteven though Bub blown. Feplacem€nt (p 7)

Replacement. (p ri)
Lamp not cofiectry mounted A u d c hs e . L r r F l y (p. 7)

Replacement. (p. 1 )

Connect power source cord

C o n n e c t onr o t s e c u r e . (p.ll)
securey

F u s eh o l d e rn o l f r m l yf a s t e n e d Fastens€cufely (p. 6 )

B u l b i n s u f i c i e n t l yn s e r t e d n t o t h e (p 7)
P o si l v e c o n n e c t i o n .

Bulb immediately
lamp.
Use6V 30W halosen
Bu b Lrsednot the one specified (p 2e)

I n p u tv o t a g ec h a n g eo v e rs w i t c h
C h a n g e o v enr p u tv o t a g e
does nor malch th€ powervo tage of (p. 6)
c h a n s e - o v esrw i t c h

F u s eu s e dn o t t h e o n e s p e cf e d . Us€therusespecfed. (p 6)

28
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 E L E C T R I C ASLP E C I F I C A T I O N S
Powersource . .......... 1OO-120V/22O 24OV, SOI5OHZ
Halogenlamp .....'..... 6V 3OW lspecialtypemadeby
PHILIPS]
Fuse . . . . . . . . . . ' . . . . . . 1. .O O - 1 2 O V r 2 b O
t AV
'l1A
22O-24OY:25OY
Powerconsumption... lessthan 43W

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29
 Nikon rcserves the right to make such ahera
t':ons in design as may be considered necessary
in the light of experience. For this reason.
paniculdB and illus att@s io th6 handbook mbl
/@t confom in every detail to modets in cutrent

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 NIKONCORPORATION
Fuji Blds.,2 3, Marunouchi3 chome, Chiyoda-ku,Iokyo 1o0,Japan
TeL 3133216-1026 T.l.* J226O1(NIKON) F.* A1€-32fi 5456

NIKONINC.
hshlmem Group. S!reyins Dept
1960rHd ton Av6nu6,Toiisce, califomia 90502 1309,Us.A.
Te[ (2|3) 516-7124T€l€* 967306(NKONGRCY)Far: (213)516251

NIKONEUROPE
B.V.
Schipholweg321, P.O.Aox222,1170 AE Badh@vedorp,The Nethe ands
TeL 020 659!106 Terer: 13323 {NTKONNL) Fa* O20-6s93335

rs
M027 rsr.s.erH.p
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