Chemistry Labsheets - Fall, 2022

AMERICAN INTERNATIONAL UNIVERSITY – BANGLADESH (AIUB)
Department of Natural Science (Chemistry)

Faculty of Science and Technology
Program: B.Sc. Eng’g (EEE/CoE/CSE/IPE)
CHEMISTRY LABSHEETS
CHEM 1101: CHEMISTRY (EEE/CoE/CSE/IPE)
(Quantitative Inorganic Analysis)

Volumetric titration: Acid-base titration, Redox titration, Estimation of Cu & Fe
Total hardness of water and Conductometric titration
List of the Experiments :
1. STANDARDIZATION OF SODIUM HYDROXIDE (NaOH) SOLUTION WITH STANDARD OXALIC

ACID (C2H2O4.2H2O) SOLUTION.
2. STANDARDIZATION OF HYDROCHLORIC ACID (HCl) SOLUTION WITH STANDARD SODIUM

HYDROXIDE (NaOH) SOLUTION.
3. STANDARDIZATION OF HYDROCHLORIC ACID (HCl) SOLUTION WITH STANDARD SODIUM

CARBONATE (Na2CO3) SOLUTION.
4. DETERMINATION OF TOTAL HARDNESS OF WATER USING ERIOCHROME BLACK T (EBT) AS

INDICATOR.
5. STANDARDIZATION OF SODIUM THIOSULPHATE (Na2S2O3.5H2O) SOLUTION WITH STANDARD

POTASSIUM DICHROMATE (K2Cr2O7) SOLUTION.
6. ESTIMATION OF COPPER CONTAINED IN A SUPPLIED SOLUTION BY IODOMETRIC METHOD.
7. DETERMINATION OF FERROUS ION (FE2+) IN A SUPPLIED SOLUTION OF IRON SALT BY

STANDARD POTASSIUM DICHROMATE (K2Cr2O7) SOLUTION.
8. DETERMINATION OF STRENGTH OF A WEAK ACID (CH3COOH) AGAINST A STRONG ALKALI

(NaOH) SOLUTION BY MEASURING CONDUCTANCE.
9. STANDARDIZATION OF POTASSIUM PERMANGANATE (KMnO4) SOLUTION WITH STANDARD

SODIUM OXALATE (Na2C2O4) SOLUTION.
10. DETERMINATION OF FERROUS IONS (FE2+) IN A SUPPLIED SOLUTION OF IRON SALT BY

STANDARD POTASSIUM PERMANGANATE (KMNO4) SOLUTION.
Text: 1. M. Mahbubul Huque and A. Jabber Mian, “Practical Chemistry”, 2nd ed. (1972), Student Ways, ISBN: Not
found. References: 1. J. Mendham, R. C. Denney, J. D. Barnes and M. Thomas, “Vogel’s Text Book of Quantitative
Chemical Analysis”, 6th ed. (2000), Pearson Education Ltd, ISBN: 81-7808-538-0; 2. G. H. Jeffery, J. Bassett, J.
Mendham, R. C. Denney, “Vogel’s Text Book of Quantitative Chemical Analysis”, 5th ed. (1989), Longman
(ELBS), ISBN: 0-582-25167-2
Name: …………………………………………………….., ID No: …………………, Section (Group): ………..
AMERICAN
INTERNATIONAL
UNIVERSITY –
BANGLADESH (AIUB)
EXPERIMENT NO. 1: STANDARDIZATION OF SODIUM HYDROXIDE (NaOH)

SOLUTION WITH STANDARD OXALIC ACID (HO2C-CO2H, 2H2O) SOLUTION.
OBJECTIVE: To know the strength of a secondary standard solution (for example, NaOH)
against a primary standard solution by acid-base titration.
THEORY:
(i) Methods: Acid-base titration,
(ii) Reactions: HO2C-CO2H + 2NaOH = NaO2C-CO2Na + 2H2O
(iii) Indicator: Phelophthalein
APPARATUS:
Burette (50mL), pipette (10mL), conical flask (250mL), volumetric flask (100mL), watch glass,
pipette filler, dropper, Stand and clamp etc.
REQUIRED CHEMICALS:
1. Supplied NaOH solution
2. Standard oxalic acid solution
3. Phenolphthalein indicator
PREPARATION OF APPROX. 0.1N OXALIC ACID SOLUTION. Transfer approx. 0.63

gram of pure oxalic acid (HOOC-COOH.2H2O ) in a 100 ml measuring flask and then dissolve it
with distilled water up to the mark. Normality of the prepared acid solution will be calculated as
follows:
Weight taken (in gm)  0.1

Strength of oxalic acid solution = (N)
0.63
PROCEDURE: Take 10 mL of NaOH solution in a conical flask by means of a pipette and

dilute it to about 50 ml. Add 1-2 drops of phenolphthalein indicator to the solution. Then add
standard oxalic acid solution drop by drop from a burette. Shake the flask frequently while
adding the acid solution. Stop the addition of oxalic acid solution as soon as the pink color of the
solution just disappears. Note the burette reading. The burette reading should be taken carefully
at the lower meniscus of the liquid. Difference of the initial and final burette reading gives the
volume of the acid added. The process should be repeated at least thrice. Take the mean of the
readings. Calculate the normality of the supplied NaOH solution.
May, 2022 Chemistry Lab Sheet

(Expt.1 contd.)
EXPERIMENTAL DATA:
Table: Standardization of supplied NaOH solution against standard oxalic acid solution by acid-
base titration.
No. of Vol. of Vol. of Oxalic acid (burette reading) (in Mean (in mL)
reading NaOH ( in mL)
mL.) Initial Final Difference
1 10
2 10
3 10
4 10
CALCULATIONS:
Strength of supplied NaOH solution:
VNaOH x NNaOH = VOxalic acid x NOxalic acid
RESULTS:
Students should know

• What are gram-equivalent weight, normality and molarity?
• Atomic weight, molecular weight of NaOH and HOOC-COOH, 2H2O
• Why phenolphthalein is used?
• Reasons behind the change of colour.
Text: M. Mahbubul Huque and A. Jabber Mian, “Practical Chemistry”, 2nd ed. (1972)

AMERICAN
INTERNATIONAL
UNIVERSITY –
BANGLADESH (AIUB)
EXPERIMENT NO. 2: STANDARDIZATION OF HYDROCHLORIC ACID (HCl)

SOLUTION WITH STANDARD SODIUM HYDROXIDE (NaOH) SOLUTION.
OBJECTIVE: To know the strength of HCl solution (being a solution made from secondary
standard substance) against a previously standard solution by acid-base titration.
THEORY:
(i) Method: Acid-base titration
(ii) Reactions: 1. HO2C-CO2H + 2NaOH = NaO2C-CO2Na + 2H2O
2. NaOH + HCl = NaCl + H2O
(iii) Indicators: Phenolphthalein, Methyl orange
APPARATUS:
REQUIRED CHEMICALS:
1. Supplied NaOH solution
2. Standard oxalic acid solution
3. HCl acid solution
5. Methyl orange indicator
(A) Standardize the supplied NaOH solution as in Experiment No. 1

Strength of oxalic acid solution = (N)
0.63
Table-1: Standardization of supplied NaOH solution against standard oxalic acid solution by acid-
base titration.
No. of Vol. of Vol. of Oxalic acid (burette reading) (in Mean (in mL)
reading NaOH ( in mL)
mL) Initial Final Difference
1 10
2 10
3 10
Strength of supplied NaOH solution:

VNaOH x NNaOH = VOxalic acid x NOxalic acid

(Expt.2 contd.)
(B) Preparation of approximately 0.1N hydrochloric acid solution:
Take 10 ml conc. HCl in a 1000 ml measuring flask and add distilled water up to the mark.
PROCEDURE: Take 10 mL of NaOH solution in a conical flask by means of a pipette and

dilute it to about 50 mL. Add 2-3 drops of methyl orange indicator to the solution. Then add
previously prepared (approx. 0.1N) HCl acid solution drop wise from a burette. Shake the flask
frequently during addition of HCl acid. Stop the addition of HCl acid solution as soon as the
yellow color of the solution just changes to orange or pink. Note the burette reading. Repeat the
process at least three times and take the mean of the readings. Calculate the strength of the dilute
HCl solution and from there calculate the strength of commercial HCl.
EXPERIMENTAL DATA:
Table-2: Standardization of supplied HCl solution against standard NaOH solution by acid-base
titration.
No. of Vol. of Vol. of HCl (burette reading) (in mL) Mean (in mL)
reading NaOH ( in Initial Final Difference
mL)
1 10
2 10
3 10
4 10
CALCULATIONS:
(A) Strength of supplied dil. HCl solution:

VNaOH x NNaOH = Vdil. HCl x Ndil. HCl to be determined
(B) Strength of conc. HCl solution:

Vdil. HCl x Ndil. HCl determined = Vconc. HCl taken x Nconc. HCl to be determined
RESULTS:

• What is normality and molarity?
• Atomic weight, molecular weight and gram equivalent weight of NaOH, HCl and
HOOC-COOH, 2H2O
• Why phenolphthalein and/or methyl orange are used?
• Reason of using methyl orange instead of phenolphthalein.

AMERICAN
INTERNATIONAL
UNIVERSITY –
BANGLADESH (AIUB)
EXPERIMENT NO. 3: STANDARDIZATION OF HYDROCHLORIC ACID (HCl)

SOLUTION WITH STANDARD SODIUM CARBONATE (Na2CO3) SOLUTION.
OBJECTIVE:
To know the strength of HCl solution (being a solution made from secondary standard substance)
against a weak base like Na2CO3 by acid-base titration.
THEORY:
(i) Method: Acid-base titration,
(ii) Reaction: Na2CO3 + HCl = NaHCO3 + NaCl (pH ~9.0)
NaHCO3 + HCl = NaCl + CO2 + H2O (pH ~4.0)
(iii) Indicator: Phenolphthalein, Methyl orange
APPARATUS: Burette (50mL), pipette (10mL), conical flask (250mL), volumetric flask
(100mL), watch glass, pipette filler, dropper, Stand and clamp etc.
REQUIRED CHEMICALS:
1. HCl acid solution,
2. Na2CO3 solution,
4. Methyl Orange indicator
PREPARATION OF APPROX. 0.1N Na2CO3 SOLUTION: Transfer approx. 0.53 gm of

anhydrous Na2CO3 in a 100 mL measuring flask and then dissolve it with distilled water up to
the mark.
Strength of sodium carbonate solution = (N)
0.53
PROCEDURE: Take 10 mL of Na2CO3 solution in a conical flask and dilute it to about 50 mL.
Add 1-2 drops of phenolphthalein and titrate against dilute HCl solution (prepared as expt. no.2)
contained in a burette. Now note the burette reading when just one drop of HCl discharges the
pink color of the solution. This is the first end point. Then add 2–3 drops of methyl orange inside
the same conical flask and continue titration against the same HCl solution. The end point
reached when the yellow color of the solution just changes to faint pink (or orange). Note the
burette reading. This is the second end point. The difference of the burette reading from initial to
second end point will be the volume of the acid required for titration. Repeat the whole
experiment 2-3 times and take the mean reading initial to second end point. Take last reading
without using phenolphthalein. Calculate the strength of supplied dilute HCl solution and then
find out the strength of concentrated HCl.

(Expt.3 contd.)
EXPERIMENTAL DATA:
Table: Standardization of supplied HCl solution against standard Na2CO3 solution by acid-base
titration.
Vol. of HCl (in mL)

Difference
Vol. of
No. of between Mean
Na2CO3 ( in
reading 1st End- 2nd End- (a) and (c) (in mL)
mL) Initial (a)
point (b) point (c) (in mL)
1 10
2 10
3 10
4* 10 ---
*4th reading with methyl orange only
CALCULATIONS:
(A) Strength of supplied dil. HCl solution:

VNa2CO3 x N Na2CO3 = Vdil. HCl x Ndil. HCl to be determined
(B) Strength of conc. HCl solution:

Vdil. HCl x Ndil. HCl determined = Vconc. HCl taken x Nconc. HCl to be determined
RESULTS:
Student should know:

• Is Na2CO3 a primary standard substance?
• Tell atomic weight, molecular weight and gram equivalent weight of HCl and Na2CO3.
• Can you use methyl orange first instead of phenolphthalein? If not why?
• Can you calculate the normality and molarity of HCl and Na2CO3?

AMERICAN
INTERNATIONAL
UNIVERSITY –
BANGLADESH (AIUB)
EXPERIMENT NO. 4: DETERMINATION OF TOTAL HARDNESS OF WATER

USING ERIOCHROME BLACK T (EBT) AS INDICATOR.
OBJECTIVE: To determination of total Hardness of water using Eriochrome Black T (EBT) as

indicator.
THEORY:
(i) Method: Complexometric titration. A complexometric titration is based on the formation of a
complex between the analyte and the titrant. The chelating agent EDTA is very commonly used
to titrate metal ions in solution. These titrations generally require specialized indicators that form
weaker complexes with the analyte. A common example is Eriochrome Black T for the titration
of calcium and magnesium ions to calculate hardness of water (see Lab Manual page at 21-23).
Ethylenediaminetetraacetic acid (EDTA) form a chelated soluble complex when added to a

solution of certain metal cations. If a small amount of dye such as Eriochrome Black T (EBT) is
added to an aqueous solution containing calcium and magnesium ions at a PH of 10.0 ± 0.1, the
solution becomes wine red. If EDTA is added as a titrant, the calcium and magnesium will be
complexed, and when all the magnesium and calcium has been complexed the solution turns
from wine red to blue, making the end point of the titration.
(ii) Reaction: (Y= deprotonated part of EDTA, In=EBT Indicator)
Titration reaction: HY3–(aq) + Ca2+(aq) → CaY2–(aq) + H+(aq) (also for Mg2+)
End point reaction: HY3–(aq) + MgIn–(aq) → MgY2–(aq) + HIn2–(aq)

wine red sky blue
(iii) Indicator: Eriochrome Black T (EBT), a complexometric indicator.
APPARATUS:
pipette filler, Litmus paper, dropper, Stand and clamp, Spirit lamp etc.
REQUIRED CHEMICALS:
(1) 0.01M EDTA solution,
(2) Conc. HCl acid,
(3) NaOH solution
(4) Buffer solution (pH 10)
(5) EBT indicator
(Expt.4 contd.)
Preparation of 0.01M EDTA solution (100 ml): Weigh out accurately 0.372 g of di-sodium
EDTA (C10N2O8H14Na2.2H2O) powder into a 100ml. volumetric flask and add a little water to
dissolve it. Make up to mark with distilled water.
Strength of EDTA solution = (M)
PROCEDURE:
Take the standard EDTA solution into the burette. Take 50 ml. of sample water (tap water) by a
volumetric pipette into a conical flask. Acidify the sample water with conc. hydrochloric acid
(use a litmus paper) and boil for a minute to drive off carbon dioxide. Cool and neutralize it with
sodium hydroxide (use litmus paper). Add about 2 ml of buffer solution (pH 10) and 2 drops of
EBT indicator. Titrate the solution with a standard 0.01M EDTA solution until the color changes
from wine red to blue. Repeat the titration at least two times and take the mean value. The mean
of three readings of EDTA is the volume of EDTA required to calculate the total hardness.
EXPERIMENTAL DATA:
Table-2: Determination of total Hardness of water using Eriochrome Black T (EBT) as

indicator.
Vol. of EDTA
No. Vol. of supplied Mean (in
(burette reading) (in mL)
of reading water (in mL) mL) (V)
Initial Final Difference
1 50
2 50
3 50
4 50
CALCULATIONS:
1 ml 0.01 M EDTA solution  1.00 mg of CaCO3 (as shown below)
So, total hardness as mg CaCO3 per liter of water = (a x b x 1000) ÷ ml of sample taken
[where, a = ml. of titrant required (mean volume of EDTA solution)

b = mg CaCO3 equivalent to 1.00 ml EDTA titrant.=1mg]
or, b= 1×molarity of EDTA0.01M
RESULTS:

• What are hard water and soft water?
• What are temporary and permanent hardness of water?
• Define the terms Chelete and Polydendate legand.
• What are household and industrial problems due to hard water?

AMERICAN
INTERNATIONAL
UNIVERSITY –
BANGLADESH (AIUB)
EXPERIMENT NO. 5: STANDARDIZATION OF SODIUM THIOSULPHATE (Na2S2O3)

SOLUTION WITH STANDARD POTASSIUM DICHROMATE (K2Cr2O7) SOLUTION.
OBJECTIVE: To know the strength of Na2S2O3 solution (being a solution made from secondary
standard substance) against standard K2Cr2O7 solution by oxidation-reduction titration.
THEORY:
(i) Method: Redox titration
(ii) Reaction:
1. K2Cr2O7 + 14HCl + 6KI = 8KCl + 2CrCl3 + 7H2O + 3I2
2. 2Na2S2O3 + I2 = Na2S4O6 + 2NaI
(iii) Indicator: Starch solution
APPARATUS:
REQUIRED CHEMICALS:
1. 12% KI solution,
2. NaHCO3,
3. Conc. HCl acid,
4. Standard K2Cr2O7 solution
5. Na2S2O3 solution,
6. Starch solution
PREPARATION OF APPROX. 0.1N POTASSIUM DICHROMATE SOLUTION.
Transfer approx. 0.49 gram of pure K2Cr2O7 into a 100 mL measuring flask and then dissolve it
with distilled water up to the mark.

Strength of K2Cr2O7 solution = (N)
0.49

(Expt.5 contd.)
PROCEDURE:
Take 4 mL of 12% KI solution in a conical flask and dilute to about 50 mL. Add about one gm of
NaHCO3 and shake the flask until the salt dissolves. Add 4 mL conc. HCl acid and then add 10
ml standard K2Cr2O7 solution by means of a pipette in the same flask. Shake the flask and cover
it with a watch glass, allow the solution to stand for about five minutes in the dark (inside the
desk or dark chamber). Rinse the watch glass and dilute the solution about 100mL. Titrate the
liberated iodine with sodium thiosulphate solution from a burette until the brown color fades
(light yellow). Add about 1 mL starch solution and continue titration by adding sodium
thiosulphate solution from the burette until one drop of the sodium thiosulphate solution changes
the color of the solution from deep blue to light green. This is the end point. Repeat the whole
experiment 2-3 times. Calculate the strength of sodium thiosulphate solution.
EXPERIMENTAL DATA:
Table: Standardization of supplied Na2S2O3 solution against standard K2Cr2O7 solution by oxidation-
reduction titration.
Vol. of Vol. of Na2S2O3

No. of (burette reading) (in mL)
K2Cr2O7 Mean (in mL)
reading
(in mL) Initial Final Difference
1 10
2 10
3 10
CALCULATIONS:
Strength of supplied Na2S2O3 solution:

V Na2S2O3 x N Na2S2O3 = V K2Cr2O7 x N K2Cr2O7
RESULTS:

• What are redox reaction, oxidizing agent and reducing agent?
• What is the difference between acid-base and redox indicator?
• Why it is necessary to keep your experimental solution in the dark?
• Is it iodometric or iodimetric that you are performing?
• Tell molecular weight and gram equivalent weight of K2Cr2O7 and Na2S2O3.
• What is the function of starch?
• Can you calculate the normality and molarity of K2Cr2O7 and Na2S2O3?

AMERICAN
INTERNATIONAL
UNIVERSITY –
BANGLADESH (AIUB)
EXPERIMENT NO. 6: ESTIMATION OF COPPER (Cu) CONTAINED IN A SUPPLIED

SOLUTION OF COPPER SALT BY IODOMETRIC METHOD.
OBJECTIVE: To determine the amount of Cu+2 ions in a supplied solution of copper salt by iodometric
method.
THEORY:
(ii) Reaction:
1. 2CuSO4 + 4KI = 2K2SO4 + 2CuI  + I2
2. 2Na2S2O3 + I2 = Na2S4O6 + 2NaI
(iii) Indicator: Starch
APPARATUS:
REQUIRED CHEMICALS:
(1) 12% KI solution, (2) Copper salt solution (3) NaHCO3 solid,
(4) 6M NH4OH (5) Conc. HCl acid, (6) Conc. CH3COOH
(7) Standard K2Cr2O7 solution, (8) 10% NH4SCN solution, (9) Na2S2O3 solution,
(10) Starch solution,
(A) Standardize sodium thiosulphate solution as Expt. No. 4.
Table-1: Standardization of supplied Na2S2O3 solution against standard K2Cr2O7 solution by

oxidation-reduction titration.
Vol. of Vol. of Na2S2O3
No. of (burette reading) (in mL)
K2Cr2O7 Mean (in mL)
reading
(in mL) Initial Final Difference
1 10
2 10

Strength of K2Cr2O7 solution = (N)
0.49
Strength of supplied Na2S2O3 solution (S): Vthio x Nthio = Vdichromate x Ndichromate

(Expt.6 contd.)
(B) Estimation of Cu ions:
PROCEDURE: Pipette out 10 mL of copper salt solution into a conical flask. Add 3-4 drops of
6M NH4OH until a faint permanent ppt remain and then add 6-8 drops of conc. CH3COOH. Now
add about 10 ml of 12% potassium iodide (KI) solution and titrate the liberated iodine against the
standard sodium thiosulphate solution (standardized previously) until the brown color of iodine
changes to light yellow. Add approx. 1 mL of starch solution, solution turns intense blue and
continue titration till the blue color begins to fade. Now add few drops of 10% ammonium
thiocyanate solution and continue titration until the blue color is just discharged (off-white).
Calculate the amount of copper present in 500 mL of copper salt solution.
EXPERIMENTAL DATA:
Table-2: Determination of the amount of copper in a supplied solution of blue vitriol by iodometric
method.
Vol. of Na2S2O3
No. of Vol. of Copper salt Mean (in
reading solution (in mL) mL) (V)
1 10
2 10
3 10
4 10
CALCULATIONS: 2 CuSO4  I2  2 Na2S2O3

1 ml 1N Na2S2O3  0.06354 gm of Cu2+
Amount of copper ions in 10 mL of copper salt solution = 0.06354 × V × S gm
Amount of copper ions in 500 mL of copper salt solution = 0.06354 × V × S × 50 gm
Observe value of Cu2+ (in 500mL solution) =
Known value of Cu2+ (in 500mL solution) =
RESULTS:
PERCENTAGE OF ERROR:
Known value − Observed value
100 =
Known value
• Why it is necessary to keep your experimental solution in the dark?
• Is it iodometric or iodimetric that you are performing?
• Tell molecular weight and gram equivalent weight of K2Cr2O7 and Na2S2O3.
• What is the function of starch?
• What is the purpose of adding NH4SCN solution?
Text: M. Mahbubul Huque and A. Jabber Mian, “Practical Chemistry”, 2 nd ed. (1972)

AMERICAN
INTERNATIONAL
UNIVERSITY –
BANGLADESH (AIUB)
EXPERIMENT NO. 7: DETERMINATION OF FERROUS ION (Fe2+) IN A SUPPLIED

SOLUTION OF IRON SALT BY STANDARD POTASSIUM DICHROMATE (K2Cr2O7)
SOLUTION.
OBJECTIVE: To know the amount of iron (Fe2+) in a supplied solution of iron salt by standard
K2Cr2O7 solution.
THEORY:
(ii) Reaction:
6FeSO4 + K2Cr2O7 + 7H2SO4 = 3Fe2(SO4)3 + K2SO4 + 7H2O + Cr2(SO4)3
Redox half reactions: 6Fe2+→ 6Fe3+ + 6e (Oxidation)
Cr2O72- + 14H+ + 6e = 2Cr3+ + 7H2O (Reduction)
(iii) Indicator: Diphenyl amine, (C6H5)2NH
APPARATUS:
REQUIRED CHEMICALS:
1. Iron salt solution,
2. 5% Sulfuric acid,
3. Conc. Phosphoric acid,
4. Standard K2Cr2O7 solution,
5. Diphenyl Amine indicator
PREPARATION OF APPROX. 0.1N POTASSIUM DICHROMATE SOLUTION.
Transfer approx. 0.49 gram of pure K2Cr2O7 into a 100 mL measuring flask and then dissolve it

Strength of K2Cr2O7 solution (S) = (N)
0.49

(Expt.7 contd.)
PROCEDURE: Take 10 mL of the supplied iron salt (Mohr’s salt) solution in a conical flask.
Add 50 mL 5% sulfuric acid and 5 mL of conc. phosphoric acid. Then add 4-5 drops of diphenyl
amine indicator and titrate slowly against the standard potassium dichromate solution drop wise
maintaining an interval of few seconds between each drop until the addition of one drop causes
the formation of intense purple or violet blue coloration which remains permanent and is
unaffected by further addition of dichromate solution. Repeat the experiment at least thrice.
Calculate the amount of iron per 500 mL of iron salt solution.
EXPERIMENTAL DATA:
Table: Determination of the amount of iron in Mohr’s salt solution using standard K2Cr2O7
solution.
Vol. of Mohr’s Vol. of K2Cr2O7
No. of Mean (in
salt solution ( in (burette reading) (in mL)
reading mL) (V)
1 10
2 10
3 10
4 10
CALCULATIONS: 1 mL 1N K2Cr2O7  0.05584 gm of Fe2+
Amount of iron in 10 mL of iron salt solution = 0.05584 × V × S gm

=
Amount of iron in 500 mLof iron salt solution = 0.05584 × V × S × 50 gm

=
Observe value of Fe2+ (in 500mL solution) =
Known value of Fe2+ (in 500mL solution) =
RESULTS:

100 =
Known value

• Why it is necessary to use both the sulfuric acid as well as phosphoric acid in the
reaction?
• Atomic weight, molecular weight of K2Cr2O7 and KMnO4.
• Could you use KMnO4 instead of K2Cr2O7?
• Why the solution shows light bottle green colour after addition of K2Cr2O7.

Attention: Please bring one graph paper

AMERICAN
INTERNATIONAL
UNIVERSITY –
BANGLADESH (AIUB)
EXPERIMENT NO. 8: DETERMINATION OF STRENGTH OF A WEAK ACID

(CH3COOH) AGAINST A STRONG ALKALI (NaOH) SOLUTION BY MEASURING
CONDUCTANCE.
OBJECTIVE:
⚫ To draw the titration curves by measuring the conductance

⚫ To find out the end-point of an acid-base reaction
⚫ To know the strength of supplied sample solution (acid or base)
THEORY:
(i) Methods: Conductometric titration,
The conductance of an acid solution varies with the amount of alkali solution added to it
because of the change in the number and nature of the ions in the solution. The curve
showing this variation in called the conductance titration curve. After complete
neutralization, the conductance increases due to the addition of the conducting ions of the
alkali. At the end-point there is a sharp change in the conductivity. Therefore, if the
conductance titration curve is drawn graphically, the end-point of the titration can easily be
determined. The conductance of a dilute acetic acid solution is due to the small amounts of
H+ ions and acetate ions resulting from the dissociation of the weak acid. When small amount
of alkali is added and the H+ ions are neutralized, an equal amount of H+ ions are not
generated by further dissociation of the weak acid because the acetate ions suppress the
dissociation due to common ion effect. Therefore the conductance decreases. Upon further
addition of alkali the conductance increases because of the addition of Na+ ions and
formation of acetate ions. After the end-point, the conductance increases at a sharper rate due
to addition of the fast moving OH¯ ions, in addition to the Na+ ions.
(ii) Reactions: CH3COOH → CH3COO¯ + H+

NaOH → Na+ + OH¯
CH3COOH + NaOH → CH3COONa + H2O
APPARATUS:
Conductivity meter, Burette (50mL), pipette (10mL), conical flask (250mL), volumetric flask
(100mL), plastic beaker, watch glass, pipette filler, dropper, glass rod, stand and clamp etc.
REQUIRED CHEMICALS:
(1) Supplied 0.1N NaOH solution, (2) Supplied dil. CH3COOH solution

(Expt.8 contd.)
PROCEDURE:
Take 10 mL of the supplied CH3COOH solution in a beaker. Place the previously washed (with
hot water) conductance cell in it and add sufficient water (~200 mL) to keep the electrodes of the
cell immersed. Measure the conductance of the acid solution (1st reading). Fill a burette with the
supplied ~ 0.1 N NaOH solution. Add 2/1 mL NaOH solution from the burette, stir the solution
and measure the conductance (2nd to 12th reading) after each addition. Get a graph paper ready
for the plotting conductance data. Plot conductance data (in s) against the final volume (in mL)
of NaOH solution on graph paper and find the end-point. The end-point gives the required
volume of NaOH equivalent to 10 ml of supplied dil. CH3COOH solution. Now calculate the
strength of CH3COOH solution.
EXPERIMENTAL DATA:
Table: Conductance-measurement of CH3COOH and NaOH solutions using conductivity meter.
Vol. of Vol. of NaOH (burette reading) (in ml.) Conductance
No. of CH3COOH Initial Final Difference Total (µs), Y
reading ( in ml.) X
1 10 0 0 0 0
2 0 2 2 2
3 2 4 2 4
4 4 6 2 6
5 6 8 2 8
6 8 10 2 10
7 10 11 1 11
8 11 12 1 12
9 12 13 1 13
10 13 14 1 14
11 14 15 1 15
12 15 16 1 16
CALCULATIONS:
Strength of supplied CH3COOH solution:

VCH3COOH x NCH3COOH = VNaOH x NNaOH
RESULTS:
(Attach the graph paper with this lab-sheet)

• Define (a) electrolyte, (b) specific conductance, (c) molar conductance
• What is conductance cell?
• How does the molar conductance of a strong electrolyte vary with concentration?
• How is the molar conductance at infinite dilution determined for (a) a strong electrolyte and (b) a
weak electrolyte?

AMERICAN
INTERNATIONAL
UNIVERSITY –
BANGLADESH (AIUB)
EXPERIMENT NO. 9: STANDARDIZATION OF POTASSIUM PERMANGANATE

(KMnO4) SOLUTION WITH STANDARD SODIUM OXALATE (Na2C2O4) SOLUTION.
OBJECTIVE: To know the strength of supplied KMnO4 solution (being made from a secondary
standard substance) by standard Na2C2O4 solution.
THEORY:
a. Method: Redox titration
b. Reaction:
2KMnO4 + 5Na2C2O4 + 8H2SO4 = 2MnSO4 + 10CO2 + 8H2O
(+ K2SO4 + 5Na2SO4)
Redox half reactions: 5C2O4 → 10CO2 + 10e (Oxidation)
2-
2MnO4- + 16H+ + 10e = 2Mn++ + 8H2O (Reduction)

c. Indicator: KMnO4 act as its own indicator.
APPARATUS:
REQUIRED CHEMICALS:
1. Sodium Oxalate,
2. 2N Sulfuric acid,
3. Potassium Permanganate
PREPARATION OF APPROX. 0.1N SODIUM OXALATE SOLUTION.
Transfer approx. 0.67 gram of pure Na2C2O4 into a 100 mL measuring flask and then dissolve it

Strength of Na2C2O4 solution = (N)
0.67

(Expt.9 contd.)
PROCEDURE:
Pipette out 10 mL standard oxalate solution in a conical flask. Add 50 mL 2N sulfuric acid
solution to it and then add 4-5 drops of KMnO4 solution from a burette. Shake the solution until
the color disappears. Continue to add permanganate solution from the burette at a moderate rate
and shake the solution continuously. As the color disappears more slowly, slow down the
addition of permanganate solution drop wise until one drop gives a light but definite pink color.
This is the end point of the titration. Repeat the process at least thrice. Take at least last reading
by heating the solution upto 60 to 700C. Calculate the mean value of burette readings and find
out the strength of supplied KMnO4 solution.
EXPERIMENTAL DATA:
Table: Standardization of supplied KMnO4 solution by standard Na2C2O4 solution.
Vol. of KMnO4
No. of Vol. of Oxalate Mean
(burette reading) (in ml.)
reading solution ( in ml.) (in ml.)
1 10
2 10
3 10
4* 10
*4th reading by heating the solution 60-700C
CALCULATIONS:
Strength of supplied KMnO4 solution:

VKMnO4 x NKMnO4 = VNa-oxalate x NNa-oxalate
RESULTS:
• Why you don’t use any indicator in this experiment?

• Molecular weight, gram equivalent weight of Na2C2O4 and KMnO4.
• Why you need to heat the experimental solution?
• What happens if you don’t shake the solution properly?

AMERICAN
INTERNATIONAL
UNIVERSITY –
BANGLADESH (AIUB)

EXPERIMENT NO. 10: DETERMINATION OF FERROUS ION (Fe2+) IN A SUPPLIED
SOLUTION OF IRON SALT BY STANDARD POTASSIUM PERMANGANATE
(KMnO4) SOLUTION.
OBJECTIVE: To know the amount of iron (Fe2+) in a supplied solution of iron salt by standard KMnO4
solution.
THEORY:
(ii) Reaction:
(1) 2KMnO4 + 5Na2C2O4 + 8H2SO4 = 2MnSO4 + 10CO2 + 8H2O (+ K2SO4 + 5Na2SO4)
Redox half reactions: 5C2O42- → 10CO2 + 10e (Oxidation)
(2) 2KMnO4 + 10FeSO4 + 8H2SO4 = 2MnSO4 + 5Fe2(SO4)3 + K2SO4 + 8H2O
Redox half reactions: 10Fe2+ → 10Fe3+ + 10e (Oxidation)
(iii) Indicator: KMnO4 act as its own indicator
APPARATUS:
REQUIRED CHEMICALS:
(1) Iron salt solution, (2) 2N Sulfuric acid, (3) KMnO4 solution and (4) Na2C2O4 solution
PROCEDURE:
PREPARATION OF APPROX. 0.1N SODIUM OXALATE SOLUTION: Transfer approx.

0.67 gram of pure Na2C2O4 into a 100 mL measuring flask and then dissolve it with distilled
water up to the mark.
Strength of Na2C2O4 solution = (N)
0.67
(A) Standardize the KMnO4 solution by standard Na2C2O4 solution: Pipette out 10 mL
standard oxalate solution in a conical flask. Add 50 mL 2N sulfuric acid solution to it and then
add 4-5 drops of KMnO4 solution from a burette. Shake the solution until the color disappears.
Continue to add permanganate solution from the burette at a moderate rate and shake the solution
continuously. As the color disappears more slowly, slow down the addition of permanganate
solution drop wise until one drop gives a light but definite pink color. This is the end point of the
titration. Repeat the process at least twice. Calculate the mean value of burette readings and find
out the strength of supplied KMnO4 solution.
Pipette out 10 mL of the iron (Mohr’s salt) solution in a conical flask. Add about 6 mL of 2N
sulfuric acid solution. Dilute to about 50 mL distilled water.
(Expt.10 contd.)
Titrate the resulting solution with standard potassium permanganate solution. The end point is
indicated by the first appearance of permanent light pink color as procedure (A). Repeat the
titration at least twice. Calculate the amount of iron per 500 mL of iron salt solution.
EXPERIMENTAL DATA:
Table-1: Standardization of supplied KMnO4 solution by standard Na2C2O4 solution.

Vol. of KMnO4
No. of Vol. of Oxalate Mean
reading solution ( in mL) (in mL)
1 10
2 10
Strength of supplied KMnO4 solution: VKMnO4 x NKMnO4 (S)= VNa-oxalate x NNa-oxalate
Table-2: Determination of the amount of iron in Mohr’s salt solution using standard KMnO4
solution.
Vol. Of Mohr’s Vol. Of KMnO4
No. Mean (in
salt solution (in (burette reading) (in mL)
of reading mL) (V)
1 10
2 10
3 10
4 10
CALCULATIONS: 1 mL 1N KMnO4  0.05584 gm of Fe2+
Amount of iron in 10 mL of iron salt solution = 0.05584 × V × S gm

=
Amount of iron in 500 mL of iron salt solution = 0.05584 × V × S × 50 gm
=
Observe value of Fe2+ (in 500mL solution) =
Known value of Fe2+ (in 500mL solution) =
RESULTS:
100 =
Known value
• Why you don’t have to use any indicator in this experiment?
• Molecular weight, gram equivalent weight of Na2C2O4 and KMnO4.
• Why you need to heat the experimental solution?
• What happens if you don’t shake the solution properly?

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AMERICAN INTERNATIONAL UNIVERSITY – BANGLADESH (AIUB)

Department of Natural Science (Chemistry)

(Quantitative Inorganic Analysis)

List of the Experiments :

1. STANDARDIZATION OF SODIUM HYDROXIDE (NaOH) SOLUTION WITH STANDARD OXALIC

2. STANDARDIZATION OF HYDROCHLORIC ACID (HCl) SOLUTION WITH STANDARD SODIUM

3. STANDARDIZATION OF HYDROCHLORIC ACID (HCl) SOLUTION WITH STANDARD SODIUM

4. DETERMINATION OF TOTAL HARDNESS OF WATER USING ERIOCHROME BLACK T (EBT) AS

5. STANDARDIZATION OF SODIUM THIOSULPHATE (Na2S2O3.5H2O) SOLUTION WITH STANDARD

6. ESTIMATION OF COPPER CONTAINED IN A SUPPLIED SOLUTION BY IODOMETRIC METHOD.

7. DETERMINATION OF FERROUS ION (FE2+) IN A SUPPLIED SOLUTION OF IRON SALT BY

8. DETERMINATION OF STRENGTH OF A WEAK ACID (CH3COOH) AGAINST A STRONG ALKALI

9. STANDARDIZATION OF POTASSIUM PERMANGANATE (KMnO4) SOLUTION WITH STANDARD

10. DETERMINATION OF FERROUS IONS (FE2+) IN A SUPPLIED SOLUTION OF IRON SALT BY

CHEM 1101: CHEMISTRY (EEE/CoE/CSE/IPE)

EXPERIMENT NO. 1: STANDARDIZATION OF SODIUM HYDROXIDE (NaOH)

PREPARATION OF APPROX. 0.1N OXALIC ACID SOLUTION. Transfer approx. 0.63

Weight taken (in gm)  0.1

PROCEDURE: Take 10 mL of NaOH solution in a conical flask by means of a pipette and

May, 2022 Chemistry Lab Sheet

Strength of supplied NaOH solution:

VNaOH x NNaOH = VOxalic acid x NOxalic acid

Students should know

May, 2022 Chemistry Lab Sheet

CHEM 1101: CHEMISTRY (EEE/CoE/CSE/IPE)

EXPERIMENT NO. 2: STANDARDIZATION OF HYDROCHLORIC ACID (HCl)

(A) Standardize the supplied NaOH solution as in Experiment No. 1

Weight taken (in gm)  0.1

Strength of supplied NaOH solution:

May, 2022 Chemistry Lab Sheet

(B) Preparation of approximately 0.1N hydrochloric acid solution:

PROCEDURE: Take 10 mL of NaOH solution in a conical flask by means of a pipette and

(A) Strength of supplied dil. HCl solution:

(B) Strength of conc. HCl solution:

Students should know

May, 2022 Chemistry Lab Sheet

CHEM 1101: CHEMISTRY (EEE/CoE/CSE/IPE)

EXPERIMENT NO. 3: STANDARDIZATION OF HYDROCHLORIC ACID (HCl)

PREPARATION OF APPROX. 0.1N Na2CO3 SOLUTION: Transfer approx. 0.53 gm of

May, 2022 Chemistry Lab Sheet

Vol. of HCl (in mL)

(A) Strength of supplied dil. HCl solution:

(B) Strength of conc. HCl solution:

Student should know:

May, 2022 Chemistry Lab Sheet

CHEM 1101: CHEMISTRY (EEE/CoE/CSE/IPE)

EXPERIMENT NO. 4: DETERMINATION OF TOTAL HARDNESS OF WATER

OBJECTIVE: To determination of total Hardness of water using Eriochrome Black T (EBT) as

Ethylenediaminetetraacetic acid (EDTA) form a chelated soluble complex when added to a

(ii) Reaction: (Y= deprotonated part of EDTA, In=EBT Indicator)

Titration reaction: HY3–(aq) + Ca2+(aq) → CaY2–(aq) + H+(aq) (also for Mg2+)

End point reaction: HY3–(aq) + MgIn–(aq) → MgY2–(aq) + HIn2–(aq)

(iii) Indicator: Eriochrome Black T (EBT), a complexometric indicator.

Strength of EDTA solution = (M)

Table-2: Determination of total Hardness of water using Eriochrome Black T (EBT) as

1 ml 0.01 M EDTA solution  1.00 mg of CaCO3 (as shown below)

[where, a = ml. of titrant required (mean volume of EDTA solution)

Students should know

May, 2022 Chemistry Lab Sheet