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    Plant Tissue Culture-Introduction

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    nehabano336

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    Plant Tissue Culture-Introduction

    Uploaded by

    nehabano336
    4 views11 pages

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    4 views11 pages

    Plant Tissue Culture-Introduction

    Uploaded by

    nehabano336

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 11

    Dr.

    Shefali Singh
    Assistant Professor
    K. N. Govt. PG College,
    Gyanpur
    Tissue culture
    • Tissue culture is the method of
    growing cells artificially in the
    laboratory conditions.
    • It refers to growing plants under in
    vitro conditions on nutrient media
    and aseptic conditions.
    • Plant tissue culture techniques is
    widely used produced large no. of
    clones (micropropagation).
    • Plant tissue culture relies on the
    ability of totipotency (the term was
    given by Haberlandt).
    • Totipotency is the genetic potential
    of a plant cell to produce the entire
    plant.
    • Haberlandt was the first scientist to produce whole plants from
    plant tissues and so he is popularly called as the “Father of Tissue
    Culture’. Plant tissue culture and molecular biology form the basis
    for genetic engineering.
    Culture media
    Essential Macro-elements, microelements, iron source
    elements

    Carbon Example : Sucrose


    source

    Organic
    supplement Vitamins, aminoacids
    s

    Solidifying Example: Agar media


    agent

    Growth phytohormones etc.


    regulators
    • The plant hormones auxin and
    cytokinin are critical for plant
    regeneration in tissue culture, with
    cytokinin playing an instrumental
    role in shoot organogenesis.
    • Auxin/cytokinin ratio (Skoog and
    Miller)
    Advantages of Tissue culture
    • Plant tissue culture technology is being widely used for large scale plant
    multiplication.
    • Plant tissue culture techniques have become of major industrial importance in the
    area of plant propagation, disease elimination, plant improvement, and
    production of secondary metabolites.
    • Small pieces of tissue (named explants) can be used to produce hundreds and
    thousands of plants in a continuous process. A single explant can be multiplied
    into several thousand plants in a relatively short time period and space under
    controlled conditions, irrespective of the season and weather on a year-round
    basis.
    • Endangered, threatened, and rare species have successfully been grown and
    conserved by micropropagation because of the high coefficient of multiplication
    and small demands on the number of initial plants and space.
    • In addition, plant tissue culture is considered to be the most efficient technology
    for crop improvement by the production of somaclonal and gametoclonal variants.
    • The micropropagation technology has a vast potential to produce plants of
    superior quality, isolation of useful variants in well-adapted high yielding
    genotypes with better disease resistance and stress tolerance capacities

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