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Aim
To study budding in Hydra with the help of prepared slides.
Theory Hydra is exclusively a freshwater organism having different species. It is very small, just a half centimetre long. It is a cnidarian having a tubular body which is composed of a head, distal end and afoot at the end. For reproduction, Hydra uses regenerative cells where a bud expands as an outgrowth because of repeated cell division at one specific location. These buds then developed into new small individuals which when completely matured, detach from the parent body. Materials required Prepared slides of Hydra showing budding with different stages. Compound microscopes. Procedure: 1. Take the prepared slide of budding in Hydra and place it on the stage of the compound microscope. 2. Adjust the mirror of the microscope so that maximum light is reflected from the mirror on to the slide. 3. Focus the slide under the microscope’s lens carefully, first under low power and then under high power. 4. Observe the different phases of budding in Hydra through the lens of the compound microscope Observations 1. Hydra is vase shaped. Tentacles can be seen at the open end. 2. It is a multicellular organism. 3. A bud can be seen protruding out from the parent organism. 4. Different stages of budding can be observed in the slide. 5. A fully formed detached organism can also be observed. Conclusion he given slides showed the small growth (bud) on Hydra. These buds on maturity T separate from the parent cell and grow as new organisms, hence, Hydra shows budding. Precautions: ● Before placing the slide on the stage of the microscope, ensure the settings of the microscope mirror and diaphragm are correct. ● Focus the slide under the microscope’s lens carefully, first under low power and then under high power.