English Aerospray Stat (7122) Applications Manual 57-2000-01A

AEROSPRAY® HEMATOLOGY STAT

SLIDE
STAINER/CYTOCENTRIFUGE

Model 7122

Applications Manual

57-‐2000-‐01A

©2013 ELITechGroup Inc. All rights reserved. Printed in the United States of America.
No part of this publication may be reproduced, transmitted, transcribed, stored in a
retrieval system, or translated into any language (human or computer) in any form, or
by any means whatsoever, without the prior express written permission of
ELITechGroup Inc.

Wescor, Aerospray and Cytopro are trademarks of ELITechGroup Inc.

Other trade names used in this manual are trademarks of their respective owners, used
here for information only.

ELITechGroup Inc. makes no express or implied warranty regarding this manual, its
quality, performance, or appropriate use regarding any type of specific procedure.
ELITechGroup Inc. may modify this manual without notice and without implying any
obligation or liability on the part of the company.

Manufactured in the United States of America by:

ELITechGroup Inc.
370 West 1700 South
Logan, Utah 84321-‐8212 USA

2
Table of Contents

Section 1
Introduction
1.1 Instrument Overview ...................................................................... 7
Using this Manual .................................................................. 7
Functional Description .......................................................... 8
Key Features .......................................................................... 9
Intended Use ......................................................................... 9
Table 1: General Specifications ............................................. 10
Table 2: Performance Specifications ..................................... 11
Table 3: Run Time Sequence .................................................. 12
Table 4: Approximate Reagent Consumption ........................ 12
Table 5: Carousel and Rotor Information .............................. 13
Table 6: Explanation of Symbols ............................................ 14
1.2 Instrument Description ................................................................... 16
Figure 1: Front and Right Side Panels .................................... 16
Figure 2: Front Panel and Touchscreen ................................. 17
Figure 3: Rear Panel .............................................................. 17
Figure 4: Stainer Bowl Components ...................................... 18
Figure 5: Nozzle Diagram ...................................................... 18
Table 7: Preventive Maintenance Kit ..................................... 19
Barcode Reader ..................................................................... 20
Figure 6: Barcode Reader ...................................................... 20
1.3 Touchscreen and User Interface ..................................................... 21
Table 8: Front Panel/Main Screen Function Keys .................. 21
Table 9: System Setup Keys ................................................... 22
Table 10: Maintenance Function Keys ................................... 24

Section 2
Setup and Preparation for Operation
2.1 Instrument Setup ............................................................................ 25
Unpacking and Installing the Stainer ..................................... 25
Connecting the Drain Tube and Waste Container ................. 26
Connecting Power ................................................................. 26
Installing Reagent Bottles ...................................................... 27
Installing the Barcode Reader ............................................... 28
2.2 Preparing the Stainer for Operation ............................................... 29
Priming Procedures ............................................................... 29
The Clean Cycle ..................................................................... 31
Performing Tests ................................................................... 31
Reagent Level Monitoring ..................................................... 32
Enabling/Disabling Reagent Level Detect ........................ 32
Zeroing the Reagent Level Sensors .................................. 33

Section 3
Controlling and Customizing Stainer Functions
3.1 System Setup Menu ........................................................................ 35
Accessing the System Setup Menu ........................................ 35
Stain Programs ...................................................................... 35
Creating a Stain Program ...................................................... 36
Editing, Renaming, or Adjusting Stain Programs ................... 36
Administrator and User Accounts ......................................... 37
Creating an Administrator Account ................................. 37
3
Table of Contents

Section 3
Controlling and Customizing Stainer Functions (continued)
Creating User Accounts ................................................... 38
Managing User Access .................................................... 39
User Login/Logout ........................................................... 39
Using Reagent Information Tracking ..................................... 41
Modifying Level Detect Functions ......................................... 41
Changing User Language ....................................................... 42
Setting the Date and Time ..................................................... 42
System Log ............................................................................ 42
Accessing Logs ................................................................. 42
Exporting Logs ................................................................. 43
Controlling Beeper Alerts ...................................................... 43
QC/Maintenance Tracking .................................................... 44
Enable Stain Slide Tracking .............................................. 44
Enable Cyto Slide Tracking ............................................... 45
Enable Manual Entry ....................................................... 45
Enable Preventive Maintenance Tracking ....................... 45
Enable Reagent Tracking ................................................. 45
Restoring Software Defaults ................................................. 46
3.2 Recording Specimen and Reagent Information .............................. 47
Scanning Slides with the Barcode Reader ............................. 47
Scanning Reagent Bottles with the Barcode Reader ............. 48
Manually Entering Specimen information ............................ 49
Manually Entering Reagent Information ............................... 50
3.3 The Help Menu ................................................................................ 51
Using Help ............................................................................. 51

Section 4
Operating the Stainer
4.1 Operating Instructions .................................................................... 52
Suggested Staining Protocol .................................................. 52
Performing a Hub Pattern Test ............................................. 52
Figure 7: Correct Hub Pattern Test Result ..................... 52
Figure 8: Incorrect Hub Pattern Test Result ................... 52
Loading the Carousel ............................................................. 53
Figure 9: Loading the Carousel ...................................... 54
Figure 10: Specimen Placement on Slide ....................... 54
Using Blocking Slides ............................................................. 55
Figure 11: Using Blocking Slides .................................... 55
Performing a Stain Cycle ....................................................... 55
Unloading the Carousel ......................................................... 56
Monitoring Reagent and Waste Levels ................................. 57
Table 11: Reagent Level Detect Display Symbols .......... 57
Replacing a Reagent Bottle ................................................... 58
Emptying the Waste Container ............................................. 58
Section 5
Preventive Maintenance and Safety
5.1 Preventive Maintenance ................................................................. 59
Daily Maintenance/Quality Control (QC) .............................. 59
Weekly Maintenance ............................................................ 59
Monthly Maintenance ........................................................... 60
Using the Preventive Maintenance Log ................................ 60

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Table of Contents

Section 5
Preventive Maintenance and Safety (continued)
5.2 Storing the Instrument .................................................................... 61
Preparing for Long-‐Term Storage .......................................... 61
5.3 Replacing Fuses ............................................................................... 62
5.4 Cleaning the Stainer and Carousels ................................................. 63
Cleaning Liquid Spills ............................................................. 63
Cleaning Broken Slides .......................................................... 63
5.5 Decontaminating the Stainer and Carousels ................................... 64
Solutions for Decontaminating the Instrument .................... 64
Figure 12: Lid Latch and Locking Pin Hole Locations ..... 65
Decontaminating the Instrument .......................................... 65
Decontaminating Reagent Bottles ........................................ 66
5.6 Shipping or Disposing of the Stainer or Carousels .......................... 67
Shipping the Instrument or Carousels ................................... 67
Hazard-‐Free Certification .............................................. 67
Disposing of the Instrument or Carousels ............................. 67

Section 6
Nozzle Maintenance and Performance
6.1 Nozzle Disassembly and Cleaning ................................................... 68
Nozzle Disassembly ............................................................... 68
Nozzle Cleaning ..................................................................... 68
6.2 Nozzle Reassembly .......................................................................... 69
6.3 Manual Priming ............................................................................... 70
6.4 Performing the Volume Test ........................................................... 71
Table 12: Volume Test Tolerances ......................................... 71
6.5 Performing the Slide Pattern Test ................................................... 73
Figure 13: Correct Slide Pattern Test Result .......................... 73
Figure 14: Incorrect Slide Pattern Test Result ........................ 73

Section 7
Solving Problems
7.1 Troubleshooting .............................................................................. 74
Table 13: General Troubleshooting and Diagnosis ................ 74
7.2 Abnormal Staining Results .............................................................. 80
Smear Separation or Tearing ................................................. 80
Improper Alcohol Fixation ................................................. 80
Wet Smears ....................................................................... 80
Thick Smears ...................................................................... 80
Dirty Slides ......................................................................... 80
Red Cell Artifacts ............................................................... 80
Use Aerofix® ...................................................................... 81
False-‐Positive Howell-‐Jolley Bodies or Amorphous
Precipitates ........................................................................ 81
7.3 Instrument Malfunction .................................................................. 83
Air or Reagent Leaks .............................................................. 83
Reagent Level Detect System Errors ..................................... 84
Calibrating the Reagent Level Detect System ....................... 84
7.4 Calibrating the Touchscreen ........................................................... 86
7.5 Service Information ......................................................................... 87

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Table of Contents

Section 8
Cytopro® Cytocentrifuge
8.1 Cytopro Cytocentrifuge Information ............................................... 88
Functional Description .......................................................... 88
Key Features .......................................................................... 88
Intended Use ......................................................................... 88

Appendix A: Critical Reagent Components ............................................ 89

Appendix B: Risk and Safety Phrases ..................................................... 91

Appendix C: Accessories and Supplies .......................................................... 94

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INTRODUCTION

1.1 Instrument Overview
Using this Manual
This manual provides instructions to install, operate, and maintain the Aerospray
Hematology Stat Stainer/Cytocentrifuge. The manual is an important part of the
product. Read it carefully and completely before setup and first use of the instrument.

If additional accident prevention and environmental protection requirements exist in
the country of operation, this manual must be supplemented by appropriate
instructions to ensure compliance.

Safety Regulations
This instrument has been built and tested in accordance with safety regulations for the
following device types: electrical control, regulating, and laboratory. In order to
maintain this condition and ensure safe operation, the operator must observe all the
instructions and warnings contained in this manual. For current information about
applicable standards, please refer to the CE Declaration of Conformity included with the
documents shipped with this device.
NOTE: This equipment complies with the emission and immunity requirements described
in the IEC 61326 series.
Understanding Warnings
This manual uses three warning levels to alert you to important information as shown in
the following examples.

WARNING!
A Warning alerts to the possibility of personal injury, death, or other serious adverse
reactions stemming from the use or misuse of this device or its components.

CAUTION:
A Caution alerts to possible problems with the device associated with its use or misuse.
Such problems include device malfunction, failure, damage, damage to the sample, or
damage to other property. Where applicable, a Caution may include precautions to be
taken to avoid the hazard.

NOTE: A Note reinforces or supplies additional information about a topic.

Specific Warnings
Pay particular attention to the following safety precautions. If these safety precautions
are ignored, injury or damage to the instrument may occur. Each individual precaution is
important.

WARNING!
Install the stainer in a well-‐ventilated area. If ventilation is inadequate, operate the
instrument under a safety hood.
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SECTION 1
INTRODUCTION

WARNING!
Reagents used in the stainer contain moderately hazardous chemicals that require
care in handling. Always use appropriate safety measures including gloves and eye
protection when handling reagents.

WARNING!
Always wear protective clothing and eye protection when using Nozzle Cleaning
Solution (diluted REF: SS-‐029C). Dispose of used solution properly.

WARNING!
If power is lost while the stainer is running, the lid will remain locked until power is
restored. Do not attempt to open the lid while power is off.

WARNING!
Electrical shock hazard: Do not open this instrument or attempt internal repairs. Refer
servicing to qualified service personnel. Contact Elitech Group Biomedical Systems
service.

CAUTION:
This equipment has been designed and tested to CISPR 11 Class A and FCC Part 15 Class
A. In a domestic environment it may cause radio interference, in which case, you may
need to take measures to mitigate the interference.
CAUTION:
To avoid serious instrument damage, always use reagents supplied by Elitech Group.
Except for approved methanol, using reagents not supplied by Elitech Group may void
the warranty.

CAUTION:
Only spare parts supplied or specified by Elitech Group should be used in this device.
Using non-‐approved parts may affect the performance and safety features of the
instrument. If the equipment is used in a manner not specified by Elitech Group the
protection provided by the equipment may be impaired. If in doubt, contact your Elitech
Group representative.

Functional Description
The Aerospray Hematology Stat Slide Stainer/Cytocentrifuge (Model 7122) is a dual-‐
purpose, microprocessor-‐controlled slide staining and cell preparation system. In use,
atomizing spray nozzles apply fresh reagents onto microscope slides prepared with
blood or other body fluid specimens. The slides are mounted in a rotating carousel for
processing.

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INTRODUCTION

Key Features
• Minimized reagent consumption
• Rapid staining
• Barcode scanner for tracking specimens and reagents
• Reagent and specimen traceability
• User traceability
• Administrator password
• Interactive touchscreen display
• Multiple languages
• High-‐volume staining productivity (12 slides per stain cycle)
• Separate reservoir, delivery tube, pump, and spray nozzle for each reagent
• Operator-‐selectable automatic alcohol fixation function to fix specimens
• Reagent and waste level monitoring
• Log files
• 10 Stain Intensity Settings
The correct accessory must be used for each function. The Cytopro Cytocentrifuge Rotor is
available as an option offering additional features (see Section 8).

Intended Use

The Aerospray Hematology Stat Slide Stainer/Cytocentrifuge (Model 7122) is intended for
use by medical professionals to stain specimens that may include blood and other body
fluids, as a step of standard laboratory practice in diagnosing disease in humans. Addition of
the Cytopro rotor allows preparation of slides by cytocentrifugation before staining.

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INTRODUCTION

Table 1: General Specifications
Category Characteristics
Slide Carousel Capacity 1 to 12
Carousel Rotation Speed Reagent application: 20 rpm (± 5%)
Slide Drying: 950 rpm (± 5%)

Cytocentrifuge Rotor Speed 100 to 2000 rpm (± 5%), user programmable

Reagent Consumption Refer to Approximate Reagent Consumption, Table 4

Operating Time Refer to Run Time Sequence, Table 3

Display 7 in. WVGA (800 x 480 pixels) TFT

Touchscreen Controls Menu-‐driven icons

Drain Connection Connector on rear panel accepts male connector
attached to vinyl drain tube. 1.8 meters (6 ft.) length
supplied

Ventilation Air is exhausted from the stainer via a female ½ inch SAE
pipe thread fitting
Dimensions
Width 57 cm (22 in.)
Height (lid closed) 25 cm (10 in.)
Depth 54 cm (21 in.)
Height (lid open) 58 cm (23 in.)

Weight 15.2 kg (33.5 lb.)

Electrical Requirements 100 to 240 VAC (± 10%) @ 50 to 60 Hz

Power Consumption 200 Watts maximum

Fuses 2.0 Amp time-‐lag (Type T) (5 x 20 mm) (2 required)
Manufactured by Littlefuse (Part No. 218002) or
Bussman (Part No. GDC-‐2A)

Ambient Temperature
Operating 15 to 30 °C (59 to 86 °F)
Storage -‐10 to 50 °C (14 to 122 °F)

Relative Humidity ≤ 80% non-‐condensing

Altitude ≤ 2000 m (≤ 6562 ft.)

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INTRODUCTION

Table 1: General Specifications (continued)

Pollution Degree 2

Heat Dissipation
Maximum 512 Btu/hour max
Average During Staining 34 Btu/hour
Average While Idle 10 Btu/hour

Maximum Sound Emission 72 dB

Table 2: Performance Specifications
Reagent Spray Nozzles Four reagent nozzles: A, B, C, D
Reagents: A -‐ Rinse

NOTE: Use only Elitech Group reagents, with diluents B – Thiazin Stain (Blue)
as specified for Elitech Group concentrated reagents.
REF numbers for this stainer start with one of the C – Eosin Stain (Red)
following: SS-‐035, SS-‐049, SS-‐135, SS-‐149, SS-‐048, or
SS-‐148. D – Methanol or Aerofix®

Note: Methanol must be 99.5% pure no more than 0.5%
water.

Stain Settings Number of slides to stain. Intensity settings: 1-‐10.
Fixation level: Off, Normal, High

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INTRODUCTION

Table 3: Run Time Sequence
NOTE: Table 3 represents a typical timing sequence for this instrument using the 12-‐slide
carousel, presented for general reference only. Timing is in seconds. Actual cycle times may vary.

Staining Mode Medium Dark Setting (5)
Fixation Pump D (0ptional) (102)
Eosin Stain (Pump C) 21
Thiazin Stain (Pump B) 33
Rinse (Pump A) 27
High Speed Dry 33

Total without fixation (in minutes) 1.9
Total with fixation (in minutes) 3.6

Table 4: Approximate Reagent Consumption (mL )

Staining Mode Medium Dark Clean Cycle
Setting (5)
Reagent A: Rinse 6.0 7.5
Reagent B: Thiazin 6.0 7.5
Reagent C: Eosin 6.0 7.5
Reagent D: Fixative 1.4 38.0
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INTRODUCTION
Table 5: Carousel and Rotor Information
Only the following slide staining carousel or cytocentrifuge rotor can be used in this instrument.
Each should be used following the instructions in this manual or the Cytopro Applications Manual
(RP-‐517).
Rotor/Carousel Maximum Maximum Capacity Maximum
rpm Sample
Volume
12-‐Slide Carousel (AC-‐028) 1000 rpm 12 each, 26 mm x 76 mm N/A
(1 x 3 inch) microscope
slides
Cytopro Cytocentrifuge Rotor (AC-‐160) 2000 rpm 8 each, standard Up to 600 µL*
chambers, plus slides

8 each, Cytopro Magnum Up to 6 mL*
chambers, plus slides

*Do not overfill cytocentrifuge chambers. See Cytopro Applications Manual or Methods Manual
for detailed instructions and warnings.

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INTRODUCTION

Table 6: Explanation of Symbols

Alternating Current (AC)

Authorized Representative in the European Community

Batch Code

Biological Hazards (Biological Risks)

Catalog Number (Model Number)

Caution, Consult Accompanying Documents (Attention, see instructions for use)

CE Mark, product meets the essential requirements designated in Annex I of the
In Vitro Device Directive (IVDD) 98/79/EC

Consult Instructions For Use

Do Not Reuse

Do not use if package is damaged

Fragile, Handle with Care

Fuse

General Symbol for Recovery, Recyclable

In vitro Diagnostic Device (In vitro Diagnostic Medical Device)

Keep away from sunlight (Keep away from heat)

Manufacturer

Waste Electrical and Electronic Equipment

Reagent stable after opening for number of months specified

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INTRODUCTION

Table 6: Explanation of Symbols (continued)
I “On” (Power)

O “Off” (Power)

Environment Friendly Use Period

Serial Number

Temperature Limitation – indicates high and low limits. (Normal room
temperatures are specified for all Elitech Group Reagents.)

Use By

Volume Control

General Warning, Caution, Risk of Danger

Warning, Biological Hazard

Corrosive

Environment Hazard

Flammable

Harmful/Irritant

Oxidant

Toxic

Product to be used for manual cleaning only. Do not pump product through

instrument.

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INTRODUCTION

1.2 Instrument Description
Figure 1: Front and Right Side Panels

4

3

2
5
1
D
C
B
A

1 – Front Panel with Touch Screen Display 6
2 – Bowl
3 – Carousel
4 – Lid with Safety Lock
5 – Right Side Panel with Label Indicating Reagent Positions:
A – Rinse
B – Thiazin
C – Eosin
D – Methanol or Aerofix
6 – Reagent Tray
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INTRODUCTION

Figure 2: Front Panel and Touchscreen

1

2

1 – Standby/Ready Button
2 – Touchscreen

The front panel features an interactive touchscreen display. Refer to Front Panel/Main
Screen Function Keys (Section 1.3, Table 8) for more information.

Figure 3: Rear Panel 1 1 2 3

4

5

11 10 9 8 7 6
1 – USB Ports
2 – Network Ethernet Connection
3 – Exhaust Vent
4 – Rear Panel Label
5 – Biohazard Warning Label
6 –Waste Tube Connection
7 – Level Detection Connection for Waste Container
8 – Power Switch
9 – Fuse Door
10 – Power Cord Connection
11 – Model/Serial Number Label
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SECTION 1
INTRODUCTION

Figure 4: Stainer Bowl Components

1 2 3 4

5

1 – Nozzle D (Methanol or Aerofix)
2 – Nozzle C (Eosin stain)
3 – Nozzle B (Thiazin stain)
4 – Nozzle A (Rinse)
5 – Drive Hub

Figure 5: Nozzle Diagram

1 2 3 4

1 – Compression Screw
2 – Swirl Cone
3 – O-‐Ring
4 – Nozzle Housing
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SECTION 1
INTRODUCTION

Table 7: Preventive Maintenance Kit

Component Name Description
Manual Priming Tool Primes air-‐locked pumps.

Silicon Grease Lubricates the nozzle threads for
easy assembly.

Nozzle Wire Cleans nozzle housing orifices.

Nozzle Cleaning Strainer Strains the nozzle parts to prevent
them from going down a drain.

Nozzle Tool Unscrews nozzles from the stainer

bowl.

Nozzle Wrench Disassembles the nozzle.

Nozzle Brush Cleans nozzles without removing

them from the stainer.

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SECTION 1
INTRODUCTION

Table 7: Preventive Maintenance Kit (continued)

Component Name Description
Volume Test Collection Collects reagents while performing
Tubes (small tube) the Volume Test.

Nozzle Maintenance Tube Holds Nozzle Cleaning Tubes (large
Stand tube) and Volume Test Tubes (small
tube).

Nozzle Cleaning Tubes For soaking nozzles in the Nozzle
(large tube) Cleaning Solution.

Barcode Reader
An optional barcode reader is available for the Hematology Stat Stainer/Cytocentrifuge
(Model 7122).

Figure 6: Barcode Reader

20
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INTRODUCTION

1.3 Touchscreen and User Interface
Users control all instrument functions from the interactive touchscreen display.

Table 8: Front Panel/Main Screen Function Keys

Button Name Description
Standby/Ready With instrument power ON:
Blue = Ready
Amber = Standby

Pressing Standby runs a System Clean cycle and
places instrument into standby mode.

The Standby/Ready button also accesses the
touchscreen calibration function. Refer to
System Setup Menu (Section 3.1).

Maintenance Accesses features for verifying proper nozzle
performance and places pumps in a testing
sequence. Accesses the line priming, Pattern
Test, Volume Test, and B-‐Line Flush functions.

Clean Performs the Clean cycle.

Cyto Enters the Cytocentrifuge mode.

System Information Shows the system information, including serial
number and software version. Allows access to
the System Setup features. Refer to System
Setup Menu (Section 3.1).

Help Opens the software Help file.

Programs Allows users to select or edit programs.

Start/Load Slides Starts a Stain or Cytocentrifuge cycle. Button is
inactive until a program is created. Refer to
Creating a Stain Program (Section 3.1).

With Slide Tracking enabled, opens the Scan
and Load Slides menu (Section 3.2).

Number of Specimen Selects the number of specimen slides in the
Slides carousel. Users staining an odd number of
specimen slides should press the next higher
specimen slide number icon.

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SECTION 1
INTRODUCTION

Table 8: Front Panel/Main Screen Function Keys (continued)

Back Returns to the previous menu.

Stop Aborts any operation.

OK Indicates completion of current task.
System Setup Allows users to modify the software settings.

See System Setup Menu (Section 3.1).

Table 9: System Setup Keys

Stain Programs Allows users to create, edit, and delete stain
programs.

Cyto Programs Allows users to create, edit, and delete
cytocentrifuge programs.

Reagents Allows users to edit reagent information.

Users Allows users to create and change user
accounts.

QC/Maintenance Enables slide tracking, preventive maintenance
Tracking tracking, and reagent tracking.

Level Detect Allows users to manage the automatic reagent
level detection system.

Language Allows users to change the display language.

System Log Allows users to control logging functions.

Network Settings Allows users to change network settings.

Beeper Allows users to change audible alerts.

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SECTION 1
INTRODUCTION

Table 9: System Setup Keys (continued)

Set Date/Time Allows users to set the date and time.

Restore Defaults Restores programming to default settings.

Login Enters Login sequence for authorized users.

Logout Logs authorized users out. Users must log in
again to use the stainer.

Save Saves the entered or selected information.

Add Enters programming mode for creating staining
and cytocentrifuge programs. Also allows the
system administrator to authorize new users.
Allows manual entry of slide or specimen
information.

Delete/Erase/Remove Deletes or erases the selected item.

Edit/Change User Allows editing of an existing stain or
cytocentrifuge program. Allows manual entry of
slide or specimen information (stain or
cytocentrifuge mode). Also allows system
administrator to edit user information.

Zero Zeros the Level Detect sensors.

Calibrate Calibrates the Level Detect system.

Unselected Shows an unselected option.

Selected Shows a selected or enabled option.

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SECTION 1
INTRODUCTION

Table 10: Maintenance Function Keys

Individual Prime Primes the selected line.
Buttons (A, B, C, D)

Pattern Test Performs Pattern Test to ensure nozzles are
clear of debris and spraying properly.

Volume Test Performs Volume Test to verify the selected
nozzle volume is within the correct range.

60-‐Sec Prime Runs the pumps for 1 minute and primes the
lines.

QC/PM Shows the Preventive Maintenance and Quality
Control logs, when enabled from the System
Setup menu (Section 3.1).
Line Flush Cleans the B and C reagent lines.

ABCD Prime button Primes all (A, B, C, D) reagent lines
simultaneously.
24
SECTION 2
SETUP AND PREPARATION FOR OPERATION

2.1 Instrument Setup
Unpacking and Installing the Stainer
Follow this sequence if you are using this instrument for the first time. Details about
these operations are given in the next three sections.
CAUTION:
Contact Elitech Group before installing the instrument if you observe any damage to the
packaging or equipment.

1 Unpack and inspect the instrument.

2 Check that the contents of the boxes match the packing lists for the
instrument and accessories.

3 Open the instrument lid and remove the cardboard tube that protects the
hub.

NOTE: Keep the box and packaging material to repack the instrument if you
intend to ship it to the manufacturer for service.

4 Place the instrument on a flat surface, free from dust and vibration and
away from direct sunlight.

NOTE: Position the instrument with the rear panel at least 30 cm (12 in.) from
obstructions or hazardous materials.

CAUTION:
Keep the drain tube straight and as short as possible. The maximum length is
1.8 m (72 in.). The waste container must be positioned lower than the stainer.

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SECTION 2

Connecting the Drain Tube and Waste Container
1 Insert the waste tube connector into the rear panel receptacle until you
hear a click.

2 Adjust the tube length to less than 1.8 m (72 in.).
NOTE: Ensure the waste tube has no loops or kinks, and is as straight and as
short as possible. Cut off excess tubing as needed.

3 Connect the drain tube to the waste container.

If using a waste bottle with level detect (REF: AC-‐182):
4 Connect the waste monitoring cable to the rear panel receptacle.

5 Connect the waste monitoring cable to the waste container lid.

Connecting Power
1 Make sure the power switch is OFF (O).

2 Plug the power cord into the power connector on the rear panel of the
instrument.
NOTE: Use a surge protector to isolate the instrument from electrical spikes and
surges.

3 Plug the power cord into a properly rated AC electrical outlet.

4 Turn the power switch ON (I). After a brief delay the Main menu will
appear.

26
SECTION 2

Installing Reagent Bottles
WARNING!
Reagents used in the instrument contain moderately hazardous chemicals that require
care in handling. Always handle reagents using appropriate safety measures, including
gloves and eye protection.
NOTE: Reagents should be stored according to the conditions specified on their label.
After opening, reagents are stable until the expiration date specified on the label unless
otherwise indicated by the symbol shown at left.
1 Place each 500 mL reagent bottle in the correct position.
(A) Rinse
(B) Thiazin Stain
(C) Eosin Stain
(D) Methanol
NOTE: See Appendix A for complete identification of all reagents used in this
A B C D stainer.

CAUTION:
To avoid severe damage, never use reagents containing organic solvents (such
as ketones) in this instrument, unless supplied by Elitech Group, or specified in
official Elitech Group formulation instructions.

NOTE: Immediately remove spills in the reagent tray to preserve the accuracy of
the reagent level detecting system.

2 For all reagents:
• Open a new bottle of reagent.
• Record the reagent letter on each cap.
• Insert the corresponding dip tube into the reagent bottle and install
the ring cap.

27
SECTION 2

Installing the Barcode Reader
A barcode reader can be connected to the stainer for scanning reagent bottles and
specimen slides that contain barcodes. This allows easy reagent and specimen
information tracking. If a barcode reader is not installed, reagent and specimen
information can be entered manually (Section 3.2).

Installing the Barcode Reader
1 Place the barcode reader and stand on a level surface near the stainer.

2 Plug the barcode reader into the left USB port on the rear panel of the
stainer. See Section 3.2 for instructions on using the barcode reader.

28
SECTION 2

2.2 Preparing the Stainer for Operation
Priming Procedures
NOTE: The instrument is shipped with alcohol in the reagent lines. For proper
performance, this alcohol must be replaced with the correct reagent for each reagent
line prior to use.
Thoroughly purge and prime each reagent delivery line using the following instructions.
1 Remove each spray nozzle with the provided nozzle tool by turning
counterclockwise.

D C B A 2 Note the location of each nozzle so you can return it to the original
position during reassembly.

3 Place a carousel on the stainer hub to prevent stain from entering the
motor shaft.

CAUTION:
Fluid from priming can flood and damage the motor if the drain tube is not
properly installed.

4 Press Maintenance from the Main menu.

5 Press the A prime button. Stain should appear within 10 seconds. When
properly primed, a steady stream of reagent (no sputtering or breaks)
flows from the nozzle receptacle.

• If stain appears, proceed to the next step.

• If stain does not appear within 10 seconds, perform the manual
priming procedure (Section 6.3).
CAUTION:
Never operate a dry pump for more than 10 seconds. Operating a dry pump
may cause damage to the instrument.

29
SECTION 2

Priming Procedures (continued)
6 Repeat the previous steps for each reagent line (B, C, and D).

7 Press 60-‐Sec Prime to prime each reagent line with 200 mL of reagent to
remove all of the alcohol from the reagent lines and pumps.

8 To prime all lines at the same time, press ABCD. The pumps will run for 1
minute and prime all the lines. Follow instructions on the screen.

NOTE: To prime individual lines, press the appropriate individual prime button
(A, B, C, D).

D C B A
9 Return the nozzles to their original positions and tighten clockwise with
the nozzle tool.

10 With the nozzles installed, repeat Steps 5 and 6. A fine cone of spray
should come from each nozzle.

11 After verifying nozzle performance, a clean cycle must be performed
before a stain cycle can be run.
30
SECTION 2

The Clean Cycle
The Clean cycle is a two-‐step process that cleans the reagent nozzles and the carousel.
The first step purges each reagent line and cleans the empty carousel with methanol or
Aerofix while cycle progress is displayed on the screen. The second step, which can be
delayed indefinitely, reprimes the reagent lines with staining reagents.
NOTE: Pressing Standby/Ready performs a System Clean cycle before the instrument
goes on standby.
1 Place an empty carousel in the instrument and close the lid.

CAUTION:
Never place any carousel loaded with specimens in the instrument for a clean
cycle (including placing the instrument in standby mode). Specimens will be
damaged if they contact reagents sprayed from the nozzles when you press
Clean or Standby.

2 Press Clean.

NOTE: Pressing Stop during the Clean cycle causes the Clean Cycle Cancelled
message to be displayed. Press Clean to complete the interrupted cycle.

3 Press Reprime to reprime the lines.

e

4 Open the lid and remove the carousel when the Clean cycle is complete.

5 Spray the interior of the bowl with 70 to 100% methanol or ethanol. Wipe
the stainer bowl dry with paper towels.

NOTE: Perform the instrument storing procedure (Section 5.2), if the instrument
will remain idle for more than 1 week.

Performing Tests
We recommend performing the Pattern Test and Volume Test before using the stainer
(see Section 6).
31
SECTION 2

Reagent Level Monitoring
Reagent Level Detect monitors reagent levels and alerts you when the reagent is
running low, or when the waste container is full (when using the waste container with
level detect (AC-‐182)). You can turn reagent and waste container monitoring ON or OFF
from the Level Detect menu. The system default is ON for reagent monitoring and OFF
for waste container monitoring.

NOTE: The instrument must be installed on a flat, level surface for accurate reagent
monitoring.

CAUTION:
This system is designed to warn you when the reagent level is getting low. The
instrument will continue running through these warnings. Always monitor and replenish
(if needed) the reagent before running a stain cycle.

Enabling/Disabling Reagent Level Detect
1 Press Information from the Main menu.

2 Press System Setup.

3 Press Level Detect. The display shows:

4 Press Tray to enable, or OFF to disable a reagent line. Functions are grey
when unselected, blue when selected. Press External to enable level
detect for the waste bottle.

5 When finished, press Back to exit to System Setup menu.

32
SECTION 2

Zeroing the Reagent Level Sensors
The Level Detect function must be zeroed at initial setup, when the stainer is
moved, or if the level detect is not reporting correctly. If zeroing does not
correct the problem, recalibrate the Level Detect function (Section 7.3).

1 Press Information.


3 Press Level Detect to enter the Reagent Level Detect Setup menu.

NOTE: The stainer should be turned ON for at least 30 minutes before zeroing to
stabilize level sensors. The instrument can be used during this time.

4 Press Zero. The display shows:

33
SECTION 2

Zeroing the Reagent Level Sensors (continued)
5 Remove all reagent bottles and press Start.

NOTE: Vibrations or bumps to the instrument or lab bench can cause
inaccuracies in zeroing or calibration.

6 After zeroing, press OK. Press the Back button to exit to the System Setup
menu.

7 Return the reagent bottles to their correct positions in the tray.

NOTE: For accurate reagent level detection and calibration, dip tubes must
follow their pre-‐formed coiled shapes.

34
SECTION 3
CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS

3.1 System Setup Menu
Many software settings can be controlled from the System Setup menu, including:
• Creating, editing, and deleting stain programs
• Creating, editing, and deleting cytocentrifuge programs
• Tracking reagent information
• Managing user accounts
• Enabling tracking features for slides, maintenance, and reagents
• Managing reagent level sensing
• Changing the display language
• Viewing and exporting the system log
• Changing beeper settings
• Setting the date and time
• Restoring default settings
Accessing the System Setup Menu

1 Press System Information from the Main menu.


Stain Programs
Stain Programs allows the user to create, edit, or erase staining programs according to the
user’s specific staining requirements. Up to 12 staining programs can be programmed and
stored in memory.

Programmable staining parameters include stain intensity and fixation options.

• Stain Intensity
Selections range from 1 to 10. 1 is the lightest and 10 the darkest setting.
Settings 8, 9, and 10 are particularly appropriate for bone marrow specimens.
• Fixation
Fixation options are Off, Normal, and High.
35
SECTION 3

Creating a Stain Program
1 From System Setup, press Stain Programs.

2 Press Add.

3 Select Enter Program Name, and enter the desired name on the keypad.

4 Press Enter on the keypad. The display returns to the Stain Programming
Menu.

5 Select Intensity From the Stain Programming Menu. Select the desired
stain intensity (1-‐10).
6 Select the desired Fixation (Off, Normal, High).
7 Press Save.

Editing, Renaming, or Adjusting Stain Programs
1 From Stain Programs menu select the program to be modified.

2 Press Edit.
36
SECTION 3

Editing, Renaming, or Adjusting Stain Programs (continued)
3 Adjust the settings as needed.

4 Press Save.

Administrator and User Accounts
You can create one Administrator account and multiple (up to 50) user accounts. The
Administrator controls access to the system by adding and editing user accounts. Users
cannot edit System Settings unless permitted by the Administrator.

Creating an Administrator Account
1 From System Setup, press Users.

2 Select Lock System Setup Access.

3 Enter a password for the Administrator account (at least 4 characters) and
press Enter.

4 Re-‐enter the password and press Enter to confirm.

37
SECTION 3

Creating User Accounts
Note: This function is available only if an administrator account has been
created.

2 Enter the Administrator password.

3 Press Enter.

4 Press Users to reveal the Manage Users menu.

5 Select Enable Global Login.

6 Select Add User.

7 Enter a new user name.

8 Press Enter.
38
SECTION 3

Creating User Accounts (continued)
9 Enter a numeric passcode (at least 4 numbers) for the user account.

10 Press Enter.

11 Re-‐enter the passcode to confirm.

12 Press Enter.

Managing User Access
From the Manage Users screen, the Administrator has several options to
manage user access to the instrument.
• Enable Global Login allows users to log in to the instrument. Users will log
out manually or automatically (with user-‐selectable time options). See
User Login/Logout below.
• Enable Run Login requires the current user to enter a password to run a
Stain or Cytocentrifuge cycle. Global Login must be enabled to use this
option.
• User System Access enables complete control of the instrument, including
changing the System Setup options. This option can be controlled on an
individual user basis, if Global Login is enabled.

User Login/Logout
With System Access locked and Global Login enabled, users must log in to use
the stainer:

1 Select User ID and Select Logout time after idle from the drop-‐down
menu.

NOTE: Users can select how long the stainer can be idle before automatically
logging the user out.

39
SECTION 3

User LogIn/Logout (continued)
2 Press Login.

3 Enter the correct passcode for the selected user and press Enter.

4 Once Login is complete, the display advances to the Main screen and the
instrument is ready for programming and staining. A Logout button and
the user name appear at the top right of the Main screen.

NOTE: Users can log out manually by pressing the logout button.
40
SECTION 3

Using Reagent Information Tracking
You can enter reagent information to help track reagent usage and expiration. Reagent
information includes reference number, expiration date, lot number, date and time the
reagent was last installed.
1 From System Setup, press QC/Maintenance Tracking.

2 Select Enable Reagent Tracking by choosing reagent A, B, C, or D. This
enables reagent lot number and expiration date tracking.

3 Press Back to return to System Setup.

4 Press Reagents.

5 Press Change next to the appropriate reagent.

6 Scan the reagent bottle barcodes (Section 3.2) or manually enter the
reagent information in the correct fields.

7 Press Save for each reagent.

Modifying Level Detect Functions
The Level Detect function alerts you when the reagent is running low, or when the
waste container is almost full. You can turn reagent and waste container monitoring ON
and OFF from the System Setup screen. The system defaults to ON for reagent
monitoring and to OFF for waste container monitoring. See Section 2.2 for complete
instructions.

1 From System Setup, press Level Detect.

2 Select the reagent monitoring options to be modified.

• To disable monitoring, press OFF next to the appropriate reagents.
• To enable monitoring, press Tray next to the appropriate reagents.
• To monitor the waste container, press External.

41
SECTION 3

Changing User Language
1 From System Setup, press Language.
2 Select the software language from the list on the left.
3 Press OK.

Setting the Date and Time
1 From System Setup, press Set Date/Time.
2 Select 12 for a 12-‐hour clock or 24 for a 24-‐hour clock.
3 Use the up and down arrows to modify the time and date.
4 Press Save.

System Log
The instrument records all login, logout, stain or cytocentrifuge cycles, setting changes,
maintenance functions and specimen identification (if enabled).

Accessing Logs
1 From System Setup, press System Log.

2 Use navigation arrows to scroll through the log.

42
SECTION 3

System Log (continued)
Exporting Logs
1 From System Setup, press System Log.

2 Plug a Flash Drive into the right USB port.

3 Press Export.
NOTE: The log files are exported to the Flash Drive as a CSV file that can be used
in spreadsheet software programs.

Controlling Beeper Alerts

1 From System Setup, press Beeper.

2 Use the sliders to modify the beeper volume for Cycle Complete, Warnings,
Errors, or Key Clicks.

3 Press Beep On Startup to turn the audible startup alert ON or OFF.

43
SECTION 3

QC/Maintenance Tracking
Under system default settings, the following QC/Maintenance Tracking options are disabled:
• Stain Slide Tracking
• Cyto Slide Tracking
• Manual Entry
• Preventive Maintenance Tracking
• Reagent Tracking
Enable Stain Slide Tracking
To activate Stain Slide Tracking:
1 From System Setup, Press QC/Maintenance Tracking.

2 Press Enable Stain Slide Tracking.

3 Press Back twice to return to the main screen. Verify that the Start Button
on the main screen reads “Load Slides.”
NOTE: Selecting Enable Stain Slide Tracking changes the Start button on the
Main menu to “Load Slides.”
4 Press Load Slides. The Scan and Load Slides menu appears.

5 Enter slide information.
a. If using the barcode reader, scan the specimen slides that contain
barcodes. See Scanning Slides with the Barcode Reader (Section 3.2).
b. If entering specimen information manually, see Manually Entering
Specimen Information (Section 3.2).
6 See Section 4 for remaining steps for running a stain cycle.
44
SECTION 3

Enable Cyto Slide Tracking
Allows slide tracking in cytocentrifuge mode. See the Cytopro Rotor
Applications Manual (Aerospray Models 7xx2) (REF: RP-‐517) for complete
information.

Enable Manual Entry
If selected, allows manual entry of slide information using the keypad (limited
to 24 characters).

Enable Preventive Maintenance Tracking
To activate the tracking prompts for Preventive Maintenance Tracking, use the
following steps:
1 From System Setup, select QC/Maintenance Tracking.

2 Select Enable Preventive Maintenance Tracking.

3 Enter the information for the Daily, Weekly, and QC Slide prompts in
corresponding fields. See Using the Preventive Maintenance Log (Section
5.1).

Enable Reagent Tracking
To activate Reagent Tracking:
1 From the QC/Maintenance Tracking menu, select the reagent (A, B, C, D)
to be tracked.

45
SECTION 3

Restoring Software Defaults
1 From System Setup, select Restore Defaults.

CAUTION:
Restoring the system defaults will remove all personal settings.
• Restoring System Settings will delete all user names and passwords.
• Restoring Stain Settings will delete all stain programs and restore the
default programs.
• Restoring Cytocentrifuge Settings will delete all cytocentrifuge
programs and restore the default programs.

2 Select the settings you would like to restore to factory defaults: System
Settings, Stain Settings, or Cytocentrifuge Settings.

3 Press Restore.

4 The display returns to the Main menu.

46
SECTION 3

3.2 Recording Specimen and Reagent Information
Scanning Slides with the Barcode Reader
1 From System Setup select QC/Maintenance Tracking.

2 Select Enable Stain Slide Tracking.
NOTE: Selecting Enable Stain Slide Tracking changes the Start button on the
Main menu to Load Slides.”

3 Press Back twice to return to the Main menu.

4 Press Load Slides on the Main menu. The Scan and Load Slides menu will
appear.

5 Scan the barcode of each slide in the batch and load into the carousel
according to instructions in Section 4.1.

6 Verify that each barcode appears on the Scan and Load Slides menu.

7 When you have completed preparations to stain, (Section 4.1) press Start.
47
SECTION 3
Scanning Reagent Bottles with the Barcode Reader
1 From System Setup select QC/Maintenance Tracking.

2 Select Enable Reagent Tracking for each desired reagent (A, B, C, D).

3 Press Back to return to the System Setup menu.

4 Press Reagents to reveal the Reagent Information screen.

5 Select the desired Reagent (A, B, C, D) and press Change.

6 Scan the barcode of each enabled reagent bottle.

7 Verify that the barcode appears on the Scan and Load Slides menu.

48
SECTION 3

Scanning Reagent Bottles with the Barcode Reader (continued)
8 Press Save.

9 Repeat steps 3-‐8 for each reagent bottle that is enabled in QC
Maintenance Tracking.

NOTE: You can access Reagent Information by pressing the bottle icons on the
right side of the Main menu. This takes you directly to Reagent Information
menu, where you can scan or manually enter reagent information by pressing
Change.

Manually Entering Specimen Information
With Stain Slide Tracking and Manual Entry enabled in the QC Maintenance menu:

1 Press Load Slides on the Main menu.

2 Press Add to reveal the keypad.

3 Enter slide information (maximum of 24 characters) and press Enter.

4 To change or delete the entry, select the entry on the display and press
Edit or Remove.

5 Load slides and run stain cycle as shown in Section 4.1.

NOTE: Reagent REF number must be a valid Elitech Group REF number for
the selected reagent. Incorrect entries will generate an error message.

49
SECTION 3

Manually Entering Reagent Information
1 Press Reagents from the System Setup menu, or press the reagent status
icon on the Main menu to reveal the Reagent Information menu.

2 Select the desired reagent and press Change.

3 Select the desired field (Reagent REF, Expiration Date, Lot Number, or
Service Date/Time).

4 Enter the information on the keypad and Press Enter.

5 Press Save.
50
SECTION 3

3.3 The Help Menu
The Help menu is a comprehensive onscreen help function that provides detailed information on
the following subjects:

Help Screens
Basic Operation
• Loading the Carousel
• Programming Number of Slides
• Select Intensity Setting
• Correct Reagents and Locations
• Selecting a Staining Program
Touchscreen
Calibrating the Touchscreen
Cleaning
Clean Cycle

Using Help
1 Press Help to access the help function.

2 Select the desired topic.

3 Use the direction arrows to navigate.

4 Press Exit to return to the Main menu.
51
SECTION 4
OPERATING THE STAINER

4.1 Operating Instructions
WARNING!
Treat slides in accordance with good laboratory practices and local regulations.

Suggested Staining Protocol
• Hub Pattern Test (once per day).
• Select or verify desired stain program.
• If slide tracking is enabled, scan or enter slide information.
• Load slides into the carousel. Use blocking slides if needed.
• Place loaded carousel into the stainer and close the lid.
• Check reagent and waste levels.
• If slide tracking is not enabled, enter the number of slides on the Main menu.
• Perform a stain cycle.
• Unload the carousel.
Performing a Hub Pattern Test

Use the Hub Pattern Test to ensure the nozzles are clear of debris and spraying properly.
1 From the Maintenance menu, Press Pattern Test.

2 Hold a sheet of white paper towel near the drive hub, squarely facing the
target nozzle.

3 Press the corresponding prime button.

Check the pattern. If incorrect, clean the nozzle orifice with the nozzle brush provided in
the Nozzle Maintenance Kit. If this fails to correct the problem refer to Nozzle
Maintenance and Performance (Section 6).

Figure 7: Correct Hub Pattern Test Result

Figure 8: Incorrect Hub Pattern Test Result

52
SECTION 4

Loading the Carousel
CAUTION:
Never load chipped or cracked slides into the instrument. Slides in poor condition may
break during the staining cycle. If a slide breaks in the bowl, see Cleaning Broken Slides
(Section 5.4).

CAUTION:
Keep small ferrous metal objects away from the lab bench. These objects can be
attracted to the magnets on the bottom of the carousel and cause damage if spun free
during instrument operation.

CAUTION:
Load slides in balanced pairs. If staining an odd number of slides, use a blank slide to
balance the carousel.

NOTE: Load the carousel with similar specimens for a similar level of staining. There is no
guarantee of staining performance when dissimilar specimens are used.

1 Remove the carousel from the bowl and place it on a solid, level surface.

2 Remove the carousel lid by pressing the button and lifting the lid.

3 If Slide Tracking is enabled, press Load Slides.
• If using the barcode reader, scan each specimen slide barcode before
loading it into the carousel. Slide Tracking must be enabled from the
System Setup menu. See Enable Stain Slide Tracking (Section 3.1).

• If entering slide information manually, follow the instructions in
Section 3.2.

4 Insert the slides into the carousel with the first slide in position 1.
• Load slides in balanced pairs (directly opposite one another) to
balance the carousel. If staining an odd number of slides, use a blank
slide to balance the carousel.

• If there are empty slots in the carousel, use blocking slides to prevent
overspray (see below).
53
SECTION 4

Loading the Carousel (continued)
• Load slides with the labels toward the outside edge of the carousel.
• Always load slides with the specimen facing clockwise.
• Always place the first slide in position 1, the second in position 2, and
so on.
NOTE: A warning will sound during the staining cycle if the carousel is
unbalanced.

Figure 9: Loading the Carousel

Labeled End Away
From Center Hub

Specimen Side

Figure 10: Specimen Placement on Slide

5 Replace the carousel lid by pressing the button and lowering the lid over
the indexing posts.

6 Release the button and press the lid handle until it is firmly closed and
locked.
54
SECTION 4

Using Blocking Slides
If the carousel is not full, blank slides should be used as blocking slides. Blocking slides
prevent overspray of reagents onto the specimen slides. Overspray can cause slides to
become over-‐stained.

• Place a blocking slide in front of position 1 and 2.
Figure 11: Using Blocking Slides

B

3

2 1

4

B – Blocking Slide
1 – Slide Position 1 B
2 – Slide Position 2

Performing a Stain Cycle
1 Insert a carousel loaded with specimen slides and close the instrument lid.

2 If you have not enabled Slide Tracking, select the number of slides to be
stained. Slide selection defaults to full carousel at the end of the run, after
pressing Stop, or selecting a number greater than the full carousel default.

NOTE: To stain an odd number of specimen slides, select the next higher
number listed on the display. For example: to stain 3 slides, select 4, etc.

NOTE: If entering slide information by barcode reader or keypad, the number of
slides is programmed automatically. Adjust the total number of slides if adding
other specimen slides that have not been entered by barcode reader or keypad.

NOTE: Do not include blocking slides in the total number of slides.

55
SECTION 4

Performing a Stain Cycle (continued)
3 If you have created a stain program, and it appears on the display, proceed
to Step 4. If the desired program does not appear on the display, press
Programs. Then select the desired program and proceed to Step 4.

NOTE: No special collection, pre-‐treatment, or storage conditions are required
for specimen types. Specimens that are normally manually hematology stained
can be stained with the Hematology Stat Stainer. Adjust stain settings to
maximize staining quality for each specimen type (see Section 3.1).
4 Press Start. The display shows the progress of the program, and a signal
tone (if enabled) indicates the end of the cycle.

NOTE: Use the emergency Stop button when required, for example, if there is
abnormal vibration or noise. This will abort the staining cycle.

Unloading the Carousel

1 Remove the carousel from the bowl and place it on a solid, level surface.

2 Remove the carousel lid by pressing the button and lifting the lid.

3 Carefully remove each slide and observe the specimen using a microscope.
56
SECTION 4

Monitoring Reagent and Waste Levels
If enabled, the stainer displays the approximate reagent and waste container levels and
other information.

CAUTION:
You must monitor the reagent and waste container levels on the display (if enabled) and
by direct inspection of the bottles. The monitor will show the approximate level of each
reagent. This can be compared to the actual level in the bottles.

• Never allow a reagent to run dry. When the reagent level is near empty, replace the
reagent bottle with a new one (see below).

• Never allow the waste container level to go above the maximum safety level.
Table 11: Reagent Level Detect Display Symbols

Reagent unselected in Level Detect

Reagent Bottle Full

Reagent Bottle 2/3 Full

Reagent Bottle 1/3 Full

Reagent Bottle Empty

Measurement Error (internal level
detect problem)

Reagent has exceeded expiration
date (enabled from QC Maintenance
menu)
Waste bottle empty

Waste bottle error (such as external
bottle unplugged)

Waste bottle full

57
SECTION 4

NOTE: Access the Reagent Information menu by pressing the bottle icons on the right
side of the Main menu. Press Change to scan or manually enter reagent information.

NOTE: Do not put residual reagent from a used bottle into a new bottle. This can lead to
an accumulation of residue on the slides and may be a source of contamination.

WARNING!
Reagents used in this instrument contain moderately hazardous chemicals that
require care in handling. Always use appropriate safety measures, including gloves
and eye protection, when handling reagents.

Replacing a Reagent Bottle
1 Remove the empty reagent bottle from the tray but do not disconnect the
dip tube.

2 Open the new bottle and record the letter on the cap for future use, such
as long-‐term storage.
3 Open the Reagent Information menu by pressing the reagent bottle icon
on the right side of the Main menu.

4 Select the desired reagent and press Change.

5 If you are using reagent tracking, scan the barcode or manually enter the
reagent REF, expiration date and lot number in the Reagent Information
menu (Section 3.2).

6 Unscrew the cap and remove the dip tube from the empty bottle.

7 Insert the dip tube into the new reagent bottle and screw on the cap.

8 Place the new bottle in the tray.
Emptying the Waste Container

The Level Detect function automatically monitors the waste level and indicates when the
waste container should be emptied. It is still necessary to check waste levels visually to
ensure the waste container does not overfill.

CAUTION:
Dispose of collected waste according to local statutes and safety requirements.

1 Unscrew the cap from the full waste container.

2 Discard the waste according to local regulations.

3 Reinstall the cap on the empty waste container.
58
SECTION 5
PREVENTIVE MAINTENANCE AND SAFETY

5.1 Preventive Maintenance
The system provides a Preventive Maintenance Log for tracking the most recent maintenance
activities. See Enable Preventive Maintenance Tracking (Section 3.1) and Using the Preventive
Maintenance Log in this section.

Daily Maintenance/Quality Control (QC)
1 Check reagent levels and expiration dates.
2 Empty the waste container if necessary.
3 At the beginning of the day:

• Perform a Hub Pattern test.
• Run a QC slide if required by your laboratory.
4 If necessary, use the nozzle brush from the Maintenance Kit to clean the
nozzle orifices. Press the individual bristles into the nozzle openings.

5 At the end of the day:
• Place an empty carousel in the bowl and close the lid. Press
Standby/Ready on the front panel and wait until the end of the
automatic cleaning process.

• Spray and wipe the bowl, interior lid, and nozzles with 70 to 100%
alcohol.

• Wipe down the exterior of the instrument with 70 to 100% alcohol.

6 Ensure the maintenance procedures listed on the Maintenance Log have
been performed, and entered into the chart or log.

Weekly Maintenance
1 Wipe the carousel tray and lid using 70 to 100% alcohol.

2 Flush the waste tube with 200 to 300 mL of alcohol to prevent clogging.

3 Perform a Volume Test (Section 6.4).

4 Perform a Hub Pattern Test (Section 4.1).

5 Manually clean the nozzles if necessary.

6 Ensure the maintenance procedures listed on the Maintenance Log have
been performed, and entered into the chart or log.
59
SECTION 5

5.1 Preventive Maintenance
Monthly Maintenance
1 Disassemble and manually clean all nozzles. Refer to Nozzle Disassembly
and Cleaning (Section 6.1).

2 Disinfect any reagent bottle that is being reused (Section 5.5).

3 Ensure the maintenance procedures in the Preventive Maintenance (PM)
Log have been performed, and entered into the PM chart or log.

Using the Preventive Maintenance Log
With Preventive Maintenance Tracking enabled, the PM Log provides a convenient and
structured means of recording important maintenance and QC functions. The system
allows you to set up timely prompts that require response by the user. See Enable
Preventive Maintenance Tracking (Section 3.1).

1 From the Maintenance menu, press QC/PM to open the PM Log.

2 Press Record Maintenance.
PM Task entry options:
QC Slide Staining (Drop Down Menu)
Not Completed
Acceptable
Unacceptable
Inconclusive
Disinfect Reusable Bottles
Completed (Select/Deselect)
Drain Check
Manual Nozzle Cleaning

3 Press Save to record entries.
60
SECTION 5

5.2 Storing the Instrument
If the instrument is inactive for more than one week, you may want to perform the long-‐term
storage procedure. This will prevent nozzles from clogging when the machine is reactivated.

Preparing for Long-‐Term Storage
1 With the carousel removed, remove and clean the nozzles. Store parts in
tubes that are labeled to indicate their correct position.

2 Unscrew the cap and remove the dip tube from the bottles.

3 Place the end of the dip tube in a bottle of methanol.

4 Flush at least 250 mL of methanol through each reagent line by priming all
lines simultaneously. Leave the alcohol in the line.

CAUTION:
Leave methanol in the reagent lines during storage. Allowing reagent lines to
run dry can damage the instrument.

CAUTION:
Do not subject the instrument to freezing temperatures. Freezing of aqueous
fluids in the lines may cause damage to the instrument.

5 Flush the bowl with water.

6 Return nozzles to their original positions.
Preparing for Operation after Storage

Follow the Setup and Preparation for Operation instructions (Section 2).
61
SECTION 5

5.3 Replacing Fuses
WARNING!
To prevent the risk of fire, the main fuses should only be replaced with fuses of the same type
and rating. Recurring fuse failure indicates serious internal problems, if this occurs, contact
Elitech Group.

1 Power OFF the instrument.

2 Disconnect the power cord from the power outlet and the rear panel of
the instrument.

3 Open the fuse cover by inserting a screwdriver in the slot on the right side
of the cover and gently prying the cover open.

4 Remove the fuse holders to inspect the fuses.

Replace the fuses if necessary.

6 Push the fuse holders in.

7 Close the fuse cover.

8 Reconnect the main power cord to the rear panel of the instrument and to
the power outlet.

9 Power ON the instrument.

62
SECTION 5

5.4 Cleaning the Stainer and Carousels
WARNING!
All cleaning procedures should be performed in a well-‐ventilated room by authorized and
trained personnel wearing appropriate protection equipment.

1 Clean the outside of the instrument with 70 to 100% ethanol or methanol.

2 Clean the carousel and lid with 70 to 100% ethanol or methanol.

NOTE: Freshly prepared (< 24 hours old) 10% bleach solution can be used as
well. The 10% bleach solution helps clean the stained areas.

Cleaning Liquid Spills
Remove any liquid spilled on the instrument immediately to avoid damage to the
equipment.

WARNING!
If potentially infectious liquid is spilled on the instrument, the instrument must be
disinfected in accordance with all applicable local regulations. Refer to
Decontaminating the Stainer and Carousels (Section 5.5) for instructions.

Cleaning Broken Slides
You must take stringent precautions if a slide breaks inside the instrument during a
staining cycle, especially if the instrument has been processing dangerous pathogens.
Always use protective gloves, safety glasses, and forceps when removing broken glass
from inside the instrument.

• Glass shards embedded in the walls of the bowl can cause serious cuts and pose a
risk of infection.

• Always remove embedded shards with a scraper before attempting to remove loose
glass.

• Use a vacuum or adhesive tape to pick up loose glass inside the stainer bowl.
63
SECTION 5

5.5 Decontaminating the Stainer and Carousels
All parts of the instrument that come into contact with biological specimens, patient specimens,
positive control specimens, or hazardous material must be treated as potentially infectious.

Before the instrument is returned for service, all outer surfaces must be decontaminated. The
operating authority must complete a disinfection declaration, otherwise the instrument may be
rejected by the distributor or service center or quarantined by customs authorities.

WARNING!
Reagents used with the instrument contain moderately hazardous chemicals that require care
in handling. Always use appropriate safety measures including gloves and eye protection,
when handling reagents.

WARNING!
Authorized and trained personnel wearing appropriate protection equipment should perform
the decontamination procedure in a well-‐ventilated room. It is very important to thoroughly
decontaminate the instrument before removing it from the laboratory or before performing
any technical service. This procedure may not be effective against prions.

WARNING!
Prior to decontaminating, disconnect the instrument from the main power supply to avoid any
risk of fire or explosion.

WARNING!
The decontamination procedure and the disinfectants must comply with the local applicable
regulations.

Solutions for Decontaminating the Instrument
The outer surfaces of the instrument should be decontaminated using a
decontaminating solution such as:
• 70% ethanol or methanol
• Mild detergent
• 10% bleach solution (< 24 hours old)
• Decontamination Solution (REF: SS-‐133)
64
SECTION 5

Figure 12: Lid Latch and Locking Pin Hole Locations

1 2
1 – Lid Latch Hole
2 – Locking Pin Hole
Decontaminating the Instrument

1 Prepare a suitable container for all disposables.

2 Cover the lid latch and locking-‐pin holes with waterproof tape to protect
the interior (Figure 12).

3 Place the instrument in a biological safety hood or well-‐ventilated area.

4 Spray the inner bowl and inner lid with a decontaminating solution such as
REF: SS-‐133.

5 Repeat the spray treatment every 2 or 3 minutes for a total of 20 minutes.
Do not allow cleaning solutions to dry on the instrument surfaces.

6 Rinse the inner bowl and lid thoroughly with water.

7 Spray and wipe the exterior surfaces with decontamination solution such
as REF: SS-‐133.

CAUTION:
Do not flood the display panel with excessive moisture. Any moisture that
seeps through could damage the internal electronics.

65
SECTION 5

Decontaminating the Instrument (continued)
8 Repeat the spray treatment of exterior surfaces every 2 or 3 minutes for a
total of 20 minutes. Do not allow cleaning/decontamination solutions to
dry on the instrument surfaces.

9 Wipe surfaces thoroughly with a cloth soaked in water until you have
removed all decontamination solution.

10 Immerse or generously spray the carousel and lid with decontaminating
solution. Allow the solution to react for 20 minutes.

11 Thoroughly rinse the carousel and lid with deionized or distilled water.

Decontaminating Reagent Bottles

Any reused reagent bottles should be disinfected at each reagent lot change, or
monthly:
1 Fill the reagent bottle with a fresh (< 24 hours old) 10% bleach solution.

2 Allow the bleach solution to react in the bottle for 10 minutes.

3 Rinse the bottle thoroughly with tap water.

4 Rinse the bottle thoroughly with deionized or distilled water.
66
SECTION 5

5.6 Shipping or Disposing of the Stainer or Carousels
Shipping the Instrument or Carousels
WARNING!
You must disinfect the instrument or carousels before returning it to Elitech Group.
The operating authority must complete a Hazard-‐Free Certification form, otherwise
the distributor or service center may not accept the instrument; or customs
authorities may hold it.

CAUTION:
Shipping the instrument or carousels without decontaminating according to these
instructions is dangerous to service personnel. You will be charged additional fees for
decontamination performed by Elitech Group.

CAUTION:
Ship the instrument or carousels in containers comparable to the original packaging.
Hazard-‐Free Certification
The operating authority must print and complete the Hazard-‐Free Certification
(obtained from Elitech Group Customer Service).

Attach the certification form to the top of the instrument package before
sending the package to Elitech Group.

Disposing of the Instrument or Carousels
The instrument and carousels should be completely decontaminated and disposed of as
follows:

Under WEEE Directive 2002/96/EC, this equipment cannot be disposed of in a normal
landfill. Instead, the equipment must be disposed of either by:

1 Routing to an authorized local facility approved for handling hazardous
materials.

OR

2 Returning the equipment to Elitech Group.

67
SECTION 6
NOZZLE MAINTENANCE AND PERFORMANCE

6.1 Nozzle Disassembly and Cleaning
Nozzle maintenance requires the Nozzle Maintenance Kit and prepared Nozzle Cleaning Solution
(diluted REF: SS-‐029C).

WARNING!
Always wear protective clothing and eye protection when using Nozzle Cleaning Solution
(diluted REF: SS-‐029C). Dispose of used solution properly.

NOTE: If the compression screw cannot be easily loosened, use light penetrating oil and a 5/8-‐in.
wrench to loosen the nozzle.

Nozzle Disassembly
1 Remove the nozzle using the nozzle tool from the Nozzle Maintenance Kit.

2 Disassemble the nozzle. See Figure 5: Nozzle Diagram in Section 1.

3 If you will be storing the nozzle parts in Nozzle Cleaning solution longer
than one hour, remove the o-‐ring.

4 Place the nozzle parts (minus the o-‐ring if required) in a 50 mL conical tube
that has been clearly marked with the correct nozzle position.

5 Repeat Steps 1 through 4 for each nozzle.

Nozzle Cleaning
1 Fill each 50 mL tube with 25 mL of prepared Nozzle Cleaning Solution (REF:
SS-‐029C) and cap the tube.

2 Gently invert the tube at least ten times to ensure all parts come in contact
with the cleaning solution.

3 Place the tube in the correctly marked position in the provided tube stand.
Soak the parts as long as possible.

NOTE: Soak nozzle parts for at least 15 minutes. Parts can be soaked in Nozzle
Cleaning Solution overnight.

4 Repeat steps 1 through 3 for each nozzle.
68
SECTION 6

6.2 Nozzle Reassembly
1 Hold your thumb or a nozzle strainer over the end of the tube to keep the
nozzle parts in the tube. Discard the cleaning solution according to
applicable statutes.

Swirl Cone
Grooves (4 2 Inspect the nozzle parts. Remove any material in the swirl cone grooves by
total, 2 shown) sliding a piece of paper edgewise along each of the 4 grooves.

3 Thread the nozzle orifice cleaning wire (REF: AC-‐059) through the back of
the disassembled nozzle housing.

4 Place the nozzle parts back into the tube and rinse them with water.

5 Rinse the parts again with alcohol.

6 Apply a small amount of silicone lubricant (REF: AC-‐103) to the
compression screw threads.

Apply Silicone 7 Reassemble the nozzle by placing the compression screw on the nozzle
Lubricant Here disassembly tool, then inserting the swirl cone into the compression screw.

Nozzle
NOTE: Hold all the parts in a vertical position during reassembly.
Disassembly

Tool
8 Reinstall the nozzle housing over the swirl cone and compression screw.

9 Replace the o-‐ring if it was removed earlier.

10 Return the assembled nozzle to its original position in the instrument.
D C B A


12 Perform a Hub Pattern Test (Section 4.1).

13 Perform a Volume Test (Section 6.4).

NOTE: You must perform the Hub Pattern Test and Volume Test before
operating the instrument. If the results are incorrect, manually prime the
instrument.
69
SECTION 6

6.3 Manual Priming
1 Remove the carousel from the bowl.

2 Remove the nozzle connected to the line to be manually primed.

3 Insert the priming tool nozzle adapter (included in the Nozzle Maintenance
Kit) into the nozzle holder and turn it clockwise to install the adapter into
the holder.

4 Withdraw the priming tool plunger halfway to create a vacuum. Hold the
plunger in that position.

5 Press Maintenance from the Main menu.

6 Press Volume Test.

7 Press the desired prime button to start the reagent pump.

8 Run the reagent into the tube until the fluid is free of bubbles, then press
Stop.

WARNING!
Do not pull the plunger completely out of the priming tool. Pulling the
plunger out of the tool may result in splashing or spraying of reagents. Do not
push the plunger in while it is connected to the nozzle holder.

9 Turn the nozzle adapter counterclockwise to remove it from the nozzle
holder.

10 Discard the collected fluid into the stainer bowl.

11 Reinstall the nozzle.

12 Perform a Hub Pattern Test.

13 Perform a Volume Test.

70
SECTION 6

6.4 Performing the Volume Test
The Volume Test requires the Nozzle Maintenance Kit.

NOTE: The Volume Test must be performed weekly.

1 From the Maintenance menu, select Volume Test.

2 Hold a Volume Test tube (small tube) to cover the selected nozzle.

3 Press the corresponding reagent prime button to collect the reagent.

NOTE: With QC/Maintenance tracking enabled, enter the measured volume on
the keypad and press ENTER. With QC/Maintenance Tracking disabled, the
menu returns to the Maintenance menu.

4 Remove and cap the tube.

5 Record the nozzle position on the tube and place the tube in the
appropriate position in the tube stand.


7 Compare collected nozzle volumes with the following table.

Table 12: Volume Test Tolerances

Nozzle/Reagent Line Minimum Maximum
A, B, C 9.0 mL 11.0 mL
D 8.5 mL 10.5 mL
NOTE: The stainer normally functions correctly if nozzle volumes are slightly
higher or lower than the specified range. It is important that the B and C nozzle
volumes are similar, (typically within 1 mL). Spray volumes < 7.5 mL or > 13.0
mL indicate serious problems with the nozzles or reagent delivery lines.
• If the volume is within the tolerance range, go to Step 9.
• If the volume is outside the tolerance range:
a. Clear the nozzle orifice with the nozzle brush found in the
maintenance kit.

b. If necessary, remove the nozzle and perform the Nozzle
Cleaning procedure (Section 6.1).

c. If the problem persists, replace the nozzle.

NOTE: If the problem persists after you have replaced the nozzle, contact Elitech
Group.
71
SECTION 6

6.4 Performing the Volume Test
8 Prepare the Maintenance Kit for future use:
• Empty the contents of the tubes into the stainer bowl.
• Rinse the tubes with water.
• Put the tubes back into their original place in the Maintenance Kit or
tube stand.

9 Press Back twice to return to the Main menu.
72
SECTION 6

6.5 Performing the Slide Pattern Test
This test can differentiate poor staining results from sample preparation problems, or nozzle
obstructions. Perform the Slide Pattern test when a Hub Pattern test produces a normal result,
but staining is still inadequate.

1 Place a 1 x 3 inch (2.5 x 7.6 cm) piece of paper (REF: RP-‐500) in positions 1
and 2 of the carousel, with a blocking slide in front of positions 1 and 2.

2 Load the carousel into the stainer and close the lid.

3 From the Main menu, press Maintenance.

4 Press Pattern Test.

5 Press the corresponding prime button for the reagent line to be tested.

6 Remove the paper slides.

7 Repeat Steps 1 through 6 for each reagent line.

8 Examine the paper slides for each reagent. The pattern on the slide should
be uniform, without any continuous lines or streaks.

Figure 13: Correct Slide Pattern Test Result

Figure 14: Incorrect Slide Pattern Test Result

9 If the result is incorrect, clear the nozzle blockage using the nozzle brush,
or disassemble and clean the nozzle (Section 6.1).
73
SECTION 7
SOLVING PROBLEMS

7.1 Troubleshooting
The following table is to help identify and solve routine problems with the stainer. More difficult
problems may require technical service. Contact your Elitech Group representative for
assistance.

WARNING!
Due to the electrical shock hazard, do not open this instrument or attempt internal repairs.
Refer servicing to qualified service personnel. Contact your dealer or Elitech Group Service.

Table 13: General Troubleshooting and Diagnosis

Problem Solution
There is no power to the stainer when the power switch Check the facility outlet and the power cord connection.
is turned ON. Check the fuses. Refer to the Replacing Fuses procedure.
CAUTION:
Fuse failure may indicate a serious internal problem.

Strange information shows on the display, and/or erratic Switch the power OFF, wait 10 to 20 seconds, then
stainer operation. switch power ON again. If problem recurs, install a
computer-‐type surge suppressor to protect the
instrument from power line transients. If possible,
connect the stainer to a power circuit that is not shared
by centrifuges, refrigerators, air conditioners, or other
motorized equipment.

If the above steps do not solve the problem, consult the
Aerospray Service Manual, or contact your dealer or
Elitech Group for assistance.

A reagent line will not prime when power is ON and you Follow the procedures in Section 6.3 for priming reagent
press the prime button. pumps.

A reagent line will not prime, even with the priming tool Press the priming button and listen carefully for the
(Section 6.3). sound of the pump. If you can hear the pump, try the
priming tool again. If the problem is not solved or if you
cannot hear the pump there may be an internal problem.
Contact your dealer or Elitech Group for assistance.

Stainer bowl fills with reagent after use. A small puddle of stain around the drain inlet or the
bottom of the bowl is normal. If the bowl is filling with a
large quantity of stain, check the external drain tube for
blockage. Make sure the drain tube is properly
connected and running continuously down toward the
lab drain or vented waste container, with no loops, rises,
or obstructions. Make sure the end of the tube is not
submerged. This can prevent proper drainage. The
internal drain may need to be cleaned or replaced. See
the Aerospray Service Manual, or contact your dealer or
Elitech Group Service.

74
SECTION 7
SOLVING PROBLEMS

7.1 Troubleshooting
Table 13: General Troubleshooting and Diagnosis (continued)
Problem Solution
Stain is leaking onto the counter. Check all external reagent lines for visible signs of cracks
or loose fittings.

Make sure the drain outlet is not blocked.

Make sure the drain tube is securely attached to the
drain port and that the tubing is not cracked or
deformed.

Reagent leaks may indicate an internal problem (see
Section 7.3). See the Aerospray Service Manual, or
contact your dealer or Elitech Group for further
assistance.

Error messages on the screen. If the display shows Lid Not Shut: Verify that the lid is
fully closed and latched. If the Lid Not Shut indication
remains, contact Elitech Group for assistance.

If the display shows Wrong Rotor after pressing Start:
Make sure the slide carousel is properly loaded on the
drive hub. In staining mode, the instrument detects
whether the staining carousel is present before
proceeding. In cytocentrifuge mode, the instrument will
stop if it senses the staining carousel. After verifying the
carousel is correctly loaded, press Start. If the display still
shows Wrong Rotor, there may be an internal problem.
Check for missing carousel magnets.
The microprocessor monitors carousel rotation during a
staining cycle. The display shows an error message if the
rotation is not within the specified range.

If the display shows Motor Drive Error: Check the stainer
bowl for interference: Turn the hub or carousel by hand;
it should turn freely.
Drive motor or electronic component malfunctions
require servicing of internal components. Contact your
dealer or Elitech Group for assistance.

If the display shows Rotor Imbalance, make certain the
Cytopro rotor is balanced, or the staining carousel is
seated correctly on the hub.
See Electronic Failure later in this table.

75
SECTION 7
SOLVING PROBLEMS

7.1 Troubleshooting
Problem Solution
The stainer fails to spray reagent during a staining cycle To allow programmed staining of partial loads, the
and/or continues to run after the cycle should be stainer monitors the position of the carousel as it rotates
complete. in the bowl. In normal operation, stain is sprayed only in
the correct position. This causes the actual cycle time to
vary, depending on the position of the carousel at the
beginning of the cycle. However, if the cycle continues
for an abnormally long period, or if the bar graph and
percentage complete icon do not change after 1 minute,
it may indicate an electronic problem or an internal
problem. To determine this, press Stop.

If the cycle stops: this indicates a problem with the
carousel position sensor. Consult the Aerospray Service
Manual, or contact your dealer or Elitech Group for
assistance.

If the cycle continues: this indicates an electronic
problem (see below).
Abnormal staining on entire surface of all slides. Check the reagent level on the display and/or in the
reagent bottles.

Make sure the external reagent dip tubes are securely
attached to each bottle (Section 2.1).

Open the lid and verify that each reagent pump is
primed, by pressing the corresponding prime button. The
nozzle should immediately spray a fine mist of reagent.
There should be no sputtering or hissing sounds
indicating air in the reagent lines.

Watch the external tubes for air bubbles. Air bubbles
indicate inadequate priming or possibly an air or reagent
leak in the system. Air in any reagent line will cause poor
staining.
Check nozzle performance using the Slide Pattern
(Section 6.5) and Volume Tests (Section 6.4). If necessary,
clean nozzle(s) using the procedures in Section 6.1.

Verify that each reagent dip tube vent hole is clear.

76
SECTION 7
SOLVING PROBLEMS

7.1 Troubleshooting
Problem Solution
Abnormal staining on entire surface of all slides When staining a full carousel (7 or more slides) make
(continued). certain you have not programmed the stainer for fewer
slides.

If staining a partial load, load the slides in the correct
positions as indicated by the markings on the carousel
(see Section 4.1).

Abnormal staining on entire surface of some slides, while Make certain that all position magnets are still attached
other slides from the same carousel appear normal. to the bottom of the carousel. Make certain you have not
programmed the stainer for fewer slides than you have
loaded.

If you program the stainer for a partial load, load the
slides in the correct positions as indicated by the
markings on the carousel (see Section 4.1).

Verify that each reagent pump is primed by opening the
lid and pressing the corresponding prime button. The
nozzle should immediately spray a fine mist of reagent.
There should be no sputtering or hissing sounds to
indicate the presence of air in the reagent lines (see
Section 7.3).

Streaks or bands of discoloration on one or more slides. Check the level of the Rinse (Reagent A) bottle. Check
Rinse (Reagent A) spray volume according to Section 6.4.

Check nozzle spray pattern according to the procedures
in Sections 4.1 and 6.5. This type of discoloration is
usually caused by debris or reagent precipitate clogging
the spray nozzle orifice.

Clean any nozzle that exhibits a poor spray pattern.

Cells are washing off slides. Check methanol or Aerofix (Reagent D) level in bottle.

Check Reagent D nozzle spray volume and slide pattern
(see Section 6.4 and Section 6.5).

77
SECTION 7
SOLVING PROBLEMS

7.1 Troubleshooting
Problem Solution
Cells are washing off slides (continued). Check the methanol or Aerofix (Reagent D) hub pattern
and spray volume (see Sections 4.1 and 6.5).
Make certain the blood smears are completely dry and
not too thick (see Section 7.2).
Increase the fixation setting to High.

Call Elitech Group for information on slide quality.

Refractile artifacts are observed in erythrocytes. Use Aerofix (SS-‐048 or SS-‐148) fixative. Refer to Section
7.2.

High levels of precipitates observed. Discard leftover thiazin stain (Reagent B) in the used
bottle when changing to a fresh bottle of stain. While
transferring remaining stain saves stain, it eventually
concentrates the precipitates to the point they appear on
the slide.

Staining intensity is too weak. Granulation is weak. Increase the intensity setting.
Refractile artifacts are observed in erythrocytes when Mix 1 part Aerofix (SS-‐048) with 3 parts Basofix predip
using Basofix predip (SS-‐049P). (SS-‐049P), or add 5 mL Aerofix concentrate (SS-‐148) to
500 mL Basofix predip (SS-‐049P).

Increased false-‐positives for Howell-‐Jolly bodies or Disinfect reagent lines using instructions in Section 7.2.
amorphous precipitates are observed.
Discard leftover thiazin stain (Reagent B) in the used
bottle when changing to a fresh bottle of stain. While
transferring remaining stain saves stain, it eventually
concentrates the precipitates to the point where they
can be seen on the slide.

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SECTION 7
SOLVING PROBLEMS

7.1 Troubleshooting
Problem Solution
Electronic Failure • An electronic failure would appear as an obvious
malfunction such as a scrambled or totally inoperative
display panel.
•
• Transient voltages coming through the power lines may
cause the stainer to “lose its place.”
1 If this occurs, switch the main power OFF for 10-‐20
seconds and then back ON to reset the instrument.

2 If the problem recurs, install a computer-‐type surge
protector to isolate the instrument.

3 If possible, connect the stainer to a power circuit not
shared by centrifuges, refrigerators, air conditioners,
or other motorized equipment.
For more obscure electronic problems, monitor the
stainer through a complete staining cycle to determine if
the operating sequence is correct. Do this by running the
stainer while watching the display and listening to the
pumps.

Ensure that each event occurs according to the operating
sequence, shown in Table 3 in Section 1.

If the problem recurs, contact your dealer or Elitech
Group for assistance.

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SECTION 7
SOLVING PROBLEMS

7.2 Abnormal Staining Results
Smear Separation or Tearing
Losing blood cells from the slide surface during a staining cycle can happen for several
reasons:

Improper Alcohol Fixation
If the alcohol-‐fix phase of the cycle fails, there will be cell loss when stain is
applied. Verify that the alcohol nozzle is operating properly with normal spray
pattern and spray volume (Sections 6.4, 6.5). To identify the problem as a
fixation failure, fix some specimen slides in methanol prior to loading into the
Wet Smears
carousel. Select a fixation setting that increases fixation (7, 8, or 9).

Wet Smears
Loading wet blood smears into the carousel may contribute to smear tearing.
Allow smears to dry for several minutes before beginning a stain cycle.

Thick Smears
Smears that are too thick can cause smear separation. If the smear has a thick
“wedge” cross section, cells can be torn away from the slide surface in the
thicker regions, while the thinner “monolayered” regions remain usable. Severe
tearing that starts in the thick region may also extend into the monolayer.

Dirty Slides
Dirty slides are a major source of cell loss during staining. We strongly
recommend that you use new, premium quality slides.

Even with premium quality slides, random loss may occur due to inadequate
slide surface quality. When the stainer is functioning properly, slide quality
accounts for most, if not all, cell loss problems.

Red Cell Artifacts
Refractile bodies or inclusions (seen as dark ringed objects on red cells) are
thought to be caused by the presence of water during fixation. Often called
“water spotting,” this effect is common in Romanowsky staining. Methanol
used for fixation must be anhydrous (0.5% or less water).
Methanol that is exposed to the atmosphere will absorb a considerable amount
of water, particularly when the relative humidity is high. The first line of
defense to avoid water spotting is to make certain the methanol used for fixing
smears is free of water. If in doubt, replace it with reagent known to be
anhydrous.

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SECTION 7
SOLVING PROBLEMS

Red Cell Artifacts (continued)
When the relative humidity is above 60 percent, water spotting may be seen
even when using anhydrous methanol for fixation. This occurs because of
moisture accumulation and condensation inside the carousel chamber. Relative
humidity within the chamber will tend to build up with repeated use, leading to
further problems. If water spotting occurs:

Use Aerofix®
Water spotting can be eliminated by using Aerofix. Aerofix is available as an
additive (REF: SS-‐148, 135 mL bottle, enough for nine 500 mL bottles of
methanol) or premixed with methanol (REF: SS-‐048, 500 mL bottle).

• Pre-‐fix Slides
Water spotting can usually be eliminated by pre-‐fixing slides in anhydrous
methanol before loading into the carousel. Allow the slides to dry for 2 to 3
minutes before staining.

• Wipe Carousel Chamber Dry
Between cycles, leave the stainer lid open to dry out the bowl. If necessary,
wipe the interior of the carousel chamber dry to reduce the relative
humidity. Or, irrigate the chamber with methanol from a wash bottle.

False-‐Positive Howell-‐Jolly Bodies or Amorphous Precipitates
Artifactual Howell-‐Jolly bodies can be caused by precipitate build up from
transferring the residual thiazin stain (Reagent B) to the fresh bottle when
reloading reagents. Such artifacts may also indicate microbial contamination of
the reagent lines and pumps.

If such artifacts are encountered, and if you suspect microbial contamination,
use the following solution to disinfect the reagent lines and pumps.

Replace precipitated stain with fresh stains after flushing the B line with the
disinfecting solution.

Disinfectant Solution:
10% freshly prepared dilution of household bleach (5.25% Sodium
Hypochlorite).

Dilution:
100 mL household bleach
+ 900 mL deionized water

= 1000 mL
81
SECTION 7
SOLVING PROBLEMS


Procedure:
NOTE: Do not remove the nozzles.

1 Pump 150 mL of prepared solution through each affected reagent line (A, B
and C). Wait 20 minutes.

2 Pump another 100 mL of disinfectant solution through each affected
reagent line and let sit for 20 minutes.

3 Flush each affected reagent line with 150 mL of deionized water.

4 Reprime the stainer with fresh reagents (see Section 2.2) and clean nozzles
according to instructions in Section 6.1 and 6.2.

5 Perform a Volume Test according to instructions in Section 6.4 and record
on PM Chart.

CAUTION!
To avoid damage to the instrument, do not leave disinfectant solution in the
reagent lines longer than recommended. This procedure should only be
performed on an “as needed” basis.

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SOLVING PROBLEMS

7.3 Instrument Malfunction
Air or Reagent Leaks
Repriming the instrument is usually unnecessary unless a reagent bottle runs completely
dry.

An air leak is usually to blame if a smooth and continuous liquid spray fails to come from
the nozzles. Carefully inspect all components in the external reagent delivery lines. Look
for loose connections, cracks, or breaks that might allow air to be drawn in when the
pump operates. Replace any defective part or assembly.

An internal leak may cause fluid to leak from the line when the pump is not running. If
an abnormal liquid spray still occurs after all the external reagent delivery line
components have been verified, the instrument may require service. Contact your
dealer or Elitech Group for assistance.
Reagent Delivery L ines
A reagent line leak between the pump outlet and the nozzle will cause fluid to leak into
the interior of the stainer housing and ultimately onto the counter. If you observe this,
the instrument will require service. Contact your dealer or Elitech Group for assistance.

WARNING!
A break or malfunction in the reagent delivery system can potentially release up to
500 mL of highly flammable anhydrous alcohol in and around the instrument. If this
occurs, carefully shut off the power to the instrument and consult the SDS for
information in handling alcohol spills. Do not use the instrument again until any leaks
are repaired.

WARNING!
Electrical shock hazard⎯ do not open this instrument or attempt internal repairs.
Refer servicing to qualified service personnel. Contact your dealer or Elitech Group
Service.
83
SECTION 7
SOLVING PROBLEMS

Reagent Level Detect System Errors
Reagent A-‐D Not Calibrated
During the second part of calibration, if no bottles are detected, the display shows an
error message.

Calibrate again, making sure that the reagent bottles are inserted in those tray positions
that have been enabled in the level detection system.

Level Detection (LD) Unstable
If movement was detected on bottles while calibrating/zeroing, the display shows an
error message.

NOTE:
While zeroing or calibrating, do not bump the instrument or lab bench. Ensure that no
nearby equipment vibrations can be transmitted to the stainer.

Calibrating the Reagent Level Detect System
If the Reagent Level Detect System is reporting incorrectly and zeroing (Section 2.2)
does not correct the problem, calibrate the system as follows:

1 Press System Information from the Main menu.


3 Press Level Detect.

4 Press Calibrate. Follow the display prompts.

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SOLVING PROBLEMS

Calibrating the Reagent Level Detect System (continued)
5 Remove all reagent bottles and press Start.

NOTE: Any vibrations or bumps to the instrument or lab bench can cause
inaccuracies in zeroing or calibration.

NOTE: Calibration requires full, unopened (caps and seals in place) 500 mL
bottles of reagent, placed in the correct tray positions (due to different densities
of each reagent type).

6 Place the correct reagent bottles in all enabled positions, and press Start.

NOTE: The calibration function ignores any disabled reagent line.

7 Press OK. Press Back twice to return to the Main menu.

8 Return the reagent bottles to the tray as indicated in Section 2.1 to
prepare for staining.

NOTE: For accurate reagent level detection and calibration, dip tubes must
follow their pre-‐formed coiled shapes.
85
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SOLVING PROBLEMS

7.4 Calibrating the Touchscreen
1 Press and hold Standby/Ready for 5 seconds. A calibration screen with a
target appears.

2 Press the center of the target with a finger, stylus, or similar tool. Another
target will appear in a different location.

3 Continue to press the center of the targets until you have pressed all the
targets (five total). After the fifth target is pressed, the instrument will
save the touch screen calibration and return to the Main menu.

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SOLVING PROBLEMS

7.5 Service Information
Elitech Group’s Service Department will help you resolve any questions about the operation or
performance of your Aerospray Stainer/Cytocentrifuge.

Customers in the United States should contact us by telephone. Outside the U.S., our authorized
dealers offer full local service and support.

ELITechGroup Inc.
370 West 1700 South
Logan, Utah 84321-‐8212 USA

Telephone:
800 453 2725 (United States & Canada)
(+1) 435 752 6011 (International calls)

Fax:
(+1) 435 752 4127

Email:
[email protected] (Service)
[email protected] (Sales)

Web Page:
www.elitechgroup.com

European Authorized Representative:
Medical Technology Promedt Consulting
Altenhofstr. 80
D-‐66386 St. Ingbert
Germany

Telephone: +49(0)68 94-‐58 10 20
Fax: +49(0)68 94-‐58 10 21
Email: info@mt-‐procons.com
87
SECTION 8
CYTOPRO® CYTOCENTRIFUGE

8.1 Cytopro Cytocentrifuge Information
Functional Description
The Cytopro Cytocentrifuge rotor allows rapid sedimentation of specimen cells onto
microscope slides for staining or other purposes. Up to eight disposable/reusable
sample chamber assemblies with absorbent pads and glass microscope slides can be
loaded into the Cytocentrifuge rotor.
Cytocentrifuge and staining functions are independent of one another.
The Cytopro rotor reduces cell loss during collection and prevents accidental damage
to the collected specimen. The rotor is sealed to control aerosol release during
cytocentrifugation. See the Cytopro Rotor Applications Manual (Aerospray Models
7xx2) (RP-‐517) for complete information.

Key Features
Adding the Cytopro Cytocentrifuge rotor transforms the stainer into a standard
cytocentrifuge with:
• Single, Dual, and Cytopro Magnum chambers
• Reusable or disposable chambers (single and dual)
• Eight slides and chambers
• User-‐programmable memory locations for settings (speed, acceleration rate, and
time)
• Easy switching between staining and cytocentrifuge modes
• Autoclavable rotor
NOTE: Pressing Cyto brings up the Cytocentrifuge mode. Pressing Back returns to stain
mode.

WARNING!
The Cytopro rotor lid, rotor gaskets and related components are intended to be part of
biosafety system as specified in international and national biosafety guidelines. They
cannot be relied on as the only means of safeguarding workers and the environment
when handling pathogenic microorganisms.

Intended Use
The Cytopro Cytocentrifuge rotor is an in vitro diagnostic medical device for
professional use only. It is an accessory for fixing biological cell suspensions on glass
microscope slides for cytological examination.

The Cytocentrifuge rotor can be used with the following cell suspensions:
• Bronchoalveolar liquid (BAL)
• Cerebrospinal fluid (CSF)
• Urine
• Synovial fluid
• Others
88
APPENDIX A
Critical Reagent Components
The following information identifies the critical chemicals of each reagent used in this instrument.
Reagent(s) Critical Components
SS-‐035A, SS-‐035A-‐EU, SS-‐035AG, or SS-‐135/149A (as <1% Potassium Phosphate
diluted) Hematology Reagent A Rinse contains: <1% Sodium Phosphate
<1% Eosin
<1% Benzyl Alcohol

SS-‐035/049B, SS-‐035/049B-‐EU, SS-‐035/049BG, or SS-‐ <1% Potassium Phosphate
135/149B (as diluted) Hematology Reagent B Thiazin <1% Sodium Phosphate
Stain contains: <1% Azure B
<1% Methylene Blue
<1% Benzyl Alcohol
<0.1% Triton X-‐100

SS-‐035C, SS-‐035C-‐EU, SS-‐035CG, or SS-‐135/149C (as <1% Potassium Phosphate
diluted) Hematology Reagent C Eosin Stain contains: <1% Sodium Phosphate
<1% Eosin
<0.2% Formaldehyde
<0.1% Triton X-‐100

SS-‐048, SS-‐048-‐EU, or SS-‐148 (as diluted) Aerofix >95% Methyl Alcohol
Fixative High Humidity contains: <4% Ethylene Glycol
<2% Polyvinylpyrrolidone (PVP)
<1% Azure B

SS-‐049A, SS-‐049A-‐EU, or SS-‐049AG or SS-‐135/149A (as <3% Ethyl Alcohol
diluted) Basofix Reagent A contains: <3% Methyl Alcohol
<1% Potassium Phosphate
<1% Sodium Phosphate
<1% Eosin
< 0.2% Formalin

SS-‐049C, SS-‐049C-‐EU, or SS-‐049CG or SS-‐135/149C (as <10% Diethylene Glycol
diluted) Reagent C Basofix Eosin Stain contains: <5% Ethyl Alcohol
<5% Methyl Alcohol
<1% Potassium Phosphate
<1% Sodium Phosphate
<1% Eosin Y

SS-‐049P, SS-‐049P-‐EU, or SS-‐149P (as diluted) Basofix <99% Methyl Alcohol
Pre-‐dip Fixative contains: <1% Polyvinylpyrrolidone
<1% Azure B

SS-‐135/149A, Reagent A Rinse Reagent Concentrate <1% Potassium Phosphate
contains: <1% Sodium Phosphate
<0.2% Formalin
<1% Eosin

SS-‐135/149B, Reagent B Thiazin Stain Concentrate <10% Potassium Phosphate
<2% Azure B
<1% Methylene Blue

89
APPENDIX A
Critical Reagent Components
Reagent(s) Critical Components
SS-‐135/149C, Reagent C Eosin Stain Concentrate <10% Potassium Phosphate
<0.2% Formaldehyde
<3% Eosin

SS-‐135S Surfactant contains: <70% Germall II (Diazolidinyl Urea)
<5% Triton X-‐100

SS-‐148, Aerofix Fixative Concentrate High Humidity 55-‐70% Ethylene Glycol
contains: 30-‐45% Polyvinylpyrrolidone (PVP)
<1% Azure B

SS-‐029, SS-‐029C and SS-‐029CG (as diluted) Nozzle 40-‐50% Methyl Alcohol
Cleaning Solution contains: 1-‐5% Oxalic Acid

SS-‐029C, SS-‐029CG Nozzle Cleaning Solution 95-‐99% Deionized Water
Concentrate contains: 1-‐5% Oxalic Acid

SS-‐133 Decontamination Solution when diluted as <2% Germicidal Detergent
directed contains: >98% Deionized Water

SS-‐MeOH Anhydrous Methanol contains: ≥99.5% Methyl Alcohol, Anhydrous

SS-‐133 Decontamination Solution Concentrate <30% Germicidal Detergent
contains: >70% Deionized Water

90
APPENDIX B
Risk and Safety Phrases
Reagents SS-‐035A, SS-‐035A-‐EU, and SS-‐035AG Reagent – A Rinse, and SS-‐135/149A – Rinse Reagent
concentrate after dilution as directed, are not associated with any Risk and Safety Phrases. They are not
associated with any EU Symbols of Danger.

R Phrases: None
S Phrases: None

Reagents SS-‐035/049B, SS-‐035/049B-‐EU, and SS-‐035/049BG – Reagent B Thiazin Stain, and SS-‐135/149A –
Rinse Reagent concentrate after dilution as directed, are not associated with any Risk and Safety Phrases.
They are not associated with any EU Symbols of Danger.

R Phrases: None
S Phrases: None
Reagents SS-‐035C, SS-‐035C-‐EU, and SS-‐035CG – Reagent C Eosin Stain, and SS-‐135/149A – Rinse Reagent
R Phrases: None
S Phrases: None

SS-‐MeOH Aerospray Reagent-‐Grade Methanol is associated with the following Risk and Safety Phrases.
European Symbols of Danger are: F and T.

R 11: Highly Flammable
R 23/24/25: Toxic by inhalation, in contact with skin and if swallowed
R 39/23/24/25: Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and
if swallowed
S 7: Keep container tightly closed
S 16: Keep away from sources of ignition – No smoking
S 36/37: Wear suitable protective clothing and gloves
S 45: In case of accident or if you feel unwell, seek medical advice immediately (show label where
possible)

Reagents SS-‐048 or SS-‐048-‐EU – Aerofix Fixative, 500 mL, and SS-‐148 – Aerofix Additive for methanol
reagent after dilution as directed, are associated with the following Risk and Safety Phrases. The associated
EU Symbols of Danger are F and T.

if swallowed
possible)

Reagents SS-‐049A, SS-‐049AG, SS-‐049A-‐EU– Basofix Rinse Reagent, and SS-‐135/149A – Rinse Reagent

R Phrases: None
S Phrases: None

91
APPENDIX B
Reagents SS-‐049C and SS-‐049C-‐EU – Basofix Eosin Stain, SS-‐049CG or SS-‐135/149C – Eosin Stain concentrate
after dilution as directed, are associated with the following Risk and Safety Phrases. The associated EU
Symbol of Danger is Xn.

R 20/21/22: Harmful by inhalation, in contact with skin and if swallowed
R 68/20/21/22: Harmful: possible risk of irreversible effects through inhalation, in contact with skin and if
swallowed
S 45: In case of accident or if you feel unwell seek medical advice immediately (show the label
where possible)

Reagents SS-‐049P and SS-‐049P-‐EU – Basofix Pre-‐dip Fixative and SS-‐149P – Basofix Pre-‐dip Fixative
concentrated when diluted as directed are associated with the following Risk and Safety Phrases. The
associated EU Symbols of Danger are F and T.

if swallowed
possible)

Reagents SS-‐135/149A – Rinse Reagent Concentrate is not associated with any Risk and Safety Phrases. It is
not associated with any EU Symbols of Danger.

R Phrases: None
S Phrases: None

Reagents SS-‐135/149B – Thiazin Stain Reagent Concentrate is not associated with any Risk and Safety
Phrases. It is not associated with any EU Symbols of Danger.

R Phrases: None
S Phrases: None

Reagents SS-‐135/149C – Eosin Stain Reagent Concentrate is not associated with any Risk and Safety
Phrases. It is not associated with any EU Symbols of Danger.

R Phrases: None
S Phrases: None

Reagent SS-‐135S – Hematology Surfactant is associated with the following Risk and Safety Phrases. The
associated EU Symbol of Danger is Xi.

R 43: May cause sensitization by skin contact
R 52/53: Harmful to aquatic organisms, may cause long-‐term adverse effects in the aquatic
environment
S 61: Avoid release to the environment. Refer to special instructions/safety data sheet

92
APPENDIX B

Reagents SS-‐148 – Aerofix Additive for Methanol is associated with the following Risk and Safety Phrases. The
associated EU Symbol of Danger is Xn.

R 22: Harmful if swallowed
S phrases: None

SS-‐029 Aerospray Nozzle Cleaning Solution and SS-‐029C when diluted as instructed are associated with the
following Risk and Safety Phrases. European Symbols of Danger are: F and T.

R 23/24/25 Toxic by inhalation, in contact with skin and if swallowed
if swallowed
possible)

SS-‐029C and SS-‐029C-‐EU Aerospray Nozzle Cleaning Solution is associated with the following Risk and Safety
Phrases. European Symbol of Danger is: None.

R: None
S: None

SS-‐133 Decontamination Solution Concentrate is associated with the following Risk and Safety Phrases.
European Symbols of Danger is C

R 34 Causes burns
R 22 Harmful if swallowed
R 67 Vapors may cause drowsiness and dizziness
S 24/25 Avoid contact with skin and eyes
S 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
S 36/37/39 Wear suitable protective clothing, gloves and eye/face protection
S 45 In case of accident or if you feel unwell seek medical advice immediately (show the label
where possible)

SS-‐103 O-‐ring/Nozzle Thread Grease is associated with the following Risk and Safety Phrases. European
Symbols of Danger are: None.

R: None
S: None

SS-‐149P Basofix Pre-‐dip Fixative Concentrate when diluted as instructed is associated with the following
Risk and Safety Phrases. European Symbols of Danger are: None.

R: None
S: None

93
APPENDIX C
Accessories and Supplies
Only replacement parts supplied by Elitech Group should be used in this instrument. Use of non-‐approved
parts may affect the performance and safety features of this product.

ACCESSORIES REFERENCE NUMBER
Slide Carousel (12-‐Slide Capacity) ........................................................... AC-‐028
Nozzle Tool .............................................................................................. AC-‐034
Nozzle Hex Wrench ................................................................................. AC-‐035
5 L Space-‐Saver Container w/cap (For Concentrate Reagents) ............... AC-‐038
Drain Tube, 1.8 meter (6 foot) Length ..................................................... AC-‐041
500 mL bottle with cap (pack of 5 bottles) .............................................. AC-‐043-‐05
Slide Carousel (30-‐Slide Capacity) ........................................................... AC-‐057
Nozzle Orifice Cleaning Wire ................................................................... AC-‐059
Reagent Pump Priming Tool .................................................................... AC-‐069
Aerospray/Cytopro Safety Shield ............................................................ AC-‐110
Cytopro Cytocentrifuge Rotor ................................................................. AC-‐160
10 L Waste Container (without level detect) .......................................... AC-‐170
1D Barcode Scanner ................................................................................ AC-‐181
10 L Waste Container (with level detect) ................................................ AC-‐182
Nozzle Maintenance Kit ........................................................................... AC-‐184
2D Barcode Scanner ................................................................................ AC-‐185

SUPPLIES
Nozzle Cleaning Solution, 355 mL ........................................................... SS-‐029
Nozzle Cleaning Solution Concentrate, 250 mL (Dilutes to 500 mL) ....... SS-‐029C or SS-‐029C-‐EU
Nozzle Cleaning Solution Concentrate, 1.89 L (Dilutes to 3.785 L) ......... SS-‐029CG
Reagent A (Rinse), 500 mL ....................................................................... SS-‐035A or SS-‐035A-‐EU
Reagent B (Thiazin Stain), 500 mL ........................................................... SS-‐035/049B or SS-‐035/049B-‐EU
Reagent C (Eosin Stain), 500 mL .............................................................. SS-‐035C or SS-‐035C-‐EU
Reagent C (Eosin Stain), 3.785 L (1 gal) ................................................... SS-‐035CG
Reagent D (Methanol), 500 mL ............................................................... SS-‐MEOH
Reagent A (Rinse), 3.785 L (1 gal) ............................................................ SS-‐035AG
Reagent B (Thiazin Stain), 3.785 L (1 gal) ................................................ SS-‐035/049BG
Reagent A (Rinse) Concentrate, 135 mL (Dilutes to 4.6 L) ...................... SS-‐135/149A SYSTEM
Reagent B (Thiazin Stain) Concentrate, 135 mL (Dilutes to 4.6 L) ........... SS-‐135/149B SYSTEM
Reagent C (Eosin Stain) Concentrate, 135 mL (Dilutes to 4.6 L) ............. SS-‐135/149C SYSTEM
Surfactant (used with SS-‐135 A, B, C) ...................................................... SS-‐135S
Reagent D (Aerofix® Fixative High Humidity), 500 mL ............................ SS-‐048 or SS-‐048-‐EU
Aerofix® Additive for Reagent D (Methanol), 135 mL ............................. SS-‐148 or SS-‐148-‐EU
Reagent A (Basofix™ Rinse), 500 mL ....................................................... SS-‐049A or SS-‐049A-‐EU
Reagent C (Basofix™ Eosin Stain), 500 mL ............................................... SS-‐049C or SS-‐049C-‐EU
Basofix™ Pre-‐dip Fixative, 500 mL ........................................................... SS-‐049P or SS-‐049P-‐EU
Basofix™ Pre-‐dip Concentrate ................................................................. SS-‐149P
Decontamination Solution Concentrate .................................................. SS-‐133
O-‐Ring/Nozzle Thread Grease (3 grams) ................................................. SS-‐103
Preventive Maintenance Chart, pad of 24 sheets ................................... SS-‐265
Aerospray Hematology Stat (Model 7122) Applications Manual ............ RP-‐460
Paper Test Slides ..................................................................................... RP-‐500

94

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Nozzle Tool Unscrews nozzles from the stainer

Nozzle Brush Cleans nozzles without removing

OK Indicates completion of current task.

System Setup Allows users to modify the software settings.

Accessing the System Setup Menu

6 Select the desired Fixation (Off, Normal, High).

2 Select the reagent monitoring options to be modified.

Controlling Beeper Alerts

Performing a Hub Pattern Test

Figure 11: Using Blocking Slides

Table 11: Reagent Level Detect Display Symbols

Waste bottle empty

Emptying the Waste Container

2 Empty the waste container if necessary.

3 At the beginning of the day:

Preparing for Operation after Storage

Decontaminating the Instrument

Decontaminating Reagent Bottles

Figure 13: Correct Slide Pattern Test Result

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