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    BCMB 204 Course Outline

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    The document outlines a course syllabus for an introductory enzymology course. It provides details on course objectives, topics to be covered including enzyme catalysis, kinetics, regulation and applications. It lists learning outcomes and the course schedule over 12 weeks covering these topics. Assessment includes assignments, quizzes and a final exam.

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    BCMB 204 Course Outline

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    The document outlines a course syllabus for an introductory enzymology course. It provides details on course objectives, topics to be covered including enzyme catalysis, kinetics, regulation and applications. It lists learning outcomes and the course schedule over 12 weeks covering these topics. Assessment includes assignments, quizzes and a final exam.

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    The document outlines a course syllabus for an introductory enzymology course. It provides details on course objectives, topics to be covered including enzyme catalysis, kinetics, regulation and applications. It lists learning outcomes and the course schedule over 12 weeks covering these topics. Assessment includes assignments, quizzes and a final exam.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    8 views4 pages

    BCMB 204 Course Outline

    Uploaded by

    DP CHANNEL
    The document outlines a course syllabus for an introductory enzymology course. It provides details on course objectives, topics to be covered including enzyme catalysis, kinetics, regulation and applications. It lists learning outcomes and the course schedule over 12 weeks covering these topics. Assessment includes assignments, quizzes and a final exam.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    UNIVERSITY OF GHANA

    (All rights reserved)


    DEPARTMENT OF BIOCHEMISTRY, CELL AND MOLECULAR BIOLOGY
    SCHOOL OF BIOLOGICAL SCIENCES
    SECOND SEMESTER 2023/2024 ACADEMIC YEAR

    COURSE SYLLABUS
    Course Code and Title: BCMB 204: ENZYMOLOGY
    Credits: Two (2) credits

    Lecture Periods: TBD Venue: TBD

    Lecturer: Prof. Osbourne Quaye / Dr Daniel Oduro-Mensah


    MOB: 027-7459566 / 020-0843252
    Email: [email protected] / [email protected]
    Office Location: Daniel Adzei Bekoe Building: Rooms F 15 / S 14
    Office hours: By appointment
    Teaching Assistants: MOB:

    INTRODUCTION:

    This is an introductory course in enzymology. The course provides an opportunity for students to learn
    the basic concepts necessary to understand how simple catalysts and complex enzymes accelerate and
    regulate biological reactions. Topics covered include enzyme catalysis, mechanism, specificity,
    kinetics, regulation and the purification and application of enzymes in industry, medicine and
    agriculture.

    COURSE DESCRIPTION:

    Introduction to Catalysis: Thermodynamic considerations; Activation energy and transition


    state; Methods for accelerating reactions; Comparison of chemical and enzyme catalysis;
    Features of the active site; Coenzymes; Substrate specificity; Enzyme classification; Catalytic
    mechanisms.

    Enzyme Kinetics: Equilibrium and steady state reactions; Michaelis-Menten model; Graphical
    representation of data (e.g., Lineweaver-Burk and Hanes plots); Significance of Vmax and Km;
    Effect of pH; temperature, and enzyme concentration on reaction rates; Enzyme inhibition:
    Reversible (competitive, uncompetitive, noncompetitive) and irreversible enzyme inhibitors.

    1
    Regulation of Enzyme Activity: Substrate or product regulation (feedback inhibition, feed
    forward activation); Allosteric modulation; Cooperativity; Covalent modification; Isoenzymes;
    Proteolytic activation; Regulation of enzyme synthesis (lac and trp operons),

    Enzyme Purification: Cell disruption techniques, general purification strategy; Assessing


    purity of enzymes; Enzyme assays; Qualitative evaluation of purification.
    Application of enzymes: Industrial application; Medical application; Application;
    Agricultural application; Methods of enzyme immobilization

    GENERAL COURSE OBJECTIVES

    • Understand the importance of enzymes and how they are regulated in cellular reactions.
    • Learn importance of enzymes to man.

    LEARNING OUTCOMES:

    • Students should be able to identify the class of an enzyme catalyzing a biological reaction.
    • Students should be able to predict how temperature, pH, inhibitors, and other factors would
    affect enzyme activity.
    • Students should be able to understand the science and steps in purifying and storing an enzyme.
    • Students should be able to use kinetics as the primary tool to deduce how catalysts work.

    COURSE DELIVERY:

    • Course material will be delivered through lectures and slide projections.

    PLAGIARISM:

    All students are expected to familiarize themselves with the University plagiarism policy. The
    policy is available online at http://www.ug.edu.gh/aqua/policies-guidelines

    COURSE SCHEDULE:

    Dr. D. Oduro-Mensah

    Week 1
    Catalysis

    • Thermodynamic considerations
    • Methods for accelerating reactions
    • Features of enzyme active site

    2
    Week 2
    Catalysis

    • Coenzymes (structures and mechanisms)


    • Classification of enzymes
    Week 3
    Catalytic mechanisms

    • General catalytic mechanisms


    • Catalytic mechanisms of enzymes
    • Specific examples of enzyme-catalysed mechanisms
    • Ribozymes and abyzymes

    Weeks 4, 5 and 6
    Kinetics of Enzyme reactions

    • Rate of one-substrate reactions


    • Equilibrium and steady state reactions
    • Derivation of kinetic parameters
    • The quasi-equilibrium approximation
    • Steady-state approximation
    • Graphical representation of Michealis-Menten equation
    • Linear transformations of the hyperbolic plot
    • Importance of Km and Vmax
    • Effect of pH, temperature, etc

    Prof. Osbourne Quaye

    Weeks 7 and 8
    Enzyme inhibition

    • Irreversible inhibitors and reversible inhibitors


    • Competitive inhibition
    • Noncompetitive inhibition
    • Uncompetitive inhibition

    Weeks 9 and 10
    Regulation of enzyme activity

    • Fine control
    • Substrate or product regulation
    • Allosteric modulation

    3
    • Cooperativity
    • Covalent modification
    • Isozymes
    • Proteolytic activation
    • Coarse control
    • Inducible operon (lac operon)
    • Repressible operon (trp operon)

    Weeks 11 and 12
    Enzyme purification

    • Enzyme extraction
    • Purification strategies
    • Quantitative evaluation of purification steps

    Enzymes and man

    • Industrial application of enzymes


    • Medical application of enzymes
    • Agricultural application of enzymes
    • Enzyme immobilization

    MATERIALS NEEDED FOR COURSE

    • Textbooks of Biochemistry (the most recent editions you can find)


    • Principles of Biochemistry, Lehninger,
    • Biochemistry, Stryer
    • Biochemistry, Voet and Voet
    • Introduction to Enzymology, Adjimani
    • Any other good biochemistry textbook will do.

    • Course material will be delivered through slide projections, and video presentations.

    EXAMINATIONS

    • Interim assessments, assignments and quizzes (35% of final grade)


    • Class attendance (5% of final grade)
    • Final comprehensive exam (60% of final grade)

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