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CIE Biology A-level

Topic 7: Transport in plants

Notes

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Plants require a transport system to ensure that all the cells of a plant receive a sufficient
amount of nutrients. This is achieved through the combined action of xylem tissue which
enables water as well as dissolved minerals to travel up the plant in the passive process of
transpiration, and phloem tissue which enables sugars to reach all parts of the plant in
active the process of translocation.

The vascular bundle

The vascular bundle in the roots:

● Xylem and phloem are components of the vascular bundle, which serves to enable
transport of substances as well as for structural support.
● The xylem vessels are arranged in an X shape in the centre of the vascular bundle.
This enables the plant to withstand various mechanical forces such as pulling.
● The X shape arrangement of xylem vessels is surrounded by endodermis, which is an
outer layer of cells which supply xylem vessels with water.
● An inner layer of meristem cells known as the pericycle

The vascular bundle in the stem:

● Xylem is located on the inside in non-wooded plants to provide support and

flexibility to the stem
● Phloem is found on the outside of the vascular bundle
● There is a layer of cambium in between xylem and phloem, that is meristem cells
which are involved in production of new xylem and phloem tissue

The vascular bundle in the leaf:

● The vascular bundles form the midrib and veins of a leaf

● Dicotyledonous leaves have a network of veins, starting at the midrib and spreading
outwards which are involved in transport and support

Xylem and phloem

Xylem vessels have the following features:

● They transport water and minerals, and also serve to provide structural support
● They are long cylinders made of dead tissue with open ends, therefore they can
form a continuous column.
● Xylem vessels also contain pits which enable water to move sideways between the
vessels.
● They are thickened with a tough substance called lignin, which is deposited in spiral
patterns to enable the plant to remain flexible

The features of phloem vessels include:

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● They’re tubes made of living cells

● Involved in translocation which is the movement of nutrients to storage organs and
growing parts of the plant
● Consist of sieve tube elements and companion cells
● Sieve tube elements form a tube to transport sugars such as sucrose, in the dissolved
form of sap
● Companion cells are involved in ATP production for active processes such as loading
sucrose into sieve tubes
● Cytoplasm of sieve tube elements and companion cells is linked through structures
known as plasmodesmata which are gaps between cell walls which allow
communication and flow of substances such as minerals between the cells

Transpiration
Transpiration is the process where plants absorb water through the roots, which then
moves up through the plant and is released into the atmosphere as water vapour through
pores in the leaves. Carbon dioxide enters, while water and oxygen exit through a leaf’s
stomata.

The transpiration stream, which is the movement of water up the stem enables processes
such as photosynthesis, growth and elongation as it supplies the plant with water which is
necessary for all these processes. Apart from this, the transpiration stream supplies the
plant with the required minerals, whilst enabling it to control its temperature via
evaporation of water.

Transpiration involves osmosis, where water moves from the xylem to the mesophyll cells.
Transpiration also involves evaporation from the surface of mesophyll cells into intercellular
spaces and diffusion of water vapour down a water vapour potential gradient out of the
stomata.

The rate of transpiration can be investigated with the help of a potometer where water lost
by the leaf is replaced by water in the capillary tube. Therefore, measuring the movement of
the meniscus can be used to determine the rate of transpiration. Factors which affect the
rate of transpiration include number of leaves, number/size or position of stomata,
presence of waxy cuticle, the amount of light present, the temperature, humidity, air
movement and water availability.

Xerophytes are plants adapted to living in dry conditions. They are able to survive in such
conditions because of various adaptations which serve to minimise the water loss. The
adaptations include smaller leaves which reduce the surface area for water loss. Both
densely packed mesophyll and thick waxy cuticle prevent water loss via evaporation.
Moreover, xerophytes respond to low water availability by closing the stomata to prevent
water loss. Apart from this, they contain hairs and pits which serve as a means of trapping

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moist air, thus reducing the water vapour potential. Xerophytes also roll the leaves to
reduce the exposure of lower epidermis to the atmosphere, thus trapping air.

Movement of water in the root

Water enters through root hair cells and moves into the xylem tissue located in the centre
of the root. This movement occurs as a result of a water potential gradient, as the water
potential is higher inside the soil than inside the root hair cells, due to the dissolved
substances in the cell sap.

Therefore, the purpose of root hair cells is to provide a large surface area for the movement
of water to occur.

Minerals are also absorbed through the root hair cells by active transport, as they need to
be pumped against the concentration gradient.

There are two ways the water taken up by the root hair cells can move across the cortex
of the root into xylem:

● It can either occur via the symplast pathway where water enters the cytoplasm
through the plasma membrane and passes from one cell to the next through
plasmodesmata, the channels which connect the cytoplasm of one cell to the next.

● The other pathway is the apoplast pathway where the water moves through the
water filled spaces between cellulose molecules in the cell walls. In this pathway,
water doesn’t pass through any plasma membranes therefore it can carry dissolved
mineral ions and salts.

● When the water reaches a part of the root called the endodermis, it encounters a
layer of suberin which is known as the Casparian strip, which cannot be penetrated
by water.

● Therefore, in order for the water to cross the endodermis, the water that has been
moving through the cell walls must now enter the symplast pathway.

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● Once it has moved across the endodermis, the water continues down the water
potential gradient from cell to cell until it reaches a pit in the xylem vessel which is
the entry point of water.

Figure SEQ Figure \* ARABIC 1 Wikipedia - Symplast

Water moving in the xylem up the stem

The water is removed from the top of the xylem vessels into the mesophyll cells down the
water potential gradient. The push of water upwards is aided by the root pressure which is
where the action of the endodermis moving minerals into the xylem by active transport,
drives water into the xylem by osmosis, thus pushing it upwards.

The flow of water is also maintained with the help of surface tension of water and the
attractive forces between water molecules known as cohesion. The action of these two
forces in combination is known as the tension-cohesion theory, which is further supported
by capillary action where the forces involved in cohesion cause the water molecule to
adhere to the walls of xylem, thus pulling water up.

Translocation

Translocation is an energy requiring process which serves as a means of transporting

assimilates such as sucrose in the phloem
between sources which release sucrose such as
leaves and sinks e.g. roots and meristems which
remove sucrose from the phloem.

Sucrose enters the phloem in a process known as

active loading where companion cells use ATP to
transport hydrogen ions into the surrounding
tissue, thus creating a diffusion gradient, which
causes the H+ ions to diffuse back into the
companion cells. It is a form of facilitated
diffusion involving cotransporter proteins which
allows the returning H+ ions to bring sucrose

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molecules into the companion cells, thus causing the concentration of sucrose in the
companion cells to increase. As a result of that, the sucrose diffuses out of the companion
cells down the concentration gradient into the sieve tube elements through links known as
plasmodesmata.

As sucrose enters the sieve tube elements, the water potential inside the tube is reduced,
therefore causing water to enter via osmosis, as a result increasing the hydrostatic pressure
of the sieve tube. Therefore, water moves down the sieve tube from an area of higher
pressure to an area of lower pressure. Eventually, sucrose is removed from the sieve tube
elements by diffusion or active transport into the
Figure SEQ Figure \* ARABIC 2 Boundless
surrounding cells, thus increasing the water
potential in the sieve tube. This in turn means that water leaves the sieve tube by osmosis,
as a result reducing the pressure in the phloem at the sink.

Therefore, in summary, the mass flow of water from the source to the sink down the
hydrostatic pressure gradient is a means of supplying assimilates such as sucrose to where
they are needed.

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