Tissue-Tek Prisma® Plus

Automated Slide Stainer

Operating Manual

Part Number M01-019E-04

Revison Letter B
Revised 2018-02-01

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 Introduction
The automated slide stainer DRS series "Tissue-Tek
Prisma® Plus (hereinafter referred to as the "instrument")"
is an instrument that automatically stains paraffin strips,
frozen strips, cells and other tissues pasted on slides.

Read this Operating Manual carefully so that you can fully

utilize the performance of the instrument based on correct
procedures. Since the instrument uses organic solvents,
exercise due caution when operating the instrument.

Manufactured for:
Sakura Finetek U.S.A., Inc. Torrance, CA 90501, USA
Sakura Finetek Europe B.V., 2408 AV Alphen aan den Rijn, NL
Sakura Finetek Japan Co., Ltd. Tokyo, Japan 103-0023

Manufactured by:
Sakura Seiki Co., Ltd. Nagano, Japan

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 Table of Contents
Safety Precautions ................................................................................................................................... (e)
Explanation of Terms................................................................................................................................ (p)

Chapter 1 Basics of the System

Installation Method .................................................................................................................................. 1-1
Installing the Instrument ...................................................................................................................... 1-1
Flow of Installation ........................................................................................................................... 1-1
Required Tools ................................................................................................................................. 1-1
Installation Environment .................................................................................................................. 1-1
Checking the Installation Conditions ................................................................................................... 1-2
Cecking the Work Area ........................................................................................................................ 1-3
Transportation ..................................................................................................................................... 1-3
Unpacking Procedure .......................................................................................................................... 1-4
Unpacking the System ..................................................................................................................... 1-4
Removing the Securing Members Inside the Instrument ................................................................ 1-6
Switching the Power-supply Voltage Setting ....................................................................................... 1-7
Checking the Accessories ................................................................................................................... 1-7
Checking the Installation (Securing the Instrument) ........................................................................... 1-9
Installing the parts around the instrument ........................................................................................... 1-9
Installation of Accessories ................................................................................................................. 1-14
Turning on the Power ........................................................................................................................ 1-16
Specification .......................................................................................................................................... 1-17
Name of Each Part ................................................................................................................................ 1-20
Standard Accessories/Options .............................................................................................................. 1-22
Basics .................................................................................................................................................... 1-24
Basics on This Instrument ................................................................................................................. 1-24
Instrument Overview ...................................................................................................................... 1-24
Stain Process Modes ..................................................................................................................... 1-24
User Management by Password ................................................................................................... 1-24
Logon/Logoff Function ................................................................................................................... 1-25
Safety Mechanism of the Instrument ............................................................................................. 1-25
How to Open and Close the Cover/Door ....................................................................................... 1-25
Handling the Touch-panel Display ................................................................................................. 1-26
Various Gateways .......................................................................................................................... 1-27
Relationship of Solution Configurations and Station Numbers ...................................................... 1-29
Explanation of Menu Screens ............................................................................................................... 1-32
Stain Process Menu .......................................................................................................................... 1-32
Explanation of Icons in the Stain Process Menu ........................................................................... 1-33
Utility Menu ........................................................................................................................................ 1-34

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 Explanation of Icons in the Utility Menu ....................................................................................... 1-34
Edit Menu ......................................................................................................................................... 1-35
Explanation of Icons in the Edit Menu ........................................................................................... 1-35
System Setup ........................................................................................................................................ 1-36
System Setup .................................................................................................................................... 1-36
Setting Date and Time ....................................................................................................................... 1-38
Entering Year ................................................................................................................................. 1-38
Entering Month .............................................................................................................................. 1-38
Entering Day .................................................................................................................................. 1-38
Entering Hours and Minutes .......................................................................................................... 1-38
am/pm ............................................................................................................................................ 1-38
Setting User IDs, Passwords and Accessible Functions ................................................................... 1-39
Setting an ID .................................................................................................................................. 1-39
Setting a Password ........................................................................................................................ 1-39
Setting Accessible Functions ......................................................................................................... 1-40
Stain Process ................................................................................................................................. 1-40
Utility .............................................................................................................................................. 1-40
Edit ................................................................................................................................................. 1-40
Fume Filter Management .................................................................................................................. 1-41
Whether or Not to Use Fume Filter Management.......................................................................... 1-41
Setting a Time Limit ....................................................................................................................... 1-41
Resetting the Actual Time .............................................................................................................. 1-41
Adjusting the Water Flow................................................................................................................... 1-42
Export Data ........................................................................................................................................... 1-43
Names of Files Exported to a CF Card .......................................................................................... 1-44

Chapter 2 Operating Procedure

Operating Procedure ............................................................................................................................... 2-1
Flow of Stain Process Operation ......................................................................................................... 2-1
Flow of Stain Process Operation ..................................................................................................... 2-1
Preparation for Stain Process ............................................................................................................. 2-2
Opening the Water Supply ............................................................................................................... 2-2
Logging On to the Instrument .......................................................................................................... 2-2
Selecting and Saving a Solution Configuration and Setting Solutions ................................................ 2-3
Setting Slides into Baskets .................................................................................................................. 2-4
Start of Stain Process .......................................................................................................................... 2-5
Operations Permitted during Stain Process ........................................................................................ 2-7
Adding a Basket ............................................................................................................................... 2-7
Setting Priority Start ......................................................................................................................... 2-8
Checking the End Station Status ..................................................................................................... 2-8
Checking the Staining Status .............................................................................................................. 2-9
Menu Operations Permitted during Staining Process ................................................................... 2-10
Pausing and Aborting Stain Process ............................................................................................. 2-11
Removing a Basket during Stain Process ..................................................................................... 2-12

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 End of Stain Process ......................................................................................................................... 2-12
Removing Stained Baskets............................................................................................................ 2-12
End of Operation ............................................................................................................................... 2-12
How to Start Stain Process by Specifying the Start and End Steps ................................................. 2-13
Setting a Solution Configuration ........................................................................................................... 2-15
Creating a New Solution Configuration ............................................................................................. 2-15
Saving a Staining Program Name ................................................................................................. 2-15
Setting Up a Solution Configuration .............................................................................................. 2-16
Setting an Edit Solution Configuration ........................................................................................... 2-17
Mix Parameter Setup ..................................................................................................................... 2-20
Enhanced Wash Parameter Setup ................................................................................................ 2-20
Basket Lifting Speed and Water Wash Cycle Time Parameter Setup ........................................... 2-21
Temperature Settings ..................................................................................................................... 2-21
Editing a Solution Configuration ........................................................................................................ 2-22
Copying a Solution Configuration ...................................................................................................... 2-22
Editing a Solution Name .................................................................................................................... 2-23
Creating and Editing a Solution Name .......................................................................................... 2-23
Copying a Solution Name .............................................................................................................. 2-24
Setting a Staining Program ................................................................................................................... 2-25
Creating a New Staining Program ..................................................................................................... 2-25
Saving a Staining Program Name ................................................................................................. 2-25
Creating a Staining Program ......................................................................................................... 2-26
Editing a Staining Program ................................................................................................................ 2-31
Copying a Staining Program ............................................................................................................. 2-32
Checking a Staining Program............................................................................................................ 2-33
How to Use the Bar Code Reader, and It's Function (Options) ............................................................ 2-34
Overview............................................................................................................................................ 2-34
Applicable Solutions .......................................................................................................................... 2-34
How to Use Sakura Staining Solutions ............................................................................................. 2-34
Editing a Solution Configuration .................................................................................................... 2-34
How to Enter Sakura Solution Bar Code Data............................................................................... 2-35
How to Use Sakura Staining Kit ........................................................................................................ 2-36
Editing a Solution Configuration .................................................................................................... 2-36
How to Enter Kit Component Solution Bar Code Data .................................................................. 2-37
Resetting the Solution Usage Information ......................................................................................... 2-39
Outputting Process Report ................................................................................................................ 2-40
Solution Usage Information Report ............................................................................................... 2-40
Process Report .............................................................................................................................. 2-41
Solution Configuration Report........................................................................................................ 2-42
Barcode History Report ................................................................................................................. 2-43
Deleting a Solution Configuration/Staining Program/Solution Name/Process Report ...................... 2-44
Deleting a Solution Configuration .................................................................................................. 2-44
Deleting a Staining Program .......................................................................................................... 2-45
Deleting a Solution Name .............................................................................................................. 2-45
Deleting a Process Report ............................................................................................................. 2-45

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 Action to Be Taken upon Power Outage ............................................................................................... 2-46
Power Recovery Operation ............................................................................................................... 2-46
Action to Be Taken upon Error .............................................................................................................. 2-47
Error Handling ................................................................................................................................... 2-47
Errors with a Number ..................................................................................................................... 2-47
Other Errors ................................................................................................................................... 2-47

Chapter 3 Maintenance and Inspection

Daily Inspection and Cleaning ................................................................................................................ 3-1
Inspection and Cleaning Methods ....................................................................................................... 3-1
Cleaning the Solution Reservoirs and Baskets ............................................................................... 3-1
Cleaning the Drying Stations ........................................................................................................... 3-1
Cleaning the Heating Stations ......................................................................................................... 3-1
Replacing Activated Carbon Filters ................................................................................................. 3-2
Cleaning the Arm Holder and Basket Adapter ................................................................................. 3-2
Cleaning the Water Supply Strainer ................................................................................................ 3-3
Cleaning the Cabinet ....................................................................................................................... 3-3
Cleaning the Wash Station Nozzle .................................................................................................. 3-4
Cleaning the Drain Hose .................................................................................................................. 3-4

Chapter 4 Troubleshooting
Troubleshooting ...................................................................................................................................... 4-1
Error Codes Displayed during System Operation ............................................................................... 4-1
Instrument Conditions and Recovery Actions ..................................................................................... 4-7

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 Safety Precautions
Designation of "Instrument Control Manager"
● Operation of the instrument requires expert knowledge of the target application, method of use, and so
on. So that the instrument can be used correctly and safely, designate a "Instrument Control Manager."
● When the instrument is delivered, the Instrument Control Manager should receive explanation on the
handling of the instrument directly from our sales representative.

* Before using the instrument, read "Safety Precautions" to ensure the correct use of the instrument.
* The cautionary instructions provided herein are intended to ensure that the instrument will be used
safely in a manner preventing operator from injury and property damage.
* In this manual, instructions pertaining to different levels of potential hazards are classified as and
indicated under , , and .

The definition of each hazard is defined below.

pertains to a potential hazard where failure to observe the specified instruction may result in death or
serious injury of the operator or other person.

pertains to a potential hazard where failure to observe the specified instruction may result in a serious
injury of the operator or other person.

pertains to a potential hazard where failure to observe the specified instruction may result in damage to
this instrument or other property, or affect the process results.

pertains to an item to pay attention to or other useful information.

* The symbols used on the labels attached to the instrument are explained.
The labels bearing one of the following symbols provide particularly important information you must
know in order to ensure safety of the operator, improve work efficiency and protect the instrument from
damage. Be sure to check these labels and understand the specified instructions before commencing
your work.

A label bearing this symbol specifies an action that must be taken. Always follow the instructions.

A label bearing this symbol specifies a prohibited item. Always follow the instructions.

A label bearing this symbol specifies a precaution. Wrong handling against the instruction may
potentially expose the operator to danger or result in damage to the instrument. Always follow
the instructions.

A label bearing this symbol is provided near a high-temperature area. Exercise caution against
burns caused by high temperatures.

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 Safety Precautions
WARNING

● Read the unpacking instructions carefully before unpacking the instrument.

When removing the instrument from the pallet, two or more persons should work together because
the instrument weighs 150 kg. When removing the instrument from the pallet this way, indicate so in
the shipping document and inspect the cargo for damage. As a rule, the instrument is
unpacked/installed by Sakura Finetek or our local representative.

● Those who have experience operating a Sakura Finetek product or products other than the
instrument must also read this manual. Also, those who will be operating the instrument
should receive training first.
Sakura Finetek will assume no responsibility for any accident that may result from an accident
caused by operating the instrument without reading this manual first. If the instrument is operated in
a manner not specified in this document, the protective functions of the equipment may be lost.

● Do not apply unnecessary external forces on the instrument and accessories.

The instrument may malfunction or cause accidents.

● Avoid connecting the instrument to a transformer not manufactured by Sakura Finetek.

When moving the instrument to a region where different voltage is used, contact the Sakura Finetek
Technical Support representative or local representative.

● Safety mechanisms installed in the instrument only represent the minimum measures taken
to prevent accidents. The responsibility for safe operation of the instrument lies primarily
with the owner of the instrument, and persons in charge who operate the instrument.

● Do not use the instrument, if damaged, even when operating it is possible.

Injury or electric shock may result.

● Should the instrument malfunction, turn off the power and disconnect the power plug from
the power outlet. If processing is still in progress, immediately protect the tissues.
Should malfunction occur, turn off the power and contact the Sakura Finetek Technical Support
representative or local representative. Disconnecting the power plug also cuts off the main power
supply. When installing the instrument, select a place where the power outlet is not concealed and
the power plug can be disconnected any time in case of emergency.

● Do not disassemble or modify the instrument.

The instrument may malfunction or cause accident, thus creating a dangerous situation.

● Only the specified solutions can be used with this instrument.

If any other solution is used, processing may not be performed properly and fire or failure may
result.

● Do not wet the instrument.

If areas other than those instructed by "Daily Inspection and Care" get wet, current leak may occur
and cause fire or electric shock. If wetted, the board, power switch, CF card and CF card slot,
connection parts of various terminals art located on the right side of the instrument may cause
instrument failure or data damage.
If the terminal connection part LAN or bar code terminal is not used, put a protective cap over it.

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 Safety Precautions

WARNING
● A wrong operation may stop or interrupt the tissue processing.
Be careful not to let unauthorized personnel manipulate or tamper with the instrument while
operating.
A wrong operation may stop or interrupt the tissue processing. (If the power is turned off by
unauthorized personnel, the stain process may be interrupted and the staining problems may occur.
Arrange the work environment so that only the operators with expert knowledge can use the
instrument. (Also consider using external alarms, etc., just in case.)

● Do not bring fire sources close to the instrument.

The instrument uses organic solvents that may ignite upon contact with fire.

● Do not let foreign matter go inside the instrument.

If water, metal, paper or other foreign matter enters the air inlets (to the fan, etc.), fire, electric shock,
injury of the user, instrument failure, etc., may occur. If foreign matter entered the instrument,
immediately stop the instrument and contact the Sakura Finetek Technical Support representative or
local representative.

● Do not operate the instrument while wearing metal accessories.

Conductors (metal pen, accessories, etc.) worn on your body may contact the instrument and cause
electric shock.

● Do not block the intake/exhaust ports and air inlet around the instrument.
Do not block the intake/exhaust ports (air inlets) with a wall or object or use the instrument in a poorly
ventilated place or with the dustproof covers still attached, because heat will be trapped in the
instrument and cause the capacity of the instrument to drop, while fire, accident, failure, etc., may also
occur due to abnormal overheating.

● Periodically check the power cord and cables and power outlet for damage and accumulation
of dust.
Foreign particle and dust accumulating on the power outlet may cause fire. Clean the power outlet
periodically.

● Do not let tissue with flammable solvent attached go inside the drying station.
Deposited flammable solvent may be heated by the drying station and vaporize, causing fire or
explosion.

● Do not install the instrument in a place used as a living area.

If a common area is used, an exhaust system is required. Provide electrostatic discharge protection
in the installation location.

● Install the instrument indoors. Do not place the instrument in a location exposed to direct
sunlight, snow or rain.
This product is specified for indoor use, so do not use it outdoors. Ultraviolet rays and high
temperature may damage the instrument. Never use the instrument while exposing it to snow or rain
because it may cause electric shock or short-circuit.

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 Safety Precautions

WARNING
● Unplug the power cord from the power outlet and store it while the instrument is not in use.
For safety, if the instrument will not be used for an extended period of time, clean each part
and store the instrument in a place free from humidity and from dust and foreign matter.
Using the instrument in a humid place, place exposed to oil smoke or steam, or dusty place, may
cause its parts to deteriorate, short-circuit, fire, etc. The installation humidity range is 30 to 85% (non-
condensing). If you wish to install the instrument in a special place, consult the Sakura Finetek
Technical Support representative or local representative.

● If dews are formed on the instrument, do not turn on the power.

If the power is turned on while dews are formed on the instrument, current leak may occur and cause
fire or electric shock. Do not connect the instrument to a power-supply facility until dews have
evaporated and the instrument has become completely dry.

● Do not operate the power plug and switches with a wet hand. When connecting/ removing the
power plug, do so by holding the plug.
Failure to observe these instructions may result in electric shock .

● Be sure to use the supplied power cord. Also, be sure to use a correct power cord that is
suitable for the current capacity of region where the instrument is used.
If an inappropriate power cord or voltage is used, instrument malfunction due to short-circuit, fire or
voltage drop, unexpected accidents or injuries due to electric shock, etc., can occur. The preset
voltage cannot be changed by the user.

● For the power cord, a standard cord specified by local law may have to be used.
If a power cord not specified by the PSE standard, UL/CSA standard or EC standard is required,
contact the Sakura Finetek Technical Support representative or local representative.

● Do not forcibly bend, pull, twist or bundle the power cord and cables or otherwise damage the
power cord and cables.
Fire or electric shock may occur.

● Do not overload the outlet and do not use an extension cord.

Doing so may stop the operation of the instrument due to voltage drop, or fire can occur due to heat
generation.

● This instrument has been designed and tested according to CISPR11 Class A. When the
instrument is used in a home, it may cause electromagnetic interference, in which case
measures to reduce interference may have to be taken.

● Radiant waves may prevent this instrument from operating properly, so do not use the
instrument near a radiation source of strong electromagnetic waves.

● Use the instrument under the conditions (such as voltage within 10% of the rated voltage)
stated in the Specification.

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 Safety Precautions

CAUTION
● Do not pinch the drain hose or allow it to slacken.
Water will not be drained properly and overflow from the instrument.

● Set the solution station (tray) in the correct location.

If not, the tissues may not be soaked in the solution and staining problems may occur.

● Store the slides evenly in the basket.

If the slides are stored unevenly, basket movements are affected during the staining operation.

● Before staining is started, confirm that the end station has been set.
If the end station is not set, the basket cannot be put down and will remain gripped. If the next basket
is processed in this condition, the baskets may collide with each other and the tissues may be
damaged.

● Do not touch the moving arm.

The arm should stop automatically when the cover is opened, but it may continue to operate if a
series of operations is still ongoing. Touching the moving arm may result in injury. If you must move
the arm by hand, confirm that the arm has stopped completely and then move the arm with due
caution by following the onscreen messages on the display.

● Do not open the cover or door unless necessary.

Open the cover or door only when required by the operations explained in this manual. If the cover or
door remains open for a long period of time, organic gas may be released from the instrument. Also,
opening the cover or door during staining stops the arm, which may cause the tissues to dry up or
extend the staining time and eventually affect the stained quality of tissues.

● Be careful not to pinch your hand when opening/closing the cover or door.
Be careful not to pinch your hand between the fixed part and moving part of the cover or door or
between the cover and door.

● Do not move the instrument while operating. Move the instrument after removing all solution
bottles, paraffin containers and other components carrying liquid inside.
Moving the instrument while operating may cause the solution or paraffin to leak. Accident or failure
may also occur.

● If the straining process is suspended, the process may be negatively affected depending on
the situation. Remember to resume operation.
Prolonged suspension may negatively affect the stain process. Keep the suspension time to the
minimum.

● When the expiration date of the current solution is passed, a message appears to urge
exchanging the solution. Exchange the solution properly in order to maintain the staining
quality.

● The safety data sheet of each solution used can be obtained from the supplier/manufacturer of
the solution. You can also download it from the website of respective supplier/manufacturer of
the solution.

● Do not insert a metal bar or other metal object into the hot air outlet of any drying station or
use a metal product to reset an over temperature protector.
Metal contact may cause electric shock.

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 Safety Precautions
CAUTION
● Do not allow any foreign object to be caught between the cover/door and the stainer.
It may cause an unexpected problem.

● Use tap water for the water supply.

Use of water containing pollutants may result in staining problems.

● Confirm that no paper or foreign object is present at the cover or outlet of any drying station.
It may result in insufficient drying.

● Do not touch the drying stations and heating stations while the instrument is operating.
These stations become very hot during operation, so touching them may result in burns.

● Securely connect the water supply hose and drain hose to make sure water will not leak.
If water leaks, the facility may be damaged.

● Before using the instrument, inspect the water supply hose and drain hose to ensure that they
are free from damage and connected properly.
It may cause an unexpected problem.

● Close the water tap after using the instrument.

If the water tap remains open, the water supply hose may accidentally come off due to water
pressure and cause water leaks.

● Clean the water supply strainer regularly. Also, close the supply source water tap before
cleaning.
If the water supply strainer is not cleaned regularly, the water supply volume will drop and washing
problems may occur. Check the flow rate after cleaning. If the water supply strainer is cleaned with
the supply source tap open, water will overflow when the strainer is removed.

● Clean the drying station regularly.

If the drying solution is not cleaned regularly, paraffin will accumulate in the pan and cause the
temperature to vary, resulting in staining problems.

● Clean the water supply nozzle regularly.

If the water supply nozzle is not cleaned regularly, contaminants may attach to the nozzle and enter
the tissues, causing staining problems.

● Clean the instrument thoroughly after using corrosive solutions.

Some solutions have corrosivity and other aggressive properties that damage the instrument. It is
best to avoid using these corrosive solutions, but if you must use a corrosive solution, remove the
solution from the instrument and store it properly after use and also clean the instrument thoroughly. If
this is not performed, the metal parts will corrode (rust, etc.).

[Corrosive Solutions]
Acids [hydrochloric acid (HCl), hydrobromic acid (HBr), hydrofluoric acid (HF), etc.], staining solutions
and reagent solutions containing hydrochloric acid (Schiff’s reagent, cold Schiff, sulfurous acid
solution, resorcin‐fuchsin staining solution, etc.), oxidizing chlorides [ferric chloride (FeCl3), mercuric
chloride (HgCl2), cupric chloride (CuCl2), sodium hypochloride (NaOCl), etc.], tioculfates [sodium
tiosulfate (Na2S2O3), etc.], organic acids [formic acid (HCOOH), acetic acid (CH3COOH), bromic
acid (C204H2), lactic acid (CH3CH(OH)COOH), etc.]

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 Safety Precautions
CAUTION
● When the pull-up speed is increased, more solution will be brought into the next station and
accelerate solution deterioration, resulting in lower staining quality. There are also risks of
tissue contamination due to dropping solution.

● Do not stare at the laser beam from the 2D bar code reader.
For details, refer to the operating manual. The operating manual can be downloaded from the website
of the 2D bar code reader manufacturer.

● Observe the rated water pressure stated in the Specification of the instrument.
If the water pressure is high, water may leak and cause electric shock or the instrument may stop.

● Do not use any CF card other than the one that comes with the instrument. Also, run a virus
check before inserting the CF card.

● Turn off the power before performing cleaning.

Unless otherwise instructed in this manual, always turn off the power and disconnect the power plug
before cleaning the instrument. Do not spill liquid inside the instrument while cleaning it. If cleaner is
used, follow the safety precautions provided by its manufacturer and safety rules set by each test
laboratory.

● Secure the instrument with seismic anchorage (optional) to prevent it from moving/falling in
case of earthquake.
Provide fall prevention measure to prevent the instrument from moving should a disaster occur.

● Do not apply excessive force on the display.

The display may break and cause injury.

● Do not remove the warning/caution labels.

Without these labels, the operators cannot be reminded of the necessary warning/cautionary
information in the day-to-day handling of the instrument, which may result in unexpected problems.

● Do not use damaged accessories (reservoirs, baskets, basket adapters, etc.).

The accessories deteriorate from use over time. If deteriorated accessories are used, unexpected
problems may occur. Check the accessories before use.

● Do not put solution, etc., on the instrument. Also, do not spill solution on the painted surfaces,
caution/warning labels or touch screen.
If solution is spilled over the instrument, the instrument may fail or sustain damage.

● Do not spill solution inside or outside the instrument.

If solution was spilled, turn off the power to the instrument and then quickly wipe off the spilled
solution. The instrument may stop or the facility may be damaged, etc. Also, other solution may be
contaminated. Do not use any contaminated solution.

● Put the lids on other stations when exchanging solution.

If solution is spilled during exchange, other stations may be contaminated. Also, do not use, but
always replace, any contaminated station.

● Do not put any obstacle within the moving range of the arm.
Failure may occur.

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 Safety Precautions
CAUTION
● Do not clean the solution reservoirs and baskets using hot water or heat the solution
reservoirs containing solutions in a microwave oven.
Heating the solution reservoirs and baskets using hot water or in a microwave oven may cause the
reservoirs/baskets to deform. Severely deformed baskets and solution reservoirs may rub against
each other on side faces or at corners, thereby inhibiting the operation.

● Do not use any ultraviolet sterilizing lamp around the instrument.

Ultraviolet light may cause the instrument characteristics to change or deteriorate.

● Fill each solution reservoir with a specified amount of solution.

Failure to do so may cause tissues to dry up or result in staining problems.

● Replace the activated carbon filters in the fume control unit periodically.
Replace the activated carbon filters regularly in order to maintain a better environment around the
instrument. Expired activated carbon filters cannot maintain exhaust gas at an appropriate level of
concentration. When replacing the activated carbon filters, put the lids on the solution stations or
remove the solution stations by considering the possible scattering of activated carbon particles, to
prevent solution contamination.
* If the instrument is connected to an external exhaust device, the activated carbon filters need not be
replaced.

● Use dedicated activated carbon filters for the fume control unit.
Use of inappropriate activated carbon filters may cause explosion of powder dust generated from
activated carbon powder.

● Inspect the instrument every 6 months.

Maintain/inspect the instrument periodically so that it operates correctly at all times. For details on
periodic inspection, contact the Sakura Finetek sales representative.

● If the instrument must be moved after installation, contact the Sakura Finetek Technical
Support representative or local representative.
Unexpected accident may occur by relocating the instrument, so contact the Sakura Finetek Technical
Support representative or local representative.

● Use designated consumable parts.

When replacing consumable parts, use those specified in this manual. Use of any other consumable
part may result in malfunction or failure.

● Close the lids of solution reservoirs whenever the instrument is not operating.
If the solution reservoir lids remain open for a prolonged period of time, organic gas may be released
to the room.

● Provide sufficient ventilation around the instrument.

Some of the solutions used in the instrument are toxic/harmful to the human body. Accordingly,
provide sufficient ventilation around the instrument.

● Install the instrument away from other electrical equipment.

Electromagnetic waves and vibration, heat and solution may affect and be affected by other systems
located nearby.

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 Safety Precautions
CAUTION
● Provide a proper space around the instrument.
Provide at least 10 cm of clearance around the instrument and make sure the power plug is installed
so that it can be accessed at any time.

● Thoroughly check the installation location before installing the instrument.

Install the instrument on a flat, non-slippery sturdy floor having a sufficient size. If the installation
location is tilted or too small, unexpected accidents may occur.

● Do not use the instrument in a place subject to too low or high temperatures.
Use it in a place where the temperature is stable in a range of 10C to 40C.

● Be sure to connect the power plug to a power outlet with grounding bar.
Connect the power plug alone to a power outlet with grounding bar conforming to at least the Class D
grounding specification. If the instrument is not grounded properly, fire or electric shock may result
due to leak current.

● Lightning and power outage may cause the instrument to fail and stain process may not be
able to continue and adversely affect staining. If the power supply is unstable, use an
uninterruptible power supply.
Lightning may cause the instrument to fail and damage the tissues. If the instrument will be used in a
region where lightning damage is a possibility, use an uninterruptible power supply. If lightning has
caused an irreparable damage to the instrument, take out the basket from the instrument and take an
appropriate measure.

● Do not perform stain process if an extended period of power outage is scheduled. Staining
quality may drop. Staining problems may occur depending on when the power outage occurs.
In this case, use a uninterruptible power supply. If you find that the stain process has stopped due to
an unexpected power outage lasting for an extended period of time, remove the baskets from the
instrument and take appropriate action.

● Install the power cord and cables carefully by making sure the cord and cables will not
interfere with the work.
Failure to do so may result in the operator tripping over the power cord and cables and sustaining
injury.

● Securely connect the power cord and power outlet. If the power cord came off or the power
switch was turned off inadvertently, connect the power cord or turn on the power switch
without delay.
If they are loosely connected, heat may be generated at the connected part, voltage drop can occur
and the instrument may not operate normally. Furthermore, unexpected accidents or injuries can
occur. Also, the tissues may be damaged when the power is cut off during processing.

● Handle the power cord with care. Also, be aware of damage caused by mice and other small
animals.
To unplug the power cord from the power outlet, do not pull the cord, but hold the plug and then pull it
out. Inspect the power cord periodically. If any damage is found in the exterior of the power cord, stop
using it and contact the Sakura Finetek Technical Support representative or local representative.

● Before operating the instrument, be sure to check the electromagnetic environment.

Example: Remove electrostatics from the operator and humidify the room.

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 Safety Precautions
CAUTION
● If an error (trouble) occurs, staining problems may occur. Immediately resolve the error,
protect the tissues, and contact the Sakura Finetek Technical Support representative or local
representative.
Connect an external alarm to prevent staining problems from happening when the instrument
experiences problems. When introducing an external alarm, contact the Sakura Finetek Technical
Support representative or local representative.

● Conduct a staining test before determining the staining conditions.

● Check the staining program before the operation. If a wrong staining program is used, staining
problems may occur.
A wrong operation may interrupt the stain process or edit the program to a wrong one. If staining is
started with a wrong program, staining problems may occur. Read this manual carefully and use the
instrument correctly.

● Operating the instrument with an inappropriate temperature setting or time setting (long/short)
may cause staining problems. Conduct a verification test beforehand to confirm that staining
can be performed properly. If you have any question regarding staining programs, contact the
Sakura Finetek Technical Support representative or local representative.

● The allowable concentration limit of each substance in the work environment varies
depending on the air temperature, size of the room, additional load, if any, ventilation efficient
y, etc.
If danger is suspected, the Instrument Control Manager should measure the concentration of the
applicable substance in the area and confirm that the allowable limit in the work environment is not
exceeded. Follow the standard concentration limits specified by the environmental regulation of the
country/region in which the instrument is used.

● Solution may scatter and attach to your body while working. Be sure to wear protective gears
while working.
In all operations including stain process, solution exchange, opening/closing of doors, cleaning and
daily maintenance, the solutions used may scatter and attach to your body (eye, mucous membrane,
skin, mouth, nose, etc.) or you may inhale their gases. Be sure to wear protective gears (gloves,
organic solvent mask, goggles) while working.

● If any solution harmful to human health attached to your body or entered your mouth, take an
appropriate action according to the SDS of the solution.
Wear protective gears (mask, gloves, goggles) during work in case anything goes wrong. Use
protective gears that are resistant to all solutions on the solution list.

● You may accidentally touch an infectious source and operate the instrument with the
contaminated hands. Be sure to wear protective gears when operating the instrument.
If you have operated the instrument after handling a pathogen, disinfect the instrument properly. The
disinfection method varies depending on the type of pathogen, so choose a method appropriate for
the pathogen. Also, disinfect/sterilize or otherwise properly treat the protective gears used and then
dispose of them as infectious waste. For the disposal method, follow the applicable regulation and
guideline specified by each institution, country or region.

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 Safety Precautions
CAUTION
● Exercise due caution when handling solutions. Follow the relevant regulations and guidelines
in your country or region and take proper measures to protect the operator, such as wearing
gloves, mask and/or safety goggles.
Some solutions are toxic or harmful to the human body. Some solutions are very flammable,
combustible, and/or harmful to human body. Use protective gears that are resistant to all solutions on
the solution list.

● Sterilize any potentially infectious waste material or infectious medical waste and then
dispose of it according to the applicable regulation or guideline in the country or region.

● When the instrument is used to handle any substance presenting biological hazard, ensure
safety according to the applicable regulation or guideline in the country or region. When
performing operations that involve any such substance, also wear gloves, mask, safety
goggles and/or other protective gears and perform the operations inside a safety cabinet by
taking appropriate measures to protect yourself as the operator.

● Handling of solutions requires expert knowledge on associated hazards due to their toxicity
and volatility, etc.
Appoint a person responsible for solutions and use solutions under the guidance/supervision of the
responsible person.

● If any additional requirement on accident prevention or environmental preservation is

specified in the country/region where this instrument is used, follow the instructions under the
applicable law/regulation in addition to the content of this manual to ensure compliance with
the requirement in the applicable country/region.

● When disposing of used solutions, activated carbon cartridges, consumable accessories

(solution reservoirs, baskets, basket adapters, etc.), follow the applicable regulation in the
country or region.

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 Explanation of Terms
● Mix
To cause a basket to move up and down inside a solution-filled station before or after the basket is
transferred.

● Enhanced Wash
To repeat the process of immersing a basket into a solution and then removing it completely out of the
solution inside a station.

● Slide
A slide made of glass on which tissues are pasted.

● Basket
A special staining basket in which slides are set. Two types of baskets, 10-slide type and 20-slide type,
are available.

● Batch mode
An operation mode in which the next staining run cannot be started unless the current staining run is
completed.

● Continuous mode
An operation mode in which staining runs of different tissues can be started during the current staining
run.

● Solution station
A general term referring to both solution reservoirs and wash reservoirs.

● Start station
A solution station in which baskets are set at the start of staining.

● End station
A solution station in which stained baskets are stored.

● Program End (PE) station

A solution station in which baskets are temporarily held when the staining program is aborted in the
middle.

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 Chapter 1
Basics of the System

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 Installation Method
Installing the Instrument
This section provides information on determining a location for, and installing the instrument.
Installation should be performed by qualified service staff working exclusively with the instrument.
The instrument must be installed correctly to ensure proper operation and service.
Read this Operating Manual carefully before operating the instrument. Strictly observe all instructions
provided in this document.

This is a precision instrument and must be handled with care. If the instrument is handled in a rough
manner or dropped, its internal components may malfunction or get damaged. Always exercise due
care when handling the instrument.

Flow of Installation

 Checking the Installation Location and Transporting the Instrument Details: P. 1-2
• Checking the installation conditions
• Checking the work area
• Transporting the instrument

● Unpacking and Checking the Installation Details: P. 1-4

• Unpacking and installation
• Installation check

● Setting before Commencing Operation Details: P. 1-16

• Turning on the power

Required Tools

Name Size Q'ty

Transport handle - 4
Phillips screwdriver ISO No. 2 1
Adjustable wrench 300 mm or more 1
Wrench or box wrench 24 mm across flats 2
Convex ruler (steel tape
Capable of measuring objects of 2.5 m or larger 1
measure)
Level - 1
Cutter - 1
Pipe wrench 200 mm or more 1

Installation Environment
As is the case with all electronic devices, the instrument should not be installed in places subject to
excessive temperature or humidity.
Temperature and humidity should be held at constant levels to achieve the highest degree of operating
stability. The ambient operating temperature of the instrument is in a range of +10 to +40C. Its ambient
operating humidity is in a range of 30 to 85% in relative humidity (non-condensing).
Also, locate the instrument, with at least 10 cm away from walls, in a well-ventilated place not exposed to
corrosive vapor or extremely high/low temperatures or where the instrument will not come in direct contact
with air flow. Install the instrument away from direct sunlight and do not install it proximity to open
windows, ovens, open flames, hot plates, radiators, and dry ice baths. The instrument should be installed
away from any equipment carrying high voltage or current, such as a large refrigerator or oven. Since the
instrument is very heavy, the experiment bench should have sufficient bearing strength, and the
instrument should be laid flat.
The instrument should be installed near a power supply meeting the power facility requirements (voltage,
current) specified in this Operating Manual. Be sure to ground the power outlet and wire the power
properly using a dedicated cable.

If the instrument is used in a non-ventilated place, serious health risks may occur to the human body.

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 Installation Method
Checking the Installation Conditions

 Checking the installation

location
Confirm beforehand that the
location is flat, strong and meets
the dimensions of installation
location (installation space) shown
in the figure on the left.
Confirm that no foreign objects that
may block the vents of the
instrument (such as hanging
towels, etc.) are present in and
around the installation location.
Confirm that the facility-side power
supply conforms to the
requirements.

 Checking the facility-side power supply

Confirm that the facility-side power supply conforms to the requirements.

The instrument can support the power supplies listed below using the selector switches.
Switching of the voltage setting should only be performed by qualified service staff working
exclusively with the instrument.

Facility requirements
• Single-phase 100 VAC±10% 50/60 Hz: 15 A or more Power outlet with grounding bar
• Single-phase 115 VAC±10% 50/60 Hz: 15 A or more Power outlet with grounding bar
• Single-phase 230 VAC±10% 50/60 Hz: 7 A or more Power outlet with grounding bar

If the above facility requirements are not satisfied (such as when the power supply used is 200 VAC),
the instrument must not be used.
If the instrument generates abnormality, the breaker on the facility side may actuate and other
equipment may be affected; accordingly, connect each instrument to a dedicated power outlet
(including a dedicated breaker on the facility side).

• Specification of the power cord supplied with the instrument

Type of power cord Applicable standard Length
For 100/115 VAC UL/CSA, PSE Approx. 2 m
For 230 VAC Cord: CENELEC HD VDE Approx. 2 m
Plug: EN IEC VDE
Connector: EN VDE

If the instrument is used in a region where the applicable standard above is not met, provide a different
power cord conforming to the standard in the location of use.

• About grounding
Confirm with the person responsible for the facility that the power outlet to be used is Class-D grounded.

 Confirm that a ventilation system, local exhaust system or exhaust port leading to the outside
of the building is available on the facility side.
Although the volumes of volatile solution gases released from the instrument vary depending on the
solutions and processing programs used and the environmental temperature, volumes of gases
released per unit time are provided below for reference. Based on these values and the volumetric size
of the installation location, check if the ventilation rate is sufficient to meet the requirements at the
installation location pertaining to the concentrations of substances in the work environment. If no
ventilation system is available, use a 38 or 75 exhaust duct adapter (optional) to connect the duct
hose to the exhaust port of the instrument, and discharge the exhaust fume to the outside of the
building.
Maximum volumes of gases released per unit time (reference)
Ethanol: 3.9×10-3 (m3/hr)
Xylene: 4.8×10-4 (m3/hr)

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 Installation Method
Checking the Work Area
The dimensions and weight of the instrument in packed state are as follows.
Check them beforehand so that sufficient space and safety can be ensured when "transporting,
unpacking and installing" the instrument.

"Dimensions and weight in packed state" "Instrument dimensions and weight

W: Approx. 1500 mm W: Approx. 1250 mm (excluding the display)
H: Approx. 930 mm H: Approx. 630 mm
D: Approx. 900 mm D: Approx. 713 mm
Weight: Approx. 210kg Weight: Approx. 150kg

Transportation

When transporting the instrument on a pallet truck, etc., one person should not work alone in order to
prevent instrument damage or accident caused by rear-end collision, dropping of the instrument, etc.
When transporting the instrument, place thin iron sheets, etc., to eliminate height gaps on the floor so
that the instrument will not receive impact.
Pay due attention to safety. Particularly when transporting the instrument on a slope, remember that,
because the instrument is heavy, stopping the instrument will not be easy once it began accelerating.

 Transporting the instrument to

the work area
• Transporting on a forklift or
pallet truck
When transporting the instrument
Inlet Inlet Inlet Inlet
on a forklift or pallet truck, do so
when the instrument is still
packed, and insert the fork into
the packing pallet as shown in the
figure below. Refer to the
palletizing size in the figure on the
Front Face of the Instrument
left and check the dimensions of
Side Face
the forklift or pallet truck
beforehand.
 Moving to the installation location
1. Unpack the instrument by referring to the next section, "Unpacking Procedure - Unpacking the
system," and move the instrument to the installation location.
2. If the instrument can be transported on a pallet truck, etc., move the whole packing pallet to near
the installation location.
3. Remove the L brackets securing the instrument to the packing pallet.
4. Screw in the transport handles into the treaded parts on the left and right side faces at the bottom
of the instrument. Securely screw in the transport handles all the way.
5. Hold the transport handles to lift the instrument and move it to the installation location.

When using a pallet truck, etc., for transport, confirm that the instrument and packing pallet are
secured together with L brackets, because otherwise the instrument may shift and drop from the
packing pallet. When the instrument must be pushed while being transported on a pallet truck, etc., pay
attention to where on the instrument to be pushed, because the exterior panels may be deformed or the
acrylic cover may be damaged. (Do this by holding the transport handles at the base of the system.)
Pay due attention to safety, and particularly when transporting the instrument on a slope, remember
that, because the instrument is heavy, stopping the instrument will not be easy once it began
accelerating. When lifting the instrument to move, be sure to secure a line of movement or footing.

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 Installation Method
Unpacking Procedure
How to remove the instrument from the packing box that has been carried in, and remove the protective
materials, is explained. To perform each work safely and correctly, be sure to follow the procedure
specified herein.

Confirm before unpacking the instrument, that the packing materials have not been damaged due to
external forces applied during transport. Two or more persons should always work together to unpack
the instrument. Be careful not to damage/scratch the instrument when unpacking. When taking the
accessory box out of the instrument, assume a natural posture.

Unpacking the System

1. Cut the bands with a cutting knife, etc. and remove the cap.
Cap

Sleeve

Ester band

2. Remove the cushion materials put around the instrument, and take out the sleeves and polyethylene
bags.

3. Remove the cushion materials inserted into the front cover from both sides, lift the front cover, and take
out the cushion materials sandwiched between the front cover and the front door.

Cushion material Cushion material Cushion material

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 Installation Method
4. Remove the tape securing the top cover, and take out the cushion materials sandwiched between the
cover and the accessory box.

Tape
Cushion material

5. Cut the PP band and take out the accessory box.

Accessory box

PP band

6. Remove the cushion materials inserted underneath the display arm, and the cushion foams wrapping
the instrument.

Cushion material Cushion foam

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 Installation Method

Removing the Securing Members Inside the Instrument

1. Remove the cushion materials sandwiched between the robot arm and the rail.

Cushion material

2. Remove the tape securing the holder, and remove the cushion materials inserted underneath the
holder.
Tape Cushion material

3. Remove the reinforcing cushion materials on the tray, and take out the cushion foam sheet and PP
band.

Cushion material Cushion material Cushion material PP band Cushion foam sheet

4. Remove all 3-position and 4-position reservoir trays stored in the system, and remove the tape
securing the cushion foam sheet and the water supply nozzle.

Cushion foam sheet Sheet securing the water supply nozzle

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 Installation Method
Switching the Power-supply Voltage Setting
This instrument supports multiple voltages, but the setting on the instrument side must be switched
according to the power-supply voltage. If the instrument is used without switching the voltage
setting, not only the instrument will break down, but the user may also be exposed to danger;
accordingly, contact the Sakura Finetek Technical Support representative or local representative
when the instrument is installed to switch the voltage setting according to the voltage on the facility
side. The factory setting is 230 V.

Checking the Accessories

Product models in the instruction sheet are as shown below. Pay attention that accessories are different
depending on the model.
Model Overview
Tissue-Tek PrismaPlus (Model Prisma-P-AD) w/ Drying Function
Tissue-Tek PrismaPlus (Model Prisma-P-AS) w/ Drying/Heating/Special Staining Functions
Tissue-Tek PrismaPlus (Model Prisma-P-ED) w/ Drying Function
Tissue-Tek PrismaPlus (Model Prisma-P-ES) w/ Drying/Heating/Special Staining Functions
Tissue-Tek PrismaPlus (Model DRS-Prisma-P-JD) w/ Drying Function
Tissue-Tek PrismaPlus (Model DRS-Prisma-P-JS) w/ Drying/Heating/Special Staining Functions
Tissue-Tek PrismaPlus (Model DRS-Prisma-P-JCS) w/ Drying/Heating/Special Staining Functions
Confirm that all accessories (refer to the table below) are present and that the instrument and
accessories are free from damage, and if the instrument is damaged or any of the accessories is short
or damaged, contact the Sakura Finetek sales representative.
No. Name of part Q'ty Remarks
1 3-position reservoir tray (2-phase stainless) *3 3 -AD, -AS, -JD, -JS, -JCS
2 3-position reservoir tray 3 -ED. -ES
3 4-position reservoir tray (2-phase stainless) *3 3 -AD, -AS, -JD, -JS, -JCS
4 4-position reservoir tray 3 -ED. -ES
5 Control panel protection sheet 1 Comes attached to the control panel
6 Paraffin tray 2
7 Hot air outlet panel, drying station 2
8 Bushing (bolt cap) 4
9 Heating solution reservoir 2 -AS, -ES, -JS, -JCS
10 Water supply hose (faucet connection type) 1 -ED, -ES, -JD, -JS, -JCS
11 Nipple (1/2 inch) 1(2) 2 for -ED, -ES
12 Bushing (1/2 x 3/4 inch) 1 -ED, -ES, -JD, -JS, -JCS
13 Bushing (machined) 1 -AD, -AS
14 Drain hose unit (38 mm × 2 mm + cuffs) 1
15 Drain elbow 1
16 Spiral wire band 1
17 Hose clamp 1
18 Power cord 100/115 VAC power plug 1 Not applicable to -JCS
19 Power cord 230 VAC power plug 1
20 Ferrite core 1 For input in case of power outage
21 Activated carbon filter 2
22 20-slide basket 10
23 20-slide basket adapter 10
24 Nozzle 4 Of the four pieces, two are preinstalled on the instrument.
25 Wash cap 4 Of the four pieces, two are preinstalled on the instrument.
26 Cap for covering water supply port 4 Of the four pieces, two are preinstalled on the instrument.
27 Cap for LAN port 1 For LAN port; installed on the port.
28 Cap for RS232C port 1 For RS232C port; installed on the port.
29 CF card 1
30 Station labels (Start, End) 1 3 types/set
31 Basket adapter label 1
32 Operating Manual 1
33 Package insert for medical equipment 1 -JD, -JS
34 Excerpt from Unpacking/Installation Manual 1
35 Warranty registration card 1 -JD, -JS
36 10-slide basket 10 -AS, -ES, -JS, -JCS
37 10-slide basket adapter 10 -AS, -ES, -JS, -JCS
38 10-slide basket load adapter 3 -AS, -ES, -JS, -JCS
39 Special stain solution reservoir 8 -AS, -ES, -JS, -JCS
40 Lid, special stain solution reservoir 8 -AS, -ES, -JS, -JCS
41 Special stain reservoir tray (2-phase stainless) 1 -AS, -JS, -JCS
42 Special stain reservoir tray 1 -ES
43 Standard solution reservoir 22 -AD, -AS, -ED, -ES, -JCS
44 Solution reservoir with handle 6 -AD, -AS, -ED, -ES, -JCS
45 Wash reservoir 4 -AD, -AS, -ED, -ES, -JCS
46 Lid, standard solution reservoir 8 -AD, -AS, -ED, -ES, -JCS
47 Large lid, 3-position reservoir tray (2-phase stainless) 3 -AD, -AS, -JCS
48 Large lid, 3-position reservoir tray 3 -ED. -ES
49 Large lid, 4-position reservoir tray (2-phase stainless) 3 -AD, -AS, -JCS
50 Large lid, 4-position reservoir tray 3 -ED. -ES
Standard solution reservoir set (sold as a set) *2 1
Standard solution reservoir 22
51 Solution reservoir with handle 6 -JD, -JS
(Breakdown)
Wash reservoir 4
Lid, standard solution reservoir 28
Small solution reservoir set (sold as a set) *2 1
Solution reservoir with handle 6
Wash reservoir 4
52 Lid, standard solution reservoir 6 -JD, -JS
(Breakdown)
Small solution reservoir 44
Lid, small solution reservoir 44
20-slide basket load/unload adapter 3
53 Strainer 1 -ED. -ES
54 Street elbow (1/2 inch)

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 Installation Method
*1 The accessories are compatible with all models, unless the applicable models are specified in the Remarks field.
*2 Select either the standard solution reservoir set or small solution reservoir set. (-JD/-JS only)
*3 Housed in the instrument.

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 Installation Method
Checking the Installation (Securing the Instrument)
1. Confirm that the location where the instrument will be installed is flat and sufficiently strong.
2. Two or more persons work together to lift the instrument and move it to the installation location. If the
instrument rattles or is inclined too much when installed, adjust the level adjusters underneath the
instrument. Also when the instrument is linked to the Tissue-Tek Film or Tissue-Tek Glass g2, use the
level adjusters underneath the instrument to adjust the height and inclination of the instrument.
3. Once the instrument has been installed, remove the transport handles and install the attached bushing
(bolt caps) in the threaded parts on the system.

Installing the parts around the instrument

● Attach the water supply hose connect it to a water supply facility (tap water supply) meeting the
following conditions:
Pressure: Dynamic pressure = 0.098 to 0.441 MPa
Maximum static pressure = 0.74 MPa
Temperature: 30C max. (non-freezing)
Faucet: A faucet of general shape having a tip of 12 mm to 17 mm in size. (The
water supply hose cannot be connected to a faucet of any other size or to a
special shaped faucet such as chemical faucet or aerator faucet.)
Facility requirements: Use a facility meeting the following items in order to prevent health damage
caused by flow-back:
[1] Water supply facility not directly connected to public water pipes
[2] Water supply facility not directly connected to a water supply system
used for drinking water, etc.
[3] Water supply facility designed to prevent flow-back caused by reverse-
siphoning
• Water supply facility equipped with a flow-back protection mechanism
meeting the law of each country/region

1. Connect a nipple o the water supply port on the instrument via a bushing, and then screw the threaded
part of the water supply hose into the bushing.
2. Pull the flange on the other end of the water supply hose toward the hose and separate the water tap
joint.
3. Loosen the four screws on the water tap joint.
4. Tighten the four screws uniformly while pressing the black rubber in the water tap joint against the tip
of the water faucet.
5. Turn and tighten the nipple.
6. Insert the water supply hose into the water tap joint with the flange pulled back.
7. Gently pull the water supply hose by hand to confirm that it does not come off from the water tap joint.

Threaded part of the water hose

Water supply port Nipple Bushing

]
[1]

[2]

How to separate the water tap joint Water tap joint Nipple Flange on the water
supply hose

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 Installation Method
● Attaching the water drain hose
Facility requirements: [1] Sink, drain ditch or other water drain facility not subject to back pressure
[2] Water drain facility that will not negatively affect the environment or
human body even when chemicals used for staining enter the system
1. Install a spiral wire band on one end of the water drain hose, connect a drain elbow to the drain hose,
and then tighten the screws.
2. Install a hose clamp to the drain port on the instrument, and then insert the drain elbow into the drain
port.
3. Tighten the screws on the hose clamp using a flathead screwdriver.

Spiral wire band Drain port

Drain hose
Drain hose
Hose clamp
Drain elbow
4. Connect the other end of the drain hose to a sink having a minimum drain capacity of 15 L/min.
(Connect the hose securely so that it will not separate from the sink.)
5. Ensure that the drain port on the instrument is positioned higher than the sink, that the drain hose has
no slack in the middle, and that the outlet of the drain hose is away from the water level of the sink by
at least 100 mm.

Do not extend the drain hose using a different hose. The drain elbow can be oriented to either right or
left.
The drain hose contains vinyl chloride, so be careful not to spill organic solvents onto the hose.
After connecting the water supply hose and drain hose, confirm by referring to P. 1-42 that water is not
leaking.

● Installation of exhaust system

The instrument generates volatile solution gases, so install it where a ventilation system, local exhaust
system or other exhaust facility, or exhaust port leading to the outside of the building, is available. Also,
estimate the exhaust gas concentration from the reference value of maximum volume of gas released
and the volumetric size of the installation location, and confirm that the ventilation rate of the installed
ventilation facility is sufficient to meet the requirements pertaining to the concentrations of substances
in the work environment. Ideally the instrument is installed directly near a ventilation facility, if it must
be installed away from a ventilation facility; however, use a 38 or 75 exhaust duct adapter (optional)
to connect the duct hose to the exhaust port of the instrument, and discharge the exhaust fume near
the exhaust facility. Even when no exhaust facility is available, use a duct hose to discharge the
exhaust fume to the outside of the building.

Maximum volumes of gases released per unit time (reference value)

• Ethanol: 3.9×10-3 (m3/hr)
• Xylene: 4.8×10-4 (m3/hr)

 Facility Check  Required Options

• Ventilation system • Exhaust duct adapter, 38 mm dia.
• Local exhaust system • Exhaust duct adapter, 75 mm dia.
• Exhaust port

 How to Connect
1. Attach the 38 or 75 exhaust duct adapter to the exhaust port located at the back of the system on
the left side.
2. Insert a duct hose into the adapter and tighten it with a wire clamp, etc.
3. Bring the open end of the duct hose to near a ventilation system or local exhaust system or to the
outside of the building.
The duct hose should not be longer than 10 m, and confirm that the hose is not bent or crushed in the
middle. If the ambient temperature is low, drain water may collect inside the duct hose; accordingly,
make sure drain water will not collect in any part of the duct hose and also install a drain pan at the tip
of the duct hose or take other appropriate measure.

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 Installation Method
● Installing the water supply strainer (Prisma-P-ED, ES Only)
Depending on the condition of the maintenance space available on the right side of the instrument,
install the attached water supply strainer on the water supply port, and then connect the water supply
hose.

1. Enough maintenance space of 200 mm or more is available on the right side face of the instrument.
(1) Screw the connection nipple and bushing into the attached strainer.
Bushing
Nipple
Strainer Strainer type

(2) Screw the attached nipple, and the strainer set assembled in (1), into the water supply port on the
right side face of the instrument.

Nipple
Strainer type

(3) Screw in the water supply hose, and connect the water supply hose to the tap water supply
according to P. 1-9.

Water supply hose

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 Installation Method
2. No more than 100 to 200 mm of maintenance space is available on the right side face of the
instrument
In this case, approx. 35 mm of space may be required at the back of the instrument.
(1) Screw the connection nipple and bushing into the attached strainer.
Bushing
Nipple
Strainer Strainer type

(2) Screw the attached nipple and street elbow into the water supply port on the right side face of the
instrument.

Nipple
Street elbow

(3) Screw in the strainer set assembled in (1).

Strainer type

(4) Screw in the water supply hose, and connect the water supply hose to the tap water supply
according to P. 1-9.

Water supply hose

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 Installation Method
● Setting the water supply nozzle
The wash stations are set in the 4-position reservoir tray at the rearmost position of the cabinet. When
the 4-position reservoir tray is removed, you will see four water supply nozzles. If you have changed the
positions or quantity of wash stations, change the nozzle positions accordingly. The water supply nozzle
can be replaced as follows:

1. Remove the 4-position reservoir tray at the installed position of the wash reservoir.
2. Remove the cap or wash station nozzle currently set.
The cap can be removed by turning it counterclockwise. To remove the nozzle, turn the wash cap
counterclockwise to remove it, and then remove the wash station nozzle by pulling it straight up.
3. Set the cap if you want to seal the water supply part. At this time, confirm that a waterproof rubber
gasket is placed inside the hole, and then turn the cap clockwise to secure it.
4. To install the wash station nozzle, first align the small hole in the waterproof rubber gasket with the
small hole in the water supply outlet. Next, put the small projection on the bottom face of the wash
station nozzle into the small hole in the water supply outlet. Lastly, put the wash cap on and turn it
clockwise to secure the cap.

Wash station nozzle Cap

Wash port

Rubber Wash station Wash cap

gasket nozzle

Rubber
gasket

Cap

Wash
station
nozzle

Wash cap

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 Installation Method
Installation of Accessories

● Installing the activated carbon filter

1. Remove the tape securing the fume control unit door, and open the door [1].
2. Set the attached activated carbon filters, and close the door.

[1]

Fume control unit door Activated carbon filter (2 pcs)

● Installing the arm holder (attachment for standard solution reservoir)

1. Align the grooves on both sides of the tab on the arm with the grooves provided on the inside of the
arm holder, and install the arm holder from above.
2. The arm holder has been securely installed when the lock makes a "click" sound.
3. To remove the arm holder, raise the arm holder [2] while pulling the lock toward you [1].

The holding part of the basket adapter is sharp, so exercise caution.

Lock
[1]

[2]
Arm holder

Set the arm holder

by aligning the
grooves on the
inside with the
grooves provided
on the arm side.

Arm grooves

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 Installation Method
● Installing each tray
Set each tray with the two holes provided on the outer side of the handles on both sides of the tray
(see the figure below) facing the rear. The position of each tray is shown below. Trays cannot be placed
in the positions filled with gray.

3-position reservoir tray (for standard/small solution reservoirs)

Hole Hole
Back

Near side

4-position reservoir tray (for standard/small solution reservoirs)

Hole Hole
Back

Near side

Special stain reservoir tray (for special stain solution reservoirs)

Back Hole Hole

Near side

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 Installation Method
● Installing the solution reservoirs
Set the solution reservoirs in each tray. There are three types of solution reservoirs: standard solution
reservoir, small solution reservoir and special stain solution reservoir. The type of applicable tray and
setting orientation are different for each solution reservoir type.

• Type
A 3-position or 4-position reservoir tray contains standard solution reservoirs and small solution
reservoirs. A special stain reservoir tray contains only special stain solution reservoirs.
• Direction
Orient each solution reservoir so that the "solution name tab" on top of the reservoir faces the front side
of the instrument, and set the reservoir by aligning the "grooves" on both sides of the reservoir with the
grooves in the tray. Pay attention to the widths of the grooves provided on both sides of the solution
reservoir. When the solution reservoir is set in the tray, the wider grooves should come to the left, while
the narrower grooves should come to the right.

Back
Left tray grooves Right tray grooves
Wider than those * Narrower than
on the right those on the left

Near side
3-position 4-position Special stain
reservoir tray reservoir tray reservoir tray

Left grooves
Wider than those
on the right Right grooves
* Narrower than
those on the left

Solution name Standard solution Small solution Special stain

tab reservoir reservoir solution reservoir

Turning on the Power

Use a power cord appropriate for the power-supply voltage. Be sure to connect the power cord to a
power outlet with grounding bar.

1. Confirm that input voltage has been set according to the installation environment.
2. Connect the power cord to the power-supply inlet on the right side face of the instrument at the bottom.
3. Confirm that the power switch at the bottom right on the front face of the instrument is turned off, and
then connect the plug on the power cord to the power outlet on the facility side.
4. Turn on the power switch to start the instrument.

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 Specification "Prisma-P-AD/AS"
General name Automated slide stainer
Brand name Tissue-Tek Prisma®Plus
Type Prisma-P-AD Prisma-P-AS
Product code 6170 6171
Manufacturing license number 20B2X00014000034
1250 (W) x 713 (D) x 650 (H) mm (excluding the control
Dimensions
monitor)
Weight Approximately 150 kg
Rated power supply Single-phase 115 VAC ± 10%, 50/60 Hz, 11 Amps
Power consumption 1000 VA
Electric shock protection Class I, overvoltage category II
Pollution degree Pollution degree I
Batch mode: 60, 20 or 10 slides
Stain process
Continuous mode: Up to 11 runs can be set in the system.
Up to 50 programs can be stored.
Staining programs
Capabilities One program can consist of up to 50 steps.
Standard solution name Up to 100 solution names can be stored.
Solution configuration
Up to 50 solution configurations can be stored.
name
Drying station Temperature setting range: 30 to 65C
Temperature setting range: 30 to
Heating station -
70C
Voltage = Rated voltage  10%, 50/60 Hz
Current capacity = Rated current min.
Power supply
Grounding = Comes with grounding bar conforming to
class D grounding
Water = Tap water
Pressure
Required Dynamic water = 0.098 to 0.441 MPa
facilities Water supply
Maximum static pressure = 0.74 MPa
Temperature = 30C max. (non-freezing)
Nominal pipe diameter = 15A (1/2 in) min.
Method = Indirect draining
Drain Drain capacity = 15 L per minute min.
Nominal pipe diameter = 40A (1-1/2 in) min.
Operating Ambient temperature 10 to 40C
environment Relative humidity 30 to 85% (non-condensing)
Storage Ambient temperature -10 to 65C (instrument interior must be drained.)
environment Relative humidity 20 to 90% (non-condensing)
Drop test JISZ0200:2013 (Level 1)
Vibration/drop Vibration test JISZ0232:2004 (Standard of Truck
Transportation)
Noise 65 dB or less (A characteristics)
Law FDA Class 1
UL 61010-1:2012
CAN/CSA-C22.2 No. 61010-1-12: 2012
Electrical safety evaluation
CAN/CSA-C22.2 No. 61010-2-010-04: 2014
CAN/CSA-C22.2 No. 61010-2-101-04: 2014
Law and
conforming Risk management ISO 14971: 2007
standard IEC 62366: 2007
Medical equipment
IEC 62304: 2006
software
Quality management ISO 13485: 2003
Electromagnetic
IEC61326-2-6:2005
compatibility

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 Specification "Prisma-P-ED/ES"

General name Automated slide stainer

Brand name Tissue-Tek Prisma®Plus
Model Prisma-P-ED Prisma-P-ES
Product code 6172 6173
Manufacturing license number 20B2X00014000034
Dimensions 1250 (W) x 713 (D) x 650 (H) mm (excluding the control monitor)
Weight Approximately 150 kg
Rated power supply Single-phase 230 VAC ± 10%, 50/60 Hz, 5.5 Amps
Power consumption 1000 VA
Electric shock protection Class I, overvoltage category II
Pollution degree Pollution degree I
Batch mode: 60, 20 or 10 slides
Stain process
Continuous mode: Up to 11 runs can be set in the instrument.
Up to 50 programs can be stored.
Capabilities Staining programs
One program can consist of up to 50 steps.
Standard solution name Up to 100 solution names can be stored.
Solution configuration name Up to 50 solution configurations can be stored.
Drying station Temperature setting range: 30 to 65C
Heating station - Temperature setting range: 30 to 70C
Voltage = Rated voltage  10%, 50/60 Hz
Power supply Current capacity = Rated current min.
Grounding = Comes with grounding bar conforming to class D
grounding
Water = Tap water
Pressure
Required Dynamic water = 0.098 to 0.441 MPa
facilities Water supply
Maximum static pressure = 0.74 MPa
Temperature = 30C max. (non-freezing)
Nominal pipe diameter = 15A (1/2 in) min.
Method = Indirect draining
Drain Drain capacity = 15 L per minute min.
Nominal pipe diameter = 40A (1-1/2 in) min.
Operating Ambient temperature 10 to 40C
environment Relative humidity 30 to 85% (non-condensing)
Storage Ambient temperature -10 to 65C (instrument interior must be drained.)
environment Relative humidity 20 to 90% (non-condensing)
Drop test JISZ0200:2013 (Level 1)
Vibration/drop
Vibration test JISZ0232: 2004 (Standard of Truck Transportation)
Noise 65 dB or less (A characteristics)
CE Marking
Law IVD Directive (98/79/EC)
RoHS Directive (2011/65/EU)
CE Marking & IVD Directive
EN 61010-1: 2010
Law and Electrical safety evaluation
EN 61010-2-010: 2003
conforming
standard EN 61010-2-101: 2002
Risk management EN 14971: 2012
EN 62366: 2008
Medical equipment software EN 62304: 2006
Quality management ISO 13485: 2004
Electromagnetic compatibility EN 61326-2-6: 2006

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 Specification "DRS-Prisma-P-JD/JS"
"DRS-Prisma-P-JCS"
General name Automated slide stainer
Brand name Tissue-Tek Prisma®Plus
Model Prisma-P-JD Prisma-P-JS Prisma-P-JCS
Product code 6174 6175 6177
Manufacturing license number 20B2X00014000034
Dimensions 1250 (W) x 713 (D) x 650 (H) mm (excluding the control monitor)
Weight Approximately 150 kg
Rated power supply Single-phase 100 VAC ± 10%, 50/60 Hz, 10 Amps
Single-phase 230 VAC ± 10%, 50/60 Hz, 5.5 Amps
Power consumption 1000 VA
Electric shock protection Class I, overvoltage category II
Pollution degree Pollution degree I
Stain process Batch mode: 60, 20 or 10 slides Continuous mode: Up to 11 runs can be set
in the instrument .
Staining programs Up to 50 programs can be stored. One program can consist of up to 50
Capabilities
steps.
Standard solution name Up to 100 solution names can be stored.
Solution configuration name Up to 50 solution configurations can be stored.
Drying station Temperature setting range: 30 to 65C
Heating station - Temperature setting range: 30 to 70C
Power supply Voltage = Rated voltage  10%, 50/60 Hz
Current capacity = Rated current min.
Grounding = Comes with grounding bar conforming to class D grounding
Water supply Water = Tap water
Pressure
Required Dynamic water = 0.098 to 0.441 MPa
facilities Maximum static pressure = 0.74 MPa
Temperature = 30C max. (non-freezing)
Nominal pipe diameter = 15A (1/2 in) min.
Drain Method = Indirect draining
Drain capacity = 15 L per minute min.
Nominal pipe diameter = 40A (1-1/2 in) min.
Operating Ambient temperature 10 to 40C
environment Relative humidity 30 to 85% (non-condensing)
Storage Ambient temperature -10 to 65C (instrument interior must be drained.)
environment Relative humidity 20 to 90% (non-condensing)
Drop testJISZ0200:2013 (Level 1)
Vibration/drop
Vibration test JIS Z0232: 2004 (Standard of Truck Transportation)
Noise 65 dB or less (A characteristics)
Applicable instruments DRS-Prisma-P-JD/JS DRS-Prisma-P-JCS
Pharmaceutical and Medical Device Act
(Manufacturing and Sales Notification, Export
Law Regarding Ensuring of Notification), CFDA, Ordinance on
Quality, Effectiveness and Supervision and Control of Medical Devices,
Law
Safety of Drugs, Medical Class 1 Equipment, Administrative Regulation
Equipment, Etc., and Other Pertaining to Limitations of Use of Harmful
Substances in Electrical and Electronic
Products
Law Regarding Ensuring of
Law and Quality, Effectiveness and CFDA, Ordinance on Supervision and Control
conforming Safety of Drugs, Medical of Medical Devices, Class 1 Equipment
standard Electrical safety evaluation Equipment, Etc., and Other
GB 4793.1-2007 (IEC 61010-1: 2010)
JISC 1010-1: 2014 GB 4793.6-2006 (IEC 61010-2-010: 2003)
JISC 1010-2-101: 2013 YY 0648-2008 (IEC 61010-2-101: 2002)
Risk management JIST 14971: 2012
Medical equipment software JIST 2304: 2012
Quality management ISO 13485: 2005
Electromagnetic GB/T 18268.1-2010 (IEC 61326-1: 2005)
JISC 1806-2-6: 2012
compatibility GB/T 18268.26-2010 (IEC 61326-2-6: 2005)

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 Name of Each Part

[1] [2] [3] [4] [5]

[10]
[6]

[7] [11]

[8] [9]

[1] Cover
The cover protects the operator against exposure to dangerous chemicals without
obstructing his/her view.
[2] Door
Open this door when setting or removing tissues.
[3] Water supply strainer door
Access this area when maintaining the water supply circuit.
[4] Power switch
[5] Control panel display
A touch-panel type display for operating the instrument.
[6] External interface
It can be connected to an UPS (uninterruptible power supply), 2D bar code reader,
etc.
[7] Power-supply inlet
[8] Water supply port
Tap water is supplied to this port.
[9] Drain port
[10] Exhaust outlet
This outlet can be connected to an external exhaust duct via a special attachment.
[11] Automated coverslipper link port
Remove the port cover to connect an automated coverslipper for automated operation
encompassing staining through coverslipping.

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 Name of Each Part

[17] [18] [19]

[12] [13] [14] [15] [16]

[12] Solution reservoir

[13] Wash station
Up to 4 wash stations.
[14] Drying station
[15] Heating station
Heating stations permit special stain runs that require heating.
[16] Water supply handle
Use this handle to adjust the water flow rate.
[17] Fume control unit
Activated carbon filters are part of the unit.
[18] Robotic arm
The function of robotic arms is to move slide basket.
[19] CF card slot
This sets a CF card in slot.

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 Standard Accessories/Options
[1]
[5]

[2] [7]
[6] [8] [9]
[3]

[4]

[10]

[11]
[12]

[13] [16] [18]

[14]
[15] [21]
[17]

[19]

[23] [20]
[24]
[25]
[26] [22]
[27]
[29]
[28] [30]
[31]

[33]

[32]
[42]

[34]
[35]
[36] [38]
[39]

[102]
[101]

[37]

[40]

[104]

[41] [103]

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 Standard Accessories/Options
AD AS ED ES JD JS JCS
No. Product/part name Product code Product code 6170 6171 6172 6173 6174 6175 6177
Q'ty
1 10-slide basket adapter 6138 K24-418-01 - 10 - 10 - 10 10
2 10-slide basket 6137 K24-411-00 - 10 - 10 - 10 10
3 20-slide basket adapter 6136 K24-408-01 10 10 10 10 10 10 10
4 20-slide basket 4768 K16-500-08 10 10 10 10 10 10 10
5 Wash reservoir 6149 K24-403-01 4 4 4 4 - - 4
6 Solution reservoir with handle 6148 K24-400-00 6 6 6 6 - - 6
(1) (1)
7 3-position reservoir tray 6152 F6-02-7300 - - 3 3 - - -
(1) (1)
8 4-position reservoir tray 6154 F6-02-7400 - - 3 3 - - -
9 Special stain reservoir tray 6156 K24-147-02 - - - 1 - - -
10 Standard solution reservoir 6147 K24-401-01 22 22 22 22 - - 22
11 Small solution reservoir - K24-402-01 - - - - - - -
12 Special stain solution reservoir 6140 K24-404-00 - 8 - 8 - 8 9
13 Lid, standard solution reservoir 6151 K24-405-00 8 8 8 8 - - 8
14 Lid, small solution reservoir 6146 K24-406-00 - - - - - - -
15 Lid, special stain reservoir 6144 K24-407-00 - 8 - 8 - 8 8
16 10-slide basket load/unload adapter 6139 K24-488-03 - 3 - 3 - 3 3
17 20-slide basket load adapter 6135 K24-491-01 - - - - - - -
18 Heating solution reservoir 6141 K24-503-01 - 2 - 2 - 2 2
19 Paraffin tray - K24-531-04 2 2 2 2 2 2 2
20 Hot air outlet panel, drying station - K24-535-00 2 2 2 2 2 2 2
21 Station labels (Start, End) 6161 F6-02-8400 1 1 1 1 1 1 1
22 Basket adapter label 6162 K24-712-00 1 1 1 1 1 1 1
(3) (3) (3) (3) (3) (3)
23 Nozzle - K24-430-01 4 4 4 4 4 4 4(3)
(3) (3) (3) (3) (3) (3)
24 Wash cap - K24-433-00 4 4 4 4 4 4 4(3)
25 Cap for covering water supply port - K24-437-00 4(3) 4(3) 4(3) 4(3) 4(3) 4(3) 4(3)
26 Nipple (1/2 inch) - A6-30-0040 1 1 2 2 1 1 2
27 Bushing (1/2 x 3/4 inch) - A6-34-5160 - - 1 1 1 1 1
28 Water supply hose (faucet connection type) - B7-44-0030 - - 1 1 1 1 1
29 Drain elbow - K23-291-00 1 1 1 1 1 1 1
30 Hose clamp - D9-00-0159 1 1 1 1 1 1 1
31 Spiral wire band - D9-00-0146 1 1 1 1 1 1 1
32 Drain hose unit (38 mm x 2 m + cuffs) - K24-094-00 1 1 1 1 1 1 1
33 Activated carbon filters 6160 K24-467-00 2 2 2 2 2 2 2
34 Ferrite core - A3-33-6034 1 1 1 1 1 1 1
35 CF card 6164 K24-718-00 1 1 1 1 1 1 1
36 Bushing (bolt cap) - A4-07-0511 4 4 4 4 4 4 4
(2) (2) (2) (2) (2) (2)
37 Control panel protection sheet 6163 K24-640-00 1 1 1 1 1 1 1(2)
38 Large lid, 3-position reservoir tray 6153 K24-423-03 - - 3 3 - - -
39 Large lid, 4-position reservoir tray 6155 K24-424-03 - - 3 3 - - -
40 Power cord - 00000-0507 1 1 1 1 1 1 -
41 Power cord - A4-01-0534 1 1 1 1 1 1 1
42 Strainer - 00000-3006 - - 1 1 - - -
- Bushing (machined) - K23-440-01 1 1 - - - - -
- Street elbow (1/2 inch) - A6-31-3000 - - 1 1 - - -
- Cap for LAN port - 00000-0910 1(5) 1(5) 1(5) 1(5) 1(5) 1(5) 1(5)
- Cap for RS232C port - 00000-0511 1(5) 1(5) 1(5) 1(5) 1(5) 1(5) 1(5)
- 3-position reservoir tray (2-phase stainless) 6180 A-MK25-U013-00-A 3(1) 3(1) 3(1) 3(1) 3(1)
- 4-position reservoir tray (2-phase stainless) 6182 A-MK25-U014-00-A 3(1) 3(1) 3(1) 3(1) 3(1)
- Special stain reservoir tray (2-phase stainless) 6185 A-MK25-U020-00-A 1 1 1
- Large lid, 3-position reservoir tray (2-phase stainless) 6181 A-MK25-0620-01-A 3 3 3
- Large lid, 4-position reservoir tray (2-phase stainless) 6183 A-MK25-0622-01-A 3 3 3
- Installation Manual - - 1 1 1 1 1 1 1
- Operating Manual - - 1 1 1 1 1 1 1
- Package insert - - - - - - 1 1 -
- Warranty registration card - - - - - - 1 1 -
No. Optional or set Product code Product code AD AS ED ES JD JS JCS
38 Large lid, 3-position reservoir tray 6153 K24-423-03
39 Large lid, 4-position reservoir tray 6155 K24-424-03
101 Exhaust duct adapter, 38 mm dia. - K24-478-01
102 Exhaust duct adapter, 75 mm dia. - K24-567-01
103 Tissu-Tek Film link system - F6-02-8700
104 2D bar code reader - 1900GRS-1RS
- Large lid, 3-position reservoir tray (2-phase stainless) 6180 A-MK25-0620-01-A
- Large lid, 4-position reservoir tray (2-phase stainless) 6182 A-MK25-0622-01-A
- Tissu-Tek Glas-g2 link kit - K0000-1189
- Standard solution reservoir set 6157 F6-02-7500 1(4) 1(4)
- Small solution reservoir set 6158 F6-02-7600 1(4) 1(4)
- Special stain solution reservoir set 6159 F6-02-7700
- Special stain solution reservoir set (2-phase stainless) 6184 A-MK25-U015-00-A
* (1) Preinstalled inside the instrument.
(2) Pre-attached on the control panel screen.
(3) Of the four pieces, two are preinstalled on the instrument.
(4) Select either the standard solution reservoir set or small solution reservoir set.
(5) Pre-installed on each port.

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 Basics
Basics on This Instrument
This section explains the Basics of operating the instrument. For the details of each item, refer to the page
indicated in the text.

Instrument Overview
● This instrument automatically stains tissues and cell smear samples on glass slides for microscopy, as
part of histological studies and tests conducted in the fields of pathology, anatomy, clinical pathology,
etc.
The instrument supports various types of staining such as H&E, Papanicolaou etc.
● This instrument is operated from the touch-panel type display located on the right side. This display
allows the operator to easily operate the instrument according to visual cues by touching the necessary
keys displayed on the screen. Staining data can also be output from a PC by using a special compact
flash card (CF card).
● Two types of baskets can be used for storing slides: the 20-slide type for general staining and the 10-
slide type for special staining
● Solution reservoirs are largely classified into three types: standard solution reservoir, small solution
reservoir and special stain solution reservoir. The basket capacity and required solution volume of each
solution reservoir are summarized below.

Standard solution
Small solution reservoir Special stain reservoir
reservoir
20-slide basket 3 baskets 1 basket -
10-slide basket 3 baskets 1 basket 1 basket
Required solution volume 680 ~ 820 mL 255 ~ 285 mL 160 ~ 180 mL

Stain Process Modes

This instrument performs stain process in the "continuous mode" and "batch mode." The two staining
modes are explained below.

• Continuous mode
A different staining run can be started during the current staining run, with up to 11 staining runs
performed simultaneously.
• Batch mode
In this mode, each stain process must be completed before the next sample can be stained. The start
step and end step can be specified to start a staining run.

Refer to P. 1-33 on how to set each mode.

User Management by Password

The instrument can limit the operating functions permitted to each user according to the user’s password.
Two types of passwords are provided. The "Administrator Password" is a higher-level password that
permits all operations, while the "User Password" is a lower-level password that permits only the specific
operations pre-defined for each user. Refer to P. 1-39 for details on how to set a password, etc.

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 Basics
Logon/Logoff Function
The user enters his/her pre-assigned password to log on to the instrument, upon which the instrument
becomes operational. Only the user who has logged on can operate the instrument. The operations that
are permitted to the logged-on user vary depending on the level of the user’s password.
When the user logs off from the instrument during operation, unauthorized instrument operation by a third
person can be prevented. The stain process is not affected by the logoff, and will continue until the end. To
log on to the instrument again, the user enters a password using the Log On key in the monitor screen.
The logon password need not be the same as the one that was used to log off from the instrument. The
user can log on using any password currently stored in the instrument.
During any operation other than stain process, the user can also freely log off from the instrument when
leaving from the instrument for an extended period of time. This function protects the instrument against
unauthorized operation by a third person.

Safety Mechanism of the Instrument

As a safety mechanism, the instrument is designed so that the arm will stop moving if the cover is opened
during stain process. Although the staining time counter keeps counting while the cover is open, the
baskets do not move because the arm is stopped. Staining can be resumed by performing the resumption
operation after closing the cover. However, the scheduled completion of stain process may be delayed by
up to the period during which the cover has been open. The arm will also stop if the front door is opened
when a solution station in the frontmost row is being accessed. In this case, stain process can also be
resumed by performing the resumption operation after closing the front door. However, the scheduled
completion of staining process may be delayed by up to the period during which the door has been open.

How to Open and Close the Cover/Door

● How to open the cover
Hold the stainless part at the center of the cover, and raise the cover while pulling it toward you. As the
cover rises, it automatically tilts forward. Push the cover into the back of the instrument, and the cover
will be stored into the ceiling of the instrument.
● How to close the cover
Hold the stainless part at the center of the cover and pull it toward you. Once the cover has been fully
drawn out of the ceiling of the instrument, the stainless part starts tilting down. When the cover drops
to the bottom, push the stainless part into the instrument to securely close the cover.
● How to open the door
Touch the center of the door once, and the door will open toward you.
● How to close the door
Raise the center of the door and push in the door until a click sound is heard in the reverse order of
door opening.

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 Basics

Handling the Touch-panel Display

The touch-panel display used for operating the instrument is movable. To move the display, do so slowly
by holding both ends of the display. Note that the moving range of the display is limited. Do not move the
display beyond the specified range or apply any excessive force, as it may cause the display to break.
A protection sheet is attached on the control panel. A protection sheet that has become dirty, damaged or
peeled must be replaced.

Handling the CF Card Slot

A CF card slot for setting a CF card is provided on the left side of the touch-panel display used for
operating this instrument. You can save staining and other data stored in the instrument to a PC, etc.,
using a CF card.

• Set a CF card into the CF card slot from the side with an arrow, with the other side on which a label is
attached facing toward you.
• Be sure to wait for the confirmation message to appear on the screen before removing the CF card.
• Sakura does not warrant the integrity of data stored in CF cards regardless of the failure exhibited by the
instrument or monetary damage suffered in connection with the data loss/damage. Sakura does not
provide any service associated with the recovery or restoration of data in CF cards.
• CF cards other than those specified for the instrument may not be supported. CF cards must be FTP-
formatted using Microsoft Windows.

CF card slot

Arrow Label

CF card

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 Basics
Various Gateways
External signal I/O connections for
various gateways are provided on the
right side of the instrument. You can
connect the instrument to an
LAN connection External alarm
uninterruptible power supply, alarm
output
output, LAN, and bar code reader from
these gateways. Each gateway is External alarm output
explained below. Power-outage detection signal input

Bar code reader connection

● Power-outage detection signal

You can connect an uninterruptible power supply (UPS) to the instrument’s power supply so that
tissues can be preserved and evacuated in case of power outage. When installing an UPS, be sure to
use a model with an output capacity equivalent to or greater than the input rating of the instrument.

Recommended UPS rating: Capable of outputting 15 kVA for 5 minutes

Some UPSs are not supported by the instrument. For details, contact the Sakura Finetek Technical
Support representative or local representative. The instrument provides an UPS input signal to detect a
power outage mode of the UPS connected to the power supply. Use an UPS equipped with a contact-
output interface or open-collector output that remains open in normal state and shorts upon power
outage. Always turn off the instrument power before connecting an UPS.

* When connecting the power-outage detection signal to an external circuit, loop the signal cable
around the attached ferrite core at a point as close as possible to the instrument, as shown below. If
the ferrite core is not installed, the instrument may malfunction due to external noise.

To the instrument’s power-outage detection signal input To the UPS’s power-outage detection signal output

SEIWA

When the ferrite core is installed

Example of power-outage signal connection

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 Basics
● Alarm output
This is a no-voltage contact output used to retrieve an alarm from the instrument in case of an error
that stops the staining operation (error other than battery low, exhaust fan and heating/drying station
errors). The rated voltage of the connected load must not exceed 30 VDC, with the rated current kept
to 1 A or below. Always turn off the instrument power before connecting the alarm output. For details,
contact the Sakura Finetek Technical Support representative or local representative.

Example of external alarm connection

● EMC
This instrument conforms to the emission and immunity requirements specified in EN61326-2-6 or
JISC1806-2-6.

● Ethernet port (LAN connection)

Special settings are needed to connect the instrument to a local area network. Should you require LAN
connection, consult your system administrator. For details, contact the Sakura Finetek Technical
Support representative or local representative.

Specification: 10BASE-T

● 2D bar code reader (optional)

By connecting the 2D bar code reader, the instrument can read the bar codes on Sakura staining
solutions*. Their expiration dates can be entered in the system based on the bar code information.

* Check the local Sakura Finetek website for availability of the Sakura staining solutions and staining
kits.

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 Basics
Relationship of Solution Configurations and Station Numbers
This section explains the relationship of solution configurations and station numbers shown on the screen.
The link station is available only when the instrument is connected to an automated coverslipper. It cannot
be used if an automated coverslipper is not connected.

● Arrangement of small solution stations

The small solution stations can be arranged "without special solution stations" or "with special solution
stations." In both cases, the solution configurations and number of stations are the same. However, the
number of stations and station numbers vary depending on whether some stations are used as wash
stations or solution stations.

Station 1 Station 3 Station 5 Station 13

Row 4 Station 7 Station 8 Station 9 Station 10
Station 2 Station 4 Station 6 Station 14
Station 15 Station 17 Station 19 Station 21 Station 23 Station 25 Station 27 Station 29
Row 3
Station 16 Station 18 Station 20 Station 22 Station 24 Station 26 Station 28 Station 30
Station 31 Station 33 Station 35 Station 37 Station 39 Station 41 Station 43 Station 45
Row 2
Station 32 Station 34 Station 36 Station 38 Station 40 Station 42 Station 44 Station 46
Frontmost row Station 53 Station 47 Station 48 Station 49 Station 50 Station 51 Station 52
Figure Arrangement with/without Special Solution Stations

Fixed station numbers are assigned to the start stations (S1 to S3), end stations (linked) (E1-1 to E1-5),
end stations (not linked) (E2-1 to E2-5), wash stations (W1 to W4), drying stations (D1A, D1B, D2A, D2B),
heating stations (H1, H2) and link station (LINK). The tables below specify the identification number of
each station attribute and the corresponding station number.

Start station S1 S2 S3
Without special solution stations
Station 52 Station 51 Station 50
With special solution stations
* S2 and S3 can also be set as solution stations.

End station (linked) E1-1 E1-2 E1-3 E1-4 E1-5 PE station

Without special solution stations The rightmost end station is
Station 47 Station 48 Station 49 Station 50 Station 51 selected as the PE station
With special solution stations
* E1-2 to E1-5 can also be set as solution stations.

End station (not linked) E2-1 E2-2 E2-3 E2-4 E2-5

Without special solution stations
Station 47 Station 48 Station 49 Station 50 Station 51
With special solution stations
* E2-2 to E2-5 can also be set as solution stations.

Link station LINK Wash station W1 W2 W3 W4

Without special solution stations Without special solution stations
Station 53 Station 4 Station 5 Station 6 Station 7
With special solution stations With special solution stations
* The link station can be set only when the instrument * W1 to W4 can also be set as solution stations.
is connected to an automated coverslipper.

Drying station D1A D1B D2A D2B Heating station H1 H2

Without special solution stations Without special solution stations
Station 13 Station 14 Station 29 Station 30 Station 45 Station 46
With special solution stations With special solution stations
* Available with the DRS-Prisma-J0S only.

Special solution station SP1 SP2 SP3 SP4 SP5 SP6 SP7 SP8
Without special solution stations − - - - - - - -
With special solution stations Station 21 Station 22 Station 23 Station 24 Station 25 Station 26 Station 27 Station 28

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 Basics
● Arrangement of standard solution stations (with drying stations)
In the standard mode, the arrangement and number of stations vary depending on whether the solution
stations are arranged "without special solution stations" or "with special solution stations."

Station 1 Station 2 Station 3 Station 4 Station 5 Station 6 Station 7 Station 8

Row 4

Station 9 Station 10 Station 11 Station 12 Station 13 Station 14 Station 15 Station 16

Row 3

Station 17 Station 18 Station 19 Station 20 Station 21 Station 22 Station 23 Station 24

Row 2

Station 31 Station 25 Station 26 Station 27 Station 28 Station 29 Station 30

Frontmost row
Figure Arrangement without Special Solution Stations

Row 4 Station 1 Station 2 Station 3 Station 4 Station 5 Station 6 Station 7 Station 8

Station 9 Station 10 Station 11 Station 12 Station 14 Station 16 Station 18 Station 20
Row 3
Station 13 Station 15 Station 17 Station 19
Row 2 Station 21 Station 22 Station 23 Station 24 Station 25 Station 26 Station 27 Station 28
Frontmost row Station 35 Station 29 Station 30 Station 31 Station 32 Station 33 Station 34
Figure Arrangement with Special Solution Stations

Fixed station numbers are assigned to the start stations (S1 to S3), end stations (linked), (E1-1 to E1-5),
end stations (not linked) (E2-1 to E2-5), wash stations (W1 to W4), drying stations (D1, D2) and link
station (LINK). The tables below specify the identification number of each station attribute and the
corresponding station number. The rightmost end station is selected as the PE station.

Start station S1 S2 S3 End station E1-1 E1-2 E1-3 E1-4 E1-5

Without special solution stations Station 30 Station 29 Station 28 Without special solution stations Station 25 Station 26 Station 27 Station 28 Station 29

With special solution stations Station 34 Station 33 Station 32 With special solution stations Station 29 Station 30 Station 31 Station 32 Station 33
* S2 and S3 can also be set as solution stations. * E1-2 to E1-5 can also be set as solution stations.

End station (not linked) E2-1 E2-2 E2-3 E2-4 E2-5

Without special solution stations Station 25 Station 26 Station 27 Station 28 Station 29

With special solution stations Station 29 Station 30 Station 31 Station 32 Station 33

* E2-2 to E2-5 can also be set as solution stations.

Link station LINK Wash station W1 W2 W3 W4

Without special solution stations Station 31 Without special solution stations Station 7 Station 6 Station 5 Station 4

With special solution stations Station 35 With special solution stations Station 7 Station 6 Station 5 Station 4

* The link station can be set only when the instrument is * W1 to W4 can also be set as solution stations.
connected to an automated coverslipper.

Drying station D1 D2
Without special solution stations Station 8 Station 16

With special solution stations Station 8 Station 20

* When a drying station unit is installed.

Special solution station SP1 SP2 SP3 SP4 SP5 SP6 SP7 SP8
Without special solution stations - - - - - - - -

With special solution stations Station 12 Station 13 Station 14 Station 15 Station 16 Station 17 Station 18 Station 19

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 Basics
● Arrangement of standard solution stations (with drying stations)
In the standard mode, the arrangement and number of stations vary depending on whether the solution
stations are arranged "without special solution stations" or "with special solution stations."

Station 1 Station 2 Station 3 Station 4 Station 5 Station 6 Station 7 Station 8

Row 4

Station 9 Station 10 Station 11 Station 12 Station 13 Station 14 Station 15 Station 16

Row 3

Station 17 Station 18 Station 19 Station 20 Station 21 Station 22 Station 23 Station 24

Row 2
Station 25
Station 32 Station 26 Station 27 Station 28 Station 29 Station 30 Station 31
Frontmost row

Figure Arrangement without Special Solution Stations

Station 1 Station 2 Station 3 Station 4 Station 5 Station 6 Station 7 Station 8

Row 4

Station 9 Station 10 Station 11 Station 12 Station 14 Station 16 Station 18 Station 20

Row 3
Station 13 Station 15 Station 17 Station 19
Station 21 Station 22 Station 23 Station 24 Station 25 Station 26 Station 27 Station 28
Row 2
Station 29
Station 36 Station 30 Station 31 Station 32 Station 33 Station 34 Station 35
Frontmost row

Figure Arrangement with Special Solution Stations

Fixed station numbers are assigned to the start stations (S1 to S3), end stations (linked) (E1-1 to E1-5),
end stations (not linked) (E2-1 to E2-5), wash stations (W1 to W4), drying stations (D1, D2), heating
stations (H1, H2) and link station (LINK). The tables below specify the identification number of each station
attribute and the corresponding station number. The rightmost end station is selected as the PE station.

Start station S1 S2 S3 End station (linked) E1-1 E1-2 E1-3 E1-4 E1-5
Without special solution stations Station 31 Station 30 Station 29 Without special solution stations Station 26 Station 27 Station 28 Station 29 Station 30

With special solution stations Station 35 Station 34 Station 33 With special solution stations Station 30 Station 31 Station 32 Station 33 Station 34
* S2 and S3 can also be set as solution stations. * E1-2 to E1-5 can also be set as solution stations.

End station (not linked) E2-1 E2-2 E2-3 E2-4 E2-5

Without special solution stations Station 26 Station 27 Station 28 Station 29 Station 30

With special solution stations Station 30 Station 31 Station 32 Station 33 Station 34

* E2-2 to E2-5 can also be set as solution stations.

Link station LINK Wash station W1 W2 W3 W4

Without special solution stations Station 32 Without special solution stations Station 7 Station 6 Station 5 Station 4

With special solution stations Station 36 With special solution stations Station 7 Station 6 Station 5 Station 4

* The link station can be set only when the instrument is * W1 to W4 can also be set as solution stations.
connected to an automated coverslipper.

Drying station D1 D2 Heating station H1 H2

Without special solution stations Station 8 Station 16 Without special solution stations Station 24 Station 25

With special solution stations Station 8 Station 20 With special solution stations Station 28 Station 29

Special solution station SP1 SP2 SP3 SP4 SP5 SP6 SP7 SP8
Without special solution stations - - - - - - - -

With special solution stations Station 12 Station 13 Station 14 Station 15 Station 16 Station 17 Station 18 Station 19

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 Explanation of Menu Screens
Stain Process Menu

[1] [2] [3]

[4] [5]

This menu screen appears while the instrument is standing by after logon.

[4] [2]

This menu screen appears during stain process.

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 Explanation of Menu Screens

Explanation of Icons in the Stain Process Menu

[1] Solution Configuration Selection

Touch this icon to switch the solution configuration used for staining. Select a desired solution
configuration from the stored configurations and apply it to the instrument.

[2] Staining Mode Selection

Touch this icon to set the following items regarding the starting of stain process. Once set, the staining
mode (continuous/batch) cannot be changed during stain process. Other settings can be changed
during stain process.

• Staining mode selection

Set whether to perform stain process in the batch mode or continuous mode.
• Initialization of Start Staining Screen
Set when to show the start staining screen. Select Opening the door or Touching START button.
• Initialization of Staining Process Start
Set when to start staining. Select Closing the door, Touching START button or Touching START
button after closing the door.

When the Closing the door is selected, be careful not to let stain process start inadvertently.

• Start Confirmation
Set whether or not to show a confirmation message at the start of staining.
• Process Start Notice
Set whether or not to notify the start of stain process by showing the start screen once staining
process has started.
• Heating Station Always Heated
When heating stations are used, set whether or not to always heat the heating stations.
• Solution Name Display
Set whether or not to display the applicable solution name (abbreviation) on each station in the
monitor screen showing a solution configuration.
• Link with Coverslipper
Set whet her to connect or disconnect the communication link with the connected coverslipper when
the link is set in the System Setup screen (P. 1-36). Even if the instrument is linked to a coverslipper,
slides are not transferred to the coverslipper if "Not in Use" is shown here. This item is displayed only
when the instrument is linked to an automated coverslipper.

[3] Reset Solution Usage Information

Touch this icon to reset the usage limit in runs/days/slides for each solution station whose usage
information is managed.

[4] Error Log

Touch this icon to check the error history and error descriptions or clear errors.

[5] Read Barcode (Optional)

Use this setting to read the 2D bar codes on Sakura staining solutions* and enter their expirations in
the instrument based on the 2D bar code information.

* Check the local Sakura Finetek website for availability of the Sakura staining solutions and staining
kits.

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 Explanation of Menu Screens
Utility Menu

Explanation of Icons in the Utility Menu

[1] System Setup

Touch this key to set date/time, set date/time formats, change passwords, set filter use limits, set the
institution name, set whether to enable or disable data export, adjust the alarm sound, and set whether
or not to output a key input sound. Touching this key displays the System Setup screen. (P. 1-36)

[2] Language Selection

Touch this key to switch the display language.

[3] Export Data

Select this key to export process reports, solution configurations, staining programs, solution
configuration name list, staining program name list, solution name list, system setup data and solution
usage status. (P. 1-43)

[4] Water Flow

Select this key to adjust the water flow rate to the wash stations. (P. 1-42)

[5] Arm Movement Check

Arm movement check is an operation to check for obstacles inside the moving range of the arm. If the
trays and solution reservoirs are not set correctly, the arm may contact them and cannot operate
correctly. If a problem is found in this check, set the accessories again correctly.

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 Explanation of Menu Screens
Edit Menu

Explanation of Icons in the Edit Menu

[1] Edit Solution Name

Select this icon to create a new solution name or edit an existing solution name. (P. 2-23)

[2] Edit Solution Configuration

Select this icon to create a new solution configuration or edit an existing solution configuration. (P. 2-
15)

[3] Edit Program

Select this icon to create a new staining program or edit an existing staining program. (P. 2-25)

[4] Program Check

Select this icon to check the solution configurations with which a given staining program can be run.
(P. 2-33)

[5] Delete Data

Select this icon to delete solution configurations, solution names, staining programs and process
reports. (P. 2-44)

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 System Setup
System Setup
In the System Setup screen accessible from the Utility Menu, you can define the basic settings needed to
use the instrument.

[9]
[1]

[2]
[10]
[3]

[4] [11]
[5]

[6] [12]

[7]

[8]

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 System Setup
[1] Date Format
This sets the format for the date display. Touching the option button toggles the display format in the
sequence of "Year/Month/Day," "Month/Day/Year" and "Day/Month/Year."

[2] Time Format

This sets the format for the time display. Touching the option button switches the time format between
"12 hour format" and "24 hour format."

[3] Key Sound

This selects whether to turn "ON" or "OFF" the sound output when a key is touched.

[4] Alarm Tone and Volume

This sets the tone and volume for system alarms. Touching the button toggles label on the button
"Loud and High," "Loud and Low," "Soft and High" and "Soft and Low."

[5] Extended Station Colors

Touching the button toggles label on the button between "Each Solution Name" and "Each Station".

[6] Export Data

This sets whether or not exporting data is possible and the destination path for data export. Touching
the button toggles label on the button between "File" and "OFF."

[7] Coverslipper Link

This button is used to set whether or not to enable the Coverslipper link.
This item is displayed only when the instrument is linked to an automated coverslipper.

[8] Coverslipper
If the Coverslipper link is installed, Coverslipper can be selected.

[9] Date and Time

You can set date and time. (P. 1-38)

[10] Password Input

You can manage user IDs and passwords. (P. 1-39)

[11] Fume Filter Management

You can manage filter use limits. (P. 1-41)

[12] Institution Name

You can set the name of the institution where the instrument is used.
Touching the Institution Name key displays the simple keyboard. Enter the applicable institution
name and then touch the Save key to save the name. An institution name can be entered using up to
20 characters. Touch the Caps Lock key to enter characters in uppercase. Touch the key again to
enter characters in lowercase.

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 System Setup
Setting Date and Time
You can set date and time by touching Date and Time from the System Setup screen accessible from the
Utility Menu.

Entering Year
Touching the Modify button on the right of "Year" displays the numeric keypad. Enter the current year
using the numeric keypad. You can enter a year in the range of 2000 to 2099.

Entering Month
Every time the ▲ or ▼ key is touched on the right of "Month," the selection changes to the next month
(▲) or previous month (▼).

Entering Day
Select a desired day by touching the corresponding number in the calendar.

Entering Hours and Minutes

You can enter hours and minutes using the numeric keypad displayed by touching the Modify button
below each item. Follow the time format selected by touching option button of "Time Format" (12 hour
format/24 hour format) from the System Setup screen.

am/pm
You can select am or pm only when the 12 hour format is currently selected.

After all items have been entered, touching the Save key to apply the settings.

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 System Setup
Setting User IDs, Passwords and Accessible Functions
You can set user IDs and passwords as well as the functions accessible by each user by selecting
Password Input from the System Setup screen accessible from the Utility Menu. To set each item, select
the applicable user and perform the corresponding operation explained below.

Setting an ID
You can change the existing ID of a user. Touching the User’s ID key displays the simple keyboard.
Enter a new user ID, and then touch the Save key to save the ID. A user ID can be entered using up to 20
characters. Touch the Caps Lock key to enter characters in uppercase. Touch the key again to enter
characters in lowercase.

Setting a Password
You can change a password only when you have logged on as an Administrator. Select the user whose
password you want to set, touch the Password Setup key, and then enter a new password consisting of
one to six digits on the numeric keypad. The entered password is shown in asterisks (*) in the password
field. After the password has been entered, touch the Enter key. When the screen prompts you to enter
the password again for confirmation, enter the same password and touch the Enter key again to save the
password.
Up to four "Administrator" passwords and 20 "User" passwords can be saved. The factory settings are
shown below.

Level Factory-set IDs Factory-set passwords

Administrator [ADMIN 1] ~ [ADMIN 4] [100000] ~ [400000]
User [OPERATOR 1] ~ [OPERATOR [000001] ~ [000020]
20]

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 System Setup
Setting Accessible Functions
You can set functions accessible by each user. This operation cannot be performed for "Administrators."

Stain Process
 Staining Mode Selection
Touching this function changes permissions for
"Staining Mode Selection" between Allowed and
Not Allowed.
 Abort Stain Process
Touching this function changes permissions for
"Abort Stain Process" between Allowed and Not
Allowed.
 Reset Solution Usage Information
Touching this function changes permissions for
"Reset Solution Usage Information" between
Allowed and Not Allowed.
 Solutions Configuration Selection
Touching this function changes permissions for
"Solutions Configuration Selection" between
Allowed and Not Allowed.

Utility
 System Setup
Touching this function changes permissions for
"System Setup" between Allowed and Not
Allowed.
 Export Data
Touching this function changes permissions for
"Export Data" between Allowed and Not Allowed.
 Language Selection
Touching this function changes permissions for
"Language Selection" between Allowed to Not
Allowed.
 Water Flow
Touching this function changes permission for
"Water Flow" between Allowed to Not Allowed.

Edit
 Edit Program
Touching this function changes permissions for
"Edit Program" between Allowed to Not Allowed.
 Program Check
Touching this function changes permissions for
"Program Check" between Allowed to Not
Allowed.
 Edit Solution Configuration and Solution
Name
Touching this function changes permissions for
"Edit Solution Configuration and Solution Name"
between Allowed to Not Allowed.
 Delete Data
Touching this function changes permissions for
"Delete Data" between Allowed to Not Allowed.

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 System Setup
Fume Filter Management
You can set items relating to fume filter management by selecting Fume Filter Management in the
System Setup screen accessible from the Utility Menu.

Whether or Not to Use Fume Filter Management

You can set whether or not to manage the use of fume filters. Touch the button to change between "In
Use" and "Not in Use."

Setting a Time Limit

Selecting "In Use" enables to touch the Set Time Limit key. When the Set Time Limit key is touched, you
can set a time limit for fume filters. Touching this key displays the numeric keypad, so enter a desired time
limit. To reset the entered time limit, touch the Clear key. A time limit can be set in the range of 1 to 999
hours in units of "hours." The default is "240" hours. After you have entered the time limit, touch the Enter
key to save the entry.
To maintain a clean work environment, it is recommended that the activated carbon filters (each set
consists of two filters) be replaced at least once every two weeks.

Resetting the Actual Time

Touch the Reset Actual Time button to initialize the present counter value of filter usage time. Touching
the button resets the actual time currently stored.

When all items have been set, touch the Exit key to return to the System Setup screen.

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 System Setup
Adjusting the Water Flow
You can change the run-up time for water supply to the wash stations by selecting Water Flow from the
Utility Menu.

• Water supply run-up time --- The time at which water supply to the wash stations is started during stain
process, prior to the transfer of tissues into the wash stations.

Change the setting whenever necessary before the start of staining, after changing the solution
configuration or staining program, and so on. The water supply run-up time must be changed after the
wash solution configuration has been changed. The water flow can be adjusted with the cover open.

1. Confirm that water has been drained from all wash stations.
2. Open the water supply handle.
3. Touch the Start key and wait for water to overflow from the wash stations.
4. Once water starts to overflow from the wash stations, touch the Stop key. Adjust the water supply
handle so that water will overflow within 20 to 30 seconds.
5. The water supply run-up time you have just set is displayed and the save screen appears. Touch Save
if you want to use this run-up time. Touching Exit returns to the Water Flow screen.

Water supply handle

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 Export Data
About Exporting Data
You can export the following items by selecting Export Data from the Utility Menu. If the output
destination is set to "OFF," export operation is disabled.

[1] [2] [3]

[4] [5] [6]

[5] [8] [9]

[1] Solution Configuration (Solution Configuration List)

Touch this button to export the details of solution configurations created by the user.

[2] Stain Program (Stain Program List)

Touch this button to export the details of staining programs created by the user.

[3] Process Result (Process Report)

Touch this button to export the results of staining runs. If an error or other disturbance occurred during
stain process, information of the applicable error is also exported.

[4] Solution Name List

Touch this button to export a list of all solution names created by the user.

[5] Solution Configuration Name List

Touch this button to export a list of names of all solution configurations created by the user.

[6] Stain Program Name List

Touch this button to export a list of names of all staining programs created by the user.

[7] System Setup (System Setup List)

Touch this button to export the details of system setup saved by the user.

[8] Solution Usage Status (Solution Usage List)

Touch this button to export the solution usage status for the stations managed by the user.

[9] Barcode History

A list of up to 1,000 sets of bar code information read by the user can be exported to an external file.

An example of data export is shown on P. 2-40. The heading in the parenthesis is the title when
exported..

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 Names of Files Exported to a CF Card
If the export destination is set to File, each set of output data is saved to a CF card as a file. The files of
each exported item are named in the applicable format shown below.

Exported item File name

Solution configuration CL*****.txt
Staining program PL*****.txt
Process report REP*****.txt
Solution name SNL*****.txt
Solution configuration name CNL*****.txt
Staining program name PNL*****.txt
System setup ISL*****.txt
Solution usage status SUL*****.txt
Bar code history BCH*****.txt

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 Chapter 2
Operating Procedure

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 Operating Procedure
Flow of Stain Process Operation
This section explains a series of operating procedures.

Flow of Stain Process Operation

A basic flow of normal operation is shown below.

● Preparation for Operation Standby Refer to P. 2-2 for details.

• Start the water supply.
• Turn on the power and log on to the instrument.

● Preparation for Staining Refer to P. 2-3 for details.

• Open the lid of each solution reservoir to check the solution level.
• Select a solution configuration.

● Start of Staining Refer to P. 2-5 for details.

• Select a staining program.
• Set staining baskets in the start station.
Staining
▼
Operations permitted during staining (P. 2-7)
To perform another staining run
consecutively, add staining baskets.
* Applicable only in the continuous mode.
(P. 2-7)
▼
● End of Staining Refer to P. 2-12 for details.
• Remove the stained baskets from the end station.

● End of Operation Refer to P. 2-12 for details.

• Turn off the power and stop the water supply.
• Close the lid of each solution reservoir.

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 Operating Procedure

Preparation for Stain Process Refer to P. 2-12 for details.

This section explains the preparation to be performed before the start of stain process.

Opening the Water Supply

1. Open the tap water faucet so that water can be supplied.
2. Hold the center of the cover and raise while pulling it toward you to open the cover and remove the lid
of each solution reservoir.

Be sure to remove all lids. If any lid remains closed, tissues may be damaged when the baskets are
transferred to the applicable station.

Logging On to the Instrument

1. The user must log on to the instrument to
start instrument operation. Only one user
can log on to the instrument at a time.
When the power switch located at the
bottom right of the instrument is turned on,
the display shows a message indicating
that the instrument is loading data. After a
while, a confirmation screen on instrument
use appears. After confirming the items,
touch the OK button to return to the Stain
Process Monitor screen.

Touch the Start button under "Confirm Water Supply," and you can perform a water supply test. Touch
the Stop button to stop the water supply.

2. When the Stain Process Monitor screen appears, touch the Log On key at the bottom left. When the
Enter Password sub-window appears, enter a pre-assigned password using the numeric keypad
(number keys 0 to 9) shown on the display. If you have made a mistake, touching the Backspace key
deletes the last character entered. Alternatively, you can touch the Cancel key to cancel the password
entry and return to the Stain Process Monitor screen. Refer to P. 1-39 for passwords.
3. After the password has been entered, touch the Enter key to complete the logon.

Logging off
To log off from the instrument, touch the Log Off key at the bottom left of the screen. You can log off
from the instrument even after staining has started. Once the user logs off, the instrument only accepts
the operation inputs to check the staining status, which prevents inadvertent operations by different
users. To perform other operations, the user must log on to the instrument again.

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 Operating Procedure

Selecting and Saving a Solution Configuration and Setting Solutions

The instrument can store multiple solution configurations and staining programs. Accordingly, you must
select a solution configuration appropriate for the staining program you want to run and display the
selected configuration on the monitor screen before starting the program. This section explains how to
select and save a solution configuration that will be displayed on the monitor screen.

1. Touch the Menu key shown at the bottom right of the Stain Process Monitor screen.
2. Select Stain Process Menu from the Stain Process Menu, Utility Menu or Edit Menu, and then touch
the Solution Configuration Selection key.
3. Select and touch a desired solution configuration name under Solution Configuration List, and then
touch the Select key. For creating a new station configuration or editing an existing station
configuration, refer to "Setting a Solution Configuration" (P. 2-15).

"Selecting a Solution Configuration" If any of the following conditions applies to a solution station for
which a solution management option has been set, the solution management information will not be
transferred.
• The station position where solution management is performed changes when the solution
configuration changes.
• The solution at the station position where solution management is performed changes to a different
solution when the solution configuration changes.
• The management method for the station where solution management is performed changes when
the solution configuration changes.
If the solution used, station position (station number) and management method do not change after
the solution configuration is changed, the solution management information will be transferred.

Touch the Check Program key to check the staining programs that can be operated with the solution
configuration you have selected.
To check a given solution configuration, select the applicable solution configuration and touch the
View key to display the Solution Configuration View screen showing the applicable station
arrangement. You can also check the reservoir arrangement of the next configuration by touching the
Change View key at the bottom right. If the current solution configuration is appropriate, touch the
Select key. To check other solution configuration, touch the Exit key and select a desired solution
configuration again.

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 Operating Procedure
4. Touching the Select key displays the New Solution Configuration screen. If the new solution
configuration you have selected requires changing the arm holder from the 3-position type to 1-position
type, or from the 1-position type to 3-position type, the Change Arm Holder window appears with the
message asking if the arm holder has been changed.
Change the arm holder according to the message, and then touch Yes. Refer to P. 1-14 for details on
how to change the arm holder.
5. Open the cover and set solutions according to the screen display. First, set solution reservoirs by
paying attention to the different reservoir types; i.e., standard solution reservoir, small solution reservoir
and special stain solution reservoir.
6. Next, fill solutions into the reservoirs. Solutions should be filled into the solution reservoirs beforehand
on a flat, stable surface, or directly into the reservoirs already set in the instrument. If solutions are
filled outside the instrument, transport the filled reservoirs into the instrument using special solution
trays to facilitate the operation.
7. After all solutions have been set, close the cover and touch the Exit key to save the selected solution
configuration.

To perform other operation, you can return to the Stain Process Menu screen by touching the Cancel key.

Setting Slides into Baskets

This section explains how to set slides into baskets.

1. Prepare slides to be stained. Check the solution configuration and prepare baskets (20-slide or 10-
slide baskets) together with appropriate basket adapters.
2. Attach a basket adapter to each basket by aligning the "UP SIDE" mark on top of the adapter with the
"UP SIDE" mark on one side of the basket. After confirming that both "UP SIDE" marks are facing the
same direction, tilt the basket adapter along the groove.
3. Next, store the slide into the basket one by one. Set the slides in the correct orientation so that their
tissue side aligns with the "UP SIDE" marks. When all slides have been set, return the tilted basket
adapter to the original position.

Do not use a deteriorated, worn, damaged or deformed basket/basket adapter, as it may cause
problems. Replace any such basket/basket adapter with a new one. Store the slides evenly in the
basket.

If the slides are stored unevenly, basket movements are affected during the staining operation.
Particularly when there are fewer slides, be careful not to let the basket tilt when storing the slides.

Tissue side "UP SIDE" mark "UP SIDE" mark

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 Operating Procedure

Start of Stain Process

This section explains a normal procedure to start stain process. Refer to P. 2-13 for the procedure to
start stain process in the "batch mode" by specifying the start step and end step.

1. Touch the Start key at the bottom right of the

Stain Process Monitor screen. If the Stain
Process Monitor screen is not currently displayed,
complete or abort the current operation and touch
the Exit key or Reset key at the bottom right of
the screen to return to the Stain Process Monitor
screen.

2. Touch the Start key and select a desired staining

program under Program List.
To check the details of the selected program,
touch View Program key at the bottom right.

3. Next, select the place to set a basket in, from

among the baskets shown under Available Start
Stations on the right side of the screen. If all start
stations are empty, "S1" is automatically selected.
To change the start station, select the new station,
and then touch the original station to cancel the
selection. You can also select and set multiple
start stations simultaneously for the same staining
program. The number of start stations as well as
the number and type of baskets that can be set in
each start station are fixed depending on the
selected "solution configuration." Check the
applicable basket type from the basket icon
shown on the screen.

If three start stations have been set, "S1," "S2"

and "S3" are shown. Touching a desired icon
on the screen adds the basket mark on the
applicable station, thus setting it as the start
station in which baskets are set.
For standard solution station
Up to three 20-slide baskets can be set. 4. If the program covers stations for which
"Management by slides" has been set, the field in
Use the 3-position arm holder. which to enter the number of slides is displayed. If
this field is displayed, enter a desired number of
For small solution station slides.
Only one 20-slide basket can be set. Touching the Set key displays the virtual numeric
keypad for entering values.
Use the 1-position arm holder and
attach a 20-slide basket The range of values that can be entered varies
load/unload adapter to the start depending on the program. If the program covers
station. The arm will not pick up only standard solution stations, the range is 1 to
baskets set in positions other 60. If the program covers only small solution
than the center of the start stations, the range is 1 to 20. If the program
station.
For special stain solution station covers special stain solution stations, the range is
Only one 10-slide basket can be set. 1 to 10.

Use the 1-position arm holder and If the Start button is touched without entering a
attach a 10-slide basket value, a warning window appears. If the
load/unload adapter to the start program does not cover any station for which
station. Stain process cannot be "Management by slides" has been set, the field
started if a 10-slide basket in which to enter the number of slides is not
load/unload adapter is not set. displayed. If "Management by slides" is
selected, processing can be started at only one
station at a time.

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 Operating Procedure
5. Open the door at the bottom of the cover to set a basket into the start station. Push the top of the
center of the door toward the back once opens the door toward you. When the door opens, draw out
the reservoir in the start station and set by paying attention to the orientation of the basket in which
slides have been set. Set the basket so that the "UP SIDE" mark on top of the basket adapter faces
right. Align the grooves in the top surface of the start station with the grooves provided at both ends of
the top surface of the basket adapter. After setting the basket, push the start station reservoir into the
instrument, close the door, and then push the door until a click sound is heard.

When setting a small solution reservoir or special stain solution reservoir, attach an applicable
adapter to the start station. If not, stain process cannot be performed correctly. Also confirm that the
arm holder has only one tab. If a wrong arm holder is set, stain process cannot be performed
correctly.

6. Touching the Start key at the bottom right of the screen displays a confirmation message. Select Start
to start staining.
7. Once staining starts, the Stain Process Monitor screen appears where you can check the current
processing. Refer to P. 2-7 for the operations permitted during stain process.

The starting procedure explained above is only one example. If the instrument has been set to start
staining upon closing of the door, you need not touch the Start key because closing the door
automatically starts stain process. Refer to P. 1-33 for the settings relating to the starting procedure.

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 Operating Procedure

Operations Permitted during Stain Process

The following operations are permitted during stain process. Addition of baskets and setting of priority start
are enabled only in the continuous mode.
• Add a basket
• Priority start
• Check the end station status
• Check the staining status
• Menu operations permitted during stain process
• Pause and abort stain process
• Remove a basket

Adding a Basket
This section explains the procedure to add a basket during stain process. This procedure is basically the
same as the normal staining start operation, but a basket can be added only in the continuous mode. Also
note that no basket can be added in the following conditions:
• The instrument is performing stain process in the batch mode.
• 11 staining runs are being processed.
• The start station is full.
• An error (including a station full error) is present.

1. Touch the Add Baskets key at the bottom right of the

Stain Process Monitor screen.
2. When the Stain Start Monitor screen appears, select a
desired staining program under Program List. To check
the details of the selected program, touch View
Program key at the bottom right.
3. Next, select the location of the start station to set a
basket in, from among the baskets shown under
Available Start stations on the right side of the screen.
Select S1, S2 or S3. Touching a desired station selects
it as the start station, if the station is available. To
change the start station, select the new station, and then
touch the original station to cancel the selection. You
can also select and set multiple start stations
simultaneously for the same staining program. The
number of start stations as well as the number and type
of baskets that can be set in each station are fixed
depending on the selected "solution configuration."
Check the applicable basket type from the basket icon
shown on the screen.
4. If the program covers stations for which "Management
by slides" has been set, the field in which to enter the
number of slides is displayed. If this field is displayed,
enter a desired number of slides.
Touching the Set key displays the virtual numeric
keypad for entering values.
The range of values that can be entered varies
depending on the program. If the program covers only
Start screen with the Glas g2 connected standard solution stations, the range is 1 to 60. If the
program covers only small solution stations, the range is
1 to 20. If the program covers special stain solution
stations, the range is 1 to 10.

If the Start button is touched without entering a value, a

warning window appears. If the program does not cover
any station for which "Management by slides" has been
set, the field in which to enter the number of slides is not
displayed.
When using a coverslipper, enter an applicable program
number in the coverslipper program number entry field.

Start screen with the Film connected

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 Operating Procedure
5. Open the door at the bottom of the cover to set a basket into the start station. Confirm that the arm is
not on a solution station in the frontmost row. If the arm is in the frontmost row when the door is
opened, an error will generate and the operation will pause, which may extend the staining time. Open
the door, draw out the start station reservoir, and then carefully set a basket in which slides have been
set, by paying attention to the orientation of the basket. Set the basket so that the "UP SIDE" mark on
top surface of the basket adapter faces right. After setting the basket, push the start station reservoir
into the instrument, close the door, and then push the door until a click sound is heard.

If you are using a 20-slide basket in a small solution station, attach a 20-slide basket load/unload
adapter to the start station. If not, staining process may not be performed correctly. If you are using a
special stain solution station, attach a 10-slide basket load/unload adapter to the start station. If not,
staining process will not be started.

6. Touching the Start key at the bottom right of the screen displays a confirmation message. Select Start
to start staining. If the instrument has been set to start stain process upon closing of the door, you
need not touch the Start key because closing the door automatically starts stain process. Refer to P. 2-
19 for the applicable settings.
7. Once staining starts, the Stain Process Monitor screen appears.

Setting Priority Start

Priority start is a function that allows tissues set in the start station later to be transferred and stained
before other tissues that are ready and standing by in the start station. The end time cannot be
forwarded. This function does not work if no basket is set in the start station.

1. Confirm that the target basket is set in the start station. Then, touch the Start key and select a desired
staining program from Program List in the Stain Process Monitor screen, just like when you are adding
a basket.
2. Touching the Priority key activates the priority start function and displays the Stain Process Monitor
screen.

Checking the End Station Status

You can check the statuses of all end stations currently defined, by touching the End Station Status key
in the Stain Process Monitor screen during stain process. The end stations are numbered E1-1, E1-2, and
so on, from the left, when the instrument is linked to a coverslipper, or E2-1, E2-2, and so on, from the left,
when the instrument is not linked to a coverslipper.
The end stations indicated by an asterisk (*) have no basket inside.
Touch the Exit key to return to the Stain Process Monitor screen.

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 Operating Procedure

Checking the Staining Status

You can check the status of the current staining run by touching the Detailed Monitor key in the Stain
Process Monitor screen during staining process. The scheduled end time can also be checked in the
Detailed Monitor. To return to the previous screen, touch the Overview Monitor key. You can log off from
either screen, and both screens function as the Stain Process Monitor screen.

Overview Monitor

Detailed Monitor

The staining run numbers and abbreviated staining program names shown in the "No." field are also
shown in the Overview Monitor, so the current step can be visually identified at a glance. Also, you can
touch the button corresponding to a desired staining run number (No.) in Overview Monitor to check
the details of the applicable staining program. Touch the Exit key to return to the Overview Monitor.

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 Operating Procedure
Menu Operations Permitted during Staining Process
You can perform certain menu operations during stain process by touching the Menu key in the Stain
Process Monitor screen.

● Error display
Up to 99 errors that have occurred since the start of staining process are displayed, from the latest
error shown on top.
You can touch the keys on the side of the screen to scroll the list to show the hidden columns.
Touching Error Description displays a screen explaining error numbers and corresponding error
descriptions. You can also touch Clear to clear the saved error data.

● Setting a staining mode

Once set, the staining mode (continuous/batch) cannot be changed during stain process. Other
settings can be changed during stain process. (P.1-33)

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 Operating Procedure

Pausing and Aborting Stain Process

This section explains the procedures to pause and abort stain process. The abort operation can be
performed only after the pause operation.

● Pause operation
To pause staining process, touch the Pause key on the left of the Stain Process Monitor screen.
Touching the Pause key displays the pausing window. The arm will continue to move until a position
where it can stop safety, after which all staining runs will stop.
To resume stain touch the Resume key. Note that the pause operation may make it no longer possible
to complete the process by the scheduled end time. In this case, the scheduled end time must be
extended at the time of resumption by up to the period during which stain process has been paused.

● Abort operation
To cancel all or part of the current staining runs, touch the Pause key in the Stain Process Monitor
screen and then touch the Abort key. When the abort window appears, touch the All Runs key to
cancel all staining runs. To cancel certain staining runs, touch the applicable staining run numbers to
add a check mark to the "Selected" field of each staining run.
When the Abort key is touched, a confirmation screen appears and asks if you want to abort the
selected staining runs. Selecting Yes displays the abort completion window. Open the cover and
remove the aborted baskets. Once the baskets have been removed and the cover closed, the Exit key
is displayed. Touch this key to complete the abort operation.

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 Operating Procedure
Removing a Basket during Stain Process
The following operation is performed when removing a basket from the end station or PE station during
stain process or after completion of stain process. It cannot be performed if the end station or PE station
contains no basket.

1. Confirm that the arm is not on a solution station in the frontmost row, and then open the door and
remove the basket.
2. Once the basket has been removed and the door closed, the basket removal confirmation window
appears. Touch the Yes key in this window. If you have not yet removed the basket, touch the No key.

End of Stain Process

Stained baskets are stored in the specified end station or PE station. (If the instrument is linked to the
Coverslipper, stained baskets are stored in the link station. If the link station is full, stained baskets are
temporarily stored in the end station and wait there until they can be stored in the link station.)

Removing Stained Baskets

1. A window notifying the end of stain process appears and automatically disappears in around 15
seconds.
2. Confirm that the arm is not on a solution station in the frontmost row, and then open the door and
remove the basket.
3. Once the basket has been removed and the door closed, the basket removal confirmation window
appears. Touch the Yes key in this window. If you have not yet removed the basket, touch the No key.

After the door is opened, remove all stained baskets from the end station and PE station.

End of Operation
This section explains the procedure to complete the stain process and end the operation.

1. After all operations have completed, turn off the power switch located at the bottom right of the
instrument, and then stop the water supply.
2. Close the lids on all solution reservoirs, and then close the cover.

This completes the series of operations.

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 Operating Procedure

How to Start Stain Process by Specifying the Start and End Steps
You can start stain process by specifying the start and end steps only in the batch mode. If a desired
step is specified as the start step, the staining program will start from the specified step. If a desired step
is specified as the end step, staining process will end at the specified step and the baskets will be
transferred to the end station specified in the applicable staining program.

The start and end steps only represent temporary changes in the start and end stations. The settings
will become ineffective once the series of staining runs are completed or aborted. The start and end
steps cannot be set on the stations shown in the table below.
If the start step is set on a drying station, the drying station heater is turned on at the start of staining.
If the start step is set on a heating station, the baskets are held in the start station until the heating
station reaches the specified temperature.

Stations that cannot be set as the start step Stations that cannot be set as the end step
End stations, PE station Start stations, End stations, PE station

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 Operating Procedure
1. Touch the Start key at the bottom right of the Stain Process Monitor screen. If the Stain Process
Monitor screen is not currently displayed, complete or abort the current operation and touch the Exit
key or Reset key at the bottom right of the screen to return to the Stain Process Monitor screen.

2. Touch the Start key and select a desired staining program under Program List.
3. Next, set a basket and select a start station under Available Start stations. Refer to P. 2-4 for details on
how to set a basket and select a start station. Once the basket has been set and start station selected,
touch the View Program key at the bottom right.
4. The View Program screen appears and the details of the currently selected staining program are
displayed. Touch the Start/End Step key at the bottom right.
5. The Select Start/End Steps screen appears.
6. To set a start step, select a step number you want to specify as the start step, and then touch the Start
Step key to the right. When the key is touched, "S" appears in front of the step number to indicate that
it has been set as the start step.
7. To set an end step, select a step number you want to specify as the end step, and then touch the End
Step key to the right. When the key is touched, "E" appears in front of the step number to indicate that
it has been set as the end step.
8. Touching the Save & Start key starts stain process. Touching the Cancel key cancels the start/end
step settings and returns to the View Program screen.

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 Setting a Solution Configuration
From "Solution Configuration," you can set a configuration of necessary solution stations and other items
that are needed to run the staining program smoothly.

Creating a New Solution Configuration

This section explains the procedure to create a new solution configuration. Up to 50 solution
configurations can be saved.

Saving a Configuration Name

1. Touch Menu at the bottom right of the Stain Process Monitor screen, and then select Edit Solution
Configuration from the Edit Menu.
2. Touch Add Configuration, and then enter a solution configuration name (using of up to 20 characters)
from the simple keyboard shown in the Create Solution Configuration Name screen.
After the solution configuration name has been entered, touch the Save key to save the name.
3. Touching the Save key displays the Solution Configuration Setup window (P. 2-16).

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 Setting a Solution Configuration
Setting Up a Solution Configuration
Setting a solution configuration name displays the Solution Configuration Setup window. In this screen, the
entire solution configuration can be set. The setting of each item is explained below.

● Solution Configuration Type

You can select whether to use standard solution reservoirs (Standard) or small solution reservoirs
(Small). Touching this function toggles, the selection between Standard and Small.

● Number of Start Stations

Determine the number of start stations. Up to three start stations can be set. Touching this function
toggles in the sequence of #1 Station, #2 Stations and #3 Stations.

● Program End (PE) Station

Select whether or not to use a PE station. If a PE station is used, the PE station is set on the rightmost
end station. Touching this function toggles the selection between In Use and Not In Use.

● Setting Linked End Stations (E1)

If the instrument is linked to a coverslipper, the processing moves to the linked end stations when the
staining ends. Up to five such stations can be set. Touching this function toggles the selection in the
sequence of Not In Use, 1 Station, 2 Stations, 3 Stations, 4 Stations and 5 Stations.

● Setting Unlinked End Stations (E2)

Determine the number of end stations that are not linked to the coverslipper. Up to five such stations
can be set. Touching this function toggles the selection in the sequence of Not In Use, 1 Station, 2
Stations, 3 Stations, 4 Stations and 5 Stations.

Set no more than six stations including the start station, PE station, linked end stations (E1) and
unlinked end stations (E2).

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 Setting a Solution Configuration

● Number of Wash Stations

Determine how many wash stations to be used or no wash station will be used. If used, up to four
wash stations can be set. Touching this button toggles in the sequence of Not In Use, 1 Station, 2
Stations, 3 Stations and 4 Stations.

● Special Solution Stations

Determine whether or not to use special solution stations. Touching this function toggles the selection
between In Use and Not In Use.

● Heating Stations
Determine whether or not to use heating stations. If Not In Use (No Heating) is selected, the heating
stations do not perform heating control and are treated as normal special stain solution stations.
Touching this function toggles the selection in the sequence of In Use, Not In Use and Not In Use (No
Heating).

Touching the Save key displays the next setup screen, or Edit Solution Configuration screen, where
you can set each solution station.

Setting an Edit Solution Configuration

Setting the items in the Solution Configuration Setup screen and then touching the Save key displays the
Edit Solution Configuration screen. In this screen, you can set detailed settings for individual solution
stations. The available settings are explained below. After the necessary items have been set, touch the
Save key to save the completed configuration.

• Solution Configuration Layout

You can touch each station to set a solution name for the specified solution station as well as
management method of the solution. These items can be set only for colored solution stations. Those
filled with gray do not permit these settings.
• List
You can display the solution configuration in a list format.
• Properties
Touching Properties in the Edit Solution Configuration screen opens another screen where you can set
display colors for the selected solution configuration as well as items relating to staining control.
…Setting Station Colors (P.2-19)
Change the display color of each station group.
…How to Set Staining Control Data (P.2-20)
You can set detailed controls such as "Mix," "Enhanced Wash," "Basket Lifting Speed," "Water Wash
Cycle Time" and "Temperature Settings."

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 Setting a Solution Configuration

● How to Set Solution Name and Management Method

You can touch each station to set a solution name for the specified solution station as well as
management method of the solution. These items can be set only for colored solution stations. Those
filled with gray do not permit these settings.

1. In the Edit Solution Configuration screen, touch the station you want to set a solution for. Select a
desired solution name from "Standard Solutions," "Sakura Solutions * " and "Kit Solutions * " in the
solution setting field.

* Check the local Sakura Finetek website for availability of the Sakura staining solutions and staining
kits.

Selecting "Standard Solutions" displays solution names of some standard solutions and solution
names registered by user. Refer to P.2-23 for how to create or edit solution names.
Selecting "Sakura solutions" displays solution names of Sakura staining solutions managed by bar
code.
Selecting "Kit solutions" displays the solutions that constitute the Sakura staining kits managed by
bar code.

2. After selecting the solution name, set the solution management method.
Touching the Management Method toggles in the sequence of "Runs: Management by runs
done/Days: Management by days/Slides: Management by slides/NONE: Not in Use."

3. Next, determine a specific usage limit. Touching the Set button in the entry field displays the virtual
numeric keypad, so enter a setting value using the numeric keypad.

Runs: Manages the number of runs. You can enter a value between 0 and 99.
Days: Manages the number of days. You can enter a value between 0 and 99.
Slides: Manages the number of slides. You can enter a value between 1 and 9999.
NONE: Not in Use. The entry field is grayed out.

4. Touching the Save key saves the settings and returns to the Edit Solution Configuration screen.

● How to Set Station Groups

Select solution names and touch the Group button, and you can assign the stations to groups G1 to
G5.
The stations in a group can have a colored border.

● Switching to List View

You can check the current solution configuration in a list format by touching Detailed View in the Edit
Solution Configuration screen.

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 Setting a Solution Configuration
● How to Set Station and Group Colors
Touching Properties in the Edit Solution
Configuration screen opens another screen where
you can set display colors for the selected solution
configuration as well as items relating to staining
control.

● Setting Station Colors

You can assign colors to the solution stations,
heating stations, special solution stations, drying
stations, wash stations, start stations, end stations,
and PE station, except for the link stations.
Fifty different colors are available.
Different colors can be set for each solution name or
station.

1. Touch the Properties button on the Edit Solution

Configuration screen, and then touch the Set Color
button on the Set Station Configuration Properties
screen, to move to the Set Station Color screen.
2. Touch the button corresponding to the station you
want to set a color for.
3. The Select Color window appears. Select one color,
and then touch the "Select" button to apply the
selection.
4. The Select Station Color screen closes and the color
of the applicable station button changes to the
selected color.
Touch the Save button to save the setting.

If the color setting mode is different for each

solution name, all stations of the same solution
name have the same color. Note that this only
applies to the solution stations, heating stations
and special solution stations.
The colors already selected are denoted by x.

● How to Set Colors for Solution Name Groups

The color setting screen for a group of solution
names has up to five buttons of G1 to G5. Eight
colors are available.

1. Touch the Properties button on the Edit Solution

Configuration screen, and then touch the Set Color
button on the Set Solution Configuration Properties
screen, to move to the Set Station Color screen.
2. Touch the button corresponding to the group whose
color you want to change. Example) G1 button
3. The Select Group Color window appears. Select one
color, and then touch the "Select" button to apply the
selection.
4. The selected color appears around the border of the
stations buttons in the group.
Touch the Save button to save the setting.

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 Setting a Solution Configuration
● How to Set Staining Control Data
At staining control data, you can set detailed staining controls by touching Mix, Enhanced Wash,
Basket Lifting Speed, Water Wash Cycle Time and Temperature Settings. These items can be set
separately for each solution configuration.

Mix Parameter Setup

• Mix Amplitude
You can set the amplitude of up/down basket
movement in the solution. Select a desired slider
and move it to right or left to change the setting.
The adjustable range is "10 to 30" (mm) at 5-mm
intervals. The default is "30" mm.
• Mix Frequency
You can set the mix frequency applicable to
each mixing operation. Select a desired slider
and move it to right or left to change the setting.
The adjustable range is "1 to 10" (times). The
default is "5."
• Mix Speed
You can set the speed of up/down basket
movement during mixing. Select a desired slider
and move it to right or left to change the setting.
The adjustable range is "1 to 7," with "7"
representing the maximum speed. The default is
"3."

Enhanced Wash Parameter Setup

• Enhanced Wash Frequency
You can set the number of times the up/down
basket movement out of the solution and back
into the solution is repeated. Select a desired
slider and move it to right or left to change the
setting. The adjustable range is "1 to 10" (times).
The default is "5."
• Enhanced Wash Speed
You can set the speed of up/down basket
movement during enhanced wash. Select a
desired slider and move it to right or left to
change the setting. The adjustable range is "1 to
5," with "5" representing the maximum speed.
The default is "3."

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 Setting a Solution Configuration

Basket Lifting Speed and Water Wash Cycle Time

Parameter Setup
• Basket Lifting Speed
You can set the speed at which baskets are lifted.
Select a desired slider and move it to right or left to
change the setting. The adjustable range is "1 to 6,"
with "6" representing the maximum speed. The
default is "1." Use "1" in normal conditions of use.

When the pull-up speed is increased, more

solution will be brought into the next station and
accelerate solution deterioration, resulting in
lower staining quality. There are also risks of
tissue contamination due to dropping solution.

• Water Wash Cycle Time

You can set the cycle at which the dirty water
containing staining solution and other contaminants
in the wash stations is changed. Set a value using
the numeric keypad displayed by touching the
Modify key.
The adjustable range is "30 to 90" (seconds). The
default is "60" (seconds).

Temperature Settings
• Drying Station Temperature
You can set the temperature of drying stations.
Set a value using the numeric keypad displayed by
touching the Modify key. The adjustable range is "30
to 65" (C). The default is "65" (C). Note that the
drying stations may not be controlled at low
temperatures depending on the outside air
temperature.

• Heating Station 1 Temperature

You can set the temperature of the heating station
installed at the back of the instrument. Set a value
using the numeric keypad displayed by touching the
Modify key. The adjustable range is "30 to 70" (C).
The default is "60" (C). Note that this heating station
may not be controlled at low temperatures depending
on the outside air temperature.

• Heating Station 2 Temperature

You can set the temperature of the heating station
installed at the front of the instrument. Set a value
using the numeric keypad displayed by touching the
Modify key. The adjustable range is "30 to 70" (C).
The default is "60" (C).
Note that this heating station may not be controlled at
low temperatures depending on the outside air
temperature.

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 Setting a Solution Configuration
Editing a Solution Configuration
This section explains the procedure to edit an existing solution configuration.

1. Touch Menu at the bottom right of the Stain Process Monitor screen.
2. Select Edit Solution Configuration in the Edit Menu.
3. Under Solution Configuration List in the Solution Configuration Selection screen, touch and select the
name of the solution configuration you want to edit.
4. Next, touching the Edit key displays the Create Solution Configuration Name screen showing the
simple keyboard. To change the solution configuration name currently saved, enter a new name from
the onscreen keyboard (using of up to 20 characters). After the new solution configuration name has
been entered, touch the Save key to save the name.
5. Touching the Save key displays the Solution Configuration Setup screen explained on P. 2-16.

If a solution configuration affecting any saved staining program has been edited, the changes cannot
be saved.

"Editing a Solution Configuration"

If any of the following conditions applies to a solution station for which a solution management option
has been set, editing the solution configuration resets the solution management information.
• The station position where solution management is performed is changed by editing the solution
configuration.
• The solution at the station position where solution management is performed is changed to a
different solution by editing the solution configuration.
• The management method for the station where solution management is performed is changed by
editing the solution configuration.
Note that, so long as each station where solution management is performed is not edited by editing
the solution configuration, and so long as the solution used, station position (station number) and
management method remain unchanged, the solution management information will not be reset even
when the solution configuration is edited.

Copying a Solution Configuration

This section explains the procedure to copy a solution configuration.

1. Touch Menu at the bottom right of the Stain Process Monitor screen.
2. Select Edit Solution Configuration in the Edit Menu.
3. Under Solution Configuration List in the Solution Configuration Selection screen, touch and select the
name of the solution configuration you want to copy and then touch the Copy key. The Copy Solution
Configuration screen appears.
4. Next, select the copying destination. To create a new solution configuration to copy to, touch the Add
Configuration key. To copy to an existing solution configuration, select the desired solution
configuration from the Copy to window and then touch the Copy key.
5. Touching the Add Configuration or Copy key displays the Create Solution Configuration Name
screen showing the simple keyboard. To change the solution configuration name, enter a new name
from the onscreen keyboard (using up to 20 characters). After the new solution configuration name has
been entered, touch the Save key to save the name.
6. Touching the Save key displays the Solution Configuration Setup screen explained on P. 2-16.

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 Setting a Solution Configuration

Editing a Solution Name

This section explains the procedure to set a solution name. Up to 100 solution names can be saved.
Each solution name must consist of up to 20 alphanumeric characters.

Creating and Editing a Solution Name

1. Touch Menu at the bottom right of the Stain Process Monitor screen.
2. Select Edit Solution Name from the Edit Menu.
3. When the Edit Solution Name screen appears, touch the Add Solution Name key if you want to create
a new solution name. To change an existing solution name, select the solution name you want to
change under Solution List, and then touch the Edit key.
4. Touching the Edit key displays the Create Solution Name screen showing the simple keyboard, so
enter a solution name (using up to 20 characters). Once the solution name has been entered, touch
the Save key. When the screen prompts you to enter an abbreviated solution name, enter an
abbreviation from the onscreen keyboard and then touch the Save key to save the abbreviation. (Enter
the abbreviation using up to eight characters.)
5. Touching the Save key displays the Edit Solution Name screen, where the solution name you have just
saved is shown under Solution List.

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 Setting a Solution Configuration
Copying a Solution Name
1. Touch Menu at the bottom right of the Stain Process Monitor screen.
2. Select Edit Solution Name from the Edit Menu.
3. Under Solution List in the Edit Solution Name screen, touch and select the solution you want to copy
and then touch the Copy key.
4. Touching the Copy key displays the Create Solution Name screen showing the simple keyboard. To
change the solution name, enter a new name from the onscreen keyboard (using of up to 20
characters). Once the solution name has been entered, touch the Save key. When the screen prompts
you to enter an abbreviated solution name, enter an abbreviation from the onscreen keyboard and then
touch the Save key to save the abbreviation. (Enter the abbreviation using up to eight characters.)
5. Touching the Save key displays the Edit Solution Name screen, where the solution name you have just
saved is shown under Solution Name List.

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 Setting a Staining Program
"Staining programs" are programs that automatically perform stain process based on preset "solution
configurations." Refer to P. 2-15 for "solution configurations."

Creating a New Staining Program

This section explains the procedure to create a new staining program from a preset "solution
configuration." If there is no "solution configuration" appropriate for the staining program you are creating,
refer to "Setting a Solution Configuration" on P. 2-15.
Up to 50 staining programs can be saved.

Saving a Staining Program Name

1. Touch Menu at the bottom right of the Stain Process Monitor screen.
2. Select Edit Program from the Edit Menu.
3. Touching Change Solution Configuration displays the Select Configuration window. In the list of
preset solution configurations, touch the solution configuration you want to use for your new staining
program and then touch the Select key.
4. Next, touch the Add Program key. When the Create Program Name screen showing the simple
keyboard appears, enter a solution name (using up to 20 characters). Once the solution name has
been entered, touch the Save key. When the screen prompts you to enter an abbreviated staining
program name, enter an abbreviation from the onscreen keyboard and then touch the Save key to
save the abbreviation. (Enter the abbreviation using up to five characters.)
5. Touching the Save key proceeds to the operation explained under "Creating a Staining Program" on P.
2-26.

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 Setting a Staining Program

Creating a Staining Program

To create a staining program, touch a desired item or select a desired location using the cursor keys
located at the bottom right. The procedure to create each item is explained below.

● How to Operate the "Step" Field

"Step" refers to the order of each staining step. You can touch and select a number(s) or blank field(s)
under "Step" in the Edit Program screen and then select Copy, Paste, Replace All Step, Insert Step
or Delete Steps to perform the applicable operation.

• Copy
Touching this button copies the line corresponding to the selected "Step" field. The data is not yet
pasted.
• Paste
Touching this button pastes the data in the copied line to the line corresponding to the "Step" field
currently selected. This operation inserts the source line immediately above the "Step" line currently
selected by the cursor. Basically, only the "Time," "Delay" and "Mix" data are copied.
• Replace All Steps
Touching this button replaces the "Time," "Delay" and "Mix" data of all lines excluding the start
station, end station and PE station lines, with the corresponding data in the copied line. The solution
station names are not replaced.
• Insert Step
Touching this button inserts a new blank "Step" field directly below the line corresponding to the
selected "Step" field.
• Delete Step
Touching this button deletes the line corresponding to the selected "Step" field.

Touching the Save key saves the applicable changes and closes this screen.

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 Setting a Staining Program

● How to operate the "Station" field

"Station" refers to each station number assigned to the currently selected solution configuration. You
can touch and select each "Station" field in the Edit Program screen and then select Select Station or
Program End Station to perform the applicable operation.

• Select Station
You can select the station to be assigned to the selected "Station" field.
Touching the Select Station key displays the solution configuration layout. When a desired station is
touched, the data of that station is displayed at the bottom of the screen to let you check the attribute
and solution name for the station. Touching the Modify key reflects the selection in the "Station" field
in the Edit Program screen, with the selected station number shown in the "Station" field and
applicable solution name shown in the "Solution Name" field. If a non-solution station such as start
station, end station, wash station or drying station is selected, the text displayed in the "Station" field
conforms to the table below.

Set an end station or stations at the end of the staining program.

• Program End Station

You can insert a PE station as a standby station in the selected line. This operation adds "PE" to the
"Station" field in the Edit Program screen. For your information, the PE station is automatically set to
the rightmost end station.

Touching the Save key saves the applicable changes and closes this screen.

Text displayed in
Text displayed in
Station attribute "Station" field
"Solution Name"
field
Start stations S* (Solution name)
End stations (linked) E1- * (Solution name)
End stations (not
E2- * (Solution name)
linked)
Wash stations W* Wash Station
Drying stations D* Drying Station
PE station PE (Solution name)

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 Setting a Staining Program

● How to operate the "Time" field

In the "Time" field, you can set a processing time for the currently selected solution. Set a desired time
in the "Hours: Minutes: Seconds" format. The maximum time that can be entered is "99:59:59."

• Modify
Touch the Modify key to determine a processing time. Touching this key displays the numeric
keypad where you can set a desired time in the "Hours: Minutes: Seconds" format. The input range
of each segment is shown below:

Hours: 0 to 99
Minutes: 0 to 59
Seconds: 0 to 59

Touching Clear clears the selected segment to "0." Touching the Enter key applies the entered
processing time. You can enter "0:00:00" in some fields. But if "0:00:00" is set in all fields, you cannot
save.

Touching the Save key saves the applicable changes and closes this screen.

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 Setting a Staining Program

● How to operate the "Delay" field

"Delay" refers to an "allowable delay" to be applied to a staining run in order to run another staining run
when multiple staining runs must be performed at the same time in the continuous mode while delaying
other stain process.
In the "Delay" field, you can set this "allowable delay" as one of four levels.

• Exact
No delay is allowed and each staining run is performed exactly according to the specified time.
• Limited (20%)
A delay of up to 20% of the specified time is allowed.
• Limited (50%)
A delay of up to 50% of the specified time is allowed.
• Unlimited
An unlimited delay is allowed.

Each setting is displayed in the "Delay" field as follows.

Touching the Save key saves the applicable changes and closes this screen.

Delay setting Text displayed in "Delay" field

Exact ＝＝
Limited (20%) 20％
Limited (50%) 50％
Unlimited ＊ ＊

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 Setting a Staining Program

● How to operate the "Mix" field

In the "Mix" field, you can set whether to perform "mixing" and "enhanced wash" during staining. Use of
these operations that closely simulate manual staining prevents mottled colors and co-staining to
enhance the staining quality.

• Mix
Touching the Mix key displays the screen where you can set mixing.
• Enhanced Wash
Touching the Enhanced Wash key displays the screen where you can set enhanced wash.
• OFF
Touching the OFF key disables both mixing and enhanced wash.

Each setting is displayed in the "Mix" field as follows.

Touching the Save key saves the applicable changes and closes this screen.

Mixing setting Text displayed in "Mix" field

Mix ON
Enhanced wash EW
OFF OFF

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 Setting a Staining Program
Editing a Staining Program
This section explains the procedure to edit an existing staining program.

1. Touch Menu at the bottom right of the Stain Process Monitor screen.
2. Select Edit Program from the Edit Menu.
3. Under Program List in the Select Program screen, touch and select the name of the staining program
you want to edit. The solution configuration used for the selected staining program is displayed on the
right. To change this solution configuration, touch the Change Solution Configuration key at the
bottom and select a new solution configuration under Solution Configuration List. To use the displayed
solution configuration, proceed to the next step.
4. Next, touch the Edit key. The Create Solution Configuration Name screen with the simple keyboard
appears. To change the staining program name currently saved, enter a new name from the onscreen
keyboard (using up to 20 characters). Once the staining program name has been entered, touch the
Save key. When the screen prompts you to enter an abbreviated staining program name, enter an
abbreviation from the onscreen keyboard and then touch the Save key to save the abbreviation. (Enter
the abbreviation using up to five characters.)
5. Touching the Save key proceeds to the operation explained under "Creating a Staining Program" on P.
2-26.

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 Setting a Staining Program
Copying a Staining Program
This section explains the procedure to copy a staining program.

1. Touch Menu at the bottom right of the Stain Process Monitor screen.
2. Select Edit Program from the Edit Menu.
3. Under Program List in the Select Program screen, touch and select the name of the staining program
you want to copy, and then touch the Copy key. The Copy Program screen appears.
4. Next, select the copying destination. To create a new solution configuration to copy to, touch the Add
Configuration key. To copy to an existing program, select the desired program from the Copy to
window and then touch the Copy key.
5. Touching the Add Program or Copy key displays the Create Solution Configuration Name screen
showing the simple keyboard. To change the staining program name, enter a new name from the
onscreen keyboard (using up to 20 characters). Once the staining program name has been entered,
touch the Save key. When the screen prompts you to enter an abbreviated staining program name,
enter an abbreviation from the onscreen keyboard and then touch the Save key to save the
abbreviation. (Enter the abbreviation using up to five characters.)
6. Touching the Save key proceeds to the operation explained under "Creating a Staining Program" on P.
2-26.

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 Setting a Staining Program
Checking a Staining Program
This section explains the procedure to check if a given combination of solution configuration and staining
program is feasible.

1. Touch Menu at the bottom right of the Stain Process Monitor screen.
2. Select Program Check from the Edit Menu.
3. When the Program Check screen appears, select the solution configuration you want to check under
Solution Configuration List. Next, select the staining program you want to check under Program List.
4 After both the program and solution configuration have been selected, touch the Check key at the
bottom right. The "Program Check Result" screen appears.
5. This screen shows problems with the selected combination, if any. If necessary, change the staining
program and/or solution configuration by referring to the displayed check result to correct the
problems.

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 How to Use the Bar Code Reader, and It's Function (Options)
Overview
Use the bar code reader connected to the instrument to read the bar codes, so that the expiration dates
and lot numbers of the staining solutions* will be entered in the instrument and reports of such solution
usage information, etc., will be generated. Also, bar code management of solution information enables the
following items:
• Store the solution usage information in the bar code of each solution used, so that an alarm is issued
when the unopened solution expires or the opened solution expires, whichever occurs first.
• An alarm is issued at the end of operation, even on the expiration date.
(The open date is the day when the bar code is read.)

Applicable Solutions
- Sakura staining solutions*
- Staining kits*
* Check the local Sakura Finetek website for availability of the Sakura staining solutions and staining kits.

How to Use Sakura Staining Solutions

Editing a Solution Configuration

1. When a special solution is used, select Edit Solution Configuration [1] on the Edit Menu, to move to
the Solution Configuration Setup screen.
2. On the Solution Configuration Setup screen, click the Save button [2] to move to the Solution
Configuration screen.

[1]

[2]

Edit Menu Screen Solution Configuration Setup Screen

3. Select the station where the special solution will be used, and select Sakura Staining Solutions [3].
When the Select Solution window [4] appears, select the special solution to be used. When the
solution is selected, the station is recognized as a Sakura special solution station.
(The details of each category are shown in the table below.)

Category Description
Standard solutions Solutions other than Sakura staining solutions (including user-defined solutions)
Sakura solutions Sakura staining solutions managed by bar code
* "*" is attached at the beginning of the solution name.
Solutions that constitute the Sakura staining kits managed by bar code
Kit solutions
* "/" is attached at the beginning of the solution name.

[4]

[3]

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 How to Use the Bar Code Reader, and It's Function (Options)
How to Enter Sakura Solution Bar Code Data
1. Prepare the solution to be set to the instrument.
2. Touch the Read Barcode button on the Stain Process Menu.
3. Move to the Read Barcode screen, and confirm the station for which the special solution is set.
Only the stations for which bar code can be entered are displayed.
The bar code reading procedure and solution state vary depending on the station/text color, so use the
table below as reference.

Station color
Sakura staining
Yellow
solution station
Kit staining solution
Green
station

Solution name color

Black Unexpired solution
Purple Solution expiring that day
Solution whose bar code
Red is not yet entered, or
expired solution

4. Select the applicable station and read the solution bar

code.
5. Once the bar code of the special solution has been read,
the information of the special solution is displayed on the
screen.

6. If the information displayed on the screen is correct, set

the special solution to the specified station.

The special solution pouch is easy to deform, so use

one hand to orient its solution outlet toward the
station. Also, squeezing the pouch causes the
solution to gush out, so gently hold the pouch with
the other hand.

7. Once the solution has been set, touch the Enter button.
8. Touch the Save button and then touch the Yes button in
the figure below, and the entered bar code information
will be saved.

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 How to Use the Bar Code Reader, and It's Function (Options)
How to Use Sakura Staining Kit

Editing a Solution Configuration

1. When a staining kit is used, select Edit Solution Configuration [1] on the Edit Menu, to move to the
Solution Configuration Setup screen.
2. On the Solution Configuration Setup screen, touch the Select Kit button [2] to display the Select
Staining Kit window, and then set the staining kit used for the solution configuration.

[1]

[2]
Edit Menu Screen Solution Configuration Setup Screen

3. For the specified station, select Kit Solutions [4] from the categories shown in [3].
(The details of each category are shown in the table below.)

Category Description
Standard solutions Solutions other than Sakura staining solutions (including user-defined solutions)
Sakura staining solutions managed by bar code
Sakura solutions
* "*" is attached at the beginning of the solution name.
Solutions that constitute the Sakura staining kits managed by bar code
Kit solutions
* "/" is attached at the beginning of the solution name.

Touching the Kit Components button [4] displays the Select Solution Kit Components window [5],
so select the solution you want to assign to the station. When the solution is selected, the station is
recognized as a kit component solution station.

[5]
[3]

[4]

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 How to Use the Bar Code Reader, and It's Function (Options)
How to Enter Kit Component Solution Bar Code Data
1. Prepare the solution to be set to the instrument.
2. Touch the Read Barcode button on the Stain Process Menu.
3. Move to the Read Barcode screen, and confirm the station for which the special solution is set.
Only the stations for which bar code can be entered are displayed.
The bar code reading procedure and solution state vary depending on the station/text color, so use the
table below as reference.

Station color
Sakura staining
Yellow
solution station
Kit staining solution
Green
station

Solution name color

Black Unexpired solution
Solution expiring that
Purple
day
Solution whose bar
Red code is not yet entered,
or expired solution

4. Select the station for which the kit is set, and read the bar
code of the kit.
5. Once the kit has been read, the information of the kit is
displayed on the screen.
If the information displayed on the screen is correct,
touch the Enter button.

6. The bar code of the kit component solution is read.

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 How to Use the Bar Code Reader, and It's Function (Options)

7. Once the bar code of the solution has been read, the
information of the solution is displayed on the screen, as
with the kit.

8. If the information displayed on the screen is correct, set

the station containing the special solution as the specified
station in the instrument.

The special solution pouch is easy to deform, so use

one hand to orient its solution outlet toward the
station. Also, squeezing the pouch causes the solution
to gush out, so gently hold the pouch with the other
hand.

9. Once the solution has been set, touch the Register

button. Touch the Register button and then touch the Yes
button in the figure below, and the entered bar code
information will be saved.

10. When the Yes button is touched, the station to which the
bar code information has been set turns blue. In the case
of a kit, read all solutions constituting the kit and then
touch the Register button in the bottom right of the
screen, to complete the solution saving process.

The Register button becomes selectable after all of

the solution constituting the kit have been read.
Returning to the previous screen without touching
the Register button resets the saved information.

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 How to Use the Bar Code Reader, and It's Function (Options)
Resetting the Solution Usage Information
You can select the Reset Solution Usage Information screen from the Stain Process Menu and check for
expired solutions and reset their usage information.
You can also move to the Read Bar Code screen from the Reset Solution Usage Information screen.

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 How to Use the Bar Code Reader, and It's Function (Options)
Outputting Process Report
Each report showing bar code information can be output
from the Export screen.

Solution Usage Information Report

You can output this report by touching Solution Usage
Status on the Export Data screen.
The report shows, among the bar code data of the
currently used solutions and kits, only the lot numbers
and the expiration dates of unopened and opened
solutions/kits.
The Exp. (Use) field of the report shows the practical
expiration date (unopened or opened, whichever comes
first). Also, the date the bar code was read is shown as
the Date Opened.
The report shows the expiration dates of individual kit
solutions separately.
In addition to the bar code of each station, the bar code
of each kit is also shown.
[Example]
------------------------------ Solution Usage List------------------------------
Exported Date: 2017-03-08 13:06:37
Configuration Name: HE_kit

Station Solution Count Set Management Updated date

1 Xylene -- -- -- --
2 Xylene -- -- -- --
3 Xylene -- -- -- --
4 HCl Alcoholic -- -- -- --
5 /Hematoxylin 2 20 Runs 2017-03-08 13:05:20
6 (W2) Wash Station -- -- -- --
7 (W1) Wash Station -- -- -- --
8 (D1) Drying Station -- -- -- --
9 Alcohol 95% 1 10 Days 2017-03-08 13:05:20
10 Alcohol 100% 2 10 Days 2017-03-08 13:05:20
11 Alcohol 100% -- -- -- --
12 Distilled Water -- -- -- --
13 /Eosin -- -- -- --
14 Distilled Water -- -- -- --
15 Alcohol 95% -- -- -- --
16 (D2) Drying Station -- -- -- --
17 Xylene -- -- -- --
18 Xylene -- -- -- --
19 Xylene -- -- -- --
20 Alcohol 100% -- -- -- --
21 Alcohol 100% -- -- -- --
22 Alcohol 100% -- -- -- --
23 Alcohol 95% -- -- -- --
24(H1) -- -- -- -- --
25(H2) -- -- -- -- --
26(E1-1) End Station1 -- -- -- --
27(E1-2) End Station1 -- -- -- --
28(E1-3) End Station1 -- -- -- --
29(S3) Start Station -- -- -- --
30(S2) Start Station -- -- -- --
31(S1) Start Station -- -- -- --

----------------------- Sakura solution information----------------------

Station Lot Number Exp.(Unopened) Exp. Time Date Opened Exp.(Use)
5 123456 2017-12-13 14 2017-03-08 2017-03-21
13 123456 2017-12-13 14 2017-03-08 2017-03-21
----------------------------- Kit information -----------------------------
H&Ekit250
Lot Number Exp.(Unopened) Exp. Time Date Opened Exp.(Use)
123456 2017-12-13 14 2017-03-08 2017-03-21

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 How to Use the Bar Code Reader, and It's Function (Options)
Process Report
You can output this report by touching Process Report on the Export screen. The report shows, among
the bar code data of the currently used solutions and kits, only the lot numbers and the expiration dates of
unopened and opened solutions/kits.
The Exp. (Use) field of the report shows the practical expiration date (unopened or opened, whichever
comes first). Also, the date the bar code was read is shown as the Date Opened. The report shows the
expiration dates of individual kit solutions separately. The solution usage status at the end of processing is
shown. In addition to the bar code of each station, the bar code of each kit is also shown.
The Usage field shows the solution usage status. In the case of a group, the stations actually used, and
the usage status of each station used, are shown.

[Example]
---------------------------Process Report --------------------------
Exported Date: 2017-03-08 1:07:06 PM
Start:2017-03-08 11:27:34
End :2017-03-08 12:06:33

Program Name: HE_kit

Specimen No : 2

Step Station Solution Time Result Delay Mix Usage

1 S* Start Station --:--:-- 00:00:14
2 1 Xylene 00:05:00 00:05:00 ** ON
3 2 Xylene 00:04:00 00:04:00 ** ON
4 3 Xylene 00:03:00 00:03:00 ** ON
5 11 Alcohol 100% 00:01:00 00:01:00 ** ON
6 10 Alcohol 100% 00:01:00 00:01:00 ** ON
7 9 Alcohol 95% 00:01:00 00:01:00 ** ON
8 W* Wash Station 00:01:00 00:01:00 ** ON
9 5 /Hematoxylin 00:05:00 00:05:00 == ON Runs
11/20
10 W* Wash Station 00:05:00 00:05:00 20% EW
11 13 /Eosin 00:05:00 00:05:00 == ON
12 14 Distilled Water 00:00:10 00:00:10 == ON
13 15 Alcohol 95% 00:00:10 00:00:10 == ON
Days 1/10
14 23 Alcohol 95% 00:00:10 00:00:10 == ON
Days 2/10
15 22 Alcohol 100% 00:01:00 00:01:00 20% ON
16 21 Alcohol 100% 00:01:00 00:01:00 20% ON
17 20 Alcohol 100% 00:01:00 00:01:00 ** ON
18 19 Xylene 00:01:00 00:01:00 ** ON
19 18 Xylene 00:01:00 00:01:00 ** ON
20 17 Xylene 00:01:00 00:01:00 ** ON
21 E1-* End Station 1 --:--:-- 00:58:53

-------------------------- Sakura solution information ----------------------

Station Lot Number Exp.(Unopened) Exp. Time Date Opened Exp.(Use)
5 123456 2017-12-13 14 2017-03-08 2017-03-21
6 123456 2017-12-25 14 2017-01-05 2017-01-18
13 123456 2017-12-13 14 2017-03-08 2017-03-21
14 123456 2017-12-13 14 2017-01-05 2017-01-18
----------------------------- Kit information -----------------------------
H&Ekit250
Lot Number Exp.(Unopened) Exp. Time Date Opened Exp.(Use)
123456 2017-12-13 14 2017-03-08 2017-03-21

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 How to Use the Bar Code Reader, and It's Function (Options)
Solution Configuration Report
You can output this report by touching Solution Configuration List on the Export Data screen.
The solution configuration report also shows the staining kit name.

[Example]
--------------------- Solution Configuration List -------------------
Exported Date: 2017-03-08 13:06:46
Created Date : 2017-01-27 10:40:34
Revised Date : 2017-03-08 10:37:23

Configuration
Name:HE_kit Kit
Name:H&Ekit250
Station Solution Set Management
1 Xylene -- --
2 Xylene 10 Runs
3 Xylene 10 Runs
4 HCl Alcoholic -- --
5 /Hematoxylin -- --
6 (W2) Wash Station -- --
7 (W1) Wash Station -- --
8 (D1) Drying Station -- --
9 Alcohol 95% -- -
10 Alcohol 100% -- --
11 Alcohol 100% -- --
12 Distilled Water -- --
13 /Eosin -- --
14 Distilled Water -- --
15 Alcohol 95% -- --
16(D2) Drying Station -- --
17 Xylene -- --
18 Xylene -- --
19 Xylene -- --
20 Alcohol 100% -- --
21 Alcohol 100% -- --
22 Alcohol 100% -- --
23 Alcohol 95% -- --
24(H1) -- -- --
25(H2) -- -- --
26(E1-1) End Station 1 -- --
27(E1-2) End Station 1 -- --
28(E1-3) End Station 1 -- --
29(S3) Start Station -- --
30(S2) Start Station -- --
31(S1) Start Station -- --
------------------------ Color Information --------------------------
G1 Green
G2 Cream
G3 Yellow
G4 Pink
G5 Magenta
------------------------- Station Parameters ------------------------
Mix Amplitude 30 mm
Mix Frequency 5
Mix Speed 3
Enhanced Wash Frequency 5
Enhanced Wash Speed 3
Basket Lifting Speed 1
Water Wash Cycle Time 60 seconds
Drying Station Temperature 65 C.
Heating Station(1) Temperature 60 C.
Heating Station(2) Temperature 60 C.
Heat up Heating Stations Yes

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 How to Use the Bar Code Reader, and It's Function (Options)
Barcode History Report
You can output this report by touching Barcode History on the Export Data screen.
The barcode history shows the GTIN of each barcode that has been read, lot number, and the date the
barcode was read, for up to 1,000 most recently read barcodes.

[Example]
Barcode History report
--------------------------- Barcode History --------------------------
Exported Date: 2015-06-27 16:47:23

GTIN Lot Number Date Opened

04560133241122 316510 2015-06-19
04560133241105 216513 2015-06-19
04560133241105 216514 2015-06-19
04560133241207 116511 2015-06-19
04560133241115 516215 2015-06-19

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 Deleting Data
Deleting a Solution Configuration/Staining Program/Solution Name/Process Report
This section explains the procedure to delete each item (solution configuration/staining program/solution
name/process report).

Deleting a Solution Configuration

1. Touch Menu at the bottom right of the Stain Process Monitor screen, and then select Delete Data in
the Edit Menu.
2. When the Delete Data screen appears, select Solution Configuration.
3. Touching the key displays the Deletion Procedure window. Under Solution Configuration List, select the
solution configuration you want to delete. This adds a checkmark "" to the Check field. You can select
and delete multiple solution configurations at once. You can also touch All Data to select all solution
configurations in the list. Only solution configurations not used by the available staining programs are
shown under Solution Configuration List. If the solution configuration you want to delete is not in the
list, delete all staining programs that use the applicable solution configuration and then perform this
operation again.
4. Once the target item has been selected, touch Delete. When the Confirmation screen appears, touch
Yes to complete the deletion.

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 Deleting Data
Deleting a Staining Program
1. Touch Menu at the bottom right of the Stain Process
Monitor screen, and then select Delete Data in the
Edit Menu.
2. When the Delete Data screen appears, select
Program.
3. Touching the key displays the Deletion Procedure
window. Under Program List, select the staining
program you want to delete. This adds a checkmark
"" to the Check field. You can select and delete
multiple staining programs at once. You can also
touch All Data to select all solution configurations in
the list.
4. Once the target item has been selected, touch
Delete. When the Confirmation screen appears,
touch Yes to complete the deletion.

Deleting a Solution Name

1. Touch Menu at the bottom right of the Stain Process
Monitor screen, and then select Delete Data in the
Edit Menu.
2. When the Delete Data screen appears, select
Solution Name.
3. Touching the key displays the Deletion Procedure
window. Under Solution List, select the solution name
you want to delete. This adds a checkmark "" to the
Check field. You can select and delete multiple
solution names at once. You can also touch All Data
to select all solution configurations in the list. Only
solutions not used by the available staining programs
are shown under Solution List. If the solution name
you want to delete is not in the list, delete all staining
programs that use the applicable solution and then
perform this operation again.
4. Once the target item has been selected, touch
Delete. When the Confirmation screen appears,
touch Yes to complete the deletion.

Deleting a Process Report

1. Touch Menu at the bottom right of the Stain Process
Monitor screen, and then select Delete Data in the
Edit Menu.
2. When the Delete Data screen appears, select
Process Report.
3. Once the target item has been selected, touch
Delete. When the Confirmation screen appears,
touch Yes to complete the deletion.

Up to 50 process reports can be saved. If the

51st process report is saved, the oldest report
will be automatically deleted to make room for
the latest report.

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 Action to Be Taken upon Power Outage
Power Recovery Operation
This section explains the operation to be performed if a power outage occurred during the stain process.
Once the power is restored, perform the power recovery operation explained below.

1. Once the power is restored, an error buzzer sounds and the Power Outage Alarm screen or Arm Error
window appears on the control panel display to inform the operator that a power outage has occurred.

2. The operator must evacuate the arm to the initial position according to the onscreen instruction. If any
basket is currently set on the arm, remove the basket from the arm according to the onscreen
instruction and then place it in the station shown in brown.
3. To resume stain process, touch the Resume key. Because of the power outage, the staining may not
end by the initially scheduled end time. In this case, the specified time may be delayed by up to the
period after the power outage occurred until the Resume key was touched.
4. To abort stain process, touch the Abort key. Refer to P. 2-11 for the method to abort staining.

A prolonged power outage may cause the tissues to dry up or result in staining problems. If you
know that a power outage will occur, do not use the instrument. If a prolonged power outage has
occurred due to an unexpected problem, take an appropriate action to prevent the tissues from
drying up, and then manually perform the remainder of the staining process.

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 Action to Be Taken upon Error
Error Handling
During operation, the instrument may stop after displaying an error. This section explains the actions to
be taken when errors occur. Most errors accompany a number. Some errors temporarily stop the series
of operations such as staining runs, while other errors display a caution/confirmation window prompting
the operator to check the applicable items. Representative error/caution/confirmation screens are shown
below.

Errors with a Number

Most errors accompany a number and are displayed as follows. You can check the action to be taken
against each error by referring to the section corresponding to the applicable number in Chapter 4,
"Troubleshooting." If further assistance is needed regarding your particular problem, if the problem
persists after taking the specified action, or if the specific problem you have encountered is not listed,
contact the Sakura Finetek Technical Support representative or local representative.

Other Errors
If the error does not accompany a number, follow the onscreen instruction shown in the error window.

In the event of an unexpected problem for which you cannot find any solution,
immediately turn off the power. Next, open the cover, remove the tissues, and protect
them so as not to let them dry up. Then, contact the Sakura Finetek Technical Support
representative or local representative.

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 Chapter 3
Maintenance and Inspection

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 Daily Inspection and Cleaning
Inspection and Cleaning Methods
Inspect and clean the instrument periodically to prevent instrument malfunction and failure. Unless
otherwise specified in this manual, perform inspection and cleaning with the power turned off.

Cleaning the Solution Reservoirs and Baskets

Clean solution reservoirs and baskets with water or neutral detergent. If solution has attached to a
reservoir/basket and the color or stain does not come off easily, soak the reservoir/basket in a commercial
chemical dye cleaner or bleach (diluted to the manufacturer’s instruction), and then clean with neutral
detergent. Sometimes cleaning does not fully remove built-up stains. To prevent stains from building up, it
is recommended that the solution reservoirs/baskets be cleaned after each staining. Alternatively, use
different solution reservoirs/baskets for each solution so that the reservoirs/baskets with solution stains
can still be used.

Cleaning the Drying Stations

In the instrument, the two rightmost stations located at the back are drying stations. First, remove the two
special attachments from the drying stations. Next, clean the stations using neutral detergent, and then
wipe down with a dry cloth. Store the stations by following the removal steps in the reverse order. If the
drying stations are not set properly, the drying function will not operate properly.

Attachment Paraffin tray

Cleaning the Heating Stations

In the instrument, the two rightmost stations located at
the front are heating stations. Remove the heating
stations by holding the two tongues at the edges of the
heating station. Exercise due caution when handling the
heating stations, because these stations remain very hot
immediately after any operation in which the heating
stations have been used. To clean the heating stations,
clean with neutral detergent and then wipe down with a
dry cloth. After cleaning, store the stations in the original
positions. The interior of the heating stations is coated
with Teflon. When cleaning, make sure the coating is not
rubbed with sharp edges, as the coating will be scraped
off.
Special heating station reservoir

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 Daily Inspection and Cleaning

Replacing Activated Carbon Filters

When to replace the activated carbon filters can be easily determined by setting a time limit using the
fume filter management function (refer to P. 1-41 for details). Always replace the two activated carbon
filters in each filter pair together. If the above is not followed, the overall performance of the activated
carbon filters may significantly decrease. To replace the activated carbon filters, find a black knob located
in the filter storage area. Tilt down the knob while pulling it toward you to draw out the filter storage door.
After the filters have been replaced, store the filter storage door into the original position. Push in the door
until a click sound is heard. If the filter storage door is not set properly, the storage door may contact the
arm and cause problems.

Activated carbon filter Activated carbon filter

* One filter is set on the right and Open/close knob
left, respectively.

Cleaning the Arm Holder and Basket Adapter

The arm holder groove and the metal part of the basket adapter become dirty due to repeated staining
operations. Use a cloth, etc., moistened with alcohol to clean the arm holder and basket adapter. Clean
them at least once a month.

Basket adapter
metal part

Arm holder groove

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 Daily Inspection and Cleaning
Cleaning the Water Supply Strainer (Excluding Prisama-P-ED, ES)
Close the main water valve. Open the water supply strainer door at the bottom right of the system.
Opening the cover makes opening the door easier. Use a wrench of 17 mm across flats to turn the lid
counterclockwise and remove the lid. Water in the pipe will come out, so use a container, etc., to catch the
water. Wash the strainer with water using a brush, etc. After washing the strainer, dry and assemble the
strainer, and screw in the lid. Open the water supply plug and confirm that no water leaks from the
strainer. Clean the strainer once a month or so. After the cleaning, perform a water supply test by referring
to
P. 1-42.

Water supply strainer lid Water supply strainer mesh

Cleaning the Cabinet

Clean the entire storage space for the system and solution stations as well as the sink and pans at least
once a month. Remove all trays from the cabinet and clean them using a cloth moistened with water. Also
clean the trays with neutral detergent, and then wipe down with a dry cloth.

In addition to the interior of the instrument, also clean the cover, door and around the exhaust port
regularly. At this time, be sure to turn off the power and disconnect the power plug from the power
outlet. Failure to do so may result in electric shock.

<Corrosion Protection>
The Tissue-Tek Prisma uses corrosion-resistant stainless steel, but the stainless steel may rust depending
on a combination of factors such as the environment (water, air), staining method (type of solution,
concentration), use method (handling of solvent, maintenance), etc. Take note of the following points to
protect the instrument from corrosion.
Keep the solution reservoir lids closed except during the staining process. Remove those solution
reservoirs containing highly corrosive solutions, from the trays, and keep the solutions outside the
instrument. If a highly corrosive solution spilled in the instrument, wipe it off immediately.
Remove all solution trays (including the solution reservoirs) from the instrument once a week or so, wipe
down the trays using a cloth moistened with water, clean them with neutral detergent, and wipe down with
a dry cloth.

Corrosive solutions
Acids [hydrochloric acid (HCL), hydrobromic acid (Hbr), hydrofluoric acid (HF), etc.] and staining
solutions containing an acid
Reagent solutions [Schiff’s reagent, cold Schiff, sulfurous acid solution, resorcin‐fuchsin staining
solution, etc.]
Oxidizing chlorides [ferric chloride (FeCl3), sodium tiosulfate (Na22O3), organic acid (HCOOH), acetic
acid (CH3COOH), bromic acid (C204H2), lactic acid (CH3CH(OH)COOH), etc.)]

If this is not performed, the metal parts may corrode (rust, etc.) quickly. Contact the Sakura Finetek
Technical Support representative or local representative for use of metal cleaners.

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 Daily Inspection and Cleaning
Cleaning the Wash Station Nozzle
Remove the wash station nozzle and clean each part using neutral detergent. Since this is a wash station,
the nozzle need not be wiped down. However, preform a water supply test after installing the nozzle and
setting the solution reservoir, to confirm that the wash station nozzle has been connected correctly.
Refer to P. 1-13 for the installation/removal of the wash station nozzle, or P. 1-42 for the water supply test.

Cleaning the Drain Hose

If the drain hose at the back of the stainer is clogged, staining may be affected and unwanted problems
may result. To prevent such problems, clean the drain hose at least once a month. Remove the drain hose
from the drain elbow and clean the inside of the hose using tap water. If the hose is still clogged after the
cleaning or is cracked and leaking water, etc., replace the hose with a new one. Refer to P. 1-10 for the
installation method of the drain hose.

If you have any question regarding cleaning, contact the Sakura Finetek Technical Support representative
or local representative.

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 Chapter 4
Troubleshooting

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 Troubleshooting
Error Codes Displayed during System Operation
The troubleshooting chart below lists problems that may occur when operating the system. Each
problem description is accompanied by possible causes and actions to be taken. If you have
encountered a problem, use this chart to determine the instrument condition and take an appropriate
action. If the problem persists after taking the specified action, or if the specific problem you have
encountered is not listed in the chart, contact the Sakura Finetek Technical Support representative or
local representative. The number in the table indicates the corresponding error number.

* In the event of an unexpected problem for which you cannot find any solution, immediately
turn off the power. Then, contact the Sakura Finetek Technical Support representative or
local representative.
Error
number Name Cause Action
1 System Error The staining program does not end 30 seconds after the Contact the Sakura Finetek
scheduled time. Technical Support representative
or local representative.
2 Power Outage A power outage has occurred due to abnormal power Contact the Sakura Finetek
supply from the facility. Technical Support representative
or local representative.
The power switch was turned off during the staining Turn on the power switch.
process.
The power cord came off from the power outlet during the Connect the power cord.
staining process.
A power outage signal was sent to the instrument from the This is normal when the facility
UPS. power was cut off. Wait for the
power to be restored.
3 Power Outage Faulty memory on the CPU board of the control panel. Contact the Sakura Finetek
Recovery Failure The voltage of the battery unit installed in the memory of Technical Support representative
the CPU board of the control panel dropped. or local representative.
10 Cover Open The operator opened the cover during staining. Contact the Sakura Finetek
The cover deformed and created a gap between the Technical Support representative
detection sensor and the detection plate during the staining or local representative.
process.
The instrument malfunctioned during the staining process
due to a cover open/close sensor error.
The instrument malfunctioned during the staining process
due to a controller board input error.
The control panel connection harness connecting the CPU
board of the control panel and the controller board was
disconnected during the staining process.
The instrument malfunctioned during the staining process
due to an input error of the CPU board of the control panel.
The instrument malfunctioned during the staining process
due to a CPU error of the CPU board of the control panel.
11 Cover Open (when The operator opened the cover while the arm was Close the cover and resume the
not staining) operating other than during the staining process. process.
The cover deformed and created a gap between the Contact the Sakura Finetek
detection sensor and the detection plate while the arm was Technical Support representative
operating other than during the staining process. or local representative.
The instrument malfunctioned due to a cover open/close
sensor error while the arm was operating other than during
the staining process.
The instrument malfunctioned due to a controller board
input error while the arm was operating other than during
the staining process.
The control panel connection harness connecting the CPU
board of the control panel and the controller board shorted
while the arm was operating other than during the staining
process
The instrument malfunctioned due to an input error of the
CPU board of the control panel while the arm was
operating other than during the staining process.
The instrument malfunctioned due to a CPU error of the
CPU board of the control panel while the arm was
operating other than during the staining process.
20 Processing Halt The operator paused the instrument during the staining Resume the process.
process.

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 Troubleshooting
Error
number Name Cause Action
21 Door Open The operator opened a door while the arm was removing Close the door and resume the
a basket from a station in the frontmost row during the process.
staining process.
The operator opened a door while the arm was storing a Close the door and resume the
basket into a station in the frontmost row during the process.
staining process.
An incompletely latched door suddenly opened during Contact the Sakura Finetek
the staining process. Technical Support representative
The instrument malfunctioned during the staining process or local representative.
due to a door open/close sensor error.
The instrument malfunctioned during the staining process
due to a controller board input error.
The control panel connection harness connecting the
CPU board of the control panel and the controller board
shorted during the staining process.
The instrument malfunctioned during the staining process
due to an input error of the CPU board of the control
panel.
The instrument malfunctioned during the staining process
due to a CPU error of the CPU board of the control
panel.
45 Bar Code The bar code reader or connection cable is faulty. Contact the Sakura Finetek
Reader Circuit Technical Support representative
The external I/F board is faulty.
Overcurrent or local representative.
The controller board is faulty.
The external I/F connection harness is faulty.
50 Exhaust Fan The exhaust fan stopped due to an error of the fan. Contact the Sakura Finetek
Stop Due to an error of the exhaust fan, the fan stop detection Technical Support representative
signal was output even when the fan was turning. or local representative.
Due to a sensor input error of the controller board, the
fan stop detection signal was not transmitted.
The wiring exhaust fan relay harness experienced a
contact failure/disconnection.
70 Battery Voltage The battery unit reached its life. Contact the Sakura Finetek
Low Technical Support representative
or local representative.
80 Motor Unit The control panel connection harness was disconnected. Contact the Sakura Finetek
Communication The CPU board of the control panel is faulty. Technical Support representative
Error The controller board is faulty. or local representative.
81 I/O Unit The control panel connection harness was disconnected. Contact the Sakura Finetek
Communication The CPU board of the control panel is faulty. Technical Support representative
Error The controller board is faulty. or local representative.
90 Vacuum Water leaked and caused the float switch to operate. Contact the Sakura Finetek
Breaker Error The float switch malfunctioned. Technical Support representative
The switch malfunctioned due to a sensor input error of or local representative.
the controller board.
100 Robotic Arm Deviation of each axis Contact the Sakura Finetek
Error Program error Technical Support representative
or local representative.
111 Home Position The X-axis slow-down sensor is faulty. Contact the Sakura Finetek
Sensor Error The X-axis home sensor is faulty. Technical Support representative
(X axis) The controller board is faulty. or local representative.
112 Wire Slack The X-axis wire assembly is loose. Contact the Sakura Finetek
(X axis) Technical Support representative
or local representative.
114 Deviation Error Movement of the X-axis is inhibited by an obstacle or Contact the Sakura Finetek
(X axis) faulty wire drive. Technical Support representative
The X-axis home sensor is faulty. or local representative.
The X-axis encoder is faulty.
The X-axis motor is faulty.
The controller board is faulty.
115 Wire Cut Off The X-axis wire broke or came off from the pulley, etc. Contact the Sakura Finetek
(X axis) The X-axis wire breakage sensor is faulty. Technical Support representative
The controller board is faulty. or local representative.
211 Home Position The Y-axis slow-down sensor is faulty. Contact the Sakura Finetek
Sensor Error The Y-axis home sensor is faulty. Technical Support representative
(Y axis) The controller board is faulty. or local representative.
212 Wire Slack The Y-axis wire assembly is loose. Contact the Sakura Finetek
(Y axis) Technical Support representative
or local representative.

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 Troubleshooting

Error
number Name Cause Action
214 Deviation Error Movement of the Y-axis is inhibited by an obstacle or faulty Contact the Sakura Finetek
(Y axis) wire drive. Technical Support representative
The Y-axis home sensor is faulty. or local representative.
The Y-axis encoder is faulty.
The Y-axis motor is faulty.
The controller board is faulty.
215 Wire Cut Off (Y axis) The Y-axis wire broke or came off from the pulley, etc. Contact the Sakura Finetek
The Y-axis wire breakage sensor is faulty. Technical Support representative
The controller board is faulty. or local representative.
311 Home Position Sensor The Z-axis home sensor is faulty. Contact the Sakura Finetek
Error The controller board is faulty. Technical Support representative
(Z axis) or local representative.
312 Wire slack (Z axis) The Z-axis wire assembly is loose. Contact the Sakura Finetek
Technical Support representative
or local representative.
314 Deviation Error Movement of the Z-axis is inhibited by an obstacle or Contact the Sakura Finetek
(Z axis) faulty wire drive. Technical Support representative
The Z-axis home sensor is faulty. or local representative.
The Z-axis encoder is faulty.
The Z-axis motor is faulty.
The controller board is faulty.
315 Wire Cut Off The Z-axis wire broke or came off from the pulley, etc. Contact the Sakura Finetek
(Z axis) The Z-axis wire breakage sensor is faulty. Technical Support representative
The controller board is faulty. or local representative.
430 Sensor Opened An abnormal value was input to the signal amplification Contact the Sakura Finetek
(Drying Station 1) part of the controller board due to a disconnected Technical Support representative
temperature sensor. or local representative.
The drying station connection harness connecting the
temperature controller board and the controller board was
disconnected.
A disconnected harness was recognized by CPU1 due to
a signal amplification error of the controller board.
Normal judgment was disabled due to a CPU1 error of the
controller board.
431 Sensor Shorted An abnormal value was input to the signal amplification Contact the Sakura Finetek
(Drying Station 1) part of the controller board due to a shorted temperature Technical Support representative
sensor. or local representative.
The drying station connection harness connecting the
temperature controller board and the controller board
shorted.
A shorted harness was recognized by CPU1 due to a
signal amplification error of the controller board.
Normal judgment was disabled due to a CPU1 error of the
controller board.
432 Temperature Low Power could not be supplied to the heater due to a Contact the Sakura Finetek
(Drying Station 1) disconnection or connection failure of the heater. Technical Support representative
Power could not be supplied to the heater due to a SSR or local representative.
error of the temperature controller board.
Power could not be supplied to the drying heater due to a
disconnection of the drying station connection harness
connecting the temperature controller board and the
controller board.
Power could not be supplied to the heater due to a CPU1
error of the controller board.
The heater voltage selector switch is set incorrectly.
The power-supply voltage selector switch is set incorrectly.
433 Thermal Fuse (Drying The SSR3 on the temperature controller board Contact the Sakura Finetek
Station 1) experienced a short-circuit failure. Technical Support representative
The heater remained on due to a driver error of the or local representative.
controller board.
A false detection occurred due to an error of the SSR1 for
power-supply voltage detection.
The drying station connection harness connecting the
temperature controller board and the controller board was
disconnected.
The instrument malfunctioned due to an over-temperature
protection input error of the controller board.
The heater voltage selector switch is set incorrectly.
The power-supply voltage selector switch is set incorrectly.

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 Troubleshooting
Error
number Name Cause Action
434 Fan Stop The fan is faulty. Contact the Sakura
(Drying Station 1) An operation signal was not output due to a controller board Finetek Technical
error. Support representative or
The connection cable connecting the temperature controller local representative.
board and the controller board was disconnected.
The fan could not be driven due to a driver error of the
temperature controller board.
The connection cable connecting the temperature controller
board and the controller board was disconnected.
A stopped fan was recognized by CPU1 due to a signal input
error of the controller board.
Normal judgment was disabled due to a CPU1 error of the
controller board.
530 Sensor Opened An abnormal value was input to the signal amplification part of Contact the Sakura
(Drying Station 2) the controller board due to a disconnected temperature sensor. Finetek Technical
The drying station connection harness connecting the Support representative or
temperature controller board and the controller board was local representative.
disconnected.
A disconnected harness was recognized by CPU1 due to a
signal amplification error of the controller board.
Normal judgment was disabled due to a CPU1 error of the
controller board.
531 Sensor Shorted An abnormal value was input to the signal amplification part of Contact the Sakura
(Drying Station 2) the controller board due to a shorted temperature sensor. Finetek Technical
The drying station connection harness connecting the Support representative or
temperature controller board and the controller board shorted. local representative.
A shorted harness was recognized by CPU1 due to a signal
amplification error of the controller board.
Normal judgment was disabled due to a CPU1 error of the
controller board.
532 Temperature Low Power could not be supplied to the heater due to a disconnection Contact the Sakura
(Drying Station 2) or connection failure of the heater. Finetek Technical
Power could not be supplied to the heater due to a SSR error of Support representative or
the temperature controller board. local representative.
Power could not be supplied to the drying heater due to a
disconnection of the drying station connection harness
connecting the temperature controller board and the controller
board.
Power could not be supplied to the heater due to a CPU1 error
of the controller board.
The heater voltage selector switch is set incorrectly.
The power-supply voltage selector switch is set incorrectly.
533 Thermal Fuse Short-circuit failure of the SSR4 on the temperature control Contact the Sakura
(Drying Station 2) board Finetek Technical
The heater remained on due to a driver error of the controller Support representative or
board. local representative.
A false detection occurred due to an error of the SSR2 for
power-supply voltage detection.
The drying station connection harness connecting the
temperature controller board and the controller board was
disconnected.
The instrument malfunctioned due to an over-temperature
protection input error of the controller board.
The heater voltage selector switch is set incorrectly.
The power-supply voltage selector switch is set incorrectly.
534 Fan Stop The fan is faulty. Contact the Sakura
(Drying Station 2) An operation signal was not output due to a controller board Finetek Technical
error. Support representative or
The connection cable connecting the temperature controller local representative.
board and the controller board was disconnected.
The fan could not be driven due to a driver error of the
temperature controller board.
The connection cable connecting the temperature controller
board and the controller board was disconnected.
A stopped fan was recognized by CPU1 due to a signal input
error of the controller board.
Normal judgment was disabled due to a CPU1 error of the
controller board.

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 Troubleshooting
Error
number Name Cause Action
630 Sensor Opened An abnormal value was input to the signal amplification part of the Contact the Sakura Finetek
(Heating Station 2) controller board due to a disconnected temperature sensor. Technical Support
The heating station connection harness connecting the temperature representative or local
controller board and the controller board was disconnected. representative.
A disconnected harness was recognized by CPU1 due to a signal
amplification error of the controller board.
Normal judgment was disabled due to a CPU1 error of the controller
board.
631 Sensor Shorted An abnormal value was input to the signal amplification part of the Contact the Sakura Finetek
(Heating Station 1) controller board due to a shorted temperature sensor. Technical Support
The heating station connection harness connecting the temperature representative or local
controller board and the controller board shorted. representative.
A shorted harness was recognized by CPU1 due to a signal
amplification error of the controller board.
Normal judgment was disabled due to a CPU1 error of the controller
board.
632 Temperature Low Power could not be supplied to the heater due to a disconnection or Contact the Sakura Finetek
(Heating Station 1) connection failure of the heater. Technical Support
Power could not be supplied to the heater due to a SSR error of the representative or local
temperature controller board. representative.
Power could not be supplied to the heater due to a disconnection of
the heating station connection harness connecting the temperature
controller board and the controller board.
Power could not be supplied to the heater due to a CPU1 error of
the controller board.
Solution exchange to a cold solution occurred during the operation.
The heater voltage selector switch is set incorrectly.
The power-supply voltage selector switch is set incorrectly.
633 Thermal Fuse (Heating The SSR3 on the temperature controller board experienced a short- Contact the Sakura Finetek
Station 1) circuit failure. Technical Support
The heater remained on due to a driver error of the controller board. representative or local
A false detection occurred due to an error of the SSR1 for power- representative.
supply voltage detection.
The heating station connection harness connecting the temperature
controller board and the controller board was disconnected.
The instrument malfunctioned due to an over-temperature protection
input error of the controller board.
The heater voltage selector switch is set incorrectly.
The power-supply voltage selector switch is set incorrectly.
730 Sensor Opened An abnormal value was input to the signal amplification part of the Contact the Sakura Finetek
(Heating Station 2) controller board due to a disconnected temperature sensor. Technical Support
The heating station connection harness connecting the temperature representative or local
controller board and the controller board was disconnected. representative.
A disconnected harness was recognized by CPU1 due to a signal
amplification error of the controller board.
Normal judgment was disabled due to a CPU1 error of the controller
board.
731 Sensor Shorted An abnormal value was input to the signal amplification part of the Contact the Sakura Finetek
(Heating Station 2) controller board due to a shorted temperature sensor. Technical Support
The heating station connection harness connecting the temperature representative or local
controller board and the controller board shorted. representative.
A shorted harness was recognized by CPU1 due to a signal
amplification error of the controller board.
Normal judgment was disabled due to a CPU1 error of the controller
board.
732 Temperature Low Power could not be supplied to the heater due to a disconnection or Contact the Sakura Finetek
(Heating Station 2) connection failure of the heater. Technical Support
Power could not be supplied to the heater due to a SSR error of the representative or local
temperature controller board. representative.
Power could not be supplied to the heater due to a disconnection of
the heating station connection harness connecting the temperature
controller board and the controller board.
Power could not be supplied to the heater due to a CPU1 error of
the controller board.
Solution exchange to a cold solution occurred during the operation.
The heater voltage selector switch is set incorrectly.
The power-supply voltage selector switch is set incorrectly.

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 Troubleshooting
Error
number Name Cause Action
733 Thermal Fuse The SSR3 on the temperature controller board experienced a Contact the Sakura
(Heating Station 2) short-circuit failure. Finetek Technical
The heater remained on due to a driver error of the controller Support representative or
board. local representative.
A false detection occurred due to an error of the SSR1 for
power-supply voltage detection.
The heating station connection harness connecting the
temperature controller board and the controller board was
disconnected.
The instrument malfunctioned due to an over-temperature
protection input error of the controller board.
The heater voltage selector switch is set incorrectly.
The power-supply voltage selector switch is set incorrectly.
800 Coverslipper Not The RS232C cable between the coverslipper and this instrument Connect the cable
Connected is not connected correctly. correctly.
The coverslipper is not powered up. Turn on the coverslipper
power.
The instrument malfunctioned due to a communication driver Contact the Sakura
error of the controller board. Finetek Technical
The instrument malfunctioned due to an electrical error of the Support representative or
coverslipper. local representative.
802 Coverslipper The cover of this instrument was opened while the start station Close the cover.
Operation Stop of the coverslipper was moving into the instrument.
The cover deformed and created a gap between the cover Contact the Sakura
open/close sensor and the detection plate while the start station Finetek Technical
of the coverslipper was moving into the instrument. Support representative or
The instrument malfunctioned due to a cover open/close sensor local representative.
error while the start station of the coverslipper was moving into
the instrument.
The instrument malfunctioned due to a controller board input
error while the start station of the coverslipper was moving into
the instrument.
The control panel connection harness connecting the CPU board
of the control panel and the controller board shorted while the
start station of the coverslipper was moving into the instrument.
The instrument malfunctioned due to an input error of the CPU
board of the control panel while the start station of the
coverslipper was moving into the instrument.
The instrument malfunctioned due to a CPU error of the CPU
board of the control panel while the start station of the
coverslipper was moving into the instrument.
An error occurred in the coverslipper.
900 File System Error The CF card inserted to CF drive A is not recognized. Contact the Sakura
(Drive: A) Finetek Technical
Support representative or
local representative.
901 File Not Found The operator attempted to export data to a file without inserting a Insert the CF card
(Drive: A) CF card in CF drive A. correctly, and then
perform the operation.
The operator attempted to export data to a file by inserting a CF Contact the Sakura
card in CF drive A in which the maximum number of files have Finetek Technical
been registered . Support representative or
The operator attempted to export data to a file by inserting in CF local representative.
drive A a CF card that was formatted by a file system other than
FAT.
902 Space Not Available The CF card inserted to CF drive A is full. Contact the Sakura
(Drive: A) Finetek Technical
Support representative or
local representative.
903 Write Protect Error If data is to be exported to a file, the file to be exported in CF Contact the Sakura
(Drive: A) drive A has a write-protected file whose name is exactly same as Finetek Technical
the file exported to CF drive A. Support representative or
local representative.
905 File System Error The control file required for instrument operation is not found in Contact the Sakura
(Drive: B) the CF card set in CF drive B. Finetek Technical
Support representative or
local representative.
906 File Not Found The control file required for system operation is corrupted. Contact the Sakura
(Drive: B) The CF card inserted to CF drive B is full. Finetek Technical
The control file required for instrument operation is write- Support representative or
protected. local representative.

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 Troubleshooting
Instrument Conditions and Recovery Actions
The troubleshooting chart below lists problems that may occur during system operation. Probable causes
and recovery actions are described for each problem. Use this information to identify the instrument
condition and resolve the problem. If the problem persists after taking the specified action, or if the specific
problem you have encountered is not listed in the chart, contact the Sakura Finetek Technical Support
representative or local representative.

* In the event of an unexpected problem for which you cannot find any solution, immediately turn
off the power. Then, contact the Sakura Finetek Technical Support representative or local
representative.

Instrument condition Check Action

The power is not supplied even Power is not supplied. Check that the power supply condition
when the power switch is turned on. on the facility side is normal. Check the
power cord for abnormality.
The power indicator on the display The circuit protector has tripped. Contact the Sakura Finetek Technical
does not illuminate. The power input part is abnormal. Support representative or local
The switching power supply is abnormal. representative.
The control panel LED board is faulty.
When the power switch is turned on, Mis-match of the trip current of the circuit Contact the Sakura Finetek Technical
the built-in circuit protector in the protector. Support representative or local
power switch or the circuit protector The power-supply voltage selector switch or representative.
installed in the control box trips. heater voltage selector switch is set
incorrectly.
Short-circuited switching power input part.
Short-circuited drying station drive part.
Short-circuited heating station drive part.
When a metal part of the instrument Insulation failure occurred when the Contact the Sakura Finetek Technical
is touched, tingling sensation is felt. enclosure was not grounded, and the leak Support representative or local
current flowed through the operator’s body. representative.
When the power is turned on, the Power is not supplied to the control panel. Contact the Sakura Finetek Technical
screen remains dark. The CPU board of the control panel is faulty. Support representative or local
The LED backlight cable is faulty. representative.
The LED drive circuit unit is faulty.
The LCD turns on but the screen The LCD FPC2 on the control panel is not Contact the Sakura Finetek Technical
remains while when the power is connected all the way. Support representative or local
turned on. The CPU board of the control panel is faulty. representative.
The LCD is faulty.
The program does not load after the The program file is corrupted. Contact the Sakura Finetek Technical
power is turned ON. The CPU board of the control panel is faulty. Support representative or local
representative.
The clock displayed at the top of the The battery unit is faulty. Contact the Sakura Finetek Technical
screen no longer keeps the correct The CPU board of the control panel is faulty. Support representative or local
time. representative.
When an effective button is touched Calibration error of the touch panel detection Contact the Sakura Finetek Technical
on the control panel screen, the position. Support representative or local
button does not react. Or, the input The CPU board of the control panel is faulty. representative.
position is off. The LCD is faulty.
The LCD became much darker than Effect of environmental temperature when the After the power is on, wait for a while,
before. power was turned on. and the display should become brighter.
The LED drive circuit unit is faulty. Contact the Sakura Finetek Technical
The LED blacklight is faulty (deteriorated). Support representative or local
representative.
The LCD suddenly flickers or colors The LCD FPC2 on the control panel is not Contact the Sakura Finetek Technical
become misaligned. This does not connected properly. Support representative or local
include a momentary freezing of the The CPU board of the control panel is faulty. representative.
display when the screen is switched The LCD is faulty.
during a process.
The buzzer at the back of the control Program setting. Check the setting of the key input sound
panel does not sound. in "System Setup."
The speaker is faulty. Contact the Sakura Finetek Technical
The CPU board of the control panel is faulty. Support representative or local
representative.
The control panel supported by the Foreign matter is stuck between the control Remove the foreign matter.
DP arm of the instrument does not panel and the DP arm.
operate smoothly and cannot be The pin (washer) regulating the rotation Contact the Sakura Finetek Technical
moved to an easy-to-operate range of the rotation mechanism broke and Support representative or local
position. got bitten by the rotation part. representative.
The control panel supported by the The tightening nut or hex socket head bolt of Contact the Sakura Finetek Technical
DP arm of the instrument can no the rotation part is loose. Support representative or local
longer be fixed at an easy-to-operate The friction plate or swing mechanism collar representative.
position. used at the rotation part has worn.

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 Troubleshooting

4-8

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 Troubleshooting
Instrument Condition Check Action
A special staining tray can The detection pin no longer moves. Contact the Sakura Finetek Technical Support
no longer be recognized. False detection occurred due to a special staining tray detection representative or local representative.
sensor error.
False detection occurred due to a sensor input error of the
controller board.
False detection occurred due to a CPU1 error of the controller
board.
Detection is still disabled The detection part of the basket adapter deformed and can no Contact the Sakura Finetek Technical Support
after installing a 10-slide longer detect properly. representative or local representative.
basket load adapter at the False detection occurred due to a special staining basket sensor
start station. error.
False detection occurred due to a sensor input error of the
controller board.
False detection occurred due to a CPU1 error of the controller
board.
The transfer arm still The instrument was operated with the stopping of the transfer arm Contact the Sakura Finetek Technical Support
moves after the cover is disabled in the service mode. representative or local representative.
opened. The cover open/close sensor is outputting a detection signal.
False detection occurred due to a cover open/close sensor error.
False detection occurred due to an input error of the controller
board.
The control panel connection harness connecting the CPU board of
the control panel and the controller board shorted.
The false detection occurred due to a CPU board error of the
control panel.
The door open/close The door switch plate installed on the right side of the door has Contact the Sakura Finetek Technical Support
sensor does not detect deformed. representative or local representative.
opening/closing of the The door open/close sensor suffered mechanical damage.
door. False detection occurred due to an electrical error of the door
open/close sensor.
False detection occurred due to an input error of the controller
board.
The control panel connection harness connecting the CPU board of
the control panel and the controller board shorted.
The false detection occurred due to a CPU board error of the
control panel.
Stored instrument setup The control file storing the instrument setup data was lost. Contact the Sakura Finetek Technical Support
data was initialized. The control file storing the instrument setup data is corrupted. representative or local representative.
The saved password The control file storing the password setting information was lost. Contact the Sakura Finetek Technical Support
setting information was The control file storing the password setting information is representative or local representative.
initialized. corrupted.

The saved solution names The control file storing the solution name information was lost. Contact the Sakura Finetek Technical Support
were initialized. The control file storing the solution name information is corrupted. representative or local representative.

The saved solution The control file storing the solution configuration information was Contact the Sakura Finetek Technical Support
configuration was lost. representative or local representative.
initialized. The control file storing the solution configuration information is
corrupted.
The saved staining The control file storing the staining program information was lost. Contact the Sakura Finetek Technical Support
program was initialized. The control file storing the staining program information is representative or local representative.
corrupted.
Sakura solutions or kit The definition file (solution, kit data base) provided by the dealer Contact the Sakura Finetek Technical Support
solutions are not was lost. representative or local representative.
supported. The definition file (solution, kit data base) provided by the dealer is
corrupted.
The arm cannot be moved Foreign matters are attached to the slide shaft of each axis. Contact the Sakura Finetek Technical Support
manually by hand when the The bearing is too tight to move the slide shaft of each axis due to representative or local representative.
power is off. rust, scratches or deformation.
Each axis does not move due to an obstacle present in its moving Remove the obstacle.
direction.
Each axis does not move in the specified direction due to an Contact the Sakura Finetek Technical Support
abnormality of the axis drive wire and its transmission mechanism. representative or local representative.
A basket cannot be placed The solution reservoir is not installed in the correct position. Install the solution reservoir in the correct
in the correct position position.
during operation. The basket adapter has deformed. Contact the Sakura Finetek Technical Support
representative or local representative.
The tray is not installed in the correct position. Install the tray in the correct position.
The home detection mechanism of each axis has not been adjusted Contact the Sakura Finetek Technical Support
correctly. representative or local representative.
A solution reservoir different from the one set in the solution Install a solution reservoir matching the
configuration was installed. solution configuration.
No solution reservoir is installed. Install a solution reservoir matching the
solution configuration.
The staining process was aborted or ended, but the basket has not Remove the basket.
been removed.
The lid of the solution reservoir remains on. Remove the lid of the solution reservoir.
The paraffin tray in the drying station is facing the wrong direction. Install it in the correct direction.

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 Troubleshooting
Instrument Condition Check Action
The basket was placed in The solution reservoir is not installed in the correct position. Install the solution reservoir in the correct
the reservoir, but does not position.
come off from the adapter. The basket adapter has deformed. Contact the Sakura Finetek Technical Support
The home detection mechanism of each axis has not been representative or local representative.
adjusted correctly.
When the facility is The water supply plug on the facility side, or the flow rate Open the flow rate adjustment valve.
operating under the adjustment valve in the instrument, is closed.
conditions within the use Setting of the water flow time under control is too short. Contact the Sakura Finetek Technical Support
environment of the representative or local representative.
instrument, water is not The strainer is clogged. Clean the strainer.
supplied by the water The seat of the check valve is stuck (built-in check valve in the Contact the Sakura Finetek Technical Support
supply operation. pressure relief valve or the check valve before the water supply representative or local representative.
manifold).
The water supply solenoid valve is faulty.
The valve seat of the vacuum breaker is stuck or leaking.
The float switch has actuated (error code: 90).

The instrument power is Contaminants entered the water supply solenoid valve seat. Contact the Sakura Finetek Technical Support
off, but water is leaking The water supply solenoid valve is damaged. representative or local representative.
from the water supply
nozzle.
When the facility was The flow rate adjustment valve is clogged. Use the flow rate adjustment valve to adjust
operating under the the amount of water supplied.
conditions within the use The strainer is clogged. Clean the strainer.
environment of the The O-ring of the water supply nozzle is damaged. Contact the Sakura Finetek Technical Support
instrument, the water level The wash reservoir is damaged. representative or local representative.
reached by supplying The wash reservoir is not installed properly.
water to the instrument is
lower than before.
When the facility was The open angle of the flow rate adjustment valve was changed. Use the flow rate adjustment valve to adjust
operating under the the amount of water supplied.
conditions within the use
environment of the The pressure relief valve is damaged. Contact the Sakura Finetek Technical Support
instrument, the water level representative or local representative.
reached by supplying The water supply capacity on the facility side has changed. Use the flow rate adjustment valve to adjust
water to the instrument is the amount of water supplied.
more than before.

Water is leaking from the A component of the water supply part is damaged. Contact the Sakura Finetek Technical Support
instrument due to a The component connection point at the water supply part is leaking representative or local representative.
problem with water supply water.
or drain. The connection of the drain hose is loose or clogged.
The sink is leaking water.
The solution odor is No fume cartridge is set. Install a fume cartridge.
leaked from the instrument The fume cartridge was set without unpacking it. Unpack the fume cartridge.
when the instrument is The fume cartridge is not installed properly. Install the fume cartridge correctly.
operating. The fume cartridge has reached its life. Replace the fume cartridge.
The exhaust fan stopped. Contact the Sakura Finetek Technical Support
representative or local representative.
The air inlet port in the system was blocked. Check the air inlet port for foreign matters.
Each gasket of the fume control unit deteriorated. Contact the Sakura Finetek Technical Support
representative or local representative.
The exhaust outlet port is blocked. Do not block the exhaust outlet port.
When connecting the exhaust duct, it is not connected correctly. Contact the Sakura Finetek Technical Support
The exhaust duct is too long. representative or local representative.
The exhaust duct was crushed.
The exhaust system on the facility side is not operating. Check the exhaust system on the facility side.
Solution odor leaks from The door is open. Close the door.
the instrument when the The cover is open Close the cover.
operation is stopped with There is no lid on the solution reservoir. Put the lid on the solution reservoir.
solution still in the
instrument.

The cover does not open The cover was pushed open at an angle. Contact the Sakura Finetek Technical Support
smoothly. representative or local representative.
The cover (top plate) is bitten. Check for foreign matters and remove them, if
found.
The rotary damper and rack are bitten or damaged. Check for foreign matters and remove them, if
found.
The roller pack is bitten or damaged. Contact the Sakura Finetek Technical Support
The spring stay is damaged. representative or local representative.
The cover does not close The cover closes at an angle or does not close fully. Contact the Sakura Finetek Technical Support
smoothly or fully. The cover is distorted. representative or local representative.
The cover (top plate) is bitten. Check for foreign matters and remove them, if
found.
The rotary damper and rack are bitten or damaged. Contact the Sakura Finetek Technical Support
The roller pack is bitten or damaged. representative or local representative.
The spring stay is damaged.
The cover stay adjustment is inappropriate.

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 Troubleshooting
Instrument Condition Check Action
The cover has Dirty or solution is attached. Clean the cover if dirty or solution is
deteriorated. attached.
Effect of ultraviolet light. Contact the Sakura Finetek Technical
Support representative or local
representative.
The temperature at the installation location has changed. Use the instrument in an environment free
from temperature difference.
The door does not The oil damper is deformed or damaged. Contact the Sakura Finetek Technical
move smoothly or The stay is deformed or damaged. Support representative or local
close. The door hinge is deformed or damaged. representative.
The door switch plate is deformed or damaged.
The door would not Entry of foreign matter Check for foreign matters and remove
open. them, if found.
The door would not The one-touch open catch requires adjustment or is Contact the Sakura Finetek Technical
close. damaged. Support representative or local
representative.
No error is displayed The [Normal Signal] is not transmitted due to an external Contact the Sakura Finetek Technical
on the screen, but an wiring error. Support representative or local
external alarm is The [Normal Signal] is not output due to an external I/F representative.
output. board relay error.
The [Normal Signal] is not output due to an error in the
controller board driver circuit.
The control panel connection harness is disconnected.
The [Normal Signal] is not output due to a CPU board
error of the control panel.
Rust generated. Corrosive solution was spilled in the sink or instrument. Clean the solution without delay.
The instrument was stopped for a long period of time Put the lids on the solution stations and
with corrosive and volatile solutions still left in the store the solutions outside the instrument.
instrument.

The instrument was stopped for a long period of time Wipe off any water, etc., attached to the
with water still attached to the sink or tray. instrument.

Noises generated by Noise from the gear meshing part at the bottom of the Z- Contact the Sakura Finetek Technical
each drive axis axis motor Support representative or local
became louder than Noise from the gear meshing part at the bottom of the X- representative.
before. axis motor
Noise from the gear meshing part at the bottom of the Y-
axis motor
The solution reservoir, Dropped. Exercise caution when using the
basket or basket instrument.
adapter has An attempt was made to install the instrument under Avoid using the instrument under
deteriorated or unreasonable conditions. unreasonable conditions.
damaged. Effect of solution due to the staining process. Contact the Sakura Finetek Technical
Support representative or local
representative.
Smoke came from the The terminal block of the primary power line became Contact the Sakura Finetek Technical
instrument. loose and the terminals were overheated and smoke Support representative or local
generated from electric wire. representative.
Smoke generated due to an abnormal drying station.
Smoke generated due to an abnormal heating station.
The insulation of the power cable was lost and the leak
current continued to flow, causing abnormal heat
generation.
Solution or water was Human error Contact the Sakura Finetek Technical
spilled inside the Support representative or local
instrument. representative.
The bar code cannot The bar code was read more than 10 seconds after Read the bar code within 10 seconds
be read. touching the Read button. after touching the Read button.
A bar code reader other than the specified product was Use the specified bar code reader.
used.
The bar code reader is faulty. Contact the Sakura Finetek Technical
Power is not supplied to the bar code reader. Support representative or local
The circuit on the instrument side is faulty. representative.
The bar code is in poor state (not printed clearly, dirty,
scratched, etc.)
The bar code pertains to an expired product. Be sure to use an unexpired product.
The product code DB file provided by the dealer is Contact the Sakura Finetek Technical
damaged. Support representative or local
The bar code reader is not set correctly. representative.

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 Troubleshooting

Please visit our website sakura.eu

Sakura Finetek Europe B.V., Flemingweg 10a, 2408 AV Alphen aan den Rijn,
P.O. Box 362, 2400 AJ Alphen aan den Rijn, The Netherlands

Sakura Finetek Japan Co., Ltd.

1-9 Honcho 3-chome Nihombashi Chuo-ku Tokyo, Japan
4-12
M01-019E-04
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