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BHI Broth M210
Intended Use:
BHI Broth is employed for the propagation of fastidious pathogenic cocci and other organisms associated with blood culture
work and allied pathological investigations.
Composition**
Ingredients Gms / Litre
HM infusion powder # 12.500
BHI powder 5.000
Proteose peptone 10.000
Dextrose (Glucose) 2.000
Sodium chloride 5.000
Disodium hydrogen phosphate 2.500
Final pH ( at 25°C) 7.4±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Calf brain infusion from
Directions
Suspend 37.0 grams in 1000 ml purified/distilled water. Dispense into bottles or tubes and sterilize by autoclaving
at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared,
otherwise, it should be boiled or steamed for a few minutes and then cooled before use.
Principle And Interpretation

BHI Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. It is also
used to prepare the inocula for antimicrobial susceptibility testing. BHI Broth is a modification of the original
formulation of Rosenow, where he added pieces of brain tissues to dextrose broth (1). BHI Broth is also the preferred
medium for anaerobic bacteria, yeasts and moulds (2,3,4). This medium is nutritious and well buffered to support the
growth of wide variety of organisms (2,5,6). With the addition of 10% defibrinated sheep blood, it is useful for
isolation and cultivation of Histoplasma capsulatum (7) and other fungi. For selective isolation of fungi,
addition of gentamicin and/or chloramphenicol is recommended (8).
Proteose peptone, HM infusion powder and BHI powder serve as sources of carbon, nitrogen, essential growth
factors, amino acids and vitamins. Dextrose serves as a source of energy. Disodium phosphate helps in maintaining
the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium.
Type of specimen
Clinical samples : pathological samples like faeces; Food samples.
Specimen Collection and Handling

For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines
(4,9,10).
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (11,12).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/
protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens
and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens.
Safety guidelines may be referred in individual safety data sheets.
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
Limitations
1.As organisms differ in their nutritional requirements, some fastidious organisms may be inhibited or may show poor growth.
2. Biochemical and serological tests must be performed for confirmation of isolated organism.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored
at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder.
Colour and Clarity of prepared medium
Light to medium amber coloured, clear solution without any precipitate .
Reaction
Reaction of 3.7% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 24-48 hours.
Organism Inoculum Growth

(CFU)
Neisseria meningitidis ATCC good-luxuriant
50-100
13090
Streptococcus pneumoniae 50-100 good-luxuriant
ATCC 6303
Streptococcus pyogenes 50-100 good-luxuriant
ATCC 19615
Candida albicans ATCC 50-100 good-luxuriant
10231 (00054*)
Staphylococcus aureus 50-100 good-luxuriant
subsp. aureus ATCC
25923 (00034*)
Enterococcus faecalis ATCC 50-100 good-luxuriant
29212 (00087*)
Key : *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation.
Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after
use. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical
sample must be decontaminated and disposed of in accordance with current laboratory techniques (11,12).
Reference
1. Rosenow, 1919, J. Dental Research, 1:205.
2. MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I,
Williams and Wilkins, Baltimore.
3. Atlas R. M., 1993, Handbook of Microbiological Media, 147-153, CRC Press, Boca Raton, FL.
4. Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological
Examination of Foods, 5th Ed., American Public Health Association, Washington, D.C.
5. Roseburg T. et al, 1944, J. Inf. Dis., 74:131
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
8. Conant N. F., 1950, Diagnostic Procedures and Reagents, 3rd Ed., APHA Inc., New York
7.Howard B., Keiser J. F., Weissfeld A. et al, 1994, Clinical and Pathogenic Microbiology, 2nd Ed., Mosby Co.
8.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical
Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C.
9. Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,
APHA Inc.,Washington, D.C.
10.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed.,
Washington D.C.
11.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.
12.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual
of Clinical Microbiology, 11th Edition. Vol. 1.
Revision : 04 / 2022
IVD In vitro diagnostic medical

device
CE Marking
30°C Storage temperature
10°C
Do not use if package is

damaged
HiMedia Laboratories Pvt. Limited,

Plot No. C-40, Road No.21Y,
MIDC, Wagle Industrial Area,
Thane (W)
-400604,MS,India
CE Partner 4U ,Esdoornlaan 13,

EC REP
3951 DB Maarn The Netherlands,
www.cepartner 4u.eu
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Corporate Office : Plot No.C-40, Road No.21Y, MIDC, Wagle Industrial Area, Thane (W) - 400604, India. Customer care No.:
022-6147 1919 Email: [email protected] Website: www.himedialabs.com

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Inserto Himedia

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BHI Broth M210

Principle And Interpretation

Specimen Collection and Handling

Please refer disclaimer Overleaf.

Organism Inoculum Growth

Please refer disclaimer Overleaf.

IVD In vitro diagnostic medical

30°C Storage temperature

Do not use if package is

HiMedia Laboratories Pvt. Limited,

CE Partner 4U ,Esdoornlaan 13,

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