Igcse Biology 4ed TR End of Course Test3 Answers

CAMBRIDGE IGCSE™ BIOLOGY: PRACTICE EXAM-STYLE TEST 3: ALTERNATIVE TO PRACTICAL ANSWERS
Practice exam-style test 3: Alternative to

practical – answers
40 marks
Q Answer Marks
1a Smaller air spaces / shallower spongy mesophyll layer / thinner. [1] To reduce the diffusion distance 2
for carbon dioxide from the stomata to the palisade mesophyll layer. [1]
1bi The indicator would turn purple [1] because the amount of carbon dioxide absorbed by the 2
leaves (for photosynthesis) in bright sunlight exceeds the amount of carbon dioxide released
(by respiration). [1]
1 b ii Any three from: 3

The indicator in the tube containing the leaf from plant 1 would change colour first / take less time
to change colour than the tube containing the leaf of plant 2. [1] This is because the leaf from Plant max.
1 has a greater stomatal density. [1] The rate of photosynthesis / gas exchange of the leaf from
plant 1 is faster [1] which means that diffusion [1] of carbon dioxide from the indicator into the
atmosphere in the tube is faster. [1]
1c Variable 1: Temperature AND 2

Explanation: The rate of diffusion of carbon dioxide from the leaf into the solution would be
affected by temperature (and this investigation should have only one factor that is changed:
stomatal density). [1]
Variable 2: Species of plant / age of the leaf / how long since the leaf was taken from the plant /
health of leaf. AND
Explanation: The stomatal density is likely to be affected by these factors (and this
investigation should have only one factor that has had an impact on stomatal density: carbon
dioxide concentration). [1]
1di Judging colour by eye is qualitative [1] and open to interpretation / subjective. [1] 2
1 d ii This will allow the experimenter to identify any anomalous readings, which can be excluded from 1
further analysis. [1]
1e Role of carbohydrate in plant cells Name of carbohydrate 2

Storage as an insoluble material starch
Forms cell walls cellulose
[1 mark for each row]
2a A percentage increase accounts for the fact that the sizes of the different balls of dough are 2
probably different at the beginning of the investigation, [1] and allows a valid comparison of the
change in height. [1]
2b Measuring cylinder. [1] 1
Cambridge IGCSE™ Biology – Martindill © Cambridge University Press 2021 1

Q Answer Marks
2c Any two from: 2

Mass of dough; [1] temperature of incubation; [1] time of incubation; [1] mass or volume of
ingredients other than flour. [1] max.
2di Iodine (solution). [1] 1
2 d ii Dropping pipette. [1] 1
2 d iii Obtain two samples of flour of equal mass. [1] Add iodine solution of equal volume to both samples. 4
[1] Both will turn blue–black in colour. [1] Determine which colour is the darker, representing the
flour with the greater starch content, using a colour chart/ colorimeter. [1]
3a 1
time / hours pH
Investigation 1 Investigation 2 Investigation 3 Mean
0 7.8 7.8 7.8 7.8
2 5.9 5.8 5.4 5.7
4 7.4 7.2 7.0 7.2
6 9.6 10.7 10.0 10.1
8 20.1 17.0 14.5 17.2
10 11.4 6.4 13.4 10.4
3b Time point = 4 hours. 1
3c Orientation correct: Time / hours on x-axis and pH on the y-axis. [1] 4

Axes fully labelled (with units for time). [1]
Linear scales and at least half of the grid used. [1]
Neat line, ruled to join points, with no extrapolation. [1]
3d Answer will depend on graph sketch. [1 mark for showing the interpolation lines on the graph; 2
1 mark for correct answer]
3e One pair from: 2

Volume of milk / mass of lactose is used up. [1] Substrate availability / reference to effect on (rate
of) activity of bacteria, which begin to die. [1]
Aseptic conditions may not have been achieved. [1] This fall could be due to contamination and
out-competition by ‘wrong’ bacteria (that cannot convert milk to yoghurt). [1]
Temperature may have increased too far from the optimum, [1] which will mean that bacteria die /
enzymes denature. [1]
Oxygenation and agitation may have been insufficient, [1] which has a negative effect on (rate of)
bacterial activity. [1]

Q Answer Marks
4a The diagram should fill as much of the box as possible [1] and be drawn with a sharp pencil to 2
produce clean, continuous lines with no shading. [1]
E.g.
4bi Distance between lines labelled P and Q is 9 mm. 1
4 b ii 9 mm / 0.025 mm [1] = ×360 [1] 2

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CAMBRIDGE IGCSE™ BIOLOGY: PRACTICE EXAM-STYLE TEST 3: ALTERNATIVE TO PRACTICAL ANSWERS

Practice exam-style test 3: Alternative to

1 b ii Any three from: 3

1c Variable 1: Temperature AND 2

1e Role of carbohydrate in plant cells Name of carbohydrate 2

[1 mark for each row]

2b Measuring cylinder. [1] 1

Cambridge IGCSE™ Biology – Martindill © Cambridge University Press 2021 1

2c Any two from: 2

2di Iodine (solution). [1] 1

2 d ii Dropping pipette. [1] 1

Investigation 1 Investigation 2 Investigation 3 Mean

0 7.8 7.8 7.8 7.8

2 5.9 5.8 5.4 5.7

4 7.4 7.2 7.0 7.2

6 9.6 10.7 10.0 10.1

8 20.1 17.0 14.5 17.2

10 11.4 6.4 13.4 10.4

3b Time point = 4 hours. 1

3c Orientation correct: Time / hours on x-axis and pH on the y-axis. [1] 4

3e One pair from: 2

Cambridge IGCSE™ Biology – Martindill © Cambridge University Press 2021 2

4bi Distance between lines labelled P and Q is 9 mm. 1

4 b ii 9 mm / 0.025 mm [1] = ×360 [1] 2

Cambridge IGCSE™ Biology – Martindill © Cambridge University Press 2021 3

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