Preparation and Standardization of Sodium Thiosulfate Solution

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Solutions AACC International Method 70-75.

01
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Preparation and Standardization of Sodium Thiosulfate Solution


Final approval April 13, 1961; Reapproval November 3, 1999

Objective
This method describes a procedure for the preparation and standardization of
0.1N sodium thiosulfate solution.

Apparatus
Drying oven capable of operating at 100°.

Reagents
1. Sodium thiosulfate pentahydrate (Na2S2O3·5H2O).
2. Borax (sodium tetraborate decahydrate, Na2B4O7·10H2O).
3. Potassium dichromate (K2Cr2O7). Dry 2 hr at 100°.
4. Potassium iodide (KI).
5. Hydrochloric acid (HCl), concentrated.
6. Soluble starch-KI solution. Suspend 2 g soluble starch in small quantity
cold water and pour slowly into boiling water with constant stirring. Cool thor-
oughly (or resulting mixture will be dark), add 50 g KI, dilute to 100 ml, and
add 1 drop saturated NaOH solution.

Procedure
For 0.1N solution, dissolve 24.82 g Na2S2O3⋅5H2O and 3.8 g borax (see Note)
and make to 1 liter. Standardize against potassium dichromate as follows:
1. Accurately weigh 0.20–0.23 g K2Cr2O7 and dissolve in 80 ml Cl-free
water.
2. Add 2 g KI and 8 ml concentrated HCl, mix thoroughly, and titrate with
thiosulfate solution, swirling liquid constantly until brown color has changed to
yellowish-green.
3. Add 1 or 2 ml soluble starch-KI solution and titrate until color changes
from blue to light green.

Calculation
Strength of thiosulfate is expressed in terms of its normality.
g K 2 Cr2 O 7 × 1000
Normality of Na 2 S 2 O 3 solution =
ml Na 2 S 2 O 3 × 49.032

Note
Addition of 3.8 g borax to each liter of sodium thiosulfate solution will
prevent bacterial deterioration, and makes it unnecessary to redistill water for
solution. See Ref. 3.

http://dx.doi.org/10.1094/AACCIntMethod-70-75.01
Solutions AACC International Method 70-75.01
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Preparation and Standardization of Sodium Thiosulfate Solution


(continued)

References
1. AOAC International. 1995. Official Methods of Analysis of AOAC International, 16th ed. Method
942.27; Appendix A, p. 7. The Association, Arlington, VA.
2. Kolthoff, I. M., and Sandell, E. B. 1943. Textbook of Quantitative Inorganic Analysis. Macmillan,
New York, pp. 390, 713.
3. Putnam, H. W., Blish, M. J., and Sandstedt, R. M. 1935. Factors affecting maltose values in the
ferricyanide method for diastatic activity. Cereal Chem. 12:494.

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