Acetate Agar: Intended Use

Acetate Agar M1225
Intended Use:
Recommended for the isolation and cultivation of Leuconostoc and Pediococcus species.
Composition**
Ingredients Gms / Litre
Peptone 5.000
HM extract # 5.000
Yeast extract 5.000
Dextrose (Glucose) 10.000
Polysorbate 80 (Tween 80) 0.500
Sodium acetate trihydrate 27.220
Agar 20.000
Final pH ( at 25°C) 5.4±0.2
Agar 20.000
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Meat extract
Directions
Suspend 61.9 grams (the equivalent weight of dehydrated medium per litre) of dehydrated medium in 1000
ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15
lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Leuconostoc is a genus of gram-positive bacteria, which are heterofermentative and are able to produce dextran from
sucrose. These are blamed for causing the stink when creating a sour dough starter. Some species are also capable of causing
human infection (5). Pediococcus is a genus of gram-positive lactic acid bacteria, which are purely homofermentative.
Pediococcus bacteria are usually considered contaminants of beer and wine although their presence is sometimes desired in
beer styles such as Lambic. Certain Pediococcus isolates produce diacetyl, which gives a buttery or butterscotch aroma to
some wines (such as Chardonnay) and a few styles of beer. Pediococcus species are often used in silage inoculants. Acetate
agar was formulated by Whittenbury (6) and then modified by Keddie (3). Peptone, yeast extract, HM extract provide
nitrogeneous and carbonaceous compounds, vitamins and all essential growth nutrients. Polysorbate 80 maintains the surface
tension of the medium to the optimal level. Glucose is the energy source. Sodium acetate serves as a sole source of carbon.
Type of specimen
Food samples and Brewery Samples
Specimen Collection and Handling:

For food samples, follow appropriate techniques for sample collection and processing as per guidelines (4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection.
Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per
established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in
individual safety data sheets.
Limitations :
1. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
2.Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate
the medium for any specific microorganism other than mentioned in the COA based on the user’s unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at
recommended temperature.
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Quality Control
Appearance
Light yellow to beige homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% Agar gel.
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 6.19% aqueous solution at 25°C. pH : 5.4±0.2
pH
5.20-5.60
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 18-48 hours.
Organism Growth
Enterococcus faecalis ATCC 29212 none-poor
(00087*)
Leuconostoc mesenteroides good-luxuriant
ATCC 12291
Pediococcus acidilactici good-luxuriant
ATCC 33314
Key : *Corresponding WDCM numbers.
Storage and Shelf Life

Store dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should
be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic
nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area
protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product
performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must
be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
Reference
1. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
2. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of
Clinical Microbiology, 11th Edition. Vol. 1.1.
3. Keddie R. M., 1951, Proceed. Soc. Appl. Bacteriol., 14:157
4. Salfinger Y., and Tortorello M.L., 2015, Compendium of Methods for the Microbiological Examination of Foods, 5th Ed.,
American Public Health Association, Washington, D.C.
5. Vagiakou-Voudris E., Mylona-Petropoulou D., Kalogeropoulou E., Chant zis A., Chini S., Tsiodra P., Malamou-Lada E., J.
Infect. Dis. 2002;34(10):766-7
6. Whittenbury R., 1965 b, J. Gen. Microbiol., 40:97.
Revision : 03/2020
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate
office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
[email protected] Website: www.himedialabs.com

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Acetate Agar M1225

Specimen Collection and Handling:

Warning and Precautions :

Storage and Shelf Life

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