Instruction Sheets

QWAS
Water Microbiology
Proficiency Testing Scheme

lgcstandards.com/AXIO

Issue No: 20
Issued: 05/10/2023
 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample ID Protocol Page

General Information 3-4
PT-WT-412 WM1V-A 5
PT-WT-413 WM1V-A 5
PT-WT-414 WM1V-B 6
PT-WT-416 WM2V 7
PT-WT-417 WM1V-C 8
PT-WT-418 WM1V-C 8
PT-WT-419 WM1V-C 8
PT-WT-420 WM1V-C 8
PT-WT-421 WM1V-A 5
PT-WT-422 WM1V-D 9
PT-WT-423 WM1V-C 8
PT-WT-424 WM1V-A 5
PT-WT-425 WM1V-A 5
PT-WT-426 WM1V-E 10
PT-WT-427 WM1P 11
PT-WT-428 WM2T 12
PT-WT-429 WM1V-B 6
PT-WT-430 WM2T2V 13
PT-WT-431 WM1T 14

Issue No: 20
Issued on: 05/10/2023
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 QWAS - Water Microbiology Proficiency Testing
Scheme

GENERAL INFORMATION

Storage after receipt

On receipt of the test material, record the date and store as appropriate.

Storage temperature Samples

PT-WT-412 PT-WT-418 PT-WT-423 PT-WT-429

PT-WT-413 PT-WT-419 PT-WT-424 PT-WT-430
Refrigerated
PT-WT-414 PT-WT-420 PT-WT-425
(2 to 8°C)
PT-WT-416 PT-WT-421 PT-WT-426
PT-WT-417 PT-WT-422 PT-WT-428
Frozen (-20°C) PT-WT-431

Please note:
- Conditions for storage after receipt of the sample may differ from the conditions
under which the PT sample was transported
- Stability data have shown that uncooled transport of samples intended to be stored
at 2-8°C does not affect the stability of the test materials for the time of the PT
round
The test material(s) should be analysed in accordance with the deadlines shown on
the website: https://portal.lgcstandards.com

Choice of method or procedure

Participants are expected to use the test method, calibration, or measurement
procedure of their choice. This method should be consistent with the participant’s
normal procedures, for example, duplicate analysis should only be performed if that is
part of the routine analytical process.
Test materials when reconstituted, represent a ‘real’ or ‘neat’ water or effluent sludge
sample, which may or may not contain the target organism(s) at a range of inoculum
levels. Background flora may also be present.
Participants may submit results for some, or all the parameters requested.

Precautions
• Microbiological test materials contain viable micro-organisms and are supplied
on the understanding that the purchaser has suitably competent and qualified
personnel to handle them safely. Test materials must only be opened in a
laboratory by qualified personnel.
• Refer to the Safety Data Sheet for information on the safe handling and disposal
of the test materials

Issue No: 20
Issued on: 05/10/2023
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 QWAS - Water Microbiology Proficiency Testing
Scheme

Reporting Results
• All results should be submitted using PORTAL
• Please go to https://portal.lgcstandards.com
• Login using your Lab ID, username, and password.
• A PORTAL user guide can be downloaded from the help section.

If you need any help at all, please do not hesitate to contact our support team using
the details below or your local LGC representative.
Tel: +44(0)161 762 2500
Email: [email protected]

Issue No: 20
Issued on: 05/10/2023
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 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample codes: Description:

Protocol
1 x 10ml vial
WM1V-A PT-WT-412 PT-WT-413 PT-WT-421
PT-WT-424 PT-WT-425

Back to sample list

Issue No: 20
Issued on: 05/10/2023
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 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample codes: Description:

Protocol
1 x 10ml vial
WM1V-B PT-WT-414 PT-WT-429

Back to sample list

Issue No: 20
Issued on: 05/10/2023
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 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample codes: Description:

Protocol
1 x 10g sludge for Salmonella
WM2V PT-WT-416 detection
1 x 10g sludge for E. coli
enumeration

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Issue No: 20
Issued on: 05/10/2023
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 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample codes: Description:

Protocol
1 x 10ml vial
WM1V-C PT-WT-417 PT-WT-418 PT-WT-419
PT-WT-420 PT-WT-423

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Issue No: 20
Issued on: 05/10/2023
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 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample codes: Description:

Protocol
1 x 10ml vial
WM1V-D PT-WT-422

Back to sample list

Issue No: 20
Issued on: 05/10/2023
Page 9 of 14
 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample codes: Description:

Protocol
1 x 10ml vial
WM1V-E PT-WT-426

Back to sample list

Issue No: 20
Issued on: 05/10/2023
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 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample codes: Description:

Protocol
1 x paper
WM1P PT-WT-427

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Issue No: 20
Issued on: 05/10/2023
Page 11 of 14
 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample codes: Description:

Protocol
2 x 1 tube containing
WM2T PT-WT-428 lyophilised tablet

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Issue No: 20
Issued on: 05/10/2023
Page 12 of 14
 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample codes: Description:

Protocol
2 x 1 vial containing
WM2T2V PT-WT-430 SARS-CoV-2 material
+
2 x 1 tube containing
lyophilised tablet

Back to sample list

Issue No: 20
Issued on: 05/10/2023
Page 13 of 14
 QWAS - Water Microbiology Proficiency Testing
Scheme

Sample codes: Description:

Protocol
1 tube containing
WM1T PT-WT-431 lyophilised material

Step 1 Step 2 Step 3

Prepare at Add 1ml Homogenise
least 5ml sterile using the
sterile deionised pipette
deionised water to the
water tube

Step 4 Step 5 Step 6

Transfer the Homogenise Analyse 1ml
whole content using the with your
to a tube pipette chosen
containing method
4ml sterile
deionised
water

Back to sample list

Issue No: 20
Issued on: 05/10/2023
Page 14 of 14