SureCycler 8800 PCR Brochure
SureCycler 8800 PCR Brochure
SureCycler 8800 PCR Brochure
6 c/sec
the easy choice for flexibility with 96 and 384 well blocks
Quickly convert interchangeable blocks based on your labs needs
Market leading uniformity gives superior results every time you run your protocol
remote access
Use a web-enabled device to monitor your experiment from across the room or across town Networking features come without extra software or hardware purchase required
Pcr Wizard
Improve your research and reduce start-up time with our intuitive protocol wizard Quickly create new protocols tailored to your research in 5 steps
Pre-loaded protocols
Save time using Agilents most popular enzyme protocols pre-loaded on the SureCycler 8800 Easily add new protocols or choose from a list of Agilent developed protocols
Herculase II Fusion DNA Polymerase produced superior yields of 6 kb human genomic DNA fragment at extension times of 15-60 sec/kb. The experiment was conducted using each enzymes recommended buffer and unit concentration.
Amplification of 0.9, 1.7, and 2.6 kb fragments of Human a-1 Antitrypsin gene, and 6 kb fragment of human -gene amplified using Paq5000 Hotstart PCR Master Mix and the closest hot start Taq master mix competitor.
rt Enzymes
AccuScript reverse transcriptase The worlds most accurate reverse transcriptase AffinityScript reverse transcriptase Sensitivity combined with superior yields across a broad temperature range
EntirE Portfolio
SurEly bEttEr reagent Kits
Now its easy to be sure about getting good results
rt-Pcr Kits
Easy-A One Tube rt-PCr System The only one-step RT-PCR system offering both TA/UA cloning and high-fidelity AccuScript PfuUltra II RT-PCR Kit Highest fidelity RT combined with highest fidelity PCR enzyme on the market AffinityScript One-Step RT-PCR Kit Delivers the highest yield and sensitivity of any one step RT-PCR kit
Superior sensitivity with the AffinityScript One-Step RT-PCR Kit. Total RNA at varying concentrations (1, 10 and 100 ng) was amplified for multiple targets, M1: 1 kb DNA Ladder; M2: lambda/HindIII marker
Digestion
4 kb
50
25
10
50
25
10
50
25
10
Template size
Closest Competitor A
Closest Competitor B
48 min 5 min
Total 53 min
Digestion
8 kb
60 min
Total 1 hr 5 min
3.9 kb
12 kb
60 min
(235% faster)
Time
Superior sensitivity with PicoMaxx High-Fidelity PCR System. Amplification of a 3.9 kb a-1 anti-trypsin template compared to Agilents two closest competitors. All reactions were performed according to the manufacturers recommendations.
Different template sizes were mutated in both the QuikChange Lightning Mutagenesis Kit and the original QuikChange Mutagenesis Kit. Times were measured for thermal cycling and digestion.
the agilent Surecycler 8800 gives you the best overall specification package
Specifications
Maximum Block Ramp Rate Block Temperature Range Temperature Accuracy temperature uniformity Gradient Maximum Gradient Volume Capacity Memory Capacity Pre-loaded Protocols PCR Wizard Display Size Display resolution Display type Ports Dimensions Weight Standard Warranty
96-well block
6 C/sec 4 C to 99 C 0.2 (@95 C) 0.4 (@95 C) yes 30 C 10-100l yes yes
384-well block
4 C/sec 4 C to 99 C 0.2 (@95 C) 0.4 (@95 C) yes 30 C to 99 C 30 C 5-25l
Find out more about the full Agilent reagents and consumables portfolio
Strataclone Pcr optimization and Purification PCR Polishing Kits Perfect Match PCR Enhancer StrataPrep DNA Gel Extraction Kits StrataPrep PCR Purification Kits Plasticware Markers and ladders absolutely rna Purification Products Stratacoolers
10,000 protocols
www.agilent.com/genomics/reagents
7 inches 800 x 480 Full touch Screen 2 x uSB 2.0 26 x 41 x 37 cm 12.4 kg 2 year
for more information Learn more or buy online www.agilent.com/genomics/8800 Find an Agilent customer center in your country www.agilent.com/genomics/contactus U.S. and Canada 1-800-227-9770 [email protected] Europe [email protected] Asia Pacific [email protected]
this information is subject to change without notice. Agilent Technologies, Inc. 2011 Printed in the USA, March 25, 2011 5990-7749EN